Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| macrophage polarization drives granuloma outcome during mycobacterium tuberculosis infection. | mycobacterium tuberculosis, the causative agent of tuberculosis (tb), induces formation of granulomas, structures in which immune cells and bacteria colocalize. macrophages are among the most abundant cell types in granulomas and have been shown to serve as both critical bactericidal cells and targets for m. tuberculosis infection and proliferation throughout the course of infection. very little is known about how these processes are regulated, what controls macrophage microenvironment-specific ... | 2015 | 25368116 |
| evaluation of pyrosequencing for detecting extensively drug-resistant mycobacterium tuberculosis among clinical isolates from four high-burden countries. | reliable molecular diagnostics, which detect specific mutations associated with drug resistance, are promising technologies for the rapid identification and monitoring of drug resistance in mycobacterium tuberculosis isolates. pyrosequencing (psq) has the ability to detect mutations associated with first- and second-line anti-tuberculosis (tb) drugs, with the additional advantage of being rapidly adaptable for the identification of new mutations. the aim of this project was to evaluate the perfo ... | 2015 | 25367911 |
| optimization of a nucleic acid-based reporter system to detect mycobacterium tuberculosis antibiotic sensitivity. | we previously reported the development of a prototype antibiotic sensitivity assay to detect drug-resistant mycobacterium tuberculosis using infection by mycobacteriophage to create a novel nucleic acid transcript, a surrogate marker of mycobacterial viability, detected by reverse transcriptase pcr (m. c. mulvey et al., mbio 3: e00312-11, 2012). this assay detects antibiotic resistance to all drugs, even drugs for which the resistance mechanism is unknown or complex: it is a phenotypic readout u ... | 2015 | 25367910 |
| feasibility of the interferon-gamma enzyme-linked immunospot assay in chronic renal failure patients and immunocompetent subjects: a head-to-head comparison. | diagnosis of latent mycobacterium tuberculosis infection (ltbi) by the tuberculin skin test (tst) is hampered due to anergy and impaired sensitivity in chronic renal failure (crf) patients. we aimed to compare the diagnostic performance of the tst with that of interferon-gamma immunospot (t-spot.tb®) assay in diagnosing ltbi in crf and immunocompetent (ic) patients. a total of 74 crf and 75 ic patients prospectively underwent the tst and t-spot.tb. latent m. tuberculosis infection estimated by t ... | 2015 | 25387208 |
| il12rβ1δtm is a secreted product of il12rb1 that promotes control of extrapulmonary tuberculosis. | il12rb1 is a human gene that is important for resistance to mycobacterium tuberculosis infection. il12rb1 is expressed by multiple leukocyte lineages, and encodes a type i transmembrane protein (il12rβ1) that associates with il12p40 and promotes the development of host-protective t(h)1 cells. recently, we observed that il12rb1—the mouse homolog of il12rb1—is alternatively spliced by leukocytes to produce a second isoform (il12rβ1δtm) that has biological properties distinct from il12rβ1. although ... | 2015 | 25404030 |
| bis-molybdopterin guanine dinucleotide is required for persistence of mycobacterium tuberculosis in guinea pigs. | mycobacterium tuberculosis is able to synthesize molybdopterin cofactor (moco), which is utilized by numerous enzymes that catalyze redox reactions in carbon, nitrogen, and sulfur metabolism. in bacteria, moco is further modified through the activity of a guanylyltransferase, moba, which converts moco to bis-molybdopterin guanine dinucleotide (bis-mgd), a form of the cofactor that is required by the dimethylsulfoxide (dmso) reductase family of enzymes, which includes the nitrate reductase narghi ... | 2015 | 25404027 |
| delamanid: a review of its use in patients with multidrug-resistant tuberculosis. | delamanid (deltyba(®)), a nitroimidazo-oxazole derivative, is a new anti-tuberculosis (tb) drug which exhibits potent in vitro and in vivo antitubercular activity against drug-susceptible and -resistant strains of mycobacterium tuberculosis. it is approved in several countries, including japan and those of the eu, for use as part of an appropriate combination regimen in adults with multidrug-resistant tuberculosis (mdr-tb) when an effective treatment regimen cannot otherwise be composed due to r ... | 2015 | 25404020 |
| radioiodinated dpa-713 imaging correlates with bactericidal activity of tuberculosis treatments in mice. | current tools for monitoring response to tuberculosis treatments have several limitations. noninvasive biomarkers could accelerate tuberculosis drug development and clinical studies, but to date little progress has been made in developing new imaging technologies for this application. in this study, we developed pulmonary single-photon emission computed tomography (spect) using radioiodinated dpa-713 to serially monitor the activity of tuberculosis treatments in live mice, which develop necrotic ... | 2015 | 25403669 |
| clustered regularly interspaced short palindromic repeats (crisprs) analysis of members of the mycobacterium tuberculosis complex. | typical crispr (clustered, regularly interspaced, short palindromic repeat) regions are constituted by short direct repeats (drs), interspersed with similarly sized non-repetitive spacers, derived from transmissible genetic elements, acquired when the cell is challenged with foreign dna. the analysis of the structure, in number and nature, of crispr spacers is a valuable tool for molecular typing since these loci are polymorphic among strains, originating characteristic signatures. the existence ... | 2015 | 25399110 |
| nested and multiplex real-time pcr using dual-labeled probes: detecting and discriminating mycobacterium tuberculosis complex members in cultures and animal tissues. | members of the mycobacterium tuberculosis complex (mtc) are causative agents of tuberculosis (tb) in both humans and animals. in the last two decades, the accumulating knowledge of the nucleotide sequences of several genes, and of the whole genomes, of mtc members has allowed the development of novel molecular assays able to detect and discriminate between these species. however, despite the significant advances in the development of molecular assays for detecting mtc members in human samples, o ... | 2015 | 25399092 |
