Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| chinese herbs (astragalus radix and ganoderma lucidum) enhance immune response of carp, cyprinus carpio, and protection against aeromonas hydrophila. | the effect of chinese herbs (astragalus radix and ganoderma lucidum) on immune response of carp was investigated. fish were fed diets containing astragalus (0.5%), ganoderma (0.5%) and combination of two herbs (astragalus 0.5% and ganoderma 0.5%) for 5 weeks. other groups of fish were vaccinated (i.p.) against aeromonas hydrophila/aeromonas salmonicida (shering plough, essex, u.k.) at the beginning of the experiment and fed the same diets as described above. control fish (negative control) and f ... | 2009 | 18817878 |
| classical crosses of common carp (cyprinus carpio l.) show co-segregation of antibody response with major histocompatibility class ii b genes. | in cyprinids, two paralogous groups of major histocompatibility (mh) class ii b genes, dab1 and dab3, have been reported but have not been studied in detail. in our study on mh association with immune responsiveness in common carp (cyprinus carpio l.) we have taken a long-term approach using divergent selection for antibody production. we report the co-segregation of cyca-dab1-like and cyca-dab3-like genes with antibody response, in backcrosses to high- and low-responsive parental carp lines. we ... | 2009 | 18817879 |
| allelic discrimination, three-dimensional analysis and gene expression of multiple transferrin alleles of common carp (cyprinus carpio l.). | we cloned and sequenced four different transferrin (tf) alleles (c, d, f and g) of european common carp (cyprinus carpio carpio l.) and studied allelic diversity with respect to differences in sequence, constitutive transcription and three-dimensional structure. most of the disulfide bonds were conserved between human and carp tf, and modeling confirmed the overall conservation of the three-dimensional structure of carp tf. while the iron-binding sites in the c-lobe of carp tf were completely co ... | 2009 | 18824109 |
| cloning, nucleotide sequence and phylogenetic analyses, and tissue-specific expression of the transferrin gene in cirrhinus mrigala infected with aeromonas hydrophila. | transferrin partial complementary dnas were cloned from the livers of five species in four genera of indian carps (indian major carp species: labeo rohita, catla catla and cirrhinus mrigala; medium carp: puntius sarana; minor carp: labeo bata) subsequent to polymerase chain reaction amplification with published heterologous primers or self-designed primers derived from conserved regions of transferrin cdna sequences. the partial transferrin cdnas of the five species of carps had sizes from 624 t ... | 2009 | 18718662 |
| effects of cyanobacterial extracts containing anatoxin-a and of pure anatoxin-a on early developmental stages of carp. | this study compares the effects of pure anatoxin-a and cyanobacterial extracts of an anatoxin-a producing strain on early stages of development of carp. carp eggs were exposed from 2:30 h to 4 days post-fertilization to different ecologically relevant concentrations of anatoxin-a, provided as pure toxin or contained in the cyanobacterial extracts. data on time to mortality, mortality rate, time to hatching, hatching rate, skeletal malformations rate, and larval standard length were registered un ... | 2009 | 18640721 |
| antibody response of two populations of common carp, cyprinus carpio l., exposed to koi herpesvirus. | common carp, cyprinus carpio l., exposed to koi herpesvirus (khv) may become persistently infected and populations containing such virus-infected individuals may transmit the virus to other fish when co-habited. detection of virus-infected fish in a population is thus critical to surveillance and control programmes for khv. a study was therefore designed to detect anti-khv serum antibodies, with an enzyme-linked immunosorbent assay, in common carp following experimental exposures to khv under va ... | 2009 | 19236553 |
| a hepatic protein modulates glucokinase activity in fish and avian liver: a comparative study. | glucokinase (gck) is a key enzyme involved in hepatic glucose metabolism as well as in glucose homeostasis regulation. in mammals, gck is regulated in vivo by a regulatory protein (gckr) through a nucleus-to-cytoplasm translocation enhanced by fructose 1-phosphate and counteracted by fructose 6-phosphate. there were no previous evidences in literature regarding the presence of gckr in livers of other vertebrates like fish and bird. accordingly, in the present study we assessed gckr presence in c ... | 2009 | 19247671 |
| simultaneous determination of microcystin contaminations in various vertebrates (fish, turtle, duck and water bird) from a large eutrophic chinese lake, lake taihu, with toxic microcystis blooms. | this is the first to conduct simultaneous determination of microcystin (mc) contaminations in multi-groups of vertebrates (fish, turtle, duck and water bird) from lake taihu with microcystis blooms. mcs (-rr, -yr, -lr) in microcystis scum was 328 microg g(-1) dw. mcs reached 235 microg g(-1) dw in intestinal contents of phytoplanktivorous silver carp, but never exceeded 0.1 microg g(-1) dw in intestinal contents of other animals. the highest mc content in liver of fish was in carassius auratus ( ... | 2009 | 19249079 |
| new insights into the evolution of ifns: zebrafish group ii ifns induce a rapid and transient expression of ifn-dependent genes and display powerful antiviral activities. | the ifns and their receptors have existed in early chordates for approximately 500 million years and represent the early elements in innate and adaptive immunity. both types i and ii ifns have been discovered in fish, and type i has recently been classified into two groups based on their primary protein sequences. however, the biological activities of fish ifns and their roles in infection are largely unknown. using the zebrafish and manageable bacterial (streptococcus iniae) and viral (spring v ... | 2009 | 19265122 |
| recombinant expression and affinity purification of snake venom gland parvalbumin in escherichia coli. | parvalbumins (pv) are small, acidic, water soluble and calcium-binding proteins generally present in muscular and nervous tissues. in the present study, we identified and characterized a cdna clone encoding pv, named aplpv, from a snake (agkistrodon piscivorus leucostoma) venom gland cdna library. aplpv belongs to ef-hand proteins with six alpha-helices constituting three ef-hand domains. the deduced amino acid sequence of aplpv is 91% and 68% identical to the previously characterized pvs of boa ... | 2009 | 19275943 |
| development and use of real-time reverse transcription-polymerase chain reaction assay to quantify cytochrome p4501a1 expression in american mink. | the distribution of natural populations of american mink is restricted to locales that are in proximity to aquatic ecosystems. because of the lipophilicity and persistence of polychlorinated biphenyls (pcbs) and reliance of mink on aquatic-based diets, mink at contaminated locales often bioacccumulate high levels of pcbs. in addition, in controlled laboratory studies, mink are highly sensitive at reproductive and developmental end points to the toxic effects of environmental pcb mixtures. it is ... | 2009 | 19277445 |
| two divergent leptin paralogues in zebrafish (danio rerio) that originate early in teleostean evolution. | we describe duplicate leptin genes in zebrafish (danio rerio) that share merely 24% amino acid identity with each other and only 18% with human leptin. we were also able to retrieve a second leptin gene in medaka (oryzias latipes). the presence of duplicate leptin genes in these two distantly related teleosts suggests that duplicate leptin genes are a common feature of teleostean fishes. despite low primary sequence conservation, we are confident in assigning orthology between mammalian and zebr ... | 2009 | 19293295 |
