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accurate inference of shoot biomass from high-throughput images of cereal plants.with the establishment of advanced technology facilities for high throughput plant phenotyping, the problem of estimating plant biomass of individual plants from their two dimensional images is becoming increasingly important. the approach predominantly cited in literature is to estimate the biomass of a plant as a linear function of the projected shoot area of plants in the images. however, the estimation error from this model, which is solely a function of projected shoot area, is large, prohi ...201121284859
mapping qtl for grain yield, yield components, and spike features in a doubled haploid population of bread wheat.a doubled haploid (dh) population derived from a cross between the japanese cultivar 'fukuho-kumogi' and the israeli wheat line 'oligoculm' was used to map genome regions involved in the expression of grain yield, yield components, and spike features in wheat (triticum aestivum l). a total of 371 markers (rapd, ssr, rflp, aflp, and two morphological traits) were used to construct the linkage map that covered 4190 cm of wheat genome including 28 linkage groups. the results of composite interval m ...201121635161
a proteomics view on the role of drought-induced senescence and oxidative stress defense in enhanced stem reserves remobilization in wheat.drought is one of the major factors limiting the yield of wheat (triticum aestivum l.) particularly during grain filling. under terminal drought condition, remobilization of pre-stored carbohydrates in wheat stem to grain has a major contribution in yield. to determine the molecular mechanism of stem reserve utilization under drought condition, we compared stem proteome patterns of two contrasting wheat landraces (n49 and n14) under a progressive post-anthesis drought stress, during which period ...201121621021
spectroscopic analysis of diversity of arabinoxylan structures in endosperm cell walls of wheat cultivars (triticum aestivum) in the healthgrain diversity collection.fifty bread wheat (triticum aestivum l.) cultivars were selected from the healthgrain germplasm collection based on variation in their contents of total and water-extractable arabinoxylan. ft-ir spectroscopic mapping of thin transverse sections of grain showed variation in cell wall arabinoxylan composition between the cultivars, from consisting almost entirely of low-substituted arabinoxylan (e.g., t.aestivum 'claire') to almost entirely of highly substituted arabinoxylan (e.g., t.aestivum 'man ...201121615152
phosphate utilization efficiency correlates with expression of low-affinity phosphate transporters and noncoding rna, ips1, in barley.genetic variation in phosphorus (p) efficiency exists among wheat (triticum aestivum) and barley (hordeum vulgare) genotypes, but the underlying mechanisms for the variation remain elusive. high- and low-affinity phosphate (pi) pht1 transporters play an indispensable role in p acquisition and remobilization. however, little is known about genetic variation in pht1 gene expression and association with p acquisition efficiency (pae) and p utilization efficiency (pue). here, we present quantitative ...201121606317
allelic variation, alternative splicing and expression analysis of psy1 gene in hordeum chilense roem. et schult.the wild barley hordeum chilense roem. et schult. is a valuable source of genes for increasing carotenoid content in wheat. tritordeums, the amphiploids derived from durum or common wheat and h. chilense, systematically show higher values of yellow pigment colour and carotenoid content than durum wheat. phytoene synthase 1 gene (psy1) is considered a key step limiting the carotenoid biosynthesis, and the correlation of psy1 transcripts accumulation and endosperm carotenoid content has been demon ...201121603624
enrichment of a common wheat genetic map and qtl mapping for fatty acid content in grain.dart and ssr markers were used to saturate and improve a previous genetic map of rils derived from the cross chuan35050 × shannong483. the new map comprised 719 loci, 561 of which were located on specific chromosomes, giving a total map length of 4008.4 cm; the rest 158 loci were mapped to the most likely intervals. the average chromosome length was 190.9 cm and the marker density was 7.15 cm per marker interval. among the 719 loci, the majority of marker loci were darts (361); the rest included ...201121600399
effect of four types of dietary fiber on the technological quality of pasta.the development of dietary fiber-enriched foods permits to obtain products with functional properties but can cause several problems in technological quality. the aim of this study was to study the quality of pasta obtained by replacing bread wheat flour with resistant starch ii (rsii), resistant starch iv (rsiv), oat bran (ob) and inulin (in) with the purpose of improving their nutritional quality. rsii, rsiv, ob and in were substituted for a portion of bread wheat flour at levels 2.5%, 5.0%, 7 ...201121593287
insights of interaction between small and large subunits of adp-glucose pyrophosphorylase from bread wheat (triticum aestivum l.).lack of knowledge of three dimensional structures of small and large subunits of adp- glucose pyrophosphorylase (agpase) in wheat has hindered efforts to understand the binding specifities of substrate and catalytic mechanism. thus, to understand the structure activity relationship, 3d structures were built by homology modelling based on crystal structure of potato tuber adp-glucose pyrophosphorylase. selected models were refined by energy minimization and further validated by procheck and prosa ...201121572880
investigation of genetic diversity and population structure of common wheat cultivars in northern china using dart markers.in order to help establish heterotic groups of chinese northern wheat cultivars (lines), diversity arrays technology (dart) markers were used to investigate the genetic diversity and population structure of chinese common wheat (triticum aestivum l.).201121569312
comparative analysis of the n-genome in diploid and polyploid aegilops species.the genetic classification for the n-genome chromosomes has been developed on the basis of c-banding analysis on the set of triticum aestivum × aegilops uniaristata single chromosome addition lines and examination of a. uniaristata (2n = 2 × = 14, nn), aegilops ventricosa (2n = 4 × = 28, ddnn) and aegilops recta (2n = 6 × = 42, uux(n)x(n)nn) accessions carrying intergenomic translocations using fluorescence in situ hybridisation with probes for three repetitive dna sequences as well as the 5s an ...201121556954
[antipodal complex development in the embryo sac of wheat].dynamics of an antipodal complex formation in wheat (triticum aestivum l.) has been observed in detail using a reconstruction of serial semifine sections. three consecutive crucial stages have been identified in the development of the antipodal complex: (1) proliferation of initial cells, (2) growth and functional differentiation of antipodal cells, and (3) cell apoptosis. specific features of the mitotic division of antipodal cells have been characterized. it has been shown that the structure o ...201121542339
acquisition and assimilation of nitrogen as peptide-bound and d-enantiomers of amino acids by wheat.nitrogen is a key regulator of primary productivity in many terrestrial ecosystems. historically, only inorganic n (nh(4)(+) and no(3)(-)) and l-amino acids have been considered to be important to the n nutrition of terrestrial plants. however, amino acids are also present in soil as small peptides and in d-enantiomeric form. we compared the uptake and assimilation of n as free amino acid and short homopeptide in both l- and d-enantiomeric forms. sterile roots of wheat (triticum aestivum l.) pla ...201121541281