| synthesis and evaluation of antimycobacterial activity of new benzimidazole aminoesters. | a total of 51 novel benzimidazoles were synthesized by a 4-step reaction starting from basic compound 4-fluoro-3-nitrobenzoic acid under relatively mild reaction conditions. the structure of the novel benzimidazoles was confirmed by mass spectra as well as (1)h nmr spectroscopic data. out of the 51 novel synthesized compounds, 42 of them were screened for their antimycobacterial activity against mycobacterium tuberculosis h37rv strain using bactiter-glo™ microbial cell viability (btg) method. re ... | 2015 | 24996257 |
| real time pcr in childhood tuberculosis: a valuable diagnostic tool. | the present study was conducted to detect and quantitate mycobacterium tuberculosis from various body fluid specimens of cases of tuberculosis by real time pcr technique and compare results with conventional pcr technique and culture. one hundred fifteen children (<18 y) with tuberculosis (diagnosed as per iap guidelines) and 32 disease matched controls from the department of pediatrics, s.n. medical college, agra, were included in the study. different body fluids (csf, gastric aspirate, pleural ... | 2015 | 25029996 |
| highly immunoreactive antibodies against the rhup-f2 fragment (aa 63-161) of the iron-regulated hupb protein of mycobacterium tuberculosis and its potential for the serodiagnosis of extrapulmonary and recurrent tuberculosis. | hupb is an iron-regulated protein in mycobacterium tuberculosis that functions as a positive regulator of mycobactin biosynthesis. it is essential for the growth and survival of the pathogen inside macrophages. previously, using the full-length rhupb of m. tuberculosis, we demonstrated high levels of anti-hupb antibodies in the serum of pulmonary tuberculosis (tb) and, interestingly, extrapulmonary tb patients with negligible levels in household contacts and healthy controls. here, we used three ... | 2015 | 25037869 |
| a close-up on the epidemiology and transmission of multidrug-resistant tuberculosis in poland. | multidrug-resistant tuberculosis (mdr-tb) poses a serious challenge to the global control of the disease. the purpose of this study was to characterize mdr-tb patients from poland and to determine the extent of mdr-tb disease attributable to recent transmission. the study included all 46 patients diagnosed with mdr-tb in poland in 2004 and followed up for 6 years (until 2011). for each patient, sociodemographic and clinical characteristics, treatment outcomes, and bacteriological data were colle ... | 2015 | 25037868 |
| prevalence of gyra and b gene mutations in fluoroquinolone-resistant and -sensitive clinical isolates of mycobacterium tuberculosis and their relationship with mic of ofloxacin. | the study was done to know the prevalent mutations of gyra and gyrb genes, and their significance with drug resistance in clinical isolates of mycobacterium tuberculosis. a total of 100 ofloxacin- (ofx) resistant and 100 ofx-sensitive isolates of m. tuberculosis were consecutively selected from routine tuberculosis laboratory. drug resistance pattern of these isolates was recorded. mic of ofx was tested in all these isolates by absolute concentration method. quinolone resistance determining regi ... | 2015 | 25052485 |
| common polymorphisms in the cd43 gene region are associated with tuberculosis disease and mortality. | cd43, a surface glycoprotein, regulates mycobacterium tuberculosis macrophage binding, replication, and proinflammatory cytokine induction in a murine model. we hypothesized that single-nucleotide polymorphisms (snps) in the cd43 gene region are associated with human tuberculosis (tb) susceptibility. we performed a case-population study in discovery (352 tb cases and 382 control subjects) and validation cohorts (339 tb cases and 376 control subjects). we examined whether 11 haplotype-tagging snp ... | 2015 | 25078322 |
| dynamics differentiate between active and inactive inteins. | the balance between stability and dynamics for active enzymes can be somewhat quantified by studies of intein splicing and cleaving reactions. inteins catalyze the ligation of flanking host exteins while excising themselves. the potential for applications led to engineering of a mini-intein splicing domain, where the homing endonuclease domain of the mycobacterium tuberculosis reca (mtu reca) intein was removed. the remaining domains were linked by several short peptides, but splicing activity i ... | 2015 | 25087201 |
| evaluating indeterminate interferon-γ-release assay results in patients with chronic inflammatory diseases receiving immunosuppressive therapy. | to analyze the rate of indeterminate interferon-γ-release assay (specifically the quantiferon-tb gold in-tube [qft-git]) testing for mycobacterium tuberculosis in patients with chronic inflammatory disease (cid) compared with the general hospital population (gh) and a healthy reference group of hospital employees (hr), and to analyze factors associated with an indeterminate test result. | 2015 | 25186123 |
| mycobacterium tuberculosis secreted proteins as potential biomarkers for the diagnosis of active tuberculosis and latent tuberculosis infection. | the detection of mycobacterium tuberculosis (mtb) specific human antibodies has been an important diagnostic aid in the diagnosis of tuberculosis (tb) cases with smear-negative sputum samples, especially for the screening of high-risk population. this study focused on the analysis and comparison of the four potential mtb-secreted proteins (esat6, cfp10, ag85b, hsp16.3) and the fusion protein ag85b-hsp16.3 as new markers in the serodiagnosis between active tb and latent tb infection (ltbi). | 2015 | 25131423 |
| [epidemiology of tuberculosis]. | tuberculosis is a contagious disease caused by mycobacterium tuberculosis. it represents, according to world health organization (who), one of the most leading causes of death worldwide. with nearly 8 million new cases each year and more than 1 million deaths per year, tuberculosis is still a public health problem. despite of the decrease in incidence, morbidity and mortality remain important partially due to co-infection with human immunodeficiency virus and emergence of resistant bacilli. all ... | 2015 | 25131367 |
