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lithium: long-term effects on the kidney--ii. structural changes.forty-six patients treated with lithium for an average of 8 yr participated in a follow-up study involving a kidney biopsy. the results were compared with renal biopsy specimens from an age-matched group of controls never treated with lithium. the average number of totally scelerotic glomeruli and atrophic tubuli was higher in lithium-treated patients. the histopathological changes showed significant correlations with lithium dosage schedule. both the proportions of sclerotic glomeruli, atrophic ...20133681762
new liability insurance program to cover most nps. 20133650032
wide complex tachycardia: diagnosis and treatment. 20133647985
[presentation of the front]. 20133635982
the association of femoral retroversion with slipped capital femoral epiphysis. 20133571323
hemangiomas in infancy and childhood. 20133562091
changing concepts of childhood in society. 20133641179
metacarpal thickness in south african population. 20133672283
teaching and credentialing the physically challenged: state of the art. a review of change in the clinical and scientific data since 1980.more than 30 million americans are disabled. wide experience has shown that these conditions do not prevent these individuals from becoming proficient in the knowledge and skills of cpr. instructional materials and methods can be modified to help this special population learn cpr despite handicaps. the american heart association has supported these special efforts since 1978, but no comprehensive resource exists for cpr instructors interested in helping the "physically challenged" individual lea ...20133536165
overuse injuries.stress injuries common to running athletes are always associated with a breakdown of the protective mechanisms normally inherent in healthy posture and gait. it is more important to identify and treat these breakdowns of the motor process than the resulting injury itself. simple motor screening of athletes can identify functional abnormalities that lead to stress injuries. this screen includes assessment of flexibility of all major joints, responsiveness of primary postural muscles, posture, bal ...20133893750
posterior cerebral artery stenosis.we analyzed the clinical features of symptomatic posterior cerebral artery (pca) stenosis in 6 patients selected from 15 patients with angiographically documented pca atherostenosis occurring during a 7-year period. transient ischemic attacks (tias) were the major presentation in 5 patients. a homonymous visual field defect was present in 2 patients. tia symptoms were predominantly visual or sensory, or both. the most common visual symptom was difficulty seeing to one side. one patient saw flash ...20133827217
[the incidence of palmar erythema in patients with alcoholic fatty liver--a comparative study with fatty liver of other origins]. 20133820761
dermatologic radiotherapy. the risk-benefit ratio. 20133789771
an ideal agent for drawover anaesthesia? 20133789382
[immediate and long-term results of endarterectomies in atherosclerotic occlusions of the abdominal aorta and iliac arteries].an analysis of the clinical material evidences a sufficiently high efficiency of endarterectomy even in spread occlusions of the aorto-femoral segments of atherosclerotic etiology with the absence of atheromatosis and calcinosis of the arteries. good and satisfactory immediate results were obtained in 89.4% of the patients, long-term results in 52.8% and with regard to reoperations--in 67.5% of the patients.20133672780
a hospital's on-site child care center proves to make business sense.on-site child care centers in hospitals can improve productivity and reduce absenteeism, tardiness, and turnover. they are also effective recruitment tools and can improve the quality of work performed.20133733441
enrollment update: comparison of fall 1984 and 1985 enrollments in american association of colleges of nursing member schools and with trends in higher education enrollments. 20133633928
outcome of treatment at a psycho-educational day unit for young children.the outcome of treatment at the psychiatric day centre of the red cross war memorial children's hospital in cape town is described. forty-five children were followed up for 2-6 years after discharge--12 were asymptomatic, 22 moderately improved and 11 unimproved. possible means of improving outcome are discussed.20133672274
[what does family anamnesis contribute in routine medical practice?]. 20133557251
continuing problem in congenital rubella syndrome in southern japan. its outbreak in fukuoka and the surrounding areas after the 1965-1969 and 1975-1977 rubella epidemics. 20133758900
[photoelastic analysis of stress on alveolar bone in the abutment of the fixed bridge under vertical loading]. 20133542798
[liver diseases and glycoregulation]. 20133811778
