Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| global cooling: cold acclimation and the expression of soluble proteins in carp skeletal muscle. | the common carp (cyprinus carpio) has a well-developed capacity to modify muscle properties in response to changes in temperature. understanding the mechanisms underpinning this phenotypic response at the protein level may provide fundamental insights into the molecular basis of adaptive processes in skeletal muscle. in this study, common carp were subjected to a cooling regimen and soluble extracts of muscle homogenates were separated by 1-d sds-page and 2-de. proteins were identified using mal ... | 2007 | 17623276 |
| acute toxicity of antimony chloride and its effects on oxygen consumption of common carp (cyprinus carpio). | the purposes of this study were to investigate the acute toxicity and effects of sublethal antimony (sb) concentrations on respiratory activity changes in the common carp (cyprinus carpio). median lethal concentrations were determined in acute tests. the 96-h lc50 value was 14.05 (11.09~17.80) mg l(-1). common carp were exposed to 4 different sublethal levels of antimony (1.0, 2.0, 4.0, and 8.0 mg l(-1)) over a 28-day test period and a 14-day recovery period. on days 14 and 28, decreases in oxyg ... | 2007 | 17618378 |
| a comparative study on innate immune parameters in the epidermal mucus of various fish species. | fish epidermal mucus and its components provide the first line of defense against pathogens. little is known about the role of epidermal mucus enzymes in the innate immune system of fish species such as arctic char (salvelinus alpinus), brook trout (s. fontinalis), koi carp(cyprinus carpio), striped bass (morone saxatilis), haddock, (melanogrammus aeglefinus), atlantic cod (gadus morhua) and hagfish (myxine glutinosa). the epidermal mucus samples from these fish were analysed for the specific ac ... | 2007 | 17618153 |
| the nuclear phenotypic plasticity observed in fish during rrna regulation entails cajal bodies dynamics. | cajal bodies (cbs) are small mobile organelles found throughout the nucleoplasm of animal and plant cells. the dynamics of these organelles involves interactions with the nucleolus. the later has been found to play a substantial role in the compensatory response that evolved in eurythermal fish to adapt to the cyclic seasonal habitat changes, i.e., temperature and photoperiod. contrary to being constitutive, rrna synthesis is dramatically regulated between summer and winter, thus affecting ribos ... | 2007 | 17588531 |
| the complete mitochondrial genome of the chinese hook snout carp opsariichthys bidens (actinopterygii: cypriniformes) and an alternative pattern of mitogenomic evolution in vertebrate. | the complete mitochondrial genome sequence of the chinese hook snout carp, opsariichthys bidens, was newly determined using the long and accurate polymerase chain reaction method. the 16,611-nucleotide mitogenome contains 13 protein-coding genes, two rrna genes (12s, 16s), 22 trna genes, and a noncoding control region. we use these data and homologous sequence data from multiple other ostariophysan fishes in a phylogenetic evaluation to test hypothesis pertaining to codon usage pattern of o. bid ... | 2007 | 17587513 |
| carp (cyprinus carpio) vitellogenin: characterization of yolk proteins, development of immunoassays and use as biomarker of exposure to environmental estrogens. | the precursor protein of egg yolk, vitellogenin (vg), is cleaved into three major components (lipovitellin, phosvitin and beta'-component) at the time of incorporation by growing oocytes. we purified three yolk proteins (yp1, yp2 and yp3) from ovaries of the common carp (cyprinus carpio) by a combined method of ammonium sulfate precipitation and column chromatography. biochemical analyses of the purified proteins of this species suggest that yp1, yp2 and yp3 are lipovitellin, beta'-component and ... | 2007 | 17585296 |
| carp versus cranium: a fishy story. | issues of drowning or near drowning in aquatic sporting have received considerable attention in the literature; however, there is not a lot of attention to nonsubmersion injuries such as water tubing. water tubing is similar to water skiing but with much less control by the rider, leaving the rider at the mercy of the driver or any obstacle in their path. this report describes unusual events surrounding the injury of a 5-year-old boy enjoying water fun with his father on his boat. the importance ... | 2007 | 17579328 |
| functional characterisation of ucp1 in the common carp: uncoupling activity in liver mitochondria and cold-induced expression in the brain. | mammalian uncoupling protein 1 (ucp1) mediates nonshivering thermogenesis in brown adipose tissue. we previously reported on the presence of a ucp1 orthologue in ectothermic fish and observed downregulation of ucp1 gene expression in the liver of the common carp. neither the function of ucp1, nor the mode of ucp1 activation is known in carp liver mitochondria. here, we compared the proton conductance at 25 degrees c of liver mitochondria isolated from carp either maintained at 20 degrees c (warm ... | 2007 | 17576568 |
| sequential ultrastructural and biochemical changes induced in vivo by the hepatotoxic microcystins in liver of the phytoplanktivorous silver carp hypophthalmichthys molitrix. | up to now, in vivo studies on the toxic effects of microcystins (mcs) on the ultrastructures of fish liver have been very limited. the phytoplanktivorous silver carp was injected i.p. with extracted hepatotoxic microcystins (mainly mc-rr and -lr) at a dose of 1000 microg mc-lreq. kg(-1) body weight, showing a time-dependent ultrastructural change in liver as well as significant increases in enzyme activity of plasma alanine aminotransferase (alt), aspartate aminotransferase (ast) and lactate deh ... | 2007 | 17574927 |
| kinematics, hydrodynamics and energetic advantages of burst-and-coast swimming of koi carps (cyprinus carpio koi). | koi carps frequently swim in burst-and-coast style, which consists of a burst phase and a coast phase. we quantify the swimming kinematics and the flow patterns generated by the carps in burst-and-coast swimming. in the burst phase, the carps burst in two modes: in the first, the tail beats for at least one cycle (multiple tail-beat mode); in the second, the tail beats for only a half-cycle (half tail-beat mode). the carp generates a vortex ring in each half-cycle beat. the vortex rings generate ... | 2007 | 17562892 |
| [3h] muscimol receptors sites in the carp (cyprinus carpio l.) brain: binding assay and autoradiographic distribution. | autoradiographic and binding techniques were used to study the presence of [(3)h]muscimol receptors sites in the carp brain. the radioligand was distributed with an high degree of anatomical selectivity. we found abundant labelling in the cerebellum, in the nucleus diffusus lobi inferioris, and in the torus longitudinalis. no labelling was detected within the epithalamus, thalamus and hypothalamus, while the telencephalon and the rhombencephalon displayed a low density of [(3)h]muscimol receptor ... | 2007 | 17553715 |
| evidence for maternal inheritance of mitochondrial dna in allotetraploid. | the complete mitochondrial dna (mtdna) sequences of the allotetraploid and red crucian carp were determined in this paper. we compared the complete mtdna sequences between the allotetraploid and its female parent red crucian carp, and between the allotetraploid and its male parent common carp. the results indicated that the complete mtdna nucleotide identity (99.7%) between the allotetraploid and its female parent red crucian carp was higher than that (89.0%) between the allotetraploid and its m ... | 2007 | 17541829 |