calcium and magnesium enhance arsenate rhizotoxicity and uptake in triticum aestivum.cations such as calcium (ca) and magnesium (mg) alleviate toxicities of cationic toxicants and increase those of anionic toxicants such as arsenic (as) present as arsenate under aerobic conditions. increasing evidence exists that these phenomena are related to the outer surface electrical potential (ψ 0°) of the root cell plasma membrane (pm). short-term (48-h) nutrient culture experiments with wheat (triticum aestivum l.) seedlings investigated ca and mg effects on arsenate rhizotoxicity and up ...201121538485
cadmium pollution enhanced ozone damage to winter wheat: biochemical and physiological evidences.combined effects of ozone (03) and cadmium (cd) on growth and physiology of winter wheat (triticum aestivum l. cv. jm22) were determined. wheat plants were grown without or with cd and exposed to charcoal-filtered air (< 10 ppb o3) or elevated o3 (80 +/- 5 ppb, 7 hr/day) for 20 days. results showed that 03 considerably depressed light saturated net photosynthetic rate (-20%), stomatal conductance (-33%), chlorophyll content (-33%), and total biomass (-29%) without cd. the corresponding decreases ...201121516999
molecular characterization and genomic mapping of the pathogenesis-related protein 1 (pr-1) gene family in hexaploid wheat (triticum aestivum l.).the group 1 pathogenesis-related (pr-1) proteins, known as hallmarks of defense pathways, are encoded by multigene families in plants as evidenced by the presence of 22 and 32 pr-1 genes in the finished arabidopsis and rice genomes, respectively. here, we report the initial characterization and mapping of 23 pr-1-like (tapr-1) genes in hexaploid wheat (triticum aestivum l.), which possesses one of the largest (>16,000 megabases) genomes among monocot crop plants. sequence analysis revealed that ...201121516334
extensive and heritable epigenetic remodeling and genetic stability accompany allohexaploidization of wheat.allopolyploidy has played a prominent role in organismal evolution, particularly in angiosperms. allohexaploidization is a critical step leading to the formation of common wheat as a new species, triticum aestivum, as well as for bestowing its remarkable adaptability. a recent study documented that the initial stages of wheat allohexaploidization was associated with rampant genetic and epigenetic instabilities at genomic regions flanking a retrotransposon family named veju. although this finding ...201121515577
detection of sugar accumulation and expression levels of correlative key enzymes in winter wheat (triticum aestivum) at low temperatures.carbohydrate accumulation is common in frost-resistant plants, and many enzymes participate in this process. the sugar content and expression levels of metabolic enzymes related to sugar biosynthesis in response to drops in temperature were measured in two cultivars of winter wheat (triticum aestivum) with different cold tolerances. the results indicate that the two cultivars examined, dongnongdongmai 1 and jimai 22, accumulated high levels of carbohydrate before november 4 (above 0°c), and that ...201121512254
synthesis and biological activity of n-acyl o-indolylalkyl ethanolamines.the plant-growth regulators, indole-3-carboxylic acids, were introduced into n-acyl ethanolamines, and a series of n-acyl o-indolylalkyl ethanolamines were prepared. their biological activities to regulate rape hypocotyl elongation, cucumber cotyledon expansion and common wheat coleoptile growth were tested. the results indicate that the title compounds inhibited rape hypocotyl elongation, especially the indole-3-propionic acid derivatives, whose bioactivity was better than that of indole-3-acet ...201121512235
effect of organic and conventional crop rotation, fertilization, and crop protection practices on metal contents in wheat (triticum aestivum).the effects of organic versus conventional crop management practices (crop rotation, crop protection, and fertility management strategies) on wheat yields and grain metal (al, cd, cu, ni, pb, and zn) concentrations were investigated in a long-term field trial. the interactions between crop management practices and the season that the crop was grown were investigated using univariate and redundancy analysis approaches. grain yields were highest where conventional fertility management and crop pro ...201121495704
phylogenetic analysis of c, m, n, and u genomes and their relationships with triticum and other related genomes as revealed by lmw-gs genes at glu-3 loci.phylogenetic relationships between the c, u, n, and m genomes of aegilops species and the genomes of common wheat and other related species were investigated by using three types of low-molecular-weight glutenin subunit (lmw-gs) genes at glu-3 loci. a total of 20 lmw-gs genes from aegilops and triticum species were isolated, including 11 lmw-m type and 9 lmw-i type genes. particularly, four lmw-m type and three lmw-i type subunits encoded by the genes on the c, n, and u genomes possessed an extr ...201121491971
molecular analysis of common wheat genes encoding three types of cytosolic heat shock protein 90 (hsp90): functional involvement of cytosolic hsp90s in the control of wheat seedling growth and disease resistance.heat shock protein 90 (hsp90) molecular chaperones play important roles in plant growth and responses to environmental stimuli. however, little is known about the genes encoding hsp90s in common wheat. here, we report genetic and functional analysis of the genes specifying cytosolic hsp90s in this species. three groups of homoeologous genes (tahsp90.1, tahsp90.2 and tahsp90.3), encoding three types of cytosolic hsp90, were isolated. the loci containing tahsp90.1, tahsp90.2 and tahsp90.3 genes we ...201121488877
adverse effects of chromium oxide nano-particles on seed germination and growth in triticum aestivum l.in this study, seeds of triticum aestivum l. (poaceae) were exposed to 0-100 microg/ml chromium oxide nanoparticles (cr2o3, nps) to study the phytotoxic effects on seed germination and seedling growth. it has been observed that 25-100 microg/ml cr2o3, nps inhibited the seed germination and seedling growth in concentration dependent manner. the present study suggests that release of cr2o3, nps in environment may adversely affect the wheat production.201121485872
advanced backcross qtl mapping of resistance to fusarium head blight and plant morphological traits in a triticum macha × t. aestivum population.while many reports on genetic analysis of fusarium head blight (fhb) resistance in bread wheat have been published during the past decade, only limited information is available on fhb resistance derived from wheat relatives. in this contribution, we report on the genetic analysis of fhb resistance derived from triticum macha (georgian spelt wheat). as the origin of t. macha is in the caucasian region, it is supposed that its fhb resistance differs from other well-investigated resistance sources. ...201121479934
assessment of allergenicity of diploid and hexaploid wheat genotypes: identification of allergens in the albumin/globulin fraction.wheat is an important part of the daily diet of millions of people. however, this staple food is also responsible for food allergies. ancient cultivars of wheat are gaining interest today but nothing is known about their allergenicity. many wheat proteins have been reported as causative food allergens, including some prolamin-type gluten proteins, and salt soluble proteins of the albumin/globulin (a/g) type. the objective of this work is to obtain information about the allergenicity of the salt ...201121473946
