Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| cryptosporidium infection in children with diarrhoea of acute onset. | 2002 | 12164606 | |
| cyst production and transmission of entamoeba and endolimax. | we studied the cyst production of 340 asymptomatic carriers with single or mixed infections of entamoeba histolytica/entamoeba dispar, endolimax nana and entamoeba coli. it was found that the main source of transmission was a small group of 37 (10.9%) carriers who produced 70.2% of all cysts. gender was an important factor for cyst production of ent. histolytica/ent. dispar, since males produced 6-fold more cysts than females. females produced 'large' (13-15 microns) ent. histolytica/ent. dispar ... | 2002 | 12055796 |
| subcellular localization of the nad+-dependent alcohol dehydrogenase in entamoeba histolytica trophozoites. | the protozoan parasite entamoeba histolytica is an ancient eukaryotic cell that shows morphologically atypical organelles and differs metabolically from higher eukaryotic cells. the aim of this study was to determine the subcellular localization of ameba nad+-dependent alcohol dehydrogenase (adh2). the enzyme activity was present in soluble and mainly in particulate material whose density was 1.105 in a sucrose gradient. by differential centrifugation, most of the adh activity sedimented at 160, ... | 2002 | 12058720 |
| lipid metabolism in mucous-dwelling amitochondriate protozoa. | entamoeba, giardia, and trichomonads are the prominent members of a group known as 'mucosal parasites'. while entamoeba and giardia trophozoites colonise the small intestine, trichomonads inhabit the genitourinary tracts of humans and animals. these protozoa lack mitochondria, well-developed golgi complexes, and other organelles typical of higher eukaryotes. nonetheless, they have developed unique metabolic pathways that allow them to survive and multiply in the small intestine and reproductive ... | 2002 | 12062485 |
| [the current status of infectious enteritis in japan--reports of the "research group for infectious enteric diseases, japan" in the last 5 years (1996-2000)]. | the patients or carriers with infectious enteritis admitted to the hospitals for infectious diseases in the last 5 years (1996-2000) were studied. the total number of cases admitted in each year were 969, 1,113, 981, 637 and 573 respectively. a total of 1,527 shigella spp. strains including 1,078 strains from overseas travelers' cases were isolated. the isolates of salmonella spp. excluding s. typhi and s. paratyphi a were 562 in number. a total of 61 vibrio cholerae o1 strains including 44 stra ... | 2002 | 12073571 |
| [ten clues to the histopathologic diagnosis of infectious skin diseases]. | specific diagnoses of infectious skin diseases can be established in dermatopathology by several methods: by algorithms based on pattern analysis, by differential diagnosis of clinicopathologically similar disorders, by attention to pitfalls, by exceptions to the rules and finally, very elegantly by clues. ten clues to specific diagnoses of infectious skin diseases which are of importance in routine practice are presented. clue 1: features of an angiocentric angiodestructive lymphoma in the cent ... | 2002 | 11974501 |
| differences between the large and small intestine in the immunodominant amoebic proteins recognized by igg and iga antibodies in balb/c mice. | we have previously reported that there are differences in the number of predominant amoebic antigens recognized by serum and small intestinal antibodies induced after local and systemic immunization with glutarldehyde-fixed entamoeba histolytica trophozoites (gft) in balb/c mice, by an immunoblot analysis. moreover, by enzyme-linked immunosorbent assay (elisa) analysis, we found differences in the antiamoebic antibody isotype patterns elicited at the large and small intestines. to further charac ... | 2002 | 11975757 |
| parasitic diarrhoea in patients with aids. | diarrhoea is a common clinical manifestation of hiv infection regardless of whether or not patients have aids. two newly recognized opportunistic coccidial protozoa are parasitic pathogens in aids patients. we attempted to determine the common parasites in indian patients with aids. | 2002 | 12044119 |
| structure and function of the entamoeba histolytica gal/galnac lectin. | gal/galnac lectin is a novel multifunctional virulence factor of the human parasite entamoeba histolytica. the native protein is a 260-kda heterodimer consisting of a type 1 membrane protein disulfide bonded to a lipid-anchored protein. each subunit has several isoforms that may form functionally different heterodimers, analogous to the integrin family of proteins. recently a second 150-kda gal/galnac lectin has been identified in e. histolytica that associates with the 260-kda lectin. the funct ... | 2002 | 12049210 |
| survey of faecal parasites in patients from western kenya. | faecal specimens were collected from patients complaining of diarrhoea and abdominal or epigastric discomfort at two hospitals. information obtained by questionnaires completed at the time of specimens collection, included demographic and clinical data. specimens were preserved in 10% formalin and polyvinyl alcohol (pva) and examined, as wet mounts and stained with wheatley's trichrome, weber, modified acid-fast and hot safranin stains. one hundred patients were examined, in 30 of them parasites ... | 2002 | 12049246 |
| detection of e. histolytica, g. lamblia and cryptosporidium copro-antigens in stool samples. | a double antibody sandwich elisa technique, using a chromatography purified antisera against e. histolytica, g. lamblia and cryptosporidium antigens, was applied to detect copro-antigens of the corrosponding parasites in 90 patients. all positive cases were diagnosed by parasitological examination and proved to have the infection solely. beside the 90 positive cases, 40 age-matched controls were included in the study, of which 20 individuals were infected with other parasites but not cryptospori ... | 2002 | 12049254 |
| effect of proteasome inhibitors on the growth, encystation, and excystation of entamoeba histolytica and entamoeba invadens. | the effect of three proteasome inhibitors, lactacystin, clasto-lactacystin beta-lactone, and mg-132, on the growth, encystation, and excystation of entamoeba histolytica and entamoeba invadens was examined. all of these drugs blocked e. histolytica growth in a concentration-dependent manner; lactacystin was most potent for the inhibition and mg-132 showed the inhibitory effect only at higher concentrations. e. invadens was more resistant to these drugs than e. histolytica. encystation of e. inva ... | 2002 | 12049464 |