| involvement of gadd153 and cardiac ankyrin repeat protein in cardiac ischemia-reperfusion injury. | oxidative stress is critical for causing cardiac injuries during ischemia-reperfusion (ir), yet the molecular mechanism for this remains unclear. in the present study, we observe that hypoxia and reoxygenation, a component of ischemia, effectively induces apoptosis in the cardiac myocytes from neonatal rats and it concomitantly leads to induction of gadd153, an apoptosis-related gene. furthermore, ir injury of rat heart showed a gadd153 overexpression in the ischemic area where the tunel reactio ... | 2009 | 19299913 |
| differential binding of mammalian and salmon gnrhs with rat and carp pituitary receptors. | receptor binding of gnrh is connected with the stimulation of pituitary gonadotropic cells leading to both the release and biosynthesis of gonadotropins. the binding is connected with the conformational changes in the receptor which induce the specific intracellular signalisation. the study of fish gnrhs and their receptors may give us new knowledge of the complex interplay of different mechanisms involved in neuroendocrine regulation of reproduction. | 2009 | 19300396 |
| first description of a novel weissella species as an opportunistic pathogen for rainbow trout oncorhynchus mykiss (walbaum) in china. | six strains of gram-positive, catalase-negative, non-motile, irregular short rod-shaped weissella bacteria, with width and length of 0.5-0.6 and 1.2-2.7microm were isolated from diseased rainbow trout oncorhynchus mykiss (walbaum) in winter of 2007 at a commercial fishery in jingmen, hubei province, china. the diseased rainbow trout exhibited hemorrhage in eyes, anal region, intestine and abdomen wall, petechia of liver, some fish with hydrocele in stomach. six isolates had identical biochemical ... | 2009 | 19321272 |
| characterization of a pattern recognition molecule vitellogenin from carp (cyprinus carpio). | pattern recognition proteins function in innate immune responses by binding to molecules on the surface of invading pathogens and initiating host defense reactions. to explore the role of vitellogenin (vg) in fish innate immunity, we purified vg from carp by gel filtration combined with diethylaminoethyl (deae) chromatography. the purified vg was confirmed by maldi-tof mass spectrometry. antibacterial activity analysis showed that vg inhibited bacterial activity to escherichia coli and staphyloc ... | 2009 | 19327543 |
| resistance of common carp (cyprinus carpio l.) to cyprinid herpesvirus-3 is influenced by major histocompatibility (mh) class ii b gene polymorphism. | the role of mh class ii b (cyca-dab1-like) genes in resistance of common carp (cyprinus carpio l.) to cyprinid herpesvirus-3 (cyhv-3), also known as koi herpesvirus (khv) was analysed. the material consisted of 934 fish from six carp crosses. fish were challenged with cyhv-3 at an age of 7 and 10 months. during challenge experiments the peak of mortality caused by cyhv-3 was observed at days 8-12 p.i. and the overall cumulative mortality reached 79.9%. among six cyca-dab1-like genotypes, reveale ... | 2009 | 19328856 |
| the effects of ghrelin on the in vitro spontaneous and sgnrh-a stimulated luteinizing hormone (lh) release from the pituitary cells of common carp (cyprinus carpio l.). | in fish, like in mammals, ghrelin affects gonadotropin release acting at the level of the hypothalamus as well as directly on the pituitary gland. in the present study, enzymatically dispersed pituitary cells obtained from sexually mature male and female carp (cyprinus carpio l.) were incubated in the presence of human ghrelin at the concentration of 10(-7) or 10(-6) m, salmon gnrh analogue (des-gly(10), d-arg(6), trp(7), leu(8), pro(9))-lhrh (sgnrh-a) at the concentration of 10(-8) m or the com ... | 2009 | 19328862 |
| increased liver protein and mrna expression of natural killer cell-enhancing factor b (nkef-b) in ayu (plecoglossus altivelis) after aeromonas hydrophila infection. | natural killer cell-enhancing factor (nkef) may mediate cellular responses to proinflammatory molecules. the liver proteins of aeromonas hydrophila-infected ayu (plecoglossus altivelis) and healthy control fish were analyzed by 2de. a protein, which increased significantly in diseased fish, was identified as nkef-b by maldi-tof-ms. a full-length cdna clone of this proteinwas subsequently isolated. it contains 1092 bp with an open reading frame of 591 bp, coding for 197 amino acids with mw 21.9 k ... | 2009 | 19340957 |
| x-ray microanalysis (edxma) of cadmium-exposed eggs of bothriocephalus acheilognathi (cestoda: bothriocephalidea) and the influence of this heavy metal on coracidial hatching and activity. | over recent years it has been established that pollutants can have a significant impact on host-parasite systems in the aquatic environment, so much so that it has been proposed that parasite fauna may be a useful parameter to monitor water quality. surprisingly, with perhaps the exception of trematodes and bioaccumulation in adult acanthocephalans, detailed observations on the interaction between helminths, particularly cestodes, and pollutants such as heavy metals, are lacking. in this study, ... | 2009 | 19341741 |
| intron retention generates ankrd1 splice variants that are co-regulated with the main transcript in normal and failing myocardium. | the cardiac ankyrin repeat domain 1 protein (ankrd1, also known as carp) has been extensively characterized with regard to its proposed functions as a cardio-enriched transcriptional co-factor and stress-inducible myofibrillar protein. the present results show the occurrence of alternative splicing by intron retention events in the pig and human ankrd1 gene. in pig heart, ankrd1 is expressed as four alternatively spliced transcripts, three of which have non-excised introns: ankrd1-contained intr ... | 2009 | 19341785 |
| purification and characterization of alpha(1)-proteinase inhibitor and antithrombin iii: major serpins of rainbow trout (oncorhynchuss mykiss) and carp (cyprinus carpio) blood plasma. | the main serine proteinase inhibitors of rainbow trout (oncorhynchuss mykiss) and common carp (cyprinus carpio) blood plasma were isolated and purified. the investigated inhibitors, alpha(1)-proteinase inhibitor (alpha(1)-pi) and antithrombin iii (at iii), act by forming stable complexes with target proteinases. the association rate constants k (on) for the interaction of fish plasma inhibitors with several serine proteinases have been determined: k (on) for both carp and rainbow trout alpha(1)- ... | 2009 | 19343519 |
| vitellogenesis in the cestode atractolytocestus huronensis anthony, 1958 (caryophyllidea: lytocestidae). | vitellogenesis in the parthenogenetic caryophyllidean tapeworm atractolytocestus huronensis anthony, 1958, parasitizing the carp cyprinus carpio l., has been examined using light (lm) and transmission electron microscopy and cytochemical staining with periodic acid-thiosemicarbazide-silver proteinate for glycogen. it was found that formation of vitellocytes shares the same basic patterns in lower tapeworms with parthenogenetic and normal reproduction. each vitelline follicle of a. huronensis con ... | 2009 | 19352702 |
| upregulated expression of cardiac ankyrin repeat protein in human failing hearts due to arrhythmogenic right ventricular cardiomyopathy. | expression of cardiac ankyrin repeat protein (carp) is augmented in heart failure due to dilated or ischaemic cardiomyopathy. it is unclear whether carp is upregulated in heart failure due to arrhythmogenic right ventricular cardiomyopathy (arvc). in the present study, we investigated the expression pattern of carp and the correlation between carp and the well-known heart failure marker pro-atrial natriuretic peptide (proanp) in arvc failing hearts. | 2009 | 19359327 |
