Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| the impact of alteplase on pulmonary graft function in donation after circulatory death - an experimental study. | lung transplantation is hampered by the lack of organs resulting in deaths on the waiting list. the usage of donation after circulatory death (dcd) lungs would dramatically increase donor availability. the most optimal organ preservation method, and the need for antithrombotic and fibrinolytic treatment to prevent thrombosis in the donor lungs is currently on debate. the present study investigated, in a simulated clinical dcd situation, whether the addition of alteplase in the flush-perfusion so ... | 2017 | 28839932 |
| the epidemiology of african swine fever in "nonendemic" regions of zambia (1989-2015): implications for disease prevention and control. | african swine fever (asf) is a highly contagious and deadly viral hemorrhagic disease of swine. in zambia, asf was first reported in 1912 in eastern province and is currently believed to be endemic in that province only. strict quarantine measures implemented at the luangwa river bridge, the only surface outlet from eastern province, appeared to be successful in restricting the disease. however, in 1989, an outbreak occurred for the first time outside the endemic province. sporadic outbreaks hav ... | 2017 | 28832525 |
| multiple stent delivery system multi-loc, a new technology for spot-stenting of the femoropopliteal artery - proof of concept study in a preclinical large animal model. | a new stent system was studied in a porcine model to evaluate its feasibility for spot-stenting of the femoropopliteal artery. | 2017 | 28825354 |
| convergent and divergent genetic changes in the genome of chinese and european pigs. | since 10,000 bc, continuous human selection has led to intense genetic and phenotypic changes in pig (sus scrofa) domestication. through whole genome analysis of 257 individuals, we demonstrated artificial unidirectional and bidirectional selection as the primary force to shape the convergent and divergent changes between chinese domestic pigs (chd) and european domestic pigs (eud). we identified 31 genes in unidirectional selection regions that might be related to fundamental domestication requ ... | 2017 | 28819228 |
| effect of ha330 resin-directed hemoadsorption on a porcine acute respiratory distress syndrome model. | blood purification is an emerging approach to dampening the cytokine storm. this study aims to assess the efficacy of ha330 resin-directed hemoadsorption (ha) on endotoxin-induced porcine acute respiratory distress syndrome (ards) model. | 2017 | 28808944 |
| origin and dispersal of early domestic pigs in northern china. | it is widely accepted that modern pigs were domesticated independently at least twice, and chinese native pigs are deemed as direct descendants of the first domesticated pigs in the corresponding domestication centers. by analyzing mitochondrial dna sequences of an extensive sample set spanning 10,000 years, we find that the earliest pigs from the middle yellow river region already carried the maternal lineages that are dominant in both younger archaeological populations and modern chinese pigs. ... | 2017 | 28798357 |
| quantitative approach for the risk assessment of african swine fever and classical swine fever introduction into the united states through legal imports of pigs and swine products. | the us livestock safety strongly depends on its capacity to prevent the introduction of transboundary animal diseases (tads). therefore, accurate and updated information on the location and origin of those potential tads risks is essential, so preventive measures as market restrictions can be put on place. the objective of the present study was to evaluate the current risk of african swine fever (asf) and classical swine fever (csf) introduction into the us through the legal importations of live ... | 2017 | 28797058 |
| a test for paedomorphism in domestic pig cranial morphology. | domestic animals are often described as paedomorphic, meaning that they retain juvenile characteristics into adulthood. through a three-dimensional landmark-based geometric morphometric analysis of cranial morphology at three growth stages, we demonstrate that wild boar (n= 138) and domestic pigs (n= 106) (sus scrofa) follow distinct ontogenetic trajectories. with the exception of the size ratio between facial and neurocranial regions, paedomorphism does not appear to be the primary pattern desc ... | 2017 | 28794276 |
| preclinical efficacy and safety of kcnh2-g628s gene therapy for postoperative atrial fibrillation. | postoperative atrial fibrillation (poaf) is the most common complication occurring after cardiac surgery. multiple studies have shown significantly increased risks of stroke, myocardial infarction, and death associated with poaf. current prophylaxis strategies are inadequate to eliminate this problem. we examined the preclinical efficacy and safety of kcnh2-g628s gene transfer to prevent poaf. | 2017 | 28676183 |
| immunoregulatory effects of multipotent adult progenitor cells in a porcine ex vivo lung perfusion model. | primary graft dysfunction (pgd) is considered to be the end result of an inflammatory response targeting the new lung allograft after transplant. previous research has indicated that mapc cell therapy might attenuate this injury by its paracrine effects on the pro-/anti-inflammatory balance. this study aims to investigate the immunoregulatory capacities of mapc cells in pgd when administered in the airways. | 2017 | 28676074 |
| a high sucrose and high fat diet induced the development of insulin resistance in the skeletal muscle of bama miniature pigs through the akt/glut4 pathway. | a high sucrose and high fat (hshf) diet induces insulin resistance (ir) and increased susceptibility to type 2 diabetes mellitus (t2dm), but the underlying mechanisms are poorly characterized. this study aimed to investigate the molecular mechanisms by which the hshf diet impairs insulin sensitivity in bama miniature pigs (sus scrofa domesticus). twelve bama miniature pigs were randomly assigned to the control diet (cd) group (n=6) or the hshf group (n=6) for 6 months. biochemical parameters wer ... | 2017 | 28674285 |
| physiological mechanisms through which heat stress compromises reproduction in pigs. | seasonal variations in environmental temperatures impose added stress on domestic species bred for economically important production traits. these heat-mediated stressors vary on a seasonal, daily, or spatial scale, and negatively impact behavior and reduce feed intake and growth rate, which inevitably lead to reduced herd productivity. the seasonal infertility observed in domestic swine is primarily characterized by depressed reproductive performance, which manifests as delayed puberty onset, r ... | 2017 | 28667793 |
| endoluminal nd:yag laser application in ex vivo biliary porcine tissue. | adequate biliary drainage with endoscopic or percutaneous placement of self-expandable metal stents represents the goal of palliation in patients with inoperable malignant obstruction of the biliary tree. as an adjunct to stenting, various tissue ablation treatments have been proposed with conflicting results. the aim of this study was to test the effect on biliary tissue of a new ablation technique based on nd:yag laser light delivery. the study was conducted on ex vivo specimens of 18 healthy ... | 2017 | 28667507 |