| electrochemical impedance immunosensor for sub-picogram level detection of bovine interferon gamma based on cylinder-shaped tio₂ nanorods. | bovine interferon gamma (boifn-γ) released by t cells plays very important roles in early diagnosis of mycobacterium tuberculosis (mtb) infections and control of bovine tuberculosis. in this work, a label-free electrochemical impedance immunosensor is for the first time developed for highly sensitive determination of boifn-γ. cylinder-shaped tio2 nanorods are synthesized by a facile hydrothermal method, and show high surface area and good hydrophicility. the immunosensor is fabricated by the imm ... | 2015 | 25089816 |
| utility of gastric aspirates for diagnosing tuberculosis in children in a low prevalence area: predictors of positive cultures and significance of non-tuberculous mycobacteria. | in countries with low rates of tuberculosis (tb), yields of gastric aspirates (gas) for mycobacterium tuberculosis (mtb) culture are low. the significance of non-tuberculous mycobacteria (ntm) isolated from ga is uncertain. | 2015 | 25101762 |
| th17-related cytokines contribute to recall-like expansion/effector function of hmbpp-specific vγ2vδ2 t cells after mycobacterium tuberculosis infection or vaccination. | whether cytokines can influence the adaptive immune response by antigen-specific γδ t cells during infections or vaccinations remains unknown. we previously demonstrated that, during bcg/mycobacterium tuberculosis (mtb) infections, th17-related cytokines markedly upregulated when phosphoantigen-specific vγ2vδ2 t cells expanded. in this study, we examined the involvement of th17-related cytokines in the recall-like responses of vγ2vδ2 t cells following mtb infection or vaccination against tb. tre ... | 2015 | 25141829 |
| outbreak of primary inoculation tuberculosis in an acupuncture clinic in southeastern china. | outbreak of mycobacterium tuberculosis infections associated with acupuncture has not been reported. thirteen patients with a painful swollen lump were referred to our hospital. the index patient received acupuncture and paraspinal muscular injection at a local acupuncture clinic in april 2011 and was diagnosed with m. tuberculosis 1 month later. from may 2011 to august 2011, 12 more patients with a swollen lump on the nuchal region or in the lower back or the buttocks region were referred to ou ... | 2015 | 25148461 |
| comparative study of iga vh 3 gene usage in healthy tst(-) and tst(+) population exposed to tuberculosis: deep sequencing analysis. | the acquired immune response against tuberculosis is commonly associated with t-cell responses with little known about the role of b cells or antibodies. there have been suggestions that b cells and humoral immunity can modulate the immune response to mycobacterium tuberculosis. however, the mechanisms involving b-cell responses in m. tuberculosis are not fully understood, in particular the antibody gene preferences. we hypothesized that a preferential use of v genes can be seen associated with ... | 2015 | 25158076 |
| microarray analysis of mycobacterium tuberculosis-infected monocytes reveals il26 as a new candidate gene for tuberculosis susceptibility. | differences in the activity of monocytes/macrophages, important target cells of mycobacterium tuberculosis, might influence tuberculosis progression. with the purpose of identifying candidate genes for tuberculosis susceptibility we infected monocytes from both healthy elderly individuals (a tuberculosis susceptibility group) and elderly tuberculosis patients with m. tuberculosis, and performed a microarray experiment. we detected 78 differentially expressed transcripts and confirmed these resul ... | 2015 | 25157980 |
| effectiveness and tuberculosis-related safety profile of interleukin-1 blocking agents in the management of behçet's disease. | behçet's disease (bd) is a multi-systemic disorder of unknown etiology characterized by relapsing oral-genital ulcers, uveitis, and involvement of the articular, gastrointestinal, neurologic, and vascular systems. although the primum movens of this condition remains unknown, a tangled plot combining autoimmune and autoinflammatory pathways has been hypothesized to explain its start and recurrence. in-depth analysis of bd pathogenetic mechanisms, involving dysfunction of multiple proinflammatory ... | 2015 | 25151975 |
| mycobacterium tuberculosis and the search for the impossible truth. | 2015 | 25162965 | |
| a pathway for sexual dimorphism in innate immunity against mycobacterium tuberculosis infection. | 2015 | 25165164 | |
| tuberculosis in laos, who is at risk: the mahouts or their elephants? | summary tuberculosis (tb) in elephants has the potential to infect humans and is an increasing public health concern. lao pdr is one of the last countries where elephants are still used for timber extraction and where they live in close contact with their mahouts. there are 500 animals at work in the country, some interacting with wild herds. although human tb prevalence is known to be high in laos, studies on elephant tb had yet to be undertaken. from january to july 2012, screening was perform ... | 2015 | 25170549 |
| comment on: clinical significance of 2 h plasma concentrations of first-line anti-tuberculosis drugs: a prospective observational study. | 2015 | 25182065 | |
| household air pollution causes dose-dependent inflammation and altered phagocytosis in human macrophages. | three billion people are exposed to household air pollution from biomass fuel use. exposure is associated with higher incidence of pneumonia, and possibly tuberculosis. understanding mechanisms underlying these defects would improve preventive strategies. we used human alveolar macrophages obtained from healthy malawian adults exposed naturally to household air pollution and compared them with human monocyte-derived macrophages exposed in vitro to respirable-sized particulates. cellular inflamma ... | 2015 | 25254931 |
| mycobacterium tuberculosis dysregulates mmp/timp balance to drive rapid cavitation and unrestrained bacterial proliferation. | active tuberculosis (tb) often presents with advanced pulmonary disease, including irreversible lung damage and cavities. cavitary pathology contributes to antibiotic failure, transmission, morbidity and mortality. matrix metalloproteinases (mmps), in particular mmp-1, are implicated in tb pathogenesis. we explored the mechanisms relating mmp/timp imbalance to cavity formation in a modified rabbit model of cavitary tb. our model resulted in consistent progression of consolidation to human-like c ... | 2015 | 25186281 |
