Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| melioidosis imported from west africa to europe. | abstract. we report the first case of imported melioidosis in spain from a diabetic immigrant who visited west africa during the rainy season. because of the unusual presentation of this disease in africa, clinical and microbiological diagnosis of imported melioidosis from this continent can be very elusive. | 2011 | 21813848 |
| cable pili and the associated 22 kda adhesin contribute to burkholderia cenocepacia persistence in vivo. | infection by burkholderia cenocepacia in cystic fibrosis (cf) patients is associated with poor clinical prognosis. previously, we demonstrated that one of the highly transmissible strains, bc7, expresses cable pili and the associated 22 kda adhesin, both of which contribute to bc7 binding to airway epithelial cells. however, the contribution of these factors to induce inflammation and bacterial persistence in vivo is not known. | 2011 | 21811611 |
| extreme antimicrobial peptide and polymyxin b resistance in the genus burkholderia. | cationic antimicrobial peptides and polymyxins are a group of naturally occurring antibiotics that can also possess immunomodulatory activities. they are considered a new source of antibiotics for treating infections by bacteria that are resistant to conventional antibiotics. members of the genus burkholderia, which includes various human pathogens, are inherently resistant to antimicrobial peptides. the resistance is several orders of magnitude higher than that of other gram-negative bacteria s ... | 2011 | 21811491 |
| autotransporters and their role in the virulence of burkholderia pseudomallei and burkholderia mallei. | burkholderia pseudomallei and burkholderia mallei are closely related gram-negative bacteria responsible for the infectious diseases melioidosis and glanders, respectively. autotransporters (ats) comprise a large and diverse family of secreted and outer membrane proteins that includes virulence-associated invasins, adhesins, proteases, and actin-nucleating factors. the b. pseudomallei k96243 genome contains 11 predicted ats, eight of which share homologs in the b. mallei atcc 23344 genome. this ... | 2011 | 21811486 |
| the burkholderia pseudomallei {delta}asd mutant exhibits attenuated intracellular infectivity and imparts protection against acute inhalation melioidosis in mice. | burkholderia pseudomallei, the cause of serious and life threatening diseases in humans, is of national biodefense concern because of its potential use as a bioterrorism agent. this microbe is listed as a select agent by the cdc, therefore, development of vaccines is of significant importance. here, we further investigated the growth characteristics of a recently created b. pseudomallei 1026b ?asd mutant in vitro, in a cell model, and in an animal model of infection. the mutant was typified by a ... | 2011 | 21807903 |
| genome and characterization of phages bcep22 and bcepil02, founders of a novel phage type in burkholderia cenocepacia. | within the burkholderia cepacia complex (bcc), b. cenocepacia is the most common species associated with aggressive infections in the lungs of cystic fibrosis patients, causing disease that is often refractive to treatment by antibiotics. phage therapy may be a potential alternative form of treatment for these infections. here we describe the genome of the previously described therapeutic b. cenocepacia podophage bcepil02 and its close relative, bcep22. phage bcep22 was found to contain a circul ... | 2011 | 21804006 |
| highly sensitive direct detection and quantification of burkholderia pseudomallei in environmental soil samples using real-time pcr. | the soil bacterium and potential biothreat agent burkholderia pseudomallei causes the infectious disease melioidosis, which is naturally acquired through environmental contact with the bacterium. environmental detection of b. pseudomallei represents the basis for the development of a geographical risk map to humans and livestock. the aim of the present study was to develop a highly sensitive culture-independent dna-based method that allows direct quantification of b. pseudomallei from soil. we e ... | 2011 | 21803915 |
| endovascular therapy for infected aortic aneurysms. | objective: to determine the outcome of endovascular therapy for an infected aortic aneurysm in patients with or without aorto-aerodigestive/aortocaval fistulas. methods: from september 2005 to may 2010, 21 patients, 17 abdominal and four thoracic infected aortic aneurysms were treated with an endovascular stent graft at songklanagarind hospital, thailand. five patients presented with fistula complications, 1 aortoesophageal, 1 aortobronchial, 1 aortocaval, and 2 aortoenteric fistulas. lifelong a ... | 2011 | 21802238 |
| structural and functional analysis of phytotoxin toxoflavin-degrading enzyme. | pathogenic bacteria synthesize and secrete toxic low molecular weight compounds as virulence factors. these microbial toxins play essential roles in the pathogenicity of bacteria in various hosts, and are emerging as targets for antivirulence strategies. toxoflavin, a phytotoxin produced by burkholderia glumae bgr1, has been known to be the key factor in rice grain rot and wilt in many field crops. recently, toxoflavin-degrading enzyme (txde) was identified from paenibacillus polymyxa jh2, there ... | 2011 | 21799856 |
| neutrophils are the predominant cell-type to associate with burkholderia pseudomallei in a balb/c mouse model of respiratory melioidosis. | a variety of studies have implicated neutrophils and the rapid induction of cytokine in the host response in melioidosis. here a balb/c mouse model of infection with aerosolised burkholderia pseudomalleik96243 has been used to understand the immune response to infection in this model and verify other infection models that show rapid growth of bacteria, colonisation of tissues and periphery, induction of cytokines and influx of neutrophils. uniquely, this study has also determined the association ... | 2011 | 21798336 |
| thailandepsins: bacterial products with potent histone deacetylase inhibitory activities and broad-spectrum antiproliferative activities. | histone deacetylase (hdac) inhibitors have emerged as a new class of anticancer drugs, with one synthetic compound, saha (vorinostat, zolinza; 1), and one natural product, fk228 (depsipeptide, romidepsin, istodax; 2), approved by fda for clinical use. our studies of fk228 biosynthesis in chromobacterium violaceum no. 968 led to the identification of a cryptic biosynthetic gene cluster in the genome of burkholderia thailandensis e264. genome mining and genetic manipulation of this gene cluster fu ... | 2011 | 21793558 |
| combining vaccination and postexposure cpg therapy provides optimal protection against lethal sepsis in a biodefense model of human melioidosis. | the gram-negative bacterium burkholderia pseudomallei is the causative agent of melioidosis, a major cause of lethal sepsis and morbidity in endemic areas of southeast asia and a potential bioterrorism threat. we have used susceptible balb/c mice to evaluate the potential of targeting vaccination and generic immunotherapy to the lung for optimal protection against respiratory challenge. intranasal vaccination with live attenuated b. pseudomallei increased survival and induced interferon-?-secret ... | 2011 | 21791666 |