studies on immobilization and partial characterization of lipases from wheat seeds (triticum aestivum).the objective of this study was to provide some features on immobilization and partial characterization of lipases from wheat seeds. the optimum ph and temperature were found to be 5.5 and 32-37 °c, respectively. the stability of the concentrated enzymatic extract to high temperatures (25, 35, 45, and 55 °c) showed that the incubation of the extract at 55 °c led to its complete inactivation. the concentrated enzymatic extract kept 90% of its hydrolytic and esterification activities until 70 and ...201121468634
wheat hybridization and polyploidization results in deregulation of small rnas.speciation via interspecific or intergeneric hybridization and polyploidization triggers genomic responses involving genetic and epigenetic alterations. such modifications may be induced by small rnas, which affect key cellular processes, including gene expression, chromatin structure, cytosine methylation and transposable element (te) activity. to date, the role of small rnas in the context of wide hybridization and polyploidization has received little attention. in this work, we performed high ...201121467573
overexpression of tanac69 leads to enhanced transcript levels of stress up-regulated genes and dehydration tolerance in bread wheat.nac proteins are plant-specific transcription factors and enriched with members involved in plant response to drought stress. in this study, we analyzed the expression profiles of tanac69 in bread wheat using affymetrix wheat genome array datasets and quantitative rt-pcr. tanac69 expression was positively associated with wheat responses to both abiotic and biotic stresses and was closely correlated with a number of stress up-regulated genes. the functional analyses of tanac69 in transgenic wheat ...201121459832
tasrg, a wheat transcription factor, significantly affects salt tolerance in transgenic rice and arabidopsis.based on our microarray-analysis results of the salt tolerant wheat mutant, rh8706-49, an unknown salt-induced gene containing a conserved duf662 domain was identified and cloned; we named this gene tasrg (triticum aestivum salt response gene). real-time quantitative pcr analyses showed that the expression of this gene was affected by salt, drought, cold, aba and other stress conditions. the overexpression of tasrg in wild-type arabidopsis resulted in increased salt tolerance compared with wild- ...201121457711
members of the rkd transcription factor family induce an egg cell-like gene expression program.in contrast to animals, the life cycle of higher plants alternates between a gamete-producing (gametophyte) and a spore-producing (sporophyte) generation. the female gametophyte of angiosperms consists of four distinct cell types, including two gametes, the egg and the central cell, which give rise to embryo and endosperm, respectively. based on a combined subtractive hybridization and virtual subtraction approach in wheat (triticum aestivum l.), we have isolated a class of transcription factors ...201121457369
puroindoline-a, a lipid binding protein from common wheat, spontaneously forms prolate protein micelles in solution.the self-assembly in solution of puroindoline-a (pin-a), an amphiphilic lipid binding protein from common wheat, was investigated by small angle neutron scattering, dynamic light scattering and size exclusion chromatography. pin-a was found to form monodisperse prolate ellipsoidal micelles with a major axial radius of 112 ± 4.5 å and minor axial radius of 40.4 ± 0.18 å. these protein micelles were formed by the spontaneous self-assembly of 38 pin-a molecules in solution and were stable over a wi ...201121451840
conservation tillage, optimal water and organic nutrient supply enhance soil microbial activities during wheat (triticum aestivum l.) cultivation.the field experiments were conducted on sandy loam soil at new delhi, during 2007 and 2008 to investigate the effect of conservation tillage, irrigation regimes (sub-optimal, optimal and supra-optimal water regimes), and integrated nutrient management (inm) practices on soil biological parameters in wheat cultivation. the conservation tillage soils has shown significant (p<0.05) increase in soil respiration (81.1%), soil microbial biomass carbon (smbc) (104%) and soil dehydrogenase (dh) (59.2%) ...201124031665
[characteristics of common wheat cultivars of west siberia carrying the wheat-rye 1rs.1bl translocation].using genomic in situ hybridization, among the common wheat cultivars produced in west siberia (siberian research institute of agriculture, omsk) with the involvement of the winter wheat cultivar kavkaz carrying the wheat-rye 1rs.1bl translocation we identified three cultivars with this translocation: omskaya 29, omskaya 37, and omskaya 38. the protein and crude gluten contents in the grain of these cultivars are equal to or exceed the levels observed in cultivars without the wheat-rye transloca ...201121446180
quality of bread made from ozonated wheat (triticum aestivum l.) flour.ozone gas could be used as a fumigant during grain and flour storage. experiments were conducted to determine the effects of exposure to ozone and the effects of blending ozone-treated flour with control flour on flour functionality and bread-making quality.201121445841
susceptibility of tribolium castaneum (coleoptera: tenebrionidae) life stages to flameless catalytic infrared radiation.the susceptibility of various life stages of the red flour beetle, tribolium castaneum (herbst) (coleoptera: tenebrionidae), a pest of stored wheat, triticum aestivum l., to flameless catalytic infrared radiation in the 3-7-microm range was evaluated in the laboratory. immature stages were collected from flour infested with t. castaneum adults only for 1 d. stages collected after 1 d represented eggs (collected on day 0); those collected after 7, 14, and 21 d from day 0 represented larvae in dif ...201121404874
wheat grain development is characterized by remarkable trehalose 6-phosphate accumulation pregrain filling: tissue distribution and relationship to snf1-related protein kinase1 activity.trehalose 6-phosphate (t6p) is a sugar signal that regulates metabolism, growth, and development and inhibits the central regulatory snf1-related protein kinase1 (snrk1; akin10/akin11). to better understand the mechanism in wheat (triticum aestivum) grain, we analyze t6p content and snrk1 activities. t6p levels changed 178-fold 1 to 45 d after anthesis (daa), correlating with sucrose content. t6p ranged from 78 nmol g(-1) fresh weight (fw) pregrain filling, around 100-fold higher than previously ...201121402798
starch waxiness in hexaploid wheat (triticum aestivum l.) by nir reflectance spectroscopy.wheat (triticum aestivum l.) breeding programs are currently developing varieties that are free of amylose (waxy wheat), as well as genetically intermediate (partial waxy) types. successful introduction of waxy wheat varieties into commerce is predicated on a rapid methodology at the commodity point of sale that can test for the waxy condition. near-infrared (nir) reflectance spectroscopy, one such technology, was applied to a diverse set of hard winter (hexaploid) wheat breeders' lines represen ...201121401107
