Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| quinolone-resistant campylobacter infections: risk factors and clinical consequences. | we integrated data on quinolone and macrolide susceptibility patterns with epidemiologic and typing data from campylobacter jejuni and c. coli infections in two danish counties. the mean duration of illness was longer for 86 patients with quinolone-resistant c. jejuni infections (median 13.2 days) than for 381 patients with quinolone-sensitive c. jejuni infections (median 10.3 days, p = 0.001). foreign travel, eating fresh poultry other than chicken and turkey, and swimming were associated with ... | 2004 | 15207057 |
| adaptation of campylobacter jejuni nctc11168 to high-level colonization of the avian gastrointestinal tract. | the genome sequence of the human pathogen campylobacter jejuni nctc11168 has been determined recently, but studies on colonization and persistence in chickens have been limited due to reports that this strain is a poor colonizer. experimental colonization and persistence studies were carried out with c. jejuni nctc11168 by using 2-week-old light sussex chickens possessing an acquired natural gut flora. after inoculation, nctc11168 initially colonized the intestine poorly. however, after 5 weeks ... | 2004 | 15213117 |
| risk factors for campylobacter spp. infection in senegalese broiler-chicken flocks. | our objective was to identify the risk factors for campylobacter infection in senegalese broiler flocks. seventy broiler farms were studied around dakar from january 2000 to december 2001 around dakar. a questionnaire was administered to the farmers, and samples of fresh droppings were taken to assess the flocks' campylobacter status. about 63% of the flocks were infected by campylobacter spp.; campylobacter jejuni was the most-prevalent species (p < 0.05). an elevated risk of campylobacter infe ... | 2004 | 15219966 |
| direct microscopic observation of viability of campylobacter jejuni on chicken skin treated with selected chemical sanitizing agents. | the objective of this research was to determine the effect of chlorine, acidified sodium chlorite, and peracetic acid treatments on viable campylobacter jejuni located at various depths within follicles or folds of chicken skin. chicken skin was inoculated with c. jejuni transformed with p(c)gfp plasmid (gfp-campylobacter), which also codes for kanamycin resistance. effectiveness of sanitizer treatments was determined by plate count. c. jejuni were also observed on chicken skin by confocal scann ... | 2004 | 15222541 |
| iron acquisition and regulation in campylobacter jejuni. | iron affects the physiology of bacteria in two different ways: as a micronutrient for bacterial growth and as a catalyst for the formation of hydroxyl radicals. in this study, we used dna microarrays to identify the c. jejuni genes that have their transcript abundance affected by iron availability. the transcript levels of 647 genes were affected after the addition of iron to iron-limited c. jejuni cells. several classes of affected genes were revealed within 15 min, including immediate-early re ... | 2004 | 15231804 |
| determination of toxicity of campylobacter jejuni isolated from humans and from poultry carcasses acquired at various stages of production. | the research focused on the determination of the toxicity variation associated with campylobacter jejuni isolated from humans and chickens. | 2004 | 15239701 |
| longitudinal study of campylobacter jejuni bacteriophages and their hosts from broiler chickens. | a longitudinal study of bacteriophages and their hosts was carried out at a broiler house that had been identified as having a population of campylobacter-specific bacteriophages. cloacal and excreta samples were collected from three successive broiler flocks reared in the same barn. campylobacter jejuni was isolated from each flock, whereas bacteriophages could be isolated from flocks 1 and 2 but were not isolated from flock 3. the bacteriophages isolated from flocks 1 and 2 were closely relate ... | 2004 | 15240258 |
| genetic relatedness and quinolone resistance of campylobacter jejuni strains isolated in 2002 in hong kong. | pulsed-field gel electrophoresis fingerprints of 98 campylobacter jejuni isolates from patients (85) and chicken carcasses (13) in hong kong in 2002 demonstrated high genetic diversity. the prevalence of quinolone resistance among the isolates was 85.9%, and replacement of the threonine-86 residue in the gyrase subunit a was the major resistance mechanism. | 2004 | 15243105 |
| occurrence of campylobacter in retail foods in ireland. | a surveillance study was carried out to determine the prevalence of campylobacter in a range of retail foods purchased in three irish cities over a 20-month period between march 2001 and october 2002. in total 2391 food samples were analysed during this period. campylobacter was isolated from 444 raw chicken (49.9%), 33 turkey (37.5%) and 11 duck samples (45.8%). lower isolation rates of 7/221 (3.2%), 10/197 (5.1%) and 31/262 (11.8%) were observed for raw beef, pork and lamb, respectively. one s ... | 2004 | 15282123 |
| some potential sources for transmission of campylobacter jejuni to broiler chickens. | the aim of the study was to determine campylobacter jejuni contamination and prevalence on fomites moving between broiler farms and the processing plant in the period after cleaning and before departure to harvest chickens. in addition, changes in the proportion of contaminated fomites in the course of a day were assessed. | 2004 | 15287870 |
| species identification by genotyping and determination of antibiotic resistance in campylobacter jejuni and campylobacter coli from humans and chickens in sweden. | campylobacter is today the most common cause of human bacterial enteritis in sweden, as well as in most other industrialized countries. common sources of infection are undercooked chicken meat, unpasteurized milk and contaminated drinking water. one aim with our present study was to identify the species campylobacter jejuni and campylobacter coli strains from humans and chickens using a polymerase chain reaction/restriction enzyme analysis (pcr/rea) method, as well as traditional hippurate hydro ... | 2004 | 15364471 |
| use of culture, pcr analysis, and dna microarrays for detection of campylobacter jejuni and campylobacter coli from chicken feces. | a dna microarray for detection of campylobacter spp. was recently developed and applied to detect campylobacter spp. directly from chicken feces. sixty-five pooled chicken cloacal swab samples from 650 individual broiler chickens were included in the study. the results of campylobacter sp. detection obtained with dna microarrays were compared to those obtained by conventional culture and gel electrophoresis. by conventional culture, 60% of the samples were positive for either campylobacter jejun ... | 2004 | 15364980 |
| a european survey of antimicrobial susceptibility among zoonotic and commensal bacteria isolated from food-producing animals. | to study antimicrobial resistance in zoonotic bacteria isolated from food animals in different countries using uniform methodology. | 2004 | 15375107 |
| nonimmunized egg yolk powder can suppress the colonization of salmonella typhimurium, escherichia coli o157:h7, and campylobacter jejuni in laying hens. | if colonized, poultry shed enteric pathogens in the feces. of those that colonize poultry salmonella spp., campylobacter jejuni (c. jejuni), and escherichia coli (e. coli) o157:h7 are the most concern to the industry. the authors previously discovered that the introduction of 5% dried nonimmunized egg yolk powder in the regular feed could eliminate and prevent salmonella enteritidis intestinal colonization of laying hens. hence, the efficacy of nonimmunized dried egg yolk powder supplement in co ... | 2004 | 15384899 |