| corticotropin-releasing factor (crf) and crf-binding protein expression in and release from the head kidney of common carp: evolutionary conservation of the adrenal crf system. | corticotropin-releasing factor (crf) plays a central role in the regulation of the stress axis. in mammals, crf as well as its receptors and its crf-binding protein (crf-bp) are expressed in a variety of organs and tissues outside the central nervous system. one of these extrahypothalamic sites is the adrenal gland, where the paracrine actions of adrenal crf influence cortical steroidogenesis and adrenal blood flow. although the central role of crf signaling in the initiation and regulation of t ... | 2007 | 17535873 |
| the fine structural organization of the olfactory epithelium of cyprinus carpio (linnaeus): a scanning electron microscopic study. | the fine anatomical structures of the olfactory epithelium of cyprinus carpio (linnaeus) have been systematically studied with the help of the scanning electron microscope (sem). the olfactory rosette is an oval structure composed of a number of lamellae arranged on a median raphe. a large part of the lateral surface of the rosette is covered with non-receptor epithelium, whereas the receptor epithelium occupies a much smaller area in the middle part. the nonreceptor epithelium is covered with a ... | 2007 | 17533588 |
| the effects of anticoagulants on hematological indices and blood cell morphology of common carp (cyprinus carpio l.). | hematological parameters (ht, hb, rbc, wbc, plt), erythrocyte size, and osmotic fragility, differential leukocyte count, ros production in common carp blood collected on three anticoagulants: heparin (10 iu/ml, na2edta (0.1, 0.5, and 1 mg/ml), and sodium citrate (0.3 mg/ml) were compared. na2edta caused partial blood hemolysis in ht tubes which made ht measurement impossible, and resulted in high variability of the results. both, citrate and na2edta increased sensitivity of red blood cells to he ... | 2007 | 17509941 |
| the formation of the polyploid hybrids from different subfamily fish crossings and its evolutionary significance. | this study provides genetic evidences at the chromosome, dna content, dna fragment and sequence, and morphological levels to support the successful establishment of the polyploid hybrids of red crucian carp x blunt snout bream, which belonged to a different subfamily of fish (cyprininae subfamily and cultrinae subfamily) in the catalog. we successfully obtained the sterile triploid hybrids and bisexual fertile tetraploid hybrids of red crucian carp (rcc) (female symbol) x blunt snout bream (bsb) ... | 2007 | 17507678 |
| effects of copper sulfate (cuso4) on the levels of glucose and cortisol in common carp, cyprinus carpio. | the objective of this study was to evaluate the possible effects of cuso4 exposure on variations of glucose and cortisol levels in cyprinus carpio. three replicates of 6 fish were subjected to two sub-lethal concentrations of cuso4 (0.16 and 0.53 mg l(-1)) for 14 and 21 days. blood samples were isolated from the fish following the exposure, to measure the levels of cortisol and glucose compared to the control group. the results showed significant increases (p < 0.05) in cortisol levels for both ... | 2007 | 19086513 |
| organization of the torus longitudinalis in the rainbow trout (oncorhynchus mykiss): an immunohistochemical study of the gabaergic system and a dii tract-tracing study. | the torus longitudinalis (tl) is a tectum-associated structure of actinopterygian fishes. the organization of the tl of rainbow trout was studied with nissl staining, golgi methods, immunocytochemistry with antibodies to gamma-aminobutyric acid (gaba), glutamic acid decarboxylase (gad), and the gaba(a) receptor subunits delta and beta2/beta 3, and with tract tracing methods. two types of neuron were characterized: medium-sized gabaergic neurons and small gaba-negative granule cells. gaba(a) rece ... | 2007 | 17492628 |
| complex physiological traits as biomarkers of the sub-lethal toxicological effects of pollutant exposure in fishes. | complex physiological traits, such as routine aerobic metabolic rate or exercise performance, are indicators of the functional integrity of fish that can reveal sub-lethal toxicological effects of aquatic pollutants. these traits have proved valuable in laboratory investigations of the sub-lethal effects of heavy metals, ammonia and various xenobiotics. it is not known, however, whether they can also function as biomarkers of the complex potential range of effects upon overall functional integri ... | 2007 | 17475615 |
| an optical immunosensor for rapid vitellogenin detection in plasma from carp (cyprinus carpio). | vitellogenin (vtg) has proven to be a sensitive and simple biomarker for assessing exposure of fish to environmental estrogens. the aim of this work was to develop a rapid, in the order of minutes, screening method for the detection of fish vtg. the surface plasmon resonance technique (biacore xtrade mark) was coupled with immunodetection for the determination of fish vtg in plasma and mucus from carp (cyprinus carpio). monoclonal anti-vtg antibodies were linked on the sensor surface through che ... | 2007 | 19071687 |
| interpreting physiological responses to environmental change through gene expression profiling. | identification of differentially expressed genes in response to environmental change offers insights into the roles of the transcriptome in the regulation of physiological responses. a variety of methods are now available to implement large-scale gene expression screens, and each method has specific advantages and disadvantages. construction of custom cdna microarrays remains the most popular route to implement expression screens in the non-model organisms favored by comparative physiologists, a ... | 2007 | 17449823 |
| establishment of the diploid gynogenetic hybrid clonal line of red crucian carp x common carp. | this study investigated the gynogenetic cytobiological behavior of the third gynogenetic generation (g(3)), which was generated from the diploid eggs produced by the second gynogenetic generation (g(2)) of red crucian carp x common carp, and determined the chromosomal numbers of g(3), g(2)xscatter scale carp and g(2)xallotetraploid hybrids of red crucian carp x common carp. the results showed that the diploid eggs of g(2) with 100 chromosomes, activated by uv-irradiated sperm from scatter scale ... | 2007 | 17447025 |
| does seston size structure reflect fish-mediated effects on water quality in a degraded semiarid wetland? | this study evaluates whether the size structure of seston (the sum of living and nonliving particles in the water column) reflects the effects of fish on wetland water quality. using enclosures, we measured water quality and zooplankton community structure in the presence and absence of two fish species with distinct foraging strategies [benthivorous carp (cyprinus carpio) and planktivorous mosquitofish (gambusia holbrooki)]. seston collected from the enclosures was counted and sized automatical ... | 2007 | 16957857 |
| [modification of serum proteins in carp during hibernation]. | 2007 | 13499462 | |
| [infection of carp with neoechinorhynchus rutili acanthocephala]. | 2007 | 5085913 | |
| fine structures of the carp tectum opticum. | 2007 | 5525146 | |
