Publications

structural and enzymatic analysis of soybean beta-amylase mutants with increased ph optimum.	comparison of the architecture around the active site of soybean beta-amylase and bacillus cereus beta-amylase showed that the hydrogen bond networks (glu380-(lys295-met51) and glu380-asn340-glu178) in soybean beta-amylase around the base catalytic residue, glu380, seem to contribute to the lower ph optimum of soybean beta-amylase. to convert the ph optimum of soybean beta-amylase (ph 5.4) to that of the bacterial type enzyme (ph 6.7), three mutants of soybean beta-amylase, m51t, e178y, and n340 ...	2004	14638688
multiple brain abscesses in an extremely preterm infant: treatment surveillance with interleukin-6 in the csf.		2004	14586650
microbiological quality of ready-to-eat foods: results from a long-term surveillance program (1995 through 2003).	the coordination of food sampling activities across wales, a part of the united kingdom with a population of approximately 3 million, led to the establishment in 1995 of a coordinated food-sampling program designed to monitor on a long-term basis the microbiological quality and safety of specific ready-to-eat products. this surveillance system has been ongoing for 9 years and has generated a database of microbiological and associated demographic results for 15,228 ready-to-eat food samples. the ...	2005	21132974
spore formation by bacillus cereus in broth as affected by temperature, nutrient availability, and manganese.	a study was done to determine the effect of interacting factors on sporulation of bacillus cereus in broth. vegetative cells (1.4 to 2.2 log cfu/ml) of b. cereus strain 038-2 (capable of growing at 12 degrees c) and strain f3812/84 (capable of growing at 8 degrees c) were inoculated into 30 ml of tryptic soy broth (tsb), tsb supplemented with manganese (50 microg/ml), diluted (10%) tsb (dtsb), and dtsb supplemented with manganese (50 microg/ml) and incubated at 8, 12, or 22 degrees c for up to 3 ...	2005	21132988
method for evaluation of the efficacy of antimicrobial preservatives in cosmetic wet wipes.	many cosmetic formulations are now available in the form of wet wipes packaged in sealed sachets or packets. like the majority of cosmetic products having an aqueous phase, wipes are susceptible to microbial contamination and require the addition of preservatives. the efficacy of such preservatives can be evaluated using a standard challenge test performed on the wetting liquid but this test cannot be regarded as representative for this new type of formulation. the method presented here evaluate ...	2005	18492191
biomedical evaluation of two sympatric lemur species (propithecus verreauxi deckeni and eulemur fulvus rufus) in tsiombokibo classified forest, madagascar.	complete medical examinations were performed on 20 wild decken's sifaka (propithecus verreauxi deckeni) and 20 wild red-fronted brown lemurs (eulemurfulvus rufus) from western madagascar. each animal received a complete physical examination, and weight, body temperature, heart rate, and respiratory rate were recorded and ectoparasites collected. blood samples were collected for complete blood cell count, differential white blood cell count, hemoparasite examination, serum biochemical profile, fa ...	2005	17312713
purification and characterization of extracellular 1,2-alpha-l-fucosidase from bacillus cereus.	bacillus cereus isolated from a soil sample, inductively produced alpha-l-fucosidase in culture medium containing porcine gastric mucin (pgm). the production of the enzyme was also weakly induced by l-fucose and d-arabinose, but not by other sugars including glucose. the enzyme was purified 61-fold with an overall recovery of 1.8% from the culture fluid supplemented with pgm by ammonium sulfate precipitation, acetone fractionation, and subsequent column chromatography. the purified enzyme was fo ...	2005	16233842
characterization of sporulation histidine kinases of bacillus anthracis.	the initiation of sporulation in bacillus species is regulated by the phosphorelay signal transduction pathway, which is activated by several histidine sensor kinases in response to cellular and metabolic signals. comparison of the protein components of the phosphorelay between bacillus subtilis and bacillus anthracis revealed high homology in the phosphorelay orthologs of spo0f, spo0b, and spo0a. the sensor domains of sensor histidine kinases are poorly conserved between species, making ortholo ...	2005	16199567
deletion of the sigb gene in bacillus cereus atcc 14579 leads to hydrogen peroxide hyperresistance.	the sigb gene of bacillus cereus atcc 14579 encodes the alternative sigma factor sigma(b). deletion of sigb in b. cereus leads to hyperresistance to hydrogen peroxide. the expression of kata, which encodes one of the catalases of b. cereus, is upregulated in the sigb deletion mutant, and this may contribute to the hydrogen peroxide-resistant phenotype.	2005	16204573