| natural killer cells: a coherent model for their functional role in mycobacterium tuberculosis infection. | tuberculosis is still a leading cause of bacterial infection worldwide, with an estimate of over two billion people latently infected with mycobacterium tuberculosis (mtb). a delicate interplay between mtb and the host's innate and acquired immune system can influence the outcome of the infection, which ranges from pathogen elimination to the establishment of a latent infection or a progressive disease. although the host cell-mediated adaptive immune response is of vital importance in the contro ... | 2015 | 25196698 |
| advances in diagnosis and treatment of latent tuberculosis infection. | in the united states, latent tuberculosis infection (ltbi) affects between 10 and 15 million people, of whom 10% may develop active tuberculosis disease. people at increased risk for tuberculosis reactivation include recent immigrants from countries with a high incidence of tuberculosis, children younger than age 5, people who have been infected with mycobacterium tuberculosis within the past 2 years, or people with immunosuppression for a variety of reasons. appropriate diagnosis and treatment ... | 2015 | 25201941 |
| mycobacterium tuberculosis as cause of a cerebral spindle cell tumor in an hiv patient. | 2015 | 25207620 | |
| author's response: bcg and infection with mycobacterium tuberculosis. | 2015 | 25232042 | |
| pulmonary tuberculosis in asian elephants (elephas maximus): histologic lesions with correlation to local immune responses. | although mycobacterium tuberculosis infection is an important health concern for asian elephants (elephas maximus), no studies have evaluated the associated local immune responses or histologic lesions. in primates including humans, latent tuberculosis is distinguished by well-organized granulomas with th1 cytokine expression, whereas active disease is characterized by poorly organized inflammation and local imbalance in th1/th2 cytokines. this study examined archival, formalin-fixed, paraffin-e ... | 2015 | 25228055 |
| heterologous immunological effects of early bcg vaccination in low-birth-weight infants in guinea-bissau: a randomized-controlled trial. | bacillus calmette-guérin (bcg) seems to have beneficial nonspecific effects; early bcg vaccination of low-birth-weight (lbw) newborns reduces neonatal mortality by >40% due to prevention of primarily septicemia and pneumonia. | 2015 | 25210141 |
| [reply to "emergent strains of rifampicin-resistant mycobacterium tuberculosis complex and susceptibility to isoniazid"]. | 2015 | 25212828 | |
| antimycobacterial activity of dna intercalator inhibitors of mycobacterium tuberculosis primase dnag. | owing to the rise in drug resistance in tuberculosis combined with the global spread of its causative pathogen, mycobacterium tuberculosis (mtb), innovative anti mycobacterial agents are urgently needed. recently, we developed a novel primase-pyrophosphatase assay and used it to discover inhibitors of an essential mtb enzyme, primase dnag (mtb dnag), a promising and unexplored potential target for novel antituberculosis chemotherapeutics. doxorubicin, an anthracycline antibiotic used as an antic ... | 2015 | 25248725 |
| classical against molecular-genetic methods for susceptibility testing of antituberculotics. | tuberculosis currently belongs to rare respiratory diseases in slovakia. however, the emergence and spread of multi-drug resistant tuberculosis (mdr-tb) and extensively drug-resistant tuberculosis (xdr-tb) are major challenges for global tuberculosis control, since the treatment of resistant forms creates both medical and financial problems. cultivation methods of diagnosis are time-consuming, many times exceeding the time of the initial phase of tuberculosis treatment. therefore, in the present ... | 2015 | 25252893 |
| gene mutations in mycobacterium tuberculosis: multidrug-resistant tb as an emerging global public health crisis. | against a constant background of established infections, epidemics of new and old infectious diseases periodically emerge, greatly magnifying the global burden of infections. tb poses formidable challenges to the global health at the public health and scientific level by acquiring gene mutation into anti tb drugs specially rifampin and isoniazid which leads resistant to drug regime and treatment forms. our tools to combat mdr (multidrug resistant) tb are dangerously out of date and ineffective. ... | 2015 | 25257261 |
| evaluation of the microscopic observation drug susceptibility assay for the rapid detection of mdr-tb and xdr-tb in china: a prospective multicentre study. | to perform a multicentre study evaluating the performance of the microscopic observation drug susceptibility (mods) assay for the detection of mdr-tb and xdr-tb in high-burden resource-limited settings. | 2015 | 25266071 |
| role of polymerase chain reaction in breast tuberculosis. | breast tuberculosis (tb) is an uncommon condition even in endemic countries. breast abscess is usually seen in young females. pcr helps in such challenging cases to clinch the correct diagnosis. | 2015 | 25267370 |
| significance of mycobacterium tuberculosis antigen expression in cerebrospinal fluid monocytes in diagnosing tuberculous meningitis. | the aim of this study was to investigate the significance of positive expression of mycobacterium tuberculosis, (mtb) antigen in the cerebrospinal fluid (csf) monocytes in diagnosing tuberculous meningitis (tbm). | 2015 | 24943761 |
| resistance to first-line antituberculosis drugs in spain, 2010-2011. retubes study. | the magnitude of current resistance to antituberculosis drugs in spain is unknown. the objective of this study is to describe resistance to first-line antituberculosis drugs and determine the associated factors. | 2015 | 25027066 |
| an overview to understand the role of pe_pgrs family proteins in mycobacterium tuberculosis h37 rv and their potential as new drug targets. | tuberculosis has long been the scourge of humanity, claiming millions of lives. the family of pe_pgrs gene has been attributed to the mycobacterium tuberculosis pathogenesis over the past few decades. the gene of pe_pgrs family proteins are most often clustered in a region of the genome often as overlapping genes and role in cell surface markers, adhesion and invasion of defense cells of the host (macrophage and dendritic cells). the proline-glutamic acid (pe) domain is responsible for the cellu ... | 2015 | 24975480 |
