Publications

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detection of human papillomavirus (hpv) dna in focal epithelial hyperplasia.five focal epithelial hyperplasia (feh) specimens from four patients were examined by southern blot hybridization analysis to determine the specific human papillomavirus (hpv) types present. the histomorphologic features of these specimens were also evaluated and a broad variety of changes including koilocytes, mitosoid cells, ballooning cells and cells showing individual cell keratinization were noted. feh lesions from the three patients sharing a familial relationship demonstrated hpv dna sequ ...19892547947
structure and expression of an integrated human papillomavirus type 16 genome amplified in a cervical carcinoma cell line.a cellular sequence containing the integrated human papillomavirus type 16 genome in a cervical carcinoma cell line qg-u was cloned and analysed. the transcriptionally active viral genome disrupted at the e2 and l2 open reading frames was amplified with its flanking sequences.19892544671
an atypical case of penile carcinoma in situ associated with human papillomavirus dna type 18.we report an unusual case of penile carcinoma in situ in a heterosexual man. the histologic study showed a severe dysplasia, and the typing of human papillomavirus dna, performed at the pasteur institute, disclosed a type 18, which is usually associated with penile and cervical carcinomas. the identification of an oncogenic human papillomavirus type in the penile lesions, such as type 18 found in this case, should lead to a search for cervical carcinoma in the sexual partner.19892541182
dna of human papillomavirus type 16 in dysplastic and malignant lesions of the conjunctiva and cornea.human papillomaviruses are receiving attention for their role in the pathogenesis of cancer, especially cancer of the anogenital tract. although strains of human papillomavirus are associated with benign lesions of the conjunctiva, their association with conjunctival dysplastic lesions and carcinomas has remained unclear. we examined a group of neoplastic lesions of the conjunctiva for the presence of dna sequences for human papillomavirus types 16 and 18, using in vitro gene amplification with ...19892541337
detection of the human papillomavirus 6b e2 gene product in genital condyloma and laryngeal papilloma tissues.we have previously reported that the e2 open reading frame of human papillomavirus type 6b (hpv6b) was expressed in bombyx mori cell line bm-n as a fusion protein with baculovirus polyhedrin. here we show that antibody against this e2-polyhedrin fusion protein recognized a putative gene product as a 49-kda protein in some genital condyloma and laryngeal papilloma tissues, which were associated with hpv6 and hpv11, by immunoblotting analysis. the antibody reacted also with antigens in the nucleus ...19892541557
juvenile and adult laryngeal papillomata: classification by in-situ hybridization for human papillomavirus.we report the application of an in-situ hybridization technique for the demonstration of human papillomavirus (hpv) employing a biotin-streptavidin-polyalkaline phosphatase complex to paraffin processed tissue from a series of patients with laryngeal papillomata. all cases of juvenile papillomata, whether solitary or multiple, proved positive for hpv types 6 and/or type 11. however, only two cases of adult solitary papillomata and five cases of adult multiple papillomata were positive for hpv ty ...19892541952
human papilloma virus type 11 in a fatal case of esophageal and bronchial papillomatosis.we report a case of an apparently healthy 27-year-old man with a fatal course of papillomatosis, originating in the distal part of the esophagus and spreading into the main and intermediate bronchus. human papillomavirus type 11, usually associated with juvenile laryngeal papillomatosis and genital condyloma acuminatum, was detected in the papillomas. in spite of treatment with co2-laser evaporation of the papillomas, and with systemic as well as topical interferon, vp-16 and bleomycin, the papi ...19892543063
human papillomavirus (hpv) infection in seborrheic keratosis.we studied 89 seborrheic keratoses. in four cases, light microscopy and electron microscopy demonstrated that seborrheic keratoses may be associated with human papillomavirus (hpv) infection.19892543229
human papillomavirus types 6 and 16 in cooperation with ha-ras transform secondary rat embryo fibroblasts.using a focus assay we have shown that the entire human papillomavirus type 16 (hpv-16) genome is capable of cooperating with an activated ras oncogene to transform secondary rat embryo fibroblast (ref) cells as indicated by focus formation of unselected cells. however, this assay failed to detect any similar activity with either the whole hpv-6 genome or with subgenomic fragments. in contrast, transformed colonies appeared when g418 (geneticin)-resistant colonies were selected after cotransfect ...19892543741
the multicentric nature of disease related to human papillomavirus infection of the female lower genital tract. 19892543936
detection of human papillomavirus genes in human oral tissue biopsies and cultures by polymerase chain reaction.we have used the polymerase chain reaction to detect dna sequences related to human papillomavirus type 16, by simultaneous priming with oligonucleotides from the e6 and l1/l2 open reading frames of the hpv16 genome. the hpv16-related sequence is present at low levels in normal oral tissue, in addition to biopsies and cell cultures from patients with benign and malignant disease. ultimate analysis of the amplified sequences from the e6(120bp) and l1/l2(173bp) regions of hpv16 was achieved by gel ...19892544219
phosphorylation of the human papillomavirus type 1 e4 proteins in vivo and in vitro.following incubation of hpv 1-induced warts in the presence of [32p] phosphate several of the e4-encoded proteins were found to be radiolabeled. two-dimensional isoelectric focusing sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that the 17k e4 polypeptides had incorporated [32p]phosphate whereas those of 16k were unlabeled. purified e4 gene products were separated by ion exchange chromatography into a large number of different species, which were of similar size but of diff ...19892470193
a synthetic peptide defines a serologic iga response to a human papillomavirus-encoded nuclear antigen expressed in virus-carrying cervical neoplasia.the growing awareness of the role of human papillomavirus (hpv) in cervical carcinoma has triggered a search for uncomplicated detection methods. to define a serologic response to hpv, we synthesized peptides based on sequences deduced from the genome of hpv type 16, the most common malignancy-associated type of hpv. one of these peptides reacted with iga antibodies present in sera from 24 of 33 patients with cervical intraepithelial neoplasia or cervical carcinoma, whereas this peptide reacted ...19892471193
