Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| does the light subunit of the gal/galnac specific lectin have a role in the virulence of entamoeba histolytica? | 2000 | 11070299 | |
| entamoeba histolytica: localization of the gal/galnac adherence lectin in experimental amebic liver abscess. | 2000 | 11070300 | |
| animal model of noninvasive entamoeba histolytica infection. | 2000 | 11070301 | |
| characterization of entamoeba histolytica and entamoeba dispar by biological, biochemical, and molecular parameters. | 2000 | 11070303 | |
| morphological changes of mouse liver infected with trophozoites of entamoeba histolytica. | 2000 | 11070304 | |
| genetic differences between entamoeba histolytica and entamoeba dispar. | 2000 | 11070305 | |
| polymorphic dna markers in entamoeba histolytica. | 2000 | 11070306 | |
| rdna variability in strains of entamoeba histolytica and entamoeba dispar. | 2000 | 11070307 | |
| dna sequences homologous to interspersed element of entamoeba histolytica are present in entamoeba dispar. | 2000 | 11070308 | |
| genomic organization of a 7 kb gene cluster from entamoeba histolytica. | 2000 | 11070309 | |
| characterization of a retrotransposon-like repetitive dna in entamoeba histolytica. | 2000 | 11070310 | |
| mcm proteins of entamoeba histolytica. | 2000 | 11070311 | |
| inhibition of neomycin phosphorotransferase expression in entamoeba histolytica with antisense peptide nucleic acid (pna) oligomers. | 2000 | 11070312 | |
| entamoeba histolytica hybrids. | 2000 | 11070313 | |
| cis-elements upregulate the activity of the entamoeba histolytica ehpgp1 gene promoter. | 2000 | 11070314 | |
| the multidrug-resistance phenotype of entamoeba histolytica-transfected trophozoites with the ehpgp5 gene. | 2000 | 11070315 | |
| algorithm to predict minichef electrophoresis patterns of entamoeba histolytica dna. | 2000 | 11070316 | |
| an initial characterization of the 3' untranslated region of the ehpgp5 mrna in entamoeba histolytica. | 2000 | 11070317 | |
| expression of entamoeba histolytica ehpgp5 gene in xenopus laevis oocytes. | 2000 | 11070318 | |
| expression and immunodetection of a p-glycoprotein in emetine-resistant trophozoites of entamoeba histolytica. | 2000 | 11070319 | |
| possible role of the ccaat/enhancer binding protein in the expression regulation of the ehpgp1 multidrug resistance gene in entamoeba histolytica. | 2000 | 11070320 | |
| dna cloning of the entamoeba histolytica prp6 gene: a putative u4/u6 small nuclear ribonucleoprotein particle (snrnp). | 2000 | 11070321 | |
| presence of sequences homologous to the universal minicircle sequence (ums) of trypanosomatids in the dna circles of entamoeba histolytica. | 2000 | 11070322 | |
| dna binding activity and predicted tertiary structure of the tata binding protein of entamoeba histolytica. | 2000 | 11070323 | |
| the tail domain of entamoeba histolytica gef: a guanine nucleotide exchange factor. | 2000 | 11070324 | |
| structural characterization of the 5'-flanking regions of the entamoeba histolytica ehcp112 and ehadh112 genes. | 2000 | 11070325 | |
| isolation and identification of l10 gene from entamoeba histolytica homologous to wilms' tumor suppressor. | 2000 | 11070326 | |
| immunogenicity of an optimized entamoeba histolytica gal-lectin dna vaccine. | 2000 | 11070327 | |
| isolation of phage mimotopes mimicking a protective epitope of gpi-linked proteophosphoglycan antigens of entamoeba histolytica. | 2000 | 11070328 | |
| bacterial expression of a human monoclonal antibody that inhibits in vitro adherence of entamoeba histolytica trophozoites. | 2000 | 11070329 | |
| cloning and functional expression of rpn1, a regulatory-particle non-atpase subunit 1, of proteasome from trypanosoma cruzi. | non-lysosomal protein degradation in eukaryotic cells involves a proteolytic complex referred to as 26s proteasome that consists of a 20s core particle and one or two 19s regulatory particles. we have cloned the gene rpn1 encoding rpnl (regulatory-particle non-atpase subunit 1), one of the largest subunits of proteasome, from trypanosoma cruzi. it contains 2712 bp and encodes 904 amino acid residues with a calculated molecular mass of 98.2 kda and an isoelectric point of 5.2. the predicted amino ... | 2000 | 11071286 |
| a simple and reliable 5'-race approach. | a novel approach for the amplification of cdna ends is described. it requires only minimal amounts of material, a simple cdna synthesis reaction and a single pcr reaction to amplify the desired 5'- or 3'-ends of a certain cdna of interest. it combines the so called capfinder approach with solid phase cdna synthesis, thus almost eliminating background problems usually associated with 5'-race protocols. this approach could be used to generate complete 5'-ends of numerous cdnas using only one cdna ... | 2000 | 11071950 |