technological, rheological and yield differences among egyptian wheat varieties.a field experiment was conducted during the two winter seasons of 2007/2008 and 2008/2009 to evaluate the yield and its components, milling, technological and rheological characteristics as well as the correlation between these characteristics of eight bread wheat (triticum aestivum l.) cultivars (gemmeiza 7, gemmeiza 9, gemmeiza 10, sakha 92, sakha 93, sakha 94, giza 164 and giza 168). these cultivars were chosen because they were new.201121384350
microbial activity and water-soluble trace element species in the rhizosphere of spring wheat (triticum aestivum cv. usu-perigee).the influence of microbial activity on the concentration and speciation of trace elements (tes) was assessed in a study on the bioavailability of tes for edible plants. a growth chamber experiment with spring wheat (triticum aestivum cv. usu-perigee) was conducted and the bulk (bk) and the rhizosphere (rz) soil components were collected at maturity. a characterization of the microbial activity and population was made by measuring the microbial biomass, enzymes (acid phosphatase, arylsulfatase, d ...201121359293
major genes for na+ exclusion, nax1 and nax2 (wheat hkt1;4 and hkt1;5), decrease na+ accumulation in bread wheat leaves under saline and waterlogged conditions.two major genes for na(+) exclusion in durum wheat, nax1 and nax2, that were previously identified as the na(+) transporters tmhkt1;4-a2 and tmhkt1;5-a, were transferred into bread wheat in order to increase its capacity to restrict the accumulation of na(+) in leaves. the genes were crossed from tetraploid durum wheat (triticum turgidum ssp. durum) into hexaploid bread wheat (triticum aestivum) by interspecific crossing and marker-assisted selection for hexaploid plants containing one or both g ...201121357768
synthesizing double haploid hexaploid wheat populations based on a spontaneous alloploidization process.doubled haploid (dh) populations are useful to scientists and breeders in both crop improvement and basic research. current methods of producing dhs usually need in vitro culture for extracting haploids and chemical treatment for chromosome doubling. this report describes a simple method for synthesizing dhs (syndh) especially for allopolyploid species by utilizing meiotic restitution genes. the method involves three steps: hybridization to induce recombination, interspecific hybridization to ex ...201121356528
characterisation of a new stripe rust resistance gene yr47 and its genetic association with the leaf rust resistance gene lr52.two iranian common wheat landraces aus28183 and aus28187 from the watkins collection showed high levels of seedling resistance against australian pathotypes of leaf rust and stripe rust pathogens. chi-squared analyses of rust response segregation among f(3) populations derived from crosses of aus28183 and aus28187 with a susceptible genotype aus27229 revealed monogenic inheritance of leaf rust and stripe rust resistance. as both genotypes produced similar leaf rust and stripe rust infection type ...201121344185
development of a new marker system for identifying the complex members of the low-molecular-weight glutenin subunit gene family in bread wheat (triticum aestivum l.).low-molecular-weight glutenin subunits (lmw-gss) play an important role in determining the bread-making quality of bread wheat. however, lmw-gss display high polymorphic protein complexes encoded by multiple genes, and elucidating the complex lmw-gs gene family in bread wheat remains challenging. in the present study, using conventional polymerase chain reaction (pcr) with conserved primers and high-resolution capillary electrophoresis, we developed a new molecular marker system for identifying ...201121344181
stable chloroplast transformation of immature scutella and inflorescences in wheat (triticum aestivum l.).chloroplast transformation in wheat was achieved by bombardment of scutella from immature embryos and immature inflorescences, respectively. a wheat chloroplast site-specific expression vector, pbagnrk, was constructed by placing an expression cassette containing neomycin phosphotransferase ii (nptii) and green fluorescent protein (gfp) as selection and reporter genes, respectively, in the intergenic spacer between atpb and rbcl of wheat chloroplast genome. integration of gfp gene in the plastom ...201121343162
assessing the role of root plasma membrane and tonoplast na+/h+ exchangers in salinity tolerance in wheat: in planta quantification methods.this work investigates the role of cytosolic na+ exclusion in roots as a means of salinity tolerance in wheat, and offers in planta methods for the functional assessment of major transporters contributing to this trait. an electrophysiological protocol was developed to quantify the activity of plasma membrane na+ efflux systems in roots, using the microelectrode ion flux estimation (mife) technique. we show that active efflux of na+ from wheat root epidermal cells is mediated by a sos1-like homo ...201121342209
[preferential elimination of chromosome 5r of rye in the progeny of 5r5d dimonosomics].transmission of chromosome 5r of rye (secale cereale l.) and chromosome 5d of common wheat (triticum aestivum l.) through gametes of 5r5d dimonosomics (2n = 42, 20w" + 5r' + 5d') was studied. chromosome 5r was found to have lower competitiveness as compared to 5d. gametes with the rye chromosome were two times less often involved in the formation of a progeny. the combined frequency of the karyotypes of wheat (5d5d) and wheat monosomics (5d) was 11.6-fold higher than the frequency of the karyoty ...201121954615
identification of a large deletion in the first intron of the vrn-d1 locus, associated with loss of vernalization requirement in wild wheat progenitor aegilops tauschii coss.vernalization promotes flowering in winter wheat cultivars, whereas spring wheat cultivars are able to transition from vegetative to reproductive phase without vernalization. the wheat vernalization requirement is mainly controlled by the major locus vrn-1, an apetala1/fruitfull mads-box gene homolog. to study natural variation of the vernalization requirement in a wild progenitor of common wheat, we sequenced the vrn-d(t)1 locus in four accessions of aegilops tauschii coss. some structural muta ...201121952208
reference genes for gene expression studies in wheat flag leaves grown under different farming conditions.internal control genes with highly uniform expression throughout the experimental conditions are required for accurate gene expression analysis as no universal reference genes exists. in this study, the expression stability of 24 candidate genes from triticum aestivum cv. cubus flag leaves grown under organic and conventional farming systems was evaluated in two locations in order to select suitable genes that can be used for normalization of real-time quantitative reverse-transcription pcr (rt- ...201121951810
[the chiral mutagens: cytogenetic effects on higher plants].the paper covers investigation of cytogenetic activity of chiral mutagens and their specific effects on the plant cells chromosomes of soft winter wheat (triticum aestivum l.). comparative analysis of cytogenetic activity of chiral neu: s(+)1-n-nitroso- 1-n-methyl-3-n-sec-buthylureas (s(+)nmsbu) and r(-)1-n-nitroso- 1n-methyl-3-nsec-buthylureas (r(-)nmsbu) on winter wheat was performed. as it was shown by the frequency of chromosomal aberrations the s(+) stereoisomer was twice more active than r ...201121950141
