Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| inhibition of alpha/beta interferon signaling by the ns4b protein of flaviviruses. | flaviviruses are insect-borne, positive-strand rna viruses that have been disseminated worldwide. their genome is translated into a polyprotein, which is subsequently cleaved by a combination of viral and host proteases to produce three structural proteins and seven nonstructural proteins. the nonstructural protein ns4b of dengue 2 virus partially blocks activation of stat1 and interferon-stimulated response element (isre) promoters in cells stimulated with interferon (ifn). we have found that t ... | 2005 | 15956546 |
| comparison of contact and spatial repellency of catnip oil and n,n-diethyl-3-methylbenzamide (deet) against mosquitoes. | nepetalactone, the primary component of catnip oil, was compared with the repellent n,n-diethyl-3-methylbenzamide (deet) for its ability to affect the host-seeking ability of aedes aegypti (l.). a triple cage olfactometer was used to bioassay each substance and to assess its attraction inhibition (spatial repellent) attributes when combined with the following attractants: carbon dioxide, acetone, a blend of l-lactic acid and acetone, and human odors. repellent tests were conducted with each subs ... | 2005 | 15962779 |
| synthesis of additional endotoxins in bacillus thuringiensis subsp. morrisoni pg-14 and bacillus thuringiensis subsp. jegathesan significantly improves their mosquitocidal efficacy. | a fundamental principal of resistance management is that the more complex and potent a toxin mixture, the slower resistance will develop to the mixture in an insect population. thus, to develop more complex and potent mosquitocidal bacteria, we genetically engineered bacillus thuringiensis subsp. morrisoni pg-14 and bacillus thuringiensis subsp. jegathesan, to synthesize, respectively, the binary (bin) toxin of bacillus sphaericus or a combination of bin and the cytla protein of bacillus thuring ... | 2005 | 15962784 |
| use of novel compounds for pest control: insecticidal and acaricidal activity of essential oil components from heartwood of alaska yellow cedar. | laboratory bioassays were conducted to determine the activity of 15 natural products isolated from essential oil components extracted from the heartwood of alaska yellow cedar, chamaecyparis nootkatensis (d. don) spach., against ixodes scapularis say nymphs, xenopsylla cheopis (rothchild), and aedes aegypti (l.) adults. four of the compounds from the essential oil have been identified as monoterpenes, five as eremophilane sesquiterpenes, five as eremophilane sesquiterpene derivatives from valenc ... | 2005 | 15962787 |
| [characteristics of new cell cultures derived from embryonic tissues of aedes aegypti (diptera: culicidae)]. | cell cultures from insects are a useful methodology in technological and biomedical studies. | 2005 | 15962903 |
| cry11aa toxin from bacillus thuringiensis binds its receptor in aedes aegypti mosquito larvae through loop alpha-8 of domain ii. | bacillus thuringiensis subs israelensis produces cry toxins active against mosquitoes. receptor binding is a key determinant for specificity of cry toxins composed of three domains. we found that exposed loop alpha-8 of cry11aa toxin, located in domain ii, is an important epitope involved in receptor interaction. synthetic peptides corresponding to exposed regions in domain ii (loop alpha-8, beta-4 and loop 3) competed binding of cry11aa to membrane vesicles from aedes aegypti midgut microvilli. ... | 2005 | 15963509 |
| dengue in grenada. | dengue fever is endemic in the country of grenada and is grossly underreported as a source of morbidity. the goal of this study was to assess the status of dengue fever in a representative community in grenada. | 2005 | 15969973 |
| knowledge and attitudes in puerto rico concerning dengue prevention. | dengue has been endemic in puerto rico for three decades. multiple educational and community-based efforts have been developed to inform the population about dengue prevention. we undertook this study to understand the community members' knowledge, attitudes, and practices related to dengue prevention and to elicit their ideas for future prevention campaigns. | 2005 | 15969976 |
| antennal expressed genes of the yellow fever mosquito (aedes aegypti l.); characterization of odorant-binding protein 10 and takeout. | a small cdna library was constructed from antennae of 100 adult male aedes aegypti yellow fever mosquitoes. sequencing of 80 clones identified 49 unique gene products, including a member of the odorant binding protein family (aaeg-obp10), a homologue of takeout (aaeg-to), and transposable elements of the line, sine and mite classes. aaeg-obp10 encodes a 140 amino acid protein including a predicted 25 amino acid signal peptide. aaeg-obp10 expression was adult male enriched, increased with adult a ... | 2005 | 15978998 |
| time-specific ecological niche modeling predicts spatial dynamics of vector insects and human dengue cases. | numerous human diseases-malaria, dengue, yellow fever and leishmaniasis, to name a few-are transmitted by insect vectors with brief life cycles and biting activity that varies in both space and time. although the general geographic distributions of these epidemiologically important species are known, the spatiotemporal variation in their emergence and activity remains poorly understood. we used ecological niche modeling via a genetic algorithm to produce time-specific predictive models of monthl ... | 2005 | 15979656 |
| cloning and characterization of cdnas encoding putative ctcfs in the mosquitoes, aedes aegypti and anopheles gambiae. | one of the many ascribed functions of ccctc-binding factor (ctcf) in vertebrates is insulation of genes via enhancer-blocking. insulation allows genes to be shielded from "cross-talk" with neighboring regulatory elements. as such, endogenous insulator sequences would be valuable elements to enable stable transgene expression. recently, ctcf joined su(hw), zw5, beaf32 and gaga factor as a protein associated with insulator activity in the fruitfly, drosophila melanogaster. to date, no known insula ... | 2005 | 15985163 |
| detailed assessment of microhabitat suitability for aedes aegypti (diptera: culicidae) in buenos aires, argentina. | little information is available on the ecology of aedes aegypti linnaeus at the southern extreme of its distribution (buenos aires, argentina), particularly on microhabitat suitability. the aim of our study was to identify at a detailed scale, microhabitat factors that correlate with the presence of preimaginal stages of the mosquito. in march 2001, we performed a spatial census of all containers located in a 1 ha patch within a cemetery in buenos aires city. on a reference map (1:700) we plotte ... | 2005 | 15993832 |
| castanospermine, a potent inhibitor of dengue virus infection in vitro and in vivo. | previous studies have suggested that alpha-glucosidase inhibitors such as castanospermine and deoxynojirimycin inhibit dengue virus type 1 infection by disrupting the folding of the structural proteins prm and e, a step crucial to viral secretion. we extend these studies by evaluating the inhibitory activity of castanospermine against a panel of clinically important flaviviruses including all four serotypes of dengue virus, yellow fever virus, and west nile virus. using in vitro assays we demons ... | 2005 | 15994763 |
