Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| the incidence of vertical transmission of hepatitis c virus. | this study was undertaken to clarify the incidence of the vertical transmission of hepatitis c virus (hcv). during the third trimester, 2015 pregnant women were examined as to anti-hcv antibodies. anti-hcv antibody seropositive women were examined for hcv-rna in peripheral blood at labor and in breast milk. their offspring were also examined for hcv-rna in umbilical cord blood and peripheral blood one week after birth and during subsequent outpatient visits. the following results were obtained: ... | 1993 | 7512756 |
| markers of hepatitis c virus infection in sardinian blood donors: relationship with alanine aminotransferase levels. | serum samples from 1,765 consecutive sardinian blood donors, negative for hepatitis b surface antigen (hbsag) and for antibodies to human immunodeficiency virus (hiv) (anti-hiv), were evaluated for the presence of antibodies to hepatitis c virus (anti-hcv) by second-generation elisa. anti-hcv was detected in 25 (1.45%) of the 1,765 donors examined. anti-hcv was found in 15 of the 1,690 (0.9%) donors with normal alanine aminotransferase (alt) and in 10 of the 75 (13%) donors with elevated alt (p ... | 1993 | 7508972 |
| prevalence of antibodies to hepatitis c virus in populations at low and high risk for sexually transmitted diseases in rio de janeiro. | in order to investigate the sexual transmission of the hepatitis c virus (hcv), the prevalence of specific antibodies in populations at high and low risk for sexually transmitted diseases (stds) was evaluated. the population at low risk for stds was composed of persons who voluntarily donated blood at the hospital universitário clementino fraga filho (hucff) between july and november, 1990 (n = 2494). the population at high risk for stds was drawn from an ongoing study on the natural history of ... | 1993 | 7509022 |
| serum antibodies to viral pathogens and toxoplasma gondii in hiv-infected individuals. | sera from 38 hiv-infected individuals were examined longitudinally for antibodies to viruses that may increase morbidity in hiv infection, as well as commensal viruses and toxoplasma gondii. htlv infection was seen in norway for the first time as four patients had antibodies to htlv-ii and one had antibodies to htlv-i. antibodies to hepatitis b virus (hbv) were found in 47.2%, while 21.6% of the patients had antibodies to hepatitis c virus (hcv). there was no evidence of acquisition of hbv or hv ... | 1993 | 7509159 |
| hepatitis c virus infection among sexually promiscuous groups and the heterosexual partners of hepatitis c virus infected index cases. | to define the role of sexual transmission in the spread of hepatitis c virus (hcv) infection, a seroprevalence study of antibodies against hcv was performed in populations at high risk for sexually transmitted diseases. subjects included 310 female prostitutes, 88 clients of prostitutes, 168 homosexual men and 147 stable heterosexual partners of index cases reactive for anti-hcv (98 of whom were partners of drug addicts coinfected with hcv and human immunodeficiency virus [hiv]). all subjects de ... | 1993 | 7509282 |
| [anti-hcv in patients without risk factors for hepatitis c. prospective study in 200 patients]. | using a second generation enzyme immunoassay (elisa) for the detection of antibodies against hepatitis c virus (hcv), we investigated the frequency of antibodies anti-hcv and the alanine aminotransferase (alt) plasma levels of 200 patients without history of viral hepatitis, liver diseases, blood transfusions, intravenous drugs abuse, homosexuality, hemodialysis, infection by human immunodeficiency virus (hiv), hepatitis b virus (hbv), nor workers of health services. there plasma samples (1.5%), ... | 1993 | 7509301 |
| [post-transfusional hepatitis due to hepatitis virus c]. | cloned even before observed, c virus hepatitis seems to live out its natural life backwards. the c virus has begun to confide a part of its secrets to molecular biology. today's scientific data demonstrate that, for the most part, occurrences of post-transfusional hepatitis (pth), which are neither a nor b, are the c virus. in order to lower the risk of blood transmission of the c virus, a systematic screening of hcv antibodies has been mandatory in belgium since 1 july 1991. epidemiological dat ... | 1993 | 7522079 |
| chronic hepatitis in haemophilia. | chronic hepatitis affects almost all haemophiliacs treated with non-virally inactivated clotting factor concentrates. the virus responsible is hepatitis c (hcv) and most patients have non-neutralising antibodies with circulating virus. although the majority also have evidence of past infection with hepatitis b, less than 5% are chronic carriers of hbsag. chronic hepatitis c can be associated with severe and progressive liver disease but the development of complications is slow. treatment with re ... | 1993 | 7510563 |
| amplification of the c-myc gene in human hepatocellular carcinoma: biologic significance. | to elucidate the prevalence and biologic significance of the c-myc gene in human hepatocellular carcinoma (hcc), dna samples were taken from the paired tumorous and nontumorous tissues of 77 cases of resected primary hcc and were analyzed by southern blot hybridization. we demonstrated modest, but significant c-myc amplification (group a) in 28 (36.4%) of the cases: 1.6- to 2.0-fold in 18, 2.1- to 3.0-fold in four, and > 3.0-fold in six. compared to hcc without c-myc amplification (group b), gro ... | 1993 | 7511953 |
| a multicenter study of viral hepatitis in a united states hemophilic population. | hemophilia a and b patients seen at nine us regional treatment centers were tested for serologic markers of hepatitis b virus (hbv), hepatitis c virus (hcv), and hepatitis delta virus (hdv) during 1987 and 1988. because human immunodeficiency virus (hiv) infection, a potentially confounding variable, was present in 53% of the group, the population was divided by hiv status for analysis purposes. in the hiv-positive group (n = 382), less than 1% had not been infected with hbv, hcv, or hdv, wherea ... | 1993 | 7678517 |
| expression and identification of hepatitis c virus polyprotein cleavage products. | hepatitis c virus (hcv) is the major cause of transfusion-acquired non-a, non-b hepatitis. hcv is an enveloped positive-sense rna virus which has been classified as a new genus in the flavivirus family. like the other two genera in this family, the flaviviruses and the pestiviruses, hcv polypeptides appear to be produced by translation of a long open reading frame and subsequent proteolytic processing of this polyprotein. in this study, a cdna clone encompassing the long open reading frame of th ... | 1993 | 7679746 |
| an improved method for the detection of hepatitis c virus rna in plasma utilizing heminested primers and internal control rna. | the majority of transfusion-associated, non-a, non-b hepatitis cases are caused by hepatitis c virus (hcv), a positive-stranded rna virus. although high titers of hcv in clinical specimens have been reported, in some cases extremely low titers of virus are not uncommon. therefore, an extremely sensitive and reliable assay is required to determine viremia and replication of hcv accurately. we report here the systematic investigation of factors influencing the detection of hcv rna by a reverse tra ... | 1993 | 7680265 |