| reliability of transcardiopulmonary thermodilution cardiac output measurement in experimental aortic valve insufficiency. | monitoring cardiac output (co) is important to optimize hemodynamic function in critically ill patients. the prevalence of aortic valve insufficiency (ai) is rising in the aging population. however, reliability of co monitoring techniques in ai is unknown. the aim of this study was to investigate the impact of ai on accuracy, precision, and trending ability of transcardiopulmonary thermodilution-derived cotcptd in comparison with pulmonary artery catheter thermodilution copac. | 2017 | 29049339 |
| population admixture in chinese and european sus scrofa. | relationships between different populations were investigated using porcine 60 k data from 1,135 domestic pigs and wild boars across europe and china. the results indicate that most european breeds have been introgressed with chinese ancestry, but the extent of introgression varies considerably among breeds. moreover, the main source of this introgression is pigs from south china, closely related to bamaxiang and dongshan pigs. contributions from east and central chinese pig breeds are also dete ... | 2017 | 29030577 |
| simultaneous profiles of sulfonated androgens, sulfonated estrogens and sulfonated progestogens in postpubertal boars (sus scrofa domestica) measured by lc-ms/ms. | sulfonated steroids (s-st) have been usually regarded as inactive metabolites but are progressively considered as precursors for the intra-tissue formation of bioactive steroids. moreover, independent effects without preceding removal of the sulfate group have been observed. we use the porcine testicular-epididymal compartment as a model to investigate the still largely unknown s-st physiology as the boar exhibits an intriguingly broad s-st spectrum predominantly originating from the testis. the ... | 2017 | 29030154 |
| biocompatibility and osseointegration of titanium implants with a silver-doped polysiloxane coating: an in vivo pig model. | to test the antimicrobial properties, surface topography, reaction of surrounding tissue (biocompatibility), and osseointegration of ultrathin implant surfaces containing polysiloxane and nanoscaled silver particles. | 2017 | 29028852 |
| why is african swine fever still present in sardinia? | african swine fever (asf) is an infectious disease of swine that has been present in sardinia since 1978. soon after introduction of the disease, several control and eradication programmes were established with limited success. some researchers attributed the persistence of the disease in central and eastern areas to certain socio-economic factors, the existence of some local and traditional farming practices (i.e., unregistered free-ranging pigs known as brado animals) and the high density of w ... | 2017 | 29027378 |
| characterization of the african swine fever virus decapping enzyme during infection. | african swine fever virus (asfv) infection is characterized by a progressive decrease in cellular protein synthesis with a concomitant increase in viral protein synthesis, though the mechanism by which the virus achieves this is still unknown. decrease of cellular mrna is observed during asfv infection, suggesting that inhibition of cellular proteins is due to an active mrna degradation process. asfv carries a gene (ba71v d250r/malawi g5r) that encodes a decapping protein (asfv-dp) that has a nu ... | 2017 | 29021398 |
| a preclinical evaluation comparing the performance of a novel 19-g flexible needle to a commercially available 22-g ebus-tbna sampling needle. | needle samples may not provide sufficient diagnostic material for the assessment of mediastinal lymph nodes. | 2017 | 29020679 |
| signatures of selection and interspecies introgression in the genome of chinese domestic pigs. | chinese domestic pigs have experienced strong artificial selection for thousands of years. however, the molecular mechanisms underlying the selection-causing phenotypic changes in chinese domestic pigs are still largely unknown. here we used whole-genome resequencing data of 54 pigs from 9 chinese diverse breeds and 16 wild boars from 7 localities across china to identify genes that show evidence of positive selection in the process of domestication. a total of 14 candidate domestication regions ... | 2017 | 29016799 |
| improved method for the establishment of an in vitro blood-brain barrier model based on porcine brain endothelial cells. | the aim of this protocol presents an optimized procedure for the purification and cultivation of pbecs and to establish in vitro blood-brain barrier (bbb) models based on pbecs in mono-culture (mc), mc with astrocyte-conditioned medium (acm), and non-contact co-culture (ncc) with astrocytes of porcine or rat origin. pbecs were isolated and cultured from fragments of capillaries from the brain cortices of domestic pigs 5-6 months old. these fragments were purified by careful removal of meninges, ... | 2017 | 28994773 |
| ex vivo treatment with inhaled n-acetylcysteine in porcine lung transplantation. | we have shown the beneficial effects of n-acetylcysteine (nac) on posttransplant lung function, when both donor and recipient were pretreated intravenously. however, systemic treatment of multiorgan donors may not be clinically relevant. thus, we hypothesized that ex vivo treatment of donors with nebulized nac would be adequate to prevent from ischemia-reperfusion injury after lung transplantation. | 2017 | 28985871 |
| molecular study of porcine circovirus type 2 in wild boars and domestic pigs in uruguay from 2010 to 2014: predominance of recombinant circulating strains. | porcine circovirus type 2 (pcv2) is a worldwide distributed pathogen and one of the most economically relevant swine infections. four genotypes have been recognized and it is well known that pcv2a, pcv2b and pcv2d have a global distribution. however, the information about recombinant strains circulation and their influence in driving pcv2 evolution is a poorly studied area. in uruguay, pcv2 associated symptoms began to be frequently observed in pigs from different farms since 2010. the main purp ... | 2017 | 28964894 |
| complete genome sequences and phylogenetic analysis of encephalomyocarditis virus strains isolated from pigs and rats origin. | in order to evaluate the genetic variability of encephalomyocarditis virus (emcv), the whole genomes of six emcv field isolates originating from pigs and rats origin in different regions of central china, were phylogenetically and comparatively analyzed. phylogenetic analysis of whole genome sequences, open reading frame (orf), capsid coding region (ccr) and vp3/vp1 using neighbor-joining analysis revealed that these isolates belonged to lineage 1. nucleotide sequences of six isolates showed mor ... | 2017 | 28964856 |