| [investigations of ectoparasite fauna of some fish species (cyprinus carpio linnaeus, 1758; cobitis simplicispinna hanko, 1924) from lake akşehir (konya)]. | in this study, the presence of metazoan ectoparasites on 7 cyprinus carpio and 90 cobitis simplicispinna from lake aksehir, turkey were investigated between july 2004 and june 2005. three parasite species were identified on the host fishes: gyrodactylus elegans nordmann, 1832 (42.9%, 293.6+/-482.0 parasite/fish) and dactylogyrus extensus mueller and van cleave, 1932 (85.7%, 9.8+/-6.8), were found on gills of c. carpio, and gyrodactylus cobitis bychowsky, 1933 (68.9%, 15.6+/-18.5) on gills and fi ... | 2009 | 19367558 |
| compositional characteristics of materials recovered from whole gutted silver carp (hypophthalmichthys molitrix) using isoelectric solubilization/precipitation. | isoelectric solubilization/precipitation (isp) at acidic and basic ph was applied to whole carp, yielding proteins, lipids, and insolubles. the objective was to characterize composition of recovered materials. crude protein was concentrated to 89-90% in proteins recovered at acidic ph and to 94-95% at basic ph. basic ph yielded proteins with more (p < 0.05) essential amino acids (eaas). eaa content in recovered proteins met fao/who/uno requirements. isp did not affect fatty acid (fa) composition ... | 2009 | 19368395 |
| the immunogenicity of viral haemorragic septicaemia rhabdovirus (vhsv) dna vaccines can depend on plasmid regulatory sequences. | a plasmid dna encoding the viral hemorrhagic septicaemia virus (vhsv)-g glycoprotein under the control of 5' sequences (enhancer/promoter sequence plus both non-coding 1st exon and 1st intron sequences) from carp beta-actin gene (pae6-g(vhsv)) was compared to the vaccine plasmid usually described the gene expression is regulated by the human cytomegalovirus (cmv) immediate-early promoter (pmcv1.4-g(vhsv)). we observed that these two plasmids produced a markedly different profile in the level and ... | 2009 | 19368775 |
| [influence of stress hormones (adrenalin and glucocorticoids) on the infestation rate of the carp cyprinus carpio l. by dactylogyrus sp. (monogenoidea)]. | influence of adrenaline, hydrocortisone, and prednisolone on the infestation rate of the carp cyprinus carpio by dactylogyrus sp. is investigated, according to the analysis of the abudance index of parasites. introduction of these hormones into experimental fish resulted in the increase of the abudance index of dactylogyrids. dependence of changes in the investigated parameters on the nature and character of the hormones' influence on the host immune system and metabolic processes is revealed. | 2009 | 19370984 |
| new insights into seafood allergy. | seafood plays an important role in human nutrition worldwide, sustained by international trade of a variety of new seafood products. increased production and consumption have resulted in more frequent reports of adverse reactions, highlighting the need for more specific diagnosis and treatment of seafood allergy. this review discusses recent literature in this field. | 2009 | 19398906 |
| differentiation between cyprinid herpesvirus type-3 lineages using duplex pcr. | to date, all the isolates of cyprinid herpesvirus type-3 (cyhv3) responsible for serious outbreaks in carps cyprinus carpio have been found to be very similar or identical on the basis of dna sequences of a few reference genes. however, two genetic lineages (u/i and j) are distinguished by full-length genome sequencing. two molecular markers presenting genetic variations were targeted for developing a duplex pcr assay able to distinguish cyhv3-u/i from cyhv3-j while avoiding dna sequencing. the ... | 2009 | 19428569 |
| new tools to study rna interference to fish viruses: fish cell lines permanently expressing sirnas targeting the viral polymerase of viral hemorrhagic septicemia virus. | previous studies have indicated that low transfection efficiency can be a major problem when gene inhibition by the use of small interfering rnas (sirnas) is attempted in fish cells. this may especially be true when targeting genes of viruses which are fast replicating and which can still infect cells that have not been transfected with the antiviral sirnas. to increase the amount of antiviral sirnas per cell a different strategy than transfection was taken here. thus, we describe carp epithelio ... | 2009 | 19428606 |
| immunostimulatory effects of natural human interferon-alpha (huifn-alpha) on carps cyprinus carpio l. | human interferon-alpha (huifn-alpha) is an important immunomodulatory substance used in the treatment and prevention of numerous infectious and immune-related diseases in animals. however, the immunostimulatory effects of huifn-alpha in fish remain to be investigated. in the current study, the immune responses of the carp species cyprinus carpio l. to treatment with huifn-alpha were analyzed via measurement of superoxide anion production, phagocytic activity and the expression of cytokine genes ... | 2009 | 19439367 |
| the antioxidant glutathione in the fish cell lines epc and bcf-2: response to model pro-oxidants as measured by three different fluorescent dyes. | reduced glutathione (gsh) protects cells against injury by oxidative stress and maintains a range of vital functions. in vitro cell cultures have been used as experimental models to study the role of gsh in chemical toxicity in mammals; however, this approach has been rarely used with fish cells to date. the present study aimed to evaluate sensitivity and specificity of three fluorescent dyes for measuring pro-oxidant-induced changes of gsh contents in fish cell lines: monochlorobimane (mbcl), 5 ... | 2009 | 19444932 |
| n-acetylcysteine provides dose-dependent protection against fenthion toxicity in the brain of cyprinus carpio l. | n-acetyl-l-cysteine, a low-molecular weight thiol compound, with two different doses was used to prevent fenthion, an organophosphorus insecticide and acaricide, related oxidative stress in the brain of a model organism, cyprinus carpio. fish were exposed to sub-lethal and nominal concentration of fenthion after intraperitoneal injection of 0.5 or 400 mg/kg nac. brain tissues were then dissected and homogenized to analyse gsh, gssg, tbars, and protein contents. enzymes that constitute the first ... | 2009 | 19444991 |
| differences in the antibody response and survival of genetically different varieties of common carp (cyprinus carpio l.) vaccinated with a commercial aeromonas salmonicida/a. hydrophila vaccine and challenged with a. hydrophila. | males of two strains of carp, wild duna (d), and inbred szarvas 22 (22), were selected for high and low stress response. two purebreds of d and 22, from randomly chosen parents and four crosses, 22 x 22-l (low stress response), 22 x 22-h (high stress response), 22 x d-l (low stress response) and 22 x d-h (high stress response) from selected stress response parents were produced and vaccinated with a commercial aeromonas salmonicida/aeromonas hydrophila vaccine and their circulating antibody resp ... | 2009 | 19449165 |
| determination of microcystin-lr and its metabolites in snail (bellamya aeruginosa), shrimp (macrobrachium nipponensis) and silver carp (hypophthalmichthys molitrix) from lake taihu, china. | this paper describes seasonal changes of microcystin-lr (mc-lr) and its glutathione (mc-lr-gsh) and cysteine conjugates (mc-lr-cys) in three aquatic animals--snail (bellamya aeruginosa), shrimp (macrobrachium nipponensis) and silver carp (hypophthalmichthys molitrix) collected from lake taihu, china. mc-lr, mc-lr-gsh, and mc-lr-cys were determined by liquid chromatography electrospray ionization mass spectrum (lc-esi-ms). the mean mc-lr concentrations in the hepatopancreas of snail and shrimp an ... | 2009 | 19473685 |