| [diagnostic image (8). amebic colitis]. | a 34-year-old woman suffered from progressive abdominal pain and diarrhoea. laparotomy revealed colonic perforation due to amebic colitis. | 2000 | 11072508 |
| the evolution of entamoeba, a cautionary tale. | in addition to its medical importance, the parasite entamoeba histolytica has been studied by evolutionary biologists because of its unusual structural and biochemical features. recent molecular data have revealed that many of our assumptions regarding the relationships of the organism and its relatives have been incorrect. | 2000 | 11081575 |
| acute appendicitis caused by amebiasis. | we report a case of appendicitis caused by amebiasis in a 45-year-old japanese man. he presented to our hospital with bloody stools in june 1998. sigmoidoscopy disclosed erosion, and a biopsy of the erosion showed colitis caused by entamoeba histolytica infection. four months later, he was admitted to our hospital with a small elastic mass and severe pain in the lower quadrant of the abdomen, which was diagnosed as acute appendicitis. he underwent appendectomy. histopathological examination reve ... | 2000 | 11085496 |
| [clinical analysis of 36 cases with amebic liver abscess]. | to investigate the clinical features of amebic liver abscess, the causes of misdignosis and the effect of medical and surgical therapy on patient's prognosis. | 2000 | 12567615 |
| protozoan parasites that infect the gastrointestinal tract. | protozoa that infect the gastrointestinal tract include the deadly parasite entamoeba histolytica;giardia lamblia, the most common cause of waterborne disease outbreaks; and the large group of spore-forming parasites that share a green algae symbiont and a predilection for causing chronic diarrhea in immunocompromised persons. some recent advances in the epidemiology, pathogenesis, diagnosis, and treatment of the diseases caused by these protozoa are discussed in this review. | 2000 | 17024011 |
| everyday and exotic foodborne parasites. | everyday foodborne parasites, which are endemic in canada, include the protozoans entamoeba histolytica, giardia lamblia and cryptosporidium parvum. however, these parasites are most frequently acquired through unfiltered drinking water, homosexual activity or close personal contact such as in daycare centres and occasionally via a food vehicle. it is likely that many foodborne outbreaks from these protozoa go undetected. transmission of helminth infections, such as tapeworms, is rare in canada ... | 2000 | 18159283 |
| efficacy and safety of quinfamide versus secnidazole in the management of amoebic non-dysenteric colitis in children. | to compare the efficacy and safety of single doses of quinfamide and secnidazole in the treatment of amoebic non-dysenteric colitis in children. | 2000 | 23315350 |
| emerging and reemerging intestinal protozoa. | the intestinal protozoa have gained importance to physicians practicing medicine in the united states, canada, and europe during recent years as a result of increasing world travel, the globalization of the world's economy, and the growing number of chronically immunosuppressed people. during the spring of 1996, cyclospora cayetanensis caused diarrhea in approximately 1500 people exposed to guatemalan raspberries. this epidemic recurred in 1997, emphasizing the risks of the global economy and fo ... | 2001 | 17031144 |
| clinical significance of the redefinition of the agent of amoebiasis. | entamoeba histolytica is the pathogenic species of entamoeba that causes amoebic dysentery and other invasive disease. the morphologically similar species, e. dispar, is non-pathogenic and accounts for about 90% of the previously estimated 500 million e. histolytica infections world-wide. because of the recent redefinition of e. histolytica and e. dispar, and the limited number of drugs available to treat amoebiasis, a new approach to treatment of individuals carrying these parasites is necessar ... | 2001 | 17061507 |
| prevalence of clostridium difficile-associated diarrhoea among hospitalized jordanian patients. | we investigated stool specimens of 400 patients at jordan university hospital (300 patients with clinical diarrhoea and 100 controls without diarrhoea) for the presence of clostridium difficile or its toxin. we found a 9.7% prevalence rate of c. difficile or its toxin in stools of patients with diarrhoea. the prevalence of other potential enteric pathogens, such as salmonella spp. (2.3%), shigella spp. (1.0%) and entamoeba histolytica (2.7%), was significantly less. prevalence of c. difficile or ... | 2001 | 15332775 |
