Publications

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improvement in mental depression with decreased thyrotropin response after administration of thyrotropin-releasing hormone. 20144116150
bladder mucosa graft for construction of male urethra.a graft of bladder mucosa was used to construct the urethra in 35 males. most were complicated hypospadias cases. in all patients there was insufficient prepucial or penile shaft skin available to use for making a substitute urethra. the bladder mucosa worked well in every case. there were no fistulas. there was no case with stenosis of the anastomosis between the graft and the patient's own urethral tissue. the one possible disadvantage encountered in using bladder mucosa was its tendency to be ...20143958881
some observations of 24 cases of status epilepticus. 20144183468
skin prolyl hydroxylase in patients with obstructive jaundice. 20144138073
resolution of proteins in the kidney stone matrix using high-performance liquid chromatography.the organic matrix accounts for 2-3% of the total stone weight and has been considered to play a role in stone formation and growth. thus far, fractionation of the matrix proteins has been insufficient due to low resolution and reproducibility. in this report the matrix proteins of 22 stones were resolved by means of high-performance liquid chromatography. following pulverization, the organic matrix was obtained by dialysis against edta. the average content of nondialyzable extractable proteins ...20144076273
infectious jejunitis with stricture after gastrojejunostomy.although bacterial overgrowth has often been reported in postgastrectomy patients, inflammatory stricture near the surgical anastomosis is rare. we describe a 36-year-old woman with infectious jejunitis and subsequent jejunal stenosis after gastrojejunostomy.20143993634
[status of bone scintigraphy in evaluating the course of total hip joint endoprosthesis (tep)]. 20144070597
duplex carotid sonography: criteria for stenosis, accuracy, and pitfalls.both carotid bifurcations were examined in 353 patients over a 20-month interval using a combination of real-time and pulsed doppler ultrasound (duplex scanning). angiographic correlation was available in 72 cases. stenosis of the internal carotid was evaluated using a doppler input frequency of 5 mhz and a scan angle of 60 degrees. a peak frequency shift of less than 3.5 khz was found to be a sign of less than or equal to 30% stenosis; 3.5-4 khz with moderate turbulence suggested 31-50% stenosi ...20143880910
the politics of pollution. 20144161204
evaluation of two c.t. scanners for dual energy applications. 20144026712
[successful fibrinolytic treatment with urokinase six weeks after leg and pelvic vein thrombosis (author's transl)]. 20144448142
[3-year experience in the treatment with several arrhythmias with ritmos (ajmalin)]. 20144184833
[prevention of isoimmunization in the rh system with anti-d gamma globulin]. 20144187684
simplified method of staining cytology smears by the papanicolaou method. 20144166951
[multiple tumoral calcinosis. a recent case]. 20144279029
[multiple tumoral calcinosis. a recent case]. 20144279029
spectroscopic models related to coenzymes and base pairs. i. the basis for hypochromism in the ultraviolet spectra of model systems related to nicotinamide--adenine dinucleotide. 20144294243
spectroscopic models related to coenzymes and base pairs. i. the basis for hypochromism in the ultraviolet spectra of model systems related to nicotinamide--adenine dinucleotide. 20144294243
[application of factor analysis to the study of eeg structure]. 20144130613
[absenteeism of mid-level health personnel and the delivery of nursing care]. 20144085030
[cardiomyopathy. new findings]. 20144064098
a case of congenital lymphoblastic leukemia: clinical course and significance of ultrasonography.a case of congenital leukemia in which texture changes in ultrasonography were followed up is reported. the patient was a male infant hospitalized on day 22 after birth with multiple bluish skin nodules. complete remission was achieved easily, but he relapsed early and died from the disease after six months. because of l1 morphology the disease was classified as acute lymphoblastic leukemia, but the leukemic cells lacked the characteristics of lymphoid lineage on cytochemical, cytogenetic, immun ...20143903283
new techniques for the mass spectrometry of natural products. 20143896991
the gap activity of type iii effector yope triggers killing of yersinia in macrophages.the mammalian immune system has the ability to discriminate between pathogens and innocuous microbes by detecting conserved molecular patterns. in addition to conserved microbial patterns, the mammalian immune system may recognize distinct pathogen-induced processes through a mechanism which is poorly understood. previous studies have shown that a type iii secretion system (t3ss) in yersinia pseudotuberculosis leads to decreased survival of this bacterium in primary murine macrophages by unknown ...201425165815
a direct link between the global regulator phop and the csr regulon in y. pseudotuberculosis through the small regulatory rna csrc.in this study we investigated the influence of the global response regulator phop on the complex regulatory cascade controlling expression of early stage virulence genes of yersinia pseudotuberculosis via the virulence regulator rova. our analysis revealed the following novel features: (1) phop activates expression of the csrc rna in y. pseudotuberculosis, leading to activation of rova synthesis through the csrabc-rovm cascade, (2) activation of csrc transcription is direct and phop is shown to ...201424786463