| detection of toxoplasma gondii in naturally infected domestic pigs in northern serbia. | insufficiently cooked pork is considered as an important source of human infection with toxoplasma gondii. the aim of our study was to investigate the presence of t. gondii in pigs intended for human consumption from northern serbia. blood and diaphragm samples were collected from 182 naturally infected market-weight pigs, originating from both commercial farms and smallholdings. sera were examined using modified agglutination test (mat), and diaphragms from seropositive, as well as from some ma ... | 2017 | 28956155 |
| interventional magnetic resonance imaging guided carotid embolectomy using a novel resonant marker catheter: demonstration of preclinical feasibility. | to assess the visualization and efficacy of a wireless resonant circuit (wrc) catheter system for carotid artery occlusion and embolectomy under real-time mri guidance in vivo, and to compare mr imaging modality with x-ray for analysis of qualitative physiological measures of blood flow at baseline and after embolectomy. the wrc catheter system was constructed using a mr compatible peek fiber braided catheter (penumbra, inc, alameda, ca) with a single insulated longitudinal copper loop soldered ... | 2017 | 28948399 |
| safety and efficacy of new biodegradable polymer-based sirolimus-eluting stents in a preclinical model. | new drug-eluting stents (des) designed to overcome the limitations of existing devices should initially be tested in preclinical studies. our objective was to analyze the safety and efficacy of new biodegradable polymer-based des compared with bare-metal stents (bms) and commercially available des in a model of normal porcine coronary arteries. | 2017 | 28647315 |
| annotation of the domestic pig genome by quantitative proteogenomics. | the pig is one of the earliest domesticated animals in the history of human civilization and represents one of the most important livestock animals. the recent sequencing of the sus scrofa genome was a major step toward the comprehensive understanding of porcine biology, evolution, and its utility as a promising large animal model for biomedical and xenotransplantation research. however, the functional and structural annotation of the sus scrofa genome is far from complete. here, we present mass ... | 2017 | 28625053 |
| protein requirements of collared peccary (pecari tajacu). | a nitrogen (n) balance digestion trial was conducted to determine the protein requirement of collared peccaries (pecari tajacu). in a 4 × 4 latin square design, four captive adult male peccaries were fed four isoenergy diets containing four different levels of n (11.7, 16.3, 22.8, and 26.7 g n/kg of dry matter-dm). after 15 days of adaptation, a total collection of feces and urine was carried out for five consecutive days. regression analyses between n intake and n in feces and urine allowed to ... | 2017 | 28623602 |
| adrenergic regulation of cytoplasmic structures related to secretory processes in pig pinealocytes-an ultrastructural, quantitative study. | two structures, considered as secretory in nature, are present in the pinealocytes in of the domestic pig show the presence of two structures, which are considered as secretory in nature - the dense core vesicles (dcv) and the membrane bounded (dense) bodies (mbb). the latter are extremely numerous in pig pinealocytes (they occupy 6-20% of the cytoplasm), and the number of mbb changes under different physiological and experimental conditions. norepinephrine is the main neurotransmitter that regu ... | 2017 | 28622599 |
| epidemiological studies on trichinellosis in pigs (sus scofa) in india. | the present study was conducted to determine the prevalence of trichinellosis in domestic pigs (sus scrofa)in india. a total of 319 tissue and 279 serum samples were collected from 279 slaughter pigs. the samples were collected from small slaughter shops located in assam, chandigarh (union territory), punjab and uttarakhand states of the country. the presence oftrichinellaspp. antibodies in serum was determined using a commercially available enzyme-linked immunosorbent assay (elisa) kit and tiss ... | 2017 | 28615865 |
| genome-wide dynamics of nascent noncoding rna transcription in porcine heart after myocardial infarction. | microarrays and rna sequencing are widely used to profile transcriptome remodeling during myocardial ischemia. however, the steady-state rna analysis lacks in sensitivity to detect all noncoding rna species and does not provide separation between transcriptional and post-transcriptional regulations. here, we provide the first comprehensive analysis of nascent rna profiles of mrnas, primary micro-rnas, long noncoding rnas, and enhancer rnas in a large animal model of acute infarction. | 2017 | 28611032 |
| repeated subretinal surgery and removal of subretinal decalin is well tolerated - evidence from a porcine model. | subretinal perfluorocarbon liquid (pfcl) is a serious complication that can occur after retinal detachment repair. it is possible to remove the pfcl surgically, but retinal damage related to the procedure is unknown. also, increasing interest in subretinal treatment makes it relevant to examine the functional and morphological consequences of repeated subretinal manipulation. we hypothesized that pfcl in a porcine model can be injected in the subretinal space and removed with minimal effect on r ... | 2017 | 28608271 |
| periosteal elevation induces supracortical peri-implant bone formation. | the aim of the study was to evaluate the possibility of supracortical peri-implant bone formation after periosteal elevation. | 2017 | 28606438 |
| novel porcine epidemic diarrhea virus (pedv) variants with large deletions in the spike (s) gene coexist with pedv strains possessing an intact s gene in domestic pigs in japan: a new disease situation. | since late 2013, after an absence of seven years, outbreaks of porcine epidemic diarrhea virus (pedv) infection have reemerged and swept rapidly across japan, resulting in significant economic losses. in this study, we report the emergence, mixed infection, and genetic characterization of 15 novel field pedv variants with large genomic deletions. the sizes of deletion varied between 582 nt (194 aa) and 648 nt (216 aa) at positions 28-714 (10-238) on the s gene (protein). among 17 pedv samples is ... | 2017 | 28095455 |
| first identification of porcine parvovirus 6 in poland. | porcine parvovirus type 1 is a major causative agent of swine reproductive failure. during the past decade, several new parvoviruses have been discovered in pigs. porcine parvovirus type 6 (ppv6), recently identified, has been reported in pigs in china and in the usa while the ppv6 status in the european pig population remains undetermined. in the present study, ppv6 dna was identified in serum samples collected from domestic pigs in poland. in investigated herds, the prevalence of ppv6 was 14.9 ... | 2017 | 27590228 |
| evaluation of five serologic assays for bovine tuberculosis surveillance in domestic free-range pigs from southern spain. | in countries where bovine tuberculosis (btb) is still prevalent the contact among different animal species in extensive systems contributes to the circulation of mycobacterium bovis (m. bovis) and other members of the mycobacterium tuberculosis complex (mtc). thus, free-range pigs can develop subclinical infections and may contribute to disease spread to bovine and wildlife. serodiagnosis has been proposed as a screening tool for detecting infected pig herds; however, the value of this method to ... | 2017 | 28089289 |