| [infective etiology of diarrhea in adults with hiv infection in dakar: a case-control study on 594 patients]. | a survey was conducted in dakar, senegal from may 1997 to may 1999, to identify major types and prevalences of bacteria, parasites, fungi and rotaviruses associated with diarrhea in relation to human immunodeficiency virus serostatus with the aim to provide guidance to physicians for case management. etiologic agents were identified in a case-control study: cases were hiv-infected patients with diarrhea (hiv+ d+) and hiv seronegative patients with diarrhea (hiv- d+); controls were hiv-infected p ... | 2001 | 15773157 |
| membrane-permeabilizing polypeptides of amoebae - constituents of an archaic antimicrobial system. | amoebae may be viewed as primitive, actively phagocytosing eukaryotic cells, many of which use bacteria as a major nutrient source. at a very archaic level, amoebae exert mechanisms which kill bacteria comparable to those found in phagocytic cells of higher organisms. accordingly, it is tempting to suggest that the ancestors of effector cells of the innate immune system were bacteria-feeding amoebae and that their molecular armament is ancient. here, we summarize the characteristics of antimicro ... | 2001 | 16351813 |
| [preliminary study on isolation, purification and hydrolytic activity of cysteine proteinases in entamoeba histolytica]. | to explore the invading mechanism of amebae in lamina porpria and observe the interaction between the cysteine proteinase (cp) of entamoeba histolytica and laminin. | 2001 | 12571943 |
| [detection of dna of toxoplasma gondii in rat by using polymerase chain reaction]. | to study the significance of dna of toxoplasma gondii in peripheral blood. | 2001 | 12571947 |
| the bifunctional entamoeba histolytica alcohol dehydrogenase 2 (ehadh2) protein is necessary for amebic growth and survival and requires an intact c-terminal domain for both alcohol dahydrogenase and acetaldehyde dehydrogenase activity. | the intestinal protozoan pathogen entamoeba histolytica lacks mitochondria and derives energy from the fermentation of glucose to ethanol with pyruvate, acetyl enzyme co-a, and acetaldehyde as intermediates. a key enzyme in this pathway may be the 97-kda bifunctional e. histolytica alcohol dehydrogenase 2 (ehadh2), which possesses both alcohol dehydrogenase (adh) and acetaldehyde dehydrogenase activity (aldh). ehadh2 appears to be a fusion protein, with separate n-terminal aldh and c-terminal ad ... | 2001 | 11274185 |
| identification and characterization of an entamoeba histolytica upstream regulatory element 3 sequence-specific dna-binding protein containing ef-hand motifs. | the hgl5 gene of entamoeba histolytica is negatively regulated through the upstream regulatory element 3 (ure3) dna motif tattctatt. this motif is also present and significant in the function of the e. histolytica fdx gene promoter. a yeast one-hybrid screen was used to identify an e. histolytica cdna encoding a protein (ure3-bp) that recognized this dna motif. analysis of the predicted amino acid sequence demonstrated the presence of two ef-hand motifs but identified no canonical dna binding mo ... | 2001 | 11278344 |
| unusual presentation of amebic liver abscess with thrombocytopenia and splenomegaly. | 2001 | 11285161 | |
| amebiasis. | human amebiasis is caused by the protozoan parasite entamoeba histolytica. invasion of the colonic mucosa by the trophozoite form of this parasite generally results in the clinical syndrome of amebic dysentery, although there is a wide range of clinical presentations. some patients will progress to disseminated disease, with resulting amebic liver abscesses. although it is estimated that 10% of the world's population is infected with e. histolytica, only 1% of these patients will manifest diseas ... | 2001 | 11246030 |
| a role for a galactose lectin and its ligands during encystment of entamoeba. | in the life cycle of entamoeba parasites alternate between the colon-dwelling trophozoite and the infectious cyst forms. the physiologic stimuli that trigger differentiation of trophozoites into cysts remain undefined. on the surface of the human-infecting entamoeba, parasites express a galactose/n-acetylgatactosamine (gal/galnac)-binding lectin, which plays demonstrated roles in contact-dependent lysis of target cells and resistance to host complement. using a reptilian parasite, entamoeba inva ... | 2001 | 11249188 |
| the two phosphofructokinase gene products of entamoeba histolytica. | two phosphofructokinase genes have been described previously in entamoeba histolytica. the product of the larger of the two genes codes for a 60-kda protein that has been described previously as a pyrophosphate (pp(i))-dependent enzyme, and the product of the second, coding for a 48-kda protein, has been previously reported to be a pp(i)-dependent enzyme with extremely low specific activity. here it is found that the 48-kda protein is not a pp(i)-dependent enzyme but a highly active atp-requirin ... | 2001 | 11262402 |
| new tools for genetic analysis of entamoeba histolytica: blasticidin s deaminase and green fluorescence protein. | 2001 | 11267932 | |