| in vivo exposure of susceptible and non-susceptible fish species to myxobolus cerebralis actinospores reveals non-specific invasion behaviour. | due to controversial previous results, it has been unclear whether myxobolus cerebralis (myxosporea, myxozoa) is able to specifically choose a salmonid host by selective attachment and penetration. using a novel in vivo tracking assay that employs fluorescence staining of actinospore sporoplasms, we demonstrate a lack of host specificity of m. cerebralis actinospores during their initial invasion reactions. fish were experimentally exposed to stained actinospores that could be detected as emitte ... | 2009 | 19476282 |
| involvement of pi3 kinase and map kinase in igf-i- and insulin-induced oocyte maturation in cyprinus carpio. | previously, we observed that in vitro germinal vesicle breakdown (gvbd) in cyprinus carpio oocytes was induced by recombinant human insulin-like growth factor-i (igf-i) and bovine insulin (b-insulin) and this induction was steroid-independent. to investigate further the early signal transduction components involved in this process, the possible role of phosphatidylinositol 3-kinase (pi3 kinase) during oocyte maturation was examined. igf-i- and b-insulin-induced oocyte maturation was significantl ... | 2009 | 19482057 |
| physiological responses to mercury in feral carp populations inhabiting the low ebro river (ne spain), a historically contaminated site. | the low ebro river course (northeast spain) is historically affected by mercury pollution due to a chlor-alkali plant operating at the town of flix for more than a century. river sediments analysed during the last 10 years showed high mercury levels in the river section starting just downstream the factory and spanning some 90km, down to the river delta. the possible environmental impact was studied by a combination of field and laboratory studies. mercury concentrations in liver, kidney and mus ... | 2009 | 19482362 |
| trematode centrocestus formosanus infection and distribution in ornamental fishes in mexico. | the aim of this study was to determine the ornamental fish species affected by the metacercariae of the digenean trematode centrocestus formosanus and its distribution in 48 fish farms in morelos, central mexico. the parasite was found to form various numbers of branchial cysts in 11 of the 25 species analyzed. goldfish carassius auratus was the most commonly affected species; 20 of 30 farms were positive for this parasite, the fish showing severe clinical signs and having a high mortality rate. ... | 2009 | 19485122 |
| distribution of the introduced cyprinid herpesvirus 3 in a wild population of common carp, cyprinus carpio l. | cyprinid herpesvirus 3 (cyhv-3), which causes a lethal disease in common carp, cyprinus carpio l., and koi, c. carpio koi, first occurred in lake biwa, japan in 2004. to elucidate distribution of cyhv-3 in a wild common carp population, we conducted a pcr survey of cyhv-3 among such fish in lake biwa in 2006. only 6% (1/18) of the common carp smaller than 300 mm were positive with pcr, whereas 31% (18/58) of fish larger than 300 mm were positive. to evaluate their past exposure to cyhv-3 infecti ... | 2009 | 19500210 |
| [polyclonal antibody preparation and expression pattern analysis of carp]. | the present study aims at preparation of carp antibody and analysis of carp expression pattern. | 2009 | 19500504 |
| epizootic haematopoietic necrosis virus--an assessment of the likelihood of introduction and establishment in england and wales. | epizootic haematopoietic necrosis virus (ehnv) is an iridovirus that affects perch (perca fluviatilis) and rainbow trout (oncorhynchus mykiss). it emerged in australia in the 1980s and has not been discovered elsewhere. it causes a high level of mortality in perch resulting in steep population declines. the main possible routes of introduction of the virus to england and wales are the importation of infected live fish or carcasses. however, no trade in live susceptible species is permitted under ... | 2009 | 19515438 |
| tapeworm khawia sinensis: review of the introduction and subsequent decline of a pathogen of carp, cyprinus carpio. | the asian tapeworm khawia sinensis hsü, 1935 (cestoda: caryophyllidea) is a large-sized (body length up to 11.5 cm) monozoic (unsegmented) parasite of common carp (cyprinus carpio l.) that may cause mortality of young fish (fry). since the 1960s, this cestode successfully colonized a large part of europe, including the british isles, north america and japan. however, a review of published records provides evidence that the tapeworm k. sinensis, invasive parasite of carp, has become less common d ... | 2009 | 19520514 |
| mutations in the ankrd1 gene encoding carp are responsible for human dilated cardiomyopathy. | dilated cardiomyopathy (dcm) is familial in approximately 30% of cases, and mutations have been identified in several genes. however, in a majority of familial cases, the responsible genes are still to be discovered. the ankrd1 gene is over-expressed in heart failure in human and animal models. the encoded protein carp interacts with partners such as myopalladin or titin, previously shown to be involved in dcm. we hypothesized that mutations in ankrd1 could be responsible for dcm. | 2009 | 19525294 |
| biochemical changes and sensory assessment on tissues of carp (cyprinus carpio, linnaeus 1758) during sale conditions. | in this study, some biochemical changes of carp (cyprinus carpio, linnaeus 1758) tissues were investigated. studies have been carried out on carp which have regional economical importance. storage temperature and time are the most important factors that affect the quality of fish during sales. it was observed that the temperature varied between 9 and 12 degrees c in sale conditions. in addition, we assumed the arrival time of the fish at the fish market to be 0 (zero) h. biochemical analyses [ma ... | 2009 | 19533401 |
| screening and characterization of potential probiotic lactic acid bacteria from cultured common carp intestine. | screening of potential probiotic lab for aquaculture from adult common carp intestine was performed seasonally. lactococcus lactis h2 and lactococcus raffinolactis h47, which show cholic acid resistance and strong antibacterial activity against fish pathogens, were selected from predominant lab in summer and winter respectively. enterococcus pseudoavium h50, with the strongest antimicrobial activity among the strains isolated through 1 year, was also selected. streptococcus iniae i1, with strong ... | 2009 | 19584561 |
| [studies on quantitative trait loci related to activity of lactate dehydrogenase in common carp (cyprinus carpio)]. | the reciprocal intergeneric hybrids between common wheat and roegneria kamoji were successfully obtained by means of embryo culture. morphology, chromosome pairing behavior at meiosis, fertility, and resistance to scab of the hybrid f1 and bc1 were studied. the results showed that the morphology of the reciprocal intergeneric hybrids f1 between r. kamoji and t. aestivum cv. chinese spring were intermediate type between the two parental species. the chromosome configuration at metaphase i (mi) of ... | 2009 | 19586894 |
| intracellular ankrd1 protein levels are regulated by 26s proteasome-mediated degradation. | the ankrd1/carp gene encodes a muscle-specific protein which has been implicated in transcriptional regulation and myofibrillar assembly. several features at both the mrna and protein levels define ankrd1 as a gene whose expression is tightly regulated, and deregulated expression of this protein has been recently associated to human congenital heart disease. it is therefore crucial to define the intracellular pathways that regulate the ankrd1 protein's steady-state levels. here, we show that ank ... | 2009 | 19589340 |