retracing the evolutionary path that led to flea-borne transmission of yersinia pestis.yersinia pestis is an arthropod-borne bacterial pathogen that evolved recently from yersinia pseudotuberculosis, an enteric pathogen transmitted via the fecal-oral route. this radical ecological transition can be attributed to a few discrete genetic changes from a still-extant recent ancestor, thus providing a tractable case study in pathogen evolution and emergence. here, we determined the genetic and mechanistic basis of the evolutionary adaptation of y. pestis to flea-borne transmission. rema ...201424832452
fast and sensitive detection of enteropathogenic yersinia by immunoassays.yersinia enterocolitica and yersinia pseudotuberculosis, the two yersinia species that are enteropathogenic for humans, are distributed worldwide and frequently cause diarrhea in inhabitants of temperate and cold countries. y. enterocolitica is a major cause of foodborne disease resulting from consumption of contaminated pork meat and is further associated with substantial economic cost. however, investigation of enteropathogenic yersinia species is infrequently performed routinely in clinical l ...201425355759
genome-wide evaluation of the interplay between caenorhabditis elegans and yersinia pseudotuberculosis during in vivo biofilm formation.the formation of an incapacitating biofilm on caenorhabditis elegans by yersinia pseudotuberculosis represents a tractable model for investigating the genetic basis for host-pathogen interplay during the biofilm-mediated infection of a living surface. previously we established a role for quorum sensing (qs) and the master motility regulator, flhdc, in biofilm formation by y. pseudotuberculosis on c. elegans. to obtain further genome-wide insights, we used transcriptomic analysis to obtain compar ...201425312958
aerobic bacterial community of american cockroach periplaneta americana,a step toward finding suitable paratransgenesis candidates.cockroaches mechanically spread pathogenic agents, however, little is known about their gut microbiota. identification of midgut microbial community helps targeting novel biological control strategies such as paratransgenesis. here the bacterial microbiota of periplaneta americana midgut, were identified and evaluated for finding proper paratransgenesis candidate.201426114142
enzymatic synthesis of bioinformatically predicted microcin c-like compounds encoded by diverse bacteria.abstract the trojan horse escherichia coli antibiotic microcin c (mcc) consists of a heptapeptide attached to adenosine through a phosphoramidate linkage. mcc is synthesized by the mccb enzyme, which terminally adenylates the ribosomally synthesized heptapeptide precursor mcca. the peptide part is responsible for mcc uptake; it is degraded inside the cell to release a toxic nonhydrolyzable aspartyl-adenylate. bionformatic analysis reveals that diverse bacterial genomes encoding mccb homologues a ...201424803518
genome-wide analysis of small rnas expressed by yersinia pestis identifies a regulator of the yop-ysc type iii secretion system.small noncoding rna (srna) molecules are integral components of the regulatory machinery for many bacterial species and are known to posttranscriptionally regulate metabolic and stress-response pathways, quorum sensing, virulence factors, and more. the yop-ysc type iii secretion system (t3ss) is a critical virulence component for the pathogenic yersinia species, and the regulation of this system is tightly controlled at each step from transcription to translocation of effectors into host cells. ...201424532772
iscr is essential for yersinia pseudotuberculosis type iii secretion and virulence.type iii secretion systems (t3ss) are essential for virulence in dozens of pathogens, but are not required for growth outside the host. therefore, the t3ss of many bacterial species are under tight regulatory control. to increase our understanding of the molecular mechanisms behind t3ss regulation, we performed a transposon screen to identify genes important for t3ss function in the food-borne pathogen yersinia pseudotuberculosis. we identified two unique transposon insertions in yptb2860, a gen ...201424945271
kawasaki disease-specific molecules in the sera are linked to microbe-associated molecular patterns in the biofilms.kawasaki disease (kd) is a systemic vasculitis of unknown etiology. the innate immune system is involved in its pathophysiology at the acute phase. we have recently established a novel murine model of kd coronary arteritis by oral administration of a synthetic microbe-associated molecular pattern (mamp). on the hypothesis that specific mamps exist in kd sera, we have searched them to identify kd-specific molecules and to assess the pathogenesis.201425411968
in vitro evaluation of antimicrobial features of vasopressors.drugs administered as intravenous infusion may be contaminated during several stages of production or preparation. however studies focusing on antibacterial effects of vasopressor drugs are very rare. this study investigates the in vitro antimicrobial activity of the clinically used forms of vasopressors.201424794449
transcriptional frameshifting rescues citrobacter rodentium type vi secretion by the production of two length variants from the prematurely interrupted tssm gene.the type vi secretion system (t6ss) mediates toxin delivery into both eukaryotic and prokaryotic cells. it is composed of a cytoplasmic structure resembling the tail of contractile bacteriophages anchored to the cell envelope through a membrane complex composed of the tssl and tssm inner membrane proteins and of the tssj outer membrane lipoprotein. the c-terminal domain of tssm is required for its interaction with tssj, and for the function of the t6ss. in citrobacter rodentium, the tssm1 gene d ...201425474156
