Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| oral viral leukoplakia--a new aids-associated condition. | 1985 | 2994415 | |
| role of epstein-barr virus in acquired immune deficiency syndrome. | we have reviewed the biologic characteristics, immune responses, and diverse array of diseases occurring from epstein-barr virus infections in immune deficient patients. we have summarized possible roles of the virus in the risk groups for aids. data is convincing that ebv is responsible for some of the cases of lymphadenomegaly and burkitt-like, non-hodgkin's lymphomas in patients with pre-aids and aids. a hypothesis has been proposed wherein ebv and other stimulants of b and t cells allow prod ... | 1985 | 2994420 |
| lymphadenopathy morphologically consistent with hodgkin's disease associated with epstein-barr virus infection. | this case report describes a case of chronic epstein-barr virus infection resulting in lymphadenopathy morphologically indistinguishable from hodgkin's disease. morphologic, immunologic, and virologic studies could be performed on three consecutive lymph nodes. for the first time in the literature, epstein-barr nuclear antigen (ebna) could be demonstrated in the nuclei of sternberg-reed cells. | 1985 | 2994459 |
| epstein-barr virus infection in a child with acquired immunodeficiency syndrome. | a 3-year-old girl, born to an intravenous-drug-dependent mother, had protracted diarrhea, failure to thrive, generalized lymphadenopathy, and recurrent fevers during the first six months of life. at 7 months of age, the epstein-barr virus (ebv) genome was detected in her saliva by dna dot-blot hybridization using a cloned ebv probe. spontaneous ebv+ lymphoblastoid cell lines had repeatedly developed from her peripheral blood lymphocytes over the subsequent 2 1/2 years. at 11 months of age, persi ... | 1985 | 2994462 |
| nasopharyngeal carcinoma. brief review. | nasopharyngeal carcinoma has a well-defined geographic distribution, primarily affecting persons from southern china and southeast asia. environmental factors are numerous and appear to have a secondary role, mainly in the promotion of the neoplastic process. relationship with the epstein-barr virus is indicated by the identification of viral genome copies within the cells and by a persistent host antibody response with restricted specificity for nasopharyngeal malignancies. the world health org ... | 1985 | 2994473 |
| persistence of virulent canine distemper virus in lymphoblastoid cell lines. | persistent infection with virulent canine distemper virus (cdv-sh) was established in 2 human lymphoblastoid b cell lines (wi-l2 and raji), and one human (hsb), one simian (1670) and one canine (ct-45-s) lymphoblastoid t cell line. cell free virus from persistently infected t cell lines was avirulent for dogs but virulence was maintained during 31 cell passages in persistently infected b cell lines. | 1985 | 2994601 |
| effects of temperature on epstein-barr virus replication in epithelial/nasopharyngeal carcinoma hybrid cells. | the authors studied the replication of epstein-barr virus (ebv) in the epithelial hybrid cells derived from nasopharyngeal carcinoma (npckt) caused by the effects of sub-optimal incubation at 34, 32 and 28 degrees c, respectively, using an immunofluorescence technique. the intensity of ebv induction by sub-optimal temperatures was in the order of 32 degrees c----28 degrees c----34 degrees c. in particular, 18-24% positive cells for early antigen and viral capsid antigen were observed at around 1 ... | 1985 | 2994614 |
| immunofluorescence and electron microscopic investigations of epithelial hybrid cells derived from nasopharyngeal carcinoma (npc-kt). | the present study was made to investigate some characteristics of the epithelial hybrid cells derived from nasopharyngeal carcinoma (npc-kt cells) both in vivo and in vitro, using immunofluorescence and electron microscopic techniques. immunofluorescence and electron microscopic studies have shown that the appearance of epstein-barr virus (ebv)-related early antigens, eb-viral capsid antigens and virus particles in nude-mouse-grown-tumour cells were rather repressed, in contrast to, in vitro cul ... | 1985 | 2994615 |
| effects of coordinated gold compounds on in vitro and in situ dna replication. | auranofin, a coordinated gold compound, inhibits in vitro dna synthesis and displays in vivo antitumor activity. to understand the mechanisms of inhibition of dna replication, we have examined the effects of auranofin and other gold complexes on the activities of purified cellular and herpesvirus-induced dna polymerases, and on in situ dna replication in permeabilized s phase kb cells. evaluation of the data suggests the following conclusions. (1) the gold compounds varied in their abilities to ... | 1985 | 2994679 |
| different defects of t cell regulation of epstein-barr virus-induced b cell activation in rheumatoid arthritis. | several reports have shown a defective epstein-barr virus (ebv)-specific suppressor t cell function in rheumatoid arthritis (ra), suggesting that ebv may have a role in the pathogenesis of ra. ebv-specific t cell regulation was studied in 47 ebv-immune ra patients and in 14 ebv-immune control subjects by comparing the secretion of igm into supernatants of 28-day cultures of b cells alone and cocultures of b and autologous t cells. in control subjects, autologous t cells mediated a significant de ... | 1985 | 2994687 |
| immunologic and virologic status of multitransfused patients: role of type and origin of blood products. by the aids-hemophilia french study group. | an immunologic and virologic work-up was undertaken in 425 symptom-free multitransfused patients with hemophilias or hemoglobinopathies living in france. patients were entered into five groups according to the type of blood product they received: local factor viii, a mixture of local and imported factor viii, imported factor ix, local factor ix, washed red blood cells. the overall prevalence of igg antibodies to the lymphadenopathy-associated virus (lav) was 45%. the highest rate was observed in ... | 1985 | 2994780 |
| a survey of serum antibodies to eight common viruses in psychiatric patients. | serum antibody titres to eight neurotropic viruses were measured by enzyme immunoassay in 450 psychiatric in-patients and 143 controls. a seasonal variation in schizophrenic births was observed, with a peak incidence between march and april. both herpes simplex virus and cytomegalovirus antibody titres correlated with age and, when this was controlled for, no significant differences emerged between any patient group and the controls. mumps antibody titres were significantly lower in patients wit ... | 1985 | 2994791 |