| characterization of phenotypic and genotypic drug resistance patterns of mycobacterium tuberculosis isolates from a city in mexico. | the emergence of multidrug-resistant (mdr) mycobacterium tuberculosis strains has become a worldwide health care problem, making treatment of tuberculosis difficult. the aim of this study was to determine phenotypic resistance and gene mutations associated with mdr of clinical isolates of mycobacterium tuberculosis from guadalajara, mexico. | 2015 | 24953252 |
| [childhood tuberculosis]. | childhood tb is an indication of failing tb control in the community. it allows disease persistence in the population. mortality and morbidity due to tb is high in children. moreover, hiv co-infection and multidrug-resistant diseases are as frequent in children as in adults. infection is more frequent in younger children. disease risk after primary infection is greatest in infants younger than 2 years. in case of exposure, evidence of infection can be obtained using the tuberculin skin test (tst ... | 2015 | 24932504 |
| 3-benzylidene-4-chromanones: a novel cluster of anti-tubercular agents. | in a quest for developing novel anti-tubercular agents, a series of 3-benzylidene-4-chromanones 1a-l were evaluated for growth inhibition of mycobacterium tuberculosis h37rv. three promising compounds 1d, g, j emerged as the lead compounds with the ic50 and ic90 values of less than 1 µg/ml. evaluation of the potent compounds 1d, g, j and k against vero monkey kidney cells revealed that these compounds are far more toxic to m. tuberculosis than to vero cells. structure-activity relationships demo ... | 2015 | 24939095 |
| tuberculosis of the cuboid: a case report and review of the literature. | tuberculosis (tb) remains a major global health problem. according to a recent world health organization report, it ranks as the second leading cause of death from an infectious disease worldwide. according to the "uk health protection agency tuberculosis in the uk: 2012 report," 8963 cases were reported in the united kingdom in 2011. london had the greatest proportion of cases in the united kingdom and the greatest rate of disease. tb affecting the foot is rare, affecting about 10% of all skele ... | 2015 | 24942371 |
| molecular epidemiology of tuberculosis in cambodian children. | summary we analysed mycobacterium tuberculosis strains from children, hospitalized from january 2004 to july 2008 in the largest paediatric hospital complex in cambodia. specimens were tested for drug susceptibility and genotypes. from the 260 children, 161 strains were available. the east african-indian genotype family was the most common (59.0%), increasing in frequency with distance from the phnom penh area, while the frequency of the beijing genotype family strains decreased. the drug resist ... | 2015 | 25050615 |
| totally drug-resistant tuberculosis and adjunct therapies. | the first cases of totally drug-resistant (tdr) tuberculosis (tb) were reported in italy 10 years ago; more recently, cases have also been reported in iran, india and south africa. although there is no consensus on terminology, it is most commonly described as 'resistance to all first- and second-line drugs used to treat tb'. mycobacterium tuberculosis (m.tb) acquires drug resistance mutations in a sequential fashion under suboptimal drug pressure due to monotherapy, inadequate dosing, treatment ... | 2015 | 24809736 |
| use of amplified mycobacterium tuberculosis direct test in respiratory samples from hiv-infected patients in brazil. | to compare the accuracy of the amplified mycobacterium tuberculosis direct (amtd) test with reference methods for the laboratory diagnosis of tuberculosis in hiv-infected patients. | 2015 | 24831399 |
| shape-based virtual screening, docking, and molecular dynamics simulations to identify mtb-asadh inhibitors. | aspartate β-semialdehyde dehydrogenase (asadh) is a key enzyme for the biosynthesis of essential amino acids and several important metabolites in microbes. inhibition of asadh enzyme is a promising drug target strategy against mycobacterium tuberculosis (mtb). in this work, in silico approach was used to identify potent inhibitors of mtb-asadh. aspartyl β-difluorophosphonate (β-afp), a known lead compound, was used to understand the molecular recognition interactions (using molecular docking and ... | 2015 | 24875451 |
| trends of anti-tuberculosis drug resistance pattern in new cases and previously treated cases of extrapulmonary tuberculosis cases in referral hospitals in northern india. | drug-resistant tuberculosis is one of major current challenges to global public health. the transmission of resistant strains is increasing as a burden of multidrug-resistant tuberculosis (mdr-tb) patients in extra pulmonary tuberculosis (eptb) cases in india. | 2015 | 23023350 |
| mycobacterium tuberculosis, how long did you walk? | 2015 | 23643639 | |
| [emergent strains of rifampicin-resistant mycobacterium tuberculosis complex and susceptibility to isoniazid]. | 2015 | 23623580 | |
| performance comparison between the mycobacteria growth indicator tube system and löwenstein-jensen medium in the routine detection of mycobacterium tuberculosis at public health care facilities in rio de janeiro, brazil: preliminary results of a pragmatic clinical trial. | in view of the fact that the world health organization has recommended the use of the mycobacteria growth indicator tube (mgit) 960 system for the diagnosis of tuberculosis and that there is as yet no evidence regarding the clinical impact of its use in health care systems, we conducted a pragmatic clinical trial to evaluate the clinical performance and cost-effectiveness of the use of mgit 960 at two health care facilities in the city of rio de janeiro, brazil, where the incidence of tuberculos ... | 2015 | 23857683 |
| rapid detection of drug resistance and mutational patterns of extensively drug-resistant strains by a novel genotype® mtbdrsl assay. | the emergence of extensively drug-resistant tuberculosis (xdr-tb) is a major concern in the india. the burden of xdr-tb is increasing due to inadequate monitoring, lack of proper diagnosis, and treatment. the genotype ® mycobacterium tuberculosis drug resistance second line (mtbdrsl) assay is a novel line probe assay used for the rapid detection of mutational patterns conferring resistance to xdr-tb. | 2015 | 24029194 |
| current status of multidrug resistant tuberculosis in a tertiary care hospital of east delhi. | multidrug resistant tuberculosis (mdr-tb) is caused by infection due to mycobacterium tuberculosis which is resistant to both isoniazid (inh) and rifampicin (rif). it is caused by selection of resistant mutant strains due to inadequate treatment and poor compliance. mdr-tb is a major public health problem as the treatment is complicated, cure rates are well below those for drug susceptible tuberculosis and patient remains infectious for months despite receiving the best available therapy. the dr ... | 2015 | 24029192 |