| variability in the expression of a beta 2-microglobulin epitope on hepatocytes in chronic type c hepatitis on treatment with interferon. | cytotoxic cd8+ t lymphocytes recognize viral antigens in the context of human leukocyte antigen class i molecule coexpression by target cells. analysis of beta 2-microglobulin reactivity is useful in evaluating changes in human leukocyte antigen class i antigen distribution. in this study we analyzed liver biopsy specimens obtained from 15 patients with chronic active hepatitis type c who underwent a clinical trial with recombinant interferon-alpha 2b. we comparatively studied by immunohistochem ... | 1993 | 7680330 |
| significance of antibody to the host cellular gene derived epitope gor in chronic hepatitis c virus infection. | the humoral response to the host cellular gene-derived epitope gor (anti-gor) was reported to be associated with chronic hepatitis c virus (hcv) infection. to determine the prevalence and clinical significance of anti-gor, sera from 31 patients (m/f, 19/12, age 30-72) with chronic hcv infection (anti-hcv+ in 30, hcv-rna+ by pcr in 31) were tested for anti-gor by enzyme immunoassay. results were correlated with clinical, biochemical and histological features, and the subsequent response to interf ... | 1993 | 7680364 |
| hepatitis c virus infection and progression of infection due to human immunodeficiency virus. | 1993 | 7680584 | |
| lack of mother-to-infant transmission of hepatitis c virus in human immunodeficiency virus-seronegative women: a prospective study with hepatitis c virus rna testing. | the published risk of mother-to-infant transmission of hepatitis c virus varies according to the population studied and the tests used. in a prospective study we used the polymerase chain reaction to assess the risk of vertical transmission of hepatitis c virus in an unselected population of women uninfected by human immunodeficiency virus. hepatitis c virus antibodies were sought with a second-generation enzyme-linked immunosorbent assay in 2,367 consecutive pregnant women. forty-one were posit ... | 1993 | 7684017 |
| characterization of anti-liver-kidney microsome antibody (anti-lkm1) from hepatitis c virus-positive and -negative sera. | hepatitis c virus-related antibodies were found in sera positive for antibodies to liver/kidney microsome antibody, usually considered a marker of autoimmune hepatitis. the aim of this study was to analyze the specificity of this autoantibody in sera from patients with and without hepatitis c virus infection. | 1993 | 7684716 |
| improved detection of antibodies to hepatitis c virus by the second-generation assay in patients with chronic non-a, non-b liver disease. | serum samples from 337 japanese patients with chronic non-a, non-b liver disease were tested for antibodies to hepatitis c virus (hcv) by means of first-generation (c100-3; anti-c100) and second-generation (phcv-34, phcv-31, c100-3; anti-hcv ii) enzyme linked immunosorbent assays (elisa) and for antibody to the gor epitope (anti-gor) also by elisa. anti-hcv ii was detected in 314 (93.2%), anti-c100 in 247 (81.3%) and anti-gor in 211 (62.6%) samples. thus, anti-hcv ii was more sensitive in detect ... | 1993 | 7685043 |
| recombinant human interferon alfa-2b treatment for acute non-a, non-b hepatitis. | to assess the safety and possible efficacy of recombinant human interferon alfa-2b in preventing the development of chronic hepatitis, 24 adults (eight men, 16 women) with acute non-a, non-b (nanb) hepatitis were recruited to a pilot study. half of the cases were parenterally transmitted and half were community acquired. twelve patients received 3 million units (mu) interferon three times weekly subcutaneously for six weeks and the remaining 12 patients received no treatment. anti-hepatitis c vi ... | 1993 | 7686113 |
| a multivariate analysis of risk factors for hepatocellular carcinogenesis: a prospective observation of 795 patients with viral and alcoholic cirrhosis. | to elucidate the appearance rates of hepatocellular carcinoma in cirrhosis and to assess the risk factors for hepatocellular carcinogenesis, we prospectively studied 795 consecutive patients with viral or alcoholic cirrhosis for 2 to 17 yr (median of 5.8 yr). during the observation period, hepatocellular carcinoma developed in 221 patients. cumulative appearance rates of hepatocellular carcinoma were 19.4%, 44.3% and 58.2% at the end of the fifth, tenth and fifteenth years, respectively. when cl ... | 1993 | 7686879 |
| heterosexually acquired human immunodeficiency virus infection and the united states blood supply: considerations for screening of potential blood donors. hiv blood donor study group. | the impact of heterosexual transmission of the human immunodeficiency virus (hiv) on the united states blood supply was assessed, and deferral criteria that may exclude potential donors who are at high risk for heterosexually acquired hiv infection were evaluated. interviews were conducted with 508 hiv-seropositive blood donors from may 1, 1988, to august 31, 1989 (phase 1), and with 472 donors from january 1, 1990, to may 31, 1991 (phase 2), at 20 blood centers. from phase 1 to phase 2, the ove ... | 1993 | 7687391 |
| the presence of hepatitis c virus (hcv) antibody in human immunodeficiency virus-positive hemophilic men undergoing hcv "seroreversion". | hepatitis c virus (hcv) is a major cause of transfusion-induced chronic liver disease in hemophiliacs, with 70% to 90% being anti-hcv positive. seroreversion or loss of antibody response to hcv has been observed in a small proportion of human immunodeficiency virus-positive [hiv(+)] anti-hcv(+) hemophilic men. despite the seroreversion to an anti-hcv-negative state, such patients continue to show serum alanine aminotransferase (alt) elevations and biopsy evidence of cirrhosis and/or chronic acti ... | 1993 | 7687887 |
| high prevalence of thyroid autoantibodies in a prospective series of patients with chronic hepatitis c before interferon therapy. | after describing two cases of hashimoto's thyroiditis associated with chronic hepatitis c, we set up a prospective study to assess the prevalence of thyroid autoantibodies (thyroglobulin and thyroid microsomal autoantibodies) in 72 chronic hepatitis c patients (43 men and 29 women; mean age = 51 +/- 2.1 yr) before interferon therapy admitted between january and december 1991 to our liver unit. thyroid autoantibodies were systematically assayed in 60 chronic hbsag-positive patients (34 men and 26 ... | 1993 | 7687977 |
| post-transfusional anti-hcv-negative, non-a, non-b hepatitis. (i) a prospective clinical and epidemiological survey. | despite the identification of hepatitis c virus (hcv) and the detection of anti-hcv antibodies in the serum of infected individuals, a sizeable proportion of patients who develop transfusion-associated acute non-a, non-b hepatitis following surgery do not develop anti-hcv antibodies. the cause of this disease remains unknown. to assess the role of homologous blood transfusion in anti-hcv-positive and -negative, non-a, non-b hepatitis following surgery, patients receiving homologous blood, autolo ... | 1993 | 7688012 |