| phylogenetic analysis of spring viraemia of carp virus isolates from austria indicates the existence of at least two subgroups within genogroup id. | genetic relationships between 22 spring viraemia of carp virus (svcv) isolates from austria collected between 1994 and 2007 were determined based on the partial nucleotide sequence of the glycoprotein gene (g gene). phylogenetic analyses located all austrian isolates except one in genogroup id. one isolate collected in 2007 was placed within the svcv ia genogroup. more importantly, the study also revealed 3 distinct clusters within genogroup id, designated id1, id2 and id3. existence of subgroup ... | 2009 | 19593931 |
| comparison of the resistance of selected families of common carp, cyprinus carpio l., to koi herpesvirus: preliminary study. | 2009 | 19602097 | |
| characterization of the complete genome sequence of pike fry rhabdovirus. | the complete genome sequence of pike fry rhabdovirus (pfrv), consisting of 11,097 nucleotides, was determined. the genome contains five genes, encoding the nucleoprotein (n), phosphoprotein (p), matrix protein (m), glycoprotein (g), and rna-dependent rna polymerase (l) protein in the order 3'-n-p-m-g-l-5'. 3' leader- and 5' trailer-sequences in the pfrv genome show inverse complementarity. the pfrv proteins share the highest homology to the proteins of spring viremia of carp virus (svcv), rangin ... | 2009 | 19603256 |
| ankrd1, the gene encoding cardiac ankyrin repeat protein, is a novel dilated cardiomyopathy gene. | we evaluated ankyrin repeat domain 1 (ankrd1), the gene encoding cardiac ankyrin repeat protein (carp), as a novel candidate gene for dilated cardiomyopathy (dcm) through mutation analysis of a cohort of familial or idiopathic dcm patients, based on the hypothesis that inherited dysfunction of mechanical stretch-based signaling is present in a subset of dcm patients. | 2009 | 19608030 |
| cardiac ankyrin repeat protein gene (ankrd1) mutations in hypertrophic cardiomyopathy. | the purpose of this study was to explore a novel disease gene for hypertrophic cardiomyopathy (hcm) and to evaluate functional alterations caused by mutations. | 2009 | 19608031 |
| bioaccumulative characteristics of hexabromocyclododecanes in freshwater species from an electronic waste recycling area in china. | hexabromocyclododecanes (hbcds) are now emerging ubiquitous contaminants due to their wide usage, persistence and toxicities. to investigate the bioaccumulative characteristics of hbcds, sediments, winkle (littorina littorea), crucian carp (carassius carassius) and loach (misgurnus anguillicaudatus) were collected from two streams near an e-waste dismantling site in china, and hbcd exposure test was then conducted on chinese rare minnow. the concentration of hbcds was 14 ng g(-1) dry weight in s ... | 2009 | 19616822 |
| molecular analysis of spring viraemia of carp virus in china: a fatal aquatic viral disease that might spread in east asian. | spring viraemia of carp (svc) is a fatal viral disease for cyprinid fish, which is caused by spring viraemia of carp virus (svcv). to date, no svc outbreak has been reported in china. between 1998 and 2002, outbreaks of svc were reported in ornamental and wild fish in europe and america, imported from multiple sources including china. based on phylogenetic analysis, the viral strain isolated from america was shown to be originated from asia. these outbreaks not only resulted in huge economic los ... | 2009 | 19623265 |
| an evolutionary origin and selection process of goldfish. | many different physical characteristics of goldfish (carassius auratus auratus), such as celestial and telescopic eyes, fancy but uncontrollable shapes of tail fin, an unfittingly fat body, and loss of dorsal fins, provide us with a unique opportunity of studying artificial selection on phenotypic changes on the basis of molecular evolution. the aim of the present study is to elucidate the evolutionary origin and history of goldfish, taking into account the different characteristics of goldfish ... | 2009 | 19027055 |
| structure, organization and expression of common carp (cyprinus carpio l.) nkef-b gene. | natural killer (nk) cell enhancing factor (nkef) belongs to the newly defined peroxiredoxin (prx) family. its functions are to enhance nk cell cytotoxicity and to protect dna and proteins from oxidative damage. in this study, a partial cdna sequence of carp nkef-b was isolated from thymus cdna library. subsequently, the full-length cdna of carp nkef-b was obtained by means of 3' and 5' race, respectively. the full-length cdna of carp nkef-b was 1022 bp, consisting of a 73 bp 5'-terminal untransl ... | 2009 | 19032984 |
| differences between lipid content and composition of different parts of fillets from crossbred farmed carp (cyprinus carpio). | common carp (cyprinus carpio) is, globally, one of the most important farmed fish species. we have analyzed carp from a natural pond-production system in south bohemia. ongrowing fish had access to an abundant plankton population which was supplemented with cereals. fatty acid composition (fa) was investigated in three parts of fillets from four crosses of carp. the fa composition of the leanest part, the dorsal white muscle was similar to that of marine-farmed species; it contained a large prop ... | 2009 | 19043793 |
| functional characterization of the elr motif in piscine elr+cxc-like chemokine. | to elucidate the functional role of piscine incomplete elr motif, the recombinant cxc and its mutants (melr and mloop) were produced in escherichia coli m15 based on the predicted mature peptide coding sequence of the black sea bream cxc (bs cxc) chemokine. assays showed that the bs rcxc proteins displayed strong ability to induce fish blood neutrophils and head kidney (hk) macrophage migration in a dose-independent manner (10 to 200 ng), both in black sea bream and common carp. although the elr ... | 2009 | 19048342 |
| life cycle stages of heterophyid trematodes in vietnamese freshwater fishes traced by molecular and morphometric methods. | a survey of digenean zoonotic trematodes infecting snails and fishes in a north vietnamese freshwater fish culture system revealed shedding of three types of parapleurolophocercous cercariae from the snail host melanoides tuberculata and the presence of metacercariae within the genus haplorchis (h. pumilio and h. taichui) and procerovum sp. in tissues of cultured fishes (silver carp, indian carp and climbing perch). no morphological characters were able to link the different cercariae specifical ... | 2009 | 19056180 |
| the induction of nitric oxide response of carp macrophages by transferrin is influenced by the allelic diversity of the molecule. | the central role of transferrin (tf) as an iron transporting protein has been extended by observations that modified versions of tf also participate in the regulation of innate immunity. we report on the isolation of two carp tf proteins (alleles d and g) to purity using rivanol precipitation and ion-exchange chromatography, and describe the activation of head kidney-derived carp macrophages by cleaved tf. we demonstrate the superiority of the d-type over the g-type tf in inducing nitric oxide ( ... | 2009 | 18996204 |
| metal concentrations in water, sediment, and fish from sewage-fed aquaculture ponds of kolkata, india. | the concentrations of lead, cadmium, chromium, copper, and zinc were investigated in the sewage-fed pond water, sediment, and the various organs of labeo rohita, catla catla, cirrhinus mrigala, oreochromis mossambicus, and cyprinus carpio cultured in sewage-fed ponds, kolkata, india. among the metals, cadmium, lead, and zinc were detected in water and, except lead, were below the water quality guideline levels for the protection of freshwater aquatic life proposed by ceqg (canadian environmental ... | 2009 | 18998223 |