microwave-assisted preparation of azachalcones and their n-alkyl derivatives with antimicrobial activities.two new azachalcones were prepared by both claisen-schmidt condensation and a simple environmentally trendy microwave-assisted method. ten new n-alkyl (c6,8,10,12,14)-substituted azachalconium bromides (3a-e, 4a-e) were prepared from compounds 1 and 2 with corresponding alkyl halides. the antimicrobial activities of all the compounds were tested against enterococcus faecalis, yersinia pseudotuberculosis, pseudomonas aeruginosa, escherichia coli and staphylococcus aureus micro-organisms.201424571646
lcrv delivered via type iii secretion system of live attenuated yersinia pseudotuberculosis enhances immunogenicity against pneumonic plague.here, we constructed a yersinia pseudotuberculosis mutant strain with arabinose-dependent regulated and delayed shutoff of crp expression (arac p(bad) crp) and replacement of the msbb gene with the escherichia coli msbb gene to attenuate it. then, we inserted the asd mutation into this construction to form χ10057 [δasd-206 δmsbb868::p(msbb) msbb(ec) δp(crp21)::tt arac p(bad) crp] for use with a balanced-lethal asd-positive (asd(+)) plasmid to facilitate antigen synthesis. a hybrid protein compos ...201425114109
study of effect of substitution of the penultimate amino acid residue on expression, structure, and functional properties of yersinia pseudotuberculosis ompy porin.the purpose of the study was to compare the expression of two yersinia pseudotuberculosis proteins, wild-type porin ompy and the mutant porin ompy designated as ompy-q having the uncharged amino acid residue gln instead of positively charged arg at the penultimate position in the same heterologous host. according to the literature, a similar substitution (lys to gln) of the penultimate amino acid residue in neisseria meningitidis porin pora drastically improved the assembly of the protein in the ...201425108332
rabbit monoclonal antibodies directed at the t3ss effector protein yopm identify human pathogenic yersinia isolates.the yersinia outer protein m (yopm) is a type 3 secretion system (t3ss)-dependent effector protein of yersinia enterocolitica, yersinia pseudotuberculosis and yersinia pestis. although yopm is indispensable for full virulence, its molecular functions still remain largely elusive. recently, we could identify the recombinant yopm (ryopm) protein derived from the y. enterocolitica strain 8081 (jb580) as a cell-penetrating protein, which down-regulates the expression of various pro-inflammatory cyto ...201424636859
mutant ompf porins of yersinia pseudotuberculosis with deletions of external loops: structure-functional and immunochemical properties.recombinant mutant ompf porins from yersinia pseudotuberculosis outer membrane were obtained using site-directed mutagenesis. here we used four ompf mutants where single extracellular loops l1, l4, l6, and l8 were deleted one at a time. the proteins were expressed in escherichia coli at levels comparable to full-sized recombinant ompf porin and isolated from the inclusion bodies. purified trimers of the mutant porins were obtained after dialysis and consequent ion-exchange chromatography. change ...201424530904
bacteria induce prolonged pmn survival via a phosphatidylcholine-specific phospholipase c- and protein kinase c-dependent mechanism.polymorphonuclear leukocytes (pmns) are essential for the human innate immune defense, limiting expansion of invading microorganisms. pmn turnover is controlled by apoptosis, but the regulating signaling pathways remain elusive, largely due to inherent differences between mice and humans that undermine use of mouse models for understanding human pmn biology. here, we aim to elucidate signal transduction mediating survival of human peripheral blood pmns in response to bacteria, such as yersinia p ...201424498214
expression and targeting of secreted proteins from chlamydia trachomatis.chlamydia trachomatis is an obligate intracellular pathogen that replicates in a vacuole termed the inclusion. many of the interactions of chlamydiae with the host cell are dependent upon bacterial protein synthesis and presumably exposure of these proteins to the cytosol. because of the dearth of genetic tools for chlamydiae, previous studies examining secreted proteins required the use of heterologous bacterial systems. recent advances in genetic manipulation of chlamydia now allow for transfo ...201424443531
iatrogenic factors in periodontal disease. 20144514055
[on assessing the results of determining carbon monoxide in the air]. 20144180756
[problems of the myeloproliferative syndrome from the clinician's viewpoint]. 20144184584
retinal macroaneurysms. 20144056287
postmyelographic ct evaluation of multiple blocks due to metastases.a case of intraspinal metastatic carcinoma that presented on combined cervical and lumbar puncture metrizamide myelography as complete blocks at t2 and t12 is described. the extent of disease in the long segment of the spinal canal was inaccessible to conventional myelography. this case illustrates the importance of postmyelogram ct for accurate assessment of disease and for treatment planning.20144031182
tissue-fat pulmonary embolism occurring in a patient with a severe pelvic fracture.the fat embolism syndrome following trauma is associated with fat droplets in the pulmonary microvasculature. this is the first case report of a large adipose tissue embolus obstructing a pulmonary artery after blunt injury. this embolism occurred during the resuscitation of a patient with a laceration of an iliac vein and disruption of the adjacent fatty tissue secondary to a severe pelvic fracture. theories of the pathogenesis of the fat embolism syndrome and implications for early therapeutic ...20144086762