| entamoeba histolytica genomic organization: identification, structure, and phylogenetic relationship of two serine-threonine protein kinases. | 2002 | 12054704 | |
| development of a diagnostic test for entamoeba histolytica using idiotype expression in human. | the protozoan parasite entamoeba histolytica is the etiological agent of human amebiasis. the pathology of the disease starts with the cytolysis of the host target cells by amoebae. it is initiated by the adhesion of trophozoites to the host cells, through surface lectin via specific receptors. these adherence lectins have been demonstrated to be highly conserved, and can be recognised by serum antibodies from patients with invasive amebiasis. some of these molecules have been used as antigens i ... | 2002 | 11983217 |
| examining entamoeba. | a conference on amebiasis and the biology of entamoeba histolytica was held in agra, india, from 11 to14 february 2002. | 2002 | 11983591 |
| a case of amebic liver abscess complicated by hemobilia due to rupture of hepatic artery aneurysm. | we report the case of a 51-year-old man with hepatic amebic abscess complicated by hepatic artery aneurysm. the patient first presented with peritonitis caused by perforating appendicitis. surgical treatment resolved peritonitis but entamoeba histolytica was detected in the colonic mucosa. subsequently, liver abscess developed and the size of the abscess increased gradually after surgery in spite of continued treatment with metronidazole. brown pus was drained from the abscess but 13 days after ... | 2002 | 11995454 |
| entamoeba histolytica genome project: an update. | 2002 | 11998693 | |
| the entamoeba histolytica ehpgp5 (mdr-like) protein induces swelling of the trophozoites and alters chloride-dependent currents in xenopus laevis oocytes. | entamoeba histolytica, the protozoan responsible for human amoebiasis, presents the multidrug resistant phenotype due to the expression of the e. histolytica p-glycoproteins ehpgpl and ehpgp5. here, we studied the protein ehpgp5 encoded by the ehpgp5 gene in emetine-sensitive trophozoites transfected with the pehneopgp5 plasmid carrying the ehpgp5 gene. the transfected trophozoites increased their drug resistance slightly, but became bigger and globular. to investigate other ehpgp5 functions fur ... | 2002 | 12002645 |
| the membrane-peripheral subunits of transhydrogenase from entamoeba histolytica are functional only when dimerized. | unlike their bacterial and mammalian counterparts, the nadp(h)- and nad(h)-binding components of proton-translocating transhydrogenase from the protozoan parasite entamoeba histolytica (denoted ehdiii and ehdi, respectively) are tethered by a polypeptide linker. the recombinant tethered fragment, ehdiii-ehdi, was prepared without its membrane-spanning dii component. dimers of ehdiii-ehdi catalyzed transhydrogenation, but monomers were inactive. the addition of ehdiii to ehdiii-ehdi monomers did ... | 2002 | 12004068 |
| differential gene expression in entamoeba histolytica isolated from amoebic liver abscess. | the majority of human infections with the intestinal protozoan parasite entamoeba histolytica remain asymptomatic. in a small proportion of infections, however, e. histolytica trophozoites penetrate the intestinal mucosa and disseminate to other organs, most commonly to the liver, where they induce abscess formation. it is believed that the ability of e. histolytica trophozoites to destroy host tissues and to survive within the liver is accomplished by a strong adaptive response, which requires ... | 2002 | 12010498 |
| fate of entamoeba histolytica during establishment of amoebic liver abscess analyzed by quantitative radioimaging and histology. | the protozoan parasite entamoeba histolytica is the causative agent of amoebiasis, a human disease characterized by dysentery and liver abscess. the physiopathology of hepatic lesions can be satisfactorily reproduced in the hamster animal model by the administration of trophozoites through the portal vein route. hamsters were infected with radioactively labeled amoebas for analysis of liver abscess establishment and progression. the radioimaging of material from parasite origin and quantificatio ... | 2002 | 12011016 |
| entamoeba histolytica lectins contain unique 6-cys or 8-cys chitin-binding domains. | the jacob lectin, the most abundant glycoprotein in the cyst wall of entamoeba invadens, contains five unique 6-cys chitin-binding domains (cbds). we identified entamoeba histolytica and entamoeba dispar genes encoding jacob homologues, each of which contains two predicted 6-cys cbds. a unique 8-cys cbd was found at the n termini of the e. histolytica chitinase and three other predicted lectins, called jessie 1 to jessie 3. the cbds of four e. histolytica lectins (jacob, chitinase, and jessies 2 ... | 2002 | 12011021 |
| characterization of a rab7-like gtpase, ehrab7: a marker for the early stages of endocytosis in entamoeba histolytica. | we have identified a 667 base pair rab7-like cdna (ehrab7) from entamoeba histolytica. the ehrab7 cdna predicts a polypeptide of at least 206 amino acids with a molecular mass of at least 24.5 kda. alignment of ehrab7 with other rab proteins demonstrated that ehrab7 shared significant homology at the amino acid level with rab7-like proteins from a number of other eukaryotes, suggesting that ehrab7 is a rab7 homolog for e. histolytica. using immunofluorescence microscopy, ehrab7 was demonstrated ... | 2002 | 12034459 |
| how protozoan parasites evade the immune response. | protozoan pathogens such as plasmodium, leishmania, trypanosoma and entamoeba are responsible for several of the most widespread and lethal human diseases. their successful survival depends mainly on evading the host immune system by, for example, penetrating and multiplying within cells, varying their surface antigens, eliminating their protein coat, and modulating the host immune response. immunosuppression is sometimes caused directly by parasite products and sometimes involves antigenic mimi ... | 2002 | 12036742 |
| amoebic versus pyogenic liver abscess. | to study the differences between the clinical presentation and complications of amoebic and pyogenic liver abscess. to correlate the diagnostic significance of entamoeba indirect haemagglutination test (e.iha) in establishing the diagnosis of amoebic liver abscess. | 2002 | 12585366 |
| segmentation and tracking of migrating cells in videomicroscopy with parametric active contours: a tool for cell-based drug testing. | this paper presents a segmentation and tracking method for quantitative analysis of cell dynamics from in vitro videomicroscopy data. the method is based on parametric active contours and includes several adaptations that address important difficulties of cellular imaging, particularly the presence of low-contrast boundary deformations known as pseudopods, and the occurence of multiple contacts between cells. first, we use an edge map based on the average intensity dispersion that takes advantag ... | 2002 | 12585703 |