| [biology of khawia japonensis (caryophyllidea, cestoda), a parasite of the amur carp]. | the development of the cestode khawia japonensis (yamaguti, 1934) has been studied experimentally. at 18 to 26 c, in 12-14 days oncospheres develop in eggs, whose infective properties manifest themselves in 30-31 days. in the caudal segments of limnodrilus udekemianus they penetrate the body cavity through the intestinal wall and migrate to the anterior margin as they mature. in 55 to 60 days (at the same temperature) mature proceroids become localized in 9-12 segments. the development of embryo ... | 2007 | 733320 |
| [new species of the genus myxobolus (myxosporidia, myxobolidae) from the gill filaments of the carp]. | 2007 | 160029 | |
| [the manifestation of valiporiasis in cultivated fishes]. | lethal cases of valiporiasis in the silver carps of the age 3 and 4 years are described. the resistance of hybrids between different forms of cyprinus carpio to the infection with valipora campylancristrota is discussed. | 2007 | 10862401 |
| [on the development of body-wall musculature diplostomum chromatophorum metacercariae (trematoda: diplostomidae)]. | the body-wall musculature of invasive cercariae and metacercariae of diplostomum chromatophorum at different intervals after the penetration into the experimental intermediate host cyprinus carpio (1, 3, 5, 6, 7, 10, 12, 20, 34, 40 days) has been investigated with the help of tem technique. during the first 10 days after the invasion (in conditions of our experiment), the cercarial subtegumental muscle fibres degenerate. these muscles are replaced by newly formed ones. mass differentiation of my ... | 2007 | 11881137 |
| [the manifestation of immunity in ichthyophthiriasis in carp in maritime territory]. | 2007 | 9479386 | |
| [the distribution of lernaea cyprinacea (copepoda: lernaeidae) in a crucian carp population]. | differences in the infection with copepods and the character of distribution of occurrence frequencies of lernaea in crucian carp depending on the body length and sex of the latter are shown. the lowest infection rate with copepods was recorded for fishes with body length 8-11 cm as compared to specimens with body length 12-18 and 18.5-34 cm. the two latter groups did not differ from each other in this index. in males and females whose body length is 8-11 cm as well as in females with body lengt ... | 2007 | 8474768 |
| carps enhance p53 turnover by degrading 14-3-3sigma and stabilizing mdm2. | carp1 and carp2 proteins (carps) are e3 ligases that target p53 as well as phospho-p53 for degradation. because mdm2 is a critical regulator of p53 turnover, we investigated and found that carps associate with mdm2. we provide evidence that carps stabilize mdm2 by inhibiting mdm2 self-ubiquitination. carps together with mdm2 enhance p53 degradation, thereby inhibiting p53-mediated cell death. carp protein levels correlate with mdm2 levels including under hypoxia where both are reduced. carp2 was ... | 2008 | 18382127 |
| exposure of mother-child and postpartum woman-infant pairs to ddt and its metabolites in tianjin, china. | 1,1,1-trichloro-2,2'-bis(4-chlorophenyl)ethane (ddt) exhibits its long persistence in the environment, unusual bioaccumulation, effects on wildlife, and the possibility of long-term adverse effects on human health, especially reproductive toxicity. despite the prohibition of most persistent organochlorine pesticides in china, the presence of organochlorine residue, including ddt, has been widely indicated in environmental substance. however, scarce information is available about accumulative lev ... | 2008 | 18387653 |
| vitellogenin, a biomarker for environmental estrogenic pollution, of reeves' pond turtles: analysis of similarity for its amino acid sequence and cognate mrna expression after exposure to estrogen. | vitellogenin (vtg), a biomarker for environmental estrogenic pollution, can be detected in the bloodstream of oviparous animals before morphological and functional abnormalities appear due to exposure to environmental estrogens. reports observing vtg in turtles have been limited. we therefore cloned and sequenced a partial cdna of vtg in reeves' pond turtle, chinemys reevesii. the cloned cdna fragment possessed the start codon and 2,229 bp, encoding 743 amino acid residues. a sequence of deduced ... | 2008 | 18388421 |
| pathogenic potential of a collagenase gene from aeromonas veronii. | the role of collagenase as a mechanism of bacterial pathogenicity in some pathogenic bacteria has been reported. the information on the role of collagenase in aeromonas spp. pathogenesis is scant. in the present study, a mutant aeromonas veronii ry001 that is deficient in the putative collagenase gene acg was constructed and compared with the wild-type strain for virulence factors. bacterial cells and cell-free extracellular products of the mutant had significantly less collagenolytic activity, ... | 2008 | 18388966 |
| differential contribution of neutrophilic granulocytes and macrophages to nitrosative stress in a host-parasite animal model. | tyrosine nitration is a hallmark for nitrosative stress caused by the release of reactive oxygen and nitrogen species by activated macrophages and neutrophilic granulocytes at sites of inflammation and infection. in the first part of the study, we used an informative host-parasite animal model to describe the differential contribution of macrophages and neutrophilic granulocytes to in vivo tissue nitration. to this purpose common carp (cyprinus carpio) were infected with the extracellular blood ... | 2008 | 18406465 |
| strain-associated virulence factors of streptococcus iniae in hybrid-striped bass. | streptococcus iniae is a major fish pathogen producing invasive infections that result in economic losses in aquaculture. development of in vitro models of s. iniae virulence may provide insight to the pathogenesis of infection in vivo. three s. iniae strains (k288, 94-426, and 29178) were tested for virulence in a hybrid-striped bass (hsb) model using intraperitoneal injection. s. iniae strains k288 and 94-426 caused high levels of mortality in hsb (lethal dose 2x10(5)cfu) while strain 29178 wa ... | 2008 | 18406547 |
| [use of properties and regulation peculiarities of enzymes of glycogenolysis in fish skeletal muscle depending on peculiarities of motor activity of species]. | levels of activity, properties, and peculiarities of activation of glycogen phosphorylase (gp; ec 2.4.1.1) and glycogen phosphorylase kinase (gpk; ec 2.7.1.38) were studied in the white skeletal muscle of fish differing in motor behavior. no differences in the gp and gpk activity levels were revealed in laskir diplodus annularis (l.), horse mackerel trachurus mediterraneus ponticus, salmon salmo trutta morphario, scorpena scorpaena porcus, scophtalnus maeoticus, and carp cyprinus carpio; however ... | 2008 | 18411509 |
| analysis of dbc1 and its homologs suggests a potential mechanism for regulation of sirtuin domain deacetylases by nad metabolites. | deleted in breast cancer-1 (dbc1) and its paralog carp-1 are large multi-domain proteins, with a nuclear or perinuclear localization, and a role in promoting apoptosis upon processing by caspases. recent studies on human dbc1 show that it is a specific inhibitor of the sirtuin-type deacetylase, sirt1, which deacetylates histones and p53. using sensitive sequence profile searches and hmm-hmm comparisons we show that the central conserved globular domain present in the dbc1 and it homologs from di ... | 2008 | 18418069 |