| kitchen practices used in handling broiler chickens and survival of campylobacter spp. on cutting surfaces in kampala, uganda. | cross-contamination during food preparation has been identified as an important factor associated with foodborne illnesses. handling practices used during preparation of broiler chickens in 31 fast-food restaurants and 86 semisettled street stands (street vendors) were assessed by use of a standard checklist. these establishments used wood, plastic, or metal cutting surfaces during the preparation of broiler chickens. the survival of campylobacter spp. on kitchen cutting surfaces was determined ... | 2004 | 15453589 |
| flies and campylobacter infection of broiler flocks. | a total of 8.2% of flies caught outside a broiler house in denmark had the potential to transmit campylobacter jejuni to chickens, and hundreds of flies per day passed through the ventilation system into the broiler house. our study suggests that flies may be an important source of campylobacter infection of broiler flocks in summer. | 2004 | 15496257 |
| detection of seven virulence and toxin genes of campylobacter jejuni isolates from danish turkeys by pcr and cytolethal distending toxin production of the isolates. | a total of 117 campylobacter jejuni isolates from danish turkeys were tested for the presence of seven virulence and toxin genes by pcr. one hundred seventeen (100%) isolates were positive for flaa, cadf, and ceue gene primers. one hundred three (88%) isolates were positive for cdt gene cluster pcr detection (cdt gene cluster-pcr), whereas 101 (86.3%), 102 (87.2%), and 110 (94%) isolates were positive for cdta-, cdtb-, and cdtc-pcr, respectively. only 39 (33.3%) isolates were positive for virb11 ... | 2004 | 15508626 |
| antimicrobial resistance in campylobacter strains isolated from french broilers before and after antimicrobial growth promoter bans. | the antimicrobial susceptibility of campylobacter strains isolated from standard and free-range broilers in 1992-1996 and 2001-2002 was studied. | 2004 | 15537699 |
| survival of campylobacter jejuni on various fresh produce. | the survival of seven human and two chicken campylobacter jejuni strains, with known penner heat-stable (hs) serotypes and pulsed-field gel electrophoresis (pfge) genotypes, was investigated on fresh-cut iceberg lettuce. in addition, the survival of four selected c. jejuni strains was assessed on cantaloupe pieces, cucumber slices, grated carrot and strawberries. fresh produce was inoculated with 10(5) to 10(7) colony-forming units (cfu) of c. jejuni per gram, and the bacterium was enumerated us ... | 2004 | 15541805 |
| survival of cold-stressed campylobacter jejuni on ground chicken and chicken skin during frozen storage. | campylobacter jejuni is prevalent in poultry, but the effect of combined refrigerated and frozen storage on its survival, conditions relevant to poultry processing and storage, has not been evaluated. therefore, the effects of refrigeration at 4 degrees c, freezing at -20 degrees c, and a combination of refrigeration and freezing on the survival of c. jejuni in ground chicken and on chicken skin were examined. samples were enumerated using tryptic soy agar containing sheep's blood and modified c ... | 2004 | 15574906 |
| detection of campylobacter and shigella species in food samples using an array biosensor. | campylobacter and shigella bacteria are common causes of food- and water-borne illness worldwide. there is a current need in food, medical, environmental, and military markets for a rapid and user-friendly method of detecting such pathogens. the array biosensor developed at the nrl encompasses these qualities. in this study, 25-min, sandwich immunoassays were developed for the detection of campylobacter and shigella species in both buffer and a variety of food and beverage samples. the limit of ... | 2004 | 14719894 |
| antagonistic activities of several bacteria on in vitro growth of 10 strains of campylobacter jejuni/coli. | chicken meat contaminated with campylobacter jejuni can be the source of human enteritis. to decrease the risk of human infection, campylobacter should be controlled at farm levels. orally given probiotic bacteria could prevent colonisation of chicken with pathogenic bacteria like campylobacter. the aim of this study was to investigate the effect of different bacteria on campylobacter growth. our results demonstrated that bacteria isolated from conventional chicken had potential inhibitory activ ... | 2004 | 14672829 |
| chicken juice, a food-based model system suitable to study survival of campylobacter jejuni. | the purpose of this study was to develop a food-based model system that resembles the environment that campylobacter jejuni experiences on raw poultry products and use this model system to investigate growth and survival of the bacterium. | 2004 | 14687218 |
| l-serine catabolism via an oxygen-labile l-serine dehydratase is essential for colonization of the avian gut by campylobacter jejuni. | campylobacter jejuni is a microaerophilic, asaccharolytic bacterium. the identity of the carbon and energy sources used by c. jejuni in vivo is unknown, but the genome sequence of strain nctc11168 indicates the presence of genes for catabolism of a limited range of amino acids, including serine. specific omission of l-serine from a defined medium containing a mixture of amino acids led to a dramatic decrease in cell yields. as c. jejuni does not have a biosynthetic serine requirement, this suppo ... | 2004 | 14688104 |
| the genome-sequenced variant of campylobacter jejuni nctc 11168 and the original clonal clinical isolate differ markedly in colonization, gene expression, and virulence-associated phenotypes. | the genome sequence of the enteric bacterial pathogen campylobacter jejuni nctc 11168 (11168-gs) was published in 2000, providing a valuable resource for the identification of c. jejuni-specific colonization and virulence factors. surprisingly, the 11168-gs clone was subsequently found to colonize 1-day-old chicks following oral challenge very poorly compared to other strains. in contrast, we have found that the original clinical isolate from which 11168-gs was derived, 11168-o, is an excellent ... | 2004 | 14702320 |
| detection of salmonella enterica serovar typhimurium by using a rapid, array-based immunosensor. | the multianalyte array biosensor (maab) is a rapid analysis instrument capable of detecting multiple analytes simultaneously. rapid (15-min), single-analyte sandwich immunoassays were developed for the detection of salmonella enterica serovar typhimurium, with a detection limit of 8 x 10(4) cfu/ml; the limit of detection was improved 10-fold by lengthening the assay protocol to 1 h. s. enterica serovar typhimurium was also detected in the following spiked foodstuffs, with minimal sample preparat ... | 2004 | 14711637 |
| development of predictive models for the survival of campylobacter jejuni (atcc 43051) on cooked chicken breast patties and in broth as a function of temperature. | the objective of this study was to model the kinetics of the survival of campylobacter jejuni on cooked chicken breast patties and in broth as a function of temperature. both patties and broth were inoculated with 10(6) stationary-phase cells of a single strain of c. jejuni (atcc 43051) and incubated at constant temperatures from 4 to 30 degrees c in 2 degrees c increments under aerobic conditions. in most cases, a three-phase linear model fit the primary survival curves well (r2 = 0.97 to 0.99) ... | 2004 | 14717353 |