characteristics and motives of women choosing elective induction of labour.personal characteristics of healthy term pregnant women who chose elective induction or spontaneous onset of labour and the motives for their choice were assessed. almost 50% of 237 women with uncomplicated pregnancies opted for elective induction when offered the opportunity. these women appeared to have had more complaints during their pregnancy and menstrual periods, more complications in their obstetrical history and to be more anxious about their labour than women who chose a spontaneous on ...20133735181
basal cell epithelioma with giant tumor cells: light and electron microscopic study.a case of basal cell epithelioma (bce) with multiple large nuclei on the right buttock of a 69-year-old woman is reported, and the skin was studied by light and electron microscopy. this represented the only case (0.85%) of giant tumor cells with multiple nuclei among 117 patients with bce. ultrastructural studies revealed that giant tumor cells were not different from ordinary tumor cells of bce, except for large convoluted nuclei. in the cytoplasms of giant tumor cells, we observed autophagic ...20133624586
[intracranial hemorrhage in full-term neonates]. 20133820717
aerosols in chronic airway obstruction. 20133748105
[functional state of body systems in local hyperthermia].the bioelectrical activity of the cerebral cortex, cardiovascular system status, cell-mediated immunity and peripheral blood parameters were studied in 208 cases of head and neck cancer who received local pneumo- and ultra-high frequency hyperthermia in combination with chemoradiation treatment. local hyperthermia was found to produce no untoward side-effects.20133788085
electrical therapy for cardiovascular emergencies.this presentation summarizes advances in electrical therapy of cardiovascular emergencies. the urgency of delivering definitive therapy is emphasized, and the roles of automatic internal and external defibrillators, practical external pacing, and mechanical techniques for cardioversion and defibrillation are evaluated. standard position of the electroplates is recommended except in patients who have permanently implanted pacemakers. in these patients, the defibrillation electrodes should be at l ...20133536157
fatal atypical o:3 yersinia pseudotuberculosis infection in cynomolgus macaques.fatal yersinia pseudotuberculosis infection in cynomolgus macaques was diagnosed based upon pathology, microbiology and pcr for this study. pathological findings included acute, erosive to ulcerative, necrohemorrhagic enterocolitis. genotyping by pcr showed an o:3 pattern (gmd-fcl(+), ddhc-prt(+), manb(+), ddha-b(+)), but an additional gene, wbyk, was detected. this is the second report to identify wbyk+ o:3 genotype as the cause of fatal yersiniosis. the first case was reported in 2008, and inv ...201323932075
alternative sigma factor σe has an important role in stress tolerance of yersinia pseudotuberculosis ip32953.yersinia pseudotuberculosis is an important pathogen that probably survives well in the modern food chain. however, little is known about the mechanisms that allow the growth of this pathogen in foods under stress conditions. the expression of rpoe encoding σ(e) was defined by quantitative real-time reverse transcription-pcr. expression of rpoe was induced at 3°c, 37°c, and 42°c, under exposure to 3% nacl, 3% ethanol, or high and low ph, in relation to its expression at the optimum growth temper ...201323872565
draft genome sequences of two yersinia pseudotuberculosis st43 (o:1b) strains, b-7194 and b-7195.we report the first draft genome sequences of two yersinia pseudotuberculosis sequence type 43 (st43) (o:1b) strains, b-7194 and b-7195, isolated in russia. the total lengths of the assemblies are 4,427,121 bp and 4,608,472 bp, and 3,819 and 4,018 coding sequences, respectively, were predicted within the genomes.201323868131
expression of a yersinia pseudotuberculosis type vi secretion system is responsive to envelope stresses through the ompr transcriptional activator.the type vi secretion system (t6ss) is a macromolecular complex widespread in gram-negative bacteria. although several t6ss are required for virulence towards host models, most are necessary to eliminate competitor bacteria. other functions, such as resistance to amoeba predation, biofilm formation or adaptation to environmental conditions have also been reported. this multitude of functions is reflected by the large repertoire of regulatory mechanisms shown to control t6ss expression, productio ...201323840509
adhesins and host serum factors drive yop translocation by yersinia into professional phagocytes during animal infection.yersinia delivers yops into numerous types of cultured cells, but predominantly into professional phagocytes and b cells during animal infection. the basis for this cellular tropism during animal infection is not understood. this work demonstrates that efficient and specific yop translocation into phagocytes by yersinia pseudotuberculosis (yptb) is a multi-factorial process requiring several adhesins and host complement. when wt yptb or a multiple adhesin mutant strain, δailδinvδyada, colonized ...201323818844
