Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| eosinophilic cystitis and cholangitis - systemic disease triggered by mycobacterium tuberculosis? | eosinophilic cystitis (ec) is a rare inflammatory disorder of the urinary tract characterized by infiltration of bladder with eosinophils. the cause remains unclear, immunological mechanisms have been implicated in pathogenesis. potential etiological factors include: tumors, allergy, parasitic infections, trauma. the disease may have a variable course, from a mild self-limiting, through common symptoms like: dysuria, hematuria, abdominal pain, tumor, to severe renal failure, with eosinophilic in ... | 2016 | 28478431 |
| coronins and their role in immunological phenomena. | coronins are a large family of proteins occurring in many eukaryotes. in mammals, seven coronin genes have been identified, evidencing that coronins 1 to 6 present classic coronin structure, while coronin 7 is a tandem coronin particle, without a spiral domain, although the best characterised coronin, in terms of both structure and function, is the mammalian coronin 1. it has been proven that they are related to regulation of actin dynamics, e.g. as a result of interaction with the complex of pr ... | 2016 | 28450807 |
| tuberculosis and pneumonia in hiv-infected children: an overview. | pneumonia remains the most common cause of hospitalization and the most important cause of death in young children. in high human immunodeficiency virus (hiv)-burden settings, hiv-infected children carry a high burden of lower respiratory tract infection from common respiratory viruses, bacteria and mycobacterium tuberculosis. in addition, pneumocystis jirovecii and cytomegalovirus are important opportunistic pathogens. as the vertical transmission risk of hiv decreases and access to antiretrovi ... | 2016 | 28702298 |
| novel vaccination approaches to prevent tuberculosis in children. | pediatric tuberculosis (tb) is an underappreciated problem and accounts for 10 % of all tb deaths worldwide. children are highly susceptible to infection with mycobacterium tuberculosis and interrupting tb spread would require the development of effective strategies to control tb transmission in pediatric populations. the current vaccine for tb, m. bovis bacille calmette-guérin (bcg), can afford some level of protection against tb meningitis and severe forms of disseminated tb in children; howev ... | 2016 | 28702297 |
| fermentation, isolation, purification and characterization of an antitubercular antibiotic from streptomyces luridus mtcc 4402. | tuberculosis, caused by mycobacterium tuberculosis, continues to be a serious public health problem around the world, and it urges the need for development of new antitubercular drugs. an antibiotic producing strain, streptomyces luridus (mtcc 4402) was earlier isolated from soil by our group. in this work, the phylogenic status was established by 16s rrna gene sequence analysis. the strain was found to be active against clinically resistant strains. the culture was grown in shake flasks in a me ... | 2016 | 28699723 |
| [determination of the rate of growth of mycobacterium tuberculosis and some of its applications]. | 2016 | 24542595 | |
| clinical research of treating tuberculous respiratory failure by sequential mechanical ventilation. | tuberculosis is kind of common chronic infectious disease, of which the pathogenic bacterium is mainly mycobacterium tuberculosis (mtb). one who is suffering severe tuberculosis may be ended with cardio-pulmonary function damage or cardio-pulmonary failure. this disease is of higher risks of complications or even high death rate, which is seriously challenging people's health and life. when a tuberculosis patient is accompanied with respiratory failure, which means the disease has developed into ... | 2016 | 28412688 |
| chest radiographic findings in primary pulmonary tuberculosis: observations from high school outbreaks. | to describe the radiographic findings of primary pulmonary tuberculosis (tb) in previously healthy adolescent patients. | 2016 | 21076586 |
| an uncommon cause of longitudinally extensive transverse myelitis. | considering that currently romania has the highest tuberculosis incidence in europe, the recognition of the infection is an acknowledged health issue. central nervous system tuberculosis accounts for approximately 1% of all cases of tuberculosis. longitudinally extensive transverse myelitis is a contiguous infl ammatory lesion of the spinal cord which involves three or more spinal segments. in tuberculosis, spinal cord involvement is rare and is usually rather due to radiculomyelitis or spinal t ... | 2016 | 28694861 |
| enrichment by hybridisation of long dna fragments for nanopore sequencing. | enrichment of dna by hybridisation is an important tool which enables users to gather target-focused next-generation sequence data in an economical fashion. current in-solution methods capture short fragments of around 200-300 nt, potentially missing key structural information such as recombination or translocations often found in viral or bacterial pathogens. the increasing use of long-read third-generation sequencers requires methods and protocols to be adapted for their specific requirements. ... | 2016 | 28785419 |
| [genetic recombiniation and protein expression detection of listeria-based tuberculosis vaccine candidates.] | genetic construction of tuberculosis vaccine candidates based on listeria(l.) monocytogenes,l.ivanovii,and evaluation their protein expression,in order to provide a novel method for research on tuberculosis controlling. | 2016 | 28598105 |
| tuberculous spondylitis of the craniovertebral junction. | craniovertebral junction tuberculosis is rare, accounting for 0.3 to 1% of all tuberculous spondylitis cases. mr imaging is the modality of choice to detect bone involvement, abscess formation and subligamentous spreading of the pus, to differentiate from other lesions affecting the craniovertebral junction, and to determine the efficacy of treatment. given the fact that surgical treatment of patients with craniovertebral junction tuberculosis has been associated with a high mortality rate rangi ... | 2016 | 28529850 |
| use of interferon-gamma release assays in a health care worker screening program: experience from a tertiary care centre in the united states. | interferon-gamma release assays including the quantiferon-tb gold in-tube test (qft-git [cellestis ltd, australia]) may be used in place of the tuberculin skin test (tst) in surveillance programs for mycobacterium tuberculosis infection control. however, data on performance and practicality of the qft-git in such programs for health care workers (hcws) are limited. | 2016 | 22536576 |