| outbreak of amebiasis in tbilisi, republic of georgia, 1998. | in 1998, we investigated a suspected outbreak of amebic liver abscesses caused by entamoeba histolytica in the republic of georgia, using a case-control study. a questionnaire was administered and blood samples were obtained from cases and controls for serologic diagnosis. medical records showed that e. histolytica infections were rarely diagnosed before 1998. however, from july through september 1998, 177 cases of suspected amebiasis were identified. of 52 persons who had diagnosed liver absces ... | 2002 | 12518853 |
| defective production of reactive oxygen intermediates (roi) in a patient with recurrent amebic liver abscess. | previously, we reported the case of a man in the fourth decade of life afflicted with three independent episodes of amebic liver abscesses over a period of 4 years. previous evidence has indicated that the cellular immune response is involved in protection against recurrent invasive amebic infection, and macrophage-mediated effector mechanisms appear important for host resistance to entamoeba histolytica infection. the aim of the present work was to investigate locomotor activity and oxidative b ... | 2002 | 12518854 |
| calreticulin-like molecule in trophozoites of entamoeba histolytica hm1:imss (swissprot: accession p83003). | in this work, we report the partial sequence of a 51 kda protein of entamoeba histolytica that is highly immunogenic in humans. partial sequencing of the n-terminal end showed that 18 of the first 20 amino acid residues of the protein were identified uniquely, indicating that the final product was a homogeneous protein preparation. the n-terminal sequence that was found was: kvyfeetfengwkxiwskw. comparing the 19-amino acid sequence of the protein in automated databases shows significant similari ... | 2002 | 12518855 |
| cellular location and function of the p-glycoproteins (ehpgps) in entamoeba histolytica multidrug-resistant trophozoites. | we have studied the cellular location and the efflux pump function of the entamoeba histolytica p-glycoproteins (ehpgps) in drug-sensitive and -resistant trophozoites. polyclonal antibodies against the ehpgp384 polypeptide (375-759 amino acids) revealed a 147-kda protein by western blot. the band intensity correlated with the emetine-resistance of the trophozoites. through the confocal microscope, using the anti-ehpgp384 and fluorescein secondary antibodies, the ehpgps were found in a complex ve ... | 2002 | 12523626 |
| [distribution of entero-parasitic infections in the peruvian highland: study carried out in six rural communities of the department of puno, peru]. | a prevalence study was carried out in six rural communities in the peruvian highlands with the purpose of achieving a better understanding of the distribution of entero-parasites. the communities were located along the banks of lake titicaca, in the provinces of puno and el collao, department of puno, peru, at 3,800 m.a.s.l. to that effect, a total of 91 feces samples of adults and children from the following communities were analyzed: conchaca, puñutani, capalla, culta, maraesqueña, and jaillih ... | 2002 | 12525846 |
| mobile genetic elements in protozoan parasites. | mobile genetic elements, by virtue of their ability to move to new chromosomal locations, are considered important in shaping the evolutionary course of the genome. they are widespread in the biological kingdom. among the protozoan parasites several types of transposable elements are encountered. the largest variety is seen in the trypanosomatids-trypanosoma brucei, trypanosoma cruzi and crithidia fasciculata. they contain elements that insert site-specifically in the spliced-leader rna genes, a ... | 2002 | 12532039 |
| [the phagocytic activity of blood platelets in different parasitic diseases]. | blood platelets take part in immune reactions of the organism, especially in anti-parasitic immunity. we conducted the phagocytic activity of blood platelets. this experiments were done on 40 patients infected with giardia intestinalis (26), ascaris lumbricoides (8), entamoeba histolytica/entamoeba dispar (6). blood for analysis was collected thrice, before treatment (p1), after two weeks (p2), and after two months treatment with anti-parasitic drugs (p3). control group consisted of 32 healthy p ... | 2002 | 12632920 |
| [protozoa and food hygiene]. | 2002 | 12635345 | |
| an extracellular monoadp-ribosyl transferase activity in entamoeba histolytica trophozoites. | due to the important role of monoadp-ribosyl transferases in physiological and pathological events, we investigated whether the protozoan parasite entamoeba histolytica had monoadp-ribosyl transferase activity. reactions were initiated using ameba-free medium as the source of both enzyme and adp-ribosylation substrate(s) and [32p]nad+ as source of adp-ribose. proteins were analyzed by electrophoresis, and [32p]-labeled proteins were detected by autoradiography. using the crude extracellular medi ... | 2002 | 12503680 |
| [usefulness of multiplex-pcr for identification of entamoeba histolytica and entamoeba dispar]. | we evaluated the usefulness of a multiplex-pcr method for differentiation of entamoeba histolytica and entamoeba dispar, which are morphologically indistinguishable species. cultured trophozoites of e. histolytica hm-1: imss and e. dispar saw were used as the positive control. seven human fecal samples, from which e. histolytica-like cysts were detected by microscopic examination, and three intestinal protozoan parasites, cryptosporidium parvum hnj-1, giardia intestinalis portland-1, and blastoc ... | 2002 | 12508475 |
| [systematic search for parasites among leprosy patients in mali]. | practice of multidrug therapy in leprosy (combination dapsone + rifampicine + clofazimine) established since 1981, has significantly reduced the incidence of the disease. however, immunosuppression due to treatment of multi-drugs therapy induced adverse reactions with glucocorticoid and the change in host immune response due to the leprosy itself, might increase the risk of parasitic infections. to test this hypothesis, we carried out a case-control study at the "institut marchoux" in bamako. st ... | 2002 | 12616942 |
| pathogenic intestinal parasites and bacterial agents in solid wastes. | to determine the profile of potentially pathogenic enteric parasites and bacterial agents in municipal refuse dumps in ibadan, nigeria. | 2002 | 12630495 |