| correction to reinterpreting the mechanism of inhibition of mycobacterium tuberculosis d-alanine:d-alanine ligase by d-cycloserine. | 2015 | 26539621 | |
| cryptococcosis, silicosis, and tuberculous pseudotumor in the same pulmonary lobe. | tuberculosis and cryptococcosis are infectious diseases that can result in the formation of single or multiple nodules in immunocompetent patients. exposure to silica is known to raise the risk of infection with mycobacterium tuberculosis. we report the case of an elderly man with no history of opportunistic infections and no clinical evidence of immunodeficiency but with a six-month history of dry cough and nocturnal wheezing. a chest x-ray revealed a mass measuring 5.0 × 3.5 cm in the right up ... | 2015 | 24310636 |
| the mutations of katg and inha genes of isoniazid-resistant mycobacterium tuberculosis isolates in taiwan. | the isoniazid (inh) resistance of mycobacterium tuberculosis is caused by mutations in the katg and inha genes encoding for catalase-peroxidase and inha, respectively. sequences of the katg and inha gene of 70 isolates were analyzed to identify the mutations and to compare the mutations with their related susceptibilities. | 2015 | 24184004 |
| mobility and temperature resistance of trehalose mycolates as key characteristics of the outer membrane of mycobacterium tuberculosis. | trehalose mycolates are fundamental characteristics of the outer membrane (mycomembrane) of mycobacterium tuberculosis and they are supposed to play a key role in the low permeability and high resistance of mycobacteria to many antibiotics; however, still, the molecular characteristics making mycolates so effective in their biological function are not fully understood. this work aims to investigate by quasi-elastic neutron scattering the diffusive dynamical properties of trehalose mycolates in w ... | 2015 | 24601825 |
| [synthesis and biological properties of α-thymidine 5'-aryl phosphonates]. | the interaction of cdi-activated diethyl phosphonoacetate with methyl 4-aminobenzoat or 3,5-difluoromethylphenylamine followed by treatment with me3sibr in dmf led to n-aryl aminocarbonylmethyl phosphonates and their ethyl esters. their coupling with 3'-acetyl-α-thymidine followed by removal of the acetyl groups gave (α-d-thymidine-5'-il) n-[4-(methoxycarbonyl-, aminocarbonyl- and carboxy)phenyl]-aminocarbonylmethyl phosphonates, (α-d-thymidine-5'-il)-[3,5-bis(trifluoromethyl)phenylaminocarbonyl ... | 2015 | 25696933 |
| evaluation of als assay of tb-sa for diagnosis of pulmonary tuberculosis. | the study evaluated tuberculosis specific antigen (tb-sa) antibody in lymphocyte supernatant (als) assay for diagnosis of pulmonary tuberculosis (ptb). the mean concentration of tb-sa als in ptb patients was significantly higher than that of two control groups (non-ptb patients and healthy subjects, p < 0.001). the area under the curve of the als assay were 0.960 (0.904-0.988, ptb vs healthy subjects), and were 0.822 (0.739-0.887, ptb vs non-ptb patients), respectively. our data indicated the al ... | 2015 | 24702118 |
| inflammation and tuberculosis: host-directed therapies. | tuberculosis (tb) is an airborne infectious disease that kills almost two million individuals every year. multidrug-resistant (mdr) tb is caused by strains of mycobacterium tuberculosis (m. tb) resistant to isoniazid and rifampin, the backbone of first-line antitubercular treatment. mdr tb affects an estimated 500,000 new patients annually. genetic analysis of drug-resistant mdr-tb showed that airborne transmission of undetected and untreated strains played a major role in disease outbreaks. the ... | 2015 | 24717092 |
| comparison of three molecular assays for the detection of rifampin resistance in mycobacterium tuberculosis. | rifampin (rif) is the most important first-line antituberculosis drug, and resistance to this drug may result in treatment failures. we evaluated the diagnostic performances of recently introduced, molecular assays for the detection of rif resistance. | 2015 | 24797357 |
| recombinant tuberculosis vaccine aec/bc02 induces antigen-specific cellular responses in mice and protects guinea pigs in a model of latent infection. | to preliminarily evaluate the immunogenicity and efficacy of the recombinant tuberculosis vaccine aec/bc02 in which ag85b and fusion protein esat6-cfp10 were combined with bacillus calmette-guérin cpg and an aluminum salt-based adjuvant system. | 2015 | 24863497 |
| pulmonary tuberculosis combined with hepatic tuberculosis: a case report and literature review. | tuberculosis, a chronic infectious disease caused by mycobacterium tuberculosis, may invade all organs but mainly affect lungs. most hepatic tuberculosis could be a part of systemic miliary tuberculosis. | 2015 | 24865423 |
| facing multi-drug resistant tuberculosis. | multi-drug resistant tuberculosis (mdr-tb) is caused by mycobacterium tuberculosis strains resistant to at least two of the most effective anti-tuberculosis drugs (i.e., isoniazid and rifampicin). therapeutic regimens based on second- and third-line anti-tuberculosis medicines showed poor efficacy, safety, and tolerability profiles. it was estimated that in 2012 the multi-drug resistant tuberculosis incidence ranged from 300,000 to 600,000 cases, mainly diagnosed in the eastern european and cent ... | 2015 | 24792579 |
| expression kinetics of metalloproteinases and their tissue inhibitors in experimental murine pulmonary tuberculosis. | explore the temporal expression of metalloproteinases (mmps) and tissue inhibitors of metalloproteinases (timps) during experimental tuberculosis induced by virulent mycobacterium tuberculosis strain h37rv. | 2015 | 25275921 |
| immune responses to latent tuberculosis antigen rv2659c in chinese populations. | the latency-associated antigen rv2659c is a starvation-related protein of mycobacterium tuberculosis (m. tuberculosis). it has potential use in tuberculosis (tb) control, but its immunological characteristics in chinese populations are unclear. | 2015 | 24767418 |