| mycobacterium tuberculosis manlam inhibits t-cell-receptor signaling by interference with zap-70, lck and lat phosphorylation. | immune evasion is required for mycobacterium tuberculosis to survive in the face of robust cd4(+) t cell responses. we have shown previously that m. tuberculosis cell wall glycolipids, including mannose capped lipoarabinomannan (manlam), directly inhibit polyclonal murine cd4(+) t cell activation by blocking zap-70 phosphorylation. we extended these studies to antigen-specific murine cd4(+) t cells and primary human t cells and found that manlam inhibited them as well. lck and lat phosphorylatio ... | 2016 | 22507872 |
| use of tuberculosis genotyping for postoutbreak monitoring. | review of routinely collected tuberculosis genotyping results following a known outbreak is a potential mechanism to examine the effectiveness of outbreak control measures. | 2016 | 22635193 |
| rapid diagnosis of mycobacterium tuberculosis infection in children using interferon-gamma release assays (igras). | diagnosis of tuberculosis (tb) in children by the tuberculin skin test (tst) poses a diagnostic challenge for physicians due to its low specificity and cross-reactivity with nontuberculous mycobacteria and bacille calmette-guerin (bcg). although interferon-gamma release assays (igras) have been shown as novel tst alternatives for diagnosis of latent tb infection (ltbi) in adults, their effectiveness is less clear in children. the present study examined quantiferon-tb gold (qft-g) responses and i ... | 2016 | 22584190 |
| what every physician should know about rapid diagnosis of mycobacterium tuberculosis infection in children using interferon-gamma release assays. | 2016 | 22584189 | |
| primary disseminated extrapulmonary multidrug resistant tuberculosis. | disseminated tuberculosis is a common mode of presentation of tuberculosis in patients both with and without hiv/aids in india. however, primary multidrug resistance in disseminated tuberculosis involving only the extrapulmonary sites in an immunocompetent adult is rare. here, we report a case of a 19-year-old man who had disseminated tuberculosis involving left pleura, pericardium, peritoneum and intraabdominal lymph nodes. he was initially taking who category i antituberculous drugs, but was n ... | 2016 | 22885212 |
| comparison of the 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide tube method with the conventional method and real-time polymerase chain reaction for the detection of rifampicin resistance in mycobacterium tuberculosis. | colorimetric methods are cheap, reproducible, and rapid methods of detecting drug resistance in mycobacterium tuberculosis. the mtt (3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide) method is one such technique that has been established in our laboratory to detect rifampicin resistance. the present study compared the results of the mtt method with those of the proportion method and real-time polymerase chain reaction (rtpcr) in order to establish sensitivity and specificity of mtt. ... | 2016 | 22361766 |
| de novo design of potential reca inhibitors using multi objective optimization. | de novo ligand design involves optimization of several ligand properties such as binding affinity, ligand volume, drug likeness, etc. therefore, optimization of these properties independently and simultaneously seems appropriate. in this paper, the ligand design problem is modeled in a multiobjective using archived multiobjective simulated annealing (amosa) as the underlying search algorithm. the multiple objectives considered are the energy components similarity to a known inhibitor and a novel ... | 2016 | 22392725 |
| disseminated tuberculosis presenting as tuberculous peritonitis and sepsis tuberculosa gravissima in a patient with cirrhosis of the liver: a diagnosis of challenge. | we report the case of an 81-year-old man diagnosed with liver cirrhosis complicated by spontaneous bacterial peritonitis and septic shock. mycobacterium tuberculosis complex was isolated from the ascites, sputum, and blood culture 1 month after the patient died. clinicians should be aware of the unusual diagnosis of sepsis tuberculosa gravissima presenting with tuberculous peritonitis, which is easily misdiagnosed as spontaneous bacterial peritonitis and gram-negative bacillus sepsis in patients ... | 2016 | 24530253 |
| the predictive value of gen-probe's amplified mycobacterium tuberculosis direct test compared with culturing in paraffin-embedded lymph node tissue exhibiting granulomatous inflammation and negative acid fast stain. | the diagnosis of granulomatous inflammation with possible tuberculosis (tb) infection in histopathology is often difficult. there is a need for a rapid and reliable diagnostic test. thus, we evaluated the performance of the mycobacterium tuberculosis direct (mtd) test in specimens with granulomatous lymphadenitis and negative acid fast stains. | 2016 | 24602771 |
| tuberculous pyomyositis: a re-emerging entity of many faces. | tuberculosis (tb) has become a global concern due to its increasing incidence, particularly in immunocompromised patients, closely following the migratory patterns of populations. tb pyomyositis is a rare extrapulmonary manifestation of tb. its clinical presentation varies and requires a high degree of suspicion for early diagnosis. we present three patients diagnosed with tb pyomyositis: a 46-year-old man with dermatomyositis (dm) and hepatitis b who presented with fever, muscle weakness, and a ... | 2016 | 24609759 |
| evaluation of a modified direct agar proportion method for testing susceptibility of mycobacterium tuberculosis from mgit samples. | the emergence of resistance to anti-tuberculosis (tb) drugs has become an obstacle to effective tb control. thus, there is an urgent need to identify patients and initiate adequate treatment for drug-resistant cases in a timely manner. the bactec mgit 960 system is well known for its rapid culturing time, and is in widespread use in taiwan. in this study, we evaluated the possibility of replacing the traditional indirect agar proportion method with a modified direct agar proportion method (mdapm ... | 2016 | 24662015 |
| biosynthesis of mycobacterial lipids by polyketide synthases and beyond. | over a decade ago, the analysis of the complete sequence of the genome of the human pathogen mycobacterium tuberculosis revealed an unexpectedly high number of open reading frames encoding proteins with homology to polyketide synthases (pkss). pkss form a large family of fascinating multifunctional enzymes best known for their involvement in the biosynthesis of hundreds of polyketide natural products with diverse biological activities. the surprising polyketide biosynthesis capacity of m. tuberc ... | 2016 | 24625105 |
| establishment of a new immunological method for direct detection of mycobacterium in solution. | tuberculosis (tb) is a crucial health problem. prevention of the disease requires rapid diagnosis. rapid liquid culture systems, nucleic acid amplification tests, and high-performance liquid chromatography (hplc) are among the rapid tests used for detecting mycobacterium species. however, these tests are expensive and require extensive equipment and expertise, which is hardly affordable in resource-poor countries. although direct microscopy is performed routinely as an initial step for detection ... | 2016 | 24767420 |