| isolation and characterization of a novel virulent podovirus which infects burkholderia pseudomallei. | abstract: burkholderia pseudomallei is a saprophytic soil bacterium and the etiological agent that causes melioidosis. it is naturally resistant to many antibiotics and therefore is difficult to treat. bacteriophages may provide an alternative source of treatment. we have isolated and characterised the bacteriophage phi bp-amp1. the phage is a member of the podoviridae family and has a genome size of ~ 45 kb. molecular data based on the gene which encodes for the phage tail tubular protein sugge ... | 2011 | 21791081 |
| improvement of anti-burkholderia mouse monoclonal antibody from various phage-displayed single-chain antibody libraries. | to improve anti-burkholderia monoclonal antibody (mab) binding affinity, six single chain variable fragments (scfvs) constructed previously were used as scaffolds to construct large highly-diversified phage-displayed mouse scfv random and domain libraries. first, we employed random mutagenesis to introduce random point mutations into entire variable regions, generating six random libraries. additionally, the oligonucleotide-directed mutagenesis was targeted on complementarity-determining region ... | 2011 | 21787781 |
| burkholderia pseudomallei-induced cell fusion in u937 macrophages can be inhibited by monoclonal antibodies against host cell surface molecules. | burkholderia pseudomallei induces the formation of multinucleated giant cells in cell monolayers. after infection of human macrophage-like u937 cells with b. pseudomallei, addition of monoclonal antibodies against integrin-associated protein (cd47), e-selectin (cd62e), a fusion regulatory protein (cd98), and e-cadherin (cd324) suppressed multinucleated giant cells in a concentration-dependent manner while monoclonal antibodies against other surface molecules did not inhibit fusion despite bindin ... | 2011 | 21784167 |
| antimicrobial activity of cathelicidin peptides against burkholderia pseudomallei, the causative agent of melioidosis. | 2011 | 21782393 | |
| spontaneous and evolutionary changes in the antibiotic resistance of burkholderia cenocepacia observed by global gene expression analysis. | abstract: background: burkholderia cenocepacia is a member of the burkholderia cepacia complex group of bacteria that cause infections in individuals with cystic fibrosis. b. cenocepacia isolate j2315 has been genome sequenced and is representative of a virulent, epidemic cf strain (et12). its genome encodes multiple antimicrobial resistance pathways and it is not known which of these is important for intrinsic or spontaneous resistance. to map these pathways, transcriptomic analysis was perform ... | 2011 | 21781329 |
| blocking candida albicans biofilm formation by bdsf and trans-bdsf. | candida is an important opportunistic human fungal pathogen. infections caused by c. albicans are related to the formation of a biofilm. the biofilm enhances the resistance of the c. albicans defense system, increases its resistance to antifungal drugs, and induces increased drug tolerance and makes clinical care more challenging. the in vitro activity of bdsf (cis-2-dodecenoic acid) and trans-bdsf (trans-2-dodecenoic acid) against candida albicans growth, germ tube germination and biofilm forma ... | 2011 | 21778264 |
| toll-like receptor 4 region genetic variants are associated with susceptibility to melioidosis. | melioidosis is a tropical infection caused by the gram-negative soil saprophyte burkholderia pseudomallei. despite broad exposure of northeastern thais, disease develops in only a small proportion of individuals. although diabetes is a risk factor, the mechanisms of host susceptibility to melioidosis are still poorly understood. we postulated that toll-like receptors (tlrs) regulate host susceptibility to disease, and that genetic variation in tlrs is associated with melioidosis. we analyzed the ... | 2011 | 21776015 |
| in vitro activity of sitafloxacin against burkholderia pseudomallei. | 2011 | 21775108 | |
| multi-functional characteristics of the pseudomonas aeruginosa type iii needle-tip protein, pcrv; comparison to orthologs in other gram-negative bacteria. | pseudomonas aeruginosa possesses a type iii secretion system (t3ss) to intoxicate host cells and evade innate immunity. this virulence-related machinery consists of a molecular syringe and needle assembled on the bacterial surface, which allows delivery of t3 effector proteins into infected cells. to accomplish a one-step effector translocation, a tip protein is required at the top end of the t3 needle structure. strains lacking expression of the functional tip protein fail to intoxicate host ce ... | 2011 | 21772833 |
| an outbreak of burkholderia cepacia complex associated with intrinsically contaminated nasal spray. | objective. to determine the source of burkholderia cepacia complex associated with a hospital outbreak and describe the measures taken to identify and confirm the source. setting. a 250-bed, tertiary care pediatric hospital in denver, colorado. methods. an epidemiologic investigation was used to identify possible causes for an apparent outbreak of b. cepacia complex in pediatric patients who had new positive cultures with this organism from december 2003 to february 2004. chart review, microbiol ... | 2011 | 21768765 |
| caenorhabditis elegans npr-1-mediated behaviors are suppressed in the presence of mucoid bacteria. | caenorhabditis elegans exhibits a diverse range of behaviors in response to bacteria. the presence of bacterial food influences c. elegans aerotaxis, aggregation, locomotion, and pathogen avoidance behaviors through the activity of the npr-1 neuropeptide receptor. here, we show that mucoid strains of bacteria that produce an exopolysaccharide matrix do not induce npr-1-dependent behaviors. in the presence of mucoid strains of bacteria, the c. elegans laboratory wild-type (wt) strain n2 exhibits ... | 2011 | 21768378 |
| a role for the burkholderia pseudomallei type three secretion system cluster 1 bpscn gene in virulence. | burkholderia pseudomallei, the causal agent of melioidosis, employs a number of virulence factors during its infection of mammalian cells. one such factor is the type three secretion system (ttss), which is proposed to mediate the transport and secretion of bacterial effector molecules directly into host cells. the b. pseudomallei genome contains three ttss gene clusters (designated ttss1, ttss2 and ttss3). previous research has indicated that neither ttss1 nor ttss2 are involved in b. pseudomal ... | 2011 | 21768285 |