[analysis of diversity of russian and ukrainian bread wheat (triticum aestivum l.) cultivars for high-molecular-weight glutenin subunits].the allelic diversity of high-moleculat-weght glutenin subunits (h wigs) in russian and ukrainian bread wheat cultivars was analyzed. the diversity of spring wheat cultivars for alleles of the glu-1 loci is characterized by medium values of the polymorphism index (polymorphism information content, plc), and in winter wheats it varies from high at the glu-a1 locus to low at the glu-d1 locus. the spring and winter cultivars differ significantly in the frequencies of alleles of the glutenin loci. t ...201121938954
down-regulating γ-gliadins in bread wheat leads to non-specific increases in other gluten proteins and has no major effect on dough gluten strength.gliadins are a major component of gluten proteins but their role in the mixing of dough is not well understood because their contribution to wheat flour functional properties are not as clear as for the glutenin fraction.201121935456
heat damage of water biscuits from einkorn, durum and bread wheat flours.to limit heat damage and improve the nutritional properties of bakery products, furosine, glucosylisomaltol, hydroxymethylfurfural, furfural, sugars, α-amylase, β-amylase and colour were assessed during the production of water biscuits from three einkorn, three bread and one durum wheat flours. heat damage indices, colour and aw development during baking (from 25 to 75min duration) of water biscuits from one bread wheat were also studied. furosine was more abundant in durum (86.0±6.29mg/100g pro ...201125212158
efficacy of heat treatment for disinfestation of concrete grain silos.field experiments were conducted in 2007 and 2008 to evaluate heat treatment for disinfestations of empty concrete elevator silos. a mobile heat treatment unit was used to introduce heat into silos to attain target conditions of 50 degrees c for at least 6 h. ventilated plastic containers with a capacity of 100 g of wheat, triticum aestivum l., held rhyzopertha dominica (f.) (coleoptera: bostrichidae) and tribolium castaneum (herbst) (coleoptera: tenebrionidae). polyvinyl chloride containers wit ...201121882711
combined effect of iron and zinc on micronutrient levels in wheat (triticum aestivum l.).a nutrient solution experiment was conducted to investigate the effect of fe and zn supply on fe, zn, cu, and mn concentrations in wheat plants. the experiment used a factorial combination of two fe levels (0 and 5 mg l(-1)) and three zn levels (0, 0.1 and 10 mg i(-1)). the supply of fe (5 mg l(-1)) and zn (0.1 mg l(-1)) increased plant dry weight and leaf chlorophyll content compared to the fe or zn deficient (0 mg 11) treatments. however, excess zn supply (10 mg l(-1)) reduced plant dry weight ...201121882661
dispersed benzoxazinone gene cluster: molecular characterization and chromosomal localization of glucosyltransferase and glucosidase genes in wheat and rye.benzoxazinones (bxs) are major defensive secondary metabolites in wheat (triticum aestivum), rye (secale cereale), and maize (zea mays). here, we identified full sets of homeologous and paralogous genes encoding bx glucosyltransferase (gt) and bx-glucoside glucosidase (glu) in hexaploid wheat (2n = 6x = 42; aabbdd). four gt loci (tagta-tagtd) were mapped on chromosomes 7a, 7b (two loci), and 7d, whereas four glu1 loci (taglu1a-taglu1d) were on chromosomes 2a, 2b (two loci), and 2d. transcript le ...201121875895
intergenic spacer length variants in old portuguese bread wheat cultivars.the intergenic spacer of the ribosomal dna is highly variable, but is location specific in the nucleolar organizer region of the chromosomes. this study provides an event of high level of polymorphism / size variation and occurrence of 14 unique phenotypes in 48 landraces of portuguese bread wheat cultivars for igs-amplified products obtained by pcr-rflp technique performed with taqi. the attendant igs polymorphism has been used to deduce affinities between landraces. some of the high molecular ...201121869468
[morphological and genetic characteristics of leaf hairiness in wheat (triticum aestivum l.) as analyzed by computer-aided phenotyping].computer-aided image processing was used to study the morphology of leaf hairiness in the wheat cultivars saratovskaya 29 and golubka, as well as the introgressed strain 102/00i of the cultivar rodina carrying the hairiness control gene introgressed from aegilops speltoides. morphological differences in leaf hairiness were detected and described in detail. the genetic control ofhairiness was studied in two cultivars (golubka and saratovskaya 29) with similar hairiness patterns. crossing these cu ...201121866865
pcr-based isolation and identification of full-length low-molecular-weight glutenin subunit genes in bread wheat (triticum aestivum l.).low-molecular-weight glutenin subunits (lmw-gss) are encoded by a multi-gene family and are essential for determining the quality of wheat flour products, such as bread and noodles. however, the exact role or contribution of individual lmw-gs genes to wheat quality remains unclear. this is, at least in part, due to the difficulty in characterizing complete sequences of all lmw-gs gene family members in bread wheat. to identify full-length lmw-gs genes, a polymerase chain reaction (pcr)-based met ...201121830110
intake and digestion of whole-crop barley and wheat silages by dairy heifers.the effect of maturity at harvest on the digestibility and intake of large bale silage made from whole-crop barley and wheat when fed to growing heifers was evaluated. two crops of spring barley (hordeum distichum cv. filippa and kinnan) and 1 of winter wheat (triticum aestivum cv. olevin) were harvested at the heading, milk, and dough stages of maturity. the silage was fed to 36 dairy heifers in a balanced crossover experiment with 3 periods and 9 treatments (diets based on 3 crops and 3 stages ...201121821807
differential effects of a post-anthesis fertilizer regimen on the wheat flour proteome determined by quantitative 2-de.mineral nutrition during wheat grain development has large effects on wheat flour protein content and composition, which in turn affect flour quality and immunogenic potential for a commodity of great economic value. however, it has been difficult to define the precise effects of mineral nutrition on protein composition because of the complexity of the wheat flour proteome. recent improvements in the identification of flour proteins by tandem mass spectrometry (ms/ms) and the availability of a c ...201121816081
redox changes during cold acclimation affect freezing tolerance but not the vegetative/reproductive transition of the shoot apex in wheat.cold acclimation is necessary for winter wheat (triticum aestivum l.) to achieve its genetically determined maximum freezing tolerance, and cold also fulfils the vernalisation requirement. chromosome 5a is a major regulator of these traits. the aim of the present study was to discover whether changes in the half-cell redox potential of the glutathione/glutathione disulphide (gsh/gssg) and ascorbate/dehydroascorbate (aa/dha) couples induced by cold acclimation are related to freezing tolerance an ...201121815980
identification of changes in wheat (triticum aestivum l.) seeds proteome in response to anti-trx s gene.thioredoxin h (trx h) is closely related to germination of cereal seeds. the cdna sequences of the thioredoxin s (trx s) gene from phalaris coerulescens and the thioredoxin h (trx h) gene from wheat are highly homologous, and their expression products have similar biological functions. transgenic wheat had been formed after the antisense trx s was transferred into wheat, and it had been certified that the expression of trx h decreased in transgenic wheat, and transgenic wheat has high resistance ...201121811579