| [incidence of the virulence markers iam in campylobacter jejuni and campylobacter coli strains isolated from poultry carcases]. | previously found dna sequence (iam), correlated with the invasiveness of campylobacter strains, became a starting point for present investigation. main goal of this study was to isolate number of campylobacter strains from chicken carcasses, to determine their taxonomic position and to establish the presence of iam sequence in their genoms. it was found, that invasion associated marker is present in all campylobacter coli strains and in majority but not all campylobacter jejuni. this may confirm ... | 2004 | 19097580 |
| enhanced in vivo fitness of fluoroquinolone-resistant campylobacter jejuni in the absence of antibiotic selection pressure. | campylobacter jejuni, a major foodborne human pathogen, has become increasingly resistant to fluoroquinolone (fq) antimicrobials. by using clonally related isolates and genetically defined mutants, we determined the fitness of fq-resistant campylobacter in chicken (a natural host and a major reservoir for c. jejuni) in the absence of antibiotic selection pressure. when monoinoculated into the host, fq-resistant and fq-susceptible campylobacter displayed similar levels of colonization and persist ... | 2005 | 15634738 |
| sequence typing and comparison of population biology of campylobacter coli and campylobacter jejuni. | a multilocus sequence typing (mlst) scheme that uses the same loci as a previously described system for campylobacter jejuni was developed for campylobacter coli. the c. coli-specific primers were validated with 53 isolates from humans, chickens, and pigs, together with 15 penner serotype reference isolates. the nucleotide sequence of the flaa short variable region (svr) was determined for each isolate. these sequence data were compared to equivalent information for 17 c. jejuni isolates represe ... | 2005 | 15634992 |
| rapid detection and differentiation of pathogenic campylobacter jejuni and campylobacter coli by real-time pcr. | a two-tube real-time assay, developed in a lightcycler, was used to detect, identify and differentiate campylobacter jejuni and campylobacter coli from all other pathogenic members of the family campylobacteriaceae. in the first assay, continuous monitoring of the fluorescence resonance energy transfer (fret) signal acquired from the hybridisation of two adjacent fluoroprobes, a specific fitc probe 5'-gtgctagcttgctagaacttagaga-fitc-3') and a universal downstream probe cy5 (5'-cy5-aggtgitgcatggit ... | 2005 | 15636755 |
| prevalence and subtypes of ciprofloxacin-resistant campylobacter spp. in commercial poultry flocks before, during, and after treatment with fluoroquinolones. | five commercial broiler chicken flocks were treated with either difloxacin or enrofloxacin for a clinically relevant infection, as instructed by a veterinarian. campylobacters were isolated from individual fecal samples and from samples associated with the broiler environment before, during, and after treatment. ciprofloxacin-resistant campylobacter jejuni and/or c. coli strains were detected pretreatment in four flocks, but they constituted a very small proportion of the campylobacters present. ... | 2005 | 15673753 |
| incidence and mechanism of ciprofloxacin resistance in campylobacter spp. isolated from commercial poultry flocks in the united kingdom before, during, and after fluoroquinolone treatment. | five commercial broiler flocks were treated with a fluoroquinolone for a clinically relevant infection. fresh feces from individual chickens and environmental samples were cultured for campylobacters before, during, and weekly posttreatment until slaughter. both campylobacter jejuni and c. coli were isolated during all treatment phases. an increased proportion of quinolone-resistant strains was seen during treatment, and these strains persisted posttreatment. one quinolone-resistant isolate of e ... | 2005 | 15673754 |
| genotype dynamics of campylobacter jejuni in a broiler flock. | we investigated the genotype diversity and dynamics of campylobacter in a commercial broiler flock during rearing and slaughter. in total, 220 campylobacter jejuni isolates collected on four sampling occasions during rearing and from routine sampling during slaughter were subtyped by smai macrorestriction and pulsed-field gel electrophoresis, pfge. eight different smai types were found. during rearing, a subsequent addition of genotypes occurred, with two smai types found at 2 weeks of age and s ... | 2005 | 15737480 |
| tetracycline resistance of australian campylobacter jejuni and campylobacter coli isolates. | tetracycline resistance in campylobacter is encoded by the tet(o) gene and is usually associated with conjugative plasmids. little was known about tetracycline resistance in australian campylobacter species, therefore we investigated this resistance in 41 campylobacter jejuni and five campylobacter coli strains from humans and healthy chickens. | 2005 | 15743900 |
| enumeration and diversity of campylobacters and bacteriophages isolated during the rearing cycles of free-range and organic chickens. | campylobacters and campylobacter-specific bacteriophages were isolated and enumerated during the rearing cycle of free-range (56 days) and organic chickens (73 days) at 3-day intervals from hatching until slaughter. in both flocks campylobacter jejuni was the initial colonizer but campylobacter coli was detected more frequently from 5 weeks of age. the diversity of the campylobacter isolates was examined by pulsed-field gel electrophoresis of smai-digested genomic dna and antimicrobial resistanc ... | 2005 | 15746327 |
| risk factors for domestic sporadic campylobacteriosis among young children in sweden. | a case-control study was conducted in sweden to study risk factors for domestically acquired campylobacter jejuni/coli infections among children aged less than 6 y. a total of 126 cases, reported to the national surveillance system were recruited over 1 y. controls, selected from the population register, were matched to the cases by age, gender, place of residence and time of infection of the case. information was gathered by posted questionnaires. two separate conditional regression models were ... | 2005 | 15764201 |
| prevalence of campylobacter, salmonella, and escherichia coli on the external packaging of raw meat. | during september and october 2002, 3,662 prepackaged raw meat samples were collected to evaluate the extent and nature of microbiological contamination on external surfaces of the packaging, which could potentially cross-contaminate ready-to-eat foods during and after purchase. salmonella was detected on two (<1%) samples of external packaging (both from raw chicken), and campylobacter was detected on 41 (1.1%) samples of external packaging. the external packaging of game fowl exhibited the high ... | 2005 | 15771168 |
| comparison of methods for the isolation of thermotolerant campylobacter from poultry. | human campylobacteriosis has become the major cause of foodborne gastroenteritis in industrialized countries. although there have been numerous studies investigating the prevalence of campylobacter in animals and raw meat, sensitive and low-cost detection methods are needed to implement effective control measures during primary production and to use as tools in risk assessment studies. thermophilic campylobacter spp. in naturally contaminated (n = 64) and inoculated (n = 16) broiler samples were ... | 2005 | 15771193 |
