Publications

[the correspondence theory and etiological structure of shigella infections].		1992	1509850
[sensitivity of shigella to antibiotics].	three hundred and twenty two shigella cultures isolated from dysentery patients within 1986-1989 were tested with the use of standard paper disks for their sensitivity to levomycetin, streptomycin, tetracycline, monomycin, neomycin, kanamycin, erythromycin, gentamicin, carbenicillin, ampicillin, oxacillin, methicillin and doxycycline. the number of the cultures belonging to shigella sonnei amounted to 85.1 per cent of the total number of the strains studied. 91.9, 89.5, 87.3, 87.3, 80.1 and 80.1 ...	1992	1514848
single-dose ciprofloxacin for shigellosis in adults.		1992	1522320
titres of class-specific antibodies against shigella and salmonella lipopolysaccharide antigens in colostrum and breast milk of costa rican, swedish and vietnamese mothers.	enzyme immunoassays (eia) were used to estimate titres of class-specific antibodies against purified and chemically defined phenol-water-extracted lipopolysaccharide (lps) antigens of salmonella serogroup b (bo), shigella dysenteriae type i, plesiomonas shigelloides (the same o-antigen as shigella sonnei) and shigella flexneri y. titres in colostrum and breast milk of swedish, vietnamese and costa rican mothers from various socioeconomic conditions were compared. the antibodies were mainly of th ...	1992	1522329
efficacy of bovine milk immunoglobulin concentrate in preventing illness after shigella flexneri challenge.	the protective efficacy of oral bovine immunoglobulin concentrates derived from colostrum against challenge with shigella flexneri was studied in healthy adult volunteers in a randomized, double-blind fashion. volunteers were given a product consisting of hyperimmune immunoglobulin concentrate with a high titer of anti-s. flexneri 2a lipopolysaccharide (lps) with sodium bicarbonate or a control preparation with sodium bicarbonate three times a day for seven days. on the third day of treatment, v ...	1992	1524140
influence of inhibitors of lipid biosynthesis on the production of avermectins in streptomyces avermitilis.	streptomyces avermitilis was grown in the presence of four inhibitors of fatty acid biosynthesis (p-chlorophenoxyacetic, dl-2-(p-chlorophenoxy)-propionic, 2-(p-chlorophenoxy)-2-methyl-propionic and p-fluorophenoxyacetic acids). the production of total lipids was inhibited and the cellular content of individual types of fatty acids was altered by these compounds. the anteiso-acid content decreased by up to one-half, whereas the content of iso-odd acids increased 10-fold in the presence of inhibit ...	1992	1526463
low levels of coagulation inhibitors in patients with clostridium difficile infection.	to investigate levels of coagulation inhibitors in sera from patients with clostridium difficile-associated diarrhoea and colitis, commercially available antigen assays were used for immunochemical determination of antithrombin iii, protein c and free protein s. sera from patients with clostridium difficile-associated diarrhoea and colitis showed significantly lowered levels of all measured inhibitors as compared to controls (student's t test). protein c (mean +/- sd): 0.70 +/- 0.30 vs. 1.28 +/- ...	1992	1533851
risk factors for suicide attempts in a human immunodeficiency virus screening program.		1992	1539099
carcinoid tumor of the pancreas with obstructive pancreatitis.	a case of primary pancreatic enterochromaffin cell (ec cell) carcinoid tumor in a 66-yr-old japanese man is presented. the markedly dilated main pancreatic duct was noticed at the time of an incidental ultrasonogram during hospitalization for diabetes mellitus. endoscopic retrograde pancreatography showed stenosis of the main pancreatic duct at the body of the pancreas and dilated pancreatic ducts distal to the obstruction. a distal pancreatectomy was performed, together with splenectomy. macros ...	1992	1539573
relationship between intracellular ph and tension development in resting ventricular muscle and myocytes.	simultaneous measurements of intracellular ph (phi) and tension development were made in resting cat papillary muscles and resting ventricular myocytes (cat, guinea pig). ph microelectrodes and the fluorescent indicator carboxy-seminaphthorhodafluor-1 (snarf-1) were used to measure phi in muscles and myocytes, respectively. phi-induced changes in isometric muscle tension and myocyte length were elicited by variations in pco2, hco3-, and [nh4cl]. increased phi elevated resting tension and decreas ...	1992	1539624
fluid shear stress modulates cytosolic free calcium in vascular endothelial cells.	cytosolic free ca2+ concentration ([ca2+]i) was monitored in single and groups of fura-2-loaded bovine aortic endothelial cells (baec) during exposure to laminar fluid shear stress. application of a step increase in shear stress from 0.08 to 8 dyn/cm2 to confluent baec monolayers resulted in a transient increase in [ca2+]i, which attained a peak value in 15-40 s, followed by a decline to baseline within 40-80 s. the magnitude of the [ca2+]i responses increased with applied shear stress over the ...	1992	1539628
degradation of 2,4-dinitrotoluene by the lignin-degrading fungus phanerochaete chrysosporium.	under ligninolytic conditions, the white rot basidiomycete phanerochaete chrysosporium mineralizes 2,4-dinitrotoluene (i). the pathway for the degradation of i was elucidated by the characterization of fungal metabolites and oxidation products generated by lignin peroxidase (lip), manganese peroxidase (mnp), and crude intracellular cell extracts. the multistep pathway involves the initial reduction of i to yield 2-amino-4-nitrotoluene (ii). ii is oxidized by mnp to yield 4-nitro-1,2-benzoquinone ...	1992	1539977
salmonella peritonitis and splenic abscess in a patient with systemic lupus erythematosus.		1992	1540025
movement along actin filaments of the perijunctional area and de novo polymerization of cellular actin are required for shigella flexneri colonization of epithelial caco-2 cell monolayers.	shigella flexneri invades eucaryotic cells and grows in the cytoplasm. lysis of the phagosomal membrane is a prerequisite for both intracellular multiplication and movement of the bacteria that gain direct access to the host cell actin. in hela cells, bacteria generate their own movement essentially by inducing actin polymerization. polymerization of actin enables them to move rapidly and randomly in the cytoplasm and to spread from one cell to another through protrusions of the host cell membra ...	1992	1541518
nucleotide sequence and transcriptional regulation of a positive regulatory gene of shigella dysenteriae.	a 1,937 bp psti-hindiii fragment containing the ipar locus was cloned from the large invasion plasmid of shigella dysenteriae cg097, and its nucleotide sequence was completely determined. the ipar protein (35 kda, calculated from the dna sequence) was synthesized in escherichia coli chi 1411 minicells containing the 1,937-bp psti-hindiii fragment. to determine the regulatory role of ipar for ipa genes, we applied genetic complementation experiments using chloramphenicol acetyltransferase (cat) a ...	1992	1541532