| structural abnormalities of common carp cyprinus carpio spermatozoa. | spermatozoa of common carp cyprinus carpio are typically consist of a primitive head without acrosome, a midpiece with several mitochondria, a centriolar complex (proximal and distal centriole), and one flagellum. during an evaluation of the motility of common carp spermatozoa, we found spermatozoa with more than one flagellum and/or "double head" in three different individuals. this may be related to abnormal spermatogenesis. ultrastructure and physiological parameters of spermatozoa were exami ... | 2009 | 18998229 |
| genetic differentiation among greek lake populations of carassius gibelio and cyprinus carpio carpio. | the genetic structure of the western greece lake populations of carassius gibelio and cyprinus carpio carpio populations was characterized by using a pcr-based rflp and sequencing analysis of mitochondrial rdna genes and regions (16s rdna, cytochrome b and d-loop). our analysis was able to detect: (a) two haplotypes in c. c. carpio populations and two haplotypes in c. gibelio populations (b) a high nucleotide divergence between the two species and (c) two genetically distinct c. gibelio populati ... | 2009 | 19005765 |
| detection of cyprinid herpesvirus 3 dna in river water during and after an outbreak. | the disease caused by cyprinid herpesvirus 3 (cyhv-3) brings catastrophic damages to cultivated carp and koi and to natural carp populations; however, the dynamics of the virus in environmental waters are unclear. in july 2007, cyhv-3 dna was detected in a dead common carp collected from the yura river in kyoto prefecture, japan, and this was followed by mass mortality. we collected water samples at eight sites along the yura river for 3 months immediately after confirmation of the disease outbr ... | 2009 | 19013729 |
| toxic effects of streptomyces griseus spores and exudate on gill pathology of freshwater fish. | many unexplained fish-kills in british waters are considered microbial in origin and a large proportion of field sites contains elevated concentrations of filamentous actinobacteria. the present study has shown that a strain of streptomyces griseus, isolated from field sites, elicits pathological changes to the gills of fish under laboratory conditions which mirror those found in situ. these changes include hyperplasia leading to fusion of the secondary lamellae and loss of microridging on the f ... | 2009 | 18755510 |
| genetic resistance of carp (cyprinus carpio l.) to trypanoplasma borreli: influence of transferrin polymorphisms. | in serum most of the iron molecules are bound to transferrin (tf), which is a highly polymorphic protein in fish. tf is an essential growth factor for mammalian trypanosomes. we performed a series of experiments with trypanoplasma borreli to detect putative correlations between different tf genotypes of common carp (cyprinus carpio l.) and susceptibility to this blood parasite. five genetically different, commercially exploited carp lines (israelian 'd', polish 'r2' and 'k', ukrainian 'ur', hung ... | 2009 | 18980781 |
| collateral circulation: past and present. | following an arterial occlusion outward remodeling of pre-existent inter-connecting arterioles occurs by proliferation of vascular smooth muscle and endothelial cells. this is initiated by deformation of the endothelial cells through increased pulsatile fluid shear stress (fss) caused by the steep pressure gradient between the high pre-occlusive and the very low post-occlusive pressure regions that are interconnected by collateral vessels. shear stress leads to the activation and expression of a ... | 2009 | 19101749 |
| goat serum as an alternative to establish cell culture from indian major carp, cirrhinus mrigala. | serum from goat, calf, and chicken sources were evaluated in terms of attachment, growth, and proliferation of explants of indian major carp, cirrhinus mrigala. the attachment of explants viz. heart, liver, and kidney was directly proportional to the concentration of the serum. among these sera, the highest percentage of attachment, growth, and proliferation was recorded for 10% goat serum and 15% newborn calf serum without affecting their cell morphology. on contrary to these sera, chicken seru ... | 2009 | 19118441 |
| cloning and expression of carp acetylcholinesterase gene in pichia pastoris and characterization of the recombinant enzyme. | the gene encoding acetylcholinesterase (ache) was cloned from common carp muscle tissue. the full-length cdna was 2368 bp that contains a coding region of 1902 bp, corresponding to a protein of 634 amino acids. the deduced amino acid sequence showed a significant homology with those of ichthyic aches and several common features among them, including t peptide encoded by exon t in the c-terminus. three yeast expression vectors were constructed and introduced into the yeast pichia pastoris. the tr ... | 2009 | 19121395 |
| study of the comparative dynamics of the incorporation of tissue free-water tritium (tfwt) in bulrushes (typha latifolia) and carp (cyprinus carpio) in the almaraz nuclear power plant cooling reservoir. | the almaraz nuclear power plant (spain) uses the water of arrocampo reservoir for cooling, and consequently raises the radioactive levels of the aquatic ecosystem of this reservoir. from july 2002 to june 2005, monthly samples of surface water, bulrushes (typha latifolia) and carp (cyprinus carpio) were collected from this reservoir. they were analyzed to determine the temporal evolution of the levels of (3)h in surface water and of its transfer from the surface water to free-water in the tissue ... | 2009 | 19128862 |
| cardiac ankyrin repeat protein is a marker of skeletal muscle pathological remodelling. | in an attempt to identify potential therapeutic targets for the correction of muscle wasting, the gene expression of several pivotal proteins involved in protein metabolism was investigated in experimental atrophy induced by transient or definitive denervation, as well as in four animal models of muscular dystrophies (deficient for calpain 3, dysferlin, alpha-sarcoglycan and dystrophin, respectively). the results showed that: (a) the components of the ubiquitin-proteasome pathway are upregulated ... | 2009 | 19143834 |
| high-density spore production of a b. cereus aquaculture biological agent by nutrient supplementation. | previous studies have demonstrated the efficacy of our bacillus cereus isolate (nrrl 100132) in reducing concentrations of nitrogenous wastes and inhibiting growth of fish pathogens. in vivo efficacy and tolerance to a range of physiological conditions in systems used to rear cyprinus carpio make this isolate an excellent candidate for aquaculture applications. production cost is an important consideration in development of commercially relevant biological products, and this study examines the o ... | 2009 | 19148635 |
| effects of fatiguing jumping exercise on mrna expression of titin-complex proteins and calpains. | eccentric exercise induced by electrostimulation increases mrna expression of titin-complex proteins in rodent skeletal muscle. in this study, mrna expression of titin, muscle lim protein (mlp), cardiac ankyrin repeat protein (carp), ankyrin repeat domain protein 2 (ankrd2), diabetes-related ankyrin repeat protein (darp), and calcium-activated proteinases, calpains, were investigated in human skeletal muscle after fatiguing jumping exercise. fatiguing jumping exercise did not change mrna express ... | 2009 | 19150862 |