| altered configuration of gc on the plasma membrane of transformed and malignant human b lymphocytes. | normal human peripheral blood b cells exhibit strong membrane fluorescence for gc (vitamin d-binding protein), and this protein can form a close spatial relationship with integral membrane immunoglobulin (mig) with evidence of codistribution in the lipid bilayer. in contrast, fluorescence for both gc and mig has been found in this study to be weak or absent in several b lymphoblastoid cell lines and in chronic lymphocytic leukemia b cells. moreover, the comobility of these components, where dete ... | 1985 | 2994927 |
| polyclonal b cell hyperplasia associated with epstein-barr virus causing acute renal allograft failure. | six weeks post cadaver renal transplantation, a patient developed a flu-like illness. acute renal failure unresponsive to anti-rejection therapy occurred and he died four days later from pneumocystis carinii pneumonia and streptococcus viridans septicemia. autopsy revealed a diffuse polymorphic polyclonal b cell infiltrate occupying most organs, including the allograft. primary epstein-barr virus (ebv) infection was established by 1) rising anti-ebv antibody titres; 2) the demonstration of ebv n ... | 1985 | 2994928 |
| lymphocyte subsets and eb virus antibodies in nasopharyngeal carcinoma. | lymphocyte subsets and epstein-barr virus (ebv)-associated antibodies were studied in 108 patients with nasopharyngeal carcinoma (npc) and in 34 normal controls. lymphocyte subsets were identified with monoclonal antibodies (ortho co.) by indirect immunofluorescent antibody (ifa) method. the helper t lymphocytes (okt4+) in npc patients comprised 38.2 +/- 10.5% which is significantly different from 45.2 +/- 8.0% in controls. the helper/suppressor ratio in npc patients was 1.33 +/- 0.65 which is s ... | 1985 | 2994964 |
| three related centromere proteins are absent from the inactive centromere of a stable isodicentric chromosome. | we developed an aqueous spreading procedure that permits simultaneous analysis of human chromosomes by q-banding and indirect immunofluorescence. using this methodology and anticentromere antibodies from an autoimmune patient we compared the active and inactive centromeres of an isodicentric x chromosome. we show that a family of structurally related human centromere proteins (cenp-a, cenp-b, and cenp-c) is detectable only at the active centromere. these antigens therefore may be regarded both a ... | 1985 | 2994966 |
| a comparison of established human lymphoma lines by flow cytometry: quantitation of ricinus communis agglutinin binding and the effect of specific glycosidases. | two established cell lines of human b-cell lymphomas derived from burkitt lymphomas and their epstein-barr virus-transformed counterparts were analyzed with respect to their ability to bind the beta-galactoside-specific lectin ricinus communis agglutinin (rca). native and sialidase- as well as sialidase-beta-galactosidase-treated cells were compared. the method for the quantitative determination of average numbers of binding sites and of apparent affinity constants was flow cytometry with fluore ... | 1985 | 2995042 |
| locus-specific detection of hla-dq and -dr antigens by antibodies against synthetic n-terminal octapeptides of the beta chain. | antibodies against synthetic peptides representing the class-ii antigen hla-dr and -dq beta chain n-terminal sequences were prepared in rabbits. the two octapeptides only share two amino acids and enzyme-linked immuno-assays showed the antisera only to bind to its own antigen. both peptide antisera detected a 29 kda component in immunoblots of raji and al-34 cell plasma membrane proteins separated by sds gel electrophoresis. the binding of either n-terminal peptide antiserum was selectively inhi ... | 1985 | 2995123 |
| [nasopharyngeal carcinoma and the epstein-barr virus]. | 1985 | 2995214 | |
| monosomy 6 in a human lymphoma line induced by selection with a monoclonal antibody. | the human epstein barr virus-superinfected b lymphoma cell line bjab-b95.8.6 was mutagenized by gamma irradiation, and hla mutants were selected with the hla-bw6-specific monoclonal antibody sfr8-b6. one of the mutants obtained, bm19, had lost one of the chromosomes 6 present in the wild type cells. electrophoretic analysis of phosphoglucomutase isozyme pgm3 and erythrocyte glyoxalase 1 from both cells supports this conclusion. the hla antigens expressed on bm19 were hla-a2, b13, bw4, c-, dr2 (q ... | 1985 | 2995242 |
| structure-activity relationship in the induction of epstein-barr virus by teleocidin derivatives. | new derivatives of (-)-indolactam v (fig. 1), which have the basic ring-structure of teleocidins without the monoterpenoid moiety, were prepared and their epstein-barr virus early antigen (ebv-ea)-inducing activity was tested. (-)-14-o-alkyl indolactam vs (2 and 3) showed little induction of ebv-ea, while (-)-14-dehydroxyindolactam v (4) and (-)-14-chloroindolactam v (5) proved to be potent ebv-ea inducers, though their activities (ec50) were about 10 times weaker than that of (-)-indolactam v ( ... | 1985 | 2995261 |
| epstein-barr virus serology in nasopharyngeal carcinomas and other head and neck neoplasms in italy. | this paper reports the results of the ebv-specific antibody response in 17 italian nasopharyngeal carcinoma (npc) patients, 15 other head and neck tumor patients and 15 normal controls. nucleic acid hybridization has been performed on the biopsy tissue from 4 of the npc patients, and ebv-dna was present in two undifferentiated (who 3) tumors, and absent in two samples of the keratinizing (who 1) type. ebv serology appears to be specifically related to npc, more evidently for vca-iga and ea-igg a ... | 1985 | 2995399 |
| circulating activated b cells in primary biliary cirrhosis. | since patients with primary biliary cirrhosis (pbc) have evidence of abnormal function of the humoral immune system, we determined if b cells from patients with this disease show evidence of activation and can be stimulated by polyclonal activators. using a reverse hemolytic plaque assay, it was found that patients with pbc had a significant increase in the number of circulating immunoglobulin-secreting cells, compared to normal controls and patients with chronic type b hepatitis virus (hbv) inf ... | 1985 | 2995432 |