[paroxysmal phenomena in multiple sclerosis].the different types and common features of paroxysmal attacks are illustrated by three case reports. the main cause for these attacks is multiple sclerosis, but the mechanism is still unknown: ephaptic activation of demyelinated neurons is discussed. "anticonvulsive therapy" leads to cessation of the attacks.20143932890
silencing urease: a key evolutionary step that facilitated the adaptation of yersinia pestis to the flea-borne transmission route.the arthropod-borne transmission route of yersinia pestis, the bacterial agent of plague, is a recent evolutionary adaptation. yersinia pseudotuberculosis, the closely related food-and water-borne enteric species from which y. pestis diverged less than 6,400 y ago, exhibits significant oral toxicity to the flea vectors of plague, whereas y. pestis does not. in this study, we identify the yersinia urease enzyme as the responsible oral toxin. all y. pestis strains, including those phylogenetically ...201425453069
caenorhabditis elegans bacterial pathogen resistant bus-4 mutants produce altered mucins.caenorabditis elegans bus-4 glycosyltransferase mutants are resistant to infection by microbacterium nematophilum, yersinia pestis and yersinia pseudotuberculosis and have altered susceptibility to two leucobacter species verde1 and verde2. our objective in this study was to define the glycosylation changes leading to this phenotype to better understand how these changes lead to pathogen resistance. we performed maldi-tof ms, tandem ms and gc/ms experiments to reveal fine structural detail for t ...201425296196
the importance of the magnesium transporter mgtb for virulence of yersinia pseudotuberculosis and yersinia pestis.mg(2+) has been shown to be an important signal controlling gene regulation via the phopq two-component regulatory system for a range of gram-negative bacteria, including yersinia pestis and yersinia pseudotuberculosis. the magnesium ion transporter mgtb is part of the complex phopq regulon, being upregulated in response to low mg(2+). despite the presence of other mg(2+) transport systems in yersinia, inactivation of mgtb had a significant effect on the ability of the bacteria to scavenge this ...201425234474
yersinia pseudotuberculosis st42 (o:1) strain misidentified as yersinia pestis by mass spectrometry analysis.we report here the draft sequence of strain ceb14_0017, alias hiad_dup, recovered from a human patient and initially identified as yersinia pestis by mass spectrometry analysis. genotyping based on tandem repeat polymorphism assigned the strain to yersinia pseudotuberculosis sequence type 42 (st42). the total assembly length is 4,894,739 bp.201424926044
caspase-8 mediates caspase-1 processing and innate immune defense in response to bacterial blockade of nf-κb and mapk signaling.toll-like receptor signaling and subsequent activation of nf-κb- and mapk-dependent genes during infection play an important role in antimicrobial host defense. the yopj protein of pathogenic yersinia species inhibits nf-κb and mapk signaling, resulting in blockade of nf-κb-dependent cytokine production and target cell death. nevertheless, yersinia infection induces inflammatory responses in vivo. moreover, increasing the extent of yopj-dependent cytotoxicity induced by yersinia pestis and yersi ...201424799700
bioluminescent tracking of colonization and clearance dynamics of plasmid-deficient yersinia pestis strains in a mouse model of septicemic plague.yersinia pestis 201 contains 4 plasmids ppcp1, pmt1, pcd1 and pcry, but little is known about the effects of these plasmids on the dissemination of y. pestis. we developed a plasmid-based luxcdabe bioreporter in y. pestis 201, y. pestis 201-pcd1(+), y. pestis 201-pmt1(+), y. pestis 201-ppcp1(+), y. pestis 201-pcry(+), y. pestis 201-p(-) and yersinia pseudotuberculosis pa36060 strains, and investigated their dissemination by bioluminescence imaging during primary septicemic plague in a mouse mode ...201424333143
temperature-dependence of yadbc phenotypes in yersinia pestis.yadb and yadc are putative trimeric autotransporters present only in the plague bacterium yersinia pestis and its evolutionary predecessor, yersinia pseudotuberculosis. previously, yadbc was found to promote invasion of epithelioid cells by y. pestis grown at 37 °c. in this study, we found that yadbc also promotes uptake of 37 °c-grown y. pestis by mouse monocyte/macrophage cells. we tested whether yadbc might be required for lethality of the systemic stage of plague in which the bacteria would ...201424222617
presence of salmonella spp., yersinia enterocolitica, yersinia pseudotuberculosis and escherichia coli o157:h7 in wild boars.the european wild boar populations are growing and spreading to new areas, which might constitute a threat to public health, since wild boar can harbour pathogens with the potential to cause serious illness in humans. tonsils, ileocaecal lymph nodes and faecal samples were collected from 88 swedish wild boars and analysed for the presence of the zoonotic pathogens salmonella spp., yersinia enterocolitica, y. pseudotuberculosis and enterohaemorrhagic escherichia coli o157:h7 (ehec). a combination ...201424512817
coregulation of host-adapted metabolism and virulence by pathogenic yersiniae.deciphering the principles how pathogenic bacteria adapt their metabolism to a specific host microenvironment is critical for understanding bacterial pathogenesis. the enteric pathogenic yersinia species yersinia pseudotuberculosis and yersinia enterocolitica and the causative agent of plague, yersinia pestis, are able to survive in a large variety of environmental reservoirs (e.g., soil, plants, insects) as well as warm-blooded animals (e.g., rodents, pigs, humans) with a particular preference ...201425368845