| a note on indirect hemagglutination (iha) antibody titers among hospitalized patients in thailand with amebic liver abscesses. | amebic hepatic abscess is a tropical disease with a wide spectrum of clinical presentations. a retrospective case review was performed on 39 hospitalized patients in thailand with the diagnosis of amebic liver abscess. a total of 23 men (59%) and 16 women (41%), with a mean age of 44.56 +/- 21.81 years (range, 10 to 88 years), were involved in the study. the average duration of present illness was 7.33 +/- 0.83 days. abscesses were discovered in the right lobe in 29 patients (74.4%), in the left ... | 2002 | 12466748 |
| species-specific field testing of entamoeba spp. in an area of high endemicity. | entamoeba histolytica has been separated in recent years into 2 morphologically identical species: the apathogenic e. dispar and the pathogenic e. histolytica, only the latter being pathogenic. although various laboratory techniques allow discrimination between the 2 species there is a lack of field data about the suitability of available diagnostic tests for use in epidemiological studies and few epidemiological studies using species-specific diagnosis have been performed at community level in ... | 2002 | 12474480 |
| entamoeba histolytica expressing a dominant negative n-truncated light subunit of its gal-lectin are less virulent. | the 260-kda heterodimeric gal/galnac-specific lectin (gal-lectin) of entamoeba histolytica dissociates under reducing conditions into a heavy (hgl, 170 kda) and a light subunit (lgl, 35 kda). we have previously shown that inhibition of expression of the 35-kda subunit by antisense rna causes a decrease in virulence. to further understand the role of the light subunit of the gal-lectin in pathogenesis, amoebae were transfected with plasmids encoding intact, mutated, and truncated forms of the lig ... | 2002 | 12475950 |
| prevalence of parasitic infections in a rural area of karachi, pakistan. | to find the prevalence of human intestinal pathogenic parasites. | 2002 | 12481664 |
| confronting parasites from canada. | 2002 | 12482527 | |
| entamoeba histolytica: acute granulomatous intestinal lesions in normal and neutrophil-depleted mice. | to study the role of neutrophils in the innate resistance to entamoeba histolytica intestinal infection in mice, animals were treated with anti-neutrophil monoclonal antibodies prior to intracecal parasite inoculation and the resulting lesions were compared with normal mice that had been equally infected. in contrast to our previous finding that neutrophils are critical in eliminating e. histolytica infection in the liver, we show here that neutrophils are not absolutely required to eliminate e. ... | 2002 | 12594958 |
| entamoeba histolytica: purification and characterization of ornithine decarboxylase. | ornithine decarboxylase, a rate-limiting enzyme in polyamine biosynthesis in eukaryotes, was stabilized and purified from trophozoites of the parasite protozoan e. histolytica. analytical electrophoresis revealed the presence in the purified preparations of a major polypeptide of 45 kda and barely detectable amounts of two other proteins of 70 and 120 kda. both the 45 and 70 kda polypeptides were recognized by a mouse anti-odc monoclonal antibody. the major polypeptide exhibited amino terminal s ... | 2002 | 12594962 |
| amebic infections due to the entamoeba histolytica-entamoeba dispar complex: a study of the incidence in a remote rural area of ecuador. | an epidemiologic field study was conducted in the village of borbòn in esmeraldas province in northern ecuador to compare different parasitologic methods in the diagnosis of infection with the entamoeba histolytica/entamoeba dispar complex. the results of two stool antigen detection assays (the prospect entamoeba histolytica microplate assay and the e. histolytica ii assay) were compared with isoenzyme characterization of the amebic isolates. nearly all (176 of 178, 98.9%) subjects were positive ... | 2002 | 12363056 |
| the mouse model of amebic colitis reveals mouse strain susceptibility to infection and exacerbation of disease by cd4+ t cells. | amebic colitis is an important worldwide parasitic disease for which there is not a well-established animal model. in this work we show that intracecal inoculation of entamoeba histolytica trophozoites led to established infection in 60% of c3h mice, while c57bl/6 or balb/c mice were resistant, including mice genetically deficient for il-12, ifn-gamma, or inducible no synthase. infection was a chronic and nonhealing cecitis that pathologically mirrored human disease. characterization of the infl ... | 2002 | 12370386 |
| lines and sine-like elements of the protist entamoeba histolytica. | a survey of whole genome shotgun sequences of the protozoan parasite entamoeba histolytica revealed three families of non-long terminal repeat (ltr) retrotransposons or long interspersed elements (lines) (called ehlines in this report). the 4.8 kb ehlines each had a single open reading frame with a putative nucleic acid binding motif (cchc) and restriction enzyme-like endonuclease domain located downstream of the reverse transcriptase (rt) domain. phylogenetic analysis of the rt domain placed th ... | 2002 | 12384304 |
| primary cutaneous amoebiasis: case report with review of the literature. | primary cutaneous amoebiasis is a rare clinical entity. the lesions can be successfully treated if suspected and correctly diagnosed. we report an interesting case with review of the literature. | 2002 | 12390191 |
| [what tests are needed to diagnosis amebiasis?]. | 2002 | 12395743 | |
| treatment of asymptomatic intestinal entamoeba histolytica infection. | 2002 | 12397207 | |
| amebic liver abscess. | amebic liver abscess should be suspected in travelers returning from endemic areas or in immunocompromised patients who present with fever, right upper quadrant pain, hepatomegaly, and a liver lesion on an imaging study. rapid initiation of therapy without serologic confirmation of infection, if necessary, is important to minimize complications. metronidazole is given orally or intravenously for 14 days. the drug is generally well tolerated and leads to resolution of symptoms in most patients wi ... | 2002 | 12408781 |
| remarkable genetic polymorphism among entamoeba histolytica isolates from a limited geographic area. | in order to understand genetic polymorphisms among entamoeba histolytica strains in a limited geographic area and among restricted social populations, we studied nucleotide polymorphism in dna regions that do not encode proteins (locus 1-2 and locus 5-6) and in genes coding for chitinase and for serine-rich e. histolytica protein. thirty e. histolytica isolates from domestically infected japanese amebiasis patients (male homosexuals and residents in institutions for the mentally handicapped) and ... | 2002 | 12409379 |