| factors influencing the population structure of aedes aegypti from the main cities in cambodia. | a population genetic analysis was conducted on 47 aedes aegypti collections from cambodia. genetic differentiation at seven polymorphic isoenzyme loci was analysed by starch gel electrophoresis. low (f(st)=0.024) but significant (p<10(-6)) differentiation was found when all samples were considered. whatever the grouping of samples tested, differentiation remained significant but low. the role of human activities (ie insecticide treatments or water storage practices) and environmental factors (ie ... | 2005 | 15999142 |
| review of microsporidia-mosquito relationships: from the simple to the complex. | microsporidia in mosquitoes can be divided into two categories based on their life cycles and host-parasite relationships. some species of microsporidia exhibit simple life cycles with one spore type responsible for oral (horizontal) transmission. they affect only one generation of the mosquito and are not usually host or tissue specific. brachiola algerae and vavraia culicis are examples of species isolated from mosquitoes with relatively straightforward life cycles (one spore type) and simple ... | 2005 | 16004363 |
| characterization of deltamethrin resistance in field populations of aedes aegypti in thailand. | five field collections of adult aedes aegypti mosquitoes from different areas in bangkok and pathum thani provinces were subjected to susceptibility tests against deltamethrin. low levels of resistance were detected among all populations tested (rr50 = 8-17.2) compared to the susceptible strain, bora (french polynesia). among the five populations tested, the bkh (bang khen, bangkok) and psc (phasicharoen, bangkok) populations showed a higher level of deltamethrin resistance than the other three ... | 2005 | 16007969 |
| morphological and molecular characterization of a cypovirus (reoviridae) from the mosquito uranotaenia sapphirina (diptera: culicidae). | a novel cypovirus has been isolated from the mosquito uranotaenia sapphirina (uscpv) and shown to cause a chronic infection confined to the cytoplasm of epithelial cells of the gastric ceca and posterior stomach. the production of large numbers of virions and inclusion bodies and their arrangement into paracrystalline arrays gives the gut of infected insects a distinctive blue iridescence. the virions, which were examined by electron microscopy, are icosahedral (55 to 65 nm in diameter) with a c ... | 2005 | 16014906 |
| residual effect of two bacillus thuringiensis var. israelensis products assayed against aedes aegypti (diptera: culicidae) in laboratory and outdoors at rio de janeiro, brazil. | resistance of the dengue vector to temephos stimulated its substitution for bacillus thuringiensis var. israelensis (bti) since 2001 in brazil. the persistence of the two bti formulations employed at that time by the health ministry, vectobac g and aquabac g, was assayed under laboratory and outdoor conditions. both formulations were tested at 0.2 g/10 liters of water, the same concentration applied in the field for vector control. the tests were done against ae. aegypti third instar larvae (roc ... | 2005 | 16021284 |
| fructose variation in the dengue vector, aedes aegypti, during high and low transmission seasons in the mae sot region of thailand. | to understand the potential for facultative sugar feeding by aedes aegypti, an important vector of dengue viruses, we evaluated seasonal and villagewide variation in sugar feeding during high- and low-dengue transmission seasons (rainy and dry seasons, respectively) in the mae sot region of thailand. these seasons in thailand are represented by different periods of flowering plant phenology. although overall sugar feeding among female and male mosquitoes was low (ranging from 0.60 to 7.53 microg ... | 2005 | 16033119 |
| testing vitamin b as a home remedy against mosquitoes. | vitamin b is often recommended in the popular media as a systemic repellent against mosquitoes. this information is especially prevalent on the web. the results of a small number of published studies suggested that vitamin b complex supplements are not effective as repellents, but these studies were limited by the use of very few human subjects and only 1 species of mosquito, aedes aegypti. we extended this work with the use of larger samples of human subjects and with anopheles stephensi as the ... | 2005 | 16033124 |
| laboratory evaluation of mosquito traps baited with a synthetic human odor blend to capture aedes aegypti. | a synthetic blend of chemicals comprising volatiles released by the human body has been shown to be an effective attractant for female aedes aegypti in olfactometer bioassays with laboratory-reared mosquitoes. we report the laboratory evaluation of ae. aegypti response to a synthetic blend tested with 4 types of mosquito traps (cdc model 512, cdc model 1012, cfg, and fay-prince traps). aedes aegypti females were attracted significantly by the blend. the higher release rate of attractant (320.2 + ... | 2005 | 16033129 |
| gene structure and expression profile of manduca sexta prophenoloxidase-activating proteinase-3 (pap-3), an immune protein containing two clip domains. | prophenoloxidase-activating proteinase-3 (pap-3) is a component of the defence system in manduca sexta. we have isolated genomic clones and elucidated the organization of this gene. the 3' end of exon 2, the entire exon 3 and the 5' end of exon 4 encode the two amino-terminal clip domains. southern blot analysis suggested a single copy of the pap-3 gene in the genome. we identified several putative immune-responsive elements in the upstream region. the pap-3 gene is not highly expressed in the f ... | 2005 | 16033436 |
| michelob_x is the missing inhibitor of apoptosis protein antagonist in mosquito genomes. | apoptosis is implicated in the life cycle of the malaria parasite in mosquitoes. the genome project for the primary malaria vector anopheles gambiae showed a significant expansion of the inhibitor of apoptosis protein (iap) and caspase gene families in comparison with drosophila. however, because of extensive sequence divergence, no orthologue was identified for the reaper/grim-like iap antagonist genes that have a pivotal role in cell death regulation in drosophila. using a customized searching ... | 2005 | 16041319 |
| comparative repellency of 38 essential oils against mosquito bites. | the mosquito repellent activity of 38 essential oils from plants at three concentrations was screened against the mosquito aedes aegypti under laboratory conditions using human subjects. on a volunteer's forearm, 0.1 ml of oil was applied per 30 cm2 of exposed skin. when the tested oils were applied at a 10% or 50% concentration, none of them prevented mosquito bites for as long as 2 h, but the undiluted oils of cymbopogon nardus (citronella), pogostemon cablin (patchuli), syzygium aromaticum (c ... | 2005 | 16041723 |
| carbon dioxide instantly sensitizes female yellow fever mosquitoes to human skin odours. | female mosquitoes are noted for their ability to use odours to locate a host for a blood meal. two sensory organs contribute to their sense of smell: the maxillary palps, which measure the level of co2, and the antennae, which detect other host-released odours. to establish the relative importance and interactions of co2 and other body emissions in freely flying mosquitoes, we presented female yellow fever mosquitoes aedes aegypti l. with broad plumes of human skin odour and co2 at natural conce ... | 2005 | 16043601 |