| development and characterization of spray-dried porous nanoaggregates for pulmonary delivery of anti-tubercular drugs. | tuberculosis, mtb or tubercle bacillus (tb) is a lethal, infectious disease mainly caused by various strains of mycobacteria, usually mycobacterium tuberculosis. in this study, guar gum-based porous nanoaggregates were formulated by precipitation technique with two frontline antitubercular drugs, i.e. isoniazid and rifampicin. the formulations were optimized on the basis of various evaluation parameters such as morphology, density, entrapment efficiency and in vitro drug release. the optimized f ... | 2016 | 24870203 |
| contribution of increased isg15, isgylation and deregulated type i ifn signaling in usp18 mutant mice during the course of bacterial infections. | host genetics has a key role in susceptibility to salmonella typhimurium infection. we previously used n-ethyl-n-nitrosourea (enu) mutagenesis to identify a loss-of-function mutation within the gene ubiquitin-specific peptidase 18 (usp18(ity9)), which confers increased susceptibility to salmonella typhimurium. usp18 functions to regulate type i interferon (ifn) signaling and as a protease to remove isg15 from substrate proteins. usp18(ity9) mice are susceptible to infection with salmonella typhi ... | 2016 | 24807690 |
| [comparison of the data of computed tomography and bacteriological studies in the complicated forms of primary tuberculosis in children and adolescents]. | to establish the causes of bacterial excretion in complicated primary tuberculosis in children and adolescents, by comparing the data of computed tomography and the results of bacteriological studies. | 2016 | 25702438 |
| isolated tuberculous liver abscess in an immunocompetent adult patient: a case report and literature review. | tuberculous liver abscess is a rare disease entity even in endemic areas of mycobacterium tuberculosis. it is usually accompanied by pulmonary tuberculosis or enteric tuberculosis. further, an isolated tuberculous liver abscess is extremely rare. the disease is diagnosed by laparotomy or postmortem autopsy in most cases, and some authors adopted a 9-month antituberculosis regimen. we herein report a case of an isolated tuberculous liver abscess that initially manifested as persistent fever and g ... | 2016 | 24231587 |
| current status and future trends in the diagnosis and treatment of drug-susceptible and multidrug-resistant tuberculosis. | the global burden of tuberculosis (tb) is still large. the increasing incidence of drug-resistant, multidrug-resistant (mdr) (resistant to at least rifampicin and isoniazid), and extensively drug-resistant (xdr) (additionally resistant to a fluoroquinolone and kanamycin/amikacin/capreomycin) strains of mycobacterium tuberculosis and the association of active disease with human immunodeficiency virus coinfection pose a major threat to tb control efforts. the rapid detection of m. tuberculosis str ... | 2016 | 24216518 |
| in silico interaction of methyl isocyanate with immune protein responsible for mycobacterium tuberculosis infection using molecular docking. | this article reports in silico analysis of methyl isocyanate (mic) on different key immune proteins against mycobacterium tuberculosis. the analysis shows that mic is released in the bhopal gas tragedy in 1984, which is highly toxic and extremely hazardous to human health. in this study, we have selected immune proteins to perform molecular docking with the help of autodock 4.0. results show that the cd40 ligand and alpha5beta1 integrin have higher inhibition compared to plasminogen activator ur ... | 2016 | 24081639 |
| [economic impact of the introduction of a technique for early detection of mycobacterium tuberculosis complex in clinical samples in a spanish hospital]. | tuberculosis (tb) remains an important public health problem worldwide. our objective was to estimate the economic and health care impact of the introduction of an early diagnosis tecnique of tb, the xpert mtb/rif® assay (xpert). | 2016 | 24100779 |
| smelling the diagnosis: a review on the use of scent in diagnosing disease. | for centuries, our sense of smell has been used as a diagnostic tool in the practice of medicine, be it for recognising gas gangrene on the battle field or diabetic ketoacidosis in the emergency room. in recent decades, many scent detection studies have been performed with human, animal and electronic noses. the ability of humans to diagnose disease by smelling has only rarely been the subject of quantitative studies. scent detection by animals, on the other hand, has been addressed in several d ... | 2016 | 23956311 |
| discrimination of single base mismatched oligonucleotides related to the rpob gene of mycobacterium tuberculosis using a surface plasmon resonance biosensor. | single base mismatched oligonucleotides related to the rpob gene of mycobacterium tuberculosis, the mutations of which cause drug resistance of the infectious agent, were detected and discriminated using a surface plasmon resonance biosensor system. thiol-modified oligonucleotides of the selected sequence (the probe) and 1-mercapto-6-hexanol were immobilized on a gold sensor surface. hybridization between immobilized probe p2 and perfectly matched target t2 as well as a single base mismatched ta ... | 2016 | 23654350 |
| calvarial tuberculosis with parenchymal involvement. | tuberculosis is an infectious disease caused by the acid-fast bacilli mycobacterium tuberculosis that usually affects the lungs although it may affect any organ. tuberculous osteitis of the cranial bones is an uncommon manifestation of disseminated tuberculosis known as calvarial tuberculosis. cases with parenchymal involvement are infrequent in the literature. we report a case of a patient with multiple osteolytic cranial lesions and a tuberculoma. | 2016 | 23267716 |
| [the role of imaging in thoracic tuberculosis]. | tuberculosis is an infectious disease mostly due to mycobacterium tuberculosis. it is frequent in developing countries and its incidence is rising in developed countries. lungs are the most involved organs of the chest but other structures can be affected. imaging is fundamental in the management of the disease. confirmation of diagnosis can be made only by bacteriologic and/or histologic exams. the first approach of diagnosis is based on clinical symptoms and chest x-ray signs. radiologic signs ... | 2016 | 24874403 |
| development of sandwich-form biosensor to detect mycobacterium tuberculosis complex in clinical sputum specimens. | mycobacterium tuberculosis, the causing agent of tuberculosis, comes second only after hiv on the list of infectious agents slaughtering many worldwide. due to the limitations behind the conventional detection methods, it is therefore critical to develop new sensitive sensing systems capable of quick detection of the infectious agent. in the present study, the surface modified cadmium-telluride quantum dots and gold nanoparticles conjunct with two specific oligonucleotides against early secretor ... | 2016 | 25181404 |