| the major portal of entry of koi herpesvirus in cyprinus carpio is the skin. | koi herpesvirus (khv), recently designated cyprinid herpesvirus 3, is the causative agent of a lethal disease in koi and common carp. in the present study, we investigated the portal of entry of khv in carp by using bioluminescence imaging. taking advantage of the recent cloning of the khv genome as a bacterial artificial chromosome (bac), we produced a recombinant plasmid encoding a firefly luciferase (luc) expression cassette inserted in the intergenic region between open reading frame (orf) 1 ... | 2009 | 19153228 |
| role of waterborne copper on survival, growth and feed intake of indian major carp, cirrhinus mrigala hamilton. | the effect of copper on survival, growth and feed intake of indian major carp, cirrhinus mrigala (hamilton) fry (0.92 +/- 0.28 g) was studied for 60 days. survival rates of the fish exposed to control (0.02), 0.10, 0.15 and 0.23 mg l(-1) copper were 100%, 83 +/- 3%, 58 +/- 6%, and 50 +/- 4%, respectively. average daily growth was significantly (p < 0.05) lower at 0.15 and 0.23 mg l(-1) of copper. there was almost no growth at 0.23 mg l(-1) of copper. feed intake rates reduced significantly (p < ... | 2009 | 19165406 |
| transfer, distribution and bioaccumulation of microcystins in the aquatic food web in lake taihu, china, with potential risks to human health. | in this paper, accumulation and distribution of microcystins (mcs) was examined monthly in six species of fish with different trophic levels in meiliang bay, lake taihu, china, from june to november 2005. microcystins were analyzed by liquid chromatography electrospray ionization mass spectrometry (lc-esi-ms). average recoveries of spiked fish samples were 67.7% for mc-rr, 85.3% for mc-yr, and 88.6% for mc-lr. the mcs (mc-rr+mc-yr+mc-lr) concentration in liver and gut content was highest in phyt ... | 2009 | 19185334 |
| cyanophycin-degrading bacteria in digestive tracts of mammals, birds and fish and consequences for possible applications of cyanophycin and its dipeptides in nutrition and therapy. | to determine the susceptibility of cyanophycin granule polypeptide (cgp) to degradation by several mammalian, avian and fish gut flora. | 2009 | 19191948 |
| spermatological characters of the caryophyllidean cestode khawia sinensis hsü, 1935, a carp parasite. | spermatological characters of the caryophyllidean tapeworm khawia sinensis (lytocestidae), a carp parasite, have been investigated for the first time. the process of the spermatozoon formation corresponds in basic pattern to that of khawia armeniaca (brunanská and poddubnaya, parasitol res 99: 449-454, 2006). the presence of dense material at the early stages of spermiogenesis, the intercentriolar body, flagellar rotation (free flagellum plus flagellar bud), and a complete proximodistal fusion h ... | 2009 | 19727822 |
| nitric oxide hinders antibody clearance from the surface of trypanoplasma borreli and increases susceptibility to complement-mediated lysis. | trypanoplasma borreli is an extracellular blood parasite of carp belonging to the same order (kinetoplastida) as african trypanosomes. these mammalian parasites have developed different strategies to evade the host immune system including antigenic variation, immunosuppression and clearance of surface-bound antibodies. the latter mechanism allows trypanosomes to use their swimming movement to cause surface-bound antibodies to 'sail' and accumulate at the posterior end of the parasite, to be inte ... | 2009 | 19735943 |
| influence of cu2+-loaded silicate on the growth performance and microflora of crucian carp carassius auratus. | we investigated the effects of cu2+-loaded silicate (cls) on the growth performance, microflora of skin, gill and intestine, and intestinal morphology of crucian carp carassius auratus. a total of 225 native wild crucian carp, with an average initial body weight of 20 g, were randomly divided into 5 treatment groups using 3 replicate tanks of 15 fish per group. the dietary treatments were (1) basal diet, (2) basal diet + cuso4, (3) basal diet + silicate, (4) basal diet + 0.5% cls and (5) basal d ... | 2009 | 19750812 |
| a novel c1q family member with fucose-binding activity from surfperch, neoditrema ransonnetii (perciformes, embiotocidae). | the c1q family is a growing group of proteins with a globular c1q domain in the c-terminal region. we purified a new member of this family with l-fucose-binding activity from the plasma of surfperch, neoditrema ransonnetii through l-fucose-affinity chromatography and anion-exchange chromatography. n-terminal amino acid sequencing followed by cdna sequencing revealed that the protein was composed of 212 amino acids including a signal peptide of 20 amino acids. the gene expression analysis by rt-p ... | 2009 | 19772923 |
| sequence information, ontogeny and tissue-specific expression of complement component c3 in indian major carp, labeo rohita (hamilton). | the complement system is one of the first line of immune defence mechanisms as well as a modifier of acquired immunity. c3 is the central complement component primarily synthesized in liver. the local synthesis of c3 in tissues other than liver may play an important role in local inflammatory processes. the present study aims at looking into ontogeny of c3 in labeo rohita and its tissue-specific expression that is yet to be explored for indian carps. unfertilised eggs, and eggs after 0, 1, 3, 6, ... | 2009 | 19775074 |
| investigation of van gogh-like 2 mrna regulation and localisation in response to nociception in the brain of adult common carp (cyprinus carpio). | the van gogh-like 2 (vangl2) gene is typically associated with planar cell polarity pathways, which is essential for correct orientation of epithelial cells during development. the encoded protein of this gene is a transmembrane protein and is highly conserved through evolution. van gogh-like 2 was selected for further study on the basis of consistent regulation after a nociceptive stimulus in adult common carp and rainbow trout in a microarray study. an in situ hybridisation was conducted in th ... | 2009 | 19781599 |
| receptor-mediated and lectin-like activities of carp (cyprinus carpio) tnf-alpha. | functional characterization of tnf-alpha in species other than mammalian vertebrates is limited, and tnf-alpha has been studied in a limited number of fish species, primarily in vitro using recombinant proteins. studies on tnf-alpha from different fish species so far pointed to several inconsistencies, in particular with respect to some receptor-mediated activities of fish tnf-alpha, such as the ability to directly activate phagocytes. in the present study a comprehensive analysis of in vitro as ... | 2009 | 19786556 |
| identification of estrogen-like effects and biologically active compounds in river water using bioassays and chemical analysis. | the nackdong river is the longest river in south korea and passes through major cities that have several industrial complexes, including chemical, electric, and petrochemical complexes, and municipal characteristics such as apartment complexes. along the river, the gumi region has an electric industrial complex and an apartment complex that may be possible point sources of xenoestrogens such as phenolic compounds. to identify the causative chemicals for estrogenic activity in the river water of ... | 2009 | 19647290 |
| identification of some skeletal deformities in freshwater teleosts raised in egyptian aquaculture. | this study examines different forms of skeletal deformities detected in fish species collected from two egyptian aquaculture facilities during two harvest seasons in 2008. various patterns of skeletal deformities were observed in 19 of 959 fish collected. deformities were diagnosed using a number of techniques including clinical, radiographic, sonographic and histopathological evaluations. observed deformities included: lordosis and kyphosis in african catfish (clarius gariepenius); lateral proj ... | 2009 | 19647852 |