| immunological and virological investigation in patients with lymphoadenopathy syndrome and in a population at risk for acquired immunodeficiency syndrome (aids), with particular focus on the detection of antibodies to human t-lymphotropic retroviruses (htlv iii). | thirteen patients affected with unexplained lymphoadenopathy, fever, weight loss, and diarrhea (lymphoadenopathy syndrome; las) were evaluated for the possible appearance of acquired immunodeficiency syndrome (aids) and for immunological and virological characterization. the patients belonged to categories of individuals at risk for aids and were homosexual and/or drug abusers or hemophiliacs. lymph node biopsy showed the histological picture of a follicular hyperplasia. the study of cell-mediat ... | 1985 | 2995433 |
| specificity repertoire of lymphocytes from multiple myeloma patients. i. high frequency of b cells specific for idiotypic and f(ab')2-region determinants on immunoglobulin. | the specificity repertoire of b lymphocytes from 14 multiple myeloma patients has been studied using the technique of epstein-barr virus (ebv) transformation of peripheral blood lymphocytes (pbl) coupled with clonal analysis by limiting dilution. we find that up to 100% of the b cells from myeloma patients undergoing ebv transformation secrete igm specific for determinants on the f(ab')2 region of autologous and/or heterologous monoclonal immunoglobulin. in normal individuals 0.02-0.73% of the t ... | 1985 | 2995434 |
| fluoroimmunoassay for detection of rubella-specific immunoglobulin m: comparison with indirect enzyme immunoassay and mu-chain capture. | the performance of a commercially-available method of fluoroimmunoassay (rubella m fiax, international diagnostic technology, santa clara, calif.), designed for the detection of rubella-specific immunoglobulin m, was tested with 137 selected sera, including 52 from cases of primary rubella, 29 from healthy pregnant women, 21 containing rheumatoid factor, and 35 from cases of infectious mononucleosis (im) caused by epstein-barr virus. the results were compared with those obtained by commercial in ... | 1985 | 2995439 |
| binding of monoclonal antibody to the epstein barr virus (ebv)/cr2 receptor induces activation and differentiation of human b lymphocytes. | a panel of b cell-specific monoclonal antibodies that identify the cr2/ebv receptor were examined for their ability to mimic the t-independent mitogenic agent, ebv, and thus activate human peripheral blood b lymphocytes. two of four different anti-cr2/ebv monoclonal antibodies, okb7 and ab-1, produced a 50-fold to 200-fold dose-dependent stimulation of dna synthesis of peripheral blood mononuclear cells. one of the other monoclonal antibodies, anti-b2, had slight activity, and the other, hb-5, w ... | 1985 | 2995485 |
| il 1-like activity in antigen-presenting human b cell lines. | the secretion of interleukin 1 (il 1) by an antigen-presenting cell (apc) may be essential to its function in the stimulation of t cell responses. however, the relevance of il 1 is less clear in cases where the apc are from continuous b cell lines. we have shown that il 1-like activity can be demonstrated in human b cell lines by using a cellular co-culture assay for il 1. significant il 1 activity could not be detected in the supernatants of these b cell lines produced either constitutively or ... | 1985 | 2995488 |
| epstein-barr virus infections and dna hybridization studies in posttransplantation lymphoma and lymphoproliferative lesions: the role of primary infection. | fourteen patients who developed b cell lymphomas or lymphoproliferative lesions after kidney, liver, heart, or heart-lung transplantation in pittsburgh during 1981-1983 had active infection with epstein-barr virus (ebv) of the primary (six patients), reactivated (seven patients), or chronic (one patient) type. in transplant patients without tumors, the incidence of ebv infection was 30% (39 of 128). only three of these patients had primary infections. thus the frequency of active infection was s ... | 1985 | 2995512 |
| antibody to epstein-barr virus-specific dnase as a marker for the early detection of nasopharyngeal carcinoma. | we have previously shown that high levels of antibody to epstein-barr virus (ebv)-specific dnase may be a useful marker for the early diagnosis of nasopharyngeal carcinoma (npc). sera from 3,368 males in an area with a high risk for the development of npc were examined for the presence of antibody to ebv-specific dnase activity. significant levels of the antibody were found in 430 of these sera. as a result, 32 individuals have attended the out-patient department of otolaryngology for clinical e ... | 1985 | 2995572 |
| epstein-barr virus serology and malaria exposure in a small group of liberian children with splenomegaly. | 1985 | 2995691 | |
| retrovirus and malignant lymphoma in homosexual men. | we report malignant lymphoma in 27 homosexual men, of whom 22 had high-grade lymphomas (b-cell immunoblastic sarcoma or small non-cleaved lymphoma) and five had low-grade disease. antibody to human t-cell lymphotropic virus type iii (htlv-iii) was present in 13 (87%) of 15 with high-grade lymphoma and in two (40%) of five with low-grade disease. in contrast, only one (9%) of 11 "control" heterosexual patients with high-grade lymphoma had antibody to htlv-iii, while such antibody was found in non ... | 1985 | 2995692 |
| [a patient with immunoblastic lymphadenopathy: possible relation to epstein-barr virus infection]. | 1985 | 2995700 | |
| association of epstein-barr virus with lethal midline granuloma. | 1985 | 2995813 | |
| head and neck oncology--1985. reflections on changing times. | the field of head and neck oncology is keeping pace with the dramatic shift in cancer treatment modalities occurring during this period of medical history. having achieved a level of surgical elegance, the head and neck oncologist has the future to rely upon for more biological methods of management of this disease. biochemistry, immunology, and virology occupy much of the research interest pertaining to malignancies of these body systems and it is within this research that the answers will prob ... | 1985 | 2995898 |