effect of phenol-induced changes in lipid composition on conformation of ompf-like porin of yersinia pseudotuberculosis.the present work aimed to compare the effects of different lysophosphatidylethanolamine (lpe) content in lipids derived from yersinia pseudotuberculosis cells exposed and not exposed to phenol on the conformation of ompf-like porin of these bacteria. differential scanning calorimetry and intrinsic protein fluorescence showed that the 2.5-fold increase of lpe content and the corresponding increase in the phase transition temperature of bacterial lipids were accompanied by enhanced protein thermos ...201323742936
solution of the problem of nonspecific binding in solid-phase noninstrumental dot immunoassay.in this work, we evaluated blocking properties of various reagents used in dot immunoassay test systems on nitrocellulose membranes employing carbon-based diagnosticum to determine antibodies to tetanus anatoxin, thermostable toxin of yersinia pseudotuberculosis, and hiv-i. the results support the usage of a nonionic detergent tween 20 which efficiently inhibited nonspecific protein binding to the solid-phase reagent surface and did not decrease sensitivity of the analysis.201323657647
demarcating sura activities required for outer membrane targeting of yersinia pseudotuberculosis adhesins.sura is a periplasmic protein folding factor involved in chaperoning and trafficking of outer membrane proteins across the gram-negative bacterial periplasm. in addition, sura also possesses peptidyl-prolyl cis/trans isomerase activity. we have previously reported that in enteropathogenic yersinia pseudotuberculosis, sura is needed for bacterial virulence and envelope integrity. in this study, we investigated the role of sura in the assembly of important yersinia adhesins. using genetic mutation ...201323589578
draft genome sequences of five yersinia pseudotuberculosis st19 isolates and one isolate variant.we report the first draft genome sequences of five yersinia pseudotuberculosis isolates of sequence type (st) 19 and of a variant from one of the five isolates. the total length of assemblies ranged from 4,226,485 bp to 4,274,148 bp, including between 3,808 and 3,843 predicted coding sequences.201323580708
impact of host membrane pore formation by the yersinia pseudotuberculosis type iii secretion system on the macrophage innate immune response.type iii secretion systems (t3sss) are used by gram-negative pathogens to form pores in host membranes and deliver virulence-associated effector proteins inside host cells. in pathogenic yersinia, the t3ss pore-forming proteins are yopb and yopd. mammalian cells recognize the yersinia t3ss, leading to a host response that includes secretion of the inflammatory cytokine interleukin-1β (il-1β), toll-like receptor (tlr)-independent expression of the stress-associated transcription factor egr1 and t ...201323297383
sequencing of virulence genes shows limited genetic variability in yersinia pseudotuberculosis.yersinia pseudotuberculosis is a foodborne pathogen often detected and identified using polymerase chain reaction (pcr) with primers targeted to virulence genes. sequence variability of the virulence genes in strains representing different serotypes is unknown. sequence variability could hinder the recognition of this pathogen by pcr and affect the host-pathogen interactions. sequencing of inv, virf, and yada of 18 y. pseudotuberculosis strains showed limited variability of inv and virf, whereas ...201323153288
a type vi secretion system regulated by ompr in yersinia pseudotuberculosis functions to maintain intracellular ph homeostasis.type vi secretion systems (t6sss) which widely distributed in gram-negative bacteria have been primarily studied in the context of cell interactions with eukaryotic hosts or other bacteria. we have recently identified a thermoregulated t6ss4 in the enteric pathogen yersinia pseudotuberculosis. here we report that ompr directly binds to the promoter of t6ss4 operon and regulates its expression. further, we observed that the ompr-regulated t6ss4 is essential for bacterial survival under acidic con ...201323094603
role of yopk in yersinia pseudotuberculosis resistance against polymorphonuclear leukocyte defense.the enteropathogen yersinia pseudotuberculosis can survive in the harsh environment of lymphoid compartments that abounds in immune cells. this capacity is dependent on the plasmid-encoded yersinia outer proteins (yops) that are delivered into the host cell via a mechanism involving the yersinia type iii secretion system. we show that the virulence protein yopk has a role in the mechanism by which y. pseudotuberculosis avoids the polymorphonuclear leukocyte or neutrophil (pmn) defense. a yopk mu ...201323090955