shigella-specific iga in saliva of children with bacillary dysentery.	to study the secretory immune response after shigella infection, the anti-lipopolysaccharide and anti-shiga-toxin response in saliva, obtained from children with confirmed shigellosis and healthy children, were determined by enzyme-linked immunosorbent assay and by western blot. children with infection showed high titers compared to healthy controls. after shigella dysenteriae type 1 infection a significant change in titer could be observed in a large number of cases, in contrast to shigella fle ...	1992	1547024
drug resistance and plasmid profile of shigellae in taiwan.	one hundred and twenty-eight shigella strains isolated from newborn and infant human faecal specimens at kaohsiung medical college hospital in taiwan were serogrouped, serotyped and examined for drug-resistance patterns and for the presence of plasmids. forty-seven per cent of the isolates were found to belong to the shigella sonnei serogroup, 41% to the s. flexneri group, 9% to the s. boydii group and 3% to the s. dysenteriae group. the serotype with the greatest number of strains was s. sonnei ...	1992	1547844
clinical and biological evaluation in von willebrand's disease of a von willebrand factor concentrate with low factor viii activity.	this study was carried out to assess the clinical efficacy in von willebrand's disease (vwd) of a new, very high purity (vhp), solvent/detergent (sd)-treated, vwf concentrate (vhp human von willebrand factor concentrate, biotransfusion) characterized by a high specific ristocetin cofactor (vwf:rco) activity and a low factor viii (fviii) coagulant activity (fviii:c). nine patients (four type i, one type iia, one type iib, one type iic, one type iii and one acquired type ii) were infused on 13 occ ...	1992	1550779
[molecular and cellular bases of the virulence of shigella flexneri].	shigella flexneri, a gram negative bacillus, causes bacillary dysentery, an ulcerative disease of the human colon, by invading intestinal epithelial cells. entry into epithelial cells occurs via an induced phagocytic process which involves the actino-myosin complex. the host-cell receptor and the transmembrane signal which initiate reorganization of the cytoskeleton are under study. binding to integrins has recently been demonstrated in related models such as the entry of yersinia pseudotubercul ...	1992	1554236
auxotrophic live oral shigella flexneri vaccine protects monkeys against challenge with s. flexneri of different serotypes.	the aromatic-dependent live shigella flexneri y strain sfl114, attenuated by a tn10-inactivated arod gene, was given as an oral vaccine to 14 macaca fascicularis monkeys. a significant clinical attenuation of sfl114 was seen (p = 0.0058) as all vaccinated monkeys tolerated 2 x 10(10)-1 x 10(11) bacteria of sfl114, whereas four out of seven monkeys orally given 1 x 10(11) of the virulent parent strain sfl1 developed shigellosis. the average excretion time for sfl114 and sfl1 were 2 and 18 days, r ...	1992	1557932
endocrine responses in cows milked by hand and machine.	plasma concentrations of oxytocin, prolactin, and cortisol were compared in five swedish red and white cows milked by hand versus machine. cows were divided into two groups. one group was hand-milked; the other group was machine-milked. treatments were switched every other day. the experiment was carried out for 6 d. blood samples were taken prior to, during, and after milking and were assayed for hormones. more oxytocin and prolactin were released in hand-milked cows. there were no significant ...	1992	1560139
pulsatile growth hormone secretion during the breeding season in male reindeer and its association with hypophagia and weight loss.	male deer reduce food intake and lose weight during the breeding season (rut) in autumn. growth hormone (gh) is lipolytic in ruminants; therefore, pulsatile gh secretion was measured in three young reindeer (rangifer tarandus tarandus) bulls during the natural period of weight loss during rut. blood samples were withdrawn through a jugular cannula every 20 min for 16 hr on 5 occasions from late summer to early winter in unanaesthetised animals. the deer were group fed a pelleted concentrate rati ...	1992	1563616
shigellosis in thai children: epidemiologic, clinical and laboratory features.	two hundred thirty cases of shigellosis in children managed at chulalongkorn hospital from 1984 to 1988 were reviewed. the most common presentation included diarrhea, most frequently with mucus and blood, and fever. there were no deaths. the most common species was shigella flexneri (83.5%) which showed increasing resistance to trimethoprim-sulfamethoxazole.	1992	1565537
childhood shigellosis in saudi arabia.	in this study 234 children with shigellosis were evaluated during a 6-year period. the ages ranged from 2 days to 13 years (mean, 3.4 years). sixty percent of the children were in the 1- to 4-year age group. one hundred four children were hospitalized and 130 were outpatients. most cases of shigellosis presented during the months of april-may and september-november. shigella flexneri accounted for 44% and shigella sonnei for 43% of the isolates. susceptibility testing showed that 54% were resist ...	1992	1565538
lipoprotein lipase and hepatic lipase activities are differentially regulated in isolated hepatocytes from neonatal rats.	lipoprotein lipase and hepatic lipase are members of the lipase gene family sharing a high degree of homology in their amino acid sequences and genomic organization. we have recently shown that isolated hepatocytes from neonatal rats express both enzyme activities. we show here that both enzymes are, however, differentially regulated. our main findings are: (i) fasting induced an increase of the lipoprotein lipase activity but a decrease of the hepatic lipase activity in whole liver, being in bo ...	1992	1567912
bioactive polymers: in vitro and in vivo study of controlled release neomycin.	neomycin is coupled on xanthan-a polysaccharide of microbial biosynthesis produced by xanthomonas campestris-through ionic complexation. the kinetics of neomycin release, in vitro, at ph = 8.2 is studied. a controlled release of neomycin, following a zero order kinetics is observed, regardless of the eluent flow. neomycin complexed on xanthan, administered in a unique daily dose to patients suffering from dysentery in the 100 cases taken in study, has shown a high clinical efficiency as compared ...	1992	1573555
bacterial lipopolysaccharide in synovial fluid cells in shigella triggered reactive arthritis.		1992	1578474
ipab of shigella flexneri causes entry into epithelial cells and escape from the phagocytic vacuole.	by creating mutations within the shigella flexneri ipab gene, we have demonstrated that the invasion of epithelial cells is a three-step process encompassing adhesion on the cell surface, entry and lysis of the phagocytic vacuole allowing subsequent access to the cytoplasm. sc403, an insertion mutant which lacks expression of ipab but still expresses downstream genes, has been particularly studied. it is non-invasive, does not elicit actin polymerization, but binds to hela cells indicating that ...	1992	1582426