| potential antiamoebic property of the roots of piper longum linn. | in an earlier study we reported the effect of ethanol extracts of piper longum fruit against caecal amoebiasis in rats. a follow-up study to determine whether the roots possess similar antiamoebic activity was carried out. the ethanolic extract of roots was amoebicidal at 1,000 microg/ml in vitro and cured 88% of caecal amoebiasis cases, suggesting that both the root and fruit of p. longum possess antiamoebic activity to approximately the same extent. | 2002 | 12410557 |
| [multiple drug resistance in entamoeba histolytica]. | 2002 | 12415960 | |
| synthesis and in vitro antiprotozoal activity of 5-nitrothiophene-2-carboxaldehyde thiosemicarbazone derivatives. | several thiosemicarbazone derivatives of 5-nitrothiophene-2-carboxaldehyde were prepared by the simple process in which n(4)-thiosemicarbazone moiety was replaced by aliphatic, arylic and cyclic amine. among these thiosemicarbazones compound 11 showed significant antiamoebic activity whereas compound 3 was more active antitrichomonal than the reference drug. | 2002 | 12419387 |
| multidrug resistance in the protozoan parasite entamoeba histolytica. | in this review we discuss the mechanisms and molecules involved in the multidrug resistance (mdr) of the protozoan parasite entamoeba histolytica. drug resistant mutants exhibited the main characteristics presented by the mdr mammalian cells. they showed cross-resistance to several unrelated drugs that is reverted by calcium channel blockers. mdr phenotype in e. histolytica is regulated at a transcriptional level by the ehpgp1 gene, which is constitutively expressed and by the ehpgp5 gene, whose ... | 2002 | 12421633 |
| two ccaat/enhancer binding protein sites are cis-activator elements of the entamoeba histolytica ehpgp1 (mdr-like) gene expression. | here, we show the relevance of promoter regions (-74 to +24, -167 to -75 and -259 to -168 bp) in the transcriptional activation of the multidrug resistance gene ehpgp1 in entamoeba histolytica, using mutated plasmids and transfection assays. we also demonstrate that both ccaat/enhancer binding protein sites (-54 to -43 bp and -198 to -186 bp) are cis-activating elements of gene expression in the drug-resistant (clone c2) and -sensitive (clone a) trophozoites. nuclear proteins from trophozoites o ... | 2002 | 12427095 |
| a rho-like small gtpase of entamoeba histolytica contains an unusual amino acid residue in a conserved gdp-stabilization region and is not a substrate for c3 exoenzyme. | the entamoeba histolytica small gtp-binding protein ehrho1 has an unusual amino acid residue at a conserved site found in all known ras superfamily proteins. ehrho1 has an isoleucine at position 45, which corresponds to position 28 of human ras and rac and position 30 of human rho and cdc42. all other known small gtpases have an aromatic residue (typically phenylalanine) at this position, and mutation to a leucine renders other ras proteins constitutively active by reason of diminished affinity ... | 2002 | 12427464 |
| entamoeba histolytica: sequence conservation of the gal/galnac lectin from clinical isolates. | the gal/galnac lectin gene of entamoeba histolytica is a major amebic virulence protein responsible for interaction with host tissues. we investigated sequence differences in the gal/galnac lectin heavy subunit in three isolates from bangladesh and one isolate from georgia, each of which was determined to be genetically distinct by srehp alui digestion. interestingly, we observed only slight genetic diversity in the lectin gene as compared with the hm1:imss laboratory strain, originally a clinic ... | 2002 | 12427470 |
| endemic invasive amoebiasis in northern australia. | 2002 | 12429010 | |
| characterization of entamoeba histolytica antigens in circulating immune complexes in sera of patients with amoebiasis. | isolated circulating immune complexes (cics) from sera of patients with amoebiasis were characterized to determine entamoeba histolytica antigens that participate in the disease process. in total, 116 serum samples were collected before starting anti-amoebic therapy, and their cics were isolated by differential polyethylene glycol precipitation. the presence of amoeba-specific antigens in cics was detected by antigen capture enzyme-linked immunosorbent assay (elisa) and by immunoblot assay. anti ... | 2002 | 12430757 |
| preliminary morphological study of the effects produced by a methanolic extract of castela texana. | 2002 | 12434567 | |
| the abundant polyadenylated transcript 2 dna sequence of the pathogenic protozoan parasite entamoeba histolytica represents a nonautonomous non-long-terminal-repeat retrotransposon-like element which is absent in the closely related nonpathogenic species entamoeba dispar. | while comparing gene expression in the pathogenic organism entamoeba histolytica and the closely related but nonpathogenic species entamoeba dispar, we discovered that the e. histolytica abundant polyadenylated transcript 2 (ehapt2) and corresponding genomic copies are absent in e. dispar. although polyadenylated, ehapt2 does not contain any overt open reading frame. southern blot and sequence analyses revealed that about 500 copies of ehapt2 genomic elements were present in each cell and that t ... | 2002 | 12438355 |
| anti-inflammatory, analgesic and antiamoebic activity evaluation of pyrimido[1,6-a]benzimidazole derivatives synthesized by the reaction of ketoisothiocyanates with mono and diamines. | (un) substituted o-phenylenediamines 1a-g reacted with 3-isothiocyanatobutanal to give pyrimidobenzimidazole derivatives, 2a-g, respectively. products 4, 6 and 8, 10 were obtained by condensation of 3-isothiocyanatobutanal with 2,3-diaminopyridine, 1,4-diaminobutane and 3-isothiocyanatopropanal with 4,5-dimethyl-1,2-phenylenediamine, o-nitroaniline, respectively. s-methylation of 2f and 11b gave products 12a and 12b, respectively. anti-inflammatory and analgesic activity evaluations of 2a-g and ... | 2002 | 12446042 |
| some gastro-intestinal tract parasites in wild de brazza's monkeys (cercopithecus neglectus) in kenya. | fresh faecal droppings of wild group of de brazza's monkeys (cercopithecus neglectus), earmarked for translocation, were collected between january and july 1998. the samples were analysed using direct smears, ether-sedimentation and the harada-mori culture techniques for gastro-intestinal tract parasites (git). two species of helminths and three of protozoa were detected. entamoeba coli was found in all 40 samples screened from 11 monkeys. entamoeba histolytica was detected in 71.8% of the total ... | 2002 | 12446101 |