in-vitro antibacterial, antifungal and cytotoxic activities of some coumarins and their metal complexes.	a series of new antibacterial and antifungal coumarin-derived compounds and their transition metal complexes [cobalt (ii), copper (ii), nickel (ii) and zinc (ii)] have been synthesized, characterized and screened for their in vitro antibacterial activity against escherichia coli, klebsiella pneumoniae, proteus mirabilis, pseudomonas aeruginosa, salmonella typhi, shigella dysenteriae, bacillus cereus, corynebacterium diphtheriae, staphylococcus aureus and streptococcus pyogenes bacterial strains ...	2005	16206827
purification of bovine milk lactoperoxidase and investigation of antibacterial properties at different thiocyanate mediated.	bovine lactoperoxidase (lpo) was purified with amberlite cg 50 h+ resin, cm sephadex c-50 ion-exchange chromatography, and sephadex g-100 gel filtration chromatography from skim milk. the activity of lactoperoxidase was measured by using 2.2-azino-bis (3-ethylbenzthiazoline-6 sulfonic acid) diammonium salt (abts) as a choromogenic substrate at ph 6.0. purification degree for the purified enzyme was controlled with sds-page and rz value (a412/a280). rz value for the purified lpo was 0.8. km value ...	2005	16212035
bacillus cereus produces several nonproteinaceous insecticidal exotoxins.	bacillus cereus is mainly known as a human food-borne opportunistic pathogen. here, we used biological assays and hplc to investigate the ability of b. cereus to produce insecticidal exotoxins during the stationary growth phase. none of the 575 b. cereus strains screened produced detectable levels of beta-exotoxin i, a small, heat-stable insecticidal nucleotide analogue. however, six out of a subset of 270 b. cereus strains produced several small, nonproteinaceous insecticidal exotoxins differen ...	2005	16214163
genome sequence and gene expression of bacillus anthracis bacteriophage fah.	fah, a lytic bacteriophage of bacillus anthracis, is used widely in the former soviet union to identify anthrax bacteria. here, we present the analysis of a 37,974 bp sequence of the fah genome and examine gene expression of the phage in a model host, bacillus cereus. half of the fah genome contains genes coding for structural proteins and host lysis functions in an arrangement typical of syphoviridae. the other half of the genome contains genes coding for enzymes of viral genome replication and ...	2005	16226766
stability of enterocin as-48 in fruit and vegetable juices.	enterocin as-48 is a candidate bacteriocin for food biopreservation. before addressing application of as-48 to vegetable-based foods, the interaction between as-48 and vegetable food components and the stability of as-48 were studied. enterocin as-48 had variable interactions with fruit and vegetable juices, with complete, partial, or negligible loss of activity. for some juices, loss of activity was ameliorated by increasing the bacteriocin concentration, diluting the juice, or applying a heat ...	2005	16245711
establishment of a novel multiplex pcr assay and detection of toxigenic strains of the species in the bacillus cereus group.	five different enterotoxins and one emetic toxin of bacillus cereus have been characterized. to amplify all of the enterotoxin and emetic-specific sequences of the species in the b. cereus group, a multiplex pcr with 12 primer pairs was established. in developing the assay method, a common terminal sequence at the 3' ends of all primers was chosen and a hot start taq polymerase was used to overcome primer dimer formation. the assay was successfully applied to analyze the toxigenic potential of 1 ...	2005	16245717
[study of real-time pcr assays for rapid detection of food-borne pathogens].	a duplex real-time sybr green lightcycler pcr (lc-pcr) assay with dna extraction using qiaamp dna stool minikit was evaluated for detection of 8 of 19 species of food-borne pathogens, including plesiomonas shigelloides, providencia alcalifaciens, in five stool specimens. the time frame was within 2h or less. the protocol used the same lc-pcr with 22 pairs of specific primers. the rapid amplification and reliable detection of specific genes were determined by this lc-pcr assay from 10 cases of fo ...	2005	16248373
immunoassay and polymerase chain reaction techniques for detection of enterotoxigenic bacillus cereus.	to compare the reverse passive latex agglutination (rpla) and enzyme linked immunosorbent assay (elisa) techniques with a polymerase chain reaction (pcr) for detection of enterotoxigenic bacillus cereus.	2005	16261920
optimal sampling time after preparation of platelet concentrates for detection of bacterial contamination by quantitative real-time polymerase chain reaction.	a universal quantitative real-time polymerase chain reaction (pcr), based on bacterial 16s rdna, to detect bacterial contamination of platelet concentrates (pcs), was developed previously and compared with automated culturing. in the present study, this real-time pcr method was evaluated to determine the optimal sampling time for screening of bacterial contamination in pcs.	2005	16262753