| post-transfusional anti-hcv-negative non-a non-b hepatitis (ii) serological and polymerase chain reaction analysis for hepatitis c and hepatitis b viruses. | hepatitis c virus (hcv) is a major etilogical agent of post-transfusional and sporadic acute and chronic hepatitis in various geographical areas. however, anti-hcv seroconversion was uncommon in a recent study of patients with post-transfusional hepatitis in paris, france (n. asar et al., companion paper). the aim of the present study was to detect viral markers, in particular hcv rna and hepatitis b virus (hbv) dna, in these patients. a combination of second-generation assays for anti-hcv antib ... | 1993 | 7688013 |
| a sensitive serodiagnosis of hepatitis c virus (hcv) infection with two non-fused peptides: comparison of antibody responses detected with a newly developed assay and a commercial second-generation test. | an enzyme-linked immunosorbent assay (elisa) was developed for the detection of anti-hcv antibody. we assayed for antibodies against either oligopeptide (s29-1) deduced from the nucleocapsid gene or the product of nonstructural region (ns3) synthesized in a recombinant escherichia coli (s4). to reduce false-positive results induced by non-specific binding of antibodies with a carrier protein and to increase the sensitivity of an immunoassay, non-fused s4 peptide was prepared by the recombinant d ... | 1993 | 7688847 |
| hepatitis c virus infection in patients infected with the human immunodeficiency virus. | the prevalence and characteristics of hepatitis c virus (hcv) infection in 226 patients who were seropositive for human immunodeficiency virus (hiv) were determined. antibody to hcv (anti-hcv) was detected by enzyme immunoassay (eia), and positive results were confirmed by a neutralization eia or recombinant immunoblot assay. the prevalence of anti-hcv was 8%. intravenous drug use was the most common risk factor for hcv infection (61.1% of patients), and 52.4% of intravenous drug users were sero ... | 1993 | 7688986 |
| low risk of sexual transmission of hepatitis c virus. | sexual transmission of hepatitis c virus (hcv) was studied between 104 anti-hcv positive index cases (99 haemophilic men, five women) who have attended the oxford haemophilia centre and 104 (98 female, 6 male) longstanding sexual partners. ninety-one percent of the index cases were hcv rna positive by the polymerase chain reaction (pcr) and 56% were anti-human immunodeficiency virus (hiv) positive. three (2.9%) sexual partners (each a female partner of a different hcv rna positive haemophilic ma ... | 1993 | 7689092 |
| b-cell epitopes on the hepatitis c virus nucleocapsid protein determined by human monospecific antibodies. | four monospecific antibodies against the hepatitis c virus nucleocapsid protein, which was expressed by recombinant baculovirus, were obtained by epstein-barr virus transformation of b cells from three patients with chronic hepatitis c virus infection. one of these antibodies was igg and the other three were igm. their specificities were characterized initially by enzyme-linked immunosorbent assay and immunoblotting against hepatitis c virus proteins expressed by six recombinant baculoviruses wi ... | 1993 | 7689529 |
| htlv-i, hiv-i, and hepatitis b and c viruses in western province, papua new guinea: a serological survey. | seven hundred and twenty-three serum samples from individuals in 13 gidra-speaking villages in western province, papua new guinea were tested for evidence of infection with human t-lymphotropic virus type i (htlv-i), human immunodeficiency virus type i (hiv-i), hepatitis b virus (hbv) and hepatitis c virus (hcv). no samples were positive for antibodies to hiv-i. antibodies to htlv-i were found in 13 samples (1.8%), hbv surface antigens (hbsag) were found in 86 samples (11.9%), and antibodies to ... | 1993 | 7690354 |
| hepatitis c virus in pregnancy: seroprevalence and risk factors for infection. | our purpose was to define the prevalence of antibodies to hepatitis c virus among inner-city prenatal patients. we also sought to examine both the reliability of traditional hepatitis risk factors to predict evidence of infection among these women and the incidence of coinfection in this population with other blood-borne and sexually transmissible agents. | 1993 | 7690523 |
| properties of a human monoclonal antibody specific for the ns4 region of hepatitis c virus. | a human monoclonal igg1(kappa) antibody (hmab) specific for a sequential epitope comprised within the 5-1-1 fragment of the ns4 region of hepatitis c virus (hcv) has been recently generated (1). in this study, b-cell clone supernatant containing the hmab was purified by passage over a protein a affinity column. preincubation with synthetic oligopeptides containing the epitope recognized by the hmab resulted in complete inhibition of binding to the whole recombinant protein, attesting to its spec ... | 1993 | 7691925 |
| hepatitis c virus-specific ctl responses in the liver of chimpanzees with acute and chronic hepatitis c. | hepatitis c virus (hcv)-specific ctl responses were evaluated in two chimpanzees (pan troglodytes) during the acute and chronic phases of hcv infection. cd8+ t lymphocytes were isolated from liver tissue homogenates using anti-cd8 antibody-coated magnetic beads and then stimulated with anti-cd3 antibodies, il-2, and irradiated human pbmc using limiting dilution culture conditions. hcv-specific cytotoxic activity of expanding cd8+ cell lines was assessed against autologous lymphoblastoid cell lin ... | 1993 | 7691940 |
| high prevalence of antibodies to hepatitis c virus in three haemodialysis centres in south-western poland. | we assessed the prevalence of anti-hepatitis c virus (anti-hcv) antibodies and markers of hepatitis b virus (hbv) infection in patients of three haemodialysis centres before initiating anti-hbv vaccinations. of the 94 patients, 39 (41.5%) were anti-hcv positive (+) and 81 (86.2%) were anti-hepatitis b core antigen (hbc) positive. there was a high rate of anti-hbc positivity among anti-hcv (+) patients (92.3%), although the presence of anti-hcv and anti-hbc antibodies were not significantly relat ... | 1993 | 7692355 |
| prevalence of anti-hepatitis c virus in the blood donor population of rio de janeiro. | the prevalence of hepatitis c virus (hcv) antibodies in 2,557 asymptomatic volunteer brazilian blood donors is reported. using the line immunoassay (inno-lia) as a confirmatory test on elisa anti-hcv-positive reacting sera, a prevalence rate of 2.7% for anti-hcv positivity was found. by comparison, prevalences of 1.6% for hepatitis b surface antigen, 0.9% for treponema pallidum, 0.4% for human immunodeficiency virus and 0.04% for trypanosoma cruzi were observed. only 57% of the hcv-positive dono ... | 1993 | 7692672 |
| low prevalence of hepatitis c virus infection in patients with auto-immune hepatitis type 1. | hepatitis c virus (hcv) antibodies were measured in 28 patients with auto-immune hepatitis type 1 using six different assay kits, three for c100-3 antibody and three for second generation hcv antibody, and two confirmatory tests to determine the prevalence of hcv infection in auto-immune hepatitis. these patients were confirmed to have human leucocyte antigen dr 4 or 2 which is susceptible to auto-immune hepatitis in japanese. of the 28 patients, four (14.3%) were positive for hcv antibody in al ... | 1993 | 7693009 |