| production of nitric oxide by carp (cyprinus carpio l.) kidney leukocytes is regulated by cyclic 3',5'-adenosine monophosphate. | the inducible nitric oxide synthase (inos) plays a central role in the inflammatory reactions that follow infection or tissue damage. induction of nitric oxide (no) synthesis by bacterial lipopolysaccharide (lps) depends on activation of g protein-coupled receptors in mammals. thus, it was our intention to evaluate whether similar mechanisms are involved in inos activation in fish leukocytes. therefore, the participation of membrane-bound receptors which activate effectors via g proteins has bee ... | 2008 | 18424130 |
| application of pcr-rf-sscp to study major histocompatibility class ii b polymorphism in common carp (cyprinus carpio l.). | a variety of methods have been applied for the characterization of major histocompatibility (mh) polymorphism in fish. we optimized a technique designated polymerase chain reaction-restriction fragments-single strand conformation polymorphism (pcr-rf-sscp) for screening a large number of individuals for the cyca-dab1 and cyca-dab2 genes polymorphism in common carp. the advantages of this technique are simplicity, high sensitivity and low costs. pcr-rf-sscp analysis revealed different genotypes c ... | 2008 | 18430588 |
| perfluorooctane sulfonate (pfos) and other fluorochemicals in fish blood collected near the outfall of wastewater treatment plant (wwtp) in beijing. | perfluorinated compounds (pfcs) were measured in zooplankton and five fish species collected from gaobeidian lake, which receives discharge from wastewater treatment plant (wwtp) in beijing, china. the mean total pfcs in five fish were in the order: crucian carp>common carp>leather catfish>white semiknife carp>tilapia. perfluorooctane sulfonate (pfos) occurred at the greatest concentrations, with mean concentrations ranging from 5.74 to 64.2 ng/ml serum. perfluorodecanoic acid (pfda) was the sec ... | 2008 | 18439735 |
| meichroacidin containing the membrane occupation and recognition nexus motif is essential for spermatozoa morphogenesis. | meichroacidin (mca) is a highly hydrophilic protein that contains the membrane occupation and recognition nexus motif. mca is expressed during the stages of spermatogenesis from pachytene spermatocytes to mature sperm development and is localized in the male meiotic metaphase chromosome and sperm flagellum. mca sequences are highly conserved in ciona intestinalis, cyprinus carpio, and mammals. to investigate the physiological role of mca, we generated mca-disrupted mutant mice; homozygous mca mu ... | 2008 | 18453535 |
| expression of an adenovirus encoded reporter gene and its reactivation following uvc and oxidative damage in cultured fish cells. | recombinant human adenovirus, adca35lacz, was used to examine expression of a reporter gene and its reactivation following uvc (200-280 nm) and oxidative damage in fish cells. | 2008 | 18470745 |
| extensive expansion and diversification of the chemokine gene family in zebrafish: identification of a novel chemokine subfamily cx. | the chemokine family plays important roles in cell migration and activation. in humans, at least 44 members are known. based on the arrangement of the four conserved cysteine residues, chemokines are now classified into four subfamilies, cxc, cc, xc and cx3c. given that zebrafish is an important experimental model and teleost fishes constitute an evolutionarily diverse group that forms half the vertebrate species, it would be useful to compare the zebrafish chemokine system with those of mammals ... | 2008 | 18482437 |
| family association between immune parameters and resistance to aeromonas hydrophila infection in the indian major carp, labeo rohita. | seven innate immune parameters were investigated in 64 full-sib families (the offspring of 64 sires and 45 dams) from two year-classes of farmed rohu carp (labeo rohita). survival rates were also available from aeromonas hydrophila infection (aeromoniasis) recorded in controlled challenge tests on a different sample of individuals from the same families. due to strong confounding between the animal additive genetic effect and the family effects (common environmental+non-additive genetic), reliab ... | 2008 | 18486488 |
| protein expression by aeromonas hydrophila during growth in vitro and in vivo. | expression of aeromonas hydrophila cellular and extracellular products (ecps) was examined following culture of the bacterium in vitro, in tryptic soy broth (tsb), and in vivo, in dialysis tubing placed within the peritoneal cavity of common carp (cyprinus carpio l.). whole cell (wc), outer membrane proteins (omps) and ecp components of the bacteria were analysed by 1 dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis (1d sds-page). additionally, 2d sds-page was used to analy ... | 2008 | 18490132 |
| recombinant novel pituitary adenylate cyclase-activating polypeptide from african catfish (clarias gariepinus) authenticates its biological function as a growth-promoting factor in low vertebrates. | nowadays, the studies of pituitary adenylate cyclase-activating polypeptide (pacap)-related peptide (prp) and pacap in non-mammalian vertebrates, especially in fish, have paid attention mainly to the localization, cloning, and structural evolution of the peptides, but very little is known about its biological functions as growth-promoting factors in low vertebrates. in this work, we have cloned and characterized the prp/pacap cdna from the commercially important north african catfish clarias gar ... | 2008 | 18492822 |
| nitric oxide formation from the reaction of nitrite with carp and rabbit hemoglobin at intermediate oxygen saturations. | the nitrite reductase activity of deoxyhemoglobin has received much recent interest because the nitric oxide produced in this reaction may participate in blood flow regulation during hypoxia. the present study used spectral deconvolution to characterize the reaction of nitrite with carp and rabbit hemoglobin at different constant oxygen tensions that generate the full range of physiological relevant oxygen saturations. carp is a hypoxia-tolerant species with very high hemoglobin oxygen affinity, ... | 2008 | 18494799 |
| transcriptional analysis of the common carp (cyprinus carpio l.) immune response to the fish louse argulus japonicus thiele (crustacea: branchiura). | in the present study we investigated changes in transcription levels of a panel of selected immune relevant genes in peripheral blood leucocytes (pbl) and skin samples collected from carp exposed to larval argulus japonicus. we show that in skin up-regulation of gene transcription of the chemokine cxca, and to a lesser extent the chemokine receptor cxcr1 and the cytokine tnfalpha, are good indicators of parasite-induced skin damage at 2 days post-parasite exposure. up-regulation of gene transcri ... | 2008 | 18502149 |
| cdna expression library screening and identification of two novel antigens: ubiquitin and receptor for activated c kinase (rack) homologue, of the fish parasite trypanosoma carassii. | trypanosoma carassii is a kinetoplastid parasite infecting cyprinid fish with a high prevalence in nature. antibodies have been shown to play a protective role in the immune response against this parasite in common carp, cyprinus carpio. to identify immunogenic and putative protective t. carassii antigens we constructed a lambdatriplex2 expression library of the parasite and screened this with pooled carp immune serum collected 6 weeks post-infection. screening of the library not only revealed r ... | 2008 | 18502151 |