| the calcium-binding protein of entamoeba histolytica as a fusion partner for expression of peptides in escherichia coli. | we describe the construction of an escherichia coli expression vector, cbp that allows the c-terminal fusion of heterologous proteins to the calcium-binding protein (cabp) of the parasitic protozoan entamoeba histolytica. the intrinsic nature of this protein to remain soluble on heat treatment has been exploited in its use as a novel fusion partner. the presence of a histidine tag and an enterokinase recognition site, aid in the affinity purification and proteolytic cleavage of the fusion protei ... | 2002 | 12452805 |
| real-time pcr for detection and differentiation of entamoeba histolytica and entamoeba dispar in fecal samples. | a closed-tube, real-time pcr assay was developed for sensitive and specific detection and differentiation of the two closely related intestinal protozoan parasites entamoeba histolytica and entamoeba dispar directly from human feces. the assay is performed with the lightcycler system using fluorescence-labeled detection probes and primers amplifying a 310-bp fragment from the high-copy-number, ribosomal dna-containing ameba episome. the assay was able to detect as little as 0.1 parasite per g of ... | 2002 | 12454128 |
| evidence for lateral transfer of genes encoding ferredoxins, nitroreductases, nadh oxidase, and alcohol dehydrogenase 3 from anaerobic prokaryotes to giardia lamblia and entamoeba histolytica. | giardia lamblia and entamoeba histolytica are amitochondriate, microaerophilic protists which use fermentation enzymes like those of bacteria to survive anaerobic conditions within the intestinal lumen. genes encoding fermentation enzymes and related electron transport peptides (e.g., ferredoxins) in giardia organisms and amebae are hypothesized to be derived from either an ancient anaerobic eukaryote (amitochondriate fossil hypothesis), a mitochondrial endosymbiont (hydrogen hypothesis), or ana ... | 2002 | 12455953 |
| entamoeba histolytica: dna carrier vesicles in nuclei and kinetoplast-like organelles (ehkos). | entamoeba histolytica, the protozoan responsible for human amoebiasis, has a complex genome, whose linear chromosomes and dna circles have so far eluded detailed analysis. we report the detection by transmission electron microscopy of nuclear vesicles (0.05-0.3 microm in diameter) carrying dna in e. histolytica trophozoites. in late anaphase many of these nuclear vesicles were found to be organized in structures of approximately 2.5 x 1 microm, in association with chromosomes and microtubules. i ... | 2002 | 12172801 |
| leishmania donovani phosphofructokinase. gene characterization, biochemical properties and structure-modeling studies. | the characterization of the gene encoding leishmania donovani phosphofructokinase (pfk) and the biochemical properties of the expressed enzyme are reported. l. donovani has a single pfk gene copy per haploid genome that encodes a polypeptide with a deduced molecular mass of 53 988 and a pi of 9.26. the predicted amino acid sequence contains a c-terminal tripeptide that conforms to an established signal for glycosome targeting. l. donovani pfk showed most sequence similarity to inorganic pyrophos ... | 2002 | 12180974 |
| opportunities and obstacles in developing a vaccine for entamoeba histolytica. | the development of a vaccine against entamoeba histolytica, the causative agent of amebic colitis and liver abscess, would reduce childhood mortality in countries such as bangledesh where community-based studies have demonstrated a high prevalence of e. histolytica. immunological studies from this population have shown that protection from amebiasis is associated with mucosal anti-e. histolytica gal/galnac lectin antibodies, suggesting that a vaccine is an achievable goal. however, garnering res ... | 2002 | 12183151 |
| prevalence of intestinal parasitic pathogens in hiv-seropositive individuals in northern india. | to assess the prevalence of intestinal parasitic infections in human immunodeficiency virus (hiv)-seropositive subjects, fecal samples were collected from 120 hiv-seropositive patients and were analyzed for various intestinal parasites. thirty-six patients (30%) were found to harbor an intestinal parasite. cryptosporidium parvum was the most common (10.8%), followed by giardia lamblia (8.3%). cyclospora cayetanensis and blastocystis hominis each were detected in 3.3% of the patients, while isosp ... | 2002 | 12195048 |
| innate and acquired resistance to amebiasis in bangladeshi children. | entamoeba histolytica infection and colitis occurred in 55% and 4%, respectively, of a cohort of bangladeshi preschool children observed for 2 years. dna typing demonstrated that infecting e. histolytica isolates were genetically diverse. innate resistance to infection in children was linked to the absence of serum anti-trophozoite igg. most children who lacked serum anti-trophozoite igg failed to develop it in response to a new infection. the serum anti-trophozoite igg response clustered in fam ... | 2002 | 12195383 |
| [intestinal parasites in african asylum seekers: prevalence and risk factors]. | to determine the prevalence of potentially pathogenic intestinal parasites in asylum seekers coming from a highly endemic area, and to identify groups of asylum seekers from africa with a high risk of an intestinal parasitic infestation which is potentially harmful of their own health or that of those in their vicinity. | 2002 | 12198830 |
| epidemiology of amebiasis in a region of high incidence of amebic liver abscess in central vietnam. | the recent identification of entamoeba dispar as a separate species, which is nonpathogenic for humans but morphologically indistinguishable from entamoeba histolytica, has prompted the world health organization to recommend reinforced efforts for reassessment of the epidemiology of amebiasis and, in particular, of e. histolytica. in this regard, the distribution of amebic liver abscess (ala) cases were analyzed in the province of thua thien hué (tt hué) in central vietnam, a region known for it ... | 2002 | 12201594 |
| construction and immunogenicity of a codon-optimized entamoeba histolytica gal-lectin-based dna vaccine. | invasive amebiasis caused by entamoeba histolytica is the third leading parasitic cause of mortality, and there are no vaccines available to help control the disease. the galactose-adherence lectin (gal-lectin) is the parasite's major molecule allowing it to adhere to colonic mucin for colonization and to target cells for tissue destruction. it is immunodominant and is regarded as the most promising candidate molecule to be included in a subunit vaccine against amebiasis. in this study, we are r ... | 2002 | 12213393 |