| hepatitis c virus: guidance on the risks and current management of occupational exposure. phls hepatitis subcommittee. | hepatitis c virus (hcv) transmission after percutaneous exposure to blood at work has been documented. follow up of health care workers who report significant percutaneous or mucocutaneous exposures to sources that are known to be, or that might be, hcv-infected should include storage of serum from the source (if identifiable) and anti-hcv testing of the blood specimen obtained from the health care worker at six months post-exposure. routine testing of the health care worker's initial post-expos ... | 1993 | 7693137 |
| [recombinant antibodies in serologic diagnosis]. | in this paper we discuss the value of a new methodology for the production of monoclonal antibodies by recombinant techniques. this approach is especially useful for human monoclonal antibodies due to instability of human antibody-producing cell lines. as an example, we present a recombinant human antibody to the hepatitis c virus (hcv) core protein. | 1993 | 7693244 |
| hla b44-restricted cytotoxic t lymphocytes recognizing an epitope on hepatitis c virus nucleocapsid protein. | cytotoxic t lymphocytes have been reported to be involved in the immune clearance of virus-infected cells and in the pathogenesis of viral infection. we studied the cytotoxic t lymphocyte response to the putative nucleocapsid protein of hepatitis c virus in patients with chronic hepatitis c. cytotoxic t lymphocytes specific for hepatitis c virus nucleocapsid protein were generated from peripheral blood lymphocytes by means of repeated stimulation with a synthetic hepatitis c virus nucleocapsid p ... | 1993 | 7693568 |
| immunological abnormalities in hiv-free haemophiliacs. | since some haemophiliacs manifest profound immunodeficiency with no evidence of human immunodeficiency virus type 1 (hiv) infection, we measured the circulating immune complex (cic) level in sera obtained from haemophiliacs and addressed the question of whether viral infection is associated directly or indirectly with enhanced cic production. while more than 90% of hiv-positive individuals had a high level of cic, around 60% of seronegative ones also showed cic levels comparable to those of sero ... | 1993 | 7707343 |
| risk for hepatitis c virus (hcv) and human immunodeficiency virus (hiv) infections among prostitutes. | 1993 | 7721298 | |
| [viral markers of acute hepatitis: a, b, c, d, and e in dakar. october 92 - october 93]. | inside of 95 patients presented in hospital with presumed hepatitis: 77 were recruted with liver cytolysis (amino-transferases at > 80 ui/ml) and included in this study. study of serologic viral markers (a, b, c, d and e type) permited to prove viral acute hepatitis infection and 49 patients were recruted inside the 77 cytolytic cases. inside these 49 cases: 44% presented enteritic contamination with hav/hev markers, 36% with hbv markers: hbs/hbc, 6% with hbs/hbe markers, 10% with hdv marker, 4% ... | 1993 | 7758379 |
| hepatitis in pregnancy. acog technical bulletin number 174--november 1992. | hepatitis a is an uncommon complication of pregnancy and is not associated with perinatal transmission. in contrast, hepatitis b virus infection is more common and clearly poses a serious risk to the household contacts and neonates of infected mothers. accordingly, all pregnant women should be tested for hepatitis b virus. universal vaccination of all neonates with hepatitis b vaccine is now recommended. infants delivered to seropositive mothers also should receive hbig immediately after birth. ... | 1993 | 7901073 |
| coexpression of intercellular adhesion molecule-1 and class i major histocompatibility complex antigens on hepatocyte membrane in chronic viral hepatitis. | to evaluate the role of hepatocyte expression of leucocyte adhesion molecules and major histocompatibility complex (mhc) antigens in the pathogenesis of chronic viral hepatitis. | 1993 | 7902850 |
| [the incidence of detecting hepatitis c viral antibodies in different age groups of the population in northeastern ukraine]. | examinations for the presence of antibodies to hepatitis c virus (anti-c100-3) were carried out in 1143 human subjects, residents of the town of sumy, ukraine (681 blood donors, 176 school children, 286 subjects hospitalized for abortions and for treatment of acute traumas). anti-hcv were determined by enzyme immunoassay using a commercial test system "abbott" of the first generation, with confirmation of the positive findings by neutralization test. anti-hcv were found in 2.3% free-giving blood ... | 1993 | 8073754 |
| hepatitis c virus rna in saliva and breastmilk of hepatitis c carrier mothers. | 1993 | 8094800 | |
| natural history of hepatitis c virus infection in multitransfused hemophiliacs: effect of coinfection with human immunodeficiency virus. the multicenter hemophilia cohort study. | the objective of this prospective cohort study was to describe the natural history of hepatitis c virus (hcv) infection and the effect of human immunodeficiency virus (hiv) on the clinical manifestations of hcv liver disease. two hundred twenty-three hemophiliacs were followed in a comprehensive care setting with periodic clinical and laboratory evaluations. dates of hiv seroconversion were determined retrospectively from frozen sera. hcv assays were performed by a "second generation" four-antig ... | 1993 | 8098752 |
| p53 gene mutations and 17p allelic deletions in hepatocellular carcinoma from japan. | p53 gene mutations at codon 249 have been reported in hepatocellular carcinoma (hcc) from china and south africa, a phenomenon shown to be closely associated with food contamination by aflatoxin. there have been few reports, however, in regard to p53 gene mutations in hcc from other geographic areas. | 1993 | 8100480 |
| intercellular adhesion molecule-1 concentration in sera of patients with acute and chronic liver disease: relationship to disease activity and cirrhosis. | to study the influence of chronic hepatitis on intercellular adhesion molecule-1 serum concentration, we measured intercellular adhesion molecule-1 in the serum of 84 patients with chronic liver disease (17 chronic persistent hepatitis, 42 chronic active hepatitis and 25 active cirrhosis) caused by hepatitis b virus (n = 46), hepatitis c virus (n = 10) and autoimmunity (n = 28). furthermore, 20 patients with acute viral hepatitis (16 hepatitis b virus and 4 hepatitis a virus) and 6 patients with ... | 1993 | 8104856 |
| density heterogeneities of hepatitis c virus in human sera due to the binding of beta-lipoproteins and immunoglobulins. | heterogeneities in the density of hepatitis c virus rna-carrying material (hcv-rna-cm) found in human sera (1.03-1.20 g/cm3) are attributed to the binding of low-density lipoproteins and/or of igg. in some sera hcv-rna-cm seems to be nearly totally bound to beta-lipoproteins and cannot be precipitated by anti-igg (gamma); in others more than 95% of hcv-rna-cm is bound to igg and cannot be precipitated by anti-beta-lipoprotein. furthermore, there are sera from which hcv-rna-cm can be completely b ... | 1993 | 8121333 |