| development of a colorimetric colony-screening assay for detection of defluorination by micro-organisms. | aims: to develop a colorimetric colony-screening assay to facilitate the isolation of micro-organisms capable of defluorination. methods and results: a metal-dye chelate, zirconium-xylenol orange was used to detect fluoride ions released from a fluorinated substrate through microbial metabolism. depolymerised zirconium reagent gave the greatest visual contrast for the presence of fluoride compared to more polymerised forms of zirconium reagent. the sensitivity of the assay was greatest when th ... | 2011 | 21767280 |
| survival of burkholderia pseudomallei in distilled water for 16 years. | burkholderia pseudomallei was examined after being maintained in distilled water at 25°c for 16 years. the gram stain was atypical (pale pink cocci or coccobacilli). the estimated number of live and dead b. pseudomallei was 3.8×10(7) cells/ml and 1.4×10(5) cells/ml, respectively. a colony count on agar of 1.0×10(6) cfu/ml suggested that a proportion of cells were in a viable but non-culturable state. colony morphology was different from the parental isolate for 84% of colonies. pulsed-field gel ... | 2011 | 21764093 |
| etymologia: melioidosis. | from the greek melis, distemper of asses, oeide-s, resemblance, and osis, a suffix indicating an abnormal condition or disease. alfred whitmore, a british pathologist serving in burma, and his assistant c. s. krishnaswami first described melioidosis in 1912. the infection became known as whitmore's disease. in 1925, ambrose t. stanton and william fletcher, the researchers who identified burkholderia pseudomallei as the infection's causative agent, renamed the infection melioidosis because of its ... | 2011 | 21762621 |
| typhoon-related leptospirosis and melioidosis, taiwan, 2009. | to the editor: global climatic changes have resulted in more natural disasters worldwide. these natural disasters can then cause outbreaks of emerging infectious diseases, including leptospirosis and melioidosis (1-7). in 2009, the moderate-strength typhoon morakot, with a maximum cumulative rainfall amount up to 3,059.5 mm, damaged taiwan. after this natural disaster, unusual epidemics of leptospirosis and melioidosis occurred. the main objective of this study was to clarify whether these epide ... | 2011 | 21762606 |
| melioidosis in birds and burkholderia pseudomallei dispersal, australia. | to the editor: melioidosis is an emerging infectious disease of humans and animals caused by the gram-negative bacterium burkholderia pseudomallei, which inhabits soil and surface water in the disease-endemic regions of southeast asia and northern australia (1). the aim of this study was to assess the potential for birds to spread b. pseudomallei. birds are known carriers of various human pathogens, including influenza viruses, west nile virus, campylobacter jejuni, and antimicrobial drug-resist ... | 2011 | 21762599 |
| melioidosis acquired by traveler to nigeria. | we describe melioidosis associated with travel to nigeria in a woman with diabetes, a major predisposing factor for this infection. with the prevalence of diabetes projected to increase dramatically in many developing countries, the global reach of melioidosis may expand. | 2011 | 21762592 |
| melioidosis, phnom penh, cambodia. | we describe 58 adult patients with melioidosis in cambodia (2007-2010). diabetes was the main risk factor (59%); 67% of infections occurred during the rainy season. bloodstream infection was present in 67% of patients, which represents 12% of all bloodstream infections. the case-fatality rate was 52% and associated with inappropriate empiric treatment. | 2011 | 21762590 |
| epidemiology and investigation of melioidosis, southern arizona. | burkholderia pseudomallei is a bacterium endemic to southeast asia and northern australia, but it has not been found to occur endemically in the united states. we report an ostensibly autochthonous case of melioidosis in the united states. despite an extensive investigation, the source of exposure was not identified. | 2011 | 21762589 |
| burkholderia pseudomallei in unchlorinated domestic bore water, tropical northern australia. | to determine whether unchlorinated bore water in northern australia contained burkholderia pseudomallei organisms, we sampled 55 bores; 18 (33%) were culture positive. multilocus sequence typing identified 15 sequence types. the b. pseudomallei sequence type from 1 water sample matched a clinical isolate from a resident with melioidosis on the same property. | 2011 | 21762588 |
| natural burkholderia mallei infection in dromedary, bahrain. | we confirm a natural infection of dromedaries with glanders. multilocus variable number tandem repeat analysis of a burkholderia mallei strain isolated from a diseased dromedary in bahrain revealed close genetic proximity to strain dubai 7, which caused an outbreak of glanders in horses in the united arab emirates in 2004. | 2011 | 21762586 |
| phb biosynthesis in catabolite repression mutant of burkholderia sacchari. | due to the effect of catabolite repression, sugar mixtures cannot be metabolized in a rapid and efficient way implicating in lower productivity in bioprocesses using lignocellulosic hydrolysates. in gram-negative bacteria, this mechanism is mediated by the phosphotransferase system (pts), which concomitantly internalizes and phosphorylates sugars. in this study, we isolated a uv mutant of burkholderia sacchari, called lfm828, which transports hexoses and pentoses by a non-pts uptake system. this ... | 2011 | 21761218 |
| a recombinant burkholderia intracellular motility a (bima) protein for immunodiagnosis of glanders. | glanders, caused by the gram-negative, nonmotile bacterium burkholderia mallei, is a contagious and highly fatal disease of equines. during the last decade number of glanders outbreaks has increased steadily. the disease also has high zoonotic significance and b. mallei is listed biological warfare agent. complement fixation test (cft) is a routinely used and internationally recognised test to screen equine sera for the glanders. however, discrepant results have been observed by cft. the low sen ... | 2011 | 21752949 |
| burkholderia multivorans acts as an antagonist against the growth of burkholderia pseudomallei in soil. | burkholderia multivorans nki379 has an antagonistic effect against the growth of b. pseudomallei, which we have demonstrated here using agar diffusion tests and a transwell system. bacterial representatives were isolated from agricultural crop soil and mixed to construct a partial bacterial community structure that based on the results of reproducible patterns following pcr-dgge (denaturing gradient gel electrophoresis) analysis from total soil chromosomes. the antagonistic effect of b. multivor ... | 2011 | 21752084 |
| in vivo fluorescence imaging of bacteriogenic cyanide in the lungs of live mice infected with cystic fibrosis pathogens. | pseudomonas aeruginosa (pa) and burkholderia cepacia complex (bcc), commonly found in the lungs of cystic fibrosis (cf) patients, often produce cyanide (cn), which inhibits cellular respiration. cn in sputa is a potential biomarker for lung infection by cf pathogens. however, its actual concentration in the infected lungs is unknown. | 2011 | 21750709 |