| pilot study on control of phytoplankton by zooplankton coupling with filter-feeding fish in surface water. | a pilot-scale facility was originally designed to control phytoplankton in algae-laden reservoir water characterized by summer cyanobacteria blooms (mainly microcystis flos-aquae). the system made good use of the different food habits of daphnia magna and silver carp. zooplankton (daphnia magna), filter-feeding fish (silver carp), and zooplankton (daphnia magna) were stocked in three separated tanks in sequence, respectively. thus, single-cell phytoplankton and some microcystis flos-aquae in sma ... | 2009 | 19657169 |
| determination of arsenic in foods by flow injection on-line sorption pre-concentration with hydride generation atomic fluorescence spectrometry. | a method was developed for the determination of total arsenic in foods using flow injection on-line sorption coupled with hydride generation atomic fluorescence spectrometry (hg-afs) using a cigarette filter as the sorbent material. after reducing as(v) to as(iii) by using l-cysteine, the determination of total arsenic was achieved through on-line formation and retention of the pyrrolidine dithiocarbamate arsenic complex (as(iii)-pdc) on the cigarette filter, which was packed in the pre-concentr ... | 2009 | 19680958 |
| experimental studies on the development of contracaecum rudolphii (nematoda: anisakidae) in copepod and fish paratenic hosts. | the larval development of the nematode contracaecum rudolphii (rudolphi, 1819), a common parasite of the proventriculus of cormorants, was experimentally studied. within the eggs cultivated in freshwater under laboratory temperatures of 20-22 degrees c, the developing larva undergoes two moults on days 4-5, attaining the third larval stage. most of the ensheathed third-stage larvae, 291-457 microm long, hatch spontaneously from egg shells on days 5-6. experiments have indicated that hatched ensh ... | 2009 | 19827362 |
| are all koi ulcer cases associated with infection by atypical aeromonas salmonicida? polymerase chain reaction assays of koi carp skin swabs submitted by hobbyists. | infection by atypical aeromonas salmonicida is regarded as the cause of ulcer disease (kud) in koi carp cyprinus carpio and goldfish carassius auratus. however, other causes--including parasites, viral infection, and fungi--have been proposed. in our diagnostic work, we often fail to isolate a. salmonicida even when clear clinical signs of kud are present. this failure may be because these fastidious and slow-growing bacteria are difficult to isolate in culture or because the bacteria are not ac ... | 2009 | 19873831 |
| effect of long-term exposure to simazine on histopathology, hematological, and biochemical parameters in cyprinus carpio. | we investigated if residues of simazine in the natural waters would cause histological, hematological, and biochemical alterations in carps from contaminated areas in badajoz (spain). some necrotic foci in kidney and liver, hepatitis, and hepatic steatosis were detected. no changes on measured hematological and biochemical parameters between fish from reference and contaminated ponds were observed. to assess if simazine exposure was the cause of these observations carps were exposed in the labor ... | 2009 | 18561303 |
| tracing the accumulation and effects of mercury uptake in the previtellogenic ovary of crucian carp, carassius auratus gibelio by autometallography and caspase-3 immunohistochemistry. | the aims of the present study were to apply the amg technique for localization of mercury at the light and electron microscopic level in the ovary of crucian carp after exposure to mercuric chloride and to find out if this heavy metal induces expression of caspase-3. depending on the stage of ovarian follicle development, two patterns of mercury accumulation have been found in previtellogenic ovary of crucian carp. the first mercury accumulation pattern has been found in the early previtellogeni ... | 2009 | 19085830 |
| oxidative stress and apoptosis of carassius auratus lymphocytes induced by nonplanar (pcb153) and coplanar (pcb169) polychlorinated biphenyl congeners in vitro. | among all the 209 kinds of polychlorinated biphenyls (pcbs) congeners, nonplanar and coplanar pcb congeners have different levels of toxicity on mammal cells such as neuronal cells, but little is known about their toxicity on fish cells although pcb congeners usually have high bioaccumulation abilities in the detected fish bodies. this study showed that 2,2',4,4',5,5'-hexacholorbiphenyl (pcb153, nonplanar congener) and 3,3',4,4',5,5'-hexacholorbiphenyl (pcb 169, coplanar congener) caused apoptos ... | 2009 | 19999978 |
| characterization of a novel tc1-like transposon from bream (cyprinidae, megalobrama) and its genetic variation in the polyploidy progeny of bream-red crucian carp crosses. | crossing the cyprinids diploid blunt snout bream megalobrama amblycephala (bsb) and carassius auratus red var. (rcc) generated sterile triploid (3nrb) and fertile tetraploid (4nrb) hybrid offspring. utilizing inverted terminal repeats (itrs) of transposon tdr1 from danio rerio as pcr primer, the results showed that evident change in the number of tc1-like transposons in 4nrb relative to bsb occurred, whereas such change did not arise in 3nrb compared to bsb. no tc1-like transposon was found in r ... | 2009 | 19826746 |
| intraperitoneal injection of extracted microcystins results in hypovolemia and hypotension in crucian carp (carassius auratus). | circulatory responses of crucian carp injected intraperitoneally with extracted microcystins (mcs) were studied at sublethal and lethal doses (150 and 600 microg mc kg(-1) body mass, respectively). mean arterial blood pressure (map), heart rate, hematocrit (hct), red blood cell (rbc) counts, and circulating blood volume (bv) were assayed at 0, 1, 3, 12, 24, and 48 h post-toxin administration. map decreased significantly in a dose-dependent manner over time. within the 48-h test period, the letha ... | 2009 | 19673078 |
| morphological and molecular phylogenetic analysis of two saprolegnia sp. (oomycetes) isolated from silver crucian carp and zebra fish. | two saprolegnia isolates, jy isolated from silver crucian carp (carassius auratus gibelio bloch) and bmy isolated from zebra fish (brachydanio rerio hamilton) came from infections occurring concurrently in different locations in china. to confirm whether the two isolates were from the same saprolegnia clone, comparative studies have been carried out based on their morphological, physiological and molecular characteristics. observations showed that morphologically (both asexual and sexual organs) ... | 2009 | 19640399 |
| glucose-6-phosphate isomerase is an endogenous inhibitor to myofibril-bound serine proteinase of crucian carp (carassius auratus). | glucose-6-phosphate isomerase (gpi) was purified to homogeneity from the skeletal muscle of crucian carp ( carassius auratus ) by ammonium sulfate fractionation, column chromatographies of q-sepharose, sp-sepharose, and superdex 200 with a yield of 8.0%, and purification folds of 468. the molecular mass of gpi was 120 kda as estimated by gel filtration, while on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page), two subunits (55 and 65 kda) were identified, suggesting that it ... | 2009 | 19472999 |
| in vitro generation of viral-antigen dependent cytotoxic t-cells from ginbuna crucian carp, carassius auratus langsdorfii. | little is known about antigen-specific t-cell responses to viruses in teleosts due to a lack of a suitable experimental system using inbred or clonal animals. in the present study we have successfully induced an in vitro generation of virus-specific cytotoxic t-cells (ctls) from isogeneic ginbuna crucian carp. responder cells (primarily lymphocytes) from crucian carp haematopoietic necrosis virus (chnv)-infected fish were capable of proliferating after stimulation in vitro with chnv-infected syn ... | 2009 | 19428040 |