| influenza a virus infection in japanese wild boars (sus scrofa leucomystax). | serum samples were collected from 385 wild boars between 2010 and 2013 to examine the seroprevalence of influenza a virus (iav) in japan. antibodies against iav were identified using a commercial kit in 13 wild boars (3.4%). to identify the serotypes, positive sera were examined by virus-neutralization test using representative serotypes and strains. three wild boars in yamaguchi and four in tochigi showed the highest antibody titers against the pandemic h1n1 2009 virus and classical swine h1n1 ... | 2017 | 28321029 |
| autochthonous infection with hepatitis e virus related to subtype 3a, france: a case report. | hepatitis e virus (hev) recently emerged in europe as a cause of autochthonous acute hepatitis and a porcine zoonosis. european autochthonous cases almost exclusively involved viruses of genotype 3, subtype 3a being only recently reported in france, from farm pigs. we report an autochthonous human infection with a hev related to subtype 3a in southeastern france. a 55-year-old human immunodeficiency virus-infected man presented liver cytolysis in june 2014. hev rna was detected in serum and thre ... | 2017 | 27049499 |
| risk analysis and occurrence of hepatitis e virus (hev) in domestic swine in northeast brazil. | anti-hev antibodies were detected in animals from abattoir and in farms from northeast brazil. our results suggest that hev is highly disseminated in the swine population and might present a great risk to animal handlers and for consumption of raw or undercooked meat and meat products in northeast brazil. | 2017 | 28349492 |
| autochthonous sporadic acute hepatitis e caused by two distinct subgenotype 3b hepatitis e virus strains with only 90% nucleotide identity. | hepatitis e, which is caused by hepatitis e virus (hev), is a public health concern in japan, where the zoonotic food-borne transmission of hev from domestic pigs and wild boars plays an important role. a 44-year-old japanese man with autochthonous sporadic acute hepatitis e was admitted with general fatigue and moderate liver dysfunction. in the present study, two distinct hev strains were recovered from the patient, who had consumed the raw or undercooked pig liver and intestine two or three t ... | 2017 | 28205112 |
| prevalence and determinants of cryptosporidium infection in an underdeveloped rural region of southwestern china. | few studies have focused on the epidemiology of cryptosporidium in resource-challenged settings in china. we report a community-based cross-sectional study to investigate the prevalence of cryptosporidium infection and its risk factors and associations with hepatitis b virus (hbv), hepatitis c virus (hcv), and human immunodeficiency virus (hiv) infections. the prevalence of cryptosporidium infection was 12.6% (95% confidence interval = 11.0-14.3). individuals living in households with ≥ 5 family ... | 2017 | 28093538 |
| chlamydial antibiotic resistance and treatment failure in veterinary and human medicine. | the chlamydiaceae are widespread pathogens of both humans and animals. chlamydia trachomatis infection causes blinding trachoma and reproductive complications in humans. chlamydia pneumoniae causes human respiratory tract infections and atypical pneumonia. chlamydia suis infection is associated with conjunctivitis, diarrhea, and failure to gain weight in domestic swine. chlamydial infections in humans and domesticated animals are generally controlled by antibiotic treatment-particularly macrolid ... | 2017 | 27218014 |
| visual detection and differentiation of classic swine fever virus strains using nucleic acid sequence-based amplification (nasba) and g-quadruplex dnazyme assay. | the split g-quadruplex dnazyme has emerged as a valuable tool for visual dna detection. here, we successfully integrated colorimetric split g-quadruplex dnazyme assay with nucleic acid sequence-based amplification to generate a novel detection approach, allowing visual and rapid detection for the rna of shimen and hclv strains of classic swine fever virus (csfv). csfv is a rna virus that causes a highly contagious disease in domestic pigs and wild boar. with this method, we were able to detect a ... | 2017 | 28287135 |
| quasispecies composition and diversity do not reveal any predictors for chronic classical swine fever virus infection. | classical swine fever (csf) can run acute, chronic, and prenatal courses in both domestic pigs and wild boar. although chronic infections are rare events, their epidemiological impact is very high due to the long-term shedding of virus. so far, little is known about the factors that influence disease course and outcome from either the host or virus's perspective. to elucidate the viral determinants, we analyzed the role of the viral populations for the development of chronic csf virus (csfv) inf ... | 2017 | 27885563 |
| experimental evaluation of faecal escherichia coli and hepatitis e virus as biological indicators of contacts between domestic pigs and eurasian wild boar. | domestic pigs and eurasian wild boar (sus scrofa) share several important viral and bacterial pathogens. therefore, direct and indirect contacts between domestic pigs and wild boar present a risk of pathogen spillover and can lead to long-term perpetuation of infection. biological indicators could be a powerful tool to understand and characterize contacts between wild boar and domestic pigs. here, faecal escherichia coli and hepatitis e virus (hev) were explored as potential biological indicator ... | 2017 | 26190581 |
| recombinant expression of porcine spermadhesin awn and its phospholipid interaction: indication for a novel lipid binding property. | awn is a porcine (sus scrofa domestica) seminal plasma protein and has been linked to a variety of processes related to fertilization. to acquire the protein in sufficient amount and purity for functional studies, we established its recombinant expression in e. coli and a three-step purification protocol based on different chromatographies. the test for awn-phospholipid interaction revealed phosphatidic acid and cardiolipin as potential binding partners. as phosphatidic acid is surmised to play ... | 2017 | 28326614 |
| experimental infection of domestic pigs with african swine fever virus lithuania 2014 genotype ii field isolate. | an experimental infection was conducted to evaluate horizontal transmission, clinical, virological and humoral response induced in domestic pigs infected with african swine fever (asf) genotype ii virus circulating in 2014 into the european union (eu). ten naive pigs were placed in contact with eight pigs experimentally inoculated with the lithuanian lt14/1490 asf virus (asfv) responsible for the first asf case detected in wild boar in lithuania in january 2014. clinical examination and rectal t ... | 2017 | 25808027 |