the o-specific polysaccharide structure and gene cluster of serotype o:12 of the yersinia pseudotuberculosis complex, and the identification of a novel l-quinovose biosynthesis gene.a major virulence factor for yersinia pseudotuberculosis is lipopolysaccharide, including o-polysaccharide (ops). currently, the ops based serotyping scheme for y. pseudotuberculosis includes 21 known o-serotypes, with genetic and structural data available for 17 of them. the completion of the ops structures and genetics of this species will enable the visualization of relationships between o-serotypes and allow for analysis of the evolutionary processes within the species that give rise to new ...201323077132
right hemicolectomy for intestinal obstruction due to yersinia pseudotuberculosis: a histopathological surprise.a 56-year-old man was diagnosed to be having enteric fever elsewhere and put on antibiotics. despite therapy over 3 months, this patient had recurrent bouts of fever with chills, tiredness, and lower abdominal pain, distension, and vomiting with which he was admitted to our hospital. ct abdomen and pelvis showed significant wall thickening in the cecum and terminal ileum, with marked luminal narrowing and marked dilatation of proximal small bowel loops. clinically a diagnosis of intestinal obstr ...201324426569
[the bacteriophages yersinia pseudotuberculosis: the detection in strains of different o-serovars and their identification].the sample included five indicator pseudotuberculosis strains. the application of these strains permitted to isolate out of 161 strains of y. pseudotuberculosis 9 bacteriophages identical by their morphologic and serologic characteristics but having individual particularities in their lytic activity. the test on sensitivity to bacteriophages can be used in laboratory diagnostic to differentiate the strains of yersinia pseudotuberculosis.201324340749
molecular typing of yersinia pseudotuberculosis strains isolated from livestock in brazil.yersinia pseudotuberculosis can infect a broad range of animals. in brazil, this bacterium has been isolated from healthy and sick animals from sporadic cases and outbreaks of hemorrhagic gastroenteritis among livestock. however, the molecular diversity of these isolates is little understood. in this study, we used multilocus sequence typing, enterobacterial repetitive intergenic consensus pcr and pulsed-field gel electrophoresis to genotype 40 y. pseudotuberculosis strains belonging to bio-sero ...201324301748
genotyping of outbreak-associated and sporadic yersinia pseudotuberculosis strains by novel multilocus variable-number tandem repeat analysis (mlva).yersinia pseudotuberculosis human infections caused by serotype o:1 and o:3 isolates have been common in finland and have also caused outbreaks. epidemiological studies on the outbreaks have been limited by the lack of accurate typing methods. during the recent years, multilocus variable-number tandem repeat analysis (mlva) has been successfully applied for molecular typing of several bacterial pathogens. we designed an mlva scheme based on seven loci for y. pseudotuberculosis. the method was ab ...201324050949
molecular analysis of a novel toll/interleukin-1 receptor (tir)-domain containing virulence protein of y. pseudotuberculosis among far east scarlet-like fever serotype i strains.pathogenicity of yersinia pseudotuberculosis is determined by an arsenal of virulence factors. particularly, the yersinia outer proteins (yops) and the type iii secretion system (t3ss) encoded on the pyv virulence plasmid are required for yersinia pathogenicity. a specific group of y. pseudotuberculosis, responsible for the clinical syndrome described as far east scarlet-like fever (feslf), is known to have an altered virulence gene cluster. far east strains cause unique clinical symptoms for wh ...201324018301
the cytotoxic necrotizing factor of yersinia pseudotuberculosis (cnfy) enhances inflammation and yop delivery during infection by activation of rho gtpases.some isolates of yersinia pseudotuberculosis produce the cytotoxic necrotizing factor (cnfy), but the functional consequences of this toxin for host-pathogen interactions during the infection are unknown. in the present study we show that cnfy has a strong influence on virulence. we demonstrate that the cnfy toxin is thermo-regulated and highly expressed in all colonized lymphatic tissues and organs of orally infected mice. most strikingly, we found that a cnfy knock-out variant of a naturally t ...201324244167
nonenzymatic test system for noninstrumental determination of antibodies to species-specific fibers of yersinia pseudotuberculosis on the basis of nanosized carbon particles. 201323975407