| treatment of six patients with chronic active hcv hepatitis, with low dose natural human interferon alpha administered orally. | following the widely accepted therapeutic standard of treatment of hcv infection with parenteral interferon alpha, and encouraged by the author's won good experience with orally administered natural human interferon alpha in low doses (leuhuifn alpha (ldou)), applied to chronic active hbv hepatitis patients, this form of interferon was given to six randomly selected hcv infected patients (2 women, 4 men) aged 34-62 years. the diagnosis was made based on a clinical and histological evaluation and ... | 1993 | 8129569 |
| theoretical and technical concerns in inactivation/elimination of viruses in plasma derivatives. | to know the virus eliminating/inactivating capacity of the manufacturing process of a plasma protein, it is essential to analyse it by adding virus to the source material or to different materials obtained at various stages of the manufacturing procedure and then to determine the elimination/inactivation of this virus. to carry out such experiments properly, three prerequisites have to be fulfilled: (i) the manufacturing procedure must be scaled down as exactly as possible; (ii) relevant test vi ... | 1993 | 8174794 |
| viral safety of solvent-detergent treated blood products. | laboratory research commencing in 1982 led to licensing in the united states in 1985 of a solvent/detergent (sd)-treated anti-haemophilic factor (ahf) concentrate. licensing was based on (a) studies demonstrating the inactivation of several marker viruses [vesicular stomatitis virus (vsv), sindbis virus, sendai virus], human immunodeficiency virus (hiv), hepatitis b virus (hbv), and non-a, non-b hepatitis virus [nanbhv; now known to be principally hepatitis c virus (hcv)] added to ahf just befor ... | 1993 | 8174797 |
| inactivation of hiv, hbv, hcv related viruses and other viruses in human plasma derivatives by pasteurisation. | laboratory studies were made to verify the capacity and efficacy of the pasteurisation step (heat treatment at 60 degrees c for 10 hours in stabilized aqueous solution) in inactivating various pathogenic viruses (e.g. hiv, hbv, hcv related viruses, hav, hsv, poliovirus). these experiments proved that pasteurisation effectively inactivates a broad spectrum of viruses with a range of different physicochemical properties. the results, summarized in inactivation kinetics, demonstrate that complete i ... | 1993 | 8174799 |
| viral validation of the manufacturing process of high purity albumin from placentas. | we report on the viral validation of an industrial purification process which is used to manufacture high purity human albumin from frozen placentas. this process has been used without any modification since 1980 except for a progressive increase in production scale to reach a capacity of 19 tons of placentas per day. the extraction purification process includes five alcohol manufacturing steps, some with strict conditions of alcohol concentration, acid ph, temperature and one including chlorofo ... | 1993 | 8174808 |
| hepatitis c, other types of non-b parenteral hepatitis and blood transfusion. | since the implementation of sensitive screening tests for hepatitis b virus infection, the majority of post-transfusion hepatitis has been due to infection with hepatitis c virus (hcv). the recent introduction of tests for antibodies to this virus has demonstrably reduced the incidence of post-transfusion hcv infection. there is some residual post-transfusion and sporadic hepatitis, which may possibly be caused by an as yet undescribed aetiologic agent. however, evidence for or against such an a ... | 1993 | 8174817 |
| validation of virus inactivation and removal for the manufacturing procedure of two immunoglobulins and a 5% serum protein solution treated with beta-propiolactone. | intravenous immunoglobulins and serum protein solutions are manufactured from human plasma pools of healthy, screened donors. a step-by-step validation of virus removal and/or inactivation was performed for the manufacturing process, which includes cold ethanol fractionation, beta-propiolactone (beta-pl) treatment, uv irradiation, thermal inactivation and other chemical and physical purification steps. the total viral clearance factors achieved for the entire manufacturing process were by severa ... | 1993 | 8117439 |
| viral hepatitis in pregnancy. | hepatitis a is usually a benign, self-limited infection. a chronic carrier state does not exist, and perinatal transmission does not occur. hepatitis b may cause chronic infection, and infants delivered to infected mothers are at considerable risk of developing neonatal hepatitis. passive and active immunization with hbig and hbv is highly effective in preventing perinatal transmission. hepatitis d typically occurs as a coinfection or superinfection with hepatitis b. patients infected with both ... | 1993 | 8160022 |
| viral diseases in ethiopia: a review. | ethiopia is endemic for many viral diseases. serosurveys have demonstrated the high prevalence rate of hepatitis b virus. there are also indications of high transmission for hepatitis c, hepatitis e and human immunodeficiency virus (hiv). the population is exposed to poliomyelitis, hepatitis a, measles, rubella and mumps early in life. rotaviral diarrhoea is an important cause of infant morbidity and mortality. vast areas of the country are endemic for yellow fever and rabies. the extent of many ... | 1993 | 8187657 |
| neurotropism of human coronavirus 229e. | the 299e prototype strain of human coronavirus (hcv-229e) has so far been mainly associated with infections of the respiratory tract. in the present study, we show evidence for infection of the central nervous system (cns) by hcv-229e, both in vitro and in vivo. various human cell lines of cns origin were tested for their susceptibility to infection by hcv-229e. production of viral antigens was monitored by indirect immunofluorescence with monoclonal antibodies and infectious progeny virions by ... | 1993 | 8209751 |
| genome organization of porcine epidemic diarrhoea virus. | in order to study the organization of the genome of porcine epidemic diarrhoea virus (pedv), we constructed a cdna library in a phage expression vector by using poly(a) rna from pedv-infected vero cells. an anti-pedv hyperimmune serum was used to probe the library. the first isolated clone mapped within the n gene and was subsequently used for rescreening the library. the selected clones allowed us to establish the sequence of the 3'-most 7.4 kb of the pedv genome. analysis of the cdna sequences ... | 1993 | 8209771 |
| characterization of the human coronavirus 229e (hcv 229e) gene 1. | the sequence of the hcv 229e gene 1 has been determined and compared with the homologous sequences of the murine hepatitis virus and the avian infectious bronchitis virus. the coding sequence of gene 1 is 20,273 nucleotides in length. within this coding region are two large open reading frames, orf 1a (4,086 codons) and orf 1b (2,687 codons) which overlap by 40 nucleotides. in the overlapping region, the genomic rna can be folded into a pseudoknot structure, an element which is known to mediate ... | 1993 | 8209774 |