| impact of vector control on a dengue fever outbreak in trinidad, west indies, in 1998. | in 1998, trinidad experienced its first major outbreak of dengue haemorrhagic fever. data from the trinidad public health laboratory, the national surveillance unit and insect vector control division, ministry of health, trinidad and tobago were analysed to determine the impact of vector control measures on the dengue outbreak. geographical information systems (gis)/global positioning systems (gps) were used to map cases and to distinguish epidemiological clusters. the aedes aegypti population d ... | 2005 | 16045461 |
| effectiveness of dengue control practices in household water containers in northeast thailand. | to investigate the influence of larval control methods (using temephos, keeping fish and covering containers with lids), water use and weekly cleaning of containers on the presence of aedes aegypti larvae in water-storage containers in rural and urban households in khon kaen province. | 2005 | 16045462 |
| insecticidal, repellent and oviposition-deterrent activity of selected essential oils against anopheles stephensi, aedes aegypti and culex quinquefasciatus. | essential oils extracted from 10 medicinal plants were evaluated for larvicidal, adulticidal, ovicidal, oviposition-deterrent and repellent activities towards three mosquito species; anopheles stephensi, aedes aegypti and culex quinquefasciatus. the essential oils of juniperus macropoda and pimpinella anisum were highly effective as both larvicidal and ovicidal. the essential oil of p. anisum showed toxicity against 4th instar larvae of a. stephensi and a. aegypti with equivalent ld95 values of ... | 2005 | 16051081 |
| building a better ovitrap for detecting aedes aegypti oviposition. | 2005 | 16055072 | |
| cloning and characterization of two novel genes, cry24b and s1orf2, from a mosquitocidal strain of bacillus thuringiensis serovar sotto. | two new crystal protein genes, cry24b and s1orf2, were cloned from a mosquitocidal bacillus thuringiensis serovar sotto strain. the cry24b and s1orf2 genes encoded a 76-kda and 62-kda protein, respectively. the cry24b protein retained five conserved regions commonly found in the existing cry proteins. the amino acid sequence of the s1orf2 had a high homology to that of the orf2 protein of b. thuringiensis serovar jegathesan. southern hybridization experiments with a cry24b gene-specific probe re ... | 2005 | 16059769 |
| growth, sporulation, delta-endotoxins synthesis, and toxicity during culture of bacillus thuringiensis h14. | growth, sporulation, synthesis of delta-endotoxins, and toxicity against the larvae of aedes aegypti and culex pipiens were studied during fermentation of bacillus thuringiensis h14 in a 20-l fermentor. measurements of optical density and dielectric permittivity for biomass determination suggest a highly promising technique for on-line evaluation of sporulation. the synthesis of 65-, 25- and 130-kda proteins started at 16, 18, and 23 h, respectively. these proteins were enriched in different way ... | 2005 | 16059772 |
| inhibitor kappab-like proteins from a polydnavirus inhibit nf-kappab activation and suppress the insect immune response. | complex signaling pathways regulate the innate immune system of insects, with nf-kappab transcription factors playing a central role in the activation of antimicrobial peptides and other immune genes. although numerous studies have characterized the immune responses of insects to pathogens, comparatively little is known about the counter-strategies pathogens have evolved to circumvent host defenses. among the most potent immunosuppressive pathogens of insects are polydnaviruses that are symbioti ... | 2005 | 16061795 |
| phytochemical studies on the seed extract of piper nigrum linn. | the petroleum ether extract of dried ground seeds of piper nigrum linn. and some column fractions of this extract were subjected to gc and gc-ms analysis, resulting in the identification of fourteen compounds (1-14) by using nist mass spectral search program 1998 and the kovat's retention indices. ten of the compounds (1, 2, 4-12) are reported for the first time from this plant. all the fractions showed insecticidal activity against the fourth instar larvae of aedes aegypti and against the fourt ... | 2005 | 16076642 |
| immune signaling pathways regulating bacterial and malaria parasite infection of the mosquito anopheles gambiae. | we show that, in the malaria vector anopheles gambiae, expression of cecropin 1 is regulated by rel2, an nf-kappab-like transcription factor orthologous to drosophila relish. through alternative splicing, rel2 produces a full-length (rel2-f) and a shorter (rel2-s) protein isoform lacking the inhibitory ankyrin repeats and death domain. rna interference experiments show that, in contrast to drosophila relish, which responds solely to gram-negative bacteria, the anopheles rel2-f and rel2-s isoform ... | 2005 | 16076953 |
| [residual effect of commercial applications of larvicides temefos and bacillus thuringiensis israelensis on aedes aegypti larvae in recipients with water renewal]. | the residual effect of temefos (commercial applications a, b and c) and bacillus thuringiensis israelensis (d and e) on aedes aegypti larvae was evaluated, in recipients with water renewal. forty-four beakers of 1,000ml each (8 for each application and 4 controls) were used. in each beaker, 25 larvae were introduced daily. after 24 hours, the dead larvae were counted, the beakers emptied to 200ml and refilled to original volume, with addition of new larvae. the duration of the maximum residual e ... | 2005 | 16082478 |
| identification and analysis of ribonuclease p and mrp rna in a broad range of eukaryotes. | rnases p and mrp are ribonucleoprotein complexes involved in trna and rrna processing, respectively. the rna subunits of these two enzymes are structurally related to each other and play an essential role in the enzymatic reaction. both of the rnas have a highly conserved helical region, p4, which is important in the catalytic reaction. we have used a bioinformatics approach based on conserved elements to computationally analyze available genomic sequences of eukaryotic organisms and have identi ... | 2005 | 16087735 |
| an anti-chitinase malaria transmission-blocking single-chain antibody as an effector molecule for creating a plasmodium falciparum-refractory mosquito. | indirect evidence has suggested the existence of a second chitinase gene, pgcht2, in the avian malaria parasite plasmodium gallinaceum. we have now identified pgcht2 as the orthologue of the p. falciparum chitinase gene pfcht1, a malaria transmission-blocking target. computational phylogenetic evidence and biochemical and cell biological functional data support the hypothesis that an avian-related plasmodium species was the ancestor of both p. falciparum and p. reichenowi, and this single lineag ... | 2005 | 16088838 |