| pcr identification of campylobacter coli and campylobacter jejuni by partial sequencing of virulence genes. | the objective of this study was to utilize a multiplex pcr assay for concurrent detection of campylobacter spp. and c. coli or c. jejuni, using probes derived from genes cadf and ceue and an undefined virulence gene. a total of 97 campylobacter strains, isolated from turkey litter (n=74), chicken livers (n=15) and clinical (n=8) samples, were speciated using the pcr-based assay. pcr amplification of the isolates identified a 400-bp cadf gene, conserved in campylobacter species, an 894-bp ceue ge ... | 2005 | 15797819 |
| identification and molecular epidemiology of campylobacter coli isolates from human gastroenteritis, food, and animal sources by amplified fragment length polymorphism analysis and penner serotyping. | campylobacter coli is an infrequently studied but important food-borne pathogen with a wide natural distribution. we investigated its molecular epidemiology by use of amplified fragment length polymorphism (aflp)-based genotyping and penner serotyping. serotype reference strains and 177 danish isolates of diverse origin identified by routine phenotyping as c. coli were examined. molecular tools identified some 12% of field isolates as campylobacter jejuni, emphasizing the need for improved ident ... | 2005 | 15812025 |
| presence of campylobacter jejuni in various organs one hour, one day, and one week following oral or intracloacal inoculations of broiler chicks. | day-old broiler chicks (n=30) were obtained from a commercial hatchery and inoculated, either orally or intracloacally, with a characterized strain of campylobacter jejuni. at 1 hr, 1 day, and 1 wk after inoculation, broilers (n = 5) from the orally and intracloacally inoculated groups along with control birds (n=4) were humanely killed by cervical dislocation. the broilers from the control and treatment groups were aseptically opened, and the thymus, spleen, liver/gallbladder, bursa of fabriciu ... | 2005 | 15839431 |
| cytolethal distending toxin (cdt)-negative campylobacter jejuni strains and anti-cdt neutralizing antibodies are induced during human infection but not during colonization in chickens. | the cytolethal distending toxin (cdt) of campylobacter jejuni was detectable, using an in vitro assay, in most but not all of 24 strains tested. the reason for the absence of toxin activity in these naturally occurring cdt-negative c. jejuni strains was then investigated at the genetic level. cdt is encoded by three highly conserved genes, cdta, -b, and -c. in the cdt-negative strains, two types of mutation were identified. the cdt activities of c. jejuni strains possessing both types of mutatio ... | 2005 | 15845513 |
| an outbreak of campylobacter jejuni enteritis in a school of madrid, spain. | an outbreak of gastroenteritis caused by campylobacter infection was identified in may 2003 in a school in madrid, spain. eighty one cases were identified in a total of 253 people studied. a retrospective cohort study showed that a custard made with ultra high temperature (uht) milk was associated with illness (rr: 3.15; 95% ci: 1.25-7.93). the custard was probably contaminated with campylobacter jejuni from a raw chicken prepared a day previously in the same kitchen. our recommendations were to ... | 2005 | 15879642 |
| bacteriophage control of foodborne bacteriat. | bacteriophages are measurable components of the natural microflora in the food production continuum from the farm to the retail outlet. phages are remarkably stable in these environments and are readily recovered from soil, sewage, water, farm and processing plant effluents, feces, and retail foods. purified high-titer phage lysates have been used for the species-specific control of bacteria during the pre- and postharvest phases of food production and storage. for example, the inhibition of the ... | 2005 | 15895751 |
| host genes affect intestinal colonisation of newly hatched chickens by campylobacter jejuni. | campylobacter jejuni is the leading cause of food-borne gastro-enteritis and infection can be followed by severe clinical complications, such as the autoimmune neuropathy guillain-barré syndrome. poultry meat is considered to be a common source of infection, with most flocks infected from 2 to 3 weeks of age. we have examined the effect of host genetics on the colonisation levels of c. jejuni in chickens. chicks from different inbred lines were challenged with 10(7) to 10(8) cfu of c. jejuni 14n ... | 2005 | 15900496 |
| the campylobacter jejuni response regulator, cbrr, modulates sodium deoxycholate resistance and chicken colonization. | two-component regulatory systems play a major role in the physiological response of bacteria to environmental stimuli. such systems are composed of a sensor histidine kinase and a response regulator whose ultimate function is to affect the expression of target genes. response regulator mutants of campylobacter jejuni strain f38011 were screened for sensitivity to sodium deoxycholate. a mutation in cj0643, which encodes a response regulator with no obvious cognate histidine kinase, resulted in an ... | 2005 | 15901688 |
| mama-pcr assay for the detection of point mutations associated with high-level erythromycin resistance in campylobacter jejuni and campylobacter coli strains. | twenty campylobacter jejuni and 16 campylobacter coli strains isolated from humans and food/animals, including 17 isolates resistant to erythromycin, were analyzed. a combined mismatch amplification mutation assay-pcr technique was developed to detect the mutations a 2074 c and a 2075 g in the 23s rrna gene associated with erythromycin resistance. all high-level erythromycin-resistant strains examined by dna sequencing carried the transition mutation a 2075 g, whereas no isolate carried the a 20 ... | 2005 | 15927294 |
| prevalence of potential virulence markers in polish campylobacter jejuni and campylobacter coli isolates obtained from hospitalized children and from chicken carcasses. | the pathogenicity of thermotolerant campylobacter species, common food-borne pathogens, depends on certain factors unevenly distributed among strains of different origin. the prevalence of such markers has never been examined in a population of polish campylobacter strains of human and poultry origin. therefore, we analysed the presence of the cadf, cdta, cdtb and cdtc genes and the iam sequence in campylobacter jejuni (n = 115) and campylobacter coli (n = 57) isolates from children with diarrho ... | 2005 | 15947425 |
| pet dogs and chicken meat as reservoirs of campylobacter spp. in barbados. | campylobacter spp. are the second most common pathogen isolated from stools of patients with gastroenteritis in barbados. the aim of this study was to identify reservoirs of campylobacter and the likely source(s) of human infection. fecal specimens from 596 animals and 311 samples of animal food products were analyzed for the presence of campylobacter spp. by standard culture techniques. isolates were characterized by conventional phenotypic tests, confirmed by latex agglutination and pcr with g ... | 2005 | 15956378 |
