Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| a unique biofilm proteome. | 2006 | 16933391 | |
| a comparison of three rapid bacterial detection methods under simulated real-life conditions. | bacterial screening of all produced platelet concentrates (pcs) is implemented in many countries to reduce the risk of transfusion-transmitted sepsis. this study compares three rapid bacterial detection methods by imitating real-life conditions. | 2006 | 16934073 |
| the variation of catalytic efficiency of bacillus cereus metallo-beta-lactamase with different active site metal ions. | the kinetics and mechanism of hydrolysis of the native zinc and metal substituted bacillus cereus (bcii) metallo-beta-lactamase have been investigated. the ph and metal ion dependence of k(cat) and k(cat)/k(m), determined under steady-state conditions, for the cobalt substituted bcii catalyzed hydrolysis of cefoxitin, cephaloridine, and cephalexin indicate that an enzyme residue of apparent pk(a) 6.3 +/- 0.1 is required in its deprotonated form for metal ion binding and catalysis. the k(cat)/k(m ... | 2006 | 16939217 |
| mitigation of olive mill wastewater toxicity. | the toxicity of olive mill wastewaters (omw) is commonly attributed to monomeric phenols. omw were treated in an aerated, stirred reactor containing agricultural soil, where the oxidative polymerization of phenols took place. in 24 h, omw monomeric phenols decreased by >90%. this resulted in a corresponding reduction in phytotoxicity, as measured by germination tests with tomato and english cress seeds, and in microbial toxicity, as measured by lag phase duration in bacillus cereus batch growth. ... | 2006 | 16939339 |
| synergistic bactericidal effect of carvacrol, cinnamaldehyde or thymol and refrigeration to inhibit bacillus cereus in carrot broth. | possible use of three different essential oil components as natural food preservatives was studied by examining their influence in the kinetics of growth from activated spores of four bacillus cereus strains in tyndallized carrot broth over the temperature range 5-16 degrees c. selected low concentrations of carvacrol, cinnamaldehyde, or thymol showed a clear antibacterial activity against b. cereus in the vegetable substrate. the addition of 2 microl cinnamaldehyde or 20 mg thymol to 100 ml of ... | 2006 | 16942988 |
| the use of fourier transform infrared spectroscopy to differentiate escherichia coli o157:h7 from other bacteria inoculated into apple juice. | fourier transform infrared spectroscopy (ft-ir) can discriminate escherichia coli o157:h7 atcc 35150 from other bacteria: e. coli atcc 25522, bacillus cereus atcc 10876, and listeria innocua atcc 51742 inoculated in to apple juice. spectra of bacterial suspensions (ca. 10(9) cfu/ml in 0.9% nacl) on anodisc (aluminum oxide) filters were tested. unique ft-ir vibrational combination bands from mid-ir active components of bacterial cells are present in the "fingerprint region" at wavenumbers between ... | 2006 | 16943000 |
| modeling the influence of electron beam irradiation on the heat resistance of bacillus cereus spores. | the effect of electron beam irradiation (ebi) on bacillus cereus spore heat resistance was investigated. irradiation with accelerated electrons had an important heat-sensitizing effect on distilled-water spore suspensions. after irradiation doses of 1.3, 3.1, or 5.7 kgy followed by heating at 90 degrees c, calculated d(90)-values for strains escuela politécnica superior de orihuela (epso)-41wr and epso-50ur were reduced more than 1.3, 2.4, and 4.6 times, respectively. plots of calculated log d(t ... | 2006 | 16943026 |
| thermal inactivation of bacillus cereus and clostridium perfringens vegetative cells and spores in pork luncheon roll. | the aim of this study was to design a thermal treatment(s) for pork luncheon roll, which would destroy bacillus cereus and clostridium perfringens vegetative cells and spores. b. cereus and c. perfringens vegetative and spore cocktails were used to inoculate luncheon meat. samples were subjected to different temperatures and removal times. the decimal-reduction times (d-values) were calculated by linear regression analysis (d = -1/slope of a plot of log surviving cells versus time). the log(10) ... | 2006 | 16943086 |
| purification, crystallization and preliminary x-ray crystallographic analysis of chitinase from bacillus cereus nctu2. | chitinases (ec 3.2.1.14) are found in a broad range of organisms, including bacteria, fungi and higher plants, and play different roles depending on their origin. a chitinase from bacillus cereus nctu2 (chinctu2) capable of hydrolyzing chitin as a carbon and nitrogen nutrient has been identified as a member of the family 18 glycoside hydrolases. chinctu2 of molecular weight 36 kda has been crystallized using the hanging-drop vapour-diffusion method. according to the diffraction of chitinase crys ... | 2006 | 16946479 |
| microbial transformation of aniline derivatives: regioselective biotransformation and detoxification of 2-phenylenediamine by bacillus cereus strain pda-1. | a bacterial isolate, strain pda-1, grew well on basal medium supplemented with 2-phenylenediamine, sucrose, and ammonium nitrate and completely transformed 2-phenylenediamine. the isolate was identified as bacillus cereus. the product formed from 2-phenylenediamine was identified by ei-ms and nmr as 2-aminoacetanilide; whole cells converted 2-phenylenediamine to the product with a 76% molar yield. whole cells also showed a broad substrate specificity toward 20 of 26 tested arylamines with substi ... | 2006 | 16952832 |
| control of enterotoxin gene expression in bacillus cereus f4430/73 involves the redox-sensitive resde signal transduction system. | in contrast to bacillus subtilis, the role of the two-component regulatory system resde has not yet been investigated in the facultative anaerobe bacillus cereus. we examined the role of resde in the food-borne pathogen b. cereus f4430/73 by constructing resde and rese mutants. growth performances, glucose metabolism, and expression of hemolysin bl (hbl) and nonhemolytic enterotoxin (nhe) were analyzed in the three strains under distinct oxygenation and extracellular oxidoreduction potential (or ... | 2006 | 16952956 |
| enzyme deactivation due to metal-ion dissociation during turnover of the cobalt-beta-lactamase catalyzed hydrolysis of beta-lactams. | metallo-beta-lactamases are native zinc enzymes that catalyze the hydrolysis of beta-lactam antibiotics but are also able to function with cobalt (ii) and require one or two metal ions for catalytic activity. the kinetics of the hydrolysis of benzylpenicillin catalyzed by cobalt substituted beta-lactamase from bacillus cereus (bcii) are biphasic. the dependence of enzyme activity on ph and metal-ion concentration indicates that only the di-cobalt enzyme is catalytically active. a mono-cobalt enz ... | 2006 | 16953588 |
| characterization of bacillus cereus isolates associated with fatal pneumonias: strains are closely related to bacillus anthracis and harbor b. anthracis virulence genes. | bacillus cereus is ubiquitous in nature, and while most isolates appear to be harmless, some are associated with food-borne illnesses, periodontal diseases, and other more serious infections. in one such infection, b. cereus g9241 was identified as the causative agent of a severe pneumonia in a louisiana welder in 1994. this isolate was found to harbor most of the b. anthracis virulence plasmid pxo1 (13). here we report the characterization of two clinical and one environmental b. cereus isolate ... | 2006 | 16954272 |