| additive effect of recombinant mycobacterium tuberculosis esat-6 protein and esat-6/cfp-10 fusion protein in adhesion of macrophages through fibronectin receptors. | tuberculous granulomas are the sites of interaction between the t cells, macrophages, and extracellular matrix (ecm) to control the infection caused by mycobacterium tuberculosis (m. tuberculosis). a predominant role of rd-1-encoded secretory proteins, early secreted antigenic target-6 (esat-6), and culture filtrate protein-10 (cfp-10) in the formation of granulomas has recently been emphasized. however, the precise molecular events that induce the formation of these granulomatous structures are ... | 2016 | 25081983 |
| a review of emerging trends in the treatment of tuberculosis. | this review attempts to summarize the information available on emerging trends in the treatment of tuberculosis caused by the bacteria mycobacterium tuberculosis. nanostructured biomaterials, liposomes, microparticles and solid lipid nanoparticles have unique physicochemical properties such as particularly small and convenient size, sustained release, great surface area to mass ratio and high reactivity with structure. these properties can be useful in easing the administration of antimicrobial ... | 2016 | 25365354 |
| [implementation of a telediagnostic system for tuberculosis and determination of multi-drug resistance based in the mods method in trujillo, peru]. | to implement a system for remote diagnosis of tuberculosis and multidrug resistance (mdr) using the microscopic-observation drug susceptibility assay (mods) method in the mycobacteria laboratory, trujillo center of excellence in tuberculosis (cenex-trujillo). the system included a variant of an algorithm for recognition of mycobacterium tuberculosis recently reported from digital images of mods cultures of sputum samples. | 2016 | 25418641 |
| [impact of the migratory movements in the bacterial resistance to antibiotics]. | spain is among the main receptor countries for immigration; but there are few studies available which target antibiotic-resistance in immigrants. our objective was to review the current knowledge on the impact of antibiotic resistance in immigrants compared with the prevalence in the autochthonous population. | 2016 | 25418572 |
| mucosal vaccination against tuberculosis using ag85a-loaded immunostimulating complexes. | tuberculosis (tb) is one of the major devastating diseases in the world, mainly caused by mycobacterium tuberculosis. furthermore, multi-drug resistant tb and extremely drug resistant tb are becoming big problems globally. bacillus calmette-guerin (bcg) is the only available vaccine which provides protection against tb. the bcg vaccine is effective in children but not recommended in adults and elderly patients due to an associated low risk of infection with mycobacterium tuberculosis and variabl ... | 2016 | 25307269 |
| comparison of cyp141 and is6110 for detection of mycobacterium tuberculosis from clinical specimens by pcr. | tuberculosis is a major public health problem throughout the world. tb's worldwide patterns of prevalence coupled with the increase in incidence of hiv infection threaten the health and lives of humans worldwide. rapid detection of tb and the rapidly initiation of the administration of medication are important strategies for stopping the transmission of this disease transmission and its resistance to anti-tb drugs. molecular methods are advantageous relative to conventional techniques due to the ... | 2016 | 25304426 |
| rapid screening of rpob and katg mutations in mycobacterium tuberculosis isolates by high-resolution melting curve analysis. | early detection of multidrug-resistant tuberculosis (mdr-tb) is essential to prevent its transmission in the community and initiate effective anti-tb treatment regimen. | 2016 | 25297024 |
| [immune reconstitution inflammatory syndrome related with infliximab interruption in patient with crohn's disease and active tuberculosis]. | tumor necrosis factor alpha inhibitors are associated with an increased risk of active tuberculosis. however, its interruption in this setting may trigger a paradoxical response to tuberculosis treatment, as an immune reconstitution inflammatory syndrome. we present the case of a 36-year-old patient, with crohn's disease, treated with infliximab for the last 8 years, who was admitted with miliary tuberculosis. a pan-susceptible mycobacterium tuberculosis strain was isolated. infliximab was inter ... | 2016 | 25298330 |
| [characteristics of lung foci revealed by computed tomography in high-risk children and adolescents for tuberculosis]. | to give an x-ray pattern of single lung foci revealed by computed tomography (ct) in children and adolescents examined for tuberculosis. | 2016 | 25276882 |
| the effects of sp110's associated genes on fresh cavitary pulmonary tuberculosis in han chinese population. | sp110 is a promising anti-mycobacterium tuberculosis (mtb) gene. to investigate the effects of sp110 and its associated genes, i.e., mybbp1a and rela, on pathological progression of mtb infection, an association study with 424 patients of fresh pulmonary tuberculosis (ptb) and 424 healthy controls was performed. moreover, classification and regression tree and multifactor dimensionality reduction were employed to explore the effects of gene-gene interactions on cavitary ptb. the results indicate ... | 2016 | 25612917 |
| head and neck tuberculosis: 6-year retrospective study. | pulmonary involvement exists in 80% of cases of infection with mycobacterium tuberculosis; however, in up to 20% of cases there may be extra-pulmonary involvement. in the ent area, the most common site is cervical lymphadenitis, affecting approximately 95% of cases. | 2016 | 25630666 |
| microrna-124 inhibits the progression of adjuvant-induced arthritis in rats. | micrornas (mirnas) are small endogenous, non-coding rnas that act as post-transcriptional regulators. we analysed the in vivo effect of mirna-124 (mir-124, the rat analogue of human mir-124a) on adjuvant-induced arthritis (aia) in rats. | 2016 | 25596157 |
| pharmacoinformatics analysis to identify inhibitors of mtb-asadh. | aspartate-semialdehyde dehydrogenase (asadh; ec 1.2.1.11) is a key enzyme in the biosynthesis of essential amino acids in prokaryotes and fungi, inhibition of asadh leads to the development of novel antitubercular agents. in the present work, a combined structure and ligand-based pharmacophore modeling, molecular docking, and molecular dynamics (md) approaches were employed to identify potent inhibitors of mycobacterium tuberculosis (mtb)-asadh. the structure-based pharmacophore hypothesis consi ... | 2016 | 25565373 |