outbreak of yersiniosis in egyptian rousette bats (rousettus aegyptiacus) caused by yersinia pseudotuberculosis serotype 4b.this report describes an outbreak of yersiniosis in egyptian rousette bats (rousettus aegyptiacus) caused by yersinia pseudotuberculosis serotype 4b. twelve of 61 bats died between november and december 2008 or in may 2009. the bats often displayed multiple yellow-white nodules in the spleen and liver. microscopically, these consisted of focal necrosis accompanied by inflammatory cell infiltration and colonies of gram-negative bacilli. the bacterial colonies were identified immunohistochemically ...201322980777
prevalence and genetic diversity of enteropathogenic yersinia spp. in pigs at farms and slaughter in lithuania.the prevalence of enteropathogenic yersinia spp. in pigs at farms and slaughter in relation to potential farming risk factors in lithuania was examined. pig faeces and carcase swab samples from 11 farms were studied at slaughterhouses. nine of the 11 farms were visited again 3-5 months later, and pooled feacal samples and environmental samples were collected. pathogenic yersinia enterocolitica was found in 64% and yersinia pseudotuberculosis in 45% of the sampled pig farms. all obtained isolates ...201323102547
proposed reclassification of pasteurella lymphangitidis sneath & stevens 1990 as yersinia pseudotuberculosis.the 16s rrna gene sequences of pasteurella lymphangitidis, yersinia pseudotuberculosis and yersinia pestis were found to be identical and multilocus sequence analysis could not discriminate between the three species. the susceptibility to a y. pseudotuberculosis phage and the presence of the y. pseudotuberculosis-specific invasin gene in p. lymphangitidis indicate that the latter should be reclassified as y. pseudotuberculosis.201323919959
proteolytic processing of the yersinia pestis yapg autotransporter by the omptin protease pla and the contribution of yapg to murine plague pathogenesis.autotransporter protein secretion represents one of the simplest forms of secretion across gram-negative bacterial membranes. once secreted, autotransporter proteins either remain tethered to the bacterial surface or are released following proteolytic cleavage. autotransporters possess a diverse array of virulence-associated functions such as motility, cytotoxicity, adherence and autoaggregation. to better understand the role of autotransporters in disease, our research focused on the autotransp ...201323657527
counting small rna in pathogenic bacteria.here, we present a modification to single-molecule fluorescence in situ hybridization that enables quantitative detection and analysis of small rna (srna) expressed in bacteria. we show that short (~200 nucleotide) nucleic acid targets can be detected when the background of unbound singly dye-labeled dna oligomers is reduced through hybridization with a set of complementary dna oligomers labeled with a fluorescence quencher. by neutralizing the fluorescence from unbound probes, we were able to s ...201323577771
application of chromosomal dna and protein targeting for the identification of yersinia pestis.a comprehensive strategy was developed and validated for the identification of pathogens from closely related near neighbors using both chromosomal and protein biomarkers, with emphasis on distinguishing yersinia pestis from the ancestral bacterium yersinia pseudotuberculosis.201323436733
inheritance of the lysozyme inhibitor ivy was an important evolutionary step by yersinia pestis to avoid the host innate immune response.yersinia pestis (the plague bacillus) and its ancestor, yersinia pseudotuberculosis (which causes self-limited bowel disease), encode putative homologues of the periplasmic lysozyme inhibitor ivy and the membrane-bound lysozyme inhibitor mlic. the involvement of both inhibitors in virulence remains subject to debate.201323402825
identification and characterization of small rnas in yersinia pestis.yersinia pestis, the etiologic agent of plague, is closely related to yersinia pseudotuberculosis evolutionarily but has a very different mode of infection. the rna-binding regulatory protein, hfq, mediates regulation by small rnas (srnas) and is required for virulence of both y. pestis and y. pseudotuberculosis. moreover, hfq is required for growth of y. pestis, but not y. pseudotuberculosis, at 37°c. together, these observations suggest that srnas play important roles in the virulence and surv ...201323324607
a multi-omic systems approach to elucidating yersinia virulence mechanisms.the underlying mechanisms that lead to dramatic differences between closely related pathogens are not always readily apparent. for example, the genomes of yersinia pestis (yp) the causative agent of plague with a high mortality rate and yersinia pseudotuberculosis (ypt) an enteric pathogen with a modest mortality rate are highly similar with some species specific differences; however the molecular causes of their distinct clinical outcomes remain poorly understood. in this study, a temporal mult ...201323147219