| nasopharyngeal carcinoma. clinical presentation, diagnosis, treatment, and prognosis. | serologic testing is a useful diagnostic aid for patients with npc, particularly those in whom the tumors are small and submucosal (difficult to see or occult). if a metastatic tumor is found in the neck but its primary source is occult, positive titers provide reason for a detailed investigation of the nasopharynx, including a thorough examination with the patient under anesthesia and a random biopsy procedure. this approach can spare the patient a biopsy of neck nodes. dickson compared two gro ... | 1985 | 2995899 |
| squamous cell carcinoma. metastasis to the neck from an unknown or undiscovered primary. | with an unknown or occult primary cancer, the clinical presentation can be either undiagnosed lump or lumps in the neck that has been biopsied and diagnosed as metastatic cancer on the basis of the histopathologic findings. | 1985 | 2995900 |
| effects of human interferon on cell proliferation and antigens induced by epstein-barr virus in epithelial hybrid cells derived from nasopharyngeal carcinoma. | the effects of human leukocyte interferon (hulfn-alpha) and fibroblast interferon (hulfn-beta) on cell growth and induction of antigens related to epstein-barr virus (ebv) in a nasopharyngeal carcinoma hybrid cell line (npc-kt) were studied. hulfn-alpha showed little antiproliferative and anti-ebv effect on npc-kt cells. on the other hand, hulfn-beta showed modest antiproliferative effect on npc-kt cells; the effect was dependent on time and hulfn-beta concentration. hulfn-beta alone was ineffec ... | 1985 | 2995902 |
| clinical manifestations of herpesvirus infections in pediatric renal transplant recipients. | we report our experience with 29 symptomatic herpesvirus infections occurring during the course of 87 pediatric transplant procedures performed over the 10-year period, 1973 to 1982. the yearly attack rate ranged from 0.05 to 0.40 case per cumulative patient years at risk. a greater proportion (9 of 14) of children who received more than 10 units of whole blood or packed red blood cells prior to transplantation developed a viral infection compared with those given 10 transfusions or fewer (8 of ... | 1985 | 2995934 |
| in vitro growth characteristics of simian t-lymphotropic virus type iii. | the type c retrovirus simian t-lymphotropic virus type iii (stlv-iii) has been isolated recently from immunodeficient macaque monkeys at the new england regional primate research center. the present studies were done to define the in vitro growth characteristics of this agent. stlv-iii replicates efficiently in interleukin 2-dependent t-cell cultures of macaque peripheral blood lymphocytes (pbl), less efficiently in such cultures of human and gibbon pbl, and inefficiently in baboon pbl. no repli ... | 1985 | 2996002 |
| functional relation between htlv-ii x and adenovirus e1a proteins in transcriptional activation. | the mechanism of cellular transformation by the human t-cell leukemia viruses (htlv) is thought to involve a novel gene known as the x gene. this gene is essential for htlv replication and acts by enhancing transcription from the htlv long terminal repeat. the htlv x gene product may also cause aberrant transcription of normal cellular genes, resulting in transformation of the infected cells. although there is no evidence as yet for such a mechanism, it was shown that the htlv-ii x gene product ... | 1985 | 2996140 |
| hla typing of epstein-barr virus transformed lymphoblastoid cell lines (lcl). | the application of standard tissue typing techniques to cells other than peripheral blood lymphocytes has been accompanied by the problem of extra reactions. this applies as well to epstein-barr virus transformed lymphoblastoid cell lines (lcl) as to leukemic cells and human spleen cells. these extra reactions are attributable to additional antibodies in the typing sera which are not apparent under standard conditions with pbls. two types are described: type 1 extras, which becomes apparent afte ... | 1985 | 2996171 |
| a new approach to the diagnosis of active rh0(d) immunization in passively immunized pregnant women. | an assay has been developed to distinguish active from passive rh0(d) immunization in a patient who had recently received hyperimmune anti-rh0(d) immunoglobulin therapy. isolated peripheral b lymphocytes from a pregnant woman at 32 weeks gestation were co-cultured with epstein-barr virus in a liquid growth medium. after 7 days, anti-rh0(d) antibodies produced in vitro by the transformed lymphocytes were detected in culture supernatants, thereby proving active immunization and indicating the pote ... | 1985 | 2996227 |
| [immunoglobulin a serum antibodies against the capsid antigen of epstein-barr virus in the differential diagnosis and follow-up of nasopharyngeal cancer]. | iga antibodies to epstein-barr virus capsid antigen (iga anti-vca) can be detected in sera of patients with certain types of nasopharyngeal carcinoma (npc). iga anti-vca titres were determined by the indirect immunofluorescence technique. 17 control patients with benign diseases or carcinomas of the head and neck other than npc had negative iga anti-vca titres less than or equal to 1:16. npc was diagnosed histologically according to the cologne modification of the who classification. among 16 ca ... | 1985 | 2996246 |
| epstein-barr virus infection and serological profile in greenland eskimo children. | the epstein-barr virus (ebv)-specific antibody profile of 101 greenland eskimo children was determined. the proportion of children with serological evidence of recent or past primary ebv infections rose from 22% at 6 months of age to 79% at 24 months of age. all but 2 of 49 children more than 4 years of age proved seropositive. the geometric mean titre (gmt) of antibodies to the viral capsid antigen (vca) was highest during the first 3 years of life and declined sharply to a lower, nearly consta ... | 1985 | 2996288 |
| diagnosing infectious mononucleosis. | infectious mononucleosis can be diagnosed with certainty only when suggestive clinical findings are corroborated by relative and absolute lymphocytosis, lymphocyte atypia of more than 20 percent and a positive serologic test. a serologic test is not absolutely necessary when atypical lymphocytes exceed 40 percent since this is specific to infectious mononucleosis. the diagnosis is difficult when clinical findings are scanty and the blood picture is unhelpful. | 1985 | 2996323 |