| the assessment of african swine fever virus risk to belgium early 2014, using the quick and semiquantitative pandora screening protocol. | a risk assessment was organized during the early eu asf outbreaks of early 2014 (february-april) and performed in cooperation with 15 belgian and european experts on asfv and its epidemiology in pigs/wild boar. african swine fever (asf) is considered as one of the most dangerous infectious pig diseases, causing many outbreaks. since the end of 2013 - early 2014, several outbreaks within the european union (lithuania, poland, estonia and latvia) were reported to oie, which prompted several risk a ... | 2017 | 25939453 |
| the domestic pig as a potential model for borrelia skin infection. | the skin lesion erythema migrans is a characteristic early manifestation of lyme borreliosis in humans. however, the pathomechanisms leading to development of this erythema are not fully understood. models that mimic human skin would enhance research in this field. human and porcine skin structures strongly resemble each other. therefore, we attempted to induce erythema migrans lesions in experimental borrelia burgdorferi sensu lato infection in the skin of domestic pigs. the formation of erythe ... | 2017 | 27986402 |
| boar hunting and brucellosis caused by brucella suis. | brucellosis remains as a significant public health concern in many areas where the infection persists in domestic hosts (i.e. goats, cattle, and domestic swine) with subsequent risk of transmission to human populations. brucellosis caused by b. suis remains an important threat to human populations in many countries exposed to domestic and feral swine. in the u.s., swine brucellosis has been under control for many years. meanwhile, it is a widespread infection among feral swine, particularly in t ... | 2017 | 28300645 |
| dna-binding properties of the african swine fever virus pa104r, a histone-like protein involved in viral replication and transcription. | african swine fever virus (asfv) codes for a putative histone-like protein (pa104r) with extensive sequence homology to bacterial proteins that are implicated in genome replication and packaging. functional characterization of purified recombinant pa104r revealed that it binds to ssdna and dsdna over a wide range of temperatures, ph values, salt concentrations and in an atp-independent manner, with an estimated binding site size of about 14-16 nucleotides. using site-directed mutagenesis, the ar ... | 2017 | 28381576 |
| co-circulation of multiple genotypes of african swine fever viruses among domestic pigs in zambia (2013-2015). | during 2013-2015, several and severe outbreaks of african swine fever (asf) affected domestic pigs in six provinces of zambia. genetic characterization of asf viruses (asfvs) using standardized genotyping procedures revealed that genotypes i, ii and xiv were associated with these outbreaks. molecular and epidemiological data suggest that genotype ii asfv (georgia 2007/1-like) detected in northern province of zambia may have been introduced from neighbouring tanzania. also, a genotype ii virus de ... | 2017 | 28299893 |
| understanding african swine fever infection dynamics in sardinia using a spatially explicit transmission model in domestic pig farms. | african swine fever virus (asfv) has been endemic in sardinia since 1978, resulting in severe losses for local pig producers and creating important problems for the island's veterinary authorities. this study used a spatially explicit stochastic transmission model followed by two regression models to investigate the dynamics of asfv spread amongst domestic pig farms, to identify geographic areas at highest risk and determine the role of different susceptible pig populations (registered domestic ... | 2017 | 28296281 |
| genetic assessment of african swine fever isolates involved in outbreaks in the democratic republic of congo between 2005 and 2012 reveals co-circulation of p72 genotypes i, ix and xiv, including 19 variants. | african swine fever (asf) is a devastating disease of domestic pigs. it is a socioeconomically important disease, initially described from kenya, but subsequently reported in most sub-saharan countries. asf spread to europe, south america and the caribbean through multiple introductions which were initially eradicated-except for sardinia-followed by re‑introduction into europe in 2007. in this study of asf within the democratic republic of the congo, 62 domestic pig samples, collected between 20 ... | 2017 | 28218698 |
| variations in clinical presentation and anatomical distribution of gross lesions of african swine fever in domestic pigs in the southern highlands of tanzania: a field experience. | african swine fever is a contagious viral disease responsible for up to 100% mortality among domestic pigs. a longitudinal study was carried out to determine the clinical presentation and anatomical distribution of gross lesions in affected pigs in mbeya region, tanzania during the 2010 to 2014 outbreaks. data were collected during clinical and postmortem examination by field veterinarians and using a structured questionnaire. a total of 118 respondents (100%) showed awareness about african swin ... | 2017 | 27915438 |
| influence of age and dose of african swine fever virus infections on clinical outcome and blood parameters in pigs. | african swine fever (asf) is a fatal disease for domestic pigs, leading to serious economic losses in countries where asf is endemic. despite extensive research, efficient vaccines against asf are lacking. since peripheral blood cells are important mediators for vaccines, we study the impact of asf on blood parameters in pigs with different ages and infected with different doses of asf virus. four different groups were studied: (1) 12 weeks of age/low virus dose; (2) 12 weeks of age/high virus d ... | 2017 | 27875662 |
| simultaneous deletion of the 9gl and uk genes from the african swine fever virus georgia 2007 isolate offers increased safety and protection against homologous challenge. | african swine fever virus (asfv) is the etiological agent of a contagious and often lethal viral disease of domestic pigs that has significant economic consequences for the swine industry. the control of african swine fever (asf) has been hampered by the unavailability of vaccines. successful experimental vaccines have been derived from naturally occurring, cell culture-adapted, or genetically modified live attenuated asfv. recombinant viruses harboring engineered deletions of specific virulence ... | 2017 | 27795430 |
| spatio-temporal analysis of african swine fever in sardinia (2012-2014): trends in domestic pigs and wild boar. | african swine fever (asf) is a notifiable viral disease affecting domestic pigs and wild boars that has been endemic in sardinia since 1978. several risk factors complicate the control of asf in sardinia: generally poor level of biosecurity, traditional breeding practices, illegal behaviour in movements and feeding of pigs, and sporadic occurrence of long-term carriers. a previous study describes the disease in sardinia during 1978-2013. the aim of this study was to gain more in-depth knowledge ... | 2017 | 26392004 |