| infection in immunocompromised hosts and the organ transplant recipient -essentials. | 2011 | 21748845 | |
| molecular investigations of pena-mediated ß-lactam resistance in burkholderia pseudomallei. | burkholderia pseudomallei is the etiological agent of melioidosis. because of the bacterium's intrinsic resistance and propensity to establish latent infections, melioidosis therapy is complicated and prolonged. newer generation ß-lactams, specifically ceftazidime, are used for acute phase therapy, but resistance to this cephalosporin has been observed. the chromosomally encoded pena gene encodes a putative twin arginine translocase (tat)-secreted ß-lactamase, and pena mutations have been implic ... | 2011 | 21747814 |
| activation of mmp-9 by human lung epithelial cells in response to cystic fibrosis associated pathogen burkholderia cenocepacia reduced wound healing in vitro. | burkholderia cepacia complex is a group of bacterial pathogens which cause opportunistic infections in cystic fibrosis (cf). the most virulent of these is burkholderia cenocepacia. matrix metalloproteases (mmps) are upregulated in cf patients. the aim of this work was to examine the role of mmps in the pathogenesis of b. cepacia complex, which has not been explored to date. real-time pcr analysis showed that b. cenocepacia infection up-regulated mmp-2 and mmp-9 genes in cf lung cell line, cfbe41 ... | 2011 | 21743026 |
| diversity analysis of diazotrophic bacteria associated with the roots of tea (camellia sinensis (l.) o. kuntze). | the diversity elucidation by amplified ribosomal dna restriction analysis and 16s rdna sequencing of 96 associative diazotrophs, isolated from the feeder roots of tea on enriched nitrogen-free semisolid media, revealed the predominance of gram-positive over gram-negative bacteria within the kangra valley in himachal pradesh, india. the gram-positive bacteria observed belong to two taxonomic groupings; firmicutes, including the genera bacillus and paenibacillus; and actinobacteria, represented by ... | 2011 | 21715960 |
| the dioxygenase-encoding olsd gene from burkholderia cenocepacia causes the hydroxylation of the amide-linked fatty acyl moiety of ornithine-containing membrane lipids. | burkholderia cenocepacia is an important opportunistic pathogen, and one of the most striking features of the burkholderia genus is the collection of polar lipids present in its membrane, including phosphatidylethanolamine (pe) and ornithine-containing lipids (ols), as well as the 2-hydroxylated derivatives of pe and ols (2-oh-pe and 2-oh-ols, respectively), which differ from the standard versions by virtue of the presence of a hydroxyl group at c2 (2-oh) of an esterified fatty acyl residue. sim ... | 2011 | 21707055 |
| evidence of burkholderia pseudomallei-specific immunity in patient sera persistently nonreactive by the indirect hemagglutination assay. | the indirect hemagglutination assay (iha) is the most frequently used serological test to confirm exposure to burkholderia pseudomallei. patients with culture-confirmed disease often have a nonreactive iha at presentation and occasionally fail to seroconvert on serial testing. we investigated whether using antigens derived from the cultured isolates of persistently iha-nonreactive patient sera improved the sensitivity of the iha. in addition, we assessed the antigen-specific lymphocyte response ... | 2011 | 21677111 |
| biosynthesis of novel polyhydroxyalkanoate containing 3-hydroxy-4-methylvalerate by chromobacterium sp. usm2. | aims:ôçé polyhydroxyalkanoate (pha) with enhanced physicochemical properties will be ideal for a wide range of practical applications. the incorporation of 3-hydroxy-4-methylvalerate (3h4mv) into the polymer backbone is known to improve the overall properties of the resulting polymer. however, the most suitable micro-organism and pha synthase that can synthesize this monomer efficiently still remain unknown at present. therefore, we evaluated the abilities of a locally isolated chromobacterium s ... | 2011 | 21689225 |
| legume-nodulating betaproteobacteria: diversity, host range and future prospects. | rhizobia form specialized nodules on the roots of legumes (fabaceae) and fix nitrogen in exchange for carbon from the host plant. although the majority of legumes form symbioses with rhizobium and its relatives in the alphaproteobacteria, some legumes, such as those in the large genus mimosa, are nodulated predominantly by betaproteobacteria in the genera burkholderia and cupriavidus. the principal centers of diversity of these bacteria are in central brazil and south africa. molecular phylogene ... | 2011 | 21830951 |
| mucoid morphotype variation of burkholderia multivorans during chronic cystic fibrosis lung infection is correlated with changes in metabolism, motility, biofilm formation and virulence. | burkholderia cepacia complex (bcc) bacteria are opportunistic pathogens infecting hosts such as cystic fibrosis (cf) patients. bcc long-term infection of cf patient airways has been associated with emergence of phenotypic variation. here we studied two burkholderia multivorans clonal isolates displaying different morphotypes from a chronically infected cf patient to evaluate traits development during lung infection. expression profiling of mucoid d2095 and nonmucoid d2214 isolates revealed decre ... | 2011 | 21835880 |
| a multiplexed immunoassay system based upon reciprocating centrifugal microfluidics. | a novel, centrifugal disk-based micro-total analysis system (µtas) for low cost and high throughput semi-automated immunoassay processing was developed. a key innovation in the disposable immunoassay disk design is in a fluidic structure that enables very efficient micro-mixing based on a reciprocating mechanism in which centrifugal acceleration acting upon a liquid element first generates and stores pneumatic energy that is then released by a reduction of the centrifugal acceleration, resulting ... | 2011 | 21721711 |
| discovery of a metagenome-derived enzyme that produces branched-chain acyl-(acyl-carrier-protein)s from branched-chain +¦-keto acids. | 2011 | 21714057 | |
| impact of endofungal bacteria on infection biology, food safety, and drug development. | 2011 | 21738468 | |
| [a case of melioidosis occurring after a long-term stay in vietnam that developed pulmonary cavitation and relapsed with multiple pulmonary nodules]. | a 69-year-old man complained of fever in september 2009, after returning from vietnam where he has been working for 20 years. he had diabetes mellitus and was on diabetic oral medication. he was examined at a nearby hospital, and found out to have pneumonia with cavity formation in the right upper lobe which was found out to be not due to tuberculosis. although the patient once recovered with antibacterial medicine, after a few months, in january 2010, he was admitted to our hospital because of ... | 2011 | 21735746 |