customizable pcr-microplate array for differential identification of multiple pathogens.customizable pcr-microplate arrays were developed for the rapid identification of salmonella typhimurium, salmonella saintpaul, salmonella typhi, shigella dysenteriae, escherichia coli o157:h7, francisella tularensis subsp. tularensis, francisella tularensis subsp. novicida, vibrio cholerae, vibrio parahaemolyticus, yersinia pestis, and yersinia pseudotuberculosis. previously, we identified highly specific primers targeting each of these pathogens. here, we report the development of customizable ...201324215700
physiological levels of glucose induce membrane vesicle secretion and affect the lipid and protein composition of yersinia pestis cell surfaces.yersinia pestis grown with physiologic glucose increased cell autoaggregation and deposition of extracellular material, including membrane vesicles. membranes were characterized, and glucose had significant effects on protein, lipid, and carbohydrate profiles. these effects were independent of temperature and the biofilm-related locus pgm and were not observed in yersinia pseudotuberculosis.201323686263
[effect of smoking on pregnancy]. 20133623251
[minor surgical procedures and bronchologic cleansing methods in the treatment of acute abscesses and gangrene of the lungs].easy means of "minor surgery" and bronchological methods of sanitation were used in 234 patients with acute purulent diseases with the aim of drainage and sanitation of purulent cavities and for bringing antibiotics to the pathological focus in addition to detoxification, stimulating and antibacterial therapy. it reduced lethality among these patients and restricted indications to radical operations.20133705313
economics and hospital information systems. 20133715673
allergens in hymenoptera venom. xvii. allergenic components of solenopsis invicta (imported fire ant) venom.pure venom from solenopsis invicta was collected by having the insects sting into a capillary tube. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) demonstrated that three major protein bands were present in the venom. a prototype commercial extract was compared and found to contain the three major venom proteins and additional components as well as high potency and specificity by rast. immunoblot studies were performed with sera from allergic individuals with blots prepared ...20133624682
the wzy o-antigen polymerase of yersinia pseudotuberculosis o:2a has a dependence on the wzz chain-length determinant for efficient polymerization.lipopolysaccharide is a major immunogenic structure for the pathogen yersinia pseudotuberculosis, which contains the o-specific polysaccharide (ops) that is presented on the cell surface. the ops contains many repeats of the oligosaccharide o-unit and exhibits a preferred modal chain length that has been shown to be crucial for cell protection in yersinia. it is well established that the wzz protein determines the preferred chain length of the ops, and in its absence, the polymerization of o uni ...201324164168
microbiological quality of fresh-cut carrots and process waters.fresh vegetables may be contaminated by pathogens in different ways after harvest. pathogenic microorganisms associated with fresh vegetables can cause severe outbreaks of foodborne disease. we discuss here the results of microbiological analysis of carrot samples, as well as of washing, processing, and wastewater samples. washed, unpeeled carrots generally contained the highest aerobic plate counts (mean, 5.5 log cfu/g). escherichia coli was not detected in any carrot or water sample examined. ...201323834799
patterning bacterial communities on epithelial cells.micropatterning of bacteria using aqueous two phase system (atps) enables the localized culture and formation of physically separated bacterial communities on human epithelial cell sheets. this method was used to compare the effects of escherichia coli strain mg1655 and an isogenic invasive counterpart that expresses the invasin (inv) gene from yersinia pseudotuberculosis on the underlying epithelial cell layer. large portions of the cell layer beneath the invasive strain were killed or detached ...201323785519
human single-chain urokinase is activated by the omptins pgte of salmonella enterica and pla of yersinia pestis despite mutations of active site residues.fibrinolysis is important in cell migration and tightly regulated by specific inhibitors and activators; of the latter, urokinase (upa) associates with enhancement of cell migration. active upa is formed through cleavage of the single-chain upa (scupa). the salmonella enterica strain 14028r cleaved human scupa at the peptide bond lys158-ile159, the site cleaved also by the physiological activator human plasmin. the cleavage led to activation of scupa, while no cleavage or activation were detecte ...201323763588