| statistical exploration of local transmission routes for african swine fever in pigs in the russian federation, 2007-2014. | african swine fever (asf) is a devastating viral disease of swine that is present in both pigs and wild boar in the western part of the russian federation and the eastern part of the european union. it represents a significant threat for the european pig production industry as neither treatment nor vaccine is available. this study analysed the spatial and spatio-temporal distributions of asf cases that were reported in domestic pigs and wild boar for assessing the likelihood of wild boar-to-dome ... | 2017 | 26192820 |
| neurophysiological monitoring during acute and progressive experimentally induced compression injury of the spinal cord in pigs. | to evaluate the degree of acute or progressive lateral compression needed to cause neurologic injury to the spinal cord assessed by electrophysiological monitoring. | 2017 | 25862652 |
| the 'heritability' of domestication and its functional partitioning in the pig. | we propose to estimate the proportion of variance explained by regression on genome-wide markers (or genomic heritability) when wild/domestic status is considered the phenotype of interest. this approach differs from the standard fst in that it can accommodate genetic similarity between individuals in a general form. we apply this strategy to complete genome data from 47 wild and domestic pigs from asia and europe. when we partitioned the total genomic variance into components associated to subs ... | 2017 | 27649617 |
| feasibility of electromagnetically guided transjugular intrahepatic portosystemic shunt procedure. | to develop an electromagnetic navigation technology for transjugular intrahepatic portosystemic shunt (tips) creation and translate it from phantom to an in-vivo large animal setting. | 2017 | 27686414 |
| osteoblast-like cells with different embryologic origins behave differently in increasing zoledronic acid concentrations: a pilot study in pigs. | we compared the intraindividual effects of increasing zoledronic acid (za) concentrations on osteoblast-like cells with different embryologic origins. | 2017 | 27720655 |
| septic porcine blood does not further activate coagulation during in vitro membrane oxygenation. | for patients with a severe acute respiratory distress syndrome (ards), extracorporeal membrane oxygenation (ecmo) represents a life-saving measure. frequently, patients with severe ards also show signs of severe sepsis. as blood contact with the membrane oxygenator surface leads to adverse effects due to insufficient biocompatibility partly caused by activation of platelets, coagulation factors and leucocytes, we hypothesized that these adverse effects would be amplified if septic blood in a pre ... | 2017 | 27806995 |
| creation of an animal model for long gap pure esophageal atresia. | long gap pure esophageal atresia (lgpea) is a congenital disorder in which the esophagus is in discontinuity, and the proximal and distal ends cannot be anastomosed in a primary fashion. no animal model for pure esophageal atresia exists. here we describe a survival animal model for lgpea, which will ultimately serve to test novel devices and techniques to restore continuity. | 2017 | 27838766 |
| glucocorticoid receptor number and affinity differ between peripheral blood mononuclear cells and granulocytes in domestic pigs. | the aim of the present study was to characterize the number and affinity of glucocorticoid receptors (gr) in peripheral blood mononuclear cells (pbmc) and granulocytes of domestic pigs because glucocorticoid signaling is considered important for animal health and welfare. to investigate gr binding characteristics in intact porcine immune cells, blood samples of 6 castrated male pigs were collected via indwelling vein catheters. porcine pbmc and granulocytes were isolated using two-layer density ... | 2017 | 28554109 |
| atrial myofibroblast activation and connective tissue formation in a porcine model of atrial fibrillation and reduced left ventricular function. | atrial fibrillation (af) is associated with fibrosis that slows electrical conduction and causes perpetuation of the arrhythmia. the molecular characterization of af pathophysiology may provide novel therapeutic options. this study was designed to elucidate profibrotic signaling and myofibroblast activation in a porcine model of atrial tachypacing-induced af and reduced left ventricular function. | 2017 | 28546006 |
| biosynthetic nanostructured cellulose patch for chest wall reconstruction: five-month follow-up in a porcine model. | ideal approaches and materials for reconstruction of large chest wall defects remain a topic of debate. we sought to explore the suitability of a reinforced nanostructured cellulose (nc) patch for chest wall reconstruction in an animal model. | 2017 | 27768401 |
| percutaneous externally assembled laparoscopic vs laparoendoscopic single-site nephrectomy in a porcine model: a prospective, randomized, blinded, study. | to compare the outcomes between laparoendoscopic single-site (less) and a novel percutaneous externally assembled laparoscopic (peal) nephrectomy in an in vivo porcine model. | 2017 | 27917649 |
| early and sustained vasopressin infusion augments the hemodynamic efficacy of restrictive fluid resuscitation and improves survival in a liver laceration model of hemorrhagic shock. | current management of hemorrhagic shock favors restrictive fluid resuscitation before control of the bleeding source. we investigated the additional effects of early and sustained vasopressin infusion in a swine model of hemorrhagic shock produced by liver laceration. | 2017 | 27906869 |
| open versus thoracoscopic rfa-assisted lung resection. | the purpose of this study was to evaluate radio frequency ablation (rfa)-assisted lung parenchymal transection through thoracotomy and thoracoscopy. twelve domestic pigs underwent rfa-assisted lingulectomy: six through thoracotomy (group a), and six with thoracoscopy (group b). there was no mortality, no bleeding, or air leak intra- or postoperatively in either of the groups, and no conversion to open thoracotomy in group b. group a had longer operating period and more pleural adhesions. a barot ... | 2017 | 27875060 |
| normal developmental and estrous cycle-dependent histological features of the female reproductive organs in microminipigs. | the microminipig has become an increasingly attractive animal model for various experimental practices because of its manageable size; however, studies of the histological features of the female reproductive organs in microminipigs are limited. the present study investigates the sexual development of the reproductive organs and the cyclical changes during the estrous cycle in female microminipigs. the ovaries, oviducts, uteri, and vaginal tissues from 33 animals aged 0 to 26 months were utilized ... | 2017 | 28580886 |
| mastering lymphatic microsurgery: a new training model in living tissue. | advanced microsurgical techniques have emerged as a promising approach for the treatment of lymphedema, but achieving international standards is limited by a scarcity of adequate training models. the purpose of this report is to describe our in vivo porcine training model for microsurgery. | 2017 | 28570444 |