| antibody response and resistance of cyprinus carpio immunized with cyprinid herpes virus 3 (cyhv-3). | cyhv-3 (cyprinid herpesvirus-3) is a large dna virus that causes a fatal disease in koi and common carp. infection with wild type or attenuated virus induces an immune response that renders the fish resistant to further virus challenges. the kinetics and affinity of the antibody response in immune fish depend on the temperature of the water. virus-inoculated fish produce anti-cyhv-3 antibodies, which gradually decrease during 280 days post infection to a level slightly above that of naïve fish. ... | 2008 | 18534721 |
| carp vitellogenin detection by an optical waveguide lightmode spectroscopy biosensor. | a label-free carp vitellogenin sensor has a strong potential for on-site monitoring on the possible contamination of edible fish with endocrine disruptors as a sum parameter in an inland carp farm. in this study, we performed a sensitive detection for carp vitellogenin with a direct-binding optical waveguide lightmode spectroscopy-based immunosensor. carp vitellogenin bound over the sensor surface quite specifically, judging from the sensor responses according to stepwise antibody immobilization ... | 2008 | 18534837 |
| serum crp-like protein profile in common carp cyprinus carpio challenged with aeromonas hydrophila and escherichia coli lipopolysaccharide. | the potential of c-reactive protein (crp)-like proteins to be used as a biomarker of health status in cultured carp obtained from various european fish lines has been assessed. varying crp-like protein levels in the serum of carp were monitored using an indirect competitive enzyme-linked immunosorbent assay. crp-like protein basal levels in normal fish varied between carp lines, ranging on average from 2.9+/-0.15 to 12.57+/-1.19 microg ml(-1). serum levels of crp-like protein in carp were observ ... | 2008 | 18538390 |
| teleostean il11b exhibits complementing function to il11a and expansive involvement in antibacterial and antiviral responses. | interleukin 11 is a class-1 helical cytokine, having the four-helix bundle structure, possessing pleiotropic characteristics involved in physiological processes including blood production, bone formation and placentation. the interleukin 11 paralogues (il11a and il11b) have been identified in fish with only il11a from carp and trout have been characterized and analyzed for its expression thus far. here, we cloned and studied the structure and expression of il11b in japanese flounder (paralichthy ... | 2008 | 18538848 |
| acute effects of microcystins on the transcription of antioxidant enzyme genes in crucian carp carassius auratus. | recent evidences suggested that oxidative stress may play a significant role in the pathogenesis of mcs toxicity. in the present study, the acute effects of microcystins on the transcription of antioxidant enzyme genes were investigated in liver of crucian carp i.p.-injected with 50 mug mc-lreq per kg body weight (bw). we reported the cdna sequences for four kinds of antioxidant enzyme (gsh-px, cat, cu/zn sod, and gr) genes, and evaluated the oxidant stress induced by mcs through analyzing the t ... | 2008 | 18214919 |
| stimulation of salmon calcitonin on secretion of 17beta-estradiol by the ovarian follicles of common carp, cyprinus carpio. | the effects of salmon calcitonin (sct) on the secretion of 17beta-estradiol (e(2)) were examined in female common carp, cyprinus carpio. vitellogenic stage fish adapted to high-ca water were i.p. injected with vehicle, sct, human chorionic gonadotropin (hcg), or hcg plus sct. to determine whether ovarian follicles are equipped with ct receptors, a ct binding assay was conducted. in the in vitro experiments, vitellogenic follicles were incubated with stimulators and inhibitors. administration of ... | 2008 | 18252964 |
| transcriptional deregulation and a missense mutation define ankrd1 as a candidate gene for total anomalous pulmonary venous return. | total anomalous pulmonary venous return (tapvr) is a congenital heart defect in which the pulmonary veins fail to enter the left atrium and drain instead into the right atrium or one of its venous tributaries. although a genetic basis for tapvr has long been recognized, no single gene involved in the pathogenesis of this disease has been identified to date. we previously reported a tapvr patient bearing a de novo 10;21 balanced translocation. in this work, we cloned both translocation breakpoint ... | 2008 | 18273862 |
| molecular cloning and functional characterisation of a cathepsin l-like proteinase from the fish kinetoplastid parasite trypanosoma carassii. | trypanosoma carassii is a fish kinetoplastid parasite that belongs to the family trypanosomatida. in the present study we cloned a cathepsin l-like proteinase from t. carassii. the nucleotide sequence of 1371bp translated into a preproprotein of 456 amino acids. the preproprotein contained the oxyanion hole (gln), the active triad formed by cys, his and asn and the conserved erfnin-like, gnfd and gcngg motifs, characteristic for cathepsin l proteinases. phylogenetic analysis showed that the t. c ... | 2008 | 18162408 |
| the cloning of dmc1 cdnas and a comparative study of its expression in different ploidy cyprinid fishes. | dmc1 (disrupted meiotic cdna) is a functionally specific gene, which was firstly discovered in yeast and then found to encode a protein required for homologous chromosome synapsis during the process of meiosis. in this investigation, we cloned the partial cdnas of dmc1 of diploid red crucian carp, japanese crucian carp, common carp, triploid crucian carp and allotetraploid hybrids by using a pair of degenerate primers based on the conservative sequence of amino acids of the dmc1 protein in yeast ... | 2008 | 18176789 |
| mitochondrial dna analysis reveals cryptic large-scale invasion of non-native genotypes of common carp (cyprinus carpio) in japan. | wild common carp (cyprinus carpio) are probably suffering from biological invasions of conspecific domesticated strains. however, such invasions may be largely camouflaged by morphological similarities between introduced and native strains. we conducted a large survey of mitochondrial dna sequences (complete d-loop region) from 11 localities in japan. from a total of 166 individuals, 28 haplotypes were determined to fit into six divergent clades. one of the six clades included 19 closely related ... | 2008 | 18194166 |
| genomic sequence of mandarin fish rhabdovirus with an unusual small non-transcriptional orf. | the complete genome of mandarin fish siniperca chuatsi rhabdovirus (scrv) was cloned and sequenced. it comprises 11,545 nucleotides and contains five genes encoding the nucleoprotein n, the phosphoprotein p, the matrix protein m, the glycoprotein g, and the rna-dependent rna polymerase protein l. at the 3' and 5' termini of scrv genome, leader and trailer sequences show inverse complementarity. the n, p, m and g proteins share the highest sequence identities (ranging from 14.8 to 41.5%) with the ... | 2008 | 18068257 |
| transcription of signal-3 cytokines, il-12 and ifn alpha beta, coincides with the timing of cd8 alpha beta up-regulation during viral infection of common carp (cyprinus carpio l). | mammalian naïve cd8+ t cells are activated by antigen (signal 1) and cd28 costimulation (signal 2) to undergo several rounds of cell division, but programming for survival, effector function and memory requires a third signal that can be provided by il-12 and/or type i interferons. functional studies indicate that the route of antigen presentation and costimulation are conserved from fish to mammals. however, the potential of il-12 and ifn alpha beta to act as signal-3 cytokines in infections in ... | 2008 | 18022233 |