[physicochemical properties and antiproliferative activity of recombinant yersinia pseudotuberculosis l-asparaginase].the physicochemical, catalytic, and antiproliferative activity of a recombinant l-asparaginase from yersinia pseudotuberculosis (ypa) have been studied. the following results were obtained: the k(m) value for l-asparagine is 17 +/- 0.9 microm, the optimal temperature is 60 degrees c, ph is 8.0, pi is 5.4 +/- 0.3, the l-glutaminase activity is no more than 5-6% of the l-asparaginase activity, and the antiproliferative activity on the fisher l5178y lymphadenosis cell line comprised t/c = 136% (p < ...201323662446
expression and cytoprotective activity of the small gtpase rhob induced by the escherichia coli cytotoxic necrotizing factor 1.rhob is the only member of the rho subfamily of small gtpases, which is classified as an immediate early gene product. rhob is up-regulated in response to growth factors as well as cytotoxic and genotoxic agents. clostridial glucosylating toxins have been reported to evoke pronounced rhob expression, based on the inactivation of rho/ras proteins. in this study, we report on a long lasting expression of rhob in cultured cells upon activation of rho proteins by the cytotoxic necrotizing factor 1 ( ...201323732113
the resveratrol tetramer (-)-hopeaphenol inhibits type iii secretion in the gram-negative pathogens yersinia pseudotuberculosis and pseudomonas aeruginosa.society faces huge challenges, as a large number of bacteria have developed resistance towards many or all of the antibiotics currently available. novel strategies that can help solve this problem are urgently needed. one such strategy is to target bacterial virulence, the ability to cause disease e.g., by inhibition of type iii secretion systems (t3sss) utilized by many clinically relevant gram-negative pathogens. many of the antibiotics used today originate from natural sources. in contrast, m ...201324324737
genetically engineered frameshifted yopn-tyea chimeras influence type iii secretion system function in yersinia pseudotuberculosis.type iii secretion is a tightly controlled virulence mechanism utilized by many gram negative bacteria to colonize their eukaryotic hosts. to infect their host, human pathogenic yersinia spp. translocate protein toxins into the host cell cytosol through a preassembled ysc-yop type iii secretion device. several of the ysc-yop components are known for their roles in controlling substrate secretion and translocation. particularly important in this role is the yopn and tyea heterodimer. in this stud ...201324098594
caspase-11 activation in response to bacterial secretion systems that access the host cytosol.inflammasome activation is important for antimicrobial defense because it induces cell death and regulates the secretion of il-1 family cytokines, which play a critical role in inflammatory responses. the inflammasome activates caspase-1 to process and secrete il-1β. however, the mechanisms governing il-1α release are less clear. recently, a non-canonical inflammasome was described that activates caspase-11 and mediates pyroptosis and release of il-1α and il-1β. caspase-11 activation in response ...201323762026
[prof. aleksandr grigor'evich eletskiĭ (on the centenary of his birth)]. 20133887276
pentose-phosphate shunt activity and red blood cell malonyldialdehyde levels in haemodialysis and in capd patients.the baseline concentrations of mda in the rbc membranes and the mda production in the erythrocytes following stimulation with ascorbate and cyanide are compared in a group of uraemic patients on chronic haemodialysis and in one treated with capd. lower baseline concentrations and smaller production of mda following in vitro stimulation, point to a better function of the pentose-phosphate shunt in capd patients than in haemodialysis.20133870550
thyroid stable iodine measurement in patients with hyperthyroidism. 20133832800
[aids: each and every one's responsibility].aids is posing challenges at various public health related levels, epidemiological, clinical, social, ethical, legal. in the current absence of possibilities of curative treatment or of vaccine, it is essential to provide sufficient information on the available preventive measures. given the increase in the numbers of seropositive persons, and the recognition that heterosexual relations area significant transmission route in case of multiple, illknown partners, the need is to actively promote th ...20133811612
the neuropathology of alzheimer's disease: a review with pathogenetic, aetiological and therapeutic considerations.the neuropathology of alzheimer's disease is reviewed in this paper emphasizing the morphological and morphometric changes that occur in the disease and their relationship to age and ageing. from this, a new hypothesis of pathogenesis is presented which accounts for the pattern of neuronal damage in alzheimer's disease. this is that the pathogenesis of alzheimer's disease begins with a leakage of a neurotoxin through a defective cortical blood brain barrier. this incites development of the senil ...20133906293
sales incentives for marketers. 20133679134