| solvent dielectric effect and side chain mutation on the structural stability of burkholderia cepacia lipase active site: a quantum mechanical/molecular mechanics study. | quantum mechanical and molecular dynamics methods were used to analyze the structure and stability of neutral and zwitterionic configurations of the extracted active site sequence from a burkholderia cepacia lipase, histidyl-seryl-glutamin (his86-ser87-gln88) and its mutated form, histidyl-cysteyl-glutamin (his86-cys87-gln88) in vacuum and different solvents. the effects of solvent dielectric constant, explicit and implicit water molecules and side chain mutation on the structure and stability o ... | 2011 | 21710316 |
| low-dose exposure of c57bl/6 mice to burkholderia pseudomallei mimics chronic human melioidosis. | burkholderia pseudomallei is the etiological agent of human melioidosis, a disease with a broad spectrum of clinical manifestations ranging from fatal septicemia to chronic localized infection or asymptomatic latent infection. most clinical and immunological studies to date have focused on the acute disease process; however, little is known about pathology and immune response in chronic melioidosis. here, we have developed a murine model of chronic disease by challenging c57bl/6 mice intranasall ... | 2011 | 21703409 |
| [analysis of an outbreak of burkholderia cepacia for risk stratification]. | the possibility of nosocomial infection is determined by factors relating to the patient, the presence and degree of immunodeficiency, and interventions that become stronger the risk. a nosocomial outbreak is the emergence of an unusual case of a specific disease and is due to an infectious agent only. our objective was to analyze the risk of burkholderia cepacia with a risk stratification criterion in a pediatric intensive care unit (icu). | 2011 | 21703140 |
| small-molecule inhibitor binding to an n-acyl-homoserine lactone synthase. | quorum sensing (qs) controls certain behaviors of bacteria in response to population density. in gram-negative bacteria, qs is often mediated by n-acyl-l-homoserine lactones (acyl-hsls). because qs influences the virulence of many pathogenic bacteria, synthetic inhibitors of acyl-hsl synthases might be useful therapeutically for controlling pathogens. however, rational design of a potent qs antagonist has been thwarted by the lack of information concerning the binding interactions between acyl-h ... | 2011 | 21730159 |
| cascade bioreactor with submerged biofilm for aerobic treatment of tunisian landfill leachate. | a bioreactor cascade with a submerged biofilm is proposed to treat young landfill leachate of jbel chakir landfill site south west from capital tunis, tunisia. the prototype was run under different organic loading charges varying from 0.6 to 16.3kgtocm(-3)day(-1). without initial ph adjustment total organic carbon (toc) removal rate varied between 65% and 97%. the total reduction of cod reached 92% at a hydraulic retention time of 36h. however, the removal of total kjeldahl nitrogen for loading ... | 2011 | 21703853 |
| incidence of bacteremic melioidosis in eastern and northeastern thailand. | burkholderia pseudomallei, the causative agent of melioidosis, is endemic in northeastern thailand. population-based disease burden estimates are lacking and limited data on melioidosis exist from other regions of the country. using active, population-based surveillance, we measured the incidence of bacteremic melioidosis in the provinces of sa kaeo (eastern thailand) and nakhon phanom (northeastern thailand) during 2006-2008. the average annual incidence in sa kaeo and nakhon phanom per 100,000 ... | 2011 | 21734135 |
| altered macrophage function is associated with severe burkholderia pseudomallei infection in a murine model of type 2 diabetes. | this study used a murine model of type 2 diabetes (bks.cg-dock7(m) +/+lepr(db)/j mice) to investigate the inflammatory and cellular mechanisms predisposing to burkholderia pseudomallei infection and co-morbid diabetes. homozygous db/db (diabetic) mice developed extreme obesity, dyslipidaemia and glucose intolerance leading to hyperglycaemia and overt type 2 diabetes. compared to their heterozygous db/+ (non-diabetic) littermates, diabetic mice rapidly succumbed to subcutaneous b. pseudomallei in ... | 2011 | 21835260 |
| burkholderia zhejiangensis sp. nov., a novel methyl parathion-degrading bacterium isolated from a wastewater-treating system. | the taxonomic status of a methyl parathion-degrading strain op-1t isolated from a wastewater-treating system was determined. the cells were gram-negative, non-spore-forming, non-motile, and rod-shaped. phylogenetic analysis based on 16s rrna gene sequence indicated that this strain belongs to the genus burkholderia, as it showed highest sequence similarities to burkholderia glathei atcc 29195t (97.4 %), burkholderia sordidicola kctc 12081t (96.5 %) and burkholderia bryophila lmg 23644t (96.3 %). ... | 2011 | 21828021 |
| clinical and economic burden of community-acquired pneumonia amongst adults in the asia-pacific region. | community-acquired pneumonia (cap) is an important cause of mortality and morbidity amongst adults in the asia-pacific region. literature published between 1990 and may 2010 on the clinical and economic burden of cap amongst adults in this region was reviewed. cap is a significant health burden with significant economic impact in this region. chronic obstructive pulmonary disease, cardiovascular disease, diabetes mellitus and advanced age were risk factors for cap. aetiological agents included s ... | 2011 | 21683553 |
| potent antimicrobial peptides with selectivity for bacillus anthracis over human erythrocytes. | in this study, 39 antimicrobial peptides, most with documented low haemolytic activity and potent efficacy against gram-negative and gram-positive bacteria, were evaluated for their haemolytic activity against human red blood cells as well as their antimicrobial activity against escherichia coli, burkholderia thailandensis, bacillus globigii and bacillus anthracis. the majority of the peptides had a minimum inhibitory concentration (mic) of <10++m against b. globigii. however, only eight of thes ... | 2011 | 21741801 |
| effects of trehalose and sorbitol on the activity and structure of pseudomonas cepacia lipase: spectroscopic insight. | the stability of enzymes with no reduction in their catalytic activity still remains a critical issue in industrial applications. naturally occurring osmolytes are commonly used as protein stabilizers. in this study we have investigated the effects of sorbitol and trehalose on the structural stability and activity of pseudomonas cepacia lipase (pcl), using uv-visible, circular dichroism (cd) and fluorescence spectroscopy. surface plasmon resonance (spr) technique was used to trace changes in the ... | 2011 | 21741990 |
| the effects of metabolite molecules produced by drinking water-isolated bacteria on their single and multispecies biofilms. | the elucidation of the mechanisms by which diverse species survive and interact in drinking water (dw) biofilm communities may allow the identification of new biofilm control strategies. the purpose of the present study was to investigate the effects of metabolite molecules produced by bacteria isolated from dw on biofilm formation. six opportunistic bacteria, viz. acinetobacter calcoaceticus, burkholderia cepacia, methylobacterium sp., mycobacterium mucogenicum, sphingomonas capsulata and staph ... | 2011 | 21732713 |