| replication and multiplication of hepatitis c virus genome in human foetal liver cells. | the ability to obtain primary long-term cultures of human foetal hepatocytes maintaining liver differentiation characteristics in serum-free medium prompted us to test their susceptibility to hepatitis c virus infection. using pcr, we detected the presence of the hcv rna-positive strand in the supernatants and in the cells of the virus-infected hepatocyte cultures, at various times post-infection. evidence of effective virus genome replication and multiplication was also based on the time-depend ... | 1993 | 8210708 |
| the human bone-marrow-derived b-cell line ce, susceptible to hepatitis c virus infection. | the euploid-diploid cell line ce, issuing spontaneously from a normal human bone marrow culture, displays b-cell differentiation and activation markers and is positive (> or = 90% of cells) for epstein-barr nuclear antigens. ce suspensions were inoculated with serum from a patient chronically infected with hepatitis c virus (hcv). after inoculation, the cells were regularly subcultured with a split ratio of 1:2 every 4-6 days. rna extracted as late as 65 days after infection from the inoculated ... | 1993 | 8210709 |
| analysis of hepatitis c virus capsid, e1, and e2/ns1 proteins expressed in insect cells. | the baculovirus/insect cell expression system was used to express the capsid protein and glycoproteins (e1 and e2/ns1) of the hepatitis c virus (hcv). each polypeptide domain was expressed individually using two different constructs varying at their carboxy termini in order to retain or delete hydrophobic domains that may be involved in membrane association. the capsid proteins were transported to the nucleus where they formed a single large crystal-like inclusion. the capsid proteins were phosp ... | 1993 | 8212557 |
| [hepatitis c virus in the aqueous humor]. | since a method for detecting hepatitis c virus (hcv) antibody has been established, many cases of non-a non-b sporadic and post-transfusion hepatitis are now known to have been caused by hcv. it is important to take steps against the nosocomial transmission of hcv as with the hepatitis b virus. sera from patients in our ophthalmological clinic were tested for the presence of hcv antibody during the seven months from oct. 1991 to apr. 1992, utilizing c-100-3 antigen. we then searched for the pres ... | 1993 | 8213365 |
| hepatitis c virus infection in type ii mixed cryoglobulinemia. | 1993 | 8216392 | |
| serological and antigenical findings indicating pestivirus in man. | an epidemiological survey for pestivirus was undertaken in zambia and europe, in view of the recent serological findings obtained by previous studies in europe with humans. collected sera were tested for anti-bovine viral diarrhea virus (bvdv) specific antibodies by iif and western blotting. of those individuals tested (n = 1272), 15.3% showed a seropositive reaction to the bvdv. anti-bvdv antibody prevalence in immuno-depressed patients (e.g. hiv positive) was investigated. a higher prevalence ... | 1993 | 8219813 |
| molecular analysis of the human coronavirus (strain 229e) genome. | the nucleotide sequence of the human coronavirus strain 229e (hcv 229e) has been determined. this article describes the organization of the virus genome, the predicted viral gene products and the mechanisms which regulate viral gene expression. this information provides a basis to investigate the biology and pathogenesis of hcv. | 1993 | 8219814 |
| [synthetic oligopeptide of hepatitis d virus antigen and its clinical application]. | a 27-peptide fragment derived from natural hdag sequence was selected, identified and synthesized. it was used to develop an eia method for detection of anti-hd. the 27-peptide possessed the similar antigenicity with the corresponding fragment of natural hdag, and could also compete with natural hdag for serum anti-hd. high specificity and no cross-reaction were found among the peptide and normal human sera, normal mice sera and sera positive with hav, hbv or hcv antibody alone. 36 serum samples ... | 1993 | 8221244 |
| [chronic mucocutaneous herpes simplex infection. occurrence within the scope of liver-induced immunodeficiency]. | herpes simplex virus (hsv) infections causing severe disease are reported frequently in patients suffering from human immunodeficiency virus (hiv) infection. this disease pattern may also be seen in an immunocompromised disease state with other causes, however, as in the case presented in this paper. an 84-year-old woman had hepatic cirrhosis resulting from chronic hepatitis c virus infection. the woman developed ulcerative lesions in and around her mouth and in the genito-anal region, and these ... | 1993 | 8225977 |
| genetic variability of german hepatitis c virus isolates. | heterogeneity of hepatitis c viral (hcv) genomes of several isolates from different countries has been reported, but there is little information on hcv isolates for the federal republic of germany. therefore, the nucleotide (nt) and deduced amino acid (aa) sequences of interesting parts of the viral genome derived from different human isolates in germany were compared with each other and with the nt and predicted aa sequences of recently published isolates. hcv sequences were obtained by reverse ... | 1993 | 8228920 |
| prevalence of hepatitis b, c, and d virus markers in yemeni patients with chronic liver disease. | a serological survey for hepatitis b, c, and d markers was carried out in the yemen republic. serum samples from 243 pregnant females, 294 male blood donors, and 108 patients with chronic liver disease were examined. hepatitis b surface antigen (hbsag) was found in 18.5% healthy individuals and 24.1% patients with chronic liver disease (p = 0.03). evidence of any marker for hepatitis b virus (hbv) infection was found in 59.8% healthy individuals and 75.9% of patients with chronic liver disease ( ... | 1993 | 8228926 |
| detection of hepatitis c virus genome in human serum by multi-targeted polymerase chain reaction. | a multi-targeted "hemi-nested" pcr (m-pcr) assay in which the primer pairs derived from the 5' non-coding (5'nc) and the nonstructural protein 3 (ns3) regions of hcv genome were concurrently used for amplification in order to compare the sensitivity and specificity of polymerase chain reaction (pcr) with different primer pairs in detecting hepatitis c virus (hcv) genome. sera from patients with virus-associated liver diseases were examined for the presence of hcv rna by the m-pcr method followin ... | 1993 | 8228939 |
| prospective study of the evaluation of hepatitis c virus infectivity in a high-purity, solvent/detergent-treated factor viii concentrate: parallel evaluation of other markers for lipid-enveloped and non-lipid-enveloped viruses. the ad hoc study group of the fondazione dell'emofilia. | this prospective study was carried out with the aim of evaluating the efficacy of solvent/detergent inactivation of the hepatitis c virus (hcv) as applied to a chromatographic factor viii concentrate. in parallel, the markers for other viruses, either lipid-enveloped (human immunodeficiency virus types 1 and 2 [hiv-1 and -2] and hepatitis b virus [hbv]) or non-lipid-enveloped viruses (such as b19 parvovirus and hepatitis a virus [hav]) were evaluated. the study included 14 hemophilia centers, wh ... | 1993 | 8236421 |