| a patient with intestinal amoebiasis and cutaneous amoebic ulcer. | this paper reports a patient with intestinal amoebiasis in concomitant with amoebic ulcer in the thigh. the ulcer was suspected to be carcinoma, complicated cutaneous leishmaniasis or due to free-living amoebae. the diagnosis was confirmed by the pathological and parasitological demonstration of haematophagous trophozoites of e. histolytica. the patient was successfully treated with metronidazole. | 2002 | 12214920 |
| [utility of studying feces for the diagnosis and management of infants and preschool children with acute diarrhea]. | to analyze the results of a stool work-up protocol in a series of infants and preschoolers with acute diarrhea. | 2002 | 12216520 |
| differentiation of entamoeba histolytica/entamoeba dispar by pcr and their correlation with humoral and cellular immunity in individuals with clinical variants of amoebiasis. | to correlate a particular state of immunity with entamoeba spp., we used colorimetric pcr to differentiate e. histolytica from e. dispar in individuals with amoebiasis and to associate its presence with the clinical profile, including humoral and cellular immune responses to e. histolytica. our results showed high levels of antibody in acute amoebiasis and elevation of il-4 production, a cytokine related to th2 profile, associated with e. histolytica. in chronic amoebiasis, even with anti-e. his ... | 2002 | 12224582 |
| seasonal prevalence of intestinal parasites in the united states during 2000. | one-third of 5,792 fecal specimens from 2,896 patients in 48 states and the district of columbia tested positive for intestinal parasites during the year 2000. multiple infections with 2-4 parasitic species constituted 10% of 916 infected cases. blastocystis hominis infected 662 patients (23% or 72% of the 916 cases). its prevalence appears to be increasing in recent years. eighteen other species of intestinal parasites were identified. cryptosporidium parvum and entamoeba histolytica/e. dispar ... | 2002 | 12224595 |
| a monoclonal antibody to the amebic lipophosphoglycan-proteophosphoglycan antigens can prevent disease in human intestinal xenografts infected with entamoeba histolytica. | entamoeba histolytica trophozoites are covered by lipophosphoglycan-peptidoglycan molecules which may be key virulence factors. we found that pretreatment of severe combined immunodeficient mice bearing human intestinal xenografts with a monoclonal antibody to the amebic lipophosphoglycan-peptidoglycan molecules can prevent or significantly reduce the human intestinal inflammation and tissue damage that are normally seen with e. histolytica colonic infection. | 2002 | 12228321 |
| signal transduction in entamoeba histolytica induced by interaction with fibronectin: presence and activation of phosphokinase a and its possible relation to invasiveness. | interaction of entamoeba histolytica trophozoites with extracellular matrix (ecm) proteins activates signaling pathways through g-protein-coupled receptors. increments of adenylyl cyclase activity and camp produce a striking reorganization of actin into structures that apparently facilitate adhesive, locomotive, and secretory activities. the reorganization of actin is induced by phosphorylation of actin-associated proteins by diverse kinases activated during the signaling process. although camp- ... | 2002 | 12234529 |
| a cross-sectional study of intestinal parasitic infections among schoolchildren in nan province, northern thailand. | a cross-sectional study of the prevalence of intestinal parasitic infections at eight schools in bo klau district and four schools in chalerm prakiet district, nan province, in january and february, 2001. a total of 1,010 fecal samples were examined using the formalin-ether sedimentation technique. results revealed that the rate of helminthic infection was 60.0%, while protozoa accounted for 36.2% of infections; mixed infections were common, resulting in a total prevalence of both parasites of 6 ... | 2002 | 12236415 |
| fructose-1,6-bisphosphate aldolases in amitochondriate protists constitute a single protein subfamily with eubacterial relationships. | sequences of putative fructose-1,6-bisphospate aldolases (fba) in five amitochondriate unicellular eukaryotes, the diplomonads giardia intestinalis (published earlier) and spironucleus barkhanus, the pelobiont mastigamoeba balamuthi,the entamoebid entamoeba histolytica, and the parabasalid trichomonas vaginalis all belong to class ii of fbas and are highly similar to each other (>48% amino acid identity). the five protist sequences, however, do not form a monophyletic group. diplomonad fbas shar ... | 2002 | 12242011 |
| entamoeba histolytica: immunohistochemical study of hepatic amoebiasis in mouse. neutrophils and nitric oxide as possible factors of resistance. | studies in mice have not rendered conclusive data on cell and humoral factors to support the resistance of this rodent to entamoeba histolytica infection. in balb/c and c3h/hej mice inoculated with live or fixed trophozoites, we studied the evolution of the hepatic lesion, the kinetics of inflammatory cells, and the participation of some humoral factors in the development of the hepatic amoebic lesion. from the first hour, amoebae were surrounded by neutrophils containing inducible nitric oxide ... | 2002 | 12243737 |
| structure and inactivation of triosephosphate isomerase from entamoeba histolytica. | triosephosphate isomerase (tim) has been proposed as a target for drug design. tims from several parasites have a cysteine residue at the dimer interface, whose derivatization with thiol-specific reagents induces enzyme inactivation and aggregation. tims lacking this residue, such as human tim, are less affected. tim from entamoeba histolytica (ehtim) has the interface cysteine residue and presents more than ten insertions when compared with the enzyme from other pathogens. to gain further insig ... | 2002 | 12270704 |
| intestinal parasites in children with diarrhea in delhi, india. | the parasitic causes of diarrhea in children in delhi were determined by the direct smear technique; stool specimens of 127 children were examined for intestinal parasites. in 59 cases (46.5%) intestinal helminths and protozoa were demonstrated. ascaris lumbricoides was observed in 1 (0.8%) case, while trichuris trichiura was the finding in 3 (2.4%). protozoal parasites included giardia intestinalis and entamoeba histolytica in 14 (11%) cases each, balantidium coli in 3 (2.4%) cases and cryptosp ... | 2002 | 12757217 |