| occurrence of campylobacter spp. in cecal contents among commercial broilers in iceland. | campylobacter jejuni remains the most frequently reported bacterial cause of human gastroenteritis in nordic countries. the primary source of transmission to humans is suggested as mishandled raw poultry or consuming improperly prepared chicken. the focus of this report was to characterize the prevalence and cell numbers of the organism within the commercial icelandic poultry industry. commercial broiler flocks were sampled from may 2001 through 2003 in a total population study. at the slaughter ... | 2005 | 15992302 |
| detection of campylobacter jejuni in naturally contaminated chicken skin by melting peak analysis of amplicons in real-time pcr. | contamination of poultry by campylobacter spp. is a significant source of human diarrheal diseases. traditional methods currently used to detect campylobacter in foods are time-consuming and labor-intensive. in this study, primers designed for the campylobacter jejuni cadf gene sequence were used in a sybr green i real-time pcr assay as an alternative to a conventional bacteriological method for the rapid detection of c. jejuni from poultry. twelve portions of chicken purchased from two local gr ... | 2005 | 15996780 |
| paenibacillus polymyxa purified bacteriocin to control campylobacter jejuni in chickens. | campylobacter spp. cause numerous foodborne diseases. poultry is thought to be a significant source of this zoonosis. although many interventions designed to control this agent have been researched, none have succeeded. we evaluated a bacteriocin-based treatment to reduce campylobacter jejuni colonization in poultry. a previously described purified bacteriocin (class iia; molecular mass, 3,864 da), secreted by paenibacillus polymyxa nrrl-b-30509, was microencapsulated in polyvinylpyrrolidone, an ... | 2005 | 16013385 |
| phage therapy reduces campylobacter jejuni colonization in broilers. | the effect of phage therapy in the control of campylobacter jejuni colonization in young broilers, either as a preventive or a therapeutic measure, was tested. a prevention group was infected with c. jejuni at day 4 of a 10-day phage treatment. a therapeutic group was phage treated for 6 days, starting 5 days after c. jejuni colonization of the broilers had been established. treatment was monitored by enumerating campylobacter colony forming units (cfu) and phage plaque forming units (pfu) from ... | 2005 | 16024187 |
| genotyping of campylobacter jejuni strains from danish broiler chickens by restriction fragment length polymorphism of the lps gene cluster. | to apply and evaluate lg (lps genes) genotyping, which is a genotyping method based on a cluster of genes involved in the synthesis of surface lipopolysaccharides (lps) in campylobacter species, for typing of campylobacter jejuni isolates obtained from danish broiler chickens. furthermore, the lg genotyping method was used to study the genetic stability of four c. jejuni strains after gastrointestinal passage through experimentally infected chickens. | 2005 | 16033471 |
| development and application of oligonucleotide probes for in situ detection of thermotolerant campylobacter in chicken faecal and liver samples. | based on campylobacter 16s- and 23s-rrna sequence data oligonucleotide probes specific for thermotolerant campylobacters and for members of the genus campylobacter have been developed. the 16s-rrna-targeted probe camp 653, recommended for a comprehensive detection of members of the genus campylobacter, specifically detected all campylobacter strains used in this study. detection of thermotolerant species has been achieved by the 23s-rrna-targeted probe cajeco1427. optimal hybridisation condition ... | 2005 | 16061298 |
| detection of pathogenic bacteria in food samples using highly-dispersed carbon particles. | there is an unmet need for detection methods that can rapidly and sensitively detect food borne pathogens. a flow through immunoassay system utilizing highly dispersed carbon particles and an amperometric technique has been developed and optimized. a sandwich immunoassay format was utilized in which pathogenic cells were captured by antibodies immobilized onto activated carbon particles, and labeled with horseradish peroxidase (hrp) conjugated antibodies. flow of the peroxidase substrates result ... | 2005 | 16076439 |
| genetic diversity of campylobacter sp. isolates from retail chicken products and humans with gastroenteritis in central michigan. | multilocus sequencing was used to compare campylobacter sp. strains isolated from retail chicken products and humans with gastroenteritis in central michigan. sequence comparisons demonstrated overlapping diversity between chicken and human isolates. campylobacter jejuni isolates from clinical sources had a greater diversity of flagellin alleles and a higher rate of quinolone resistance than isolates from retail chicken products. | 2005 | 16081984 |
| inhibition of clostridium perfringens by a novel strain of bacillus subtilis isolated from the gastrointestinal tracts of healthy chickens. | the objectives of this study were to isolate beneficial strains of microorganisms from the gastrointestinal tracts of healthy chickens and to screen them against clostridium perfringens, a causative agent of necrotic enteritis in poultry. one of the bacteria isolated, a strain of bacillus subtilis, was found to possess an anticlostridial factor that could inhibit the c. perfringens atcc 13124 used in this study. the anticlostridial factor produced by b. subtilis pb6 was found to be fully or part ... | 2005 | 16085801 |
| correlation of campylobacter bacteriophage with reduced presence of hosts in broiler chicken ceca. | campylobacter jejuni and campylobacter-specific bacteriophage were enumerated from broiler chicken ceca selected from 90 united kingdom flocks (n = 205). c. jejuni counts in the presence of bacteriophage (mean log(10) 5.1 cfu/g) were associated with a significant (p < 0.001) reduction compared to samples with campylobacter alone (mean log(10) 6.9 cfu/g). | 2005 | 16085889 |
| genetic profiling of campylobacter jejuni strains from humans infected in sweden or in thailand, and from healthy swedish chickens, studied by pulsed-field gel electrophoresis (pfge). | the major objective of the study was to explore the genomic diversity between campylobacter jejuni (c.jejuni) from different sources as a tool for epidemiological considerations. subtyping was performed using pulsed-field gel electrophoresis (pfge) and the enzyme used for cleavage was smai. isolates originated from humans infected in sweden (n=49) and thailand (n=32) and from healthy swedish chickens (n=51). eight pfge groups were formed in a dendrogram and 48% of the isolates belonged to 1 of t ... | 2005 | 16138427 |
| reservoir competence of lesser mealworm (coleoptera: tenebrionidae) for campylobacter jejuni (campylobacterales: campylobacteraceae). | the lesser mealworm, alphitobius diaperinus (panzer), is a carrier of campylobacter spp. in poultry facilities; however, the beetle's importance in the epidemiology of campylobacteriosis is not known. a series of laboratory experiments were designed to test the vector and reservoir competence of the lesser mealworm for campylobacter jejuni. in the first experiment, c. jejuni was swabbed onto the outer surface of adult and larval beetles to determine how long bacteria can survive on the beetles' ... | 2005 | 15691007 |
| factors affecting the pressure resistance of some campylobacter species. | to compare pressure resistance between strains of campylobacter jejuni, campylobacter coli, campylobacter lari and campylobacter fetus, and to investigate the effect of suspending medium on pressure resistance of sensitive and more resistant strains. | 2005 | 16162138 |