chromosomal and genome-wide molecular changes associated with initial stages of allohexaploidization in wheat can be transit and incidental.genomic instability can be induced by nascent allopolyploidization in plants. however, most previous studies have not defined to what extent the allopolyploidy-induced rapid genomic instability represents a general response, and hence important to evolution, or merely incidental events occurring stochastically in a limited number of individuals. we report here that in a newly formed allohexaploid wheat line between tetraploid wheat triticum turgidum subsp. durum (genome bbaa) and aegilops tausch ...201121797821
factors responsible for deep-sowing tolerance in wheat seedlings: varietal differences in cell proliferation and the co-ordinated synchronization of epidermal cell expansion and cortical cell division for the gibberellin-mediated elongation of first internodes.a wheat cultivar, triticum aestivum 'hong mang mai', shows tolerance to deep-sowing conditions by extreme elongation of the first internode, likely mediated by the gibberellin (ga) response. to understand factors involved in the response of this deep-sowing-tolerant cultivar, cell expansion and division that confer elongation on the first internodes of wheat seedlings were investigated.201121791455
[the role of chromosomes from homeologous group 5 in regulation of grain hardness and protein content in substitution lines of common wheat cultivar saratovskaya 29].genetic regulation of grain hardness and protein content in intervarietal substitution lines for chromosomes of homeologous group 5 was examined. common wheat cultivar saratovskaya 29 with high bread-backing properties served as the recipient. donors of chromosomes 5a and 5d were 18 cultivars with variable traits examined, including high-protein cultivars (atlas 66 and diamant 2), and soft-grain cultivars (ul'yanovka and chinese spring). analysis of substitution lines pointed to a substantial ef ...201121786668
[ecotoxicity of synthetical musks on wheat (triticum aestivum) based on seed germination].with soil culture the ecotoxicological effects of galaxolide (hhcb) and tonalide (ahtn) on the germination of wheat (triticum aestivum) seeds were investigated. and the influence of these two synthetical musks on the wheat germination rate, inhibition rate of root elongation and inhibition rate of shoot elongation were studied. the results showed that there were no obvious dose-effect relationship between wheat germination rate and concentrations of these two synthetical musks. lower dose (hhcb ...201121780608
use of methylation filtration and c(0)t fractionation for analysis of genome composition and comparative genomics in bread wheat.we investigated the compositional and structural differences in sequences derived from different fractions of wheat genomic dna obtained using methylation filtration and c(0)t fractionation. comparative analysis of these sequences revealed large compositional and structural variations in terms of gc content, different structural elements including repeat sequences (e.g., transposable elements and simple sequence repeats), protein coding genes, and non-coding rna genes. a correlation between meth ...201121777856
deciphering the genetics of flowering time by an association study on candidate genes in bread wheat (triticum aestivum l.).earliness is very important for the adaptation of wheat to environmental conditions and the achievement of high grain yield. a detailed knowledge of key genetic components of the life cycle would enable an easier control by the breeders. the objective of the study was to investigate the effect of candidate genes on flowering time. using a collection of hexaploid wheat composed of 235 lines from diverse geographical origins, we conducted an association study for six candidate genes for flowering ...201121761163
pattern of solutes accumulated during leaf osmotic adjustment as related to duration of water deficit for wheat at the reproductive stage.this study examined expression of osmotic adjustment (oa) and accumulation of solutes in wheat (triticum aestivum l.) leaves in response to water deficit (wd) imposed at the reproductive stage. two contrasting cultivars, hartog and sunco (putatively high and low in oa capacity, respectively), were grown in deep (viz. 80 cm) pots in a controlled environment. in a sandy substrate, leaf oa was 5-times greater in hartog compared with sunco. at 21 d of wd treatment, k(+) only accounted for 12% of oa ...201121741263
modeling and phylogeny analysis of bread wheat mnsod.superoxide dismutase (sod) acts as first line of defense against oxidative and genetic stress. manganese superoxide dismutase (mnsod), found in mitochondria or peroxisomes, contains mn(iii) at the active site. therefore, it is of interest to study mnsod from bread wheat (a grain crop). however, a structure model is not yet solved for bread wheat mnsod. hence, we describe the structure model of bread wheat mnsod developed using homology model. the model provides molecular insight to metal binding ...201121738317
inhibition by wheat sprout (triticum aestivum) juice of bisphenol a-induced oxidative stress in young women.for health of future generation, fertile young women should be monitored for exposure of endocrine disrupting chemicals (edcs). among edcs, bisphenol a (bpa) is suggested to induce reactive oxygen species (ros) which play an important role in pathologies of female diseases such as endometriosis. on the other hand, previous studies suggested that sprouts of wheat (triticum aestivum) have antimutagenicity and antioxidant activity. we performed the 2 weeks intervention of wheat sprout juice (100ml/ ...201121736952
effects of sterol-binding agent nystatin on wheat roots: the changes in membrane permeability, sterols and glycoceramides.plant sterols are important multifunctional lipids, which are involved in determining membrane properties. biophysical characteristics of model lipid and isolated animal membranes with altered sterol component have been intensively studied. in plants however, the precise mechanisms of involvement of sterols in membrane functioning remain unclear. in present work the possible interactions between sterols and other membrane lipids in plant cells were studied. a useful experimental approach for elu ...201121726881
heterotrimeric gα subunit from wheat (triticum aestivum), ga3, interacts with the calcium-binding protein, clo3, and the phosphoinositide-specific phospholipase c, pi-plc1.the canonical gα subunit of the heterotrimeric g protein complex from wheat (triticum aestivum), ga3, and the calcium-binding protein, clo3, were revealed to interact both in vivo and in vitro and clo3 was shown to enhance the gtpase activity of ga3. clo3 is a member of the caleosin gene family in wheat with a single ef-hand domain and is induced during cold acclimation. bimolecular fluorescent complementation (bifc) was used to localize the interaction between clo3 and ga3 to the plasma membran ...201121725861
field controlled experiments of mercury accumulation in crops from air and soil.field open top chambers (otcs) and soil mercury (hg) enriched experiments were employed to study the influence of hg concentrations in air and soil on the hg accumulation in the organs of maize (zea mays l.) and wheat (triticum aestivum l.). results showed that hg concentrations in foliages were correlated significantly (p < 0.05) with air hg concentrations but insignificantly correlated with soil hg concentrations, indicating that hg in crop foliages was mainly from air. hg concentrations in ro ...201121723013