possible cofactors in the etiology of cervical intraepithelial neoplasia. an immunopathologic study.previous work in our department demonstrated a reduction in the numbers of langerhans' cells in cervical epithelium showing histologic changes of human papillomavirus (hpv) infection and cervical intraepithelial neoplasia (cin). in conjunction with a localized reversal of the t4:t8 ratio of t-lymphocytes, that finding provides evidence of the association of epithelial immunosuppression with both hpv infection and cin. to investigate whether that phenomenon occurs primarily because of hpv alone o ...19892478707
a cell mutant that exhibits temperature-dependent sensitivity to transformation by various oncogenes.we previously isolated a fisher rat fibroblast mutant, b812, that has the unique property of temperature-dependent transformation by various oncogenic retroviruses. at the permissive temperature (35 degrees c), this mutant was sensitive to oncogenic transformation and formed foci on a dish at the same frequency as did the parental fibroblast cell line. when kirsten murine sarcoma virus (ki-msv) was applied to the cells, the frequency of focus formation decreased more than 25-fold at the nonpermi ...19892479832
transformation of human cells by oncogenic viruses supports permissiveness for parvovirus h-1 propagation.parvovirus h-1 has been shown to suppress spontaneous and chemically or virally induced tumorigenesis in hamsters. in human cell culture systems propagation of h-1 is restricted to transformed cells, which are killed by h-1 infection, in contrast to normal diploid cells, which are nonpermissive for h-1. by analyzing the permissiveness of a variety of human cells for h-1, it was determined that the majority of tested transformed or immortalized cells which were permissive for h-1 contained the dn ...19892495371
optimization of human papillomavirus genotype detection in cervical scrapes by a modified filter in situ hybridization test.optimal conditions for the screening of cervical scrapes for human papillomavirus (hpv) were investigated by using filter in situ hybridization. since integrated and episomal hpv can be found, cell lines containing viral dna in an integrated form (hpv in caski) or in an episomal state (bk virus-induced hamster tumor cells) were used for optimization experiments. an increase in sensitivity was achieved by alkaline denaturation and neutralization before the specimens were spotted onto the membrane ...19892536384
prognostic significance of the presence of human papillomavirus dna in patients with invasive carcinoma of the cervix.cases of invasive carcinoma of the uterine cervix were analyzed to determine whether the presence or absence of human papillomavirus (hpv) dna in the neoplasms was a contributing factor to their outcome. the presence of hpv dna was evaluated using in situ hybridization on formalin-fixed, paraffin-embedded tissue sections. eighty-five patients with cervical carcinoma who had been surgically evaluated were included in the study. data from these patients was analyzed retrospectively to determine su ...19892536587
study of multiple human papillomavirus-related lesions of the lower female genital tract by in situ hybridization.twenty-six women with multiple human papillomavirus (hpv)-related lesions of the lower genital tract were investigated by immunohistochemistry for the internal genus-specific capsid antigen of hpv and by dna-dna in situ hybridization with 35s-radiolabeled probes for sequences of hpv-6/11, hpv-16, and hpv-18. the vulva was the most frequently affected site in all of these cases; the cervix was the second most frequently affected site. the lesions displayed the features of papillomavirus infection ...19892536630
oncogenic and nononcogenic human genital papillomaviruses generate the e7 mrna by different mechanisms.a new promoter located within e6 was mapped in human papillomavirus type 6b (hpv6b)- and hpv11-containing benign genital condylomata (genital warts). the rna transcribed from this promoter represented the major rna species colinear with open reading frames e6 and e7 and can encode the e7 protein. no equivalent promoter was active in hpv16-containing cancers and cancer-derived cell lines. in those, the major transcripts contained one of two different introns within e6 and the rnas could encode tw ...19892536845
human papillomavirus 16 dna in cervical cancers and in lymph nodes of cervical cancer patients: a diagnostic marker for early metastases?human papillomavirus (hpv) 16 is most prevalent in cervical cancers and also persists in metastases. we examined hpv16-dna-positive primary cancers and several lymph nodes from each of 14 patients to evaluate the use of hpv16 dna as a diagnostic marker for the detection of early node involvement. the hpv16 dna was exclusively integrated in 39% of the primary cancers, predominantly episomal in 36%, and integrated and extrachromosomal to a similar extent in 25%. thirteen of 16 involved lymph nodes ...19892536006
[biological significance of hpv sequences integrated into cancer cells].in cervical cancer with integrated human papillomavirus (hpv) type 16 or 18 dna, e6 and e7 regions of hpv 16 or 18 were always present, suggesting that these regions play an important role in development of cervical cancer. structure and biological activity of cdnas representing two types of hpv 18 transcripts in hela cells were analyzed. three open reading frames (e6, e6* and e7) were identified. these open reading frames were introduced into the expression vectors. among them, e7 alone provide ...19892539785
genital human papillomavirus infections.genital human papillomavirus (hpv) infection is an increasingly common sexually transmitted disease that has been associated with carcinoma of the cervix. genital warts are easily diagnosed by clinical criteria; however, the more prevalent subclinical infections generally require laboratory diagnosis. satisfactory methods for culture of hpv do not exist, and cytologic and histopathologic methods are the most widely available diagnostic techniques. detection of hpv dna is a more sensitive method, ...19892551567
cytopathology of sexually transmitted disease.although other methods exist for routine identification of most sexually transmitted diseases, the pathologist can often recognize the etiologic agents or their cytologic effects on papanicolaou (pap) smear. useful clinical information requires both the ability to recognize these effects and knowledge of their limitations. pap smear changes characteristic of trichomonas vaginalis, chlamydia trachomatis, herpes simplex virus, gardnerella vaginalis, candida sp., and human papillomavirus infections ...19892551568