| expression of the cry11a gene of bacillus thuringiensis ssp. israelensis in saccharomyces cerevisiae. | the complete cry11a region gene of bacillus thuringiensis ssp. israelensis was fused in frame to the 3' end of the gst gene under the control of the saccharomyces cerevisiae hxk1 promoter. the fusion protein gst-cry11a was expressed in s. cerevisiae strain amw13c+. the fusion gene gst-cry11a was expressed when yeast cells were grown on galactose and a nonfermentable medium containing ethanol as carbon and energy source. when the cells were grown in glucose, mannose, fructose, or glycerol as carb ... | 2005 | 16091775 |
| development and characterization of a double subgenomic chikungunya virus infectious clone to express heterologous genes in aedes aegypti mosquitoes. | three full-length infectious cdna clones based on the alphavirus chikungunya (chikv) were developed and characterized in vitro and in vivo. the full-length clone retained the viral phenotypes of chikv in both cell culture and in mosquitoes and should be a valuable tool for the study of virus interactions in an epidemiologically significant natural vector, aedes aegypti. two additional infectious clones were constructed that express green fluorescent protein (egfp) in the midgut, salivary glands, ... | 2005 | 16102421 |
| mutant mos1 mariner transposons are hyperactive in aedes aegypti. | the development of genetic strategies to control the spread of mosquito-borne diseases through the use of class ii transposons has been hampered by suboptimal rates of transformation and the absence of post-integration mobility for all transposons evaluated to date. two mos1 mariner transposase mutants were produced by the site-directed mutagenesis of amino acids, e137 and e264, to k and r, respectively. the effects of these mutations on the transpositional activities of mos1-derived transposon ... | 2005 | 16102425 |
| dengue virus nonstructural protein ns5 induces interleukin-8 transcription and secretion. | elevated circulating levels of chemokines have been reported in patients with dengue fever and are proposed to contribute to the pathogenesis of dengue disease. to establish in vitro models for chemokine induction by dengue 2 virus (den2v), we studied a variety of human cell lines and primary cells. den2v infection of hepg2 and primary dendritic cells induced the production of interleukin-8 (il-8), rantes, mip-1alpha, and mip-1beta, whereas only il-8 and rantes were induced following dengue viru ... | 2005 | 16103156 |
| the secreted form of dengue virus nonstructural protein ns1 is endocytosed by hepatocytes and accumulates in late endosomes: implications for viral infectivity. | the flavivirus nonstructural protein ns1 is expressed as three discrete species in infected mammalian cells: an intracellular, membrane-associated form essential for viral replication, a cell surface-associated form that may be involved in signal transduction, and a secreted form (sns1), the biological properties of which remain elusive. to determine the distribution of the dengue virus (den) sns1 protein in vivo, we have analyzed by immunohistological means the tissue tropism of purified den sn ... | 2005 | 16103191 |
| application of an alternative aedes species (diptera: culicidae) surveillance method in botucatu city, sao paulo, brazil. | one of the main problems with dengue is the control of aedes aegypti, its major vector. in brazil, the current control program for ae. aegypti and aedes albopictus populations includes larval density surveys. an interesting alternative is the use of a distinct index, the premise condition index (pci). this tool relates conditions of property, such as houses and yards, and the degree of shade with the occurrence of aedes sp. oviposition, and is calculated as scores from 3 to 9. the lowest score i ... | 2005 | 16103596 |
| field evaluation of spatial repellency of metofluthrin impregnated plastic strips against mosquitoes in hai phong city, vietnam. | spatial repellency of metofluthrin-impregnated polyethylene plastic strips against mosquitoes, aedes aegypti and culex quinquefasciatus, were studied in a residential area in hai phong city, vietnam. thirty houses were selected as trial sites; half of these were assigned as untreated control and the other half were assigned for treatment. primarily, irrespective of the room size, one room was treated with one strip. the dominant species in the trial sites were culex quinquefasciatus and aedes ae ... | 2005 | 16103602 |
| potential role of sylvatic and domestic african mosquito species in dengue emergence. | dengue virus 2 (denv-2) strains that circulate in sylvatic habitats of senegal and other parts of west africa are believed to represent ancestral forms that evolved into endemic/epidemic strains that now circulate widely in urban areas of the tropics. previous studies suggested that the evolution of the endemic/epidemic strains was mediated by adaptation to the peridomestic mosquito vectors aedes aegypti and ae. albopictus. we conducted experimental infections using sylvatic and peridomestic sen ... | 2005 | 16103619 |
| enhanced t-cell response to mosquito extracts by nk cells in hypersensitivity to mosquito bites associated with ebv infection and nk cell lymphocytosis. | hypersensitivity to mosquito bites is characterized by severe systemic as well as local symptoms, and associated with chronic active ebv infection and nk cell lymphocytosis. in this hen disease, we investigated the response of pbmc to msg extracts. pbmc were taken from three defined cases of hen disease, three borderline cases, five individuals with simple exaggerated reactions to mosquito bites without systemic symptoms (simple responders), and eight healthy donors. pbmc, or purified cd4+, cd8+ ... | 2005 | 16108834 |
| mosquito natriuretic peptide identified as a calcitonin-like diuretic hormone in anopheles gambiae (giles). | mosquito natriuretic peptide (mnp), an uncharacterised peptide from the yellow fever mosquito, aedes aegypti, acts via cyclic amp to stimulate secretion of na+-rich urine by opening a na+ conductance in the basolateral membrane of malpighian tubule principal cells. corticotropin releasing factor (crf)-related peptides and calcitonin (ct)-like diuretic peptides use cyclic amp as a second messenger and were therefore considered likely candidates for mnp. blast searches of the genome of the malaria ... | 2005 | 16109890 |
| anopheles gambiae srpn2 facilitates midgut invasion by the malaria parasite plasmodium berghei. | we report on a phylogenetic and functional analysis of genes encoding three mosquito serpins (srpn1, srpn2 and srpn3), which resemble known inhibitors of prophenoloxidase-activating enzymes in other insects. following rna interference induction by double-stranded rna injection, knockdown of srpn2 in adult anopheles gambiae produced a notable phenotype: the appearance of melanotic pseudotumours, which increased in size and number with time, indicating spontaneous melanization and association with ... | 2005 | 16113656 |
| tick saliva is a potent inhibitor of endothelial cell proliferation and angiogenesis. | we report for the first time that saliva of the hard tick and lyme disease vector, ixodes scapularis, is a potent inhibitor of angiogenesis. saliva (< or = 1:500 dilutions) or salivary gland (0.1-0.5 pairs/assay) dose-dependently inhibits microvascular endothelial cell (mvec) proliferation. inhibition was also detected with the saliva of the cattle tick boophilus microplus but not with the salivary gland of anopheles gambiae, an. stephensi, lutzomyia longipalpis, phlebotomus papatasi, aedes aegy ... | 2005 | 16113800 |