| production of a human monoclonal antibody to hla by human-human hybridoma technology. a preliminary report. | an epstein-barr-virus-transformed lymphoblastoid cell line (ecebv) was derived from a multiply transfused renal dialysis patient. ecebv was shown to secrete specific antibody in a cellular enzyme-linked immunosorbent assay (celisa) and was hybridized with the mutagenized human fusion partner g m1500 resistant to 6-thioguanine and ouabain. hybridomas surviving hypoxanthine-aminopterin-thymidine (hat) and ouabain selection were cloned by limiting dilution. the hybridomas continue to secrete antibo ... | 1985 | 2996358 |
| incidence of involvement of the b and t lymphocyte lineages in chronic myelogenous leukemia. | peripheral blood specimens were obtained from 22 patients with philadelphia chromosome (ph1) positive chronic myelogenous leukemia (cml) (16 in chronic phase, 2 in an accelerated phase, and 4 in blast crisis). studies were performed to determine the frequency of the presence of the ph1 chromosome in cells of lymphoid lineages. rosetted (e+) lymphocytes (t lymphocytes) from nine patients in chronic phase and one patient in blast crisis were stimulated with t cell growth factor interleukin 2 (il-2 ... | 1985 | 2996659 |
| herpes viruses: a background. | 1985 | 2996684 | |
| epstein-barr virus-inducing activity of euphorbiaceae plants commonly grown in cameroon. | epstein-barr virus (ebv) activating potency of 12 species of plants belonging to the euphorbiaceae family, that are commonly grown in cameroon, one of the endemic areas of burkitt's lymphoma (bl), was investigated. the ebv-inducing activity was found in most of the plants tested and in the soil around the plants whose root extracts were active. these findings support the notion that such ebv-activating principles are one of the environmental co-factors causing bl. | 1985 | 2996755 |
| epstein-barr virus activation by human semen principle: synergistic effect of culture fluids of bacteria isolated from patients with carcinoma of uterine cervix. | epstein-barr virus early antigens (ebv ea) were induced in a raji cell system in order to assay the activity of the ea inducer in human semen. in semen from 53 chinese, 45.3% induced ebv ea in raji cells. such positive semen and ebv-inducing positive culture fluids of bacteria isolated from the uterine cervix of patients with cervical carcinoma had a synergistic effect on the induction of ebv ea. this synergistic effect as related to the cause of cervical carcinoma is discussed. | 1985 | 2996756 |
| sequence-specific dna binding of the epstein-barr virus nuclear antigen (ebna-1) to clustered sites in the plasmid maintenance region. | latently infected b lymphocytes continuously express an epstein-barr virus nuclear antigen (ebna-1) required in trans for maintenance of the plasmid state of the ebv genome. filter binding assays and dnaase i footprinting analyses revealed that the carboxy-terminal domain of ebna-1 protects binding sites at three different loci in the 172,000 bp ebv genome. two of these loci correspond to essential elements within an 1800 bp segment defined as the minimal region required for plasmid maintenance ... | 1985 | 2996781 |
| virologic and immunologic study on acquired immune deficiency syndrome. | 1985 | 2996840 | |
| hemophiliacs and acquired immune deficiency syndrome. | 1985 | 2996841 | |
| [the relationship between epstein-barr virus and rheumatoid arthritis]. | 1985 | 2996850 | |
| serological diagnosis of acute viral hepatitis. | fifty cases of symptomatic acute viral hepatitis presenting at the washington, d.c., veterans administration medical center between 1976 and 1978 were tested for serological markers of hepatitis virus infection. the etiology of the acute hepatitis appeared to be hepatitis a virus in 20%, hepatitis b virus in 52%, non-a, non-b agents in 22%, delta hepatitis in 4%, and infectious mononucleosis in 2%. the diagnosis of type b hepatitis was difficult to verify because 10% of cases were seronegative f ... | 1985 | 2996863 |
| epstein-barr virus antibodies in neurological diseases. | antibody titers to the epstein-barr virus (ebv) in the serum and cerebrospinal fluid (csf) were determined in 57 cases of acute or subacute neurological diseases. as a result, sera from 11 cases (7 bell's palsy, 2 encephalitis and 2 acute cerebellar ataxia) were found to be positive for antibodies to early antigen. seven of these 11 cases either seroconverted for igg antibodies to viral capsid antigen (vca) or were proven positive for anti-vca-igm antibodies in the serum. while 4 were found posi ... | 1985 | 2997009 |
| autocrine models of b-lymphocyte growth. i. role of cell contact and soluble factors in t-independent b-cell responses. | the requirements for triggering human b cells to dna synthesis by t-independent polyclonal activators were examined. optimal s phase entry of purified resting b cells infected with epstein-barr virus (ebv) or confronted with killed particles of staphylococcus aureus cowan strain i (sac) required a high density of cells in culture. experiments varying culture vessel geometry and culture volumes revealed that the initial limiting quantity was a soluble activity generated in the b-cell cultures. a ... | 1985 | 2997026 |
| humoral immune response in infectious mononucleosis. late emergence of anti-ea(r) and the effects of corticosteroid therapy. | the antibody response to epstein-barr virus (ebv) antigens in patients with infectious mononucleosis (im) was studied to assess antibody appearance to the restricted (r) component of the early antigen (ea) complex and to determine the effect of corticosteroids on all aspects of the humoral immune response. sixty college students with heterophil-positive clinical im, confirmed by ebv-specific serology, were followed for a period of 4-26 weeks, half received prednisone for six days, and the remain ... | 1985 | 2997092 |
| human t-cell leukemia/lymphoma virus i and/or epstein-barr virus-infected b-cell lines spontaneously produce acid-labile alpha-interferon. | b-cell lines were established as spontaneous outgrowths of cell cultures from patients with adult t-cell leukemia (atl). three such lines were shown to have integrated human t-cell leukemia/lymphoma virus 1 (htlv-i) proviral sequences as well as epstein-barr virus (ebv) infection. supernatant fluids from these cultured cells were assayed for interferon (ifn) production. acid-stable alpha-ifn was found to be produced by one cell line (cf), and acid-labile alpha-ifn by the other two (hs, mjb). in ... | 1985 | 2997264 |