| revisiting amebiasis. | 2001 | 11228009 | |
| isolation and characterization of polymorphic dna from entamoeba histolytica. | an important gap in our understanding of the epidemiology of amebiasis is what determines the outcome of entamoeba histolytica infections. to investigate the possible existence of invasive and noninvasive strains as one factor, the ability to differentiate individual isolates of e. histolytica is necessary. two new loci containing internal repeats, locus 1-2 and locus 5-6, have been isolated. each contains a single repeat block with two types of related direct repeats arranged in tandem. souther ... | 2001 | 11230401 |
| identification of enteropathogenic escherichia coli in simian immunodeficiency virus-infected infant and adult rhesus macaques. | enteropathogenic escherichia coli (epec) was recognized as a common opportunistic pathogen of simian immunodeficiency virus-infected rhesus macaques (macaca mulatta) with aids. retrospective analysis revealed that 27 of 96 (28.1%) animals with aids had features of epec infection, and epec was the most frequent pathogen of the gastrointestinal tract identified morphologically. in 7.3% of animals dying with aids, epec represented the sole opportunistic agent of the gastrointestinal tract at death. ... | 2001 | 11230413 |
| specific detection of entamoeba histolytica dna by hemolysin gene targeted pcr. | diagnostic differentiation of pathogenic entamoeba histolytica from non-pathogenic entamoeba dispar is of great clinical importance. we have developed and evaluated a new polymerase chain reaction (pcr) assay (haemo-pcr) based on the novel e. histolytica hemolysin gene hly6. the specificity of this assay was confirmed by analyzing different entamoeba species, faeces samples, human and bacterial dna, and digestion of amplification products with appropriate restriction enzymes. the sensitivity was ... | 2001 | 11230821 |
| getting sense and finding function in protozoa. | 2001 | 11231546 | |
| inhibition of gene expression in entamoeba histolytica with antisense peptide nucleic acid oligomers. | peptide nucleic acids (pnas) may be a potent tool for gene function studies in medically important parasitic organisms, especially those that have not before been accessible to molecular genetic knockout approaches. one such organism is entamoeba histolytica, the causative agent of amebiasis, which infects about 500 million people and is the cause of clinical disease in over 40 million each year, mainly in the tropical and subtropical world. we used pna antisense oligomers to inhibit expression ... | 2001 | 11231555 |
| high prevalence of infection with entamoeba dispar, but not e. histolytica, in captive macaques. | a total of 268 nonhuman primates (20 species) kept in the primate research institute, kyoto university, japan, were surveyed for intestinal amebas. total positive rates as based on the presence of cysts in the stool following formalin-ether sedimentation were as follows: entamoeba histolytica/e. dispar, 53%; e. coli, 34%; e. hartmanni, 34%; iodamoeba buetschlii, 25%; endolimax nana, 8%; and e. chattoni, 3%. positive rates were higher in old world monkeys and lower in new world monkeys. all the 1 ... | 2001 | 11199843 |
| entamoeba histolytica: production of nitric oxide and in situ activity of nadph diaphorase in amebic liver abscess of hamsters. | entamoeba histolytica trophozoites were inoculated into the liver of hamsters and serum nitrate/nitrite levels [expressed as nitric oxide (no) production] were determined at different times during amebic liver abscess (ala) development. we also tested the effects of no synthase (nos) inhibitors such as n(g)-nitro-l-arginine methyl ester (l-name), aminoguanidine, and dexamethasone during ala production. since nos activity has been correlated with expression of reduced nicotinamide adenine dinucle ... | 2001 | 11199849 |
| protection of hamsters from amebic liver abscess formation by immunization with the 150- and 170-kda surface antigens of entamoeba histolytica. | a monoclonal antibody, eh3015, prevents in vitro adherence of entamoeba histolytica trophozoites to mammalian cells and inhibits amebic liver abscess formation in hamsters. by immunoaffinity chromatography with the monoclonal antibody, purified e. histolytica antigens with molecular masses of 150 and 170 kda under non-reduced conditions were obtained. hamsters were immunized with these antigens (group i) or with fractions further purified by polyacrylamide gel electrophoresis (group ii). pooled ... | 2001 | 11206109 |