| capsule production in bacillus cereus strains associated with severe pneumonia. | we identified three encapsulated bacillus cereus strains, isolated from patients with severe pneumonia, in a collection of b. cereus isolates associated with human illness. we found that the extent of capsule expression was influenced by culturing conditions. our findings highlight consequent clinical and laboratory diagnostic challenges posed by such isolates. | 2006 | 16954292 |
| quantification of the effects of salt stress and physiological state on thermotolerance of bacillus cereus atcc 10987 and atcc 14579. | the food-borne pathogen bacillus cereus can acquire enhanced thermal resistance through multiple mechanisms. two bacillus cereus strains, atcc 10987 and atcc 14579, were used to quantify the effects of salt stress and physiological state on thermotolerance. cultures were exposed to increasing concentrations of sodium chloride for 30 min, after which their thermotolerance was assessed at 50 degrees c. linear and nonlinear microbial survival models, which cover a wide range of known inactivation c ... | 2006 | 16957208 |
| proteomic profiling and identification of immunodominant spore antigens of bacillus anthracis, bacillus cereus, and bacillus thuringiensis. | differentially expressed and immunogenic spore proteins of the bacillus cereus group of bacteria, which includes bacillus anthracis, bacillus cereus, and bacillus thuringiensis, were identified. comparative proteomic profiling of their spore proteins distinguished the three species from each other as well as the virulent from the avirulent strains. a total of 458 proteins encoded by 232 open reading frames were identified by matrix-assisted laser desorption ionization-time-of-flight mass spectro ... | 2006 | 16957262 |
| effect of different premilking manual teat-cleaning methods on bacterial spores in milk. | different teat-cleaning methods were evaluated to determine their effect on the presence of spores from anaerobic bacterial spore-formers in the milk. artificial contamination was used to achieve uniform contamination of teats to reduce the number of cows and samples needed in the experiments and still obtain adequate power to detect differences among tested methods. teats were contaminated experimentally with a large amount of clostridium tyrobutyricum spores in a manure-water slurry. various t ... | 2006 | 16960062 |
| growth stimulatory effect of ochrobactrum intermedium and bacillus cereus on vigna radiata plants. | this study assessed the plant growth-promoting ability of the bacterial strains ochrobactrum intermedium (isolate crt-1) and bacillus cereus (isolate s-6). | 2006 | 16965380 |
| differential identification of bacillus anthracis from environmental bacillus species using microarray analysis. | to determine whether microarray analysis could be employed for the differential identification of a range of environmental bacillus sp. from four strains of bacillus anthracis. | 2006 | 16968287 |
| phenolics and antimicrobial activity of traditional stoned table olives 'alcaparra'. | in the present work, we report the determination of phenolic compounds in 'alcaparra' table olives by reversed-phase hplc/dad, and the evaluation of their extract in vitro activity against several microorganisms that may be causal agents of human intestinal and respiratory tract infections, namely gram positive (bacillus cereus, bacillus subtilis, and staphylococcus aureus), gram negative bacteria (pseudomonas aeruginosa, escherichia coli, and klebsiella pneumoniae) and fungi (candida albicans a ... | 2006 | 16971127 |
| identification of the critical role of tyr-194 in the catalytic activity of a novel n-acyl-homoserine lactonase from marine bacillus cereus strain y2. | enzymatic disruption of quorum-sensing (qs) pathways in pathogenic organisms is a promising anti-infection therapeutic strategy. ahl-lactonase, a potent tool for biocontrol, can hydrolyze qs signal molecule n-acyl-homoserine lactones (ahls) into inactive products, thereby blocking the qs systems. a marine bacterial isolate y2, identified as a bacillus cereus subsp., was found capable of inactivating ahls. the aiia gene encoding the ahl-degrading enzyme from bacterial strain y2 was cloned and exp ... | 2006 | 16972128 |
| relapsing bacillus cereus peritonitis during automated peritoneal dialysis. | 2006 | 16973523 | |
| identification of bacillus cereus internalin and other candidate virulence genes specifically induced during oral infection in insects. | bacillus cereus is an opportunistic bacterium frequently associated with food-borne infections causing gastroenteritis. we developed an in vivo expression technology (ivet), with an insect host, for identification of the b. cereus genes specifically expressed during infection. this ivet-based approach uses site-specific recombinase tnpi to identify transient promoter activation. we constructed a genomic library of b. cereus atcc14579 by cloning dna fragments upstream from tnpi. the library was s ... | 2006 | 16978259 |
| detection and phylogenic analysis of one anthrax virulence plasmid pxo1 conservative open reading frame ubiquitous presented within bacillus cereus group strains. | the presence of one of the anthrax virulence plasmid pxo1 conserved fragments was analyzed in 24 bacillus cereus and b. thuringiensis strains, including 6 b. thuringiensis subspecies, by polymerase chain reactions. twelve out of 24 strains showed pcr-positive for an orf101 homologous sequence. two pxo1-orf101-like fragments from a b. cereus b-4ac and a commercial b. thuringiensis kurstaki hd1 were cloned, sequenced and expressed in escherichia coli. toxicity assays revealed that the product enco ... | 2006 | 16978581 |
| plcr papr-independent expression of anthrolysin o by bacillus anthracis. | cholesterol-dependent cytolysins (cdcs) are secreted, pore-forming toxins that are associated with pathogenesis in a variety of gram-positive bacteria. bacillus anthracis produces anthrolysin o (alo), a cdc that is largely responsible for the hemolytic activity of culture supernates when the bacterium is cultured in appropriate conditions. b. cereus and b. thuringiensis, species closely related to b. anthracis, produce cdcs with significant amino acid sequence homology to alo. transcription of t ... | 2006 | 16980467 |
| exsy and coty are required for the correct assembly of the exosporium and spore coat of bacillus cereus. | the exosporium-defective phenotype of a transposon insertion mutant of bacillus cereus implicated exsy, a homologue of b. subtilis cysteine-rich spore coat proteins coty and cotz, in assembly of an intact exosporium. single and double mutants of b. cereus lacking exsy and its paralogue, coty, were constructed. the exsy mutant spores are not surrounded by an intact exosporium, though they often carry attached exosporium fragments. in contrast, the coty mutant spores have an intact exosporium, alt ... | 2006 | 16980471 |