| effects of orally administered tetracycline on the intestinal community structure of chickens and on tet determinant carriage by commensal bacteria and campylobacter jejuni. | there is a growing concern that antibiotic usage in animal production has selected for resistant food-borne bacteria. since tetracyclines are common therapeutic antibiotics used in poultry production, we sought to evaluate the effects of oral administration on the resistance of poultry commensal bacteria and the intestinal bacterial community structure. the diversity indices calculated from terminal restriction fragment length polymorphism (t-rflp) analysis of 16s rrna amplicons did not indicate ... | 2005 | 16204498 |
| genomic diversity of campylobacter coli and campylobacter jejuni isolates recovered from free-range broiler farms and comparison with isolates of various origins. | in many industrialized countries, the incidence of campylobacteriosis exceeds that of salmonellosis. campylobacter bacteria are transmitted to humans mainly in food, especially poultry meat products. total prevention of campylobacter colonization in broiler flocks is the best way to reduce (or eliminate) the contamination of poultry products. the aim of this study was to establish the sources and routes of contamination of broilers at the farm level. molecular typing methods (dna macrorestrictio ... | 2005 | 16204541 |
| comparative phylogenomics of the food-borne pathogen campylobacter jejuni reveals genetic markers predictive of infection source. | campylobacter jejuni is the predominant cause of bacterial gastroenteritis worldwide, but traditional typing methods are unable to discriminate strains from different sources that cause disease in humans. we report the use of genomotyping (whole-genome comparisons of microbes using dna microarrays) combined with bayesian-based algorithms to model the phylogeny of this major food-borne pathogen. in this study 111 c. jejuni strains were examined by genomotyping isolates from humans with a spectrum ... | 2005 | 16230626 |
| campylobacter susceptibility to ciprofloxacin and corresponding fluoroquinolone concentrations within the gastrointestinal tracts of chickens. | this study evaluated the relationship between campylobacter susceptibility and enteric fluoroquinolone concentrations in chickens treated with different doses of enrofloxacin. | 2005 | 16238734 |
| a robotic dna purification protocol and real-time pcr for the detection of campylobacter jejuni in foods. | primers designed to amplify a campylobacter jejuni cadf gene sequence were used in an sybr green i real-time pcr assay as an alternative to conventional bacteriological methods for the rapid detection of c. jejuni in foods. twenty-five grams of chicken skin (breast and thigh) was contaminated by adding approximately 1, 10, or 50 cfu of c. jejuni atcc 35560. twenty-five grams of pork and 25-ml aliquots of milk were also inoculated with 1 and 10 cfu of the pathogen. the samples were incubated in b ... | 2005 | 16245718 |
| reduction in flock prevalence of campylobacter spp. in broilers in norway after implementation of an action plan. | an action plan against thermophilic campylobacter spp. in norwegian broilers was implemented in may 2001. the action plan consists of three parts: a surveillance program including all norwegian broiler flocks slaughtered before 50 days of age, a follow-up advisory service on farms delivering flocks positive for campylobacter spp., and surveys of broiler meat products at the retail level. this article presents results covering the inclusive 3-year period between 2002 and 2004. during this period, ... | 2005 | 16245734 |
| bacteriophage therapy to reduce campylobacter jejuni colonization of broiler chickens. | colonization of broiler chickens by the enteric pathogen campylobacter jejuni is widespread and difficult to prevent. bacteriophage therapy is one possible means by which this colonization could be controlled, thus limiting the entry of campylobacters into the human food chain. prior to evaluating the efficacy of phage therapy, experimental models of campylobacter colonization of broiler chickens were established by using low-passage c. jejuni isolates hpc5 and giic8 from united kingdom broiler ... | 2005 | 16269681 |
| efficacy of electrolyzed water in the prevention and removal of fecal material attachment and its microbicidal effectiveness during simulated industrial poultry processing. | this study was undertaken to investigate the efficacy of alkaline and acidic electrolyzed (eo) water in preventing and removing fecal contaminants and killing campylobacter jejuni on poultry carcasses under simulated industrial processing conditions. new york dressed and defeathered chicken carcasses spot-inoculated with cecal material or c. jejuni were subjected to spraying treatment with alkaline eo or 10% trisodium phosphate (tsp) water or combinations of spraying and immersion treatments wit ... | 2005 | 16463977 |
| signature-tagged transposon mutagenesis studies demonstrate the dynamic nature of cecal colonization of 2-week-old chickens by campylobacter jejuni. | we have constructed plasmids to be used for in vitro signature-tagged mutagenesis (stm) of campylobacter jejuni and used these to generate stm libraries in three different strains. statistical analysis of the transposon insertion sites in the c. jejuni nctc 11168 chromosome and the plasmids of strain 81-176 indicated that their distribution was not uniform. visual inspection of the distribution suggested that deviation from uniformity was not due to preferential integration of the transposon int ... | 2005 | 16332783 |
| campylobacter in food animals and humans in northern thailand. | cross-sectional, longitudinal, and case-control studies were conducted to describe the epidemiology of campylobacter in chickens, swine, dairy cows, farm workers, nonfarm residents, and children with diarrhea. samples were collected in chiang mai and lamphung provinces of northern thailand from 2000 through 2003. a total of 2,360 samples were processed. results from the cross-sectional study indicated that the prevalences of campylobacter in chickens at the farm, slaughterhouse, and market were ... | 2005 | 16355821 |
| emergence of fluoroquinolone resistance in campylobacter jejuni in chickens exposed to enrofloxacin treatment at the inherent dosage licensed in japan. | the objective of the present study was to determine whether selection of fluoroquinolone resistance could be easily induced in campylobacter jejuni-colonized chickens by treatment with enrofloxacin of representative fluoroquinolones at the inherent dosage licensed in japan (50 ppm in drinking water for 3 days). in the case of isolates from chickens of study 1, an increase in the population of susceptible isolates appeared after the cessation of treatment and maintained throughout the experiments ... | 2005 | 16364022 |
| identification of host-associated alleles by multilocus sequence typing of campylobacter coli strains from food animals. | campylobacter coli is a food-borne pathogen associated increasingly with human gastroenteritis. c. coli has a high prevalence in swine, but is isolated also from cattle and poultry. multilocus sequence typing (mlst) systems have been developed to differentiate c. coli strains. although substantial allelic diversity was identified across all seven c. coli mlst loci, no correlations were made in two previous studies between allele or sequence type (st) and the source of the organism. however, this ... | 2006 | 16385134 |