development of real-time pcr assays for specific detection of hmsh, hmsf, hmsr, and irp2 located within the 102-kb pgm locus of yersinia pestis.virulent isolates of three pathogenic yersinia species (yersinia pestis, yersinia pseudotuberculosis, and yersinia enterocolitica) harbor a 102-kb chromosomal region which encodes elements critical for virulence. a 35-kb high pathogenicity island is contained in this region, is a known virulence determinant, contains irp1 and irp2 iron-regulating genes. an additional segment, the 68-kb high pathogenicity island, contains genetic elements responsible for conferring the y. pestis pigmentation phen ...201425261118
the first imported human case of yersinia pseudotuberculosis serotype o1 septicemia presents with acute appendicitis-like syndrome in taiwan.human nonplague yersiniosis occurs more commonly in temperate regions than in tropical or subtropical regions. in taiwan, which is located in a subtropical region of southeast asia, only environmental isolates and human infection of yersinia enterocolitica were reported, but a human case of y. pseudotuberculosis infection had not been identified. we report the first person with y. pseudotuberculosis serotype o1 septicemia who presented with acute appendicitis-like syndrome and who was probably c ...201425103079
high prevalence of pathogenic yersinia enterocolitica in pig cheeks.samples from pork cuts for minced meat and cheeks from processing plants and a slaughterhouse, and modified atmosphere (ma) packaged pork from retail were studied to estimate the prevalence of pathogenic, i.e. virulence plasmid bearing, yersinia enterocolitica and yersinia pseudotuberculosis in pork, as well as to quantify pathogenic y. enterocolitica in pork cuts. pathogenic (virf-positive) y. enterocolitica was isolated from 17 pig cheeks (23%) but not from any of the ma-packaged 54 retail por ...201424929882
colonization of cecum is important for development of persistent infection by yersinia pseudotuberculosis.yersiniosis is a human disease caused by the bacterium yersinia pseudotuberculosis or yersinia enterocolitica. the infection is usually resolved but can lead to postinfectious sequelae, including reactive arthritis and erythema nodosum. the commonly used yersinia mouse infection model mimics acute infection in humans to some extent but leads to systemic infection and eventual death. here, we analyzed sublethal infection doses of y. pseudotuberculosis in mice in real time using bioluminescent ima ...201424891107
canis lupus familiaris involved in the transmission of pathogenic yersinia spp. in china.to investigate canines carrying pathogens associated with human illness, we studied their roles in transmitting and maintaining pathogenic yersinia spp. we examined different ecological landscapes in china for the distribution of pathogenic yersinia spp. in canis lupus familiaris, the domestic dog. the highest number of pathogenic yersinia enterocolitica was shown from the tonsils (6.30%), followed by rectal swabs (3.63%) and feces (1.23%). strains isolated from plague free areas for c. lupus fa ...201424861841
relation between serology of meat juice and bacteriology of tonsils and feces for the detection of enteropathogenic yersinia spp. in pigs at slaughter.the association between positive serology and culture detection of yersinia spp. in individual pigs was determined. pieces of diaphragm from 370 pig carcasses were collected for serological analysis, and tonsils and feces of the same carcass were collected for bacteriological analysis. detection of anti-yersinia antibodies in meat juice samples was done using an indirect enzyme-linked immunosorbent assay (elisa) based on yops (yersinia outer proteins). tonsils and feces were tested for the prese ...201424823493
homology analysis of pathogenic yersinia species yersinia enterocolitica, yersinia pseudotuberculosis, and yersinia pestis based on multilocus sequence typing.we developed a multilocus sequence typing (mlst) scheme and used it to study the population structure and evolutionary relationships of three pathogenic yersinia species. mlst of these three yersinia species showed a complex of two clusters, one composed of yersinia pseudotuberculosis and yersinia pestis and the other composed of yersinia enterocolitica. within the first cluster, the predominant y. pestis sequence type 90 (st90) was linked to y. pseudotuberculosis st43 by one locus difference, a ...201424131695
spectroscopic studies on hasa from yersinia pseudotuberculosis.heme acquisition system a (hasa) is known as a hemophore in gram-negative pathogens. the ferric heme iron is coordinated by tyr-75 and his-32 in holo-hasa from pseudomonas aeruginosa (hasapa). in contrast, in holo-hasa from yersinia pseudotuberculosis (hasayp), our spectroscopic studies suggest that only tyr-75 coordinates to the ferric heme iron. the substitution of gln-32 with alanine in hasayp does not alter the spectroscopic properties, indicating that gln-32 is not an axial ligand for the h ...201424857805
a type iii secretion system inhibitor targets yopd while revealing differential regulation of secretion in calcium-blind mutants of yersinia pestis.numerous gram-negative pathogens rely upon type iii secretion (t3s) systems to cause disease. several small-molecule inhibitors of the type iii secretion systems have been identified; however, few targets of these inhibitors have been elucidated. here we report that 2,2'-thiobis-(4-methylphenol) (compound d), inhibits type iii secretion in yersinia pestis, yersinia pseudotuberculosis, and pseudomonas aeruginosa. yopd, a protein involved in the formation of the translocon and regulatory processes ...201424247143