bacillus cereus var. toyoii and saccharomyces boulardii increased feed efficiency in broilers infected with salmonella enteritidis.	1. the effect on feed efficiency of two probiotics, one prepared with saccharomyces boulardii and the other with bacillus cereus var. toyoii, was tested in broilers infected with salmonella enteritidis. 2. one-day-old chicks were divided at random into three groups and fed commercial feed devoid of antibiotics: group 1 was fed with non-supplemented feed, group 2 was supplemented with s. boulardii and group 3 with b. cereus. at 14 d of age the animals were challenged by the oral route with 1 x 10 ...	2005	16268108
comparison of minisatellite polymorphisms in the bacillus cereus complex: a simple assay for large-scale screening and identification of strains most closely related to bacillus anthracis.	polymorphism of five tandem repeats that are monomorphic in bacillus anthracis was investigated in 230 isolates of the b. cereus group and in 5 sequenced b. cereus genomes in search for markers allowing identification of b. cereus and b. thuringiensis strains most closely related to b. anthracis. using this multiple-locus variable number of tandem repeat analysis (mlva), a cluster of 30 strains was selected for further characterization. eventually, six of these were characterized by multilocus s ...	2005	16269689
photodynamic inactivation of bacillus spores, mediated by phenothiazinium dyes.	spore formation is a sophisticated mechanism by which some bacteria survive conditions of stress and starvation by producing a multilayered protective capsule enclosing their condensed dna. spores are highly resistant to damage by heat, radiation, and commonly employed antibacterial agents. previously, spores have also been shown to be resistant to photodynamic inactivation using dyes and light that easily destroy the corresponding vegetative bacteria. we have discovered that bacillus spores are ...	2005	16269726
investigations on bacteria as a potential biological control agent of summer chafer, amphimallon solstitiale l. (coleoptera: scarabaeidae).	studying the bacteria of hazardous insects allows the opportunity to find potentially better biological control agents. therefore, in this study, bacteria from summer chafer (amphimallon solstitiale l., coleoptera: scarabaeidae) we isolated and identified the insecticidal effects of bacteria isolated from a. solstitiale and melolontha melolontha l. (common cockchafer, coleoptera: scarabaeidae) and the mixtures of these bacterial isolates were investigated on a. solstitiale larvae. crystals from ...	2005	16273040
top-down proteomics for rapid identification of intact microorganisms.	we apply maldi-tof/tof mass spectrometry for the rapid and high-confidence identification of intact bacillus spore species. in this method, fragment ion spectra of whole (undigested) protein biomarkers are obtained without the need for biomarker prefractionation, digestion, separation, and cleanup. laser-induced dissociation (unimolecular decay) of higher mass (>5 kda) precursor ions in the first tof analyzer is followed by reacceleration and subsequent high-resolution mass analysis of the resul ...	2005	16285700
comparative studies on potential of consortium and constituent pure bacterial isolates to decolorize azo dyes.	the decolorization potential of the consortium hm-4 constituted by mixing four laboratory isolates identified as bacillus cereus (bn-7), pseudomonas putida (bn-4), pseudomonas fluorescens (bn-5) and stenotrophomonas acidaminiphila (bn-3) was compared with that of individual isolates. six different azo dyes viz., c.i. acid red 88 (ar-88), c.i. acid red 119 (ar-119), c.i. acid red 97 (ar-97), c.i. reactive red 120 (rr-120), c.i. acid blue 113 (ab-113) and c.i. acid brown 100 (ab-100) were used in ...	2005	16289280
ligand requirements for glms ribozyme self-cleavage.	natural rna catalysts (ribozymes) perform essential reactions in biological rna processing and protein synthesis, whereby catalysis is intrinsic to rna structure alone or in combination with metal ion cofactors. the recently discovered glms ribozyme is unique in that it functions as a glucosamine-6-phosphate (glcn6p)-dependent catalyst believed to enable "riboswitch" regulation of amino-sugar biosynthesis in certain prokaryotes. however, it is unclear whether glcn6p functions as an effector or c ...	2005	16298301
use of oligonucleotide array for identification of six foodborne pathogens and pseudomonas aeruginosa grown on selective media.	identification of presumptive foodborne pathogens grown on selective media may take one to several days and requires a different battery of biochemical tests for each microorganism. a molecular identification method was developed in which universal primers were used to amplify the 16s to 23s rdna intergenic spacer of target microorganisms, and pcr products were hybridized to a panel of species-specific oligonucleotides that were immobilized on a nylon membrane. the seven target microorganisms we ...	2005	16300063