| prevalence of antibodies reactive to pathogenic and nonpathogenic bacteria in preimmune serum of new zealand white rabbits. | polyclonal antibodies are typically produced in rabbits. the rabbit's health plays an important role in the quality of antibodies produced. therefore, recommendations have been made by organizations on which bacteria and how frequently to test rabbit colonies. since it is well known that rabbits may contain cross-reactive antibodies in their preimmune serum, it is common to test the rabbits for reactivity prior to immunization. here preimmune sera from 19 different rabbits were tested with elisa ... | 2006 | 16981648 |
| examination of the structural and functional versatility of glms ribozymes by using in vitro selection. | self-cleaving ribozymes associated with the glms genes of many gram-positive bacteria are activated by binding to glucosamine-6-phosphate (glcn6p). representatives of the glms ribozyme class function as metabolite-sensing riboswitches whose self-cleavage activities down-regulate the expression of glms enzymes that synthesizes glcn6p. as with other riboswitches, natural glms ribozyme isolates are highly specific for their target metabolite. other small molecules closely related to glcn6p, such as ... | 2006 | 16982640 |
| hydroxymalonyl-acyl carrier protein (acp) and aminomalonyl-acp are two additional type i polyketide synthase extender units. | combinatorial biosynthesis of type i polyketide synthases is a promising approach for the generation of new structural derivatives of polyketide-containing natural products. a target of this approach has been to change the extender units incorporated into a polyketide backbone to alter the structure and activity of the natural product. one limitation to these efforts is that only four extender units were known: malonyl-coa, methylmalonyl-coa, ethylmalonyl-coa, and methoxymalonyl-acyl carrier pro ... | 2006 | 16983083 |
| phylogenetic analysis of bacillus cereus isolates from severe systemic infections using multilocus sequence typing scheme. | bacillus cereus strains from cases of severe or lethal systemic infections, including respiratory symptoms cases, were analyzed using multilocus sequence typing scheme of b. cereus mlst database. the isolates were evenly distributed between the two main clades, and 60% of them had allele profiles new to the database. half of the collection's strains clustered in a lineage neighboring bacillus anthracis phylogenetic origin. strains from lethal cases with respiratory symptoms were allocated in bot ... | 2006 | 16985296 |
| characteristics of ligand recognition by a glms self-cleaving ribozyme. | 2006 | 16986193 | |
| evaluation of the hygienic quality and associated public health hazards of raw milk marketed by smallholder dairy producers in the dar es salaam region, tanzania. | a cross-sectional study was conducted to determine three parameters of the quality of the raw milk marketed by milk selling points (msps) in dar es salaam region. total bacterial count (tbc) was used as an indicator of the microbial quality of the milk; antimicrobial residues were determined; and the california mastitis test (cmt) was used to screen for milk somatic cells as an indication of the mastitis level in the cows that provided the milk. moreover, a water sample at each msp was taken for ... | 2006 | 16986766 |
| antimicrobial activity of extracts of chemical races of the lichen pseudevernia furfuracea and their physodic acid, chloroatranorin, atranorin, and olivetoric acid constituents. | the antimicrobial activity and the mic values of the ethanol, chloroform, diethyl ether, and acetone extracts of the chemical races of pseudevernia furfuracea (var. furfuracea and var. ceratea) and their physodic acid, chloroatranorin, atranorin, and olivetoric acid constituents have been investigated against some microorganisms. nearly all extracts of both chemical races showed antimicrobial activity against aeromonas hydrophila, bacillus cereus, bacillus subtilis, listeria monocytogenes, prote ... | 2006 | 16989308 |
| effects of chitosan and a low-molecular-weight chitosan on bacillus cereus and application in the preservation of cooked rice. | shrimp chitosan with 95% deacetylation and low-molecular-weight chitosan (lmwc) isolated from chitosan hydrolysate were investigated for their effects on the growth of bacillus cereus and for use in the preservation of cooked rice. four strains of bacillus cereus were used: standard strain bcrc 10603 and three isolates (nos. 1 through 3) from cooked rice. the antibacterial activity of chitosan against b. cereus was greatly decreased when the reaction ph was changed from 6.0 to 7.0, but lmwc acti ... | 2006 | 16995520 |
| structure-activity relationships of bacillus cereus and bacillus anthracis dihydrofolate reductase: toward the identification of new potent drug leads. | new and improved therapeutics are needed for bacillus anthracis, the etiological agent of anthrax. to date, antimicrobial agents have not been developed against the well-validated target dihydrofolate reductase (dhfr). in order to address whether dhfr inhibitors could have potential use as clinical agents against bacillus, 27 compounds were screened against this enzyme from bacillus cereus, which is identical to the enzyme from b. anthracis at the active site. several 2,4-diamino-5-deazapteridin ... | 2006 | 17005826 |
| comparative analysis of two-component signal transduction systems of bacillus cereus, bacillus thuringiensis and bacillus anthracis. | members of the bacillus cereus group are ubiquitously present in the environment and can adapt to a wide range of environmental fluctuations. in bacteria, these adaptive responses are generally mediated by two-component signal transduction systems (tcss), which consist of a histidine kinase (hk) and its cognate response regulator (rr). with the use of in silico techniques, a complete set of hks and rrs was recovered from eight completely sequenced b. cereus group genomes. by applying a bidirecti ... | 2006 | 17005984 |
| significant passive protective effect against anthrax by antibody to bacillus anthracis inactivated spores that lack two virulence plasmids. | the protective-antigen (pa)-based cell-free vaccine is the only vaccine licensed for use against bacillus anthracis infection in humans. although the pa shows strong immunogenicity, the capsule or spore-associated somatic antigens may be important as additional vaccine targets for full protection against anthrax. in this study, the protective effect of spore-associated antigens against b. anthracis infection was determined. rabbits were immunized with formalin-fixed spores of a non-toxigenic une ... | 2006 | 17005989 |
| enterotoxigenic bacillus spp. dna fingerprint revealed in naturally contaminated nonfat dry milk powder using rep-pcr. | dry milk powders and functional ingredients frequently contain high levels of viable bacterial spores, some of which may result in growth of toxigenic bacillus spp. in reconstituted and temperature-abused foods. samples from nonfat dry milk (nfdm), infant milk formula (imf), coffee creamer, lecithin, and cocoa powder were subjected to a short heat treatment followed by enrichment in tryptone phosphate glucose yeast extract (tpgy) broth at 32 degrees c for 12-25 hours to obtain cell densities of ... | 2006 | 17009291 |
| [non-species-specific effects of unacylated homoserine lactone and hexylresorcinol, low molecular weight autoregulators, on the growth and development of bacteria]. | we conducted a comparative study of the effects of alpha-amino-gamma-butyrolactone, the common structural element of extracellular microbial regulators of the homoserine lactone (hsl) group, and of 4-n-hexylresorcinol, an autoregulator of the alkylhydroxybenzene (ahb) group, on the growth and development of gram-positive and gram-negative bacteria. we revealed non-species-specific effects of hsl and ahb and characterized their concentration dependencies. the addition of 10(-5)-10(-3) m hsl or 10 ... | 2006 | 17025172 |