safety, immunogenicity, and efficacy in monkeys and humans of invasive escherichia coli k-12 hybrid vaccine candidates expressing shigella flexneri 2a somatic antigen.	a live, oral shigella vaccine, constructed by transfer of the 140-mda invasiveness plasmid from shigella flexneri 5 and the chromosomal genes encoding the group- and type-specific o antigen of s. flexneri 2a to escherichia coli k-12, was tested in humans. designated ecsf2a-1, this vaccine produced adverse reactions (fever, diarrhea, or dysentery) in 4 (31%) of 13 subjects who ingested a single dose of 1.0 x 10(9) cfu, while at better-tolerated doses (5.0 x 10(6) to 5.0 x 10(7) cfu), it provided ...	1992	1587589
novel invasion determinant of enteropathogenic escherichia coli plasmid plv501 encodes the ability to invade intestinal epithelial cells and hep-2 cells.	an escherichia coli k-12 transformant carrying 96.5-kb plasmid plv501 from enteropathogenic e. coli (epec) strain k798 is able to produce the same characteristic attaching-effacing lesions in a rabbit ileal biopsy explant model as its parent strain. cloned ecori-sali dna restriction fragments from this plasmid failed to reproduce the attaching-effacing lesions, but one recombinant plasmid, plv527, containing 4.5 kb of plv501 dna, conferred on e. coli dh1 transformants the ability to invade enter ...	1992	1587591
hla-b27 expression modulates gram-negative bacterial invasion into transfected l cells.	the mechanism by which hla-b27 confers genetic susceptibility to the seronegative spondyloarthropathies ankylosing spondylitis, reiter's syndrome, and reactive arthritis, is not well understood. the current concept of an extraarticular bacterial infection functioning as the triggering event in a genetically susceptible host suggests the possibility of direct microbial-mhc interaction. we have addressed the role of hla-b27 in microbial-host cell interaction by examining invasion by putatively art ...	1992	1588045
polymerase chain reaction analysis of renin in rat aortic smooth muscle.	controversy exists whether vascular smooth muscle cells in vivo synthesize renin, thereby providing a critical component of the hypothesized vascular renin-angiotensin system. to examine this question, we enzymatically isolated and pooled the medial layer of thoracic aortas from sprague-dawley rats that were either untreated or enalapril treated for 3 days, isolated messenger rna (mrna), and performed northern blot analysis with rat complementary dna (cdna) probes for renin, cathepsin d, and cat ...	1992	1592470
polymerase chain reaction analysis of renin in rat aortic smooth muscle.	controversy exists whether vascular smooth muscle cells in vivo synthesize renin, thereby providing a critical component of the hypothesized vascular renin-angiotensin system. to examine this question, we enzymatically isolated and pooled the medial layer of thoracic aortas from sprague-dawley rats that were either untreated or enalapril treated for 3 days, isolated messenger rna (mrna), and performed northern blot analysis with rat complementary dna (cdna) probes for renin, cathepsin d, and cat ...	1992	1592470
induction of beta-lactamase from shigella flexneri ucsf-129: purification by affinity chromatography and some properties.	lincomycin, an inhibitor of protein synthesis, was an excellent inductor of beta-lactamase, and its total activity and specific activity were increased 2.5- and 3.6-fold respectively. the beta-lactamase produced by shigella flexneri ucsf-129 was purified by affinity chromatography on phenylboronic acid-agarose with a type b column using an hydrophobic spacer arm (6-aminohexanoic acid activated with succinimide). the yield and specific activity were 96% and 29,283 u/mg, respectively. these were 1 ...	1992	1593966
acamprosate modulates synaptosomal gaba transmission in chronically alcoholised rats.	male sprague-dawley rats were pulmonary alcoholised for 30 days. six were treated with acamprosate (400 mg/kg/day, po) during alcoholisation. the control nonalcoholised group also received acamprosate (400 mg/kg/day, po) during the 30 days. at the end of the experiment, brains areas (cortex, hippocampus, thalamus, striatum, and olfactory bulbs) were dissected for the study of synaptosomal 3h-gaba uptake. in another experiment, gaba levels were determined in the same areas using hplc with electro ...	1992	1594633
copulatory analgesia in male rats ensues from arousal, motor activity, and genital stimulation: blockage by manipulation and restraint.	the effect of copulation on the vocalization threshold to tail shock (vtts) was assessed in freely-moving, sexually experienced, wistar male rats. mean vtts during the first copulation was 40% above the baseline values and slightly decreased during the first postejaculatory interval (pei; 28% above baseline). vtts mean values further increased during the second copulatory series (93% above baseline and 63% during the pei). testing at the same intervals in noncopulating rats did not induce analge ...	1992	1594675
metabolism of the aminoterminal propeptide of type iii procollagen in cultures of human proximal tubular cells.	degradation of the intact form of the aminoterminal propeptide of type iii procollagen (piiinp) has been established in the liver, whereas the col 1 domain of piiinp is extracted by the kidneys. we used native human piiinp and col 1 domain of piiinp to investigate the degradation of piiinp in cultures of human proximal tubular cells. normal renal tissue was obtained from the healthy part of kidneys surgically removed and from biopsies from a total of 10 patients. the degradation was characterize ...	1992	1594884
an isolated perfused rat kidney preparation designed for assessment of glomerular permeability characteristics.	the aim of the present investigation was to modify the widely used isolated perfused rat kidney preparation to make it more suitable for studies of glomerular permeability to macromolecules. both kidneys were perfused in situ using separate pumps in two of each other independent systems with tyrode-solution containing human serum albumin (18.2 g l-1). sodium nitroprusside was administered to induce dilatation and to maintain constant vascular resistance (pru100) during the experiments. the addit ...	1992	1595354
the no-reflow phenomenon is a post-mortem artifact.	post-ischemic reperfusion impairment, ("no-reflow phenomenon"), was studied in rats subjected to 8-30 minutes of global brain ischemia. during ischemia, rapid and complete loss of cerebral blood flow, eeg and 31p-high energy phosphates (atp/pcr) was observed. brain intravascular perfusion defects were examined by injecting carbon black intravenously in a group of rats with stable cardiopulmonary function and in another group subjected to rapid thoracotomy and intraarterial infusion of the carbon ...	1992	1595393