| approach for extrapolating in vitro metabolism data to refine bioconcentration factor estimates. | national and international chemical management programs are assessing thousands of chemicals for their persistence, bioaccumulative and environmental toxic properties; however, data for evaluating the bioaccumulation potential for fish are limited. computer based models that account for the uptake and elimination processes that contribute to bioaccumulation may help to meet the need for reliable estimates. one critical elimination process of chemicals is metabolic transformation. it has been sug ... | 2008 | 17904615 |
| administration of recombinant parasite beta-tubulin to goldfish (carassius auratus l.) confers partial protection against challenge infection with trypanosoma danilewskyi laveran and mesnil, 1904. | the infection of carp and other cyprinid fish with trypanosoma danilewskyi was reported to cause significant morbidity and mortality in aquaculture. tubulin is a component of parasite excretory/secretory (es) products recognized by antibodies present in the serum of recovered hosts. to assess the role of parasite tubulin in the induction of a protective immune response in the goldfish, recombinant t. danilewskyi beta-tubulin was produced in escherichia coli and used to immunize goldfish against ... | 2008 | 18006235 |
| titin expression in human articular cartilage and cultured chondrocytes: a novel component in articular cartilage biomechanical sensing? | in striated muscle tissues, the giant protein titin acts as a biomechanically active filament system, coupling stress/strain to gene expression. the objective of the study is to show the existence of titin fragments in human articular cartilage, as in diarthodial joints, chondrocytes are also known to sense and respond to stretching. we have surveyed human cultured cartilage collected from adults with osteoarthritis (oa), without oa and from infants with a set of titin antibodies and primer pair ... | 2008 | 17920806 |
| species of environmental mycobacteria differ in their abilities to grow in human, mouse, and carp macrophages and with regard to the presence of mycobacterial virulence genes, as observed by dna microarray hybridization. | there are many species of environmental mycobacteria (em) that infect animals that are important to the economy and research and that also have zoonotic potential. the genomes of very few of these bacterial species have been sequenced, and little is known about the molecular mechanisms by which most of these opportunistic pathogens cause disease. in this study, 18 isolates of em isolated from fish and humans (including strains of mycobacterium avium, mycobacterium peregrinum, mycobacterium chelo ... | 2008 | 17981953 |
| some quality aspects of fish patties prepared from an indian major carp, labeo rohita (ham.). | six different types of fish patties were prepared from de-boned meat of three weight groups (250 500 g, 501-750 g, and 751-1,000 g) of an indian major carp, labeo rohita, using two extenders (boiled potato and corn flour). the weight of the fish and the type of the extender affected the nutritional quality of the patties. cooking lowered the crude protein but increased the total lipid, total soluble sugars, and contents of the patties. cooking yield increased with an increase in the weight of th ... | 2008 | 17852491 |
| antiproliferative effects and apoptosis induction by probiotic cytoplasmic extracts in fish cell lines. | probiotic bacteria are known to exert a wide range of beneficial effects on their animal hosts. control of intestinal homeostasis, inflammation suppression and a reduction in the incidence of cancer all rely on the antiproliferative potential of probiotics. in this paper, we assess the antiproliferative activity of probiotics in two teleost fish cell lines saf-1, a fibroblast cell line and epc, an epithelioma from carp. cells were grown in the presence of cytoplasmic extracts obtained from two b ... | 2008 | 17706899 |
| differential transcription of multiple forms of alpha-2-macroglobulin in carp (cyprinus carpio) infected with parasites. | alpha-2-macroglobulin (a2m) is a non-specific protease inhibitor involved in host defense mechanisms, inhibiting both endogenous and exogenous proteases. it is unique among the plasma anti-proteases with respect to the diversity of proteases that it can inactivate. carp a2m consists of an alpha and beta chain of which the first includes the bioactive regions. previously, three a2m alpha chain sequences were reported for east-asian common carp. we studied a2m alpha chain variability in european c ... | 2008 | 17662386 |
| seasonal variations of heavy metals in some organs of carp (cyprinus carpio l., 1758) from beyşehir lake (turkey). | in this study which was carried out between march 2003 and february 2005 fe, cu, zn, mn, cr, pb and cd contents were determined in muscle, liver and gill of carp (cyprinus carpio l., 1758) caught from beyşehir lake. among the heavy metals analyzed cr, pb and cd were below the detection limit (<0.03). heavy metal concentrations varied significantly depending on the type of the tissue and season. the highest metal concentrations were found in the liver, followed by gill and muscle. heavy metal lev ... | 2008 | 17503200 |
| molecular cloning and expression analysis of t-bet in ginbuna crucian carp (carassius auratus langsdorfii). | in the adaptive immune system of mammals, naive helper t (th) cells differentiate into th1 or th2 cells. the t-box expressed in t cells (t-bet) is a member of a family of t-box transcription factors that regulates the expression of ifn-gamma and plays a crucial role in th1 cell differentiation and cell-mediated immunity. we cloned and sequenced t-bet cdna for the first time from non-mammalian species, ginbuna crucian carp. ginbuna t-bet was composed of 608 predicted amino acids and showed 41.5% ... | 2008 | 17624433 |
| ecotoxicological assessment of water pollution in sariyar dam lake, turkey. | given the effects of environmental pollution and different biotic factors on some important biochemical markers, as enzymes, two fish species inhabiting the sariyar dam lake, turkey have been investigated. ethoxyresorufin o-deethylase, glutathion s-transferase, lactate dehydrogenase, alkaline phosphatase, acid phosphatase, and alanine and aspartate amino transferase activities have been measured in liver samples of cyprinus carpio and capoeta tinca. also, brain acetylcholinesterase and carboxyle ... | 2008 | 17582495 |
| effect of starvation on transcriptomes of brain and liver in adult female zebrafish (danio rerio). | we used microarray and quantitative real-time pcr (qrt-pcr) analyses in adult female zebrafish (danio rerio) to identify metabolic pathways regulated by starvation in the liver and brain. the transcriptome of whole zebrafish brain showed little response to 21 days of starvation. only agouti-related protein 1 (agrp1) significantly responded, with increased expression in brains of starved fish. in contrast, a 21-day period of starvation significantly downregulated 466 and upregulated 108 transcrip ... | 2008 | 18728227 |
| ompa is an adhesion factor of aeromonas veronii, an optimistic pathogen that habituates in carp intestinal tract. | in the present study, we focused on one of the aeromonas veronii isolates that exhibited marked adhesion onto carp intestine and studied its membrane-associated proteins for their possible involvement in mucosal adhesion. | 2008 | 18828791 |