| stable concentrated emulsions of the 1-monoglyceride of capric acid (monocaprin) with microbicidal activities against the food-borne bacteria campylobacter jejuni, salmonella spp., and escherichia coli. | of 11 fatty acids and monoglycerides tested against campylobacter jejuni, the 1-monoglyceride of capric acid (monocaprin) was the most active in killing the bacterium. various monocaprin-in-water emulsions were prepared which were stable after storage at room temperature for many months and which retained their microbicidal activity. a procedure was developed to manufacture up to 500 ml of 200 mm preconcentrated emulsions of monocaprin in tap water. the concentrates were clear and remained stabl ... | 2006 | 16391087 |
| genotyping of campylobacter spp. from retail meats by pulsed-field gel electrophoresis and ribotyping. | to determine the genetic relatedness of campylobacter spp. from retail meat products, and compare the discriminatory power of pulsed-field gel electrophoresis (pfge) and automatic ribotyping. | 2006 | 16405698 |
| antimicrobial resistance among campylobacter jejuni isolated from raw poultry meat at retail level in denmark. | campylobacter jejuni isolated from raw poultry meat collected at retail shops in denmark in the period 1996-2003 were tested for susceptibility to seven antimicrobial agents. the food samples consisted of raw chicken meat and other raw poultry meat of domestic or imported origin. the highest levels of resistance among c. jejuni were observed for tetracycline, nalidixic acid and ciprofloxacin, whereas macrolide resistance was rarely detected. c. jejuni originating from other poultry meat (mainly ... | 2006 | 16410028 |
| antibiotic resistance of campylobacter jejuni and campylobacter coli isolated from hog, beef, and chicken carcass samples from provincially inspected abattoirs in ontario. | campylobacterjejuni is one of the most common causes of bacterial foodborne infection in the united states, and there are reports of resistance of campylobacter spp. to antimicrobial agents used for the treatment of gastroenteritis. the purpose of this study was to determine the antimicrobial resistance patterns of campylobacter spp. isolated from hog, beef, and chicken carcasses from provincially inspected abattoirs in ontario. the agar dilution method was performed to measure antimicrobial res ... | 2006 | 16416896 |
| growth temperature of four campylobacter jejuni strains influences their subsequent survival in food and water. | to determine if campylobacter jejuni grown at 37 and 42 degrees c have different abilities to survive on beef and chicken, and in water. | 2006 | 17083703 |
| characterization of macrolide-resistant campylobacter coli isolates from food-producing animals on farms across japan during 2004. | we investigated the susceptibilities against 7 antimicrobial agents in campylobacter jejuni and c. coli isolates from food-producing animals in 2004. in comparison with the results of past surveillance, no significant difference was observed in resistance rates against all of the antimicrobials tested in campylobacter isolates. however, slight increase of erythromycin (em) resistance was found in c. coli isolates from pigs. we examined the mutation of the 23s rrna gene and their susceptibilities ... | 2006 | 17085893 |
| the effect of lemon, orange and bergamot essential oils and their components on the survival of campylobacter jejuni, escherichia coli o157, listeria monocytogenes, bacillus cereus and staphylococcus aureus in vitro and in food systems. | to investigate the effectiveness of oils and vapours of lemon (citrus limon), sweet orange (citrus sinensis) and bergamot (citrus bergamia) and their components against a number of common foodborne pathogens. | 2006 | 17105553 |
| a comparative study of two food model systems to test the survival of campylobacter jejuni at -18 degrees c. | the survival of campylobacter jejuni nctc 11168 was tested at freezing conditions (-18 degrees c) over a period of 32 days in two food models that simulated either (i) the chicken skin surface (skin model) or (ii) the chicken juice in and around a broiler carcass (liquid model). in the skin model, cells were suspended in chicken juice or brain heart infusion broth (bhib) and added to 4-cm2 skin pieces, which were subsequently stored at -18 degrees c. in the liquid model, cells were suspended in ... | 2006 | 17133806 |
| study on the prevalence of campylobacter spp. from chicken meat in hanoi, vietnam. | campylobacter spp. is considered to be the most common bacterial cause of human gastroenteritis worldwide. in developing countries, campylobacter spp. diarrhea is an important cause of childhood morbidity. chicken meat is known to be a major source of campylobacteriosis infection in the world. the purpose of this study was to investigate the prevalence of campylobacter spp. in chicken meat. a total of 100 samples from breast part of chicken carcass were collected from retail market in hanoi. the ... | 2006 | 17135525 |
| a pilot study on post-evisceration contamination of broiler carcasses and ready-to-sell livers and intestines (mala) with campylobacter jejuni and campylobacter coli in a high-throughput south african poultry abattoir. | to assess post-evisceration contamination of broiler carcasses, 300 samples were randomly selected during routine slaughter in the winter of 2004. the samples originated from 50 chicken carcasses, taken directly after evisceration, as well as 25 samples from ready-to-sell packages of fresh intestines (mala) and livers. the samples were taken in batches over a period of 4 weeks to allow randomised sampling from different farms of origin. conventional culture-based detection methods of campylobact ... | 2006 | 17137050 |
| survey of chicken abattoir for the presence of campylobacter jejuni and campylobacter coli. | the genus campylobacter is of great importance to public health because it includes several species that may cause diarrhea. these species may be found in water, food and in the intestinal tract of chickens. this study investigated the presence of campylobacter jejuni and campylobacter coli in chicken abattoirs in são paulo state, brazil. a total of 288 samples of feces, feathers, scald water, evisceration water, chiller water, and the rinse water of eviscerated, not eviscerated and chilled carc ... | 2006 | 17221125 |
| effect of cytolethal distending toxin of campylobacter jejuni on adhesion and internalization in cultured cells and in colonization of the chicken gut. | campylobacter jejuni produces cytolethal distending toxin (cdt) that causes host cells to arrest during their cell cycle and that is involved in the pathogenesis of inflammatory diarrhea in humans. to assess the role of cdt in adherence and invasion of different cultured host cells (hela and hd-11) and in colonization of the chicken intestine, the genes of c jejuni nctc11168 encoding the toxin subunits (cdta, cdtb, and cdtc) were inactivated by insertional mutagenesis. no significant difference ... | 2006 | 17274298 |
| diversity of flaa genotypes among campylobacter jejuni isolated from six niche-market poultry species at farm and processing. | pcr-restriction fragment length polymorphism of the flagellin (flaa) gene in campylobacter jejuni was used to determine the relationships of isolates collected at the farm and throughout processing for six niche-market poultry species. this study focused on two specialty chicken products, poussin and free range, and four other specialty products, squab, duck, guinea fowl, and quail. cloacal and carcass samples were collected from three flocks from each of the six niche species. three processing ... | 2006 | 16496569 |