| high rate of infection with the human t-cell leukemia retrovirus type ii in four indian populations of argentina. | sera from 215 non-drug-injecting toba and mataco-mataguayo pure indians belonging to four communities in northern argentina were examined using assays that allow differentiation between reactivities due to type-specific antigens of the human t-cell leukemia/lymphoma virus (htlv). three of these populations have very little contact with non-indian groups and reside in remote, isolated areas. htlv-ii type-specific seroreactivity was present in 24 (13.7%) of the 175 indians older than 13 years of a ... | 1993 | 8249280 |
| studies on transmission of hepatitis c virus from mother-to-child in the perinatal period. | to elucidate whether breast milk, vaginal discharge and contamination with maternal blood at birth are possible routes of mother-to-child transmission of hepatitis c virus (hcv), we examined hcv rna in the cord and peripheral blood of infants, and in the blood, vaginal discharge, and breast milk of anti-hcv seropositive mothers. from july 1991 to july 1992, we studied 20 healthy pregnant women, who were seropositive with the ortho anti-hcv eia, and their infants. using a sensitive nested polymer ... | 1993 | 8250598 |
| quantitative evaluation of hepatitis c virus rna in patients with concurrent human immunodeficiency virus infections. | quantitation of the hepatitis c virus (hcv) provides a powerful epidemiologic and therapeutic method for the evaluation of infected patients. in this study semiquantitative reverse transcriptase polymerase chain reaction (pcr) is compared with a new branched dna signal amplification methodology. samples from hcv-infected patients as well as from human immunodeficiency virus-infected patients were evaluated. reverse transcriptase pcr correlated well with the branched dna assay (r = 0.7036, p < 0. ... | 1993 | 8253965 |
| [hemophilia a complicated with hepatocellular carcinoma]. | a 60-year-old hemophiliac man complaining of abdominal discomfort was admitted on suspicion of hepatocellular carcinoma (hcc) by ultrasonography (us). when he was 22-year-old, blood transfusion was given for intra-abdominal hematoma due to an external wound and a diagnosis of moderate hemophilia a and non-a non-b type chronic hepatitis (later, c type chronic hepatitis) was made at the age of 40. after admission, hcc in the s6 segment of the liver was diagnosed by us, computerized tomography, mag ... | 1993 | 8254912 |
| immunohistochemical detection of the ns4 antigen of hepatitis c virus and its relation to histopathology. | the immunohistochemical localization of the hepatitis c virus (hcv) nonstructural antigen 4 (ns4) was investigated in formalin-fixed human liver biopsy samples taken from 10 patients who were anti-hcv positive. ns4 was detected within the cytoplasm of hepatocytes in all hcv-positive patients studied, but not in the mononuclear cell infiltrates, bile duct epithelium, or endothelial cells. a high proportion of hepatocytes appeared positive, but the staining intensity was variable. after a coded hi ... | 1993 | 8256849 |
| [molecular cloning and sequencing of hepatitis c virus gene from human blood]. | to clone and characterize hepatitis c virus strain in china, we extracted rna from the pooled plasma of persons with hcv infection in hunan, china. hcv gene was amplified and cloned by rt-pcr and gene recombinant techniques. a total of 2,307 nucleotides of the complementary dna clones of hcv (hcv-hun), including the clone of 5' noncoding region (311bp), core region (340bp), envelope region (397bp), ns1 region (634bp), ns3 region (245bp), and ns5 region (380bp) were isolated and identified. the h ... | 1993 | 8258100 |
| adverse effects of intravenous immunoglobulin. | the range of diseases in which intravenous immunoglobulin (ivig) is effective has expanded significantly since its initial use in primary antibody deficiency. there are at present at least 17 preparations of ivig in use worldwide with similar profiles of adverse effects. infusion-related effects range in severity. mild adverse reactions (headache, flushing, low backache, nausea, wheezing) are often associated with a fast infusion rate, and respond rapidly on slowing the infusion. very rare episo ... | 1993 | 8260119 |
| multiple viral infections in hiv-infected children with chronically-evolving hepatitis. | hepatic involvement was investigated in 31 children with perinatal hiv-1 infection, who were followed for 2-82 months (mean 30.5). liver disease, as revealed by increased aminotransferase levels, liver biopsy or necroscopy, was diagnosed in 18 children (58%), of which 7 (22.5%) had acute hepatitis and 11 (35.5%) showed chronic liver disease. overall, 40 persistently active or recurrent viral infections, as demonstrated by positive culture and/or detection of serum dna, specific igm, iga and high ... | 1993 | 8260869 |
| susceptibility of human liver cell cultures to hepatitis c virus infection. | to develop a cell culture system susceptible to infection by hepatitis c virus (hcv), human fetal hepatocytes, grown in serum-free medium, were inoculated with serum samples from two hcv-infected patients. viral rna sequences were detected by polymerase chain reaction, using primers specific for the 5' noncoding region of hcv, in extracts prepared from the hepatocyte cultures as early as 5 days after inoculation. virus was also released from the infected cells into the medium. the hcv strains co ... | 1993 | 8260875 |
| [a preliminary report on the prevalence of different types of hepatitis in adult patients with acute sporadic hepatitis in beijing area]. | in order to investigate the prevalence of different types of viral hepatitis in beijing area, the markers of viral hepatitis a, b, c and e were detected on 72 adult patients with acute viral hepatitis in the infectious diseases department of our hospital. the results showed that 26.4% of the cases had hav infection, 8.3% hbv infection, 18.1% hcv infection and 31.9% hev infection. it is shown that the prevalence of hev infection in beijing area is high. | 1993 | 8269760 |
| low temperature dependent igg3 subclass antibody with low avidity to c100-3 antigen derived from hepatitis c virus in voluntary blood donors. | the presence of an antibody which reacts strongly at lower temperatures to c100-3 antigen derived from the hepatitis c virus (hcv) was demonstrated in blood donors' sera. these sera reacted with the antigen most strongly at 4 c and most weakly at 37 c and are termed 'cold antibody group'. the cold antibody group reaction was specifically inhibited with soluble c100-3 antigen. cold antibody bound to c100-3 antigen at 4 c was dissociated at 37 c and was isolated in this manner. low temperature-dep ... | 1993 | 8271476 |
| autoimmune responses as assessed by hypergammaglobulinemia and the presence of autoantibodies in patients with chronic hepatitis c. | we investigated autoimmunity, as assessed by hypergammaglobulinemia and the presence of autoantibodies including anti-nuclear antibodies (ana) and anti-liver membrane antibodies (lma), in 149 patients with chronic hepatitis c, 55 patients with chronic hepatitis b and 11 patients with autoimmune hepatitis. there was no significant difference in the incidence of these autoantibodies between chronic hepatitis c and chronic hepatitis b. nine patients with chronic hepatitis c satisfied the serologica ... | 1993 | 8273454 |