| in vitro search for alternative promoters to the human immediate early cytomegalovirus (ie-cmv) to express the g gene of viral haemorrhagic septicemia virus (vhsv) in fish epithelial cells. | present dna vaccines against fish rhabdoviruses require intramuscular injection (fish-to-fish vaccination) of their g-protein gene under the control of the human immediate early cytomegalovirus (ie-cmv) promoter, while immersion delivery (mass dna vaccination), for instance, by using fish epithelial-specific promoters, would be more practical for aquaculture. to find fish epithelial-specific promoters alternative to the ie-cmv, a comparative study of the effectiveness of different fish promoters ... | 2008 | 18840493 |
| influence of carp intestinal mucus molecular size and glycosylation on bacterial adhesion. | the first step of the pathogenesis of many infectious diseases is the colonisation of the mucosal surface by the pathogen. bacterial colonisation of the mucosal surface is promoted by adherence to high molecular weight mucus glycoproteins. we examined the effect of carp intestinal mucus glycoproteins on the adhesion of different bacteria. the bacteria used were 3 strains of aeromonas hydrophila, and a. salmonicida, edwardsiella tarda and yersinia ruckeri. all bacteria adhered to mucus, but at va ... | 2008 | 18924378 |
| evolution and regulation of the downstream gene of hypoxia-inducible factor-1alpha in naked carp (gymnocypris przewalskii) from lake qinghai, china. | the naked carp (gymnocypris przewalskii) is a native teleost of lake qinghai (altitude, 3.2 km) on the qinghai-tibet plateau in china. hypoxia-inducible factor (hif)-1alpha of gymnocypris przewalskii was cloned and a phylogenetic tree for vertebrate hif-1alpha was constructed. by analysis of maximum likelihood models of codon substitutions for hif-1alpha, three positive sites in the branch lineages of crucian carp, eelpout, and flounder, and a higher proportion of neutral sites in naked carp, an ... | 2008 | 18941827 |
| detection of salmonellae from fish in a natural river system. | sediment, water, and fish gut samples taken at three sites near the headwaters of the san marcos river, texas, were analyzed for salmonellae salmonella spp. by culture and molecular techniques. while enrichment cultures from sediment and water samples from the two uppermost sites were negative for salmonellae in polymerase chain reaction analyses, both sediment and water samples were positive at the downstream site. at all sites, salmonellae were present in the guts of different fishes (e.g., la ... | 2008 | 18942591 |
| the enigmatic role of the ankyrin repeat domain 1 gene in heart development and disease. | it has been proposed that the ankyrin repeat domain 1 (ankrd1) factor (also known as carp) plays a critical role in transcriptional regulation, myofibrillar assembly and stretch sensing during heart development and cardiac insults. ankrd1/carp has also been reported to negatively regulate cardiac gene expression in cell-based promoter-reporter assays. consequently, rapid up-regulation of the ankrd1 gene in myocardium in response to developmental stimuli or pathological insults has tended to be i ... | 2008 | 18956313 |
| plasma biochemical responses of the omnivorous crucian carp (carassius auratus) to crude cyanobacterial extracts. | healthy crucian carp (carassius auratus) were treated by intraperitoneal (i.p.) injection of crude cyanobacterial extracts at two doses, 50 and 200 microg mc-lr equiv kg(-1) bw. high mortality (100%) was observed within 60 h post injection in the high-dose group. in the treated fish, activities of four plasma enzymes, alanine aminotransferase (alt), alkaline phosphatase (alp), aspartate aminotransferase (ast), and lactate dehydrogenase (ldh), all showed substantial increases, with both dose and ... | 2008 | 18958589 |
| changes in plasma thyroid hormones and cortisol levels in crucian carp (carassius auratus) exposed to the extracted microcystins. | the endocrine response of crucian carp injected intraperitoneally with extracted microcystins (mc) was investigated in this study. fish were injected intraperitoneally either with 0.75% nacl (control) and microcystis extract corresponding to 150 and 600 microg microcystins per kg body weight. the plasma levels of triiodothyronine (t(3)), thyroxine (t(4)), free triiodothyronine (ft(3)), free thyroxine (ft(4)), and cortisol were determined at 0, 1, 3, 12, 24, and 48h post-administration of mc-cont ... | 2008 | 18990424 |
| accumulation of heavy metals in freshwater fish in cage aquaculture at cirata reservoir, west java, indonesia. | freshwater fish demand in west java is supported by cage aquaculture in reservoirs. cirata reservoir is one of three cascading hydropower reservoirs built along the citarum river that receives domestic and industrial wastes. water pollution by heavy metals, such as copper and lead, increases the health risk of humans who consume the fish. the study was aimed to evaluate the concentration of copper and lead in fish, specifically on cyprinus carpio, that are widely cultivated in the cirata reservo ... | 2008 | 18991927 |
| halquinol modulated growth, physiology, and protein profile and halquinol residue withdrawal study in the indian major carp catla catla (hamilton). | the aim of the present study was to evaluate the effect of halquinol, an antimicrobial used as a growth promotor in poultry, on the fresh water fish catla catla in terms of growth promotion, protein profile, and physiology as the rate of oxygen consumption. a synergic increment in the free amino acid level and total protein concentration suggested enhanced anabolic metabolism resulting in weight gain. when compared with an untreated control group, fishes treated with 0.1% halquinol (t1) showed a ... | 2008 | 19024792 |
| behavioral and respiratory dysfunction in the freshwater fish, labeo rohita (hamilton) under malathion intoxication. | a short term definitive test by static renewal bioassay method was conducted to determine the acute toxicity (lc50) of the commercial grade organophosphate insecticide malathion (50% ec) in the freshwater edible fish, labeo rohita. carp fingerlings were exposed for 96 h to different concentrations (6.0 to 10.1 microl/l) of malathion. the acute toxicity value was found to be 9.0 microl/l and one tenth of lc50 (0.9 microl/l) was selected for sub acute studies. behavioral patterns and oxygen consum ... | 2008 | 19024933 |
| spatiotemporal trends in fish mercury from a mine-dominated ecosystem: clear lake, california. | clear lake, california, usa, receives acid mine drainage and mercury (hg) from the sulphur bank mercury mine, a u.s. environmental protection agency (u.s. epa) superfund site that was active intermittently from 1873 to 1957 and partially remediated in 1992. mercury concentrations were analyzed primarily in four species of clear lake fishes: inland silversides (menidia beryllina, planktivore), common carp (cyprinus carpio, benthic scavenger/omnivore), channel catfish (ictalurus punctatus, benthic ... | 2008 | 19475924 |
| cloning, characterization and promoter analysis of the common carp igf-ii gene. | insulin-like growth factors (igfs) belong to a family of growth factors with structural homology to proinsulin. up till now, no specific details regarding the transcriptional regulation by autocrine, paracrine or endocrine effector molecules in vivo have been described for the igf-ii gene. this is in big contrast to igf-i gene transcription which has been studied more extensively. to better understand how the igf-ii gene is controlled at the gene transcription level, we have isolated the common ... | 2008 | 18304762 |