| colonizing capability of campylobacter jejuni genotypes from low-prevalence avian species in broiler chickens. | genetic variations in campylobacter jejuni or host factors result in low prevalence rates among nonchicken poultry species. the objective of this study was to determine the colonizing potential, in broiler chickens, of c. jejuni that was recovered from low-prevalence avian species. twenty-day-old campylobacter-negative broiler chicks were inoculated by oral gavage with genetically different primary isolates of c. jejuni recovered from squab, duck, or chicken. serial sampling and microbiologic te ... | 2006 | 16496585 |
| human and chicken antibodies to gangliosides following infection by campylobacter jejuni. | campylobacteriosis is frequently associated with guillain-barré syndrome. poultry are frequently highly colonized with campylobacter jejuni and are a major foodborne vehicle for campylobacteriosis. in this study, high titer anti-gm1 antibodies were found in the serum of a laboratory worker who developed campylobacteriosis. the microbiologically confirmed strain vla2/18 (non-serotyped) was isolated from the worker and subsequently inoculated into chickens, resulting in high titers of serum antibo ... | 2006 | 16500643 |
| biosynthesis of the n-linked glycan in campylobacter jejuni and addition onto protein through block transfer. | in eukaryotes, n-linked protein glycosylation is a universal modification involving addition of preformed oligosaccharides to select asn-xaa-ser/thr motifs and influencing multiple biological events. we recently demonstrated that campylobacter jejuni is the first member of the bacteria to possess an n-linked glycan pathway. in this study, high-resolution magic angle spinning nuclear magnetic resonance (hr-mas nmr) was applied to probe and quantitate c. jejuni n-glycan biosynthesis in vivo. to co ... | 2006 | 16547029 |
| enumeration and identity of campylobacter spp. in italian broilers. | transmission of campylobacter to humans has been prominently associated with mishandling or improperly preparing contaminated poultry carcasses. the number of organisms per carcass represents an important measure of human exposure to the agent. therefore, we wished to estimate this public exposure over 1 yr among italian broiler carcasses. we sampled 213 broiler carcasses from rinse water samples collected from a single slaughterhouse. groups of carcasses had mean processed weights ranging from ... | 2006 | 16553289 |
| two distinct mutations in gyra lead to ciprofloxacin and nalidixic acid resistance in campylobacter coli and campylobacter jejuni isolated from chickens and beef cattle. | the aim of this study was to identify point mutations in the gyra quinolone resistance determining region (qrdr) of campylobacter coli (n = 27) and campylobacter jejuni (n = 26) that confer nalidixic acid (nal) resistance without conferring resistance to ciprofloxacin (cip). | 2006 | 16553723 |
| effects of a campylobacter jejuni infection on the development of the intestinal microflora of broiler chickens. | the effect of a campylobacter jejuni colonization on the development of the microflora of the cecum and the ileum of broiler chickens was studied using molecular methods. the infection did affect the development and complexity of the microbial communities of the ceca, but we found no permanent effect of a c. jejuni infection on the ileal microflora of the broilers. in addition, denaturant gradient gel electrophoresis (dgge) profiles generated from cecal and ileal contents revealed several dgge b ... | 2006 | 16615340 |
| campylobacter jejuni strains of human and chicken origin are invasive in chickens after oral challenge. | the aim of the study was to evaluate the colonizing ability and the invasive capacity of selected campylobacter jejuni strains of importance for the epidemiology of c jejuni in danish broiler chickens. four c. jejuni strains were selected for experimental colonization studies in day-old and 14-day-old chickens hatched from specific pathogen free (spf) eggs. of the four c. jejuni strains tested, three were penner heat-stable serotype 2, flaa type 1/1, the most common type found among broilers and ... | 2006 | 16617974 |
| campylobacter jejuni infection in broiler chickens. | day-old, straight-run broiler chickens were procured from a hatchery located in the pacific northwest. the chickens were subdivided individually into nine groups of 20 chickens. the chickens were tagged, housed in isolation chambers on wire, fed commercial broiler feed, and given water ad libitum. three isolates of campylobacter jejuni of poultry origin and one of human origin were tested in this study. various c. jejuni cultures were inoculated into 9-day-old chickens by crop gavage. four group ... | 2006 | 16617982 |
| quantification of campylobacter on the surface and in the muscle of chicken legs at retail. | the objective of this study was to determine the prevalence and numbers of campylobacter on the skin and in the muscle of chicken legs at retail to examine the external and internal contamination for an exposure assessment. furthermore, the study assessed seasonal influence on campylobacter contamination in chicken legs. of the 140 examined skin samples, 66% were positive, and the internal contamination of 115 sampled chicken legs was 27%. the enumeration of campylobacter on the surface of posit ... | 2006 | 16629016 |
| reduction of campylobacter jejuni on chicken wings by chemical treatments. | eight chemicals, including glycerol monolaurate, hydrogen peroxide, acetic acid, lactic acid, sodium benzoate, sodium chlorate, sodium carbonate, and sodium hydroxide, were tested individually or in combination for their ability to inactivate campylobacter jejuni at 4 degrees c in suspension. results showed that treatment for up to 20 min with 0.01% glycerol monolaurate, 0.1% sodium benzoate, 50 or 100 mm sodium chlorate, or 1% lactic acid did not substantially (< or = 0.5 log cfu/ml) reduce c. ... | 2006 | 16629017 |
| amplified fragment length polymorphism, serotyping, and quinolone resistance of campylobacter jejuni and campylobacter coli strains from chicken-related samples and humans in taiwan. | the high-resolution genotyping method of amplified fragment length polymorphism (aflp) analysis was used to study the genetic relationships between campylobacter jejuni isolates from chicken-related samples (n = 32) and humans (n = 27) as well as between campylobacter coli isolates from chicken-related samples (n = 27) and humans (n = 5). these isolates were collected between 1994 and 2003 in taiwan. all c. jejuni and c. coli isolates showed highly heterogeneous fingerprints. c. jejuni isolates ... | 2006 | 16629019 |
| prevalence of thermophilic campylobacter infections in humans, chickens and crows in morogoro, tanzania. | prevalence of thermophilic campylobacter infections in humans, chickens and crows was determined in a cross-sectional study that was carried out in urban and rural areas of morogoro region, tanzania during the period of january 2003 to december 2004. a total of 632 human stool samples, 536 cloacal swabs from local and broiler chickens and 22 intestinal contents from crows were screened for presence of thermophilic campylobacters using skirrow's protocol. representative campylobacter jejuni isola ... | 2006 | 16629722 |