| identification of the best ovitrap installation sites for gravid aedes (stegomyia) aegypti in residences in mirassol, state of são paulo, brazil. | this study aimed at identifying the best ovitrap installation sites for gravid aedes aegypti in mirassol, state of são paulo, brazil. ovitraps were installed in ten houses per block over ten blocks. four ovitraps were placed per residence, one in the bedroom, one in the living room, and two outdoors with one in a sheltered area and one in an outside site. each week for eleven weeks, visits were made to examine the ovitraps and to change the paddles used for egg-laying. eggs were analyzed accordi ... | 2005 | 16113880 |
| suppression of brugia malayi (sub-periodic) larval development in aedes aegypti (liverpool strain) fed on blood of animals immunized with microfilariae. | preliminary studies were carried out to investigate the role of filarial specific antibodies, raised in an animal model against the filarial parasite, brugia malayi (sub-periodic), in blocking their early development in an experimental mosquito host, aedes aegypti (liverpool strain). in order to generate filarial specific antibodies, mongolian gerbils, meriones unguiculatus, were immunized either with live microfilariae (mf) of b. malayi or their homogenate. mf were harvested from the peritoneal ... | 2005 | 16113889 |
| monitoring of resistance to the pyrethroid cypermethrin in brazilian aedes aegypti (diptera: culicidae) populations collected between 2001 and 2003. | resistance to cypermethrin of different aedes aegypti brazilian populations, collected at two successive periods (2001 and 2002/2003), was monitored using the insecticide-coated bottles bioassay. slight modifications were included in the method to discriminate between mortality and the knock down effect. although this pyrethroid was recently started to be used in the country to control the dengue vector, a decrease in susceptibility was noted between both periods analyzed, particularly in the ci ... | 2005 | 16113895 |
| infestation of aedes aegypti estimated by oviposition traps in brazil. | to assess infestation levels of aedes aegypti using the oviposition trap (ovitrap) method and to compare these results with data obtained with the use of indices traditionally applied in public programs aimed at fighting this vector. | 2005 | 16113903 |
| [seasonal fluctuation of aedes aegypti in chaco province, argentina]. | to study the seasonal fluctuation of aedes aegypti and to correlate its abundance with climate conditions. | 2005 | 16113904 |
| asymmetrical competition and patterns of abundance of aedes albopictus and culex pipiens (diptera: culicidae). | we tested for competitive advantage among larvae of aedes albopictus (skuse) and culex pipiens l. in a laboratory experiment and determined the frequency and spatial and temporal patterns of co-occurrence in the field in east st. louis, il. in a laboratory competition experiment at multiple combined densities of ae. albopictus and cx. pipiens larvae, ae. albopictus survivorship and developmental times were significantly affected by conspecific densities but not by cx. pipiens densities. in contr ... | 2005 | 16119544 |
| genetic structure and gene flow among aedes aegypti (diptera: culicidae) populations from central thailand. | isozymes from five wild-caught populations of aedes aegypti (l.) were compared using starch gel electrophoresis to estimate rates of gene flow between and among geographically close mosquito populations. ae. aegypti were collected from five different locations in bangkok, thailand. one collection was obtained from central bangkok (huai-khwang); the other four samples were obtained from surrounding areas (districts latkrabang, ratburana, laksi, and bangkok noi). based on 24 loci (17 enzyme system ... | 2005 | 16119549 |
| potential use of pyriproxyfen for control of aedes aegypti (diptera: culicidae) in iquitos, perú. | the effects of pyriproxyfen were tested against a local population of aedes aegypti (l.) in iquitos, perú. bioassays showed that, when applied to late instars, pyriproxyfen prevented adult emergence at extremely low concentrations (lc50 = 0.012 ppb). there was no adult emergence from water sampled from storage tanks that had been seeded with the equivalent of 50-83 ppb (ai) pyriproxyfen. five months after treatment, despite constant dilution of these tanks, water sampled from these sources conti ... | 2005 | 16119551 |
| feeding deterrent effects of catnip oil components compared with two synthetic amides against aedes aegypti. | recently, catnip, nepeta cataria l. (lamiaceae), essential oil has been formulated and marketed as an alternative repellent for protection against biting arthropods by several vendors. we isolated the major active components of catnip oil, e,z- and z,e-nepetalactone, and quantitatively measured their antibiting efficacy compared with the repellents n,n-diethyl-3-methylbenzamide (deet) and chiral (1s,2's)-2-methylpiperidinyl-3-cyclohexene-1-carboxamide (ss220) against the yellowfever mosquito, ae ... | 2005 | 16119554 |
| characterization of n-linked oligosaccharides in chorion peroxidase of aedes aegypti mosquito. | a peroxidase is present in the chorion of aedes aegypti eggs and catalyzes chorion protein cross-linking during chorion hardening, which is critical for egg survival in the environment. the unique chorion peroxidase (cpo) is a glycoprotein. this study deals with the n-glycosylation site, structures, and profile of cpo-associated oligosaccharides using mass spectrometric techniques and enzymatic digestion. cpo was isolated from chorion by solubilization and several chromatographic methods. mono-s ... | 2005 | 16131661 |
| identification and characterization of the fibrinogen-like domain of fibrinogen-related proteins in the mosquito, anopheles gambiae, and the fruitfly, drosophila melanogaster, genomes. | the fibrinogen-like (fbg) domain, which consists of approximately 200 amino acid residues, has high sequence similarity to the c-terminal halves of fibrinogen beta and gamma chains. fibrinogen-related proteins (freps), which contain fbg domains in their c-terminal region, are found universally in vertebrates and invertebrates. in invertebrates, freps are involved in immune responses and other aspects of physiology. to understand the complexity of this family in insects, we analyzed freps in the ... | 2005 | 16150145 |
| transgenic alteration of toll immune pathway in the female mosquito aedes aegypti. | reverse genetics is a powerful tool for understanding gene functions and their interactions in the mosquito innate immunity. we took the transgenic approach, in combination with the rna interference (rnai) technique, to elucidate the role of mosquito rel1, a homolog of drosophila dorsal, in regulation of toll immune pathway in the mosquito aedes aegypti. by transforming the mosquitoes with deltarel1-a or a double-stranded rna construct of rel1 driven by the female fat body-specific vitellogenin ... | 2005 | 16157887 |