cross-reactive proteins of borrelia burgdorferi.	the specificity of serological tests for lyme borreliosis is impaired by cross-reacting antibodies. in order to select antigens for more specific tests, specific and cross-reactive proteins of borrelia burgdorferi must be identified. therefore, to analyze cross reactions of borrelia burgdorferi with other bacteria, rabbit immune sera against heterologous bacteria (borrelia hermsii, treponema pallidum, treponema phagedenis, leptospira interrogans (serogroup grippotyphosa), neisseria meningitidis, ...	1992	1597198
constitutive mutations of agrobacterium tumefaciens transcriptional activator virg.	the virulence (vir) genes of agrobacterium tumefaciens ti plasmids are positively regulated by virg in conjunction with vira and plant-derived inducing molecules. a procedure that utilizes both genetic selection and a genetic screen was developed to isolate mutations in virg that led to elevated levels of vir gene expression in the absence of vira and plant phenolic inducers. mutants were isolated at a frequency of 1 in 10(7) to 10(8). substitution mutations at two positions in the virg coding r ...	1992	1597431
live oral auxotrophic shigella flexneri sfl124 vaccine with a deleted arod gene: characterization and monkey protection studies.	shigella flexneri sfl124, with a deletion encompassing all, or nearly all, of the coding sequence of gene arod was obtained after selection on a fusaric acid medium supplemented with 2,3-dihydroxybenzoic acid for tetracycline-sensitive mutants of s. flexneri sfl114 which is an arod::tn10 transductant. two of 20 tetracycline-sensitive mutants tested in colony hybridization with a 32p-labelled dna probe of approximately 1400 base pairs (comprising all except the 75 n-terminal base pairs of the cod ...	1992	1598787
safety and immunogenicity of the live oral auxotrophic shigella flexneri sfl124 in volunteers.	the live, aromatic dependent shigella flexneri y vaccine strain sfl124, with a deleted arod gene, was tested for safety and immunogenicity in 21 healthy adult volunteers. a single dose of 2 x 10(9) live bacteria was given orally to ten volunteers, whereas 11 received three doses every other day. the vaccine was excreted for 4.2 days and was well tolerated by 90.5% of the vaccinees. only 2 of 21 (9.5%) after the first dose had a self-limiting diarrhoea lasting 1 day; of volunteers given one dose ...	1992	1598788
streptococcal toxic shock syndrome due to noninvasive pharyngitis.	serious infections due to group a beta-hemolytic streptococcus (gabhs) have been reported with increasing frequency in recent years. we report a case of toxic shock syndrome (tss) due to gabhs pharyngitis in an otherwise healthy 14-year-old boy. the organism was found to produce toxin a. to our knowledge, this is the second reported case of streptococcal tss associated with the production of toxin a that is not associated with an invasive disease and the first case associated with a documented r ...	1992	1600009
phosphorylation of icsa by camp-dependent protein kinase and its effect on intracellular spread of shigella flexneri.	shigella flexneri, a gram-negative bacillus belonging to the family enterobacteriaceae, causes bacillary dysentery in humans by invading colonic epithelial cells. processes by which epithelial cells, which are not professional phagocytes, may limit the spread of the invading microorganisms are poorly understood. this paper shows that icsa (virg), a 120 kda bacterial outer membrane protein responsible for intracellular and cell-to-cell spread through polymerization of actin, is a major substrate ...	1992	1602963
age-specific prevalence of serum antibodies to the invasion plasmid and lipopolysaccharide antigens of shigella species in chilean and north american populations.	shigella species have virulence plasmids that encode outer membrane proteins (invasion plasmid antigens, ipa) associated with pathogenicity. western blots were used to detect antibodies to ipa in sera from 390 chilean children, and these responses were compared with those of a us population of infants and adults. antibodies to lipopolysaccharide (lps) of plesiomonas shigelloides and shigella flexneri 2a were measured by elisa. among the chileans, there was an age-related acquisition of ipa antib ...	1992	1607690
adhesion of shigella dysenteriae type 1 and shigella flexneri to guinea-pig colonic epithelial cells in vitro.	adhesion of bacteria to guinea-pig colonic epithelial cells in vitro was inhibited by fucose with all the four strains tested (two of shigella dysenteriae type 1 and two of s. flexneri). n-acetyl neuraminic acid and n-acetyl mannosamine also caused inhibition, suggesting a multiplicity of receptors on the epithelial cell. congo-red binding of the strains correlated with their adhesive ability, whereas haemagglutination of rabbit erythrocytes by the bacteria did not.	1992	1613779
shigella flexneri induces apoptosis in infected macrophages.	the gram-negative bacterial pathogen shigella flexneri causes dysentery by invading the human colonic mucosa. bacteria are phagocytosed by enterocytes, escape from the phagosome into the cytoplasm and spread to adjacent cells. after crossing the epithelium, shigella reaches the lamina propria of intestinal villi, the first line of defence. this tissue is densely populated with phagocytes that are killed in great numbers, resulting in abscesses. the genes required for cell invasion and macrophage ...	1992	1614548
antimicrobial resistance in streptococcus pneumoniae: a south african perspective.	resistance to penicillin among south african strains of streptococcus pneumoniae increased from 4.9% in 1979 to 14.4% in 1990. except for resistance to co-trimoxazole (44%), resistance to other antimicrobial agents remained relatively low. multiply resistant strains belonged mainly to serovars 6b, 19a, 14, and, more recently, 23f. use of chloramphenicol to treat meningitis caused by strains relatively resistant to penicillin proved to be unsatisfactory, probably because of the inadequate bacteri ...	1992	1617077
virulence phenotypes of shigella flexneri 2a avirulent mutant 24570 can be complemented by the plasmid-coded positive regulator virf gene.	avirulent mutation of an opaque colony variant of shigella flexneri 2a designated 24570 has been believed to be linked with the glpk locus of the chromosome. however, avirulent phenotypes of the 24570 strain could be complemented by the invasion plasmid-coded virf gene, a positive regulator for invasion genes. the 24570 strain had a dna structural alteration upstream of the virf gene.	1992	1624118
molecular and functional characterization of the salmonella invasion gene inva: homology of inva to members of a new protein family.	one of the earliest steps in the pathogenic cycle of the facultative intracellular pathogen salmonella spp. is the invasion of the cells of the intestinal epithelium. we have previously identified a genetic locus, inv, that allows salmonella spp. to enter cultured epithelial cells. inva is a member of this locus, and it is the first gene of an operon consisting of at least two additional invasion genes. we have constructed strains carrying nonpolar mutations in inva and examined the individual c ...	1992	1624429