two independently transforming functions of human papillomavirus 8. 19892551581
immunological status to epstein-barr virus and cytomegalovirus in patients with genital condylomata.serological patterns against epstein-barr virus (ebv) and cytomegalovirus (cmv) were determined in patients with genital condylomata (gc). the ig g antibody values to eb-induced virus capsidic antigens (vca), early antigens (ea) and ig m to vca were significantly higher in the study group than in the controls. moreover, the concomitant presence of ebv-ig g anti-vca greater than or equal to 1/320, ebv-ig g anti-ea greater than or equal to 1/20 and ebv-ig m anti vca greater than or equal to 1/20 w ...19892551722
detection of human papilloma viruses in paraffin wax sections with biotinylated synthetic oligonucleotide probes and immunogold staining.human papilloma virus was detected by in situ hybridisation in routinely processed paraffin wax sections using a synthetically produced oligonucleotide probe, end-labelled with biotin, and amplified with anti-biotin-immunogold silver staining (anti-biotin-igss). this system proved more sensitive than amplification with streptavidin-biotinylated alkaline phosphatase for detecting human papilloma virus type 16 in cervical tissues. the method was successfully combined with antigen staining for papi ...19892551931
genital human papillomavirus infection in panama city prostitutes.little is known of the natural history of genital human papillomavirus (hpv) infections in women from high-risk populations. samples were collected from 183 panama city prostitutes and assessed for hpv (filter in situ dna hybridization) and for sexually transmitted agents. the cohort was followed for 8 mo; 51% of subjects completed four monthly return visits and 16% were sampled eight times. the proportion of women found infected with hpv increased significantly with increasing numbers of consec ...19892551974
localization of human papillomavirus type 16 dna using the polymerase chain reaction in the cervix uteri of women with cervical intraepithelial neoplasia.the localization of human papillomavirus type 16 (hpv-16) dna throughout the cervix uteri of women with cervical intraepithelial neoplasia (cin) was studied by utilizing the polymerase chain reaction technique directly on histologically defined sections of paraffin-embedded cervical tissue obtained by conizations. hpv-16 dna was detected only in the sections that contained cin lesions and/or koilocytes. no hpv-16 dna was detected in sections that contained only normal epithelium. this is in acco ...19892552002
heterogeneity of the human papillomavirus group. 19892552162
human papillomavirus type 29 (hpv-29), an hpv type cross-hybridizing with hpv-2 and with hpv-3-related types.the cloning and partial characterization of human papillomavirus (hpv) type 29 is presented. by hybridization analyses, this virus appears to be related to hpv types associated with common warts and hpv types associated with flat warts.19892552165
human papillomavirus (hpv) type 50, a type associated with epidermodysplasia verruciformis (ev) and only weakly related to other ev-specific hpvs.the cloning and partial characterization of the genome of human papillomavirus type 50 is presented. alignment of the genomic map with that of human papillomavirus type 5, with which it is only weakly related, was permitted by partial dna sequence analysis.19892552168
a rapid dna probe test for detecting human papilloma virus types 6/11 and 16 in biopsy specimens.a simple, rapid and sensitive non-radioactive in situ hybridization assay for human papillomavirus (hpv) has been developed and used to detect hpv 6/11 and hpv16 dna in ano-genital biopsy specimens. a comparative study to determine the sensitivity and the specificity of this assay relative to southern blot hybridization assays was performed using one hundred biopsy specimens. the sensitivity of the in situ hybridization assay was 88% for hpv type 6/11 and 89% for hpv type 16. the specificity of ...19892552303
characterization of cdnas of spliced hpv-11 e2 mrna and other hpv mrnas recovered via retrovirus-mediated gene transfer.human papillomaviruses (hpvs) are associated with hyperproliferations of cutaneous or mucosal epithelium. these viruses cannot be propagated in any cell culture system. because cloning cdna copies of hpv mrnas recovered from human lesions has met with only very limited success, the characterization of hpv mrnas has been problematic. using the moloney murine leukemia virus vector system (c.l. cepko, b.e. roberts, and r.c. mulligan, 1984, cell 37, 1053-1062), we have recovered cdnas of spliced e2 ...19892552658
human papillomavirus type 31 infection of the uterine cervix in england.a total of 452 uterine cervical scrapes, from two centres in england, has been examined by dot blot hybridisation for the presence of human papilloma virus (hpv) types 6, 11, 16, 18 and 31. hpv dna was found in 36.5% samples. twenty-nine of the women were infected with more than one type. of those with detectable hpv, 14.5% had hpv type 31 infections. this type appears to cause significant infection of the genital tract in england.19892545788
progesterone and glucocorticoid response elements occur in the long control regions of several human papillomaviruses involved in anogenital neoplasia.we have previously identified in the long control region of the genome of human papillomavirus type 16 (hpv-16) a dna segment which functions as a cell-type-specific enhancer as well as mediating glucocorticoid response. it contains multiple transcription-factor-binding sites, including several for nuclear factor i and one for the glucocorticoid receptor, which binds to the partially palindromic sequence tgtacannntgtcat. we report here that this sequence element, when separated from the surround ...19892545902
chromatin structure and transcriptional regulation of human papillomavirus type 18 dna in hela cells.mapping analysis of the nucleosomal organization of integrated human papillomavirus type 18 (hpv18) dna in hela cells reveals a very prominent nuclease-hypersensitive site within the viral noncoding regulatory region that harbors transcriptional control sequences and coincides with most of the 5' ends of the cytoplasmic early mrnas. moreover, it is shown that the conserved coamplified 5' cellular flank, common to all hpv18 copies in hela cells and located close to the virus-cell integration site ...19892548528