| dengue and dengue hemorrhagic fever epidemics in brazil: what research is needed based on trends, surveillance, and control experiences? | dengue epidemics account annually for several million cases and deaths worldwide. the high endemic level of dengue fever and its hemorrhagic form correlates to extensive domiciliary infestation by aedes aegypti and multiple viral serotype human infection. this study analyzed serial case reports registered in brazil since 1981, describing incidence evolutionary patterns and spatial distribution. epidemic waves followed the introduction of every serotype (den 1 to 3), and reduction in susceptible ... | 2005 | 16158135 |
| partial restoration of antibacterial activity of the protein encoded by a cryptic open reading frame (cyt1ca) from bacillus thuringiensis subsp. israelensis by site-directed mutagenesis. | insecticidal crystal proteins of bacillus thuringiensis belong to two unrelated toxin families: receptor-specific cry toxins against insects and cyt toxins that lyse a broad range of cells, including bacteria, via direct binding to phospholipids. a new cyt-like open reading frame (cyt1ca) encoding a 60-kda protein, has recently been discovered (c. berry et al., appl. environ. microbiol. 68:5082-5095, 2002). cyt1ca displays the structure of a two-domain fusion protein: the n-terminal moiety resem ... | 2005 | 16159771 |
| modulation of anopheles gambiae gene expression in response to o'nyong-nyong virus infection. | to determine if gene expression of an. gambiae is modulated in response to o'nyong-nyong virus (onnv) infection, we utilized cdna microarrays including about 20 000 cdnas. gene expression levels of onnv-infected female mosquitoes were compared to that of the uninfected control females harvested at 14 days postinfection. in response to onnv infection, expression levels of 18 genes were significantly modulated, being at least two-fold up- or down-regulated. quantitative real-time pcr analysis (qrt ... | 2005 | 16164603 |
| synergism between ammonia, lactic acid and carboxylic acids as kairomones in the host-seeking behaviour of the malaria mosquito anopheles gambiae sensu stricto (diptera: culicidae). | host odours play a major role in the orientation and host location of blood-feeding mosquitoes. anopheles gambiae giles sensu stricto, which is the most important malaria vector in africa, is a highly anthropophilic mosquito species, and the host-seeking behaviour of the females of this mosquito is guided by volatiles of human origin. ammonia, lactic acid and several carboxylic acids are known to be present in the human odour blend. we investigated the effect of these compounds on naive female m ... | 2005 | 15703334 |
| dengue and dengue hemorrhagic fever, brazil, 1981-2002. | in the last 5 years, brazil has accounted for approximately 70% of reported dengue fever cases in the americas. we analyzed trends of dengue and dengue hemorrhagic fever (dhf) from the early 1980s to 2002 by using surveillance data from the brazilian ministry of health. two distinct epidemiologic patterns for dengue were observed: localized epidemics (1986-1993), and endemic and epidemic virus circulation countrywide (1994-2002). currently, serotypes 1, 2, and 3 cocirculate in 22 of 27 states. d ... | 2005 | 15705322 |
| induction of nitric oxide synthase in anopheles stephensi by plasmodium falciparum: mechanism of signaling and the role of parasite glycosylphosphatidylinositols. | malaria parasite (plasmodium spp.) infection in the mosquito anopheles stephensi induces significant expression of a. stephensi nitric oxide synthase (asnos) in the midgut epithelium as early as 6 h postinfection and intermittently thereafter. this induction results in the synthesis of inflammatory levels of nitric oxide (no) in the blood-filled midgut that adversely impact parasite development. in mammals, p. falciparum glycosylphosphatidylinositols (pfgpis) can induce nos expression in immune ... | 2005 | 15845481 |
| characterization of an infectious clone of the wild-type yellow fever virus asibi strain that is able to infect and disseminate in mosquitoes. | infectious clone technology provides an opportunity to study the molecular basis of arthropod-virus interactions in detail. this study describes the development of an infectious clone of the prototype yellow fever virus asibi strain (yfv-as) with the purpose of identifying sequences or domains that influence infection dynamics in the mosquito vector. the full-length cdna of yfv-as virus was produced from rt-pcr products of parental viral rna. these were cloned into a low-copy-number plasmid prev ... | 2005 | 15914853 |
| bottle and biochemical assays on temephos resistance in aedes aegypti in thailand. | the bottle bioassay measuring the time-mortality rate is a simplified procedure for detecting insecticide resistance. it can be used with a biochemical microplate assay to identify the mechanism involved. this integrated approach was used to detect temephos resistance in aedes aegypti from nonthaburi (lowest use) and roi et (highest use). ae. aegypti bkk1 laboratory strain was used as the susceptible reference strain. the appropriate concentration of insecticide for bottle bioassay was determine ... | 2005 | 15916049 |
| differential identification of ascogregarina species (apicomplexa: lecudinidae) in aedes aegypti and aedes albopictus (diptera: culicidae) by polymerase chain reaction. | we report 2 polymerase chain reaction (pcr)-based methods for distinguishing morphologically similar gregarine species based on amplification of variable regions of the internal transcribed spacer region of ribosomal dna. the gregarines we investigated were ascogregarina barretti (vavra), a. culicis (ross), and a. taiwanensis (lien and levine), parasites of the mosquitoes ochlerotatus triseriatus (say), aedes aegypti (linnaeus), and ae. albopictus (skuse), respectively. these 3 important vector ... | 2005 | 16539016 |
| analyzing ligation mixtures using a pcr based method. | we have developed a simple and effective method (lig-pcr) for monitoring ligation reactions using pcr and primers that are common to many cloning vectors. ligation mixtures can directly be used as templates and the results can be analyzed by conventional gel electrophoresis. the pcr products are representative of the recombinant molecules created during ligation and the corresponding transformants. orientation of inserts can also be determined using an internal primer. the usefulness of this met ... | 2005 | 16136227 |
| an information value based analysis of physical and climatic factors affecting dengue fever and dengue haemorrhagic fever incidence. | vector-borne diseases are the most dreaded worldwide health problems. although many campaigns against it have been conducted, dengue fever (df) and dengue haemorrhagic fever (dhf) are still the major health problems of thailand. the reported number of dengue incidences in 1998 for the thailand was 129,954, of which sukhothai province alone reported alarming number of 682. it was the second largest epidemic outbreak of dengue after 1987. government arranges the remedial facilities as and when den ... | 2005 | 15943863 |
| immunostaining for allatotropin and allatostatin-a and -c in the mosquitoes aedes aegypti and anopheles albimanus. | confocal laser-scanning microscopy was used to carry out a comparative study of the immunostaining for three families of neuropeptides, viz., allatostatin-a (as-a), allatostatin-c (as-c) and allatotropin (at), in adult female mosquitoes of aedes aegypti and anopheles albimanus. the specific patterns of immunostaining for each of the three peptides were similar in both species. the antisera raised against at, as-a, and as-c revealed intense immunoreactivity in the cells of each protocerebral lobe ... | 2005 | 15909164 |