[shigella flexneri strains having a new type antigen].	five shigella strains isolated from stool cultures of two domestic dysentery cases and three imported cases in tokyo in 1986-1989 had an atypial serotype that agglutinated with only antiserum to s. flexneri group 6 factor antigen. these strains showed typical biochemical reactions of s. flexneri and were biochemically identical. four of the strains were positive for serény test with guinea pig eye and the cell-invasion test with hela cells. the strains also had the virulence-plasmid encoding out ...	1992	1624844
aida-i, the adhesin involved in diffuse adherence of the diarrhoeagenic escherichia coli strain 2787 (o126:h27), is synthesized via a precursor molecule.	the adherence mechanisms of enteropathogenic escherichia coli (epec) to epithelial cells are still not understood. to study the molecular basis of the diffuse adherence (da) phenotype exhibited by diarrhoeagenic e. coli expressing classical epec serotypes we investigated strain 2787 (o126:h27) isolated from a case of infantile diarrhoea. a 6.0 kb plasmid-derived dna fragment mediates the da phenotype and encodes the 100 kda adhesin protein aida-i (adhesin involved in diffuse adherence). sequenci ...	1992	1625582
the virf protein from shigella flexneri is a member of the arac transcription factor superfamily and is highly homologous to rns, a positive regulator of virulence genes in enterotoxigenic escherichia coli.		1992	1625585
[current insights on the pathogenesis of chronic inflammatory bowel diseases].	the pathogenesis and etiology of inflammatory bowel disease (ibd) is poorly understood. as a matter of fact, it is not even certain whether either one is a single entity with different forms of clinical manifestations, or whether each one represents a single clearly separable entity. common features of both diseases are chronic persistence, recurrent exacerbation and remission, the production of autoantibodies, as well as the expression of aberrant hla-class ii molecules on the surface of epithe ...	1992	1626162
[photodynamically induced vitreous liquefaction in vivo].	photodynamically induced vitreous liquefaction in rabbit eye was investigated. photosensitizer, riboflavine phosphate, was injected into the vitreous cavity of the rabbit before white-light irradiation. after the irradiation (0, 1, 3, 6 hr) the rabbit vitreous body was separated into gel and liquid portions. the liquid vitreous body was weighed, and the vitreous liquefaction percentage was calculated. one hour irradiation caused 38% of liquefaction of the eye; 3 hr, 50% liquefaction; 6 hr, 59% l ...	1992	1626474
antibodies to shiga holotoxin and to two synthetic peptides of the b subunit in sera of patients with shigella dysenteriae 1 dysentery.	acute- and convalescent-phase sera from 18 thai patients and convalescent-phase sera from two israeli patients and one bangladeshi patient with shigella dysenteriae 1 (shiga) dysentery were tested by enzyme-linked immunosorbent assay to detect antibodies that bind s. dysenteriae lipopolysaccharide (lps), shiga holotoxin, or two synthetic peptides representing epitopes from the b subunit of shiga toxin. paired sera from 24 maryland adults with shigella flexneri 2a or shigella sonnei diarrhea serv ...	1992	1629317
[an interesting case--endoscopic hemostasis of an actively bleeding sigmoid diverticulum--a case report].	a pulsating arterial bleeding from a diverticulum, situated in the colon sigmoideum, was identified and stopped during video-documentation in a 70 year-old man. after injection with a solution of suprarenin, the diverticulum was turned out like a balloon into the colon and didn't show any signs of further bleeding until the patient was prepared for a semi-elective resection of the sigmoid. this case demonstrates that emergency endoscopy is an effective method for diagnosis and even therapy of ma ...	1992	1636274
mxia of shigella flexneri 2a, which facilitates export of invasion plasmid antigens, encodes a homolog of the low-calcium-response protein, lcrd, of yersinia pestis.	the plasmid-encoded invasion plasmid antigen (ipa) export accessory locus of shigella flexneri 2a, mxia, was cloned, and the complete dna sequence of the gene was determined. the mixa open reading frame was found to encode a polypeptide of 74.03 kda with a pi of 5.02. a hydropathy analysis of the predicted protein revealed a hydrophilic c terminus and an extremely hydrophobic n terminus without a cleavable signal sequence but with several potential membrane-spanning regions. while a homology sea ...	1992	1639496
beneficial effect of syngeneic pancreatic islet transplantation on liver atrophy and hepatocellular function after portacaval shunt.	hepatic insufficiency, which continues to be a source of morbidity after portacaval shunt (pcs), can be prevented by syngeneic pancreatic islet transplantation into the portal vein before pcs. this study investigated the ability of syngeneic pancreatic islet transplantation after pcs to prevent hepatic atrophy and rescue hepatocellular function.	1992	1641769
monoclonal antibodies against salmonella porins: generation and characterization.	monoclonal antibodies (mabs) were generated against porins, one of the major outer membrane proteins of salmonella typhi. six clones, designated mp1, mp2, mp3 (igg2ak), mpn4, mpn6 (igg1k) and mpn5 (igg2bk) were characterized by enzyme immunoassay (elisa) for their reactivity to porins from s. typhi, salmonella paratyphi a, s. paratyphi b, s. paratyphi c, salmonella choleraesuis, salmonella enteritidis, salmonella krefeld, salmonella panama, salmonella typhimurium, escherichia coli b, shigella fl ...	1992	1280248
acetylation of o-specific lipopolysaccharides from shigella flexneri 3a and 2a occurs in escherichia coli k-12 carrying cloned s. flexneri 3a and 2a rfb genes.	most of the shigella flexneri o-specific serotypes result from o-acetyl and/or glucosyl groups added to a common o-repeating unit of the lipopolysaccharide (lps) molecule. the genes involved in acetylation and/or glucosylation of s. flexneri lps are physically located on lysogenic bacteriophages, whereas the rfb cluster contains the biosynthesis genes for the common o-repeating unit (d.a.r. simmons and e. romanowska, j. med. microbiol. 23:289-302, 1987). using a cosmid cloning strategy, we have ...	1992	1280255
immunocytochemistry of oculomotor afferents in the squirrel monkey (saimiri sciureus).	attempts were made to co-define afferents of the oculomotor nuclear complex (omc) and their putative neurotransmitters in the squirrel monkey. wheat germ agglutinin conjugated to horseradish peroxidase (wga-hrp) and wheat germ agglutinin conjugated to enzymatically inactive hrp and coupled to colloidal gold (wgaapohrp-au) were used as retrograde tracers in combination with immunocytochemical methods. primarily unilateral injections were made into portions of the omc. stabilized tetramethylbenzid ...	1992	1280294