identification of differentially expressed genes in three-pistil mutation in wheat using annealing control primer system.the common wheat line three-pistil (tp) is a valuable mutant for wheat breeding. the tp mutation has normal spike morphology; however, it only produces three pistils per floret. therefore, it has potential to increase the grain number per spike. in order to determine the underlying molecular mechanism, an annealing control primer system was used to identify the different expressed genes in three-pistil mutation. using 120 arbitrary acp primers, we identified three differentially expressed genes ...201121722717
assessment of genetic diversity of wheat genotypes by resistance gene analog-est markers.resistance gene analog-expressed sequence tag (rga-est)-based markers have been used for variety discrimination and studies of genetic diversity in wheat. our aim is to increase the competitiveness of public wheat breeding programs through intensive use of modern selection technologies, mainly marker-assisted selection. the genetic diversity of 77 wheat nucleotide binding site (nbs)-containing rga-ests was assessed. resistant and susceptible bread wheat (triticum aestivum) genotypes were used as ...201121710462
distribution of gluten proteins in bread wheat (triticum aestivum) grain.gluten proteins are the major storage protein fraction in the mature wheat grain. they are restricted to the starchy endosperm, which forms white flour on milling, and interact during grain development to form large polymers which form a continuous proteinaceous network when flour is mixed with water to give dough. this network confers viscosity and elasticity to the dough, enabling the production of leavened products. the starchy endosperm is not a homogeneous tissue and quantitative and qualit ...201121693664
nema, a functional-structural model of nitrogen economy within wheat culms after flowering. i. model description.models simulating nitrogen use by plants are potentially efficient tools to optimize the use of fertilizers in agriculture. most crop models assume that a target nitrogen concentration can be defined for plant tissues and formalize a demand for nitrogen, depending on the difference between the target and actual nitrogen concentrations. however, the teleonomic nature of the approach has been criticized. this paper proposes a mechanistic model of nitrogen economy, nema (nitrogen economy model with ...201121685431
nema, a functional-structural model of nitrogen economy within wheat culms after flowering. ii. evaluation and sensitivity analysis.simulating nitrogen economy in crop plants requires formalizing the interactions between soil nitrogen availability, root nitrogen acquisition, distribution between vegetative organs and remobilization towards grains. this study evaluates and analyses the functional-structural and mechanistic model of nitrogen economy, nema (nitrogen economy model within plant architecture), developed for winter wheat (triticum aestivum) after flowering.201121685429
profiles of phenolic compounds in modern and old common wheat varieties determined by liquid chromatography coupled with time-of-flight mass spectrometry.the health-promoting properties of common wheat (triticum aestivum l.) have been largely attributed to the presence of unique phytochemicals of whole grains. the aim of this study was to profile the phenolic content of 16 old and 6 modern italian wheat varieties, cropped in the same location and growing season. high variability was observed among the investigated wheat genotypes, both in the free and bound phenolic extracts. the total polyphenol content ranged from 885.5 to 1715.9 μmol gae/100 g ...201121683368
molecular cloning and variation of ω-gliadin genes from a somatic hybrid introgression line ii-12 and parents (triticum aestivum cv. jinan 177 and agropyron elongatum). 201121677400
physical localization of nors and its length variants in old portuguese durum wheat cultivars.the variation at the internal transcribed spacer (its) region of the ribosomal dna has been correlated with the number of nucleolar organizer regions (nors) in some plant species. besides, the number of nors might influence the rate of homogenization of the rdna repeats. in recent studies, its length variants were detected in bread wheat cultivars but no reports about their presence in durum wheat were found. in the present study, we localized and identified the nors of 51 old portuguese durum w ...201121677393
[effects of nitrogen application and elevated atmospheric co2 on electron transport and energy partitioning in flag leaf photosynthesis of wheat].wheat (triticum aestivum) plants were pot-cultured in open top chambers at the nitrogen application rate of 0 and 200 mg x kg(-1) soil and the atmospheric co2 concentration of 400 and 760 micromol x mol(-1). through the determination of flag leaf nitrogen and chlorophyll contents, photosynthetic rate (pn)-intercellar co2 concentration (ci) response curve, and chlorophyll fluorescence parameters at heading stage, the photosynthetic electron transport rate and others were calculated, aimed to inve ...201121657023
association mapping of dynamic developmental plant height in common wheat.drought as a major abiotic stress often occurs from stem elongation to the grain filling stage of wheat in northern china. plant height (ph) is a suitable trait to model the dissection of drought tolerance. the purposes of the present study were to validate molecular markers for ph developmental behavior and identify elite alleles of molecular markers. after the phenotyping of 154 accessions for ph dynamic development under well-watered (ww) and drought stressed (ds) conditions, and the genotypi ...201121647605
autoimmune response and repression of mitotic cell division occur in inter-specific crosses between tetraploid wheat and aegilops tauschii coss. that show low temperature-induced hybrid necrosis.common wheat is an allohexaploid species originating from a naturally occurring inter-specific cross between tetraploid wheat and the diploid wild wheat aegilops tauschii coss. artificial allopolyploidization can produce synthetic hexaploid wheat. however, synthetic triploid hybrids show four types of hybrid growth abnormalities: type ii and iii hybrid necrosis, hybrid chlorosis, and severe growth abortion. of these hybrid abnormalities, type ii necrosis is induced by low temperature. under low ...201121645146
the effect of extremely diluted agitated gibberellic acid (10e-30) on wheat stalk growth--a two researcher pilot study.use of a wheat growth bio assay after 7 days in research on homeopathic dilutions of gibberellic acid.201121641523
automated flow-based anion-exchange method for high-throughput isolation and real-time monitoring of rubisco in plant extracts.in this work, a miniaturized, completely enclosed multisyringe-flow system is proposed for high-throughput purification of rubisco from triticum aestivum extracts. the automated method capitalizes on the uptake of the target protein at 4°c onto q-sepharose fast flow strong anion-exchanger packed in a cylindrical microcolumn (105 × 4 mm) followed by a stepwise ionic-strength gradient elution (0-0.8 mol/l nacl) to eliminate concomitant extract components and retrieve highly purified rubisco. the m ...201121641435
dynamic management of crop diversity: from an experimental approach to on-farm conservation.in agricultural systems, biodiversity includes diversity within species and among species and provides many benefits for production, resilience and conservation. this article addresses the effects of a strategy of in situ conservation called dynamic management (dm) on population evolution, adaptation and diversity. two french dm initiatives are considered, the first one corresponding to an experimental context, the second to an on-farm management. results from a study over 26 years of experiment ...201121640955