| low expression of human histocompatibility leukocyte antigen-dr is associated with hypermethylation of human histocompatibility leukocyte antigen-dr alpha gene regions in b cells from patients with systemic lupus erythematosus. | the relationship between the expression of hla-dr antigens and the hla-dr alpha gene methylation was examined in systemic lupus erythematosus (sle). using permanent b cell lines, we found reduced dr expression in sle. the low dr expression was correlated with high anti-dna antibody titers in patients' sera. the amounts of dr alpha message were lower in sle cells than in normal controls, suggesting that the low expression of dr antigens is associated with gene functions. the extent of dna methyla ... | 1985 | 2997276 |
| analysis of the genomic termini of tupaia herpesvirus dna by restriction mapping and nucleotide sequencing. | a recombinant plasmid harboring both genomic termini of tupaia herpesvirus (thv) dna was characterized by restriction enzyme analysis and by determination of the nucleotide sequence. a unique noti cleavage site was found that is located approximately 19 base pairs upstream of the thv terminal junction. thv dna fragments from virion dna were analyzed by using the same restriction enzymes as for the recombinant plasmid. the comparative fine mapping of virion thv dna revealed heterogeneous molecule ... | 1985 | 2997469 |
| osteomas in of-1 mice: no alteration in biologic behavior during long-term treatment with cyclosporine. | during the course of a long-term oral study in of-1 mice for the assessment of any carcinogenic potential of the immunosuppressive agent cyclosporine (cs-a), a high incidence of osteomas was found in all treatment groups as well as in controls. the incidences of osteoma-bearing mice were 20% in control male and 30% in control female mice; the respective incidences in all treated mice were 14.7% for males and 38% for females. the osteomas were found to occur in multiple sites in 70% of control ma ... | 1985 | 2997534 |
| detection of environmental effects through anatomic pathology. | anatomic pathology has produced considerable knowledge about environmental teratogens and carcinogens. a special disease registry established by a pathologist provided details of the association between oral contraceptives and hepatic neoplams. pathologists were also involved in establishing in the link between diethylstilbestrol use and clear-cell adenocarcinomas of the vagina. an area of particular interest has been gender and ethnic differences in the incidence of certain diseases. pathol ... | 1985 | 2997598 |
| herpesvirus infections of pregnancy. part i: cytomegalovirus and epstein-barr virus infections. | 1985 | 2997607 | |
| the t4 lymphocyte in aids. | 1985 | 2997611 | |
| epstein-barr virus-positive burkitt's lymphoma cells not recognized by virus-specific t-cell surveillance. | the pathogenesis of epstein-barr (eb) virus-positive burkitt's lymphoma (bl) appears to involve the combined actions of virus-induced b-cell proliferation, and a rare chromosomal translocation juxtaposing c-myc and immunoglobulin gene loci in a single b cell; holoendemic malarial infection in some way facilitates the oncogenic process. outgrowth of the eb virus-positive tumour suggests either breakdown or evasion of those immune controls, in particular cytotoxic t-cell responses against the viru ... | 1985 | 2997615 |
| [complications of infectious mononucleosis in childhood]. | 1985 | 2997689 | |
| [the role of cytogenetics in malignant proliferations]. | 1985 | 2997769 | |
| identification and expression of a nuclear antigen from the genomic region of the jijoye strain of epstein-barr virus that is missing in its nonimmortalizing deletion mutant, p3hr-1. | an epstein-barr virus (ebv) deletion mutant, hr-1, cannot immortalize lymphocytes. hr-1 was derived from a virus strain, jijoye, that is immortalization competent. using human antiserum from certain patients with chronic active ebv infection, we have identified in jijoye cells a protein of apparent mass of 78-80 kda that is missing in cells with the hr-1 genome. a protein of identical size and antigenicity has been stably expressed in mouse ltk- cells by gene transfer with cloned jijoye ebv dna ... | 1985 | 2997790 |
| structure of the epstein-barr virus nuclear antigen as probed with monoclonal antibodies. | five hybridoma cell lines secreting antibodies directed against an epstein-barr virus (ebv) nuclear antigen (bamhi k antigen) have been isolated. all five antibodies detect this antigen in a variety of ebv-positive lymphoblastoid cell lines. the reaction of these antibodies with several mutant forms of this protein has allowed the antibody binding site(s) to be predicted. | 1985 | 2997981 |
| induction of a deoxyuridine triphosphate nucleotidohydrolase activity in epstein-barr virus-infected cells. | superinfection of raji cells with epstein-barr virus (ebv) or chemical induction of hr-1 cells with 12-o-tetradecanoyl-phorbol-13-acetate (tpa) results in the induction of a deoxyuridine triphosphate nucleotidohydrolase (dutpase) which is not observed in mock-treated cells or tpa-treated ebv genome-negative bjab cells. the ebv-induced dutpase could be distinguished from the host dutpase based upon differences in their migration in polyacrylamide gels and sensitivity to the 5-mercurithioguanosine ... | 1985 | 2997989 |
| an identification of a transforming region of epstein-barr viral dna cannot be confirmed. | we have analyzed epstein-barr viral dna sequences in two cell lines hi-26-36 and hi-hfx. stoerker et al. (j. stoerker, j.e. holliday, and r. glaser (1983), virology 129, 199-206) concluded that these cells were transformed by virus that arose as the result of marker rescue of a transformation-incompetent deletion mutant of ebv. we compared viral dna sequences in prototype ebv strains with data presented in the virology paper. our findings are not consistent with the data of stoerker et al. and i ... | 1985 | 2998023 |
| the short unique region of the b95-8 epstein-barr virus genome. | the 12-kbp short unique region of the b95-8 epstein-barr virus (ebv) genome has been sequenced and analysed for latent and lytic cycle transcripts. two latent and three late mrnas have been detected, the largest of the late transcripts potentially encoding a 143-kda protein. the region containing orip, the putative origin of replication of the genome as a plasmid in latently infected b lymphocytes, is shown to contain 21 direct repeats of a 30-bp a+t-rich sequence and a related large inverted re ... | 1985 | 2998073 |