[first case of lung abscess due to yersinia pseudotuberculosis in japan].a 63-year-old previously healthy man was admitted to our hospital with diarrhea that had lasted for about 4 weeks, high fever and dyspnea. chest computed tomography showed consolidation with a low-density area in the right middle lobe and small nodules with feeding vessels in the right upper lobe. on day 8, a cavity was observed in the consolidation, and the lymph nodes in the mediastinum became necrotic. yersinia pseudotuberculosis (serotype 4b) was cultured from blood, bronchial washing fluid, ...201425199381
the pyruvate-tricarboxylic acid cycle node: a focal point of virulence control in the enteric pathogen yersinia pseudotuberculosis.despite our increasing knowledge of the specific pathogenicity factors in bacteria, the contribution of metabolic processes to virulence is largely unknown. here, we elucidate a tight connection between pathogenicity and core metabolism in the enteric pathogen yersinia pseudotuberculosis by integrated transcriptome and [(13)c]fluxome analysis of the wild type and virulence-regulator mutants. during aerobic growth on glucose, y. pseudotuberculosis reveals an unusual flux distribution with a high ...201425164818
effect of thermolabile toxin from yersinia pseudotuberculosis on functions of innate immunity cells.the thermolabile toxin of yersinia pseudotuberculosis produces a selective dose-dependent stimulating effect on functional activity of innate immunity cells. prolonged apoptosis-inducing action of the toxin was associated with activation of enzymes of the oxygen-dependent system (ldh and myeloperoxidase) at the early terms of observation (up to 3 h). in turn, increased number of macrophages with apoptotic changes was noted at the early stages of contact with the thermolabile toxin (5 h), and its ...201425110089
[regulation of yersinia pseudotuberculosis major porin expression in response to antibiotic stress].the ompf porin gene expression in yersinia pseudotuberculosis in response to antibiotics of two different classes (kanamycin and nalidixic acid) was analyzed using quantitative pcr and a fluorescence reporter system. both antibiotics downregulated the expression of the ompf gene. the nalidixic acid significantly reduced ompf expression, while kanamycin, for which porins are considered to be an alternative transport route, only slightly reduced the ompf level.201425080814
influence of phop and intra-species variations on virulence of yersinia pseudotuberculosis during the natural oral infection route.the two-component regulatory system phop/phoq has been shown to (i) control expression of virulence-associated traits, (ii) confer survival and growth within macrophages and (iii) play a role in yersinia infections. however, the influence of phop on virulence varied greatly between different murine models of infection and its role in natural oral infections with frequently used representative isolates of y. pseudotuberculosis was unknown. to address this issue, we constructed an isogenic set of ...201425075520
role of vamp3 and vamp7 in the commitment of yersinia pseudotuberculosis to lc3-associated pathways involving single- or double-membrane vacuoles.yersinia pseudotuberculosis can replicate inside macrophages by hijacking autophagy and blocking autophagosome acidification. in bone marrow-derived macrophages, the bacteria are mainly observed inside double-membrane vacuoles positive for lc3, a hallmark of autophagy. here, we address the question of the membrane traffic during internalization of yersinia investigating the role of vesicle- associated membrane proteins (vamps). first, we show that as in epithelial cells, yersinia pseudotuberculo ...201425046114
effector cd8+ t cells are generated in response to an immunodominant epitope in type iii effector yope during primary yersinia pseudotuberculosis infection.yope is a virulence factor that is secreted into host cells infected by yersinia species. the yope c-terminal domain has gtpase-activating protein (gap) activity. the yope n-terminal domain contains an epitope that is an immunodominant cd8(+) t cell antigen during primary infection of c57bl/6 mice with yersinia pseudotuberculosis. the characteristics of the cd8(+) t cells generated in response to the epitope, which comprises yope amino acid residues 69 to 77 (yope(69-77)), and the features of yo ...201424799630
two cases of abdominal pain in children with mesenteric lymphadenitis due to yersinia pseudotuberculosis infection. 201424793203
a cis-encoded srna controls the expression of fabh2 in yersinia.ysrh is a novel cis-encoded srna located on the opposite strand to fabh2, which is essential for fatty acid biosynthesis in bacteria. in this study, ysrh-mediated regulation of fabh2 expression was investigated in yersinia pseudotuberculosis. constitutive and inducible over-expression of ysrh decreased the mrna level of fabh2, while expression of downstream fabd and fabg remained unaffected. polynucleotide phosphorylase (pnpase) also played an important role in this regulation process by mediati ...201424735725
atypical yersinia pseudotuberculosis serotype o:3 isolated from hunted wild boars in italy.atypical yersinia pseudotuberculosis serotype o:3 was isolated from rectal contents of two wild boars hunted in italy within a regional wildlife management program. no outbreak of yersiniosis was reported in this area in the same period and no lesions were found by the veterinarian at post-mortem inspection. nevertheless, after histological examination, granulomatous lesions were detected in submandibular lymph nodes of one of the two wild boars. microbiological and bio molecular characterizatio ...201424698132