| metalloantibiotics: synthesis, characterization and in-vitro antibacterial studies on cobalt (ii), copper (ii), nickel (ii) and zinc (ii) complexes with cloxacillin. | the synthesis and characterization of cloxacillin (clox) complexes with divalent metal ions [co (ii), cu (ii), ni (ii) and zn (ii)] is described. the nature of bonding of the chelated cloxacillin and the structures of the metal complexes have been elucidated on the basis of their physical and spectroscopic data. in all the complexes, the cloxacillin acts as a uninegatively charged bidentate ligand with coordination involving the carboxylate-o and endocyclic-n of the beta-lactam ring. the new com ... | 2006 | 17059178 |
| molecular characterization of a dna fragment harboring the replicon of pbmb165 from bacillus thuringiensis subsp. tenebrionis. | bacillus thuringiensis belongs to the bacillus cereus sensu lato group of gram-positive and spore-forming bacteria. most isolates of b. thuringiensis can bear many endogenous plasmids, and the number and size of these plasmids can vary widely among strains or subspecies. as far as we know, the replicon of the plasmid pbmb165 is the first instance of a plasmid replicon being isolated from subsp. tenebrionis and characterized. | 2006 | 17059605 |
| table olives from portugal: phenolic compounds, antioxidant potential, and antimicrobial activity. | the phenolic compounds composition, antioxidant potential, and antimicrobial activity of different table olives from portugal, namely, natural black olives "galega", black ripe olive "negrinha de freixo", protected designation of origin (pdo) "azeitona de conserva negrinha de freixo" olives, and "azeitona de conserva de elvas e campo maior" designation of origin (do) olives, were investigated. the analysis of phenolic compounds was performed by reversed-phase hplc/dad, and seven compounds were i ... | 2006 | 17061816 |
| inhibition of bacillus anthracis and potential surrogate bacilli growth from spore inocula by nisin and other antimicrobial peptides. | the ability of nisin, synthetic temporin analogs, magainins, defensins, and cecropins to inhibit bacillus anthracis, bacillus cereus, bacillus thuringiensis, bacillus mycoides, and bacillus subtilis growth from spore inocula was determined using well diffusion assays. nisin, magainin ii amide, and defensins were inhibitory in screening against b. anthracis sterne or b. cereus atcc 7004, but only nisin inhibited virulent b. anthracis strains. the mics of nisin against the 10 bacillus strains exam ... | 2006 | 17066940 |
| antimicrobial emulsion (coating) based on biopolymer containing neem (melia azardichta) and turmeric (curcuma longa) extract for wound covering. | polymeric bio-adhesives emulsion which is biodegradable and non-toxic containing antimicrobial agents can play an important role in preventing infection in wound covering and coating for surgical implants. therefore a bioadhesive polymer was synthesized by semi-interpenetrating network process using blend of shellac, casein and polyvinyl alcohol and maleic anhydride (ma) as reactive compatibilizer. the synthesized polymer was mixed with neem and turmeric extract and homogenized using an emulsifi ... | 2006 | 17075168 |
| application of high-throughput technologies to a structural proteomics-type analysis of bacillus anthracis. | a collaborative project between two structural proteomics in europe (spine) partner laboratories, york and oxford, aimed at high-throughput (htp) structure determination of proteins from bacillus anthracis, the aetiological agent of anthrax and a biomedically important target, is described. based upon a target-selection strategy combining ;low-hanging fruit' and more challenging targets, this work has contributed to the body of knowledge of b. anthracis, established and developed htp cloning and ... | 2006 | 17001104 |
| role of swarming migration in the pathogenesis of bacillus endophthalmitis. | bacillus cereus causes one of the most rapidly blinding forms of bacterial endophthalmitis. migration of b. cereus throughout the eye during endophthalmitis is a unique aspect of this disease that may contribute to intraocular virulence. this study was conducted to analyze the contribution of swarming and intraocular migration to the pathogenesis of experimental endophthalmitis. | 2006 | 17003440 |
| inhibitory effects of spice essential oils on the growth of bacillus species. | a series of essential oils of 11 turkish plant spices [black thyme, cumin, fennel (sweet), laurel, marjoram, mint, oregano, pickling herb, sage, savory, and thyme], used in foods mainly for their flavor, aromas, and preservation, in herbal tea, in alternative medicines, and in natural therapies, were screened for antibacterial effects at 1:50, 1:100, 1:250, and 1:500 dilutions by the paper disc diffusion method against six bacillus species (bacillus amyloliquefaciens atcc 3842, bacillus brevis f ... | 2006 | 17004909 |
| the effect of lemon, orange and bergamot essential oils and their components on the survival of campylobacter jejuni, escherichia coli o157, listeria monocytogenes, bacillus cereus and staphylococcus aureus in vitro and in food systems. | to investigate the effectiveness of oils and vapours of lemon (citrus limon), sweet orange (citrus sinensis) and bergamot (citrus bergamia) and their components against a number of common foodborne pathogens. | 2006 | 17105553 |
| water-soluble inhibitor on microbiologically influenced corrosion in diesel pipeline. | the effect of water-soluble corrosion inhibitor on the growth of bacteria and its corrosion inhibition efficiency were investigated. corrosion inhibition efficiency was studied by rotating cage test and flow loop techniques. the nature of biodegradation of corrosion inhibitor was also analyzed by using fourier transform infrared spectroscopy (ft-ir), nuclear magnetic resonance spectroscopy (nmr) and gas chromatography and mass spectrometer (gc-ms). the bacterial isolates (serratia marcescens ace ... | 2006 | 17110090 |
| antimicrobial activities of tea catechins and theaflavins and tea extracts against bacillus cereus. | we evaluated the antimicrobial activities of seven green tea catechins and four black tea theaflavins, generally referred to as flavonoids, as well as the aqueous extracts (infusions) of 36 commercial black, green, oolong, white, and herbal teas against bacillus cereus (strain rm3190) incubated at 21 degrees c for 3, 15, 30, and 60 min. the results obtained demonstrate that (i) (-)-gallocatechin-3-gallate, (-)-epigallocatechin-3-gallate, (-)-catechin-3-gallate, (-)-epicatechin-3-gallate, theafla ... | 2006 | 16496576 |
| [a study of the safety of probiotic bacilli]. | the aim of the study was to evaluate the biological safety of a range of probiotic bacilli, ingredients of the commercial preparations biosporin, bactisubtil, and irilis; their biological effects were scrutinized in an animal experiment. the study demonstrated that the strains b. subtilis 3, 07, and b. licheniformis 31, 09, ingredients of biosporin and irilis, unlike b. cereus ip 5832, an ingredient of bactisubtil, were not toxic, toxigenic, or virulent, and did not impair the antitoxic hepatic ... | 2006 | 16496957 |