| renal adiposity confounds quantitative assessment of markers of renal diffusion with mri: a proposed correction method. | recent studies have indicated that excessive fat may confound assessment of diffusion in organs with high fat content, such as the liver and breast. however, the extent of this effect in the kidney, which is not considered a major fat deposition site, remains unclear. this study tested the hypothesis that renal fat may impact diffusion-weighted imaging (dwi) parameters, and proposes a 3-compartment model (tcm) to circumvent this effect. | 2017 | 28562413 |
| role of stro-1 sorting of porcine dental germ stem cells in dental stem cell-mediated bone tissue engineering. | stem cells of dental origin emerged as a new source for the regeneration of tissues with advantages mainly including non-invasive collection procedures and lack of ethical contraversies with their harvest or use. in this study, porcine tgscs (ptgscs) were isolated from mandibular third molar tooth germs of 6-month-old domestic pigs. this is the first study that reports the isolation and characterization of tgscs from porcine third molars and their differentiation depending on stro-1 expression. ... | 2017 | 28562085 |
| preclinical pilot study monitoring topical drug penetration and dermal bioavailability of a peptidase inhibitor from different galenic formulations into pig dermis, using cutaneous microdialysis. | cutaneous microdialysis (cm) is an ex vivo technique that allows study of tissue chemistry, including bioavailability of actual tissue concentration of unbound drug in the interstitial fluid of the body. | 2017 | 28556026 |
| the relationship between surface area and volume of common blow fly (diptera: calliphoridae) oviposition sites and carrion body mass. | female blow flies (diptera: calliphoridae) demonstrate site preferences when ovipositing on carrion. preference for oviposition sites is believed to reflect the suitability of the site such that the most preferred locations provide offspring with the greatest chance of survival. a number of factors might influence female choice, including area available for oviposition. for species known to aggregate during oviposition, the surface area of oviposition sites may be a key factor in the decision-ma ... | 2017 | 28535281 |
| plasma concentrations of transdermal fentanyl and buprenorphine in pigs (sus scrofa domesticus). | to determine the absorption characteristics of fentanyl and buprenorphine administered transdermally in swine. | 2017 | 28526486 |
| detection and evaluation of renal injury in burst wave lithotripsy using ultrasound and magnetic resonance imaging. | burst wave lithotripsy (bwl) is a transcutaneous technique with potential to safely and effectively fragment renal stones. preclinical investigations of bwl require the assessment of potential renal injury. this study evaluates the capabilities of real-time ultrasound and mri to detect and evaluate bwl injury that was induced in porcine kidneys. | 2017 | 28521550 |
| differential expression of genes related to glucose metabolism in domesticated pigs and wild boar. | glucose metabolism is a basic biological process that shows substantial variation within and between species. using pig as a model organism, we investigated differences in glucose metabolic genes in seven tissues from domesticated pigs (rongchang pig and tibetan pig, meanwhile, the tibetan pig just as a special case of the domesticated pig under plateau condition) and wild boar. we found large differences in the expression of genes involved in multiple aspects of glucose metabolism, including ge ... | 2017 | 28508704 |
| a pathway-centered analysis of pig domestication and breeding in eurasia. | ascertaining the molecular and physiological basis of domestication and breeding is an active area of research. due to the current wide distribution of its wild ancestor, the wild boar, the pig (sus scrofa) is an excellent model to study these processes, which occurred independently in east asia and europe ca. 9000 yr ago. analyzing genome variability patterns in terms of metabolic pathways is attractive since it considers the impact of interrelated functions of genes, in contrast to genome-wide ... | 2017 | 28500056 |
| metformin prevents ischaemic ventricular fibrillation in metabolically normal pigs. | metformin is the drug most often used to treat type 2 diabetes. evidence suggests that metformin may reduce mortality of individuals with type 2 diabetes, but the mechanism of such an effect is unknown and outcomes of metformin treatment in people without diabetes have not been determined. if metformin favourably affected mortality of non-diabetic individuals, it might have even broader therapeutic utility. we evaluated the effect of metformin on myocardial energetics and ischaemic ventricular f ... | 2017 | 28497164 |
| a porcineex vivolung perfusion model with maximal argon exposure to attenuate ischemia-reperfusion injury. | argon (ar) is a noble gas with known organoprotective effects in rodents andin vitromodels. in a previous study we failed to find a postconditioning effect of ar duringex vivolung perfusion (evlp) on warm-ischemic injury in a porcine model. in this study, we further investigated a prolonged exposure to ar to decrease cold ischemia-reperfusion injury after lung transplantation in a porcine model with evlp assessment. domestic pigs (n= 6/group) were pre-conditioned for 6 hours with 21% o2and 79% n ... | 2017 | 28480029 |
| insular east asia pig dispersal and vicariance inferred from asian wild boar genetic evidence. | the formosan wild boar () is an endemic subspecies in taiwan. understanding the origins and spread of the formosan wild boar could help clarify east asian wild boar dispersion. although in situ domestication of the wild boar occurred at a number of domestication centers across east asia, corroborating archaeological and genetic evidence of pig domestication on taiwan is lacking, leading to domestication being described as cryptic. this characterization applies to the lanyu pig-a domestic pig bre ... | 2017 | 28464072 |
| titanium implant functionalization with phosphate-containing polymers may favour in vivo osseointegration. | osseointegration of titanium implants is predictable, but can be improved via surface functionalization. | 2017 | 28453878 |
| magnetic resonance imaging as a noninvasive method for longitudinal monitoring of infusion site reactions following administration of a novel apomorphine formulation. | infusion site reactions are common following subcutaneous infusion of drugs. such reactions can lead to discontinuation of the treatment. therefore, assessment of such reactions is essential during preclinical safety studies, and magnetic resonance imaging (mri) can assist in evaluation. here, in vivo and ex vivo mri evaluations were used in addition to classical histopathology to assess the infusion site reaction to nd0701, a novel formulation of apomorphine base developed for the treatment of ... | 2017 | 28443458 |