| the bamhi f region of the b95-8 epstein-barr virus genome. | the bamhi f region of the b95-8 epstein-barr virus (ebv) genome has been sequenced and analysed for transcription signals and open reading frames. s1 mapping and northern blotting with probes from m13 recombinants have been used to search for mrnas. four rightward-reading frames encoding basic proteins appear to be expressed by 3'-coterminal early mrnas. two leftward-reading frames appear to be expressed by 3'-coterminal early mrnas. | 1985 | 2998075 |
| transient passovoy defect during a febrile illness. | the passovoy defect is a recently characterized hemorrhagic diathesis. we describe a patient with a febrile illness, possibly from epstein-barr (eb) virus, who acquired this defect transiently. prothrombin time; assays for factors viii, ix, xi, xii; and fletcher (prekallikrein) and fitzgerald (high molecular weight kininogen) factors were normal. no definite circulating inhibitor could be demonstrated. the transient passovoy defect could possibly be ascribed to the infectious process or sulfisox ... | 1985 | 2998185 |
| chronic epstein-barr virus disease: a workshop held by the national institute of allergy and infectious diseases. | 1985 | 2998253 | |
| a seroepidemiological study of cytomegalovirus and epstein-barr virus in rheumatoid arthritis and sicca syndrome. | antibodies to cytomegalovirus (cmv) and epstein-barr virus capsid antigen (ebvca) were examined in 41 patients with rheumatoid arthritis (ra), 26 patients with primary sicca syndrome, and 26 healthy subjects of similar age and sex. igg antibody titres to ebvca and cmv were similar in all three groups, apart from a trivial increase of antibodies to ebvca in ra. false positive igm anti-cmv antibodies detected in serum from one patient with sicca syndrome and 20 patients with ra were shown to be du ... | 1985 | 2998290 |
| ultrastructural studies on cell fusion induced by epstein-barr virus or n-butyrate and 12-o-tetradecanoylphorbol-13-acetate. brief report. | the morphological changes which occur in raji cells during ebv induced fusion are described. of particular interest is the formation of local contacts between cells, at these points the plasmalemmae of the two cells become disorganized and cytoplasmic bridges are formed. | 1985 | 2998304 |
| the expression of ia-antigen on nasopharyngeal carcinomas xenografted into nude mice. | the expression of ia-antigen on four different epstein-barr virus associated nasopharyngeal carcinomas xenografted into athymic mice could be detected by the monoclonal antibody okia. xenografts of four additional head and neck tumors other than nasopharyngeal carcinoma and one xenograft of a metastatic melanoma cell line were negative for the ia-antigen. control antibodies okt3, okt4, okt9, okm1 and leu7 were negative with all nasopharyngeal carcinomas and the non-nasopharyngeal carcinoma xenog ... | 1985 | 2998311 |
| melphalan-resistant lymphoblastoid cell lines established from patients with ovarian cancer treated with cross-linking agents. | lymphoblastoid cell lines (lcls) were established from 83 patients with ovarian cancer by transformation of peripheral b lymphocytes with epstein-barr virus. comparing the melphalan resistance of different groups of lcls using the mean do obtained from clonogenic survival assays, lcls from melphalan-treated patients were significantly more resistant than lcls from patients not treated with this drug. however, prior treatment of the patient with ionizing radiation was not associated with increase ... | 1985 | 2998314 |
| anti-rabbit thymus actin antibody inhibits proliferation of epstein-barr virus-transformed human b cell line la350: augmentation by cyclic amp. | antibody to actin isolated from the rabbit thymus gland exerted a dose-dependent inhibitory effect upon the proliferation of an epstein-barr virus-transformed human b cell line, la350. purified rabbit thymus actin specifically reversed the inhibitory effect of antibody by competing with surface actin on la350. for example, la350 proliferation at 48 hr was inhibited 90% at a 1:10 antibody dilution (p less than 0.001) but only 48% and 20% in the presence of 190 and 380 micrograms/ml of rabbit thym ... | 1985 | 2998386 |
| establishment of a novel human megakaryoblastic leukemia cell line, meg-01, with positive philadelphia chromosome. | a megakaryoblastic cell line, designated meg-01, was established from the bone marrow of a patient with blast crisis of philadelphia (ph1) chromosome-positive chronic myelogenous leukemia. meg-01 cells grew in single-cell suspension with a doubling time of 36 to 48 hours. under the usual culture conditions, approximately half of the cells adhered to the culture flask with extention of pseudopods. meg-01 cells were positive for the periodic acid-schiff reaction, alpha-naphthyl acetate esterase, a ... | 1985 | 2998511 |
| a p50 surface antigen restricted to human urinary bladder carcinomas and b lymphocytes. | we have previously described the derivation of a monoclonal antibody, s2c6, to a novel 50 kdalton antigen associated with human urinary bladder carcinoma. no reactions were obtained with carcinomas of unrelated origin or with normal urothelial cells. however, the antibody also reacted with a similar antigen on some cell lines of b lymphocyte origin. using large panels of target cells we have now shown that this reactivity was entirely restricted to cells of the b lineage within the haematopoieti ... | 1985 | 2998589 |
| production of a monoclonal antibody to a membrane antigen of human t-cell leukaemia virus (htlv1/atlv)-infected cell lines from a systemic lupus erythematosus (sle) patient: serological analyses for htlv1 infections in sle patients. | human t-cell leukaemia virus (htlv1/atlv), which causes adult t cell leukaemia (atl), is an infectious, lymphotrophic retrovirus unique for humans. the present study was undertaken to determine whether htlv1 had any pathogenetic role for systemic lupus erythematosus (sle). the incidence of antibodies to atl cell-associated antigens (atla) in sera from patients with sle and other collagen diseases was investigated by an indirect immunofluorescent cytoplasmic staining of an htlv1-infected cell lin ... | 1985 | 2998659 |