| optimisation of the microplate resazurin assay for screening and bioassay-guided fractionation of phytochemical extracts against mycobacterium tuberculosis. | because of increased resistance to current drugs, there is an urgent need to discover new anti-mycobacterial compounds for the development of novel anti-tuberculosis drugs. the microplate resazurin assay (mra) is commonly used to evaluate natural products and synthetic compounds for anti-mycobacterial activity. however, the assay can be problematic and unreliable when screening methanolic phytochemical extracts. | 2015 | 24733665 |
| there is no correlation between sublineages and drug resistance of mycobacterium tuberculosis beijing/w lineage clinical isolates in xinjiang, china. | the beijing/w lineage strains are the major prevalent strains in china. the prevalence, mortality and drug-resistant rates of tuberculosis in xinjiang, northwestern china are higher than in other parts of the country. our previous study results showed that the dominant strains of mycobacterium tuberculosis (mtb) were 'beijing/w lineage' mtb in xinjiang; those strains had no significant correlation with drug resistance. we investigated whether the prevalence of 'beijing/w lineage' sublineage stra ... | 2015 | 24667051 |
| mycobacterium tuberculosis promotes arthritis development through toll-like receptor 2. | rheumatoid arthritis (ra) is a multifactorial disease caused by genetic and environmental factors: however, precise molecular mechanisms underlying its pathogenesis remain largely unknown. treatment of ra patients with disease-modifying biological agents occasionally promotes mycobacterium tuberculosis infection or recurrence of m. tuberculosis, although how infection promotes arthritis has not been characterized. here, we found that arthritis phenotypes in a collagen-induced mouse model were ev ... | 2015 | 24633489 |
| innominate artery injury from disseminated tuberculosis. | a 49-year-old man presented with chest pain and was found to have hemorrhage and drainage from a chest wound secondary to disseminated tuberculosis involving the sternum and ankle. he then developed acute hemorrhage from an innominate artery pseudoaneurysm originating just below a severely diseased sternoclavicular junction. a staged approach was used to manage his pathology given the life-threatening bleeding and his debilitated condition. he underwent endovascular stent grafting to exclude the ... | 2015 | 24585307 |
| challenges and solutions for a rational vaccine design for tb-endemic regions. | vaccines have been successful for global eradication or control of dreaded diseases such as smallpox, diphtheria, tetanus, yellow fever, whooping cough, polio, and measles. unfortunately, this success has not been achieved for controlling tuberculosis (tb) worldwide. bacillus calmette guérin (bcg) is the only available vaccine against tb. paradoxically, bcg has deciphered success in the western world but has failed in tb-endemic areas. in this article, we highlight and discuss the aspects of imm ... | 2015 | 24495096 |
| microbiology and outcomes of peritonitis in northern india. | peritoneal dialysis (pd) is an established treatment modality for end-stage renal disease (esrd). peritonitis remains a serious complication in pd patients and an important cause of drop-out from the program. types of pathogens and their drug resistance patterns may determine the outcome of peritonitis. the present study was undertaken to determine the microbiology of peritonitis in pd patients, antibiotic resistance in commonly isolated bacterial pathogens and clinical outcomes. | 2015 | 24584592 |
| empyema necessitatis due to mycobacterium tuberculosis. | 2015 | 24560698 | |
| vanadium complexes with hydrazone or thiosemicarbazone ligands as potential anti-mycobacterium tuberculosis agents. | tuberculosis (tb) is an infectious disease caused mainly by mycobacterium tuberculosis (mtb) and still an important public health problem worldwide. some factors like the emergence of multidrug resistant (mdr) and extensively drug-resistant (xdr) strains make urgent the research of new active compounds. searching for new inorganic compounds against tb, three new dioxovanadium(v) complexes were obtained upon reaction of [vo(acac)2] with hydrazone and thiosemicarbazone ligands derived from di-2-py ... | 2015 | 24433444 |
| drug resistance to mycobacterium tuberculosis: from the traditional chinese view to modern systems biology. | the pathogen, mycobacterium tuberculosis (m. tuberculosis) is a well-evolved, organized pathogen that has developed drug resistance, specifically multidrug resistance (mdr) and extensive drug resistance (xdr). this review primarily summarizes the mechanisms of drug resistance by m. tuberculosis according to the traditional chinese view. the traditional chinese view of drug resistance includes: the physical barrier of the cell wall; mutations relating to current anti-tb agents; drug efflux pumps; ... | 2015 | 24433008 |
| 3d-qsar studies and shape based virtual screening for identification of novel hits to inhibit mbta in mycobacterium tuberculosis. | mycobacterium tuberculosis, the pathogen responsible for tuberculosis, uses various strategies to survive in a variety of host lesions. the re-emergence of multi-drug-resistant strains of m. tuberculosis underlines the necessity to discover new molecules. inhibitors of aryl acid adenylating enzyme, mbta, involved in siderophore biosynthesis in m. tuberculosis, are being explored as potential anti tubercular agents. in this study, we have used 3d-qsar models and shape based virtual screening to i ... | 2015 | 24417439 |
| [combined determination of spontaneous and antigen-induced production of cytokines for differential diagnostics of active tuberculosis of lungs and latent tuberculosis infection]. | choice of informative biomarkers for diagnostics of mycobacterium tuberculosis infection and differential diagnostics of active tuberculosis (tb) of lungs and latent tuberculosis infection (ltbi). | 2015 | 24341221 |
| multidrug resistant tuberculosis diagnosed by synovial fluid analysis. | tuberculosis remains a major public health problem worldwide. hiv co-infection is contributing to an increased incidence of the disease, particularly that caused by multidrug resistant strains of mycobacterium tuberculosis (mt). we describe an hiv-infected patient with pleural and lymph node tuberculosis diagnosed by pleural effusion characteristics and biopsy specimens, without mt identification, that further presented with knee-joint involvement. arthrocentesis allowed mt isolation and drug su ... | 2015 | 22421741 |