| rnai-induced silencing of embryonic tryptophan oxygenase in the pyralid moth, plodia interpunctella. | gene silencing through the introduction of double-stranded rna (rna interference, rnai) provides a powerful tool for the elucidation of gene function in many systems, including those where genomics and proteomics are incomplete. the use of rnai technology for gene silencing in lepidoptera has lacked significant attention compared to other systems. to demonstrate that rnai can be utilized in the lepidopteran, plodia interpunctella, we cloned a cdna for tryptophan oxygenase, and showed that silenc ... | 2005 | 15861231 |
| site-specific genomic targeting in drosophila. | transcriptional regulation of transgenes depends upon genomic localization in higher eukaryotes. for the applied use of transgenic organisms as producers of pharmaceutically relevant proteins or as pest population control agents, a method to make transgene expression predictable is highly desirable. a targeting method that allows precise cassette replacement comprising solely genes of interest (without extraneous donor vector sequences) would be highly advantageous for insects and other multicel ... | 2005 | 16116081 |
| synergistic action of e74b and ecdysteroid receptor in activating a 20-hydroxyecdysone effector gene. | a number of insect effector genes activated by the steroid hormone 20-hydroxyecdysone (20e) are dually controlled by the ecdysteroid receptor (ecr/usp) and products of ecdysteroid early responsive genes (e74, e75, and broad). however, the molecular mechanism of this dual action is poorly understood. here we examined transcriptional activation of the vitellogenin (vg) gene in the yellow fever mosquito, aedes aegypti, by ecr/usp and e74 in response to an elevation of 20e titers. there are two isof ... | 2005 | 16230625 |
| the shavenoid gene of drosophila encodes a novel actin cytoskeleton interacting protein that promotes wing hair morphogenesis. | the simple cellular composition and array of distally pointing hairs has made the drosophila wing a favored system for studying planar polarity and the coordination of cellular- and tissue-level morphogenesis. the developing hairs are filled with f-actin and microtubules and the activity of these cytoskeletons is important for hair morphogenesis. on the basis of mutant phenotypes several genes have been identified as playing a key role in stimulating hair formation. mutations in shavenoid (sha) ... | 2005 | 16322503 |
| ancestry and progeny of nutrient amino acid transporters. | the biosynthesis of structural and signaling molecules depends on intracellular concentrations of essential amino acids, which are maintained by a specific system of plasma membrane transporters. we identify a unique population of nutrient amino acid transporters (nats) within the sodium-neurotransmitter symporter family and have characterized a member of the nat subfamily from the larval midgut of the yellow fever vector mosquito, aedes aegypti (aeaat1, aar08269), which primarily supplies pheny ... | 2005 | 15665107 |
| nonstationary influence of el niño on the synchronous dengue epidemics in thailand. | several factors, including environmental and climatic factors, influence the transmission of vector-borne diseases. nevertheless, the identification and relative importance of climatic factors for vector-borne diseases remain controversial. dengue is the world's most important viral vector-borne disease, and the controversy about climatic effects also applies in this case. here we address the role of climate variability in shaping the interannual pattern of dengue epidemics. | 2005 | 15839751 |
| comparison of the properties of the five soluble guanylyl cyclase subunits in drosophila melanogaster. | the drosophila melanogaster genome contains 5 genes that code for soluble guanylyl cyclase subunits. two of these genes code for subunits, gycalpha-99b and gycbeta-100b, which form a conventional no-sensitive guanylyl cyclase and the other three code for atypical subunits, gyc-88e, gyc-89da and gyc-89db. the properties and distribution of gyc-88e and gyc-89db have previously been described and here gyc-89da is described. gyc-89da only forms an active guanylyl cyclase when co-expressed with gyc-8 ... | 2005 | 16341244 |
| eco-epidemiological analysis of dengue infection during an outbreak of dengue fever, india. | this study was designed to find out a relationship of dengue infection with climatic factors such as rainfall, temperature and relative humidity during the dengue fever epidemic in the year 2003. blood samples were collected from 1550 patients experiencing a febrile illness clinically consistent with dengue infection. serological confirmation of dengue infection was done using dengue duo igm and igg rapid strip test (pan bio, australia), which detected dengue-specific antibodies. monthly data of ... | 2005 | 15831102 |
| a spider that feeds indirectly on vertebrate blood by choosing female mosquitoes as prey. | spiders do not feed directly on vertebrate blood, but a small east african jumping spider (salticidae), evarcha culicivora, feeds indirectly on vertebrate blood by choosing as preferred prey female mosquitoes that have had recent blood meals. experiments show that this spider can identify its preferred prey by sight alone and by odor alone. when presented with two types of size-matched motionless lures, e. culicivora consistently chose blood-fed female mosquitoes in preference to nonmosquito pre ... | 2005 | 16217015 |
| oviposition deterrent and skin repellent activities of solanum trilobatum leaf extract against the malarial vector anopheles stephensi. | the leaf extract of solanum trilobatum (solanaceae) was tested under laboratory conditions for oviposition deterrent and skin repellent activities against the adult mosquito anopheles stephensi. concentrations of 0.01, 0.025, 0.05, 0.075 and 0.1% reduced egg laying by gravid females from 18 to 99% compared to ethanol-treated controls. in skin repellent tests, concentrations of 0.001, 0.005, 0.01, 0.015, and 0.02 % provided 70 to 120 minutes protection against mosquito bites, whereas the ethanol ... | 2005 | 16341247 |
| prevalence-dependent costs of parasite virulence. | costs of parasitism are commonly measured by comparing the performance of infected groups of individuals to that of uninfected control groups. this measure potentially underestimates the cost of parasitism because it ignores indirect costs, which may result from the modification of the competitiveness of the hosts by the parasite. in this context, we used the host-parasite system consisting of the yellow fever mosquito aedes aegypti and the microsporidian parasite vavraia culicis to address this ... | 2005 | 16008503 |
| antiviral silencing in animals. | rna silencing or rna interference (rnai) refers to the small rna-guided gene silencing mechanism conserved in a wide range of eukaryotic organisms from plants to mammals. as part of this special issue on the biology, mechanisms and applications of rnai, here we review the recent advances on defining a role of rnai in the responses of invertebrate and vertebrate animals to virus infection. approximately 40 mirnas and 10 rnai suppressors encoded by diverse mammalian viruses have been identified. a ... | 2005 | 16154568 |