development of a competitive radioimmunoassay and a sandwich enzyme-linked immunosorbent assay for recombinant human granulocyte colony-stimulating factor. application to a pharmacokinetic study in rats.	a competitive radioimmunoassay (ria) and a sandwich enzyme-linked immunosorbent assay (elisa) for the measurement of recombinant human granulocyte colony-stimulating factor (rhg-csf) in rat serum was developed using polyclonal antibody obtained by immunizing rabbits with rhg-csf. the anti-rhg-csf antibody did not recognize other colony-stimulating factors or interleukins. ria and elisa gave satisfactory reproducibility as judged by intra- and inter-assay precision. good agreements between the ri ...	1992	1280299
differentiation of shigella flexneri strains by rrna gene restriction patterns.	we studied the restriction endonuclease cleavage patterns of rrna genes (ribotypes) of 72 clinical isolates of shigella flexneri representing eight serotypes to determine whether ribotyping could be used to distinguish s. flexneri strains and to compare the discriminating ability of the method with that of serotyping. by using a cloned escherichia coli rrna operon as the probe, southern blot hybridization of restriction endonuclease-digested total dna was carried out. ribotyping of the isolates ...	1992	1280647
regeneration of an adult peripheral nerve preparation in culture.	the methods used to maintain the vagus nerve from the adult rat in culture and how regeneration is studied in this preparation are described. a hypothesis is presented on the triggering of the cell body reaction. it is suggested that this reaction is initiated by proteins synthesized in nonneuronal cells at the site of a nerve lesion. these proteins, referred to as regenerins, reach the nerve cell body by retrograde axonal transport, where they initiate the regeneration process.	1992	1282333
computer-assisted analysis of the structure of regular branched polysaccharides containing 2,3-disubstituted rhamnopyranose and mannopyranose residues on the basis of 13c nmr data.	a computer-assisted approach to the analysis of the structure of branched polysaccharides that contain 2,3-di-o-glycosylated alpha-rhamnopyranose and alpha-mannopyranose residues is based on evaluation of the 13c nmr spectra, using glycosylation effects and their deviations from additivity (delta delta values) at the branch points. this approach, in combination with monosaccharide and methylation analysis data, has been verified on a series of bacterial polysaccharides of known structure.	1992	1284111
multiresistant shigella species from african aids patients: antibacterial resistance patterns and application of the e-test for determination of minimum inhibitory concentration.	the antibacterial resistance pattern and minimum inhibitory concentrations (mic) of 25 shigella flexneri, 5 s. boydii, 8 s. sonnei, and 3 strains of s. dysenteriae type 2 isolated from kenyan prostitutes with bacillary dysentery and aids were determined, and the applicability of the e-test for mic determination evaluated. all strains were resistant to > or = 3 of 9 different antibacterial agents tested. all strains were resistant to tetracycline and erythromycin, 95% to trimethoprim/sulfonamide, ...	1992	1287807
orientation bandwidth: the effect of spatial and temporal frequency.	the orientation bandwidths of psychophysically defined channels of human vision were estimated by two techniques for a wide range of spatial and temporal frequencies. the first technique was an adaptation paradigm, where the subjects' ability to see patterns of various orientations was measured before and after adapting to a high contrast pattern. the second technique evaluated subjects' ability to discriminate between two gratings of different orientations in relation to their ability to detect ...	1992	1287993
[the intoxication syndrome in acute dysentery].		1992	1293830
[shigella flexneri strains having a new type antigen 89-141].	two shigella strains (89-141 and 89-11) isolated from the stool of patients returning from abroad (both from india) in tokyo in 1989 showed an atypical serologic reaction of agglutination with only polyvalent antiserum to s. flexneri prepared commercially. these strains had the typical biochemical characteristics of s. flexneri and were biochemically identical. both strains were positive for serény test in guinea pig eye and cell-invasion test in hela cells. the strains also had virulence-plasmi ...	1992	1294667
[studies of the lymphatic system in mice infected with trichinella pseudospiralis as well as infected and stimulated by phytohaemagglutin (pha-p)].	mice cfw were infected per os with trichinella pseudospiralis (150 larvae/mice). the mice were divided in two groups: nonstimulated and stimulated intraperitoneally with pha-p at dose 10 mg/kg body weight. the animals were killed 7, 14, 21, 28, 42, 56, 70 and 150 day post infection (d.p.i.). spleen et mesenteric lymph nodes were examined histopathologically (hematoxylin-eosin) and histochemically (nonspecific esterase reaction) for differentiation of t lymphocytes. the obtained results reveal th ...	1992	1299055
effect of outer membrane proteins from shigella on humoral immunity induced in mice by srbc.	shigella flexneri outer membrane proteins (omp) which had been earlier found to exert immunomodulatory effect on cell mediated immune response were also found to act as immunomodulator of the humoral immune response. effects of omp were investigated in the experiments in vitro and in vivo, where the level of humoral immune response, measured as the number of plaque-forming cells (pfc) to srbc in the spleen was evaluated. we demonstrate that small doses of omp (1-5 micrograms) stimulate, whereas ...	1992	1299171
fraction of spleen cells responsible for suppression of cellular immune response to srbc in mice treated with outer membrane proteins of shigella.	effect of splenocytes isolated from mice immunized with suppressive dose of omp from shigella on delayed hypersensitivity, induced in mice with sheep red blood cells was investigated. only the population of t lymphocytes was found to suppress the delayed hypersensitivity, as measured by the footpad reaction. the results suggest that omp of shigella are able to induce in the spleens of animals active t cells which are responsible for the suppression of cellular response induced by srbc.	1992	1299172
[the clinical assessment of macrophage functional activity in patients with protracted dysentery and its correction with lysozyme and vitamin e].	prospects for the correction of disturbances in the macrophagal system with a combination of lysozyme and vitamin e in patients with a protracted course of dysentery caused by shigella flexneri 1b were studied. the phagocytic activity of macrophages (pam) was found to be suppressed as early as at the beginning of the disease. out of 38 persons repeatedly found to release shigellae 24 were administered polychemotherapy. pam indices in patients treated with lysozyme and tocopherol acetate were lik ...	1992	1301666
expression and release of neuroregulators during development: monoamines and neuropeptides.	the turnover of catecholamines (ca) was determined in the adrenal medulla and brain of rat fetuses and pups. in general we found a considerable increase soon after birth. the expression of mrna for ca-synthesizing enzymes was also considerably enhanced in the adrenals shortly after birth. furthermore, we demonstrated increased expression of neuropeptides after birth, increased synthesis of mrna encoding for neuropeptide y in the adrenals 24 h after birth; and considerable activation of the subst ...	1992	1306802