| intracellular esat-6: a new biomarker for mycobacterium tuberculosis infection. | early secreted antigenic target 6 (esat-6) is a virulent factor of mycobacterium tuberculosis (mtb). the identification of intracellular (i/c) esat-6 in host cells would be a direct marker of mtb infection. we developed a method to detect i/cesat-6 by flow cytometry. the aim of this study is to investigate the expression of i/cesat-6 in the host cells of individuals with mtb infection. | 2016 | 25545352 |
| tuberculosis of the manubriosternal joint and concurrent asymptomatic active pulmonary tuberculosis in a patient presenting with a chest wall mass. | a 62-year-old woman presented to our hospital with an anterior chest wall swelling. computed tomography (ct) and magnetic resonance imaging showed findings consistent with an infectious arthritis of the manubriosternal joint, and ct images also demonstrated multiple centrilobular nodules in both lungs, suggesting an infectious bronchiolitis. a ct-guided fine needle aspiration of a presternal mass yielded growth of mycobacterium tuberculosis. bronchoalveolar lavage confirmed an active pulmonary t ... | 2016 | 25457526 |
| mechanism of esat-6 membrane interaction and its roles in pathogenesis of mycobacterium tuberculosis. | the 6-kda early secreted antigenic target (esat-6; esxa) of mycobacterium tuberculosis was first identified as a potent t-cell antigen, and it is now recognized as a pore-forming toxin that is essential for virulence of m. tuberculosis. esat-6 is secreted through the esx-1 secretion system (type vii) of m. tuberculosis and has been implicated to mediate mycobacterial cytosolic translocation within the host macrophages by rupturing the phagosomal membranes. recent studies have made significant pr ... | 2016 | 26456678 |
| tissue factor expression by myeloid cells contributes to protective immune response against mycobacterium tuberculosis infection. | tissue factor (tf) is a transmembrane glycoprotein that plays an essential role in hemostasis by activating coagulation. tf is also expressed by monocytes/macrophages as part of the innate immune response to infections. in the current study, we determined the role of tf expressed by myeloid cells during mycobacterium tuberculosis (m. tb) infection by using mice lacking the tf gene in myeloid cells (tf(δ) ) and human monocyte derived macrophages (mdms). we found that during m. tb infection, a def ... | 2016 | 26471500 |
| erratum to: discovery of new mycobacterium tuberculosis proteasome inhibitors using a knowledge-based computational screening approach. | 2016 | 26498122 | |
| efflux pump inhibitors: targeting mycobacterial efflux systems to enhance tb therapy. | the emergence of drug resistance continues to plague tb control, with a global increase in the prevalence of mdr-tb. this acts as a gateway to xdr-tb and thus emphasizes the urgency for drug development and optimal treatment options. bedaquiline is the first new anti-tb drug approved by the fda in 40 years and has been shown to be an effective treatment option for mdr mycobacterium tuberculosis infection. bedaquiline has also recently been included in clinical trials for new regimens with the ai ... | 2016 | 26472768 |
| lower cytotoxicity, high stability, and long-term antibacterial activity of a poly(methacrylic acid)/isoniazid/rifampin nanogel against multidrug-resistant intestinal mycobacterium tuberculosis. | to overcome the undesirable side effects and reduce the cytotoxicity of isoniazid (inh) and rifampin (rmp) in the digestive tract, a poly(methacrylic acid) (pmaa) nanogel was developed as a carrier of inh and rmp. this pmaa/inh/rmp nanogel was prepared as a treatment for intestinal tuberculosis caused by multidrug-resistant mycobacterium tuberculosis (mtb). the morphology, size, and in vitro release properties were evaluated in a simulated gastrointestinal medium, and long-term antibacterial per ... | 2016 | 26478357 |
| synthesis and characterization of the antitubercular phenazine lapazine and development of plga and pcl nanoparticles for its entrapment. | the aim of this work was to develop and characterize nanoparticles as carriers of lapazine, a phenazine derived from β-lapachone; its antimycobacterial activity is described for the first time as a potential treatment for tuberculosis. the lapazine was synthesized, and by using gas chromatography coupled to a flame ionization detector, it was possible to evaluate its purity degree of almost 100%. for better elucidation of the molecular structure, mass spectroscopy and 1h nmr were carried out and ... | 2016 | 26478333 |
| multicenter evaluation of anyplex plus mtb/ntm mdr-tb assay for rapid detection of mycobacterium tuberculosis complex and multidrug-resistant isolates in pulmonary and extrapulmonary specimens. | the rapid diagnosis of tuberculosis (tb) and the detection of drug-resistant mycobacterium tuberculosis strains are critical for successful public health interventions. therefore, tb diagnosis requires the availability of diagnostic tools that allow the rapid detection of m. tuberculosis and drug resistance in clinical samples. here, we performed a multicenter study to evaluate the performance of the seegene anyplex mtb/ntm mdr-tb assay, a new molecular method based on a multiplex real-time pcr ... | 2016 | 26491178 |
| prevalence of mutations conferring resistance among multi- and extensively drug-resistant mycobacterium tuberculosis isolates in china. | to identify the mutations in multi- and extensively drug-resistant tuberculosis isolates and to evaluate the use of molecular markers of resistance, we analyzed 257 multi- and extensively drug-resistant isolates and 64 pan-sensitive isolates from 23 provinces in china. seven loci associated with drug resistance, including rpob for rifampin (rif), katg, inha and oxyr-ahpc for isoniazid (inh), gyra and gyrb for ofloxacin (ofx), and rrs for kanmycin (kan), were examined by dna sequencing. compared ... | 2016 | 26486879 |
| impaired cytokine but enhanced cytotoxic marker expression in mycobacterium tuberculosis-induced cd8+ t cells in individuals with type 2 diabetes and latent mycobacterium tuberculosis infection. | type 2 diabetes mellitus (dm) is a risk factor for tuberculosis among individuals with latent mycobacterium tuberculosis infection. to explore the influence of dm on cd8(+) t-cell responses during latent m. tuberculosis infection, we estimated the cytokine and cytotoxic marker expression pattern in individuals with latent m. tuberculosis infection with dm and those with latent m. tuberculosis infection without dm. among individuals with latent m. tuberculosis infection, those with dm had diminis ... | 2016 | 26486635 |
| optimization of inhibitors of mycobacterium tuberculosis pantothenate synthetase based on group efficiency analysis. | ligand efficiency has proven to be a valuable concept for optimization of leads in the early stages of drug design. taking this one step further, group efficiency (ge) evaluates the binding efficiency of each appendage of a molecule, further fine-tuning the drug design process. here, ge analysis is used to systematically improve the potency of inhibitors of mycobacterium tuberculosis pantothenate synthetase, an important target in tuberculosis therapy. binding efficiencies were found to be distr ... | 2016 | 26486566 |