| evaluation and optimisation of bacterial genomic dna extraction for no-culture techniques applied to vinegars. | direct genomic dna extraction from vinegars was set up and suitability for pcr assays performed by pcr/dgge and sequencing of 16s rrna gene. the method was tested on 12 intermediary products of special vinegars, fruit vinegars and condiments produced from different raw materials and procedures. dnas extraction was performed on pellets by chemical, enzymatic, resin mediated methods and their modifications. suitable yield and dna purity were obtained by modification of a method based on the use of ... | 2011 | 21839388 |
| burkholderia pseudomallei: an uncommon cause of bacteraemic pneumonia in a diabetic. | a 55-year-old woman presented with fever, breathlessness and shock. she was diagnosed to have diabetes mellitus (type 2) after admission. blood culture grew burkholderia pseudomallei. the patient responded to intravenous ceftozidime for two weeks and a prolonged course of six months with cotrimoxazole and doxycycline. | 2011 | 21838203 |
| dictyostelium discoideum as a model system for identification of burkholderia pseudomallei virulence factors. | burkholderia pseudomallei is an emerging bacterial pathogen and category b biothreat. human infections with b. pseudomallei (called melioidosis) present as a range of manifestations, including acute septicemia and pneumonia. although melioidosis can be fatal, little is known about the molecular basis of b. pseudomallei pathogenicity, in part because of the lack of simple, genetically tractable eukaryotic models to facilitate en masse identification of virulence determinants or explore host-patho ... | 2011 | 21402765 |
| complete killing of caenorhabditis elegans by burkholderia pseudomallei is dependent on prolonged direct association with the viable pathogen. | burkholderia pseudomallei is the causative agent of melioidosis, a disease of significant morbidity and mortality in both human and animals in endemic areas. much remains to be known about the contributions of genotypic variations within the bacteria and the host, and environmental factors that lead to the manifestation of the clinical symptoms of melioidosis. | 2011 | 21408228 |
| high abundance and role of antifungal bacteria in compost-treated soils in a wildfire area. | compost has been widely used in order to promote vegetation growth in post-harvested and burned soils. the effects on soil microorganisms were scarcely known, so we performed the microbial analyses in a wildfire area of the taebaek mountains, korea, during field surveys from may to september 2007. using culture-dependent and -independent methods, we found that compost used in burned soils influenced a greater impact on soil fungi than bacteria. compost-treated soils contained higher levels of an ... | 2011 | 21409344 |
| distinct roles for nitric oxide in resistant c57bl/6 and susceptible balb/c mice to control burkholderia pseudomallei infection. | burkholderia pseudomallei is the causative agent of melioidosis, an emerging bacterial infectious disease in tropical and subtropical areas. we recently showed that nadph oxidase but not nitric oxide (no) contributes to resistance in innately resistant c57bl/6 mice in a b. pseudomallei respiratory infection model. however, the function of no for resistance was shown to differ among distinct strains of mice and proved also to be stage dependent in various infection models. the present study there ... | 2011 | 21410970 |
| the burkholderia pseudomallei type iii secretion system and bopa are required for evasion of lc3-associated phagocytosis. | burkholderia pseudomallei is the causative agent of melioidosis, a fatal infectious disease endemic in tropical regions worldwide, and especially prevalent in southeast asia and northern australia. this intracellular pathogen can escape from phagosomes into the host cytoplasm, where it replicates and infects adjacent cells. we previously demonstrated that, in response to b. pseudomallei infection of macrophage cell line raw 264.7, a subset of bacteria co-localized with the autophagy marker prote ... | 2011 | 21412437 |
| development of reagents and assays for the detection of pathogenic burkholderia species. | rapid detection of the category b biothreat agents burkholderia pseudomallei and burkholderia mallei in acute infections is critical to ensure that appropriate treatment is administered quickly to reduce an otherwise high probability of mortality (ca. 40% for b. pseudomallei). we are developing assays that can be used in clinical laboratories or security applications for the direct detection of surface-localized and secreted macromolecules produced by these organisms. we present our current medi ... | 2011 | 21413172 |
| quorum sensing inhibitors increase the susceptibility of bacterial biofilms to antibiotics in vitro and in vivo. | although the exact role of quorum sensing (qs) in various stages of biofilm formation, maturation, and dispersal and in biofilm resistance is not entirely clear, the use of qs inhibitors (qsi) has been proposed as a potential antibiofilm strategy. we have investigated whether qsi enhance the susceptibility of bacterial biofilms to treatment with conventional antimicrobial agents. the qsi used in our study target the acyl-homoserine lactone-based qs system present in pseudomonas aeruginosa and bu ... | 2011 | 21422204 |
| the homogentisate and homoprotocatechuate central pathways are involved in 3- and 4-hydroxyphenylacetate degradation by burkholderia xenovorans lb400. | genome characterization of the model pcb-degrading bacterium burkholderia xenovorans lb400 revealed the presence of eleven central pathways for aromatic compounds degradation, among them, the homogentisate and the homoprotocatechuate pathways. however, the functionality of these central pathways in strain lb400 has not been assessed and related peripheral pathways has not been described. | 2011 | 21423751 |
| rice root-associated bacteria: insights into community structures across 10 cultivars. | in this study, the effects of plant genotype, soil type and nutrient use efficiency on the composition of different bacterial communities associated with rice roots were investigated. thus, total bacteria, alpha- and betaproteobacteria, pseudomonas and actinobacteria were studied using pcr, followed by denaturing gradient gel electrophoresis (pcr-dgge). rice genotype determined, to a large extent, the composition of the different bacterial communities across cultivars. several cultivars belongin ... | 2011 | 21426364 |
| impact of chlorophenols on microbiota of an unpolluted acidic soil: microbial resistance and biodegradation. | the impact of 2-monochlorophenol (mcp), 2,4,6-trichlorophenol (tcp) and pentachlorophenol (pcp) on the microbial community of an acidic forest soil was studied under controlled laboratory conditions by spiking microcosms with the pollutants at concentrations ranging from 0.1 to 5000 mg kg(-1) . a decrease in the cumulative respirometric values and changes in the bacterial and fungal community composition were detected at 1000 mg mcp kg(-1) , 100 mg tcp kg(-1) and 100 and 1000 mg pcp kg(-1) . how ... | 2011 | 21426365 |