[spectral energy distributions of the /s/ sound in language development disordered children]. 20133710331
improving the donor site of the radial forearm flap.since its introduction (yang et al., 1981; song et al., 1982), the radial forearm flap has proved to be one of the safest and most versatile free flaps. however, the donor defect is significant and an otherwise excellent result is often impaired by a poor graft take over the exposed flexor carpi radialis tendon, even when great care has been taken to preserve its paratenon. this results in delayed healing, with frequent dressing changes, and often produces an area that is later prone to breakdow ...20134052711
burns of the upper extremity in children: long-term evaluation of function following treatment.hand burns in children represent a severe and possibly deforming injury. this study evaluated 82 patients for type of burn, prognostic indicators, and treatment as it affected function and appearance. the best long-term results were associated with small palmar contact burns, compression garments, early skin grafting except for the palm, and late surgical release of webbing. range of motion of the joints in the hand and clinical function were related. however, hand appearance had no correlation ...20133624468
[monoclonal antibodies--a new tool for research, diagnosis and therapy]. 20133553818
humoral immune function in severe, active rheumatoid arthritis.peripheral blood mononuclear cells (pbmc) from 30 patients with definite or classic active rheumatoid arthritis who were on no remittive drugs were studied for spontaneous and pokeweed mitogen (pwm)-stimulated immunoglobulin plaque-forming cell frequency (igpfc), spontaneous igm-rheumatoid factor (igm-rf) secretion, and in vitro proliferative responses to soluble recall antigens. rheumatoid spontaneous total (igg + igm + iga) igpfcs were higher than those of normal controls when assayed after 7 ...20133552341
[diagnostic contribution of computer tomography in muscular pathology].ct analysis in 26 patients with well-identified muscle diseases, and 4 normal controls was performed to evaluate its diagnostic interest. scans were obtained through pelvis, thigh and calves at comparable levels in all subjects. results showed that elementary changes were devoid of specificity and density measurements did not significantly increase the diagnostic significance of images. a good correlation was found between the degrees of ct changes and clinical evaluation of the muscles. however ...20133937199
esophageal atresia. corrective procedures and nursing care. 20133647749
[reaction of the eye to absence of the vitreous body]. 20133623377
[effects of long-term administration of piretanide on blood potassium]. 20133757466
[problems in using health standards in protection from an electrical field]. 20133744081
[fulminating gangrene of the external sex organs]. 20133811586
haematuria: a red herring? 20133697624
[care for the elderly in europe: denmark (part 1)]. 20133650546
pharmacokinetics of atracurium and its metabolites.the pharmacokinetic profile of atracurium was studied in normal patients and in patients with renal failure, renal-hepatic failure, or hepatic disease. its short elimination half-life was not significantly altered by renal failure, but in patients with severe liver disease elimination of its metabolites was prolonged, necessitating care during long-term i.v. infusions in patients with hepatic dysfunction.20133754756
[risks of postcoital contraception using a d-norgestrel-ethinyl estradiol combination]. 20133726780
[conduction anesthesia by cold block. a new possibility in the treatment of chronic pain]. 20133683328
the management of venous injuries in blunt extremity trauma.data on venous injury in blunt extremity trauma are scarce. the nature of injury and results of therapy in 22 patients presenting with blunt venous extremity injury at miemss and kumc in a 10-year period are presented. comparisons of the outcome with venous ligation and repair, the use of anticoagulants, and the use of fasciotomy, led to the development of guidelines for the therapy of blunt venous injury in both upper and lower extremity trauma.20133973943
the management of venous injuries in blunt extremity trauma.data on venous injury in blunt extremity trauma are scarce. the nature of injury and results of therapy in 22 patients presenting with blunt venous extremity injury at miemss and kumc in a 10-year period are presented. comparisons of the outcome with venous ligation and repair, the use of anticoagulants, and the use of fasciotomy, led to the development of guidelines for the therapy of blunt venous injury in both upper and lower extremity trauma.20133973943
the relation of various enzymes to cellular membranes. 20134289513
the relation of various enzymes to cellular membranes. 20134289513
spotlight on children. the silent victims. 20133853165
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