| synthesis and antiparasitic activity of 2-(trifluoromethyl)-benzimidazole derivatives. | 2-(trifluoromethyl)benzimidazole derivatives substituted at the 1-, 5-, and 6-positions have been synthesized and in vitro tested against the protozoa giardia lamblia, entamnoeha histolytica. and the helminth trichinella spiralis. results indicate that all the compounds tested are more active as antiprotozoal agents than albendazole and metronidazole. one compound (20) was as active as albendazole against t. spiralis. these compounds were also tested for their effect on tubulin polymerization an ... | 2001 | 11206455 |
| the unusual transhydrogenase of entamoeba histolytica. | we have expressed and purified a protein fragment from entamoeba histolytica. it catalyses transhydrogenation between analogues of nad(h) and nadp(h). the characteristics of this reaction resemble those of the reaction catalysed by a complex of the nad(h)- and nadp(h)-binding subunits of proton-translocating transhydrogenases from bacteria and mammals. it is concluded that the complete en. histolytica protein, which, along with similar proteins from other protozoan parasites, has an unusual subu ... | 2001 | 11163794 |
| granulysin: a novel antimicrobial. | granulysin is a novel lytic molecule produced by human cytolytic t-lymphocytes (ctls) and natural killer (nk) cells. it is active against a broad range of microbes, including gram-positive and -negative bacteria, parasites and mycobacterium tuberculosis. it is functionally related to other antibacterial peptides, like defensins and magainins, but is structurally distinct. it has structural similarity to porcine nk-lysin and to amoebapores made by entamoeba histolytica. synthetic peptides derived ... | 2001 | 11178344 |
| a review of plant species assessed in vitro for antiamoebic activity or both antiamoebic and antiplasmodial properties. | the resurgence of the protozoal diseases amoebiasis and malaria has been known to occur, from time to time, in endemic and epidemic proportions all over the world. furthermore, the import of these individual pathogens to other areas from tropical regions encourages these protozoal diseases to occur on a global scale with considerable associated mortality and morbidity. from time immemorial, the cure of these diseases has been attempted with the use of traditional plant products, derived from suc ... | 2001 | 11180515 |
| a novel anti-inflammatory oligopeptide produced by entamoeba histolytica. | the monocyte locomotion inhibitory factor (mlif), a heat-stable oligopeptide found in the supernatant fluid of entamoeba histolytica axenic cultures was isolated by ultra-filtration, gel-sieve chromatography and high powered liquid chromatography (hplc), and its primary structure (met-gln-cys-asn-ser) established by edman sequencing and mass-spectrometry (ms). a synthetic peptide had the same selective anti-inflammatory features as the native material in comparable concentrations: in vitro inhib ... | 2001 | 11223127 |
| the cell cycle of entamoeba invadens during vegetative growth and differentiation. | the cell division cycle of entamoeba invadens was studied during vegetative growth of trophozoites and during their differentiation into cysts. during vegetative growth of trophozoites, it was observed that dna synthesis typically continued after one genome content had been duplicated. during encystation, dna synthesis was arrested after 4n genome content had been synthesised. using multi-parameter flow cytometry, the light scattering properties of cysts and trophozoites were studied. the cytopl ... | 2001 | 11223134 |
| [diagnostic image (8). intestinal amoebiasis]. | 2001 | 11225255 | |
| a plasma membrane-type ca(2+)-atpase co-localizes with a vacuolar h(+)-pyrophosphatase to acidocalcisomes of toxoplasma gondii. | ca(2+)-atpases are likely to play critical roles in the biochemistry of toxoplasma gondii, since these protozoa are obligate intracellular parasites and the ca(2+) concentration in their intracellular location is three orders of magnitude lower than in the extracellular medium. here, we report the cloning and sequencing of a gene encoding a plasma membrane-type ca(2+)-atpase (pmca) of t.gondii (tga1). the predicted protein (tga1) exhibits 32-36% identity to vacuolar ca(2+)-atpases of trypanosoma ... | 2001 | 11226155 |
| overexpression of cysteine proteinase 2 in entamoeba histolytica or entamoeba dispar increases amoeba-induced monolayer destruction in vitro but does not augment amoebic liver abscess formation in gerbils. | to study the role of cysteine proteinases in the pathogenicity of entamoeba histolytica, we have attempted to overexpress the three main cysteine proteinases (ehcp1, ehcp2, ehcp5) of this parasite in trophozoites of e. histolytica as well as in non-pathogenic entamoeba dispar by episomal transfection. although each of the corresponding coding sequences were cloned in identical expression plasmids, we were unable to overexpress ehcp1 and ehcp5, respectively, but could substantially induce express ... | 2001 | 11207616 |