differentiation-linked human papillomavirus types 6 and 11 transcription in genital condylomata revealed by in situ hybridization with message-specific rna probes.human papillomaviruses (hpvs) infect specific human epithelial tissues. because viral propagation in cultured cells has not been achieved, studies of hpv genetic activities have been difficult and rely largely on analyses of patient specimens by conventional biochemical methods. hpv type 6 and type 11 infections often result in genital warts (condylomata acuminata). structural mapping of rnas from such warts reveals that they use alternative promoters, splice sites, and polyadenylation sites to ...19892549716
human papillomavirus and epithelial ovarian neoplasia.in contrast to the strong association between human papillomavirus (hpv) and squamous cell carcinoma of the lower female genital tract, no viral dna had been found in epithelial ovarian carcinoma. recently, however, r. h. kaufman, j. bornstein, a. n. gordon, e. adam, a. l. kaplan, and k. adler-storthy [gynecol. oncol. 27, 340-349 (1987)] reported the detection of hpv-6 dna by in situ hybridization in 10 of 12 patients with advanced epithelial ovarian carcinoma. to further investigate the possibl ...19892548934
selective detection of human papilloma virus dnas by specific synthetic dna probes.specific oligodeoxynucleotide probes ranging from 20 to 35 nucleotides were defined to differentiate each of the hpv1a, 5, 6b, 8, 11, 16, 18 and 33. they were chosen using computer programs developed to compare simultaneously several 8000 bp long dna sequences. sequences common to all and to specific groups of the hpv dna were also selected. specificity of 32p-labelled probes for hpv6b, 11, 16, 18 and 33 was demonstrated and the sensitivity of the assays was evaluated by filter hybridization wit ...19892549406
[intralesional therapy using recombinant interferon alpha 2 b in lesions of the uterine cervix caused by human papilloma virus].a clinical study on 20 patients with uterine cervix human papilloma virus (hpv) lesions treated with intralesional recombinant alpha 2b interferon (3 millions i.u.) three times a week for three weeks where possible, is presented. after one year from treatment, lesions were cured or stabilized in 18 patients (90%); progression was observed in 2 patients (10%) for which reason traditional surgical therapy was performed.19892549455
epidemiology of human papillomavirus (hpv) infections and their associations with genital squamous cell cancer. review article.reliable assessment of the epidemiology of genital hpv infections is hamphered by a number of technical problems. because of the lack of tissue-culture systems, methods based on morphological approaches (colposcopy, cytology and histopathology) play a central role in hpv diagnosis. even dna-hybridization techniques and the recently introduced dna amplification with pcr are extremely difficult to standardize, and are thus subject to major interlaboratory variation. further confusion in the field ...19892556164
sensitivity of in situ hybridization for detection of human papillomavirus (hpv) as studied in hpv type 16-transfected cells. 19892556488
[two cases of oral leukoplakia possibly associated with human papillomavirus].two cases of oral leukoplakia were studied immunohistologically and ultrastructurally. by means of avidin-biotin peroxidase complex technique using papillomavirus specific antibody, distinct nuclear staining of epithelial cells of middle and upper layers was observed in both cases. further evidence for the presence of human papillomavirus was obtained by the electron micrograph, which revealed virus-like particles (30-40 nm in diameter) within the nucleus.19892559216
genital human papillomavirus infection. 19892544328
[occurrences of human papilloma virus dna in cervical carcinomas].out of 16 cases involving a cervical carcinoma that were investigated by southern blot hybridization, found were human papilloma virus (hpv) types 16 and 18 dna sequences in 8 (50%) and in one (6.3%), respectively. six out of the 8 hpv 16-positive specimens were from squamous cell carcinomas, one was from an adenocarcinoma, and the remaining specimen was from an argyrophil small cell carcinoma. in 7 out of 9 hpv-positive specimens, the viral sequences were integrated in the tumor cell genome, wh ...19892550685
chronic cavitary respiratory papillomatosis.a 19-year-old white man with multiple recurrences of respiratory papillomatosis was admitted for recurrent left lower lobe pneumonia and lung abscesses. he was found to have a single large laryngeal papilloma, widespread bronchial papillomatosis, and large cavitary lesions of the left lower lobe. a lobectomy was performed. the smooth-walled, squamous-lined cavities contained large numbers of papillomas, which were strongly positive for human papillomavirus type 11 by in situ dna hybridization. f ...19892552955
sexually transmitted cancers and viruses.both cervical cancer and its precancerous state cervical intraepithelial neoplasia (cin) have the characteristics of being sexually transmitted. formerly herpes simplex virus (hsv) but more recently human papillomavirus (hpv) which both infect the cervix have been implicated in causation. the role of these viruses as possible initiators of cancer or as potential cofactors of cocarcinogens is discussed at the molecular level within the context of the disease process.19892550096
[warts and epidermoid carcinoma after renal transplantation].kidney transplant recipients suffer in the long-term from several cutaneous disorders linked to the transplantation. we had the opportunity to observe several patients presenting with pre-epitheliomatous keratoses and cutaneous carcinomas associated with warts. we report herein on five cases that were subjected to a clinical, histological and virological study. material and methods. clinical and histological report. the patients were referred to use by the kidney transplantation department of th ...19892545155
human papilloma virus infection and its relationship to skin cancer in a group of renal allograft recipients. 19892545416
virology of human papillomavirus. 19892544331
detection of human papilloma virus (hpv) dna in genital biopsy specimens by in situ hybridization with digoxigenin-labeled probes.the presence of human papillomavirus (hpv) nucleotide sequences in paraffin sections of genital biopsies was examined by in situ hybridization using non-isotopic, digoxigenin-labeled probes representing hpv types 11, 16 and 18. digoxigenin-labeling of the probes was performed using dna labeling and a commercially provided detection kit. hybridization was performed under stringent conditions. the hybrids were detected by using anti-digoxigenin alkaline phosphatase conjugate and visualized with en ...19892559923