| marr homologs with urate-binding signature. | bacteria that associate with living hosts require intricate mechanisms to detect and respond to host defenses. part of the early host defense against invading bacteria is the production of reactive oxygen species, and xanthine oxidase is one of the main producers of such agents. the end-product of the enzymatic activity of xanthine oxidase, urate, was previously shown to be the natural ligand for deinococcus radiodurans-encoded hucr and it was shown to attenuate dna binding by agrobacterium tume ... | 2011 | 21432936 |
| biodiesel production in packed-bed reactors using lipase-nanoparticle biocomposite. | the development of appropriate reactors is crucial for the production of biodiesel. in this study, a packed-bed reactor system using lipase-fe(3)o(4) nanoparticle biocomposite catalyst was successfully developed for biodiesel production based on soybean oil methanolysis. emulsification before methanolysis improved the reaction rate. the lipase-nanoparticle biocomposite showed high activity and stability in the single-packed-bed reactor at an optimal flow rate (0.25 ml min(-1)). after 240 h of re ... | 2011 | 21435865 |
| [identification of bacterial contamination in liquid soap for hospital use]. | this study performed a bacteriological analysis of the liquid soap in dispensers that health professionals use for hand washing. this exploratory, cross-sectional study was developed at the hospitalization units of a medium-sized hospital in fortaleza, ceará, brazil. data were collected between may and july 2007. fifty-nine liquid soap dispensers were analyzed, of which 33 contained the following microorganisms: burkholderia cepacia (14), pseudomonas putidas (9), pseudomonas aeruginosa (3), kleb ... | 2011 | 21445502 |
| [bacteriocins and bacteriophages of pathogenic burkholderia]. | detection of bacteriocins and phages in pathogenic bacteria of burkholderia genus and study of their specificity range. | 2011 | 21446163 |
| differences in strategies to combat osmotic stress in burkholderia cenocepacia elucidated by nmr-based metabolic profiling. | to investigate mechanisms of osmotic tolerance in burkholderia cenocepacia, a member of the b. cepacia complex (bcc) of closely related strains, which is of clinical as well as environmental importance. | 2011 | 21446999 |
| vith world melioidosis congress report: celebrating a century of research. | 2011 | 21449838 | |
| in vitro and in vivo studies of monoclonal antibodies with prominent bactericidal activity against burkholderia pseudomallei and burkholderia mallei. | our laboratory has developed more than a hundred mouse monoclonal antibodies (mabs) against burkholderia pseudomallei and burkholderia mallei. these antibodies have been categorized into different groups based on their specificities and the biochemical natures of their target antigens. the current study first examined the bactericidal activities of a number of these mabs by an in vitro opsonic assay. then, the in vivo protective efficacy of selected mabs was evaluated using balb/c mice challenge ... | 2011 | 21450976 |
| burkholderia glumae: next major pathogen of rice? | burkholderia glumae causes bacterial panicle blight of rice, which is an increasingly important disease problem in global rice production. toxoflavin and lipase are known to be major virulence factors of this pathogen, and their production is dependent on the tofi/tofr quorum-sensing system, which is mediated by n-octanoyl homoserine lactone. flagellar biogenesis and a type iii secretion system are also required for full virulence of b. glumae. bacterial panicle blight is thought to be caused by ... | 2011 | 21453428 |
| ligand binding induces an ammonia channel in 2-amino-2-desoxyisochorismate (adic) synthase phze. | phze utilizes chorismate and glutamine to synthesize 2-amino-2-desoxyisochorismate (adic) in the first step of phenazine biosynthesis. the phze monomer contains both a chorismate-converting menaquinone, siderophore, tryptophan biosynthesis (mst) and a type 1 glutamine amidotransferase (gatase1) domain connected by a 45-residue linker. we present here the crystal structure of phze from burkholderia lata 383 in a ligand-free open and ligand-bound closed conformation at 2.9 and 2.1 å resolution, re ... | 2011 | 21454481 |
| a psychrotrophic burkholderia cepacia strain isolated from refrigerated raw milk showing proteolytic activity and adhesion to stainless steel. | the proteolytic activity of a psychrotrophic strain of burkholderia cepacia isolated from refrigerated raw milk was characterized. bur. cepacia produced proteolytic activity during growth at refrigeration temperature, with maximum activity at ph 6-7. the enzyme showed relative thermal stability in the range 40-50°c during 25 min, and maintained 80% its initial activity at 76°c/30 s. milk coagulation assay showed that the crude protease from bur. cepacia caused coagulation from day 2 for skimmed ... | 2011 | 21457588 |
| a chemically modified lipase preparation for catalyzing the transesterification reaction in even highly polar organic solvents. | acylation of pseudomonas cepacia lipase with pyromellitic dianhydride to modify 72% of total amino groups was carried out. different organic solvents were screened for precipitation of modified lipase. it was found that 1,2-dimethoxyethane was the best precipitant which precipitated 97% protein and complete activity. pcmc (protein coated microcrystals), clpcmc (crosslinked protein coated microcrystals), epros (enzyme precipitated and rinsed with organic solvents) and ph tuned preparations of mod ... | 2011 | 21463943 |
| complete genome sequence of burkholderia gladioli bsr3. | we report the complete genome sequence of burkholderia gladioli bsr3, isolated from a diseased rice sheath in south korea. | 2011 | 21478339 |
| burkholderia cepacia endophthalmitis: clinico-microbiologic profile and outcomes. | background:: to report the clinical presentations, antibiotic sensitivities, and outcomes of burkholderia cepacia endophthalmitis. methods:: retrospective, consecutive, noncomparative, interventional case series. we reviewed case records of culture-proven b. cepacia endophthalmitis from the endophthalmitis registry between january 2003 and december 2008. data collected included the cause of endophthalmitis, time of presentation, presenting visual acuity, initial surgical procedures performed, cl ... | 2011 | 21478807 |
| comparative in vitro susceptibility of burkholderia pseudomallei to doripenem, ertapenem, tigecycline and moxifloxacin. | burkholderia pseudomallei, the causative agent of melioidosis, continues to present therapeutic challenges in endemic areas. a number of clinical issues have prompted consideration of alternative antimicrobial therapies. these include stability in 24-h infusion pumps, broad-spectrum coverage in the empirical treatment of community-acquired pneumonia, cost, the need for effective oral agents and rare reports of emerging resistance. this study aimed to examine the in vitro susceptibility of b. pse ... | 2011 | 21481571 |