| identification of two entamoeba histolytica sequence-specific ure4 enhancer-binding proteins with homology to the rna-binding motif rrm. | to study transcriptional regulation in the lower branching eukaryote entamoeba histolytica, we have identified two sequence-specific dna-binding proteins that recognize the upstream regulatory element ure4, an enhancer that regulates expression of the gal/galnac lectin heavy subunit gene hgl5. a chromatographic purification of e. histolytica nuclear extracts by gel filtration, cation exchange, and sequence-specific dna affinity chromatography led to a 700-fold increase in ure4 binding activity a ... | 2001 | 11038357 |
| drug targets and mechanisms of resistance in the anaerobic protozoa. | the anaerobic protozoa giardia duodenalis, trichomonas vaginalis, and entamoeba histolytica infect up to a billion people each year. g. duodenalis and e. histolytica are primarily pathogens of the intestinal tract, although e. histolytica can form abscesses and invade other organs, where it can be fatal if left untreated. t. vaginalis infection is a sexually transmitted infection causing vaginitis and acute inflammatory disease of the genital mucosa. t. vaginalis has also been reported in the ur ... | 2001 | 11148007 |
| development of monoclonal antibodies which specifically recognize entamoeba histolytica in preserved stool samples. | we report the generation of monoclonal antibodies against a recombinant 170-kda subunit of the gal or galnac lectin of entamoeba histolytica that specifically recognize e. histolytica but not entamoeba dispar in preserved stool samples. these antibodies do not cross-react with other bowel protozoa, including entamoeba coli, giardia lamblia, and dientamoeba fragilis. | 2001 | 11158133 |
| evaluation of the triage micro parasite panel for detection of giardia lamblia, entamoeba histolytica/entamoeba dispar, and cryptosporidium parvum in patient stool specimens. | a study comparing the triage micro parasite panel (biosite diagnostics, inc., san diego, calif.) to conventional o&p examination (o&p) was performed using patient fecal specimens. five hundred twenty-three stool samples were compared. nineteen specimens were found to be positive by triage, and 29 were found to be positive by o&p. seven specimens were positive for giardia lamblia, four were positive for entamoeba histolytica/e. dispar, and three were positive for cryptosporidium parvum as determi ... | 2001 | 11136793 |
| tropical diarrhoea: new developments in traveller's diarrhoea. | traveller's diarrhoea is the most common illness acquired by visitors to developing countries, affecting 20-50% of the 35 million people who travel from industrialized countries each year. important risk factors include point of origin and destination of the traveller, host factors, and exposure to contaminated food and water. the most common causes of traveller's diarrhoea in adults in developing countries include infection with escherichia coli, shigella spp., salmonella spp., campylobacter sp ... | 2001 | 11964874 |
| parasitic infections of the intestine. | intestinal protozoal infections cause significant disease not only in the tropics but also in immunocompromised hosts and returning travellers in the developed world. precise diagnosis of protozoal intestinal infection by microscopy can be difficult. enzyme immunoassays for antigen detection are being used for some protozoal infections with some limitations which will, it is hoped, be overcome by molecular techniques. nucleic acid amplification techniques could help improve detection of microspo ... | 2001 | 11964880 |
| protozoan enteric infection in aids related diarrhea in thailand. | the aim of this study was to determine the prevalence of enteric protozoa and other pathogens in aids patients with diarrhea in bangkok, thailand. of 288 consecutive patients screened in the 10 month period between november 1999-august 2000 inclusive, 55 (19.2%) had cryptosporidium spp, 13 (4.5%) had isospora oocyst, 11 (3.8%) had giardia lamblia, 3 (0.9%) had entamoeba histolytica, and 1 (0.3%) had iodamoeba butschlii infection. the prevalence of microsporidia was 11% in this study. of 251 pati ... | 2001 | 12041580 |
| diagnosis of intestinal amebiasis using salivary iga antibody detection. | attempts were made to use soluble antigen extract of strain hk-9 of entamoeba histolytica to detect salivary iga antibodies in intestinal amebiasis patients by using elisa. total salivary samples of 109 individuals were divided into four groups. group i comprised 32 patients whose stools were positive only for e. histolytica cysts and/or trophozoites. group ii comprised 12 individuals whose stools were positive for e. histolytica and other intestinal parasites. group iii comprised 36 individuals ... | 2001 | 12041582 |