| evaluation of the nitrate reductase assay for the rapid detection of resistance to first-line medications in mycobacterium tuberculosis strains isolated from patients in a general hospital. | we compared the nitrate reductase assay with the proportion method, which is considered the gold standard, in 57 mycobacterium tuberculosis strains isolated from patients treated at the federal university of minas gerais hospital das clínicas, located in the city of belo horizonte, brazil. for rifampin and isoniazid, the sensitivity, specificity, and accuracy of the nitrate reductase assay were all 100%, whereas they were 100%, 88.9%, and 66.7%, respectively, for streptomycin and 98.0%, 100%, an ... | 2015 | 22576429 |
| [comparison of the activity of isoniazid and isoniazid methanesulfonate. ii. in guinea pigs experimentally infected with mycobacterium tuberculosis (h37 rv)]. | 2015 | 24543417 | |
| [comparative studies on isoniazid and calcium methanesulfonate of isonicotinic acid hydrazide (erbazid). i. experiments in rats infected with massive doses of mycobacterium tuberculosis]. | 2015 | 24543194 | |
| [determination of isoniazid sensitivity in mycobacterium tuberculosis]. | 2015 | 24542790 | |
| prevalence of multidrug-resistant tuberculosis cases among hiv-positive and hiv-negative patients eligible for retreatment regimen in togo using genexpert mtb/rif. | drug-resistant tuberculosis (tb) is emerging as an important health problem in togo. from sputum of previously treated tb patients, multidrug-resistant (mdr) tb was diagnosed in 24% (10/42) patients via genexpert mtb/rif compared to 25% (6/24) patients via conventional drug susceptibility testing (bactec mgit 960 system). the agreement between these two methods to detect mdr-tb is excellent. however, genexpert mtb/rif offers the advantage of rapidly detecting mycobacterium tuberculosis complex i ... | 2015 | 28626586 |
| [etiology of bacterial infections and incidence of comorbidities in patients with tuberculosis, treated in mazovian treatment centre of tuberculosis and lung diseases during years 2012-2014]. | tuberculosis (tb) is an infectious disease caused by the mycobacterium tuberculosis. infection occurs mostly via inhalation, while the immune system is weakened. tb can take a pulmonary or extrapulmonary form. treatment involves an intensive, long-term antimycobacterial multidrug therapy. tb cases are recorded on the worldwide scale. the morbidity in poland varies territorially. | 2015 | 28501834 |
| vitamin d, serum 25(oh)d, ll-37 and polymorphisms in a canadian first nation population with endemic tuberculosis. | background canadian first nation populations have experienced endemic and epidemic tuberculosis (tb) for decades. vitamin d-mediated induction of the host defence peptide ll-37 is known to enhance control of pathogens such as mycobacterium tuberculosis. objective evaluate associations between serum levels of 25-hydroxy vitamin d (25(oh)d) and ll-37, in adult dene first nation participants (n = 34) and assess correlations with single nucleotide polymorphisms (snps) in the vitamin d receptor (vdr) ... | 2015 | 28417792 |
| worms and humans: a happy divorce? | chronic asymptomatic worm infection, often in combination with tuberculosis (tb), is common in low-income countries. indeed, a life without worm infestation, as is now the case in most high-income countries, is a recent condition for humankind. worms and mycobacterium tuberculosis give rise to different immune response patterns (th2 vs. th1 driven), and we have studied whether chronic worm infection affects the susceptibility to and control of tb in the low income country of ethiopia. our result ... | 2015 | 28392966 |
| drug resistance profiles of new- and previously treated patients with pulmonary tuberculosis in ibadan, nigeria. | information on tb drug resistance profiles and its' associated risk factors are scarce in nigeria despite the large burden of disease in the country. the study was designed to report drug resistance profiles of new- and previously treated patients with pulmonary tuberculosis (tb) in ibadan, nigeria. | 2016 | 28686829 |
| collision of three pandemics: the coexistence of cervical cancer, hiv infection, and prior tuberculosis in the sub-saharan country of botswana. | cervical cancer is the leading cause of cancer-related mortality in the developing world, where hiv and mycobacterium tuberculosis (tb) infection are also endemic. hiv infection is independently associated with increased morbidity and mortality among women with cervical cancer. tb is believed to increase the risk of malignancies and could cause chronic inflammation in the gynecologic tract. however, the relationship between cervical cancer and tb in settings hyperendemic for hiv is unknown. we f ... | 2016 | 28717682 |
| a genomics-led approach to deciphering the mechanism of thiotetronate antibiotic biosynthesis. | thiolactomycin (tlm) is a thiotetronate antibiotic that selectively targets bacterial fatty acid biosynthesis through inhibition of the β-ketoacyl-acyl carrier protein synthases (kasi/ii) that catalyse chain elongation on the type ii (dissociated) fatty acid synthase. it has proved effective in in vivo infection models of mycobacterium tuberculosis and continues to attract interest as a template for drug discovery. we have used a comparative genomics approach to uncover the (hitherto elusive) bi ... | 2016 | 28791099 |
| [tuberculosis in general practice in belgium : what's new?] | tuberculosis is still an important disease in belgium, mainly in big agglomerations. for this reason, knowledge of this entity by a general practitioner is very important. genetic tests able to identify the species of mycobacterium tuberculosis have been developed in recent years, but also identification of resistance to 1st and 2nd line treatment through genetic methods has been developed. the quick availability of results of this tests compared to the standard tests is a big advantage. treatme ... | 2016 | 28525233 |
| surface expression and genetic variants of toll-like receptor 2 in pulmonary tuberculosis patients. | innate immunity plays an important role in the host defense against mycobacterium tuberculosis (m. tuberculosis). toll-like receptor-2 (tlr2) is the main receptor and one of central components in initiation of innate immunity against m. tuberculosis. the study aimed to evaluate tlr2 surface expression on peripheral blood monocytes and study its association with variants in tlr2 597t/c single nucleotide polymorphisms (snps) in patients with pulmonary tuberculosis. fifty pulmonary tuberculosis pat ... | 2016 | 28502147 |