| subsurface examination of a foliar biofilm using scanning electron- and focused-ion-beam microscopy. | the dual beam scanning electron microscope, equipped with both a focused ion- and scanning electron-beam (fib sem) is a novel tool for the exploration of the subsurface structure of biological tissues. the fib can remove a predetermined amount of material from a selected site to allow for subsurface exploration and when coupled with sem or scanning ion-beam microscopy (sim) could be suitable to examine the subsurface structure of bacterial biofilms on the leaf surface. the suitability of chemica ... | 2011 | 21482121 |
| molecular phylogeny of burkholderia pseudomallei from a remote region of papua new guinea. | the island of new guinea is located midway between the world's two major melioidosis endemic regions of australia and southeast asia. previous studies in papua new guinea have demonstrated autochthonous melioidosis in balimo, western province. in contrast to other regions of endemicity, isolates recovered from both environmental and clinical sources demonstrate narrow genetic diversity over large spatial and temporal scales. | 2011 | 21483841 |
| the airway microbiome in cystic fibrosis and implications for treatment. | lung disease in cystic fibrosis (cf) results from chronic airway infection and inflammation leading to progressive bronchiectasis and respiratory failure. bacterial pathogens, including pseudomonas aeruginosa, staphylococcus aureus, and burkholderia cepacia, are known contributors. recent studies using culture-independent molecular techniques and anaerobic cultures have broadened our view of cf airway bacterial communities. | 2011 | 21494150 |
| elucidating the reaction mechanism of the benzoate oxidation pathway encoded aldehyde dehydrogenase from burkholderia xenovorans lb400. | oxidation of cis-3,4-dehydroadipyl-coa semialdehyde to cis-3,4-dehydroadipyl-coa by the aldehyde dehydrogenase, aldh(c) (ec.1.2.1.77), is an essential step in the metabolism of benzoate in burkholderia xenovorans lb400. in a previous study, we established a structural blueprint for this novel group of aldh enzymes. here, we build significantly on this initial work and propose a detailed reaction mechanism for aldh(c) based on comprehensive structural and functional investigations of active site ... | 2011 | 21495107 |
| disseminated burkholderia gladioli infection in a lung transplant recipient with underlying hypocomplementemic urticarial vasculitis. | g.r. thompson iii, b.l. wickes, m.l. herrera, t.c. haman, j.s. lewis ii, j.h. jorgensen. disseminated burkholderia gladioli infection in a lung transplant recipient with underlying hypocomplementemic urticarial vasculitis. transpl infect dis 2011. all rights reserved abstract: burkholderia gladioli is difficult to definitively identify within the laboratory using phenotypic testing alone. we describe a case of recurrent b. gladioli infection in a lung transplant recipient with underlying hypocom ... | 2011 | 21504528 |
| phenylalanine induces burkholderia cenocepacia phenylacetic acid catabolism through degradation to phenylacetyl-coa in synthetic cystic fibrosis sputum medium. | synthetic cystic fibrosis sputum medium (scfm) is rich in amino acids and supports robust growth of burkholderia cenocepacia, a member of the burkholderia cepacia complex (bcc). previous work demonstrated that b. cenocepacia phenylacetic acid (pa) catabolic genes are up-regulated during growth in scfm and are required for full virulence in a caenorhabditis elegans host model. in this work, we investigated the role of phenylalanine, one of the aromatic amino acids present in scfm, as an inducer o ... | 2011 | 21511027 |
| [effect of probiotic bacteria on survival and growth of cortez oyster larvae, crassostrea corteziensis (bivalvia: ostreidae)]. | disease control problems have major constraints in aquaculture production, and the use of probiotics in larviculture is a valid alternative to antibiotics. this study analyzed the effect of probiotic bacteria on survival and final size of cortez oyster larvae crassostrea corteziensis. two different probiotic concentrations were evaluated, 1 x 10(4) and 1 x 10(5) cfu/ml of lactic acid bacteria (strain ns61) isolated from nodipecten subnodosus, and bacilli isolated from the white leg shrimp, litop ... | 2011 | 21516645 |
| genomic analysis of the phenylacetyl-coa pathway in burkholderia xenovorans lb400. | the phenylacetyl-coa (paa) catabolic pathway and genome-wide gene expression responses to phenylacetate catabolism were studied in the polychlorinated biphenyl (pcb)-degrading strain burkholderia xenovorans lb400. microarray and rt-qpcr analyses identified three non-contiguous chromosomal clusters of genes that are predicted to encode a complete paa pathway that were induced up to 40-fold during growth of lb400 on phenylacetate: paaghijkr, paaanebdf, and paac. comparison of the available genome ... | 2011 | 21519854 |
| mycotic aneurysm caused by burkholderia pseudomallei: report of a brazilian strain genetically related to thai strains. | clin microbiol infect 2011; 17: 719-721 abstract: melioidosis, a severe infectious disease caused by burkholderia pseudomallei that is prevalent in southeast asia and northern australia, has been sporadically reported in brazil since 2003. we report a case of aortic aneurysm with blood culture positive for b. pseudomallei. the phylogenetic analysis of 16s ribosomal dna showed this isolate to be evolutionarily grouped with the mshr346 strains from thailand. | 2011 | 21521412 |
| identification of unknown ocular pathogens in clinically suspected eye infections using ribosomal rna gene sequence analysis. | clin microbiol infect 2011; 17: 776-779 abstract: dna sequence-based identification of pathogens from ocular samples of patients with clinically suspected eye infections was accomplished using 16s and internal transcribed spacer (its) ribosomal rna gene sequence analysis. pcr was positive for 24 of 99 samples tested. both culture and 16s rdna sequence analysis identified pseudomonas aeruginosa, streptococci and enterobacteriaceae. isolates misidentified as burkholderia cepacia by biochemical tes ... | 2011 | 21521414 |
| metagenomic analysis of the 1-aminocyclopropane-1-carboxylate deaminase gene (acds) operon of an uncultured bacterial endophyte colonizing solanum tuberosum l. | deamination of the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (acc) is a key plant-beneficial trait found in many plant growth-promoting bacteria. in this study, we analysed acc deaminase genes (acds) of bacterial endophytes colonizing field-grown potato plants. pcr analysis revealed the presence of two types of acds genes, the dominant one showing high homology to an acds gene derived from pseudomonas fluorescens. construction, functional screening and sequence analysis of metagen ... | 2011 | 21523387 |