| bioactive natural and semisynthetic latrunculins. | marine-derived macrolides latrunculins a and b, of the red sea sponge negombata magnifica, are the first marine natural products that have been found to reversibly bind to actin monomers and to disrupt its organization. latrunculins are structurally related to many antimicrobial and antiangiogenic macrolides. several grams of latrunculin b (1), together with a new latrunculin named latrunculin t (2), were isolated from a recent collection of n. magnifica. semisynthetic modifications of 1, includ ... | 2006 | 16499319 |
| microbiological quality of randomly selected ready-to-eat foods sampled between 2003 and 2005 in wales, uk. | since 1995, the publicly funded ready-to-eat food sampling and examination activities in wales have been coordinated and structured, using a novel approach for the identification of samples and premises. the latest set of data from this surveillance system reports the results from 3391 ready-to-eat foods sampled between november 2003 and march 2005. during this seventeen-month period all samples were examined for aerobic colony count, escherichia coli, listeria spp., bacillus cereus, salmonella, ... | 2006 | 16503065 |
| emetic toxin-producing strains of bacillus cereus show distinct characteristics within the bacillus cereus group. | one hundred representative strains of bacillus cereus were selected from a total collection of 372 b. cereus strains using two typing methods (rapd and ft-ir) to investigate if emetic toxin-producing hazardous b. cereus strains possess characteristic growth and heat resistance profiles. the strains were classified into three groups: emetic toxin (cereulide)-producing strains (n=17), strains connected to diarrheal foodborne outbreaks (n=40) and food-environment strains (n=43), these latter not pr ... | 2006 | 16503068 |
| isolation of mercury resistant bacteria and influence of abiotic factors on bioavailability of mercury -- a case study in pulicat lake north of chennai, south east india. | pulicat lake sediments are often severely polluted with mercury compounds and other toxic heavy metals. several mercury-resistant bacteria were isolated and identified from the sediments and all the isolates exhibited broad spectrum resistance (both organic and inorganic mercuric compounds). mercury volatilization showed that four of the isolated bacillus cereus strains were able to reduce water soluble ionic form of mercury into volatile form via the well known enzymatic reduction. the effect o ... | 2006 | 16504251 |
| evaluation of a gmp training of milkers in dairy goat farms in são paulo, brazil. | a good manufacturing practices (gmp) training course was applied for goat milkers and evaluated using microbiological analysis in milk before and after training. milkers from three dairy goat farms located in são paulo, brazil, were submitted to a one-hour course on gmp and recommended guidelines for milking. samples of raw milk were collected before and one to two months after training, and analysed for aerobic mesophilic, psychrotrophic, coliform, staphylococcus aureus and salmonella spp count ... | 2006 | 16507483 |
| improving farm management by modeling the contamination of farm tank milk with butyric acid bacteria. | control of contamination of farm tank milk (ftm) with the spore-forming butyric acid bacteria (bab) is important to prevent the late-blowing defect in semi-hard cheeses. the risk of late blowing can be decreased via control of the contamination level of ftm with bab. a modeling approach was applied to identify an effective control strategy at the farm level. the simulation model developed was based on a translation of the contamination pathway into a chain of unit operations. using various simul ... | 2006 | 16507677 |
| a novel bacteriocin, thuricin 17, produced by plant growth promoting rhizobacteria strain bacillus thuringiensis neb17: isolation and classification. | the aim of this study was to identify and characterize a compound produced by the plant growth promoting bacterium, bacillus thuringiensis non-bradyrhizobium endophytic bacterium 17. | 2006 | 16478494 |
| screening for antimicrobial activity of ten medicinal plants used in colombian folkloric medicine: a possible alternative in the treatment of non-nosocomial infections. | the antimicrobial activity and minimal inhibitory concentration (mic) of the extracts of bidens pilosa l., bixa orellana l., cecropia peltata l., cinchona officinalis l., gliricidia sepium h.b. & k, jacaranda mimosifolia d.don, justicia secunda vahl., piper pulchrum c.dc, p. paniculata l. and spilanthes americana hieron were evaluated against five bacteria (staphylococcus aureus, streptococcus beta hemolític, bacillus cereus, pseudomonas aeruginosa, and escherichia coli), and one yeast (candida ... | 2006 | 16483385 |
| characteristics of the glms ribozyme suggest only structural roles for divalent metal ions. | the glms ribozyme is a riboswitch class that occurs in certain gram-positive bacteria, where it resides within mrnas encoding glucosamine 6-phosphate synthase. members of this self-cleaving ribozyme class rapidly catalyze rna transesterification upon binding glcn6p, and genetic evidence suggests that this cleavage event is important for down-regulating glms protein expression. in this report, we present a refined secondary structure model of the glms ribozyme and determine the importance of a co ... | 2006 | 16484375 |
| exchange of glutamine-217 to glutamate of clostridium limosum exoenzyme c3 turns the asparagine-specific adp-ribosyltransferase into an arginine-modifying enzyme. | c3-like adp-ribosyltransferaseses are produced by clostridium species, bacillus cereus, and various staphylococcus aureus strains. the exoenzymes modify the low-molecular-mass gtpases rhoa, b, and c. in structural studies of c3-like exoenzymes, an artt-motif (adp-ribosylating turn-turn motif) was identified that appears to be involved in substrate specificity and recognition (han, s., arvai, a. s., clancy, s. b., tainer, j. a. (2001) j. mol. biol. 305, 95-107). exchange of gln217, which is a key ... | 2006 | 16411778 |
| a probabilistic modeling approach in thermal inactivation: estimation of postprocess bacillus cereus spore prevalence and concentration. | the survival of spore-forming bacteria is linked to the safety and stability of refrigerated processed foods of extended durability (repfeds). a probabilistic modeling approach was used to assess the prevalence and concentration of bacillus cereus spores surviving heat treatment for a semiliquid chilled food product. this product received heat treatment to inactivate nonproteolytic clostridium botulinum during manufacture and was designed to be kept at refrigerator temperature postmanufacture. a ... | 2006 | 16416909 |
| fate and effect of ingested bacillus cereus spores and vegetative cells in the intestinal tract of human-flora-associated rats. | the fate and effect of bacillus cereus f4433/73r in the intestine of human-flora-associated rats was studied using bacteriological culturing techniques and pcr-denaturing gradient gel electrophoresis in combination with cell assays and immunoassays for detection of enterotoxins. in faecal samples from animals receiving vegetative cells, only few b. cereus cells were detected. spores survived the gastric barrier well, and were in some cases detected up to 2 weeks after ingestion. selective growin ... | 2006 | 16420599 |