| comparison between r-phycocyanin-labeled and r-phycoerythrin-labeled monoclonal antibody (mab) probes for the detection of entamoeba histolytica trophozoites. | a comparison between r-phycocyanin (r-pc)-labeled monoclonal antibody (mab) probe and r-phycoerythrin (r-pe)-labeled mab probe for the detection of the three standard reference strains of the cultured-derived entamoeba histolytica trophozoites, namely hk-9, hm-1:imss, and hth-56:mutm were evaluated by using direct immunofluorescence antibody (difa) assay five times for each strain. under the blue irradiation of the fluorescent microscope, both r-pc-labeled and r-pe-labeled mab probes showed cons ... | 2001 | 12041583 |
| [pathogenic protozoans in man: differential characterization of entamoeba histolytica/entamoeba dispar complex, acanthamoeba spp., microsporidia]. | the review summarizes the results in the main parasitological topics of our lab: amoebic infections due to entamoeba histolytica/entamoeba dispar complex and to acanthamoeba spp. respectively, and human infections caused by microsporidia. different rapid and advanced techniques have been included in the standardized diagnostic protocols for each topic, and a critical comparison among them was made, in order to define the gold standard diagnostic method: a) e. histolytica/e. dispar: in vitro cult ... | 2001 | 12078476 |
| asymptomatic amoebic infection: entamoeba histolytica or entamoeba dispar? that is the question. | two cases of amoebic infection were diagnosed in a heterosexual couple. the cases, a frenchman with previous trips to various african countries and his sexual partner, a cameroonese woman immigrant living outside the community, were both asymptomatic; the infection had been diagnosed by chance in the man at the time of his employment in a hospital kitchen. based on what is known of the epidemiology of amoebic infection, it may be acquired and then transmitted within a couple via the indirect fae ... | 2001 | 11845521 |
| entamoeba histolytica: transferrin binding proteins. | entamoeba histolytica trophozoites depend on iron for their growth; thus, they must use some host iron-containing molecules to fulfill this requirement. in this work we report that amoebas are able to utilize human holo-tf as iron source and to recognize it through transferrin binding proteins. by use of an anti-human transferrin antiserum in an immunoblotting assay, two main polypeptides with apparent molecular masses of 70 and 140 kda were found in total extract of trophozoites cultured in vit ... | 2001 | 11846523 |
| diagnostic dilemma in coinfection. | a fifteen years girl belonging to a low socioeconomic status was admitted with peritonsillar abscess caused by methicillin resistant staphylococcus aureus (mrsa), high fever, diarrhoea and septicaemic shock. initial blood cultures and widal test, stool cultures and routine stool examination were non-contributory to the diagnosis. a bone marrow culture in the second week confirmed the diagnosis of salmonella typhi infection. examination of a fresh stool sample showed cysts of entamoeba histolytic ... | 2001 | 11848310 |
| novel putative saposin-like proteins of entamoeba histolytica different from amoebapores. | amoebapores, the pore-forming proteins of entamoeba histolytica, have been shown to play a pivotal role in the pathogenicity of the protozoan parasite. they belong to the functionally diverse family of saposin-like proteins (saplips) characterized by a conserved pattern of cysteine residues and the ability to interact with lipids. here, we report the identification of genomic sequences encoding presumably novel saplips in e. histolytica and classify them in the structural and functional context ... | 2001 | 11513801 |
| identification and characterization of genes encoding novel rab proteins from entamoeba histolytica. | 2001 | 11522354 | |
| characterization of the ehrab8 gene, a marker of the late stages of the secretory pathway of entamoeba histolytica. | 2001 | 11522355 | |
| variability of calcium binding to ef-hand motifs probed by electrospray ionization mass spectrometry. | the modulation of calcium binding by the ef-hand motifs present in a calmodulin (cam) homologue, a calcium binding protein (cabp) from entamoeba histolytica by three external parameters-ph, ligand coordinator egta, and fragmentor voltage was investigated by mass spectrometry. calcium binding follows expected patterns at highly acidic and alkaline ph with the preponderance of the apo and the completely saturated forms, respectively. surprisingly, additional nonspecific binding is observed near ne ... | 2001 | 11766756 |
| case of amoebic liver abscess: prolonged latency or acquired in australia? | 2001 | 11767876 | |
| a rapid-cooling method for cryopreserving entamoeba histolytica. | 2001 | 11784440 | |