hypoxic-ischemic damage in the neonatal brain: excitatory amino acids.	perinatal brain damage is a major clinical problem. recent studies suggest that excitatory amino acids (eaas) may be important for the development of hypoxic-ischemic brain injury in the newborn. experimental work demonstrates that the immature brain is hypersensitive to the toxic effects eaa ('excitotoxicity'), hypoxic-ischemia is accompanied by an extracellular overflow of eaas and hypoxic-ischemic brain damage is reduced by eaa receptor antagonists. clinical investigations demonstrate the pre ...	1992	1306803
[tubo-ovarian transplantation in the treatment of sterility. experimental research].	microsurgical transposition of fallopian tube and ovary has the potential of being an efficient therapeutic treatment in patients with tubal sterility. the authors present their experience of microsurgical adnexal transplantation in rabbit by two different techniques: the first procedure by microvascular anastomosis of the ovarian vessels, the second one without vascular pedicle. function is evaluated at various time after grafting by: exploratory laparotomy on day 30 to establish whether circul ...	1992	1307024
[the effect of short-term adaptation to high-altitude conditions on human blood serum lipoproteins].		1992	1307051
spontaneous insertion of an is1-like element into the virf gene is responsible for avirulence in opaque colonial variants of shigella flexneri 2a.	colonial variation of shigella flexneri serotype 2a from the translucent (2457t) to the opaque form (2457o) occurs spontaneously once in 10(4) cell divisions, with concomitant loss of ipa gene expression and virulence. the appearance of 2457o was associated with the insertional inactivation of virf, an invasion plasmid-encoded positive regulator of ipa gene expression. plasmid pwr110, a tn5-tagged invasion plasmid that restores the invasive phenotype to plasmid-cured shigella derivatives, was co ...	1992	1309511
regulation of interleukin-1 receptors on att-20 mouse pituitary tumour cells.	to study the cellular mechanisms of interleukin-1 (il-1) in the pituitary corticotroph, we studied the properties of il-1 receptors on a mouse pituitary acth-producing cell line, att-20. scatchard plot analysis revealed a single type of receptor with a kd (dissociation constant) of 93 pm, and 482 binding sites/cell. [125i]il-1 alpha binding competed with il-1 alpha and il-1 beta in an equimolar fashion. a 24 h pre-incubation with either crh, epinephrine or nor-epinephrine increased the [125i]il- ...	1992	1312014
regulation of interleukin-1 receptors on att-20 mouse pituitary tumour cells.	to study the cellular mechanisms of interleukin-1 (il-1) in the pituitary corticotroph, we studied the properties of il-1 receptors on a mouse pituitary acth-producing cell line, att-20. scatchard plot analysis revealed a single type of receptor with a kd (dissociation constant) of 93 pm, and 482 binding sites/cell. [125i]il-1 alpha binding competed with il-1 alpha and il-1 beta in an equimolar fashion. a 24 h pre-incubation with either crh, epinephrine or nor-epinephrine increased the [125i]il- ...	1992	1312014
surface presentation of shigella flexneri invasion plasmid antigens requires the products of the spa locus.	an avirulent, invasion plasmid insertion mutant of shigella flexneri 5 (phs1059) was restored to the virulence phenotype by transformation with a partial hindiii library of the wild-type invasion plasmid constructed in pbr322. western immunoblot analysis of phs1059 whole-cell lysates revealed that the synthesis of the invasion plasmid antigens virg, ipaa, ipab, ipac, and ipad was similar to that seen in the corresponding isogenic s. flexneri 5 virulent strain, m90t. ipab and ipac, however, were ...	1992	1312536
correlation between coxsackie b1 virus replication and enhanced invasiveness of shigella flexneri.	coxsackie b1 virus infection enhances the susceptibility of cultured hep-2 cells to shigella flexneri invasiveness. this can be reproduced partially with uv-inactivated virus, particularly the effect observed shortly after viral inoculation. the following phases of viral multiplication were correlated with the enhancing effect: uncoating of viral particles, synthesis of viral rna and proteins, and assembly of newly produced virus particles. uncoating of virus particles was completed within 60 mi ...	1992	1314061
detection of porin antigen in serum for early diagnosis of mouse infections with salmonella typhimurium.	the monoclonal antibodies to porin, an outer membrane protein isolated from salmonella typhimurium and sandwich enzyme linked immunosorbent assay (elisa) has made possible the detection of porin from sera of s. typhimurium-infected mice. the specificity of the monoclonal antibodies was ascertained based on their cross-reactivity with porins isolated from s. typhi, shigella flexneri and escherichia coli and lipopolysaccharide (lps) of s. typhimurium and e. coli. serum samples were found to be pos ...	1992	1315558
use of adeno-associated virus as a general transduction vector for mammalian cells.		1992	1316261
global regulation of mitochondrial biogenesis in saccharomyces cerevisiae: abf1 and cpf1 play opposite roles in regulating expression of the qcr8 gene, which encodes subunit viii of the mitochondrial ubiquinol-cytochrome c oxidoreductase.	the multifunctional dna-binding proteins abf1 and cpf1 bind in a mutually exclusive manner to the promoter region of the qcr8 gene, which encodes 11-kda subunit viii of the saccharomyces cerevisiae mitochondrial ubiquinol-cytochrome c oxidoreductase (qcr). we investigated the roles that the two factors play in transcriptional regulation of this gene. to this end, the overlapping binding sites for abf1 and cpf1 were mutated and placed in the chromosomal context of the qcr8 promoter. the effects o ...	1992	1317009
kappa-opioid receptor stimulation abolishes mu- but not delta-mediated inhibitory control of spinal met-enkephalin release.	the possible opioid control through delta, mu and kappa receptors of the spinal release of met-enkephalin-like material (melm) was investigated in halothane-anaesthetized rats. the intrathecal perfusion of the delta agonist dtlet (10 microm) or the mu agonist dago (10 microm) resulted in a marked inhibition of melm release, which could be prevented by the selective antagonists naltrindole and naloxone, respectively. although the kappa agonist u 50488 h (10 microm) was inactive per se, it complet ...	1992	1317026
radiation-induced generation of chlorine derivatives in n2o-saturated phosphate buffered saline: toxic effects on escherichia coli cells.	the radiolysis of aqueous chloride solutions has been investigated using pulse and steady-state methods. we have found a correlation between the yields of cl2- and hocl formed in pulse-irradiated n2o-saturated solutions. the yields increased with the increasing concentrations of cl- and phosphate. phosphate enhanced the yield of cl2- in neutral solutions because of a proton transfer from h2po4- to hocl- with a rate constant of (2.6 +/- 0.5) x 10(8) m-1s-1. hocl could not be detected in pulse-irr ...	1992	1317323