loop-mediated isothermal amplification assays for detecting yersinia pseudotuberculosis in milk powders.yersinia pseudotuberculosis is a gram-negative foodborne pathogen that causes several diseases, such as enteritis, septicemia, and reactive arthritis. loop-mediated isothermal amplification (lamp) assay targeting the 16s-23s rdna internal transcribed spacer (its) region was developed to detect y. pseudotuberculosis in milk powder. the dna amplification could be completed in 1 h, and detected by produced white precipitate visible to naked eyes. the detection limit of lamp assay was 10(0) fg/react ...201424697660
genome sequence of yersinia similis y228t, a member of the yersinia pseudotuberculosis complex.we report here on the genome sequence of yersinia similis 228(t) isolated in germany. the genome has a size of 4.9 mb and a g+c content of 47% and is predicted to contain 4,135 coding sequences. annotation of the 60,687-bp extrachromosomal element predicted 67 coding sequences and a g+c content of 47.8%.201424675860
typing and clustering of yersinia pseudotuberculosis isolates by restriction fragment length polymorphism analysis using insertion sequences.yersinia pseudotuberculosis is an enteropathogen that has an animal reservoir and causes human infections, mostly in temperate and cold countries. most of the methods previously used to subdivide y. pseudotuberculosis were performed on small numbers of isolates from a specific geographical area. one aim of this study was to evaluate the typing efficiency of restriction fragment length polymorphism of insertion sequence hybridization patterns (is-rflp) compared to other typing methods, such as se ...201424671793
lcrq blocks the role of lcrf in regulating the ysc-yop type iii secretion genes in yersinia pseudotuberculosis.pathogenic yersinia species employ the ysc-yop type iii secretion system (t3ss) encoded by a highly conserved pyv virulence plasmid to export the virulence effectors into host cells. the ysc-yop t3ss is tightly regulated by multiple contributing proteins that function at different levels. however, systematic transcriptional regulation analysis of ysc-yop t3ss is lacking and the detailed mechanism under this regulation process is still elusive. aimed at systematically characterizing transcription ...201424658611
the yersinia pseudotuberculosis complex: characterization and delineation of a new species, yersinia wautersii.the genus yersinia contains three species pathogenic for humans, one of which is the enteropathogen yersinia pseudotuberculosis. a recent analysis by multi locus sequence typing (mlst) of the 'y. pseudotuberculosis complex' revealed that this complex comprises three distinct populations: the y. pestis/y. pseudotuberculosis group, the recently described species yersinia similis, and a third not yet characterized population designated 'korean group', because most strains were isolated in korea. th ...201424598372
immunization with a dna adenine methylase over-producing yersinia pseudotuberculosis vaccine confers robust cross-protection against heterologous pathogenic serotypes.yersinia pseudotuberculosis is a foodborne pathogen that can cause serious human illness. although the source and route of transmission often remain obscure, livestock have been implicated in some cases. the diversity of yersiniae present on farms and their widespread distribution in animal and environmental reservoirs necessitates the use of broad prophylactic strategies that are efficacious against many serotypes simultaneously. herein, immunization of mice with a modified, live attenuated y. ...201424508035
yersinia pseudotuberculosis enterocolitis mimicking enteropathic γδ t-cell lymphoma with abnormal clonality.yersinia pseudotuberculosis generally infects the gastrointestinal tract and causes enteropathy symptoms suggesting infection. y. pseudotuberculosis infections are often complicated with intraceliac lymphoadenopathy mimicking malignant lymphoma. this is a first case of yersinia pseudotuberculosis enteropathy mimicking enteropathic γδ t-cell lymphoma. this case highlighted the γδ t-cell reaction to yersinia enterocolitis sometimes mimicking malignant lymphoma clinically.201424468017
yersinia pseudotuberculosis infections in goats and other animals diagnosed at the california animal health and food safety laboratory system: 1990-2012.yersinia pseudotuberculosis is a recognized zoonotic food-borne pathogen; however, little is known about the ecology and epidemiology of diseases caused by the bacterium in california. the objective of the current study was to contribute to the knowledge of the diseases caused by y. pseudotuberculosis in goats, the animal species most frequently reported with clinical yersiniosis to the california animal health and food safety laboratory system, to better understand the epidemiology of this dise ...201424442485
flis modulates flgm activity by acting as a non-canonical chaperone to control late flagellar gene expression, motility and biofilm formation in yersinia pseudotuberculosis.the flgm-flia regulatory circuit plays a central role in coordinating bacterial flagellar assembly. in this study, we identified multiple novel binding partners of flgm using bacterial two-hybrid screening. among these binding partners, flis, the secretion chaperone of the filament protein flic, was identified to compete with flia for the binding of flgm. we further showed that by binding to flgm, flis protects it from secretion and degradation, thus maintaining an intracellular pool of flgm res ...201423957589
yersinia pseudotuberculosis uses ail and yada to circumvent neutrophils by directing yop translocation during lung infection.a yersinia pseudotuberculosis (yptb) murine model of lung infection was previously developed using the serotype iii ip2666ndei strain, which robustly colonized lungs but only sporadically disseminated to the spleen and liver. we demonstrate here that a serotype ib yptb strain, ip32953, colonizes the lungs at higher levels and disseminates more efficiently to the spleen and liver compared with ip2666ndei . the role of adhesins was investigated during ip32953 lung infection by constructing isogeni ...201424119087