| pulmonary but not subcutaneous delivery of bcg vaccine confers protection to tuberculosis-susceptible mice by an interleukin 17-dependent mechanism. | some of the most promising novel tuberculosis vaccine strategies currently under development are based on respiratory vaccination, mimicking the natural route of infection. in this work, we have compared pulmonary and subcutaneous delivery of bcg vaccine in the tuberculosis-susceptible dba/2 mouse strain, a model in which parenterally administered bcg vaccine does not protect against tuberculosis. our data show that intranasally but not subcutaneously administered bcg confers robust protection a ... | 2016 | 26494773 |
| detection of mycobacterium tuberculosis with nested polymerase chain reaction analysis in enucleated eye ball in eales' disease. | nested polymerase chain reaction (npcr) was performed on enucleated eyeball for detection of mycobacterium tuberculosis (m. tb) genome in a patient with eales' disease. pcr analysis in all previous studies has been done mainly using aqueous, vitreous and epiretinal membranes from these patients. paraffin wax embedded tissue section of the enucleated eyeball was analyzed by histopathology and npcr targeting mpb64 gene and is6110 region of m. tb genome. lymphocytic infiltration was seen in the vit ... | 2016 | 26499903 |
| the n-terminal pre-a region of mycobacterium tuberculosis 2/2hbn promotes no-dioxygenase activity. | a unique defense mechanisms by which mycobacterium tuberculosis protects itself from nitrosative stress is based on the o2 -dependent no-dioxygenase (nod) activity of truncated hemoglobin 2/2hbn (mt2/2hbn). the nod activity largely depends on the efficiency of ligand migration to the heme cavity through a two-tunnel (long and short) system; recently, it was also correlated with the presence at the mt2/2hbn n-terminus of a short pre-a region, not conserved in most 2/2hbns, whose deletion results ... | 2016 | 26499089 |
| design, synthesis, and antimycobacterial activity of novel theophylline-7-acetic acid derivatives with amino acid moieties. | the theophylline-7-acetic acid (7-taa) scaffold is a promising novel lead compound for antimycobacterial activity. here, we derive a model for antitubercular activity prediction based on 14 7-taa derivatives with amino acid moieties and their methyl esters. the model is applied to a combinatorial library, consisting of 40 amino acid and methyl ester derivatives of 7-taa. the best three predicted compounds are synthesized and tested against mycobacterium tuberculosis h37rv. all of them are stable ... | 2016 | 26502828 |
| structural insight into dihydrodipicolinate reductase from corybebacterium glutamicum for lysine biosynthesis. | dihydrodipicolinate reductase is an enzyme that converts dihydrodipicolinate to tetrahydrodipicolinate using an nad(p)h cofactor in l-lysine biosynthesis. to increase the understanding of the molecular mechanisms of lysine biosynthesis, we determined the crystal structure of dihydrodipicolinate reductase from corynebacterium glutamicum (cgdapb). cgdapb functions as a tetramer, and each protomer is composed of two domains, an nterminal domain and a c-terminal domain. the n-terminal domain mainly ... | 2016 | 26502738 |
| development and evaluation of chitosan microparticles based dry powder inhalation formulations of rifampicin and rifabutin. | the lung is the primary entry site and target for mycobacterium tuberculosis; more than 80% of the cases reported worldwide are of pulmonary tuberculosis. hence, direct delivery of anti-tubercular drugs to the lung would be beneficial in reducing both, the dose required, as well as the duration of therapy for pulmonary tuberculosis. in the present study, microsphere-based dry powder inhalation systems of the anti-tubercular drugs, rifampicin and rifabutin, were developed and evaluated, with a vi ... | 2016 | 26406162 |
| moxifloxacin use and its association on the diagnosis of pulmonary tuberculosis in an inner city emergency department. | moxifloxacin can be used in the treatment of tuberculosis, its effect on the diagnosis and treatment of pulmonary tuberculosis is not well characterized. | 2016 | 26416134 |
| noninvasive tuberculosis screening in free-living primate populations in gombe national park, tanzania. | recent advances in noninvasive detection methods for mycobacterial infection in primates create new opportunities for exploring the epidemiology of tuberculosis in free-living species. chimpanzees (pan troglodytes schweinfurthii) and baboons (papio anubis) in gombe national park, tanzania, were screened for infection with pathogens of the mycobacterium tuberculosis complex using fecal is6110 pcr; none was positive. this study demonstrates the feasibility of large-scale mycobacterial screening in ... | 2016 | 26419483 |
| a single-centre experience of relaparoscopy in complications of laparoscopic inguinal hernia repair-feasibility and outcomes. | management of complications of laparoscopic inguinal hernia repair remains challenging as well as debatable. relaparoscopy in management of these complications is relatively newer concept. we tried to analyse the feasibility of relaparoscopy (transabdominal preperitoneal approach) in management of complications of laparoscopic inguinal hernia repair. | 2016 | 26423411 |
| construction of a semisynthetic human vh single-domain antibody library and selection of domain antibodies against α-crystalline of mycobacterium tuberculosis. | the use of human variable heavy (vh) domain antibodies has been on the rise due to their small scaffold size and simple folding mechanism. a highly diverse library is largely dependent on the diversity introduced within the complementarity-determining region (cdr) cassettes. here we introduced diversity with the use of a single framework diversifying all three cdrs using tailored codons consisting of degenerate trinucleotides (nnk). the length of the degeneracy in the cdrs was also taken into co ... | 2016 | 26423338 |
| hiv-1 tuberculosis-associated immune reconstitution inflammatory syndrome. | patients co-infected with hiv-1 and tuberculosis (tb) are at risk of developing tb-associated immune reconstitution inflammatory syndrome (tb-iris) following commencement of antiretroviral therapy (art). tb-iris is characterized by transient but severe localized or systemic inflammatory reactions against mycobacterium tuberculosis antigens. here, we review the risk factors and clinical management of tb-iris, as well as the roles played by different aspects of the immune response in contributing ... | 2016 | 26423994 |