| purification and characterization of receptors for myoinositol trisphosphate and myoinositol tetrakis phosphate from entamoeba histolytica. | the microsomal fraction from the log phase of entamoeba histolytica cells contains ins(1,4,5)p3 and ins(1,3,4,5)p4 binding activity. the binding proteins/receptors for both ins(1,4,5)p3 and ins(1,3,4,5)p4 were purified and found to be specific for each ligand. the molecular masses for native proteins for insp3 and insp4 are 138 kda and 130 kda respectively having subunits of 69 kda and 64 kda respectively. that these proteins are associated with ca2+ release was confirmed by including these prot ... | 2001 | 11811621 |
| meeting report: anaerobic protozoan parasites, prague, czech republic, july 15-19, 2001. | 2001 | 11822654 | |
| [chromosome-like bodies of dysentery ameba entamoeba histolytica]. | intact and surface stretched amembraneous nuclei of entamoeba histolytica (rhizopoda, lobosea, entamoebidae) trophozoites were studied by light and electron microscopy. a moderately dense karyosome about 1.5 microns in diameter, localized in the central part of the interphase nucleus, contains the bulk of nuclear dna. within the karyosome, beaded and ribbon-like chromatin bodies surrounding a loose fibrillar core are commonly recognized. the peripheral domain of both interphase and several mitot ... | 2001 | 11840785 |
| entamoeba dispar: molecular characterization of the galactose/n-acetyl-d-galactosamine lectin. | amebiasis contributes to approximately 50 million cases of life-threatening dysentery worldwide. comparison of the lectins from entamoeba histolytica (pathogenic) and entamoeba dispar (nonpathogenic) was undertaken to elucidate the differential roles of this molecule in invasion versus colonization. surface lectin was less abundant on axenic e. dispar than on axenic e. histolytica, commensurate with differences in lectin (heavy and light subunits) rna when assessed by semiquantitative rt-pcr. th ... | 2001 | 11888250 |
| a school waterborne outbreak involving both shigella sonnei and entamoeba histolytica. | in an outbreak of gastroenteritis affecting 730 students, shigella sonnei and entamoeba histolytica were isolated from the stool specimens of patients. environmental investigations revealed the source of infection to be contamination of underground well water by sewage from a toilet. the outbreak ended with the closure of the well water supply. to avoid such problems, institutions and other groups that maintain their own wells, including schools and summer camps, need to be vigilant about mainte ... | 2001 | 11936033 |
| gender variations in the prevalence of parasitic infections and the level of awareness in adolescents in rural nepal. | the study was conducted in rural school adolescent children to investigate the awareness and its association in parasitic infections in boys and girls. of the 182 children examined 119 (65.3%) were male and 63 (34.6%) were female, age ranged 12-20 years with median age 15 years. out of 182 stool samples examined 73 (40%) were found to be positive for parasites in which two or more parasites were found in 10 stool samples. giardia lamblia 33 (18.1%) was the predominant parasite followed by hook w ... | 2001 | 11944720 |
| ordinary and opportunistic enteropathogens associated with diarrhea in senegalese adults in relation to human immunodeficiency virus serostatus. | a survey was conducted in dakar, senegal, to identify major types and prevalences of bacteria, parasites, fungi, and rotaviruses associated with diarrhea in relation to human immunodeficiency virus (hiv) serostatus with the goal to provide guidance to physicians for case management. | 2001 | 11953215 |
| a political asylum seeker with an amoebic empyema in birmingham. | 2001 | 11436450 | |
| purification and identification of major soluble 40-kda antigenic protein from entamoeba histolytica: its application for serodiagnosis of asymptomatic amebiasis. | one of the major soluble antigenic proteins of entamoeba histolytica was purified to homogeneity and identified on a molecular basis. its recombinant protein was expressed in escherichia coli as a fusion protein with shistosoma japonicum glutathione s-transferase. apparent molecular weight of the purified antigenic protein was estimated to be 40-kda and molecular-based analysis indicated that the purified protein was nadp+-dependent alcohol dehydrogenase (ehadh1). the application of the purified ... | 2001 | 11438429 |
| molecular cloning and characterization of peroxiredoxin from toxoplasma gondii. | a cdna of 1.1 kb comprising the gene encoding the peroxiredoxin of toxoplasma gondii (tgprx) has been cloned. the open reading frame of 591 bp was translated into a protein of 196 amino acids with a molecular mass of 25 kda. conserved 2 cysteine domains of phe-val-cys-pro and glu-val-cys-pro indicated tgprx belonged to 2-cys prx families. tgprx showed the highest homology with that of arabidopsis thaliana by 53.9% followed by entamoeba histolytica with 39.5% by the amino acid sequence alignment. ... | 2001 | 11441500 |