| dengue fever: new paradigms for a changing epidemiology. | dengue is the most important arthropod-borne viral disease of public health significance. compared with nine reporting countries in the 1950s, today the geographic distribution includes more than 100 countries worldwide. many of these had not reported dengue for 20 or more years and several have no known history of the disease. the world health organization estimates that more than 2.5 billion people are at risk of dengue infection. first recognised in the 1950s, it has become a leading cause of ... | 2005 | 15743532 |
| a new larvicidal lignan from piper fimbriulatum. | a new lignan, 3,4,5'-trimethoxy-3',4'-methylenedioxy-7,9':7',9 diepoxylignan (1) (6-[4-(3,4-dimethoxy-phenyl)-tetrahydro-furo[3,4-c.]furan-1-yl]-4-methoxy-benzo[ ] dioxole) together with two known lignans, 7'-epi.-sesartemin (2) and diayangambin (3), and a known flavonoid, 5-hydroxy-7,4'-dimethoxyflavone (4), were isolated from the leaves of piper fimbriulatum. c. dc. their structures were assigned by a combination of one- and two-dimensional nmr techniques. 7'-epi.-sesartemin (2) showed the hig ... | 2005 | 28925841 |
| insight into a conserved lifestyle: protein-carbohydrate adhesion strategies of vector-borne pathogens. | 2005 | 16299269 | |
| neurologic aspects of infections in international travelers. | as international travel for business and pleasure becomes part of contemporary lifestyle, the clinician today is confronted with an increasing number of travelers returning ill with unfamiliar syndromes. the physician will encounter a myriad of patients with exotic infections, emerging infectious diseases, or resurgent old-world infections. | 2005 | 15631642 |
| [a histological study of pathological changes in aedes aegypti l. larvae experimentally infected with mosquito densonucleosis virus]. | 2005 | 1214642 | |
| comparison of phospholipid composition of aedes aegypti and aedes albopictus cells obtained from logarithmic and stationary phases of growth. | aedes aegypti and aedes albopictus cells were grown in tissue culture and harvested at logarithmic and stationary phases of development. the phospholipids were extracted, separated into lipid classes, and fatty acid composition of each fraction determined. the phosphatidylethanolamine fraction was the major lipid (42-54%). with aging, the a. aegypti cells showed an increase in polyenes in the phosphatidylcholine and phosphatidylethanolamine fractions and in monoenes and polyenes in the phosphati ... | 2005 | 1196018 |
| [experimental use of an insecticide with a ddt base on haitian strain of aedes aegypti & its comparison with strains from other sources]. | 2005 | 13429991 | |
| [comparative effects of several insecticides of residual effect on aedes aegypti and culex fatigans]. | 2005 | 13341122 | |
| laboratory study on larvicidal properties of leaf extract of calotropis procera (family-asclepiadaceae) against mosquito larvae. | fresh leaf extract of milkweed (calotropis procera) showed larvicidal properties against mosquito larvae of anopheles stephensi, culex quinquefasciatus and aedes aegypti. methanolic extracts of the same plant were, however, more effective as larvicide. c. procera is a weed growing in uncultivated soils as well as in dry, arid zones in indian subcontinent throughout the year. the results of this study suggest the utility of milkweed as potential technology for control of mosquito larvae. | 2005 | 16749273 |
| meconial peritrophic matrix structure, formation, and meconial degeneration in mosquito pupae/pharate adults: histological and ultrastructural aspects. | the noncellular peritrophic matrix (pm) that forms around the food bolus in the midgut of many arthropod species may influence the fate of ingested microbes. in mosquitoes, pms have been identified in the pupal as well as larval and adult stages. in pupae, the pms surround the meconium, the sloughed larval midgut epithelium. meconial pm1 (mpm1) forms early in the pupal stadium, and a second meconial pm (mpm2) sometimes forms around the time of adult emergence. a recent study suggests that mpms c ... | 2005 | 16465731 |
| a novel high-throughput screening system to evaluate the behavioral response of adult mosquitoes to chemicals. | a modular and novel assay system for rapid mass screening of chemical compounds for contact irritant and spatial repellent actions against adult mosquitoes is described. the responses of aedes aegypti to various concentrations of 3 topical repellents, deet, bayrepel, and ss220, were evaluated. at treatment concentrations > or = 25 nmol/cm2 of ss220, mosquitoes exhibited significant contact irritant (escape) and spatial repellent (movement away from the chemical source) responses, whereas, a 10-f ... | 2005 | 16506566 |
| bruchid (coleoptera: bruchidae) ovicidal phenylbutanoid from zingiber purpureum. | the larvicidal activity of the dichloromethane extract of zingiber purpureum roscoe (zingiberaceae) rhizome against the second instar of aedes aegypti (l.) (diptera: culicidae) is shown to be due to 4-(3',4'-dimethoxyphenyl)buta-1,3-diene. the diene also showed ovicidal activity against the bruchid callosobruchus maculatus (f.) (coleoptera: bruchidae). most of the eggs laid by bruchids on treated cowpea seeds were transparent, and very few of them contained developing embryos. the few larvae pro ... | 2005 | 16156567 |
| novel glycosidic linkage in aedes aegypti chorion peroxidase: n-mannosyl tryptophan. | aedes aegypti chorion peroxidase (cpo) plays a crucial role in chorion hardening by catalyzing chorion protein cross-linking through dityrosine formation. the enzyme is extremely resistant to denaturing conditions, which seem intimately related to its post-translational modifications, including disulfide bond formation and glycosylation. in this report, we have provided data that describe a new type of glycosylation in cpo, where a mannose is linked to the n-1 atom of the indole ring of trp resi ... | 2005 | 16150691 |
| mathematical model to assess the control of aedes aegypti mosquitoes by the sterile insect technique. | we propose a mathematical model to assess the effects of irradiated (or transgenic) male insects introduction in a previously infested region. the release of sterile male insects aims to displace gradually the natural (wild) insect from the habitat. we discuss the suitability of this release technique when applied to peri-domestically adapted aedes aegypti mosquitoes which are transmissors of yellow fever and dengue disease. | 2005 | 16125739 |
| the drosophila melanogaster cinnabar gene is a cell autonomous genetic marker in aedes aegypti (diptera: culicidae). | the cinnabar gene of drosophila melanogaster (meigen) encodes for kynurenine hydroxylase, an enzyme involved in ommochrome biosynthesis. this gene is commonly included as a visible genetic marker in gene vectors used to create transgenic aedes aegypti (l.) that are homozygous for the khw allele, the mosquito homolog of cinnabar. unexpectedly, the phenotype of cells expressing kynurenine hydroxylase in transgenic ae. aegypti is cell autonomous as demonstrated by the recovery of insects heterozygo ... | 2005 | 16119567 |