genetic diversity analysis of abiotic stress response gene tasnrk2.7-a in common wheat.sucrose non-fermenting1-related protein kinase 2 (snrk2) plays a key role in plant stress signaling transduction pathways. in this study, one copy of tasnrk2.7, a snrk2 member of common wheat, was isolated and characterized for nucleotide diversity among 45 wheat accessions with different stress-response features. most of the accessions were elite wheat cultivars, which had been subject to population bottlenecks and intensive selection during breeding. nucleotide and haplotype diversity across t ...201121637995
effects of high temperature after anthesis on starch granules in grains of wheat (triticum aestivum l.).the effect of high temperatures (above 25°c) on starch concentration and the morphology of starch granules in the grains of wheat (triticum aestivum l.) were studied. wheat plants of cultivars yangmai 9 (weak-gluten) and yangmai 12 (medium-gluten) were treated with high temperatures for 3 days at different times after anthesis. the results showed that the starch concentration of grains given a heat-shock treatment above 30°c were lower than those developing at normal temperature in both cultivar ...201122505772
color of whole-wheat foods prepared from a bright-white hard winter wheat and the phenolic acids in its coarse bran.the color of wheat kernels often impacts the color and thereby the value of wheat-based foods. a line of hard white winter wheat (b-w hw) with bright appearing kernels has been developed at the kansas state agricultural research center. the objective of this study was to compare the color of several foods made from the b-w hw wheat with those of 2 hard white wheat cultivars, trego and lakin. the b-w hw kernels showed higher lightness (l*, 57.6) than trego (55.5) and lakin (56.8), and the increas ...201122417482
[molecular genetic characteristics of the wx-b1e allele from common wheat and applicability of the dna markers for its identification].molecular genetic characterization of the wx-b1e allele identified by the authors of the study in the common wheat cultivar korotyshka was performed. the 804-bp wx-b1e fragment was cloned and sequenced. comparison of the sequence obtained with that for the wild-type allele of common wheat (wx-b1a) demonstrated that wx-b1e carried the 34-bp insertion, 8-bp deletion, and 23 nucleotide substitutions. blast analysis revealed the highest homology with the nucleotide sequences of wx genes from triticu ...201122384689
variability of gene expression after polyhaploidization in wheat (triticum aestivum l.).interspecific hybridization has a much greater effect than chromosome doubling on gene expression; however, the associations between homeologous gene expression changes and polyhaploidization had rarely been addressed. in this study, cdna-single strand conformation polymorphism analysis was applied to measure the expression of 30 homeologous transcripts in naturally occurring haploid (abd, 2n = 21) and its polyploid maternal parent yumai 21a (aabbdd, 2n = 42) in wheat. only one gene (tc251989) s ...201122384315
[combination effects of enhanced uv-b radiation and o3 stress on photosynthetic characteristics of winter wheat].experiments were conducted under open-top-chambers conditions to assess the photosynthetic responses of wheat plants (triticum aestivum l., yangmail6) to supplemental uv-b radiation (10%-10.9% higher then control group, t1) and enhanced ozone [(100 +/- 9) nmol x mol(-1), t2], separately and in combination (combination treatment, t3), making use of lcpro + portable photosynthesis system and diving-pam fluorometer to determine gas exchange and chlorophyll fluorescence parameters. results indicated ...201122279919
[contribution of wheat rhizosphere respiration to soil respiration under elevated atmospheric co2 and nitrogen application].with the support of free-air carbon dioxide enrichment (face) system and by using isotope 13c technique, and through planting wheat (triticum aestivum l., c3 crop) on a soil having been planted with maize (zea mays l., c4 crop) for many years, this paper studied the effects of elevated atmospheric co2 and nitrogen application on the delta 13c value of soil emitted co2 and the wheat rhizosphere respiration. with the growth of wheat, the delta 13c value of soil emitted co2 had a gradual decrease. ...201122263454
[effects of irrigation on the water soluble carbohydrate contents in different wheat cultivars stem and sheath and the grain yield].taking two wheat (triticum aestivum l.) cultivars jimai 20 and taishan 22 as test objects, this paper studied the effects of different irrigation treatments on the water soluble carbohydrate contents in penult stem internode and sheath and the grain yield. four irrigation treatments were installed, i. e. , no irrigation (w0), irrigation at overwintering and jointing stages (w1), irrigation at overwintering, jointing and anthesis stages (w2), and irrigation at overwintering, jointing, anthesis an ...201122263448
analysis of the spatio-temporal distribution of eurygaster integriceps (hemiptera: scutelleridae) by using spatial analysis by distance indices and geostatistics.eurygaster integriceps puton (hemiptera: scutelleridae) is the most serious insect pest of wheat (triticum aestivum l.) and barley (hordeum vulgare l.) in iran. in this study, spatio-temporal distribution of this pest was determined in wheat by using spatial analysis by distance indices (sadie) and geostatistics. global positioning and geographic information systems were used for spatial sampling and mapping the distribution of this insect. the study was conducted for three growing seasons in gh ...201122251736
gene flow in genetically modified wheat.understanding gene flow in genetically modified (gm) crops is critical to answering questions regarding risk-assessment and the coexistence of gm and non-gm crops. in two field experiments, we tested whether rates of cross-pollination differed between gm and non-gm lines of the predominantly self-pollinating wheat triticum aestivum. in the first experiment, outcrossing was studied within the field by planting "phytometers" of one line into stands of another line. in the second experiment, outcro ...201122216349
nucleotide diversity and molecular evolution of the wag-2 gene in common wheat (triticum aestivum l) and its relatives.in this work, we examined the genetic diversity and evolution of the wag-2 gene based on new wag-2 alleles isolated from wheat and its relatives. only single nucleotide polymorphisms (snp) and no insertions and deletions (indels) were found in exon sequences of wag-2 from different species. more snps and indels occurred in introns than in exons. for exons, exons+introns and introns, the nucleotide polymorphism π decreased from diploid and tetraploid genotypes to hexaploid genotypes. this finding ...201122215965
heteroplasmy and expression of mitochondrial genes in alloplasmic and euplasmic wheat.the plant chondriome confers a complex nature. the atp4 gene (formerly called orf25) of aegilops crassa (cr) harbors the promoter sequence of the rps7 gene from common wheat (triticum aestivum cv. chinese spring, cs). the rps7 gene of cr has the promoter sequence of cs atp6. the atp6 gene of cr contains an unknown sequence inside of its coding region. since repeat sequences have been found around the breaking points, these structural alterations are most likely generated through homologous recom ...201122214593
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