| potential of selected infant food formulas for production of bacillus cereus emetic toxin, cereulide. | cereulide producing bacillus cereus was isolated from randomly chosen commercial infant foods. the cereulide production in infant food formulas was investigated. when the reconstituted foods were inoculated with >10(5) cfu ml(-1) of cereulide producing b. cereus, 2 to 200 microg of cereulide per 100 ml of food accumulated during 24 h of non-refrigerated storage. the amount of cereulide measured in the foods by the accurate chemical assay (lc-ms) matched with that found by sperm micro assay, prov ... | 2006 | 16423419 |
| evaluation of the enhanced bacterial detection system for screening of contaminated platelets. | the pall third-generation enhanced bacterial detection system (ebds) was recently approved for detection of bacterial contamination in leukoreduced platelets (plts). the method is based on the measurement of the oxygen content as a marker for bacteria. ebds incorporates major modifications including removal of the sample-set filter, modification of the culture medium, and incubation with agitation of the sample pouch. | 2006 | 16441598 |
| characterization of a mercury-reducing bacillus cereus strain isolated from the pulicat lake sediments, south east coast of india. | pulicat lake sediments are often severely polluted with the toxic heavy metal mercury. several mercury-resistant strains of bacillus species were isolated from the sediments and all the isolates exhibited broad spectrum resistance (resistance to both organic and inorganic mercuric compounds). plasmid curing assay showed that all the isolated bacillus strains carry chromosomally borne mercury resistance. polymerase chain reaction and southern hybridization analyses using mera and merb3 gene prime ... | 2006 | 16447070 |
| attachment of different soil bacteria to arbuscular mycorrhizal fungal extraradical hyphae is determined by hyphal vitality and fungal species. | attachment of certain bacteria to living arbuscular mycorrhizal fungal extraradical hyphae may be an important prerequisite for interactions between these microorganisms, with implications for nutrient supply and plant health. the attachment of five different strains of gfp-tagged soil bacteria (paenibacillus brasilensis pb177 (pnf8), bacillus cereus va1 (pnf8), pseudomonas fluorescens sbw25 :: gfp/lux, arthrobacter chlorophenolicus a6g, and paenibacillus peoriae bd62 (pnf8)) to vital and nonvit ... | 2006 | 16451176 |
| purification and sequencing of cerein 7b, a novel bacteriocin produced by bacillus cereus bc7. | cerein 7b is a new bacteriocin produced simultaneously with cerein 7a by bacillus cereus bc7 in liquid brain heart infusion cultures. both bacteriocins are not synergistic. the two peptides have been purified to homogeneity by hydrophobic interaction, cation exchange and reverse-phase liquid chromatography. they can be distinguished by their n-terminal amino acid sequences n-gly-trp-gly-asp-val-leu (7a) and n-gly-trp-trp-asn-ser-trp-gly-lys (7b). pre-cerein 7b is 74 amino acids long and contains ... | 2006 | 16451187 |
| inhibition of the growth of paenibacillus larvae, the causal agent of american foulbrood of honeybees, by selected strains of aerobic spore-forming bacteria isolated from apiarian sources. | the bacterium paenibacillus larvae, the causative agent of american foulbrood disease of honeybee larvae, occurs throughout the world and is found in many beekeeping areas of argentina. the potential as biocontrol agents of antagonic aerobic spore-forming bacteria isolated from honey samples and other apiarian sources were evaluated. each isolate was screened against one strain of paenibacillus larvae (atcc 9545) by using a perpendicular streak technique. ten randomly selected bacterial strains ... | 2006 | 16458322 |
| strategy for identification of bacillus cereus and bacillus thuringiensis strains closely related to bacillus anthracis. | bacillus cereus strains that are genetically closely related to b. anthracis can display anthrax-like virulence traits (a. r. hoffmaster et al., proc. natl. acad. sci. usa 101:8449-8454, 2004). hence, approaches that rapidly identify these "near neighbors" are of great interest for the study of b. anthracis virulence mechanisms, as well as to prevent the use of such strains for b. anthracis-based bioweapon development. here, a strategy is proposed for the identification of near neighbors of b. a ... | 2006 | 16461679 |
| multiple-locus sequence typing analysis of bacillus cereus and bacillus thuringiensis reveals separate clustering and a distinct population structure of psychrotrophic strains. | we used multilocus sequence typing (mlst) to characterize phylogenetic relationships for a collection of bacillus cereus group strains isolated from forest soil in the paris area during a mild winter. this collection contains multiple strains isolated from the same soil sample and strains isolated from samples from different sites. we characterized 115 strains of this collection and 19 other strains based on the sequences of the clpc, dinb, gdpd, panc, purf, and yhfl loci. the number of alleles ... | 2006 | 16461712 |
| identification of nif genes in n2-fixing bacterial strains isolated from rice fields along the yangtze river plain. | the aim of this research was to identify nifh and nifhdkye ' genes in twenty strains of n2-fixing heterotrophic bacteria isolated from rice fields in the yangtze river plain. southern hybridization of the total dna from each strain was performed with the klebsiella pneumoniae nifhdkye ' gene probe (6.2 kb eco ri fragment from psa30) and the azospirillum brasilense nifh gene probe (0.6 kb eco ri-hin diii fragment from phu8). we found that eco ri fragments of total dna from aeromonas hydrophila hy ... | 2006 | 16463319 |
| atomic structure of a na+- and k+-conducting channel. | ion selectivity is one of the basic properties that define an ion channel. most tetrameric cation channels, which include the k+, ca2+, na+ and cyclic nucleotide-gated channels, probably share a similar overall architecture in their ion-conduction pore, but the structural details that determine ion selection are different. although k+ channel selectivity has been well studied from a structural perspective, little is known about the structure of other cation channels. here we present crystal stru ... | 2006 | 16467789 |
| dioxiranes generated in situ from pyruvates and oxone as environmentally friendly oxidizing agents for disinfection. | dioxiranes generated in situ from pyruvates (alpha-keto esters) and oxone have been found to be environmentally friendly oxidizing agents for disinfection. these oxidizing agents were highly effective for destruction of various strains of bacteria, fungi, and bacterial endospores in a wide temperature range with exceptional stability. notably, by using an aqueous solution of methyl pyruvate (1a) and oxone/nahco3, complete destruction of bacteria such as staphylococcus aureus and fungus penicilli ... | 2006 | 16468412 |
| a new protein superfamily includes two novel 3-methyladenine dna glycosylases from bacillus cereus, alkc and alkd. | soil bacteria are heavily exposed to environmental methylating agents such as methylchloride and may have special requirements for repair of alkylation damage on dna. we have used functional complementation of an escherichia coli tag alka mutant to screen for 3-methyladenine dna glycosylase genes in genomic libraries of the soil bacterium bacillus cereus. three genes were recovered: alkc, alkd and alke. the amino acid sequence of alke is homologous to the e. coli alka sequence. alkc and alkd rep ... | 2006 | 16468998 |