detection by antibody probes of human papillomavirus type 6 e5 proteins in respiratory papillomata.we have demonstrated the expression of proteins arising from the e5a and e5b open reading frames (orfs) of human papillomavirus type 6c (hpv-6c) in respiratory tract papillomata. recombinant plasmids were constructed to express the orfs in the bacterial vectors path and prit2t. fusion proteins were purified and injected into rabbits to produce polyclonal antibodies. characterized antibodies generated against these fusion proteins were used in immunoperoxidase assays to identify the presence and ...19892559954
[detection of human papillomavirus genome in nasolaryngeal papillomas using digoxigenin labeled dna probes].it is being reported that human papillomavirus (hpv) has been implicated in the pathogenesis of various neoplastic lesions of the genital organs. to investigate the etiological role of hpv and its types in nasolaryngeal papillomas, we retrospectively analyzed hpv genomes by nucleic acid hybridization methods; for detecting dna and mrna, we employed the recently developed nonradioactive (digoxigenin labeled) dna probes and compared the results by radioisotope methods. in total, 43 cases of papill ...19892559960
flow cytometric analysis of dna content in cervical lesions.flow cytometry is used to screen gynaecologic specimens for cervical intraepithelial neoplasia (cin) and its precancerous lesions, possibly associated with human papilloma virus (hpv), in order to investigate the role of aneuploidy as a biological marker in hpv and cin lesions. a total of 299 cervical samples was collected by scraping and the cellular dna content was measured using the propidium iodide staining procedure. two groups of patients were studied; a group of 142 negative controls for ...19892560541
diagnosis of human papillomavirus infection in uterine cervix by immunoperoxidase technique.the present study detected human papillomavirus (hpv) genus-specific antigen in paraffin section of chronic cervicitis tissue by peroxidase-antiperoxidase (pap) technique. the results showed that hpv antigen was detected in 34 of 101 specimens (33.66%). all the normal control sections were negative. the antigen positive nuclei were found in the koilocytes of the upper layers of the epithelium. antigen positive cells may display a uniform or a scattered pattern. the pathologic feature of hpv infe ...19892561299
perspectives on research and diseases of the tropics: an asian view.diarrhea, pneumonia, and malnutrition account for most of mortality and morbidity in children in developing countries. the expanded program of immunization (epi) is making progress with more than 50% of children under the age of 1 year receiving vaccination against the 6 epi-listed diseases. the eradication of poliomyelitis by 2000 is realistic, so that the world could be smallpox- and polio-free by the 21st century. in july-august 1988 a cholera epidemic erupted in delhi, india in which sever ...19892698093
cervical intraepithelial neoplasia in lymphoma patients: a cytological and colposcopic study.twenty-seven patients with hodgkin's (n = 19) and non-hodgkin's (n = 8) lymphomas underwent cytological and colposcopic screening of the uterine cervix. colposcopically directed cervical punch biopsies were taken from all patients in whom a colposcopic abnormality was detected. lymphoma patients were compared with 79 controls with normal cervical cytology and no known haematological abnormality. colposcopically directed punch biopsies were taken from the cervical transformation zone of all contr ...19892713244
[infection by the human immunodeficiency virus (hiv) in french guyana. dermato-venereologic problems].dermato-venereal manifestations in hiv infection and its severe evolution stage, aids, is of particular importance in tropical zones: we may be suspicious of the viral infection and consequently to request serologic tests to confirm it. we get an explanation of the virus transmission during heterosexual relations by the frequent occurrence and importance of the genital manifestations, leading to consider aids as a true sexually transmitted disease. beside the classical opportunistic infections, ...19892725241
detection of human papillomavirus type 11 dna in a conjunctival squamous cell papilloma by in situ hybridization with biotinylated probes.a 44-year-old man presented with an exophytic papillomatous growth in the tarsal conjunctiva of his right eye. the tumour was excised and subjected to histological examination. on light microscopy, a squamous cell papilloma without signs of dysplasia was disclosed, because the entire tissue block was cut into sections, the performance of a conventional in situ hybridization for detection of human papillomavirus (hpv) dna was not possible. an alternative approach was used to demonstrate the prese ...19892801046
[oral contraceptives and cancer].recently some studies have shown an association between the pill and increased risk of breast cancer and cancer of the cervix. several well- designed prospective studies indicated that there was an increased risk of breast cancer for women without children with menarche before 13 who took the pill for many years. a control case study of 407 breast cancer victims compared 424 controls found that the risk of women who had taken hormonal contraceptives was double, however, the duration of use, ag ...19892814116
[assessment of human papilloma virus colposcopic findings of the cervix (in the italian colposcopic classification].hpv-induced flat condylomatosis is not only much more frequently observed on the hexocervix than the florid type, but it also induces colposcopic changes in the epithelium without completely altering its morphology. hpv-induced changes have now been largely identified and are recognisable in colposcopy. they can therefore be classified in the various patterns that characterise colposcopically the transformation epithelium, with undeniable advantages not only from the point of view of their corre ...19892671807
human papilloma virus infections.genital warts are believed to be caused by human papilloma viruses and to be sexually transmitted. the viruses are classified by dna types, which appear to cause different types of disease. the choice of treatment, and usually its success rate, vary according to the type of disease and its location.198921248973
ligneous conjunctivitis: an ophthalmic disease with potentially fatal tracheobronchial obstruction. laryngeal and tracheobronchial features.ligneous conjunctivitis is a rare disease of unknown cause characterized by pseudomembranous, fibrous, woody, plaquelike deposits on the conjunctiva. the disease appears to be hereditary and/or familial. deposits similar to those found in the eye occur in the larynx, tracheobronchial tree, nose and nasopharynx, and vagina. when these lesions occur in the larynx and tracheobronchial tree, voice change and potentially life-threatening obstruction and pulmonary disease may occur. this paper reports ...19902195957