| autoantibodies against the human asialoglycoprotein receptor: effects of therapy in autoimmune and virus-induced chronic active hepatitis. | the hepatic asialoglycoprotein receptor (asgpr) was recently identified as a target antigen for both humoral and cellular immune response in inflammatory liver diseases. thereby anti-asgpr autoantibodies directed against human substrate were closely associated with autoimmune chronic active hepatitis. the present study compares the occurrence, titer and immunoglobulin classification of anti-human(h-)-asgpr antibodies in 23 patients with newly diagnosed autoimmune chronic hepatitis before and aft ... | 1993 | 8301043 |
| prevalence of hepatitis virus infections in albanian refugees. | a sample of 393 albanian refugees, including both children and adults, was tested for serological hav, hbv, hdv and hcv markers. a high prevalence of infection with both the hepatitis a and b viruses was found, while hdv and hcv infections were uncommon. the overall prevalence of anti-hav was 96%; it was very high in children 0-10 years, suggesting that hav infection is largely acquired during childhood and that poor ambiental conditions influence the spreading of this viral infection. one or mo ... | 1993 | 8307139 |
| role of screening in prevention and treatment. | since viral hepatitis may be the most common form of chronic viral disease in the world, strenuous attempts are being made to reduce the incidence. to achieve this, strategies are being developed by various national and international bodies involving both the immunisation and screening of certain groups of the population. these strategies are by no means universal, and the value of screening specific groups is the subject of much debate. this paper will address a number of the issues related spe ... | 1993 | 8314491 |
| structural and functional analysis of the surface protein of human coronavirus oc43. | the two surface glycoproteins s and he of human coronavirus oc43 (hcv-oc43) were isolated from the viral membrane and purified. only the s protein was able to agglutinate chicken erythrocytes, indicating that this viral protein is the major hemagglutinin of hcv-oc43. the receptor determinant recognized by this virus on the surface of erythrocytes is n-acetyl-9-o-acetylneuraminic acid (neu5,9ac2) which is also used by bovine coronavirus for attachment to cells. by analyzing erythrocytes containin ... | 1993 | 8317096 |
| occupational exposure to the human immunodeficiency virus and other blood-borne pathogens. a six-year prospective study. | to prospectively study occupational exposures to human immunodeficiency virus (hiv) and other blood-borne pathogens. | 1993 | 8326890 |
| [prevalence of hepatitis c virus and human immunodeficiency virus infection among japanese female prostitutes]. | to investigate the incidence of hepatitis c virus (hcv) and human immunodeficiency virus (hiv) infection in japanese sexual workers, 191 out of 194 female prostitutes working in a certain district of the tokyo metropolitan area were tested for anti-hcv, anti-hepatitis b virus (hbv), anti-hiv and anti-treponema pallidum (tp). they were also interviewed with regard to their past history of blood transfusion, vaccination against hbv, liver diseases and repeated skin piercing treatments such as tatt ... | 1993 | 8329759 |
| nucleotide sequence of the human coronavirus 229e rna polymerase locus. | the nucleotide sequence of the human coronavirus 229e (hcv 229e) rna polymerase gene and the 5' region of the genome has been determined. the polymerase gene is comprised of two large open reading frames, orf1a and orf1b, that contain 4086 and 2687 codons, respectively. orf1b overlaps orf1a by 43 bases in the (-1) reading frame. the in vitro translation of sp6 transcripts which include hcv 229e sequences encompassing the orf1a/orf1b junction show that expression of orf1b can be mediated by ribos ... | 1993 | 8337838 |
| [virus and autoimmunity with emphasis on liver diseases]. | the dualism virus pathogenesis and autoimmune pathogenesis in liver disease is given up increasingly. a survey is given about the development of autoimmunity and virus induction of the autoimmune hepatitis. the hypothesis of virus induction of autoimmune hepatitis put forward by storch 1975 (19) is unproven up to now, but the concept of virus-associated autoimmune hepatitis put forward by storch 1980 (23) is acknowledged today. first of all, antibodies against endoplasmic reticulum (liver and ki ... | 1993 | 8344697 |
| characterization of monoclonal antibodies to bovine enteric coronavirus and antigenic variability among quebec isolates. | twenty monoclonal antibodies (mabs) were prepared against the mebus strain of bovine enteric coronavirus, 14 of them reacting with the peplomeric s (gp 100) glycoprotein. competition binding assays allowed the definition of at least 4 distinct antigenic domains for the s glycoprotein, designated as a, b, c, and d; epitopes associated to neutralizing activity being located in sites a, b, and c. one mab directed to the hemagglutinin he (gp 140/gp 65) glycoprotein inhibited the hemagglutinating act ... | 1993 | 8347084 |
| cryoglobulinemia related to hepatitis c virus infection in patients with human immunodeficiency virus infection. | 1993 | 8353996 | |
| prevalence of hepatitis c in a chemically dependent population. | drug abuse is the major risk factor for hepatitis c in the united states. the objective of this study was to determine the prevalence of hepatitis c virus (hcv), to identify risk factors for hcv, and to correlate hcv and liver function in patients presenting for inpatient detoxification of substances of abuse. | 1993 | 8357288 |
| specific detection of anti-hbc antibodies with an enzyme immunoassay using recombinant hbcag and monoclonal antibodies. | an enzyme immunoassay for the detection of total anti-hbc antibodies in undiluted serum samples was developed. this assay utilizes an anti-hbc monoclonal antibody and a recombinant hbc antigen. the results of the clinical validation are now reported. a total of 1,301 sera were tested using both the recombinant total hbc ab eia and a reference assay. the specificity was evaluated on a panel of 573 normal human sera and human sera from subjects with pathological findings unrelated to a hepatitis b ... | 1993 | 8360313 |
| prevalence of human immunodeficiency virus, hepatitis b virus and hepatitis c virus among dialysis patients. the italian multicentric study on nosocomial and occupational risk of blood-borne infections in dialysis. | in april 1991, a cross-sectional study of hiv, hbv and hcv markers among dialysis patients was carried out in 19 italian units in order to evaluate the spread of these bloodborne infections among patients and to evaluate the potential risk for staff who care for them. a total of 2,180 patients were eligible and all consented to be tested. of the 1,347 patients who had not been given hepatitis b vaccine, 67.9% had at least one marker of hbv infection; of these 9.2% were hbsag carriers; conversely ... | 1993 | 8366992 |