ion channels activated by swelling of madin darby canine kidney (mdck) cells.	according to previous studies hyposmotic swelling of madin darby canine kidney (mdck) cells leads to a marked decrease of cell membrane resistance. the present study has been performed to identify the underlying ion channels using the patch-clamp technique: reduction of extracellular osmolarity to 230 mmol/liter leads to a transient activation of k+ channels and a sustained activation of anion channels. the k+ channels are inwardly rectifying with a single-channel slope conductance of 56 +/- 3 p ...	1992	1317457
plasma testosterone concentration in adult male rats following acquisition of copulation-illness associations.	prior research has identified stimuli and procedures that elicit increments in plasma testosterone in copulating male rats. in the present experiment, we demonstrate that associative inhibition of copulatory behaviors in male rats is not correlated with and cannot be attributed to a conditioned suppression of testosterone. each male rat was paired with an inaccessible estrous female for 7 min and was then given an opportunity to copulate. two groups received an injection of either lithium chlori ...	1992	1317591
coxsackie b1 virus-induced changes in cell membrane-associated functions are not responsible for altered sensitivity to bacterial invasiveness.	to analyze the possible mechanisms by which coxsackie b1 virus infection affects the invasiveness of shigella flexneri, we have studied the influence of intracellular levels of na+ and k+, atpase activity, cytoplasmic membrane potential, camp level and cell communication through gap junctions. 3h after adsorption of viable or uv-inactivated coxsackie b1 virus the na(+)-k+ gradient of the cell collapsed, atpase activity decreased, the cytoplasmic membranic potential-dependent tetraphosphonium ion ...	1992	1318709
new monoclonal antibodies for the detection of immediate early antigens of cytomegalovirus.	two new monoclonal antibodies, cie-1 and cie-2, were developed for the rapid detection of human cytomegalovirus (hcmv) infection. they were found to be reactive with immediate early protein of hcmv in the nuclei of infected fibroblasts, as early as 3 hours post-infection. by radioimmunoprecipitation, cie-1 was found to react with a protein with an apparent molecular weight of 70,000, whereas cie-2 precipitated 2 proteins of 70,000 and 72,000 daltons, respectively. both monoclonal antibodies reco ...	1992	1319172
lactoferrin-binding proteins in shigella flexneri.	the ability of shigella flexneri to interact with lactoferrin (lf) was examined with a 125i-labeled protein-binding assay. the percent binding of human lactoferrin (hlf) and bovine lactoferrin (blf) to 45 s. flexneri strains was 19 +/- 3 and 21 +/- 3 (mean +/- standard error of the mean), respectively. 125i-labeled hlf and blf binding to strain m90t reached an equilibrium within 2 h. unlabeled hlf and blf displaced the 125i-hlf-bacteria interaction in a dose-dependent manner. the lf-bacterium co ...	1992	1319403
enrofloxacin treatment of long-tailed macaques with acute bacillary dysentery due to multiresistant shigella flexneri iv.	thirty-four cases of acute bacillary dysentery occurred within 90 days among macaques housed at the california regional primate research center. cases were identified by depression, diarrhea with blood and leukocytic exudate, and/or leukocytosis with a left shift. antimicrobial susceptibility testing of enteric isolates and plasmid profile analyses established an etiologic diagnosis of multiple antibiotic resistant shigella flexneri iv infection. when standard therapies were invalidated by high ...	1992	1320152
serotonergic fibres degenerating in the aging rat brain or sprouting from grafted fetal neurons are not affected by the neurotrophic acth analogue org 2766.	the effects of chronic treatment with the purported neurotrophic factor acth(4-9) analogue org 2766 were studied on age-related degeneration of serotonergic fibres and on gliosis in the rat hippocampus and caudate putamen complex. in addition, the potential growth-promoting effects of org 2766 were investigated on fetal serotonergic cells implanted in a previously denervated hippocampus of young adult rats. chronic treatment of rats from the age of 11 months to 17-18 months did not affect the in ...	1992	1326283
[inhibitory effect of refined amorphophallus konjac on mnng-induced lung cancers in mice].	550 seven-wk-old laca mice were used in 3 batches for studying the inhibitory effect of refined amorphophallus konjac (konjaku powder) on mnng-induced lung cancers. the mice (within each batch) were randomly allocated into four groups, namely, positive control (mnng), amorphophallus konjac (a. k.), complex (mnng+a. k.), and blank control (c) groups. in mnng group, mnng (250 micrograms) was injected intravenously once every five days for seven times in each mouse, the total dosage of mnng being 1 ...	1992	1327690
interferon treatment reduced adherence, invasiveness and intracellular multiplication of shigella flexneri in coxsackie b1 virus-infected cells.	the effect of interferon treatment on interaction of shigella flexneri with in vitro cultured cells was investigated. pretreatment of hep-2 cells with human interferons had no effect on the susceptibility of cells to s. flexneri, measured by invasiveness and adhesiveness. human leukocyte interferon and human recombinant interferon-alpha-a reduced adhesiveness, intracellular multiplication and invasiveness of s. flexneri in hep-2 cells preinfected with coxsackie b1 virus. also non-receptor mediat ...	1992	1329437
regulation of virulence-associated plasmid genes in enteroinvasive escherichia coli.	the transposon tnphoa was used for construction of gene fusions and for studies of gene regulation in an enteroinvasive strain of escherichia coli. several plasmid-encoded virulence genes (e.g., the ipab and virg operons) of such enteroinvasive strains are subject to coordinated thermoregulation involving both operon-specific (the virb and virf activators) and global regulators. the nucleoid-associated e. coli protein h-ns was shown to be a negative regulator as judged by studies using h-ns gene ...	1992	1332938
mxij, a lipoprotein involved in secretion of shigella ipa invasins, is homologous to yscj, a secretion factor of the yersinia yop proteins.	shigella flexneri causes bacillary dysentery by invading epithelial cells of the colonic mucosa. the invasion process requires the synthesis and secretion of the virulence plasmid-encoded ipa proteins. using tnphoa mutagenesis, we have identified two virulence plasmid genes, mxij and mxim, that encode proteins exported by the general export pathway. analysis of the mxij and mxim deduced amino acid sequences suggested that mxij and mxim might encode lipoproteins, which was confirmed by [3h]palmit ...	1992	1332940