risk of cervical cancer among wives of men with carcinoma of the penis.the relationship between carcinomas of the penis and cervix uteri was analyzed in married couples. the final series comprised 239 patients with squamous cell carcinoma of the penis and their 224 wives. two wives were found to have squamous cell carcinoma of the cervix uteri. the expected number of cervical cancers within the group of wives was 1.88, the relative risk thus being 1.05 (95% confidence interval 0.13-3.8). the incidence of condylomas has increased during the recent decades, while tha ...19902206567
cervical intraepithelial neoplasia iii in an adolescent with bowenoid papulosis.bowenoid papulosis (bp) is a cutaneous condition of the external genitalia seen primarily in young adults. evidence supports an etiologic role of human papillomavirus (hpv), particularly type 16. hpv-16 is also associated with an increased risk for cervical intraepithelial neoplasia (cin) and invasive carcinoma. a 17-year-old female, referred to the adolescent dysplasia clinic with a diagnosis of condyloma acuminatum, was presented. she had multiple smooth, shiny, brown 3- to 4-mm papules on the ...19902175743
cell differentiation-related gene expression of human papillomavirus 33.the gene expression of human papillomavirus (hpv) 33, which can be detected both in benign and malignant genital tumors, was analyzed in a cervical condyloma acuminatum by in situ hybridization using open reading frame-specific rna probes. viral mrna concentrations increased with the degree of differentiation of the keratinocytes. the probes for reading frames e4 and e5 generated the most intense signals. the patterns of the specific viral mrnas were very similar to those in condylomas induced b ...19902175837
[detection of human papillomavirus (hvp) 16/18 infections of the vaginal uterine cervix in females before and following conisation].280 women have been tested by fish to detect an hpv 16/18-infection of the portio uteri. in a group of 133 women treated by conisation (all with a history of abnormal smear and colposcopy) 67 (50%) were positive for hpv 16/18 dna before the operation. follow up examinations of 20 cases with positive hpv 16/18 test before conisation, showed 6 and 12 months after operation only in 3 cases a persistence of hpv 16/18 infection. only 5 (6.4%) of 78 women with a history of conisation and diagnosis cin ...19902176023
use of cervical cytobrush samples for dot-blot detection and southern blot typing of human papillomaviruses using subgenomic probes.the use of cytobrush samples for the detection and typing of cervical human papillomavirus (hpv) infections was analyzed. the average yield of squamous cells from cytobrush samples was on the order of 10(6) cells when the sample was collected in a lytic collection buffer, which was approximately double the content of (1) samples collected in ethanol and (2) an average biopsy specimen. the material obtained could be used for sensitive detection and typing of hpv infections using, respectively, a ...19902176501
colposcopy, punch biopsy, in situ dna hybridization, and the polymerase chain reaction in searching for genital human papillomavirus (hpv) infections in women with normal pap smears.to assess the prevalence of hpv infection in the genital tracts of women with normal pap smears, a random series of 109 women was reexamined using colposcopy, a further pap smear, and punch biopsies taken from the cervix (in 33 cases), vagina (212 cases), and anus (20 cases). the biopsy material was examined using routine histological investigations, in situ hybridization (ish) with a 35s-labelled dna probe cocktail (hpv 6, 11, 16, 18), and the polymerase chain reaction (pcr) to detect hpv dna. ...19902176670
the detection of human papillomavirus (hpv) in a case of minimal deviation adenocarcinoma of the uterine cervix (adenoma malignum) using in situ hybridization.hpv-16 and hpv-18 dna sequences were detected in a case of minimal deviation adenocarcinoma (mda), a rare pathological type of uterine cervical cancer. this result suggests hpv to be associated with mda as well as with other pathological types of cervical cancer.19902178197
human papillomavirus in oesophageal squamous cell carcinoma.thirty seven cases of oesophageal squamous cell carcinoma were studied by applying dna slot blot analysis and in situ hybridisation using type specific probes for hpv 6, 11, 16 and 18. cases of condyloma accuminata, cervical carcinoma, and laryngeal papilloma were used as controls. blocks including areas of invasive carcinoma, intraepithelial neoplasia, and normal epithelium were studied in each case. no hpv genome was detectable in any of the oesophageal cases. it is concluded that these types ...19902175754
human papillomavirus associated with keratoacanthomas in australian patients. 19902175166
detection of human papillomavirus dna in laryngeal squamous cell carcinomas by polymerase chain reaction.the presence of human papillomavirus genomes-16 and -6b in metastatic cervical lymph nodes was examined in 34 cases of laryngeal carcinomas by means of polymerase chain reaction, which had been fixed in formalin and embedded in paraffin. human papillomavirus dnas extracted from paraffin-embedded tumor tissues were used for polymerase chain reaction with amplification of the e6 region of human papillomavirus genome-16 and the e1 region of human papillomavirus genome-6b. human papillomavirus genom ...19902348745
cancer incidence correlations: genital, urinary and some tobacco-related cancers.bivariate correlations between sex-specific age-adjusted incidence rates of 10 genital and urinary tract cancer sites and lung cancer were calculated. the data base used was that published in the 5th edition of the series cancer incidence in five continents. the purpose of the analyses was to test the consistency of the data with the hypothesis of a common risk factor for genital cancers and other cancers of the urinary tract and for lung and genital cancers. the results indicate that the incide ...19902384268
estrogen receptor localization in normal and neoplastic epithelium of the uterine cervix.to investigate the estrogen receptor (er) status of cells during carcinogenesis of the uterine cervix, the immunohistochemical reactivity for a monoclonal anti-er antibody (h 222) was studied in 26 normal cervical specimens, 21 cases of cervical intraepithelial neoplasia (cin), and 21 cases of invasive cervical carcinoma. in addition, the presence of human papillomavirus (hpv) dna (types 6/11, 16/18, or 31/33/35) was analyzed by in situ hybridization. in the normal cervix, basal cells of the squ ...19902249202