[elaboration of immune stimulating lipid-saponin subunit antigen carrier based on glycolipid monogalactosyldiacylglycerol from sea macrophytes and triterpene glycosides from cucumaria japonica].the ability of some triterpene glycosides of holothurians: holotoxin a1 from apostichopus japonicus and a mixture of monosulphated triterpene glycosides from cucumaria japonica called cucumarioside (cd) to form supramolecular complexes with cholesterol (chol) and monogalactosyldiacylglycerol (mgdg) or phosphatidylcholine (pc) was studied. a transmission electron microscopy method was used to observe supramolecular lipid-saponin complexes formed by holotoxin a1 and cd with cholesterol in the pres ...201425508798
[elaboration of immune stimulating lipid-saponin subunit antigen carrier based on glycolipid monogalactosyldiacylglycerol from sea macrophytes and triterpene glycosides from cucumaria japonica].the ability of some triterpene glycosides of holothurians: holotoxin a1 from apostichopus japonicus and a mixture of monosulphated triterpene glycosides from cucumaria japonica called cucumarioside (cd) to form supramolecular complexes with cholesterol (chol) and monogalactosyldiacylglycerol (mgdg) or phosphatidylcholine (pc) was studied. a transmission electron microscopy method was used to observe supramolecular lipid-saponin complexes formed by holotoxin a1 and cd with cholesterol in the pres ...201425481946
an nf-κb-based high-throughput screen identifies piericidins as inhibitors of the yersinia pseudotuberculosis type iii secretion system.the type iii secretion system (t3ss) is a bacterial appendage used by dozens of gram-negative pathogens to subvert host defenses and cause disease, making it an ideal target for pathogen-specific antimicrobials. here, we report the discovery and initial characterization of two related natural products with t3ss-inhibitory activity that were derived from a marine actinobacterium. bacterial extracts containing piericidin a1 and the piericidin derivative mer-a 2026b inhibited yersinia pseudotubercu ...201424295981
yersinia pseudotuberculosis efficiently escapes polymorphonuclear neutrophils during early infection.the human-pathogenic species of the gram-negative genus yersinia preferentially target and inactivate cells of the innate immune defense, suggesting that this is a critical step by which these bacteria avoid elimination and cause disease. in this study, bacterial interactions with dendritic cells, macrophages, and polymorphonuclear neutrophils (pmns) in intestinal lymphoid tissues during early yersinia pseudotuberculosis infection were analyzed. wild-type bacteria were shown to interact mainly w ...201424379291
negatively charged lipid membranes promote a disorder-order transition in the yersinia yscu protein.the inner membrane of gram-negative bacteria is negatively charged, rendering positively charged cytoplasmic proteins in close proximity likely candidates for protein-membrane interactions. yscu is a yersinia pseudotuberculosis type iii secretion system protein crucial for bacterial pathogenesis. the protein contains a highly conserved positively charged linker sequence that separates membrane-spanning and cytoplasmic (yscuc) domains. although disordered in solution, inspection of the primary se ...201425418176
solving the supply of resveratrol tetramers from papua new guinean rainforest anisoptera species that inhibit bacterial type iii secretion systems.the supply of (-)-hopeaphenol (1) was achieved via enzymatic biotransformation in order to provide material for preclinical investigation. high-throughput screening of a prefractionated natural product library aimed to identify compounds that inhibit the bacterial virulence type iii secretion system (t3ss) identified several fractions derived from two papua new guinean anisoptera species, showing activity against yersinia pseudotuberculosis outer proteins e and h (yope and yoph). bioassay-direct ...201425405587
high frequency of reactive arthritis in adults after yersinia pseudotuberculosis o:1 outbreak caused by contaminated grated carrots.we describe the epidemiological and microbiological process in the clearing of a foodborne outbreak of yersinia pseudotuberculosis o:1 linked to raw carrots and frequency of the associated reactive extra-gastrointestinal manifestations.201423852698
difficulties in diagnosing terminal ileitis due to yersinia pseudotuberculosis.we report three patients with terminal ileitis and positive fecal cultures with yersinia pseudotuberculosis. from one patient, a virulence plasmid (pyv)-negative y. pseudotuberculosis was isolated, which represents the second finding of a pyv-negative isolate associated with human disease. all patients were treated with ciprofloxacin and fully recovered. since conventional culture methods for yersiniosis are gradually replaced with molecular tests not recognizing y. pseudotuberculosis, we recomm ...201423925588
[statistical study of unilateral exophthalmos of an orbital cause with histological verification]. 20144290955
[statistical study of unilateral exophthalmos of an orbital cause with histological verification]. 20144290955
a clinical and polygraphic study of dreams in narcolepsy and hypersomnia. 20144183394
role of exercise tests in diagnosis of fitness in suspected heart or lung disease. 20144097610
[on the diagnosis and evaluation of hydration disorders in heart insufficiency by determination of serum osmolality of the serum sodium and the serum water levels]. 20144174679
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