| identification of bostrycin derivatives as potential inhibitors of mycobacterium tuberculosis protein tyrosine phosphatase (mptpb). | as a virulence factor secreted into host cells, the mycobacterium tuberculosis protein tyrosine phosphatase (mptpb) mediates the intracellular survival of m. tuberculosis. mptpb has become an attractive target for the development of new anti-tuberculosis drugs. | 2016 | 26434800 |
| pathology and immune reactivity: understanding multidimensionality in pulmonary tuberculosis. | heightened morbidity and mortality in pulmonary tuberculosis (tb) are consequences of complex disease processes triggered by the causative agent, mycobacterium tuberculosis (mtb). mtb modulates inflammation at distinct stages of its intracellular life. recognition and phagocytosis, replication in phagosomes and cytosol escape induce tightly regulated release of cytokines [including interleukin (il)-1, tumor necrosis factor (tnf), il-10], chemokines, lipid mediators, and type i interferons (ifn-i ... | 2016 | 26438324 |
| a color-reaction-based biochip detection assay for rif and inh resistance of clinical mycobacterial specimens. | the widespread occurrence of drug-resistant mycobacterium tuberculosis places importance on the detection of tb (tuberculosis) drug susceptibility. conventional drug susceptibility testing (dst) is a lengthy process. we developed a rapid enzymatic color-reaction-based biochip assay. the process included asymmetric multiplex pcr/templex pcr, biochip hybridization, and an enzymatic color reaction, with specific software for data operating. templex pcr (tem- pcr) was applied to avoid interference b ... | 2016 | 26437946 |
| profiling serum antibodies to mycobacterium tuberculosis proteins in rhesus monkeys with nontuberculous mycobacteria. | recent evidence indicates that the prevalence of diseases caused by nontuberculous mycobacteria (ntm) has been increasing in both human and animals. in this study, antibody profiles of ntm in rhesus monkeys (macaca mulatta) were determined and compared with those of monkeys infected with mycobacterium tuberculosis complex (mtbc). antibodies against 10 m. tuberculosis proteins, purified protein derivative (ppd), and mammalian old tuberculin (mot) were detected in 14 monkeys naturally infected wit ... | 2016 | 26437786 |
| pyrazinamide and pyrazinoic acid derivatives directed to mycobacterial enzymes against tuberculosis. | tuberculosis (tb) is an infectious diseases responsible for thousands of deaths worldwide. due to the use of antimycobacterial drugs, tb prevalence seemed to be controlled, but with the appearance of resistant tuberculosis cases, the concern about the disease had become significant again, as well as the need for new alternatives to tb treatment. since pyrazinamide (pza) is part of the firstline agents in tb treatment, several derivatives of this drug were described, besides pyrazinoic acid (poa) ... | 2016 | 26427385 |
| suppressor of cytokine signaling-1 and chemokine (c-x-c motif) receptor 3 expressions are associated with caseous necrosis in granulomas from patients with tuberculous lymphadenitis. | we investigated the role of suppressor of cytokine signaling-1 (socs1) and socs3 molecules in lymph nodes from tuberculous lymphadenitis patients (lntb). fewer t cells were noted in lntb cases, which also had raised chemokine (c-x-c motif) receptor 3 (cxcr3) levels. in addition, we observed a positive correlation between cxcr3 and socs1 expression. our data suggest that upregulation of socs1 molecules may contribute to the dissemination of mycobacterium tuberculosis from granulomas. | 2016 | 26455487 |
| anti-mycobacterium tuberculosis activity of antituberculosis drugs and amoxicillin/clavulanate combination. | we report the in vitro drugs interaction by the resazurin drugs combination microtiter assay (redca) of amoxicillin (amo)/clavulanate (clav) with isoniazid (inh), ethambutol (emb), and rifampicin (rif) against susceptible and resistant mycobacterium tuberculosis isolates. the addition of amo/clav to classical antituberculosis drugs should be explored as a promising alternative for the treatment of resistant tuberculosis (tb). | 2016 | 26454420 |
| antimycobacterial activity of five efflux pump inhibitors against mycobacterium tuberculosis clinical isolates. | 2016 | 26464012 | |
| multi-center evaluation of a user-friendly lateral flow assay to determine ip-10 and ccl4 levels in blood of tb and non-tb cases in africa. | multi-center evaluation of a user-friendly lateral flow test for detection of ip-10 and ccl4 levels in mycobacterium tuberculosis (mtb) antigen-stimulated whole blood samples from tuberculosis (tb) suspects. | 2016 | 26285074 |
| thermodynamic proxies to compensate for biases in drug discovery methods. | we propose a framework with simple proxies to dissect the relative energy contributions responsible for standard drug discovery binding activity. | 2016 | 26311555 |
| type i, ii, and iii interferons: regulating immunity to mycobacterium tuberculosis infection. | interferons (ifns) are cytokines released by host cells in response to the presence of pathogens or tumor cells. the aim of this review was to present the previously known and new findings about the role of interferons type i and ii, and recently discovered type iii in mycobacterium tuberculosis (m. tuberculosis) infection control. infection of various cell types with m. tuberculosis induce both ifn-α and ifn-β synthesis. the majority of the studies support the findings that ifn type i actually ... | 2016 | 26362801 |
| genotypic assessment of drug-resistant tuberculosis in baghdad and other iraqi provinces using low-cost and low-density dna microarrays. | we report on a molecular investigation carried out to ascertain the prevalence of drug-resistant tuberculosis (tb) and the specific gene mutations responsible for resistance to rifampicin (rif) and/or isoniazid (inh) in iraq. in total, 110 clinical isolates from category ii tb cases from baghdad (58%) and several iraqi provinces (42%) were analysed using colorimetric, low-cost and low-density (lcd) microarrays (myco-direct and myco-resist lcd array kits) to identify the point mutations responsib ... | 2016 | 26555649 |
| excessive cytolytic responses predict tuberculosis relapse after apparently successful treatment. | currently, there are no tools to accurately predict tuberculosis relapse. this study aimed to determine whether patients who experience tuberculosis relapse have different immune responses to mycobacteria in vitro than patients who remain cured for 2 years. | 2016 | 26351358 |
| spray-dried particles as pulmonary delivery system of anti-tubercular drugs: design, optimization, in vitro and in vivo evaluation. | currently, one-third of the world's population is infected with tuberculosis (tb) mainly spread by inhalation of the tubercle bacilli, mycobacterium tuberculosis. patient non-compliance is the major reason for failure of anti-tubercular drugs (atds) chemotherapy due to multidrug administration for longer duration of time period. the main aim of current research study was to develop and characterize inhalable spray-dried particles for pulmonary delivery of atds, i.e., rifampicin (rif) and isoniaz ... | 2016 | 26334961 |