| antiviral activities of purified compounds from youngia japonica (l.) dc (asteraceae, compositae). | the ethanol extract of a biannual medicinal herb, youngia japonica (commonly known as oriental hawk's beard) was reported previously to have potent antiviral activity against respiratory syncytial virus (rsv) cultured in hep-2 cells. three anti-microbial agents, namely 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, and luteolin-7-o-glucoside were subsequently purified and chemically characterized from the ethanol extract of youngia japonica. the two dicaffeoylquinic acids exhibited promin ... | 2006 | 16469463 |
| anaerobic cells of bacillus cereus f4430/73 respond to low oxidoreduction potential by metabolic readjustments and activation of enterotoxin expression. | in the present study, a food-borne pathogen strain of bacillus cereus (f4430/73) was anaerobically grown in controlled-batch conditions under low initial oxidoreduction potential (orp=-148 mv) using hydrogen gas as reducing agent. its physiological characteristics, including growth, glucose fermentation capacity and enterotoxin production, were compared with anaerobic conditions generated by nitrogen gas (orp=+ 45 mv). the results showed that low orp affected growth mainly during the early stage ... | 2006 | 16470372 |
| purification and cytotoxic properties of bacillus cereus hemolysin ii. | the hemolysin ii from bacillus cereus, hlyii, is a member of the beta-barrel pore-forming toxin family of secreted microbial proteins that includes the staphylococcus aureus alpha-toxin. compared with other proteins of the family, hemolysin ii has 90 extra amino acids at its c-terminus. to examine more closely the cytotoxic and pore-forming properties of the protein, we have cloned and expressed it in escherichia coli. we developed a purification procedure for the matured hlyii protein from both ... | 2006 | 16380268 |
| antilisterial activity of grape juice and grape extracts derived from vitis vinifera variety ribier. | grape juice and skin and seed extracts of vitis vinifera var. ribier black table grapes were found to be highly inhibitory towards listeria monocytogenes. this grape juice was also active against all other listeria species tested but not against bacillus cereus, salmonella menston, escherichia coli, staphylococcus aureus or yersinia enterocolitica. fractionation of the extracts showed that the antilisterial activity was strongest in the polymeric phenolic fractions. two different types of active ... | 2006 | 16386816 |
| antioxidant and biocidal activities of carum nigrum (seed) essential oil, oleoresin, and their selected components. | in the present study, chemical constituents of the essential oil and oleoresin of the seed from carum nigrum obtained by hydrodistillation and soxhlet extraction using acetone, respectively, have been studied by gc and gc-ms techniques. the major component was dillapiole (29.9%) followed by germacrene b (21.4%), beta-caryophyllene (7.8%), beta-selinene (7.1%), and nothoapiole (5.8%) along with many other components in minor amounts. seventeen components were identified in the oleoresin (table 2) ... | 2006 | 16390196 |
| characterization of germination receptors of bacillus cereus atcc 14579. | specific amino acids, purine ribonucleosides, or a combination of the two is required for efficient germination of endospores of bacillus cereus atcc 14579. a survey including 20 different amino acids showed that l-alanine, l-cysteine, l-threonine, and l-glutamine are capable of initiating the germination of endospores of b. cereus atcc 14579. in addition, the purine ribonucleosides inosine and adenosine can trigger germination of the spores. advanced annotation of the b. cereus atcc 14579 genom ... | 2006 | 16391023 |
| a bacillus thuringiensis s-layer protein involved in toxicity against epilachna varivestis (coleoptera: coccinellidae). | the use of bacillus thuringiensis as a biopesticide is a viable alternative for insect control since the insecticidal cry proteins produced by these bacteria are highly specific; harmless to humans, vertebrates, and plants; and completely biodegradable. in addition to cry proteins, b. thuringiensis produces a number of extracellular compounds, including s-layer proteins (slp), that contribute to virulence. the s layer is an ordered structure representing a proteinaceous paracrystalline array whi ... | 2006 | 16391064 |
| autoinducer 2 affects biofilm formation by bacillus cereus. | cell-free supernatants from growing bacillus cereus strain atcc 10987 induced luminescence in a photorhabdus luminescens deltaluxs mutant, indicating the production of functional autoinducer 2 (ai-2). the exogenous addition of in vitro synthesized ai-2 had an inhibitory effect on biofilm formation by b. cereus and promoted release of the cells from a preformed biofilm. | 2006 | 16391139 |
| combined plate microbial assay (cpma): a 6-plate-method for simultaneous first and second level screening of antibacterial residues in meat. | this paper proposes an improved high throughput microbial method for the simultaneous performance of first and second level screening for antibacterial residues in meat. it is based on growth inhibition of b. subtilis on agar medium ph 6, 7.2 and 8, of b. cereus on agar medium ph 5.9, of m. luteus on agar medium ph 8 and of e. coli on agar medium ph 7.2 (research or first level screening) and on the use of confirmatory solutions (pase, paba, mgso4) for the identification or second level screenin ... | 2006 | 16393810 |
| basics of flow cytometry-based sterility testing of platelet concentrates. | flow cytometry (facs) is a common technique in blood banking. it is used, for example, for the enumeration of residual white blood cells in plasma and in cellular blood products. it was investigated whether it can also be applied for sterility testing of buffy coat-derived platelet concentrates (pcs). | 2006 | 16398729 |
| bacillus cereus is common in the environment but emetic toxin producing isolates are rare. | to determine the incidence of emetic toxin producing bacillus cereus in soil, animal faeces and selected vegetable produce to compare the results with the previously reported high incidence in rice paddy fields. to examine whether the emetic toxin has antibiotic activity. | 2006 | 16405680 |
| probiotics for animal nutrition in the european union. regulation and safety assessment. | probiotics are alive micro-organisms, generally bacteria but also yeasts than, when ingested alive in sufficient amount, they have a positive effect on the health going beyond the nutritional ones commonly known. probiotics may operate through a nutritional and/or health or sanitary effect. micro-organisms used in animal feed in the eu are mainly bacterial strains of gram-positive bacteria belonging to the types bacillus, enterococcus, lactobacillus, pediococcus, streptococcus and strains of yea ... | 2006 | 16563585 |