| self-reactive b lymphocytes detected in young adults, children and newborns after in vitro infection with epstein-barr virus. | b-lymphocytes from healthy children and young adults who were seronegative for autoantibodies and b lymphocytes from umbilical cord blood of newborns were induced to secrete a variety of autoantibodies upon infection with epstein-barr virus. such autoantibody-secreting clones were obtained from different lymphoid tissues and occurred at frequencies of 1 in 10(6)-10(7) mononuclear cells. the autoantibodies were exclusively of the igm class. they recognized normal cellular components, such as cyto ... | 1985 | 2998660 |
| efficient expression of an epstein-barr nuclear antigen in drosophila cells transfected with epstein-barr virus dna. | in a search for exogenous promoters which function in cultured drosophila cells, we have co-transfected a d. melanogaster cell line with an epstein-barr virus (ebv) cosmid clone which encodes the epstein-barr nuclear antigen (ebna-1). here we report that drosophila cells containing stably integrated copies of ebna-1 encoding dna synthesise a polypeptide of mol. wt. identical to that of authentic ebna-1, which is detectable with ebna-positive but not ebna-negative human serum. as in ebv-transform ... | 1985 | 2998769 |
| ebv-inducing factor from platelets exhibits growth-promoting activity for nih 3t3 cells. | an epstein-barr virus-indicating factor (eif) has been purified from serum and platelets. we show here that highly purified preparations of platelet eif exhibit growth-promoting activity for nih 3t3 cells maintained in platelet-poor plasma. the epstein-barr virus (ebv)-inducing activity and growth-promoting activity co-elute upon gel chromatography under non-dissociating as well as dissociating conditions and co-migrate in sds-gel electrophoresis, supporting the notion that both activities resid ... | 1985 | 2998777 |
| burkitt's lymphoma in europe. | burkitt's lymphoma (bl) is the most frequent childhood non-hodgkin's malignant lymphoma (nhml) in europe and accounts for 5 to 10% of adult nhml. age distribution is similar to that of endemic bl, with a male:female ratio of 3.7:1. epstein-barr virus (ebv) association is found in 15% of cases. a better definition of this monoclonal b-cell malignant proliferation is cytogenetic (i.e., 8;14 or variant translocation). abdominal masses are initially present in 70% of cases, whereas the jaw is involv ... | 1985 | 2998984 |
| the beginnings of the burkitt's lymphoma story. | 1985 | 2998985 | |
| clinical features of burkitt's lymphoma in the usa. | 1985 | 2998986 | |
| epstein-barr virus and burkitt's lymphoma worldwide: the causal relationship revisited. | burkitt's lymphoma (bl) in tropical africa represents by far the most common tumour in children between 0 and 14 years of age, 97% of the tumours being associated with epstein-barr virus (ebv). in north africa, the tumour is about ten times less frequent than in equatorial africa, but, according to reports from algeria, 85% of the cases appear to be associated with ebv. in western countries, bl represents about 3% of childhood tumours, 10 to 15% of them ebv-associated. thus, from the northern in ... | 1985 | 2998987 |
| historical background; burkitt's lymphoma and epstein-barr virus. | 1985 | 2998988 | |
| epidemiology of burkitt's lymphoma: other risk factors. | events surrounding infection and disease in infectious mononucleosis are a model for epidemiological factors that may affect the phase of initiation, the phase of promotion and the phase of tumour development in african burkitt's lymphoma (bl). consideration of the circumstances of early epstein-barr virus (ebv) infection as the initiator, and of malaria as the promoter, of african bl reveal some shortcomings in the evidence incriminating malaria. possible factors leading to the emergence of the ... | 1985 | 2998989 |
| a preliminary report of epidemiological studies of burkitt's lymphoma, epstein-barr virus infection and malaria in north mara, tanzania. | 1985 | 2998990 | |
| role of epstein-barr virus in the etiology of burkitt's lymphoma. | although epstein-barr virus (ebv) was discovered in cultured burkitt's lymphoma (bl) cells, its exact role remains unclear. viral genome is found in 95-98% of endemic bl and 15-20% of non-endemic bl. children destined to develop bl in africa show elevated titres of viral capsid antibodies one to two years preceding emergence of bl. a multistep process follows early ebv infection during early childhood. immune deficiency probably permits continuation of the infections, with smouldering polyclonal ... | 1985 | 2998991 |
| cell-mediated immunosurveillance mechanisms and the pathogenesis of burkitt's lymphoma. | paired epstein-barr virus (ebv)-carrying cell lines have been established from burkitt's lymphoma (bl) patients, one of each pair being the bl cell line derived from the malignant cells of the tumour, the other, the lymphoblastoid cell line (lcl) derived from the patient's normal b cells by experimental infection with the virus. comparative studies have shown the following: (1) all the lines were to some extent sensitive to in-vitro activated natural-killer cells, individual pairs differing as t ... | 1985 | 2998992 |
| the cytogenetics of human b lymphoid malignancy: studies in burkitt's lymphoma and epstein-barr virus-transformed lymphoblastoid cell lines. | cells from burkitt's lymphoma (bl) and from the majority of human b-cell neoplasms show karyotypic changes that characteristically involve chromosomal breakage and recombination in addition to some chromosome gains. these aberrations increase as the tumours progress in vivo, and a similar tendency is seen in bl-derived lymphoid lines in vitro. epstein-barr virus (ebv)-transformed lymphoblastoid lines of non-malignant origin also develop karyotypic abnormalities on prolonged culture, but these ar ... | 1985 | 2998993 |
| prevention of endemic burkitt's lymphoma. | 1985 | 2998994 | |
| biochemistry of latent epstein-barr virus infection and associated cell growth transformation. | there is sufficient knowledge of the biochemistry of epstein-barr virus (ebv) persistence and gene expression in latent growth-transforming infection and of the persistence and expression of other oncogenic viruses to permit interesting and possibly useful comparisons. most smaller oncogenic viral genomes usually persist solely as integrated dnas despite their ability to circularize. papilloma and hepatitis viruses may persist as episomes, and parts of their genomes may integrate. usually, only ... | 1985 | 2998995 |