| heart-rate variability depression in porcine peritonitis-induced sepsis without organ failure. | depression of heart-rate variability (hrv) in conditions of systemic inflammation has been shown in both patients and experimental animal models and hrv has been suggested as an early indicator of sepsis. the sensitivity of hrv-derived parameters to the severity of sepsis, however, remains unclear. in this study we modified the clinically relevant porcine model of peritonitis-induced sepsis in order to avoid the development of organ failure and to test the sensitivity of hrv to such non-severe c ... | 2017 | 28440737 |
| classical swine fever-an updated review. | classical swine fever (csf) remains one of the most important transboundary viral diseases of swine worldwide. the causative agent is csf virus, a small, enveloped rna virus of the genus pestivirus. based on partial sequences, three genotypes can be distinguished that do not, however, directly correlate with virulence. depending on both virus and host factors, a wide range of clinical syndromes can be observed and thus, laboratory confirmation is mandatory. to this means, both direct and indirec ... | 2017 | 28430168 |
| proteomic analysis of the endometrium during early pregnancy in the domestic pig. | reproductive processes in domestic pigs have been studied extensively. pigs are one of the main sources of meat for human consumption and are an established model for investigations into mammalian, including human, reproductive physiology. studies of the uterus during early pregnancy will lead to a better understanding of mechanisms governing pregnancy. proteomics provides the possibility to explore endometrial functions in an unbiased way. the aim of the study was to compare endometrium harvest ... | 2017 | 28416050 |
| a simple method of placing a coronary sinus catheter through the femoral vein in miniature swine. | the aim of the present study was to evaluate the feasibility of placing a coronary sinus (cs) catheter through the femoral veins of miniature swine. a total of 16 male domestic pigs (3-4 months old, 25±2 kg) were used. firstly, the anatomic structure of the cs ostium of swine heart was observed at different angles under x-ray. the guide wire and cobara catheter were subsequently advanced into the right atrium through the femoral vein. subsequently, the guide wire was retracted behind the fix cur ... | 2017 | 28413516 |
| effects of racecadotril on weight loss and diarrhea due to human rotavirus in neonatal gnotobiotic pigs (sus scrofa domesticus). | diarrheal disease is the second leading cause of death in children younger than 5 y, and the most common cause of acute watery diarrhea in young children worldwide is rotaviral infection. medicines to specifically reduce diarrhea would be a desirable adjunctive treatment to supportive fluid therapy to decrease the mortality rate of diarrheal diseases. in this study, we evaluated the efficacy of an antisecretory drug, racecadotril, in treating human rotavirus (hrv)-induced diarrhea in a neonatal ... | 2017 | 28381316 |
| novel effects of identified snps within the porcine pregnancy-associated glycoprotein gene family (ppags) on the major reproductive traits in hirschmann hybrid-line sows. | this is the first study describing identification of snps within the multiple and polymorphic pregnancy-associated glycoprotein gene family (pags) in the genome of the domestic pig (ppags). we identified ppag-like (ppag-l) genotypes in primiparous and multiparous farmed hybrid-line jsr hirschmann (hrn) sows (n=159), in which various novel associations with their phenotypes for the major reproductive traits have been discovered. genomic dna templates were isolated from the blood and different ppa ... | 2017 | 28371694 |
| featured article: pyruvate preserves antiglycation defenses in porcine brain after cardiac arrest. | cardiac arrest (ca) and cardiocerebral resuscitation (ccr)-induced ischemia-reperfusion imposes oxidative and carbonyl stress that injures the brain. the ischemic shift to anaerobic glycolysis, combined with oxyradical inactivation of glyceraldehyde 3-phosphate dehydrogenase (gapdh), provokes excessive formation of the powerful glycating agent, methylglyoxal. the glyoxalase (glo) system, comprising the enzymes glyoxalase 1 (glo1) and glo2, utilizes reduced glutathione (gsh) supplied by glutathio ... | 2017 | 28361585 |
| defective postsecretory maturation of muc5b mucin in cystic fibrosis airways. | in cystic fibrosis (cf), airway mucus becomes thick and viscous, and its clearance from the airways is impaired. the gel-forming mucins undergo an ordered "unpacking/maturation" process after granular release that requires an optimum postsecretory environment, including hydration and ph. we hypothesized that this unpacking process is compromised in the cf lung due to abnormal transepithelial fluid transport that reduces airway surface hydration and alters ionic composition. using human tracheobr ... | 2017 | 28352653 |
| histological and anatomical structure of the nasal cavity of bama minipigs. | the nasal mucosa is equipped with abundant lymphatic tissues, serving as the first line of defense against invasion by microorganisms. in this study, we characterized the features of the nasal mucosa of bama minipigs (sus scrofa domestica) via histological analysis. | 2017 | 28339502 |
| the early-life environment of a pig shapes the phenotypes of its social partners in adulthood. | social interactions among individuals are abundant, both in natural and domestic populations, and may affect phenotypes of individuals. recent research has demonstrated that the social effect of an individual on the phenotype of its social partners may have a genetic component, known as an indirect genetic effect (ige). little is known, however, of nongenetic factors underlying such social effects. early-life environments often have large effects on phenotypes of the individuals themselves later ... | 2017 | 28327581 |
| intra-luminal gene therapy in the porcine artery using a recombinant adeno-associated virus 9. | the ability to improve or restore blood flow and promote healing in ischemic tissue has many potential clinical applications. augmentation by direct delivery of growth factors may further enhance results, but requires a method for sustained delivery. in this study, we have tested the ability of adeno-associated virus 9 (aav9) delivered within the lumen of a porcine artery to transfect the vessel and produce a desired product. the marker chosen was green fluorescent protein (gfp) (ke et al., 2011 ... | 2017 | 28322993 |
| cardiovascular disease and resuscitated septic shock lead to the downregulation of the h2s-producing enzyme cystathionine-γ-lyase in the porcine coronary artery. | downregulation of the hydrogen sulfide (h2s)-producing enzymes cystathionine-γ-lyase (cse), cystathionine-β-synthase (cbs), and/or 3-mercaptopyruvate sulfurtransferase (3-mst) is associated with chronic cardiovascular pathologies. nevertheless, equivocal data are available on both the expression and function of these enzymes in coronary arteries (ca). we recently reported that atherosclerotic pigs subjected to sepsis developed impaired cardiac function, which coincided with decreased myocardial ... | 2017 | 28321823 |