| in vitro antibacterial, antifungal & cytotoxic activity of some isonicotinoylhydrazide schiff's bases and their cobalt (ii), copper (ii), nickel (ii) and zinc (ii) complexes. | isonicotinoylhydrazide schiff's bases formed by the reaction of substituted and unsubstituted furyl-2-carboxaldehyde and thiophene-2-carboxaldehyde with isoniazid and, their co (ii), cu (ii), ni (ii) and zn (ii) complexes have been synthesized, characterized and screened for their in vitro antibacterial activity against mycobacterium tuberculosis, escherichia coli, klebsiella pneumoniae, proteus mirabilis, pseudomonas aeruginosa, salmonella typhi, shigella dysenteriae, bacillus cereus, corynebac ... | 2006 | 16570512 |
| a novel ftsz-like protein is involved in replication of the anthrax toxin-encoding pxo1 plasmid in bacillus anthracis. | plasmid pxo1 encodes the tripartite anthrax toxin, which is the major virulence factor of bacillus anthracis. in spite of the important role of pxo1 in anthrax pathogenesis, very little is known about its replication and maintenance in b. anthracis. we cloned a 5-kb region of the pxo1 plasmid into an escherichia coli vector and showed that this plasmid can replicate when introduced into b. anthracis. mutational analysis showed that open reading frame 45 (repx) of pxo1 was required for the replic ... | 2006 | 16585744 |
| detailed genomic analysis of the wbeta and gamma phages infecting bacillus anthracis: implications for evolution of environmental fitness and antibiotic resistance. | phage-mediated lysis has been an essential laboratory tool for rapidly identifying bacillus anthracis for more than 40 years, relying on the gamma phage derivative of a bacillus cereus prophage called w. the complete genomic sequences of the temperate w phage, referred to as wbeta, and its lytic variant gamma were determined and found to encode 53 open reading frames each, spanning 40,864 bp and 37,373 bp, respectively. direct comparison of the genomes showed that gamma evolved through mutations ... | 2006 | 16585764 |
| levels of glycine betaine in growing cells and spores of bacillus species and lack of effect of glycine betaine on dormant spore resistance. | bacteria of various bacillus species are able to grow in media with very high osmotic strength in part due to the accumulation of low-molecular-weight osmolytes such as glycine betaine (gb). cells of bacillus species grown in rich and minimal media contained low levels of gb, but gb levels were 4- to 60-fold higher in cells grown in media with high salt. gb levels in bacillus subtilis cells grown in minimal medium were increased approximately 7-fold by gb in the medium and 60-fold by gb plus hig ... | 2006 | 16585779 |
| structural basis of the sphingomyelin phosphodiesterase activity in neutral sphingomyelinase from bacillus cereus. | sphingomyelinase (smase) from bacillus cereus (bc-smase) hydrolyzes sphingomyelin to phosphocholine and ceramide in a divalent metal ion-dependent manner. bc-smase is a homologue of mammalian neutral smase (nsmase) and mimics the actions of the endogenous mammalian nsmase in causing differentiation, development, aging, and apoptosis. thus bc-smase may be a good model for the poorly characterized mammalian nsmase. the metal ion activation of sphingomyelinase activity of bc-smase was in the order ... | 2006 | 16595670 |
| structure of phage protein bc1872 from bacillus cereus, a singleton with new fold. | 2006 | 16596646 | |
| the bacillus anthracis chromosome contains four conserved, excision-proficient, putative prophages. | bacillus anthracis is considered to be a recently emerged clone within the bacillus cereus sensu lato group. the b. anthracis genome sequence contains four putative lambdoid prophages. we undertook this study in order to understand whether the four prophages are unique to b. anthracis and whether they produce active phages. | 2006 | 16600039 |
| antimicrobial, wound healing and antioxidant activity of plagiochasma appendiculatum lehm. et lind. | plagiochasma appendiculatum (aytoniaceae) of the order marchantiales is widely used in the form of paste ethnomedicinally by gaddi tribe in kangra valley for treating skin diseases. in this context, antimicrobical potential of plagiochasma appendiculatum against a wide range of microorganisms was studied. to validate the ethnotherapeutic claims of the plant in skin diseases, wound healing activity was studied, besides antioxidant activity to understand the mechanism of wound healing activity. th ... | 2006 | 16600543 |
| bioactive principles in the bark of pilidiostigma tropicum. | the crude dichloromethane bark extract of pilidiostigma tropicum (myrtaceae) from north queensland, australia, shows antibacterial and cytotoxic activity. bioactivity-directed separation led to the isolation of rhodomyrtoxin b and ursolic acid-3-p-coumarate as the biologically active materials. the structures of these compounds were elucidated on the basis of spectral analysis. the intercalation interaction of rhodomyrtoxin b with dna was investigated using molecular mechanics and ab initio mole ... | 2006 | 16601968 |
| growth of escherichia coli, pichia pastoris and bacillus cereus in the presence of the ionic liquids [bmim][bf4] and [bmim][pf6] and organic solvents. | the influence of the two most commonly used ionic liquids (1-butyl-3-methyl imidazolium tetrafluoroborate, [bmim][bf(4)], 1-butyl-3-methyl imidazolium hexafluorophosphate, [bmim][pf(6)]) and three selected organic solvents (dimethylsulfoxide, ethanol, methanol) on the growth of escherichia coli, pichia pastoris and bacillus cereus was investigated. [bmim][bf(4)] was toxic at 1% (v/v) on all three microorganisms. the minimal inhibitory concentration (mic) of [bmim][bf(4)] on e. coli growth was be ... | 2006 | 16614927 |
| antimicrobial activity of saponins from medicago sp.: structure-activity relationship. | the antimicrobial activity of saponins from medicago sativa, m. arborea and m. arabica against a selection of medically important yeasts, gram-positive and -negative bacteria was investigated. structure-activity growth inhibitory effects of related prosapogenins and sapogenins are also described. increasing antibiotic activity was observed going from the saponin extracts to the sapogenin samples, suggesting that the sugar moiety is not important for the antimicrobial efficacy. activity was espec ... | 2006 | 16619355 |
| pathogenomic sequence analysis of bacillus cereus and bacillus thuringiensis isolates closely related to bacillus anthracis. | bacillus anthracis, bacillus cereus, and bacillus thuringiensis are closely related gram-positive, spore-forming bacteria of the b. cereus sensu lato group. while independently derived strains of b. anthracis reveal conspicuous sequence homogeneity, environmental isolates of b. cereus and b. thuringiensis exhibit extensive genetic diversity. here we report the sequencing and comparative analysis of the genomes of two members of the b. cereus group, b. thuringiensis 97-27 subsp. konkukian serotyp ... | 2006 | 16621833 |
| influence of type of food on the kinetics and overall production of bacillus cereus emetic toxin. | potato puree and penne pasta were inoculated with cereulide producing b. cereus 5964a and b. cereus ns117. static incubation at 28 degrees c proved these two foods to be a better substrate for higher cereulide production (4,080 ng/g in puree and 3,200 ng/g in penne were produced by b. cereus 5964a during 48 h of incubation) compared with boiled rice (2,000 ng/g). this difference occurred despite b. cereus counts of more than 10(8) cfu/g in all three products. aeration of cultures had a negative ... | 2006 | 16629028 |