| mechanism of action of the tuberculosis and crohn disease risk factor irgm in autophagy. | polymorphisms in the irgm gene, associated with crohn disease (cd) and tuberculosis, are among the earliest identified examples documenting the role of autophagy in human disease. functional studies have shown that irgm protects against these diseases by modulating autophagy, yet the exact molecular mechanism of irgm's activity has remained unknown. we have recently elucidated irgm's mechanism of action. irgm functions as a platform for assembling, stabilizing, and activating the core autophagic ... | 2016 | 26313894 |
| new lipase assay using pomegranate oil coating in microtiter plates. | lipases play various roles in fat digestion, lipoprotein metabolism, and in the mobilization of fat stored in lipid bodies in animals, plants and microorganisms. in association with these physiological functions, there is an important field of research for discovering lipase inhibitors and developing new treatments of diseases such as obesity, atherosclerosis, diabetes and tuberculosis. in this context, the development of convenient, specific and sensitive analytical methods for the detection an ... | 2016 | 26343557 |
| the mycobacterium tuberculosis ppe protein rv1168c induces stronger b cell response than rv0256c in active tb patients. | tuberculosis (tb) caused by mycobacterium tuberculosis is a serious global health problem and is responsible for millions of deaths every year. for effective control of this dreadful disease, it is necessary to diagnose tb cases at the initial stages of infection. the serodiagnosis of disease represents simple, rapid and inexpensive method that can be used at the primary health care levels. in this study we have compared sensitivity of two ppe proteins of m. tuberculosis, i.e., rv0256c and rv116 ... | 2016 | 26364913 |
| innate immunity in tuberculosis: how the sensing of mycobacteria and tissue damage modulates macrophage death. | the success of mycobacterium tuberculosis as a human pathogen has been attributed to the ability of the bacillus to proliferate inside macrophages and to induce cell death. this review describes how the sensors of the innate immune system modulate the cell death pathways in infected macrophages and, consequently, the pathogenesis of tuberculosis. | 2016 | 26369715 |
| alveolar epithelial cells in mycobacterium tuberculosis infection: active players or innocent bystanders? | tuberculosis (tb) is a disease that kills one person every 18 s. tb remains a global threat due to the emergence of drug-resistant mycobacterium tuberculosis (m.tb) strains and the lack of an efficient vaccine. the ability of m.tb to persist in latency, evade recognition following seroconversion, and establish resistance in vulnerable populations warrants closer examination. past and current research has primarily focused on examination of the role of alveolar macrophages and dendritic cells dur ... | 2016 | 26384325 |
| in vitro antituberculosis activity of diterpenoids from the vietnamese medicinal plant croton tonkinensis. | diterpenoids from the vietnamese medicinal plant croton tonkinensis are rich in ent-kaurane, kaurane and the grayanane class and are valuable intermediate plant metabolites with different bioactivities. in this study, we report the antituberculosis activity of these diterpenoids against both susceptible and resistant strains of m. tuberculosis for the first time. all of the ent-kaurane, kaurane and grayanane diterpenoids showed high to moderate activity against mycobacterium. the highest antitub ... | 2016 | 26386972 |
| cell-peptide specific interaction can inhibit mycobacterium tuberculosis h37rv infection. | studying proteins from the m. tuberculosis h37rv envelop is important for understanding host-pathogen interaction regarding bacterial infection and survival within a host; such knowledge is indispensable regarding studies aimed at developing drugs or vaccines against tuberculosis, a disease which continues to cause more than one million deaths worldwide every year. the present work presents a study of the rv3705c protein which has been described as being an outer protein. several servers and bio ... | 2016 | 26375297 |
| modulation of host ubiquitin system genes in human endometrial cell line infected with mycobacterium tuberculosis. | endometrium is one of the most commonly affected sites in genital tuberculosis. the understanding of its interaction with the tubercle bacilli is of paramount importance for studying the pathogenesis of this disease. the main objective of this work was to study the interplay between mycobacterium tuberculosis and host endometrial epithelial cell lines (ishikawa cell lines), and to identify the differentially expressed genes upon tuberculosis infection. to study this, suppression subtractive hybr ... | 2016 | 26403675 |
| genotypic characteristics of mycobacterium tuberculosis circulating in xinjiang, china. | tuberculosis (tb), a chronic infectious disease caused by mycobacterium tuberculosis (mtb), poses a serious threat to human health. we investigated the genotypes of mtb in the high prevalence province xinjiang, china. | 2016 | 26414438 |
| culture-positive pediatric tuberculosis in toronto, ontario: sources of infection and relationship of birthplace and mycobacterial lineage to phenotype. | few data relate mycobacterium tuberculosis (mtb) lineage and disease phenotype in the pediatric population or examine the contribution of travel to the tuberculosis (tb)-endemic country in north america. we examined clinical, demographic and mtb genotype data from patients with tb who were treated in toronto between 2002 and 2012. | 2016 | 26379168 |
| optimization of a human bacille calmette-guérin challenge model: a tool to evaluate antimycobacterial immunity. | there is an urgent need for an improved tuberculosis vaccine. the lack of a validated correlate of protection slows progress in achieving this goal. a human mycobacterial challenge model, using bacille calmette-guérin (bcg) as a surrogate for a mycobacterium tuberculosis challenge, would facilitate vaccine selection for field efficacy testing. optimization of this model is required. | 2016 | 26450421 |
| case report of a false positive result of the xpert(®) mtb/rif assay for rifampicin resistance in mycobacterium tuberculosis complex. | in the present case, we report a false positive result for the detection of rifampicin (rif) resistance by the xpert(®) mtb/rif assay, version g4.miliary mycobacterium tuberculosis infection (miliary tb) was suspected in a 50-year old angolan woman. imaging of the thorax and abdomen displayed diffuse lesions. the xpert(®) mtb/rif assay conducted on the broncho-alveolar lavage (bal) fluid was positive for tb and positive for rif resistance. confirmatory molecular tests and the phenotypic drug sus ... | 2016 | 26400761 |