| gerd like symptoms as seen in a village. | twenty cases of protozoal infection presented with symptoms suggesting gerd. treatment with antacids and h2 blockers was unsuccessful in giving them relief. as they had also protozoal infection, treatment with anti-protozoal drugs gave them complete relief in the follow up period of one year after the end of treatment. hence we have named these conditions simulating peptic ulcer gerd as pseodogerd or protozoal gerd. it is suggested that the person presenting with the symptoms of gerd in our area ... | 2002 | 12645160 |
| synthesis, antiprotozoal and anticancer activity of substituted 2-trifluoromethyl- and 2-pentafluoroethylbenzimidazoles. | the synthesis of several halogenated benzimidazoles substituted in position 2 with trifluoromethyl, pentafluoroethyl and 2-thioethylaminodimethyl group is reported. antiprotozoal and anticancer activity of series of newly synthesized and previously obtained compounds was studied. all of tested bezimidazoles showed remarkable antiprotozoal activity against giardia intestinalis, entamoeba histolytica and trichomonas vaginalis. of the studied collection of halogenated benzimidazoles the most antica ... | 2002 | 12660022 |
| intestinal parasitic infections in bekasi district, west java, indonesia and a comparison of the infection rates determined by different techniques for fecal examination. | this study was undertaken to determine the current status of intestinal parasitic infections among schoolchildren in west java, indonesia, and to compare the infection rates obtained by three different methods of fecal examination. a total of 285 fecal samples were collected from 131 males and 154 females at a junior high school. samples were brought to the department of parasitology, faculty of medicine, university of indonesia, and were examined for parasites by the kato-katz thick smear metho ... | 2002 | 12693577 |
| entamoeba histolytica: rapid identification and differentiation of indian isolates by riboprinting. | entamoeba histolytica infection still remains one of the major public health problem for developing countries like india. a rapid and accurate detection of this parasite is essential for prevention and control of amoebiasis. in this study, using the method of 'riboprinting' (pcr-rflp of rrna genes from amoeba) we have analysed 15 stool samples from symptomatic patients of amoebiasis. all 15 patients of clinical amoebiasis had e. histolytica in their stool and two of the samples also showed mixed ... | 2002 | 12706746 |
| a comparative study of the intestinal parasites prevalent among children living in rural and urban settings in and around chennai. | a comparative analysis of the various intestinal parasites detected among children attending schools was carried out in a rural and urban location in and around chennai. a total of 324 stool samples were examined by routine microscopy using normal saline and lugol's iodine preparation as well as by saturated sodium chloride flotation technique. all suspicious samples were subjected to zinc sulphate concentration technique as well as modified ziehl neelson stain and trichrome stains to identify t ... | 2002 | 12718339 |
| antiamoebic chemoprophylaxis using quinfamide in children: a comparative study. | this study sought to examine whether the administration of quinfamide at 3- or 6-month intervals diminished the frequency of entamoeba histolytica cysts in stool samples compared to controls. the prospective, longitudinal, randomized, single-blind study examined children from six primary schools in celaya and neutla, guanajuato. of the 1,524 students in these schools, we selected participants for the study as follows: children were included in the study if their parents agreed in writing to the ... | 2002 | 12805964 |
| [amebic liver abscess. surgical treatment]. | 2002 | 12733337 | |
| entamoeba histolytica: intracellular distribution of the proteasome. | we have studied the intracellular distribution of proteasome subunits, corresponding to the catalytic (20s) core and the regulatory (19s) cap, in the extracellular protozoan parasite entamoeba histolytica. contrary to all cell types described to date, notably mammalian and yeast, in which the proteasome is found in the nucleus and actively imported into it, microscopic analysis and subcellular fractionation of e. histolytica trophozoites show that the proteasome is absent from the nucleus of the ... | 2002 | 12856315 |
| [evaluation of the activity of polymorphonuclear neutrophils against entamoeba histolytica antigens]. | this paper evaluates the production of superoxide as an indicator of the activity of neutrophils from healthy individuals, stimulated with entamoeaba histolytica membrane antigens, chemical stimulators and antigens from other parasites. the kinetic nitroblue tetrazolium reduction test was used. no significant differences were observed in the control group as to superoxide production by neutrophils primed with entamoeba histolytica antigens or cisticercus cellulosae, toxoplasma gondii and giardia ... | 2002 | 15849934 |
| [antiamebic effect of metronidazole proved in a study conducted in cienfuegos province]. | three studies were conducted in cienfuegos province to prove that intestinal amebiasis in cuba could be an overestimated health problem. the first two studies showed two components of this overestimation: microscopical overdiagnosis and lack of knowledge about entamoeba dispar, a non-pathogenic species, in most cases in which microscopical examination was correct. this paper reported the third study that showed the third component: the wrong belief that entamoeba histolytica is resistant to metr ... | 2002 | 15849935 |
| relationship between free radicals produced by entamoeba histolytica and its proteases complex activity. | entamoeba histolytica is a parasite which causes health problems and there has been many approaches to know what is the factor causing its pathogenicity. in the present work, we assayed if the production of free radicals by the amoeba, has a relationship with the proteases activity. when we test the dmso action (free radicals quenching activity) the specific activity of the proteases complex of the parasite were affected also. at 33.3% (v/v) concentration of dmso it was present a maximal decreas ... | 2002 | 17063776 |
| distribution and differential diagnosis of entamoeba histolytica from entamoeba dispar by the pcr-rflp method in central iran. | entamoeba histolytica and entamoeba dispar are two morphologically indistinguishable human protozoan parasites that are genetically distinct species. the potential invasive pathogenic entamoeba histolytica and non-invasive parasite entamoeba dispar can be differentiated by molecular and other methods. we used polymerase chain reaction (pcr) to determine the ratio of the two species in a population in tehran and karaj in central iran. | 2003 | 16868369 |