genetic screening with exfoliated cervicovaginal cells collected by a noninvasive lavage technique performed during routine gynecologic cancer screening.we have investigated and established the feasibility of the use of exfoliated cervicovaginal cells as an abundant source of genetic material. we routinely use this material for the detection by molecular hybridization of human papillomavirus infection of the cervix. we have analyzed deoxyribonucleic acid isolated from cervicovaginal cells by southern blot hybridization to detect a common restriction fragment length polymorphism on chromosome 13. we suggest that in the near future most, if not al ...19902316565
clinical significance of hyperkeratosis and parakeratosis in otherwise negative papanicolaou smears.to ascertain the clinical significance of hyperkeratosis and parakeratosis in otherwise negative papanicolaou smears, we retrospectively reviewed 96 patients with these findings seen during a 32-month period. evaluation included repeating the smear and performing colposcopy in all patients. colposcopically directed biopsy and endocervical curettage were performed when appropriate. only one patient had human papillomavirus (hpv) infection. the isolated finding of hyperkeratosis or parakeratosis w ...19902227586
comparison of southern transfer hybridization and dot filter hybridization for detection of cervical human papillomavirus infection with types 6, 11, 16, 18, 31, 33, and 35.a commercial dot filter hybridization kit (virapap kit) was compared with southern transfer hybridization for the detection of seven types of human papillomavirus (hpv) in cervical specimens from 450 consecutive females attending a sexually transmitted diseases clinic. in comparison with southern transfer hybridization, performed with the same probes used in the dot filter kit, the sensitivity, specificity, and positive and negative predictive values of dot filter hybridization were 90%, 94%, 74 ...19902173397
[asymptomatic and subclinical human papillomavirus infections of the genital organs and anus in males]. 19902173853
cytologic correlates of cervical papillomavirus infection.a fundamental question in papanicolaou smear screening is the specificity of cytologic criteria for the recognition of genital human papillomavirus (hpv) infection. to address this problem, we conducted a two-phase study of routinely screened women to determine the efficiency with which cytologic findings identified the presence of hpv dna, focusing on the criteria for identifying smears as "atypical." in phase 1, 25 of 290 (8.6%) smears were designated atypical, but only 3 (12%) of the samples ...19902174025
vestibular papillae of the vulva. lack of evidence for human papillomavirus etiology.vestibular papillae of the vulva are usually considered as anatomical variants of the vestibular mucosa. clinically, however, they are quite often interpreted as condylomata acuminata and recent studies have suggested that they could be related to human papillomavirus infection. this prompted us to search for human papillomavirus dna using the southern blot hybridization technique, by analyzing biopsy specimens taken from 29 patients who presented with papillae of the vestibular mucosa. human pa ...19902175164
[molecular biologic study on the carcinogenesis of hpv in uterine cervical cancer and related lesions--analysis of hpv types 16, 18 e6/e7 gene mrna].recent molecular biologic studies have shown that some type of human papillomavirus (hpv) is closely associated with uterine cervical cancer. in order to investigate the relationship between hpv dna and cervical cancer, we studied the carcinogenesis of hpv in cervical cancer and related lesions by dot blot hybridization (d.b.h.), southern blot hybridization (s.b.h.), in situ hybridization (i.s.h.) and polymerase chain reaction (p.c.r.). we also followed some hpv dna, mrna positive or negative ca ...19902172419
effect of perilesional injection of alpha-interferon on cervical intraepithelial neoplasia and associated human papillomavirus infection.in this controlled prospective study, 14 patients with cervical intraepithelial neoplasia (cin) were observed for one month to exclude spontaneous regression, and then seven patients were treated twice-weekly with perilesional injections of alpha-interferon. both groups were monitored colposcopically, and advised to use the contraceptive sheath. cervical smears and biopsies for histology and viral studies were taken before and after the study. there was an improvement of six out of seven of the ...19902172535
[studies on pathogenesis of cervical carcinoma based on the analysis of growth and differentiation mechanism of cervical epithelium].recently cervical cancer is defined as a sexually-transmitted disease, and human papillomavirus (hpv) has been focused as one of its etiologic agents. it is known that cervical cancer is extraordinarily rare in non-human mammals that have the estrous cycle. in contrast, cervical cancer is frequent in human beings which have lost the estrous cycle, and subsequently evolved a sexual behavior irrespective of the menstrual phase. therefore, upon the hypothesis that the estrous cycle is a period prot ...19902172418
the presence of human papillomavirus (hpv) in solitary adult laryngeal papillomas demonstrated by in-situ dna hybridization with sulphonated probes.human papilloma virus (hpv) types 6 and 11 have been repeatedly demonstrated in multiple laryngeal papillomas, and there is little doubt that these lesions are caused by hpv. it has been clearly demonstrated in recent reports that the clinical course of solitary adult onset laryngeal papillomas is entirely different from that of multiple papillomas of juvenile as well as of adult onset. we here report the presence of hpv types 6 and 11 in 19 out of 20 solitary papillomas from 16 patients, while ...19902171818
human cervical cells immortalized in vitro with oncogenic human papillomavirus dna differentiate dysplastically in vivo.human papillomavirus (hpv) types 16 and 18 are associated with cervical dysplasia and carcinoma. in vitro integration of hpv-16 or hpv-18 dna into cultured human cervical cells results in their immortalization. in this study, in vivo differentiation of human cervical cells immortalized with recombinant hpv-16 or hpv-18 dna was studied by implanting confluent monolayers of hpv-16 and hpv-18 dna immortalized cell lines under dorsal skin flaps of immunodeficient mice. grafts of a human cervical can ...19902172120