| [kinetics of hemoglobin carrying and releasing oxygen.]. | the purpose of the present study was to examine the kinetic process of hemoglobin (hb) carrying and releasing oxygen. under the standard conditions (ph 7.4, p(o2) 20 mmhg, 20 degrees c) the blood samples of chicken, rabbit, frog and carp were equilibrated in oxygen content analyzer with calibrated gas mixture a (0.5% co2 and 99.5% n2). then the blood samples were exposed to gas mixture b (21% o2, 0.5% co2 and 78.5% n2). after equilibration, the blood samples were exposed to gas mixture a again. ... | 2008 | 18288362 |
| effect of endotoxin on the immunity of indian major carp, labeo rohita. | endotoxin, a lipopolysaccharide component of outer cell wall membrane of the gram-negative bacteria is a factor responsible for a number of biological effects including immunostimulatory activities in different animal species including fish. in this study, l. rohita yearlings of weight ranging from 80 to 100g were injected intraperitoneally with 0.5, 1, 2, 5, 10 and 20 eu/fish dose of endotoxin to find out its effect on the immunity. the l. rohita yearlings were found to resist the endotoxin dos ... | 2008 | 18289877 |
| trypanoplasma borreli cysteine proteinase activities support a conservation of function with respect to digestion of host proteins in common carp. | trypanoplasma borreli is an extracellular parasite that is transmitted by a leech vector and is naturally found in the blood of cyprinid fish. high parasitemia and associated severe anemia together with splenomegaly are typical of infection of common carp, cyprinus carpio l. papain-like cysteine proteinases expressed by trypanosome parasites contribute to the pathogenicity of trypanosomes, and are considered an important target for the development of new trypanocidal drugs. t. borreli is a membe ... | 2008 | 18571233 |
| expression of genes involved in excitatory neurotransmission in anoxic crucian carp (carassius carassius) brain. | the crucian carp, carassius carassius, survives months without oxygen. during anoxia it needs to keep energy expenditure low, particularly in the brain, with its high rate of atp use related to neuronal activity. this could be accomplished by reducing neuronal excitability through altered expression of genes involved in excitatory neurotransmission. through cloning and the use of a recently developed real-time rt-pcr approach, with an external rna control for normalization, we investigated the e ... | 2008 | 18593861 |
| effect of botulinum toxin a-induced paralysis and exercise training on mechanosensing and signalling gene expression in juvenile rat gastrocnemius muscle. | intramuscular injections of the paralytic botulinum neurotoxin a (btx) and physical exercise are used in the treatment of chronic spasticity in children with cerebral palsy. we tested whether btx-induced paralysis and/or exercise training would have differential effects on the expression of mechanosensing and signalling genes implicated in the adaptive remodelling of skeletal muscle. juvenile (29-day-old) male rats were injected with btx or saline (nobtx) into the right gastrocnemius and housed ... | 2008 | 18603602 |
| distribution of marine birnavirus in cultured olive flounder paralichthys olivaceus in korea. | surveys of marine birnavirus (mabv) were undertaken in cultured olive flounder paralichthys olivaceus from the south and west coastal areas and jeju in korea during the period january 1999 to april 2007. mabv was detected in all seasons from the fry, juveniles and adult fish from the areas examined. evident cytopathic effects of the virus including rounding and cell lysis were observed in chinook salmon embryo (chse-214) and rainbow trout gonad (rtg-2) cells, but not in fathead minnow (fhm) and ... | 2008 | 18604495 |
| mmts, a new subfamily of tc1-like transposons. | a novel tc1-like transposable element has been identified as a new dna transposon in the mud loach, misgurnus mizolepis. the m. mizolepis tc1-like transposon (mmts) is comprised of inverted terminal repeats and a single gene that codes tc1-like transposase. the deduced amino acid sequence of the transposase-encoding region of mmts transposon contains motifs including dde motif, which was previously recognized in other tc1-like transposons. however, putative mmts transposase has only 34-37% ident ... | 2008 | 18612245 |
| development and evaluation of a one-step loop-mediated isothermal amplification for detection of spring viraemia of carp virus. | spring viraemia of carp virus (svcv) is the causative agent of svc disease. the main aim of our study was to develop a one-step reverse transcription loop-mediated isothermal amplification (rt-lamp) assay for rapid, sensitive and effective detection of svcv. | 2008 | 18631262 |
| long-term hematopoietic reconstitution by transplantation of kidney hematopoietic stem cells in lethally irradiated clonal ginbuna crucian carp (carassius auratus langsdorfii). | the study of hematopoietic stem cells (hscs) has been facilitated by the transplantation of bone marrow cell populations into lethally irradiated mice. it is widely known that hscs have the capacity for long-term and multilineage hematopoietic reconstitution in lethally irradiated hosts. here, we developed a transplantation model system using clonal ginbuna crucian carp (carassius auratus langsdorfii) that were exposed to a lethal dose of x-rays. the minimum lethal dose (mld) of ginbuna was appr ... | 2008 | 18314191 |
| functionality of a bacillus cereus biological agent in response to physiological variables encountered in aquaculture. | the potential of a bacillus cereus isolate (nrrl 100132) as a biological agent for aquaculture has been demonstrated in vitro and in vivo. the functionality of this isolate across a range of physiological conditions, including salinity, ph and temperature, based on rearing of high-value ornamental cyprinus carpio, was investigated. temperature had a significant influence on germination, specific growth rate and increase in cell number of b. cereus in shake-flask cultures, whilst salinity and ph ... | 2008 | 18317748 |
| a liposome immune lysis assay for enrofloxacin in carp and chicken muscle. | a homogenous complement-mediated liposome immune lysis assay (lila) was developed for the determination of enrofloxacin (enro) in carp and chicken muscle. enro was covalently coupled to dppe, and then immobilized onto the surface of liposomes by reverse-phase evaporation method. the performed liposome would be specifically lysed by the sequential additions of anti-enro monoclonal antibody (mab) and guinea pig complement. through a competitive assay format, the performed liposome can be used to d ... | 2008 | 18331861 |
| cloning of the koi herpesvirus genome as an infectious bacterial artificial chromosome demonstrates that disruption of the thymidine kinase locus induces partial attenuation in cyprinus carpio koi. | koi herpesvirus (khv) is the causative agent of a lethal disease in koi and common carp. in the present study, we describe the cloning of the khv genome as a stable and infectious bacterial artificial chromosome (bac) clone that can be used to produce khv recombinant strains. this goal was achieved by the insertion of a loxp-flanked bac cassette into the thymidine kinase (tk) locus. this insertion led to a bac plasmid that was stably maintained in bacteria and was able to regenerate virions when ... | 2008 | 18337580 |