the dna-binding domain of hpv-16 e2 protein interaction with the viral enhancer: protein-induced dna bending and role of the nonconserved core sequence in binding site affinity.we expressed the carboxy-terminal portion of the e2 open reading frame (orf)-encoded protein of human papillomavirus type 16 (hpv-16) and purified it to near homogeneity. using dnase i footprinting techniques, we show that like the homologous protein from bovine papillomavirus type 1 (bpv-1), hpv-18, hpv-11, it binds dna at the enhancer consensus motif accn6ggt. base and phosphate backbone contact points were determined using methylation protection and interference and ethylation interference as ...19902154890
study of stringency conditions for human papillomavirus dna detection on cell lines, frozen and paraffin-embedded tissue sections by in situ hybridization with biotinylated probes.in situ hybridization was mainly used for typing human papillomavirus (hpv) in paraffin-embedded or frozen sections under stringent conditions (sc). we tested 5 different conditions of stringency with biotinylated hpv 1, 2, 16 and 18 probes on 3 cell lines (sihà and caski with hpv16, hela with hpv18) by varying the concentration of formamide in the hybridization mixture and washings in order to determine the stringency conditions to be used to assess the presence of hpv and its typing: a-low str ...19902158491
the nuclear oncoproteins: rb and p53.a novel class of oncogene has been recognised whose loss-of-function results in the expression of the malignant phenotype. two examples of such genes are the human retinoblastoma predisposition gene (rb1) and the gene encoding the cellular protein p53. these genes are thought to regulate and limit normal proliferation of cells and, as a consequence, can suppress tumorigenicity when introduced into transformed cells. they are hence frequently described as 'tumour suppressor genes'. both rb1 and p ...19902151736
mutational analysis of human papillomavirus type 16 e7 functions.the human papillomavirus type 16 e7 gene encodes a nuclear oncoprotein (98 amino acids [aas] long) consisting of three regions: regions 1 (aas 1 to 20) and 2 (aas 21 to 40), which show high homology to the sequences of conserved domains 1 and 2, respectively, of adenovirus e1a; and region 3 (aas 41 to 98) containing two metal-binding motifs cys-x-x-cys (aas 58 and 91 to 94). we constructed aa deletion (substitution) mutants and single-aa substitution mutants of e7 placed under the control of the ...19902152813
in situ hybridization analysis of human papillomavirus dna segregation patterns in lesions of the female genital tract.various histologic features may be used to divide human papillomavirus (hpv)-related lesions of the genital tract into two groups: condylomata and "low-grade" or grade 1 cervical intraepithelial neoplasias (cin 1) versus "high-grade" or grade 2 and 3 intraepithelial neoplasias. using in situ hybridization analysis we correlated hpv dna type with histologic features in 350 biopsies of lesions from the cervix, vulva, and perianal region. hpv dna was most commonly found in vulvar and perianal condy ...19902153624
oral hairy leukoplakia.oral hairy leukoplakia was first described in homosexual men infected with the human immunodeficiency virus. it is thought to be caused by infection with both the epstein-barr virus and human papillomavirus. we report 59 cases of oral hairy leukoplakia. the disease was diagnosed in patients in all risk groups and was categorized in all classes of the walter reed classification without significant differences in prevalence. epstein-barr virus could be demonstrated in all tissue samples examined; ...19902153716
longitudinal study of human papillomavirus infection of the female urogenital tract by in situ hybridization.the recurrence and/or persistence of human papillomavirus (hpv)-associated lesions constitute a well-recognized clinical problem; yet, few studies have demonstrated and typed the hpv within these recurrence lesions. a patient with a 17-year history of anogenital condylomata presented with an unusual bladder tumor that had histologic features of hpv infection. this prompted a longitudinal analysis of hpv dna in archival material from these urogenital lesions. colorimetric in situ hybridization wa ...19902154168
natural cell-mediated cytotoxicity against various target cells in patients with epidermodysplasia verruciformis.natural cell-mediated cytotoxicity of peripheral blood mononuclear cells was studied in eight patients with epidermodysplasia verruciformis induced by human papillomaviruses specific for epidermodysplasia verruciformis and in five patients with epidermodysplasia verruciformis-induced exclusively by human papillomavirus type 3. nine patients with various cutaneous warts and 25 age- and sex-matched healthy persons were control subjects. natural cell-mediated cytotoxicity against both k-562 erythro ...19902155952
an alternate view of the management of cervical human papillomavirus infections. 19902155956
evaluation of oral and laryngeal specimens for human papillomavirus (hpv) dna by dot blot hybridization.human papillomavirus (hpv) is an infectious agent which is increasingly associated with mucosal cancers, in particular, cancer of the cervix. the present investigation was undertaken in an attempt to determine whether the virapap human papillomavirus (hpv) dna detection kit, originally designed for identification of hpv in exfoliated cervical cells could be employed in detection of hpv in biopsies of oral and laryngeal tissue samples, specifically oral squamous cell carcinomas, epithelial dyspla ...19902156069
human papillomavirus infection.the human papillomavirus, well known as the etiologic agent of warts, has recently received much attention in the medical literature for its association with various cancers. this article discusses the virology, epidemiology, pathogenesis, immunology, clinical manifestations, and therapy for human papillomavirus infection. some newer techniques for identifying human papillomavirus in tissue, based on recent advances in molecular biology, are also covered. two final topics addressed are human pap ...19902156916
absence of human papillomavirus (hpv) dna sequences in epithelial ovarian carcinoma. 19902157639
detection of human papillomavirus in skin and genital lesions of renal allograft recipients by in situ hybridization.renal allograft recipients have an increased incidence of malignancy including squamous carcinoma of cervix and skin. there is growing evidence that human papillomavirus (hpv) has a part to play in malignant transformation at these sites. we have previously identified hpv dna in the skin and genital lesions of such patients by dot and southern blotting. in situ hybridization studies, using biotinylated dna probes for hpv 4, 5 and 8 in skin lesions and 6, 11, 16 and 18 in genital lesions, were pe ...19902157656
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