thermal resistance of spores from virulent strains of bacillus anthracis and potential surrogates.	the objective of this study was to determine the thermal resistance of spores of bacillus anthracis and potential surrogates. the heat resistance of spores suspended in buffer (ph 7.0 or 4.5), milk, or orange juice was determined at 70, 80, and 90 degrees c. d-values for b. anthracis strains sterne, vollum, and pasteur ranged from < 1 min at 90 degrees c to approximately 200 min at 70 degrees c and were lower under acidic than under neutral conditions. the d-values for b. anthracis spores fell w ...	2005	16300074
differential activity of a lectin from solieria filiformis against human pathogenic bacteria.	a lectin isolated from the red alga solieria filiformis was evaluated for its effect on the growth of 8 gram-negative and 3 gram-positive bacteria cultivated in liquid medium (three independent experiments/bacterium). the lectin (500 microg/ml) stimulated the growth of the gram-positive species bacillus cereus and inhibited the growth of the gram-negative species serratia marcescens, salmonella typhi, klebsiella pneumoniae, enterobacter aerogenes, proteus sp, and pseudomonas aeruginosa at 1000 m ...	2005	16302091
deposition of ctab-terminated nanorods on bacteria to form highly conducting hybrid systems.	gold nanorods and nanospheres capped with positively charged ctab are deposited on the surface of gram-positive bacterium having negatively charged teichoic acid brushes. the deposition rate is more than an order of magnitude faster than that for peptide and nucleic acid capped nanoparticles. for the nanorods, never been reported before, the strong electrostatic attraction causes the rods to bend conformally over the curved bacterium surface in random orientations. this leads to formation of an ...	2005	16351078
biofilm formation and sporulation by bacillus cereus on a stainless steel surface and subsequent resistance of vegetative cells and spores to chlorine, chlorine dioxide, and a peroxyacetic acid-based sanitizer.	biofilm formation by bacillus cereus 038-2 on stainless steel coupons, sporulation in the biofilm as affected by nutrient availability, temperature, and relative humidity, and the resistance of vegetative cells and spores in biofilm to sanitizers were investigated. total counts in biofilm formed on coupons immersed in tryptic soy broth (tsb) at 12 and 22 degrees c consisted of 99.94% of vegetative cells and 0.06% of spores. coupons on which biofilm had formed were immersed in tsb or exposed to a ...	2005	16355833
determination of the antimicrobial properties of oligo-2-hydroxy-1-naphthaldehyde.	oligo-2-hydroxy-1-naphthaldehyde (ohna) was synthesized by oxidative polycondensation using h2o2 (35%, aqueous solution), air o2 and naocl (34%, aqueous solution) by kaya and senol and the products were characterized by spectral techniques. antimicrobial activities of the first and second fractions of ohna were tested against corynobacterium xerosis ccm 2824, proteus vulgaris atcc 6897, staphylococcus epidermidis nrrl b-4877, s. aureus atcc 6538, enterobacter aerogenes atcc 13048, salmonella thy ...	2005	16355978
antibacterial activity of coumarins.	the antibacterial activity of coumarin per se and other 45 coumarin derivatives was tested against strains of bacillus cereus mip 96016, escherichia coli atcc 25922, pseudomonas aeruginosa atcc 27853, and staphylococcus aureus atcc 25923. the inhibitory effects of coumarins were affected by their substitution patterns. osthenol (44) showed the most effective antibacterial activity against gram-positive bacteria with mic values ranging between 125 and 62.5 microg/ml. these results suggested that ...	2005	16320610
efficient growth inhibition of bacillus anthracis by knocking out the ribonucleotide reductase tyrosyl radical.	bacillus anthracis, the causative agent of anthrax, is a worldwide problem because of the need for effective treatment of respiratory infections shortly after exposure. one potential key enzyme of b. anthracis to be targeted by antiproliferative drugs is ribonucleotide reductase. it provides deoxyribonucleotides for dna synthesis needed for spore germination and growth of the pathogen. we have cloned, purified, and characterized the tyrosyl radical-carrying nrdf component of b. anthracis class i ...	2005	16322104
binding of harvested bacterial exopolymers to the surface of calcite.	biologically produced exopolysaccharides (eps) affect calcite dissolution and precipitation. in this study, natural alkaliphilic microbial isolates were collected from biofilms on historic limestone. the isolates were screened for their ability to produce significant quantities of eps in cultures. the most productive isolates were identified by 16s rrna sequence analysis as a close relative of bacillus cereus. eps with different chemical structures were harvested from the isolates. isothermal ti ...	2005	16323775
antioxidant and antibacterial activities of rumex japonicus houtt. aerial parts.	we evaluated total phenolic content, antioxidant activity, reducing power and antibacterial activity of ethanol, hexane, chloroform, ethyl acetate and aqueous extracts of aerial parts of rumex japonicus houtt. the ethyl acetate extract had the highest amount of phenolic compounds. it also exhibited the highest reducing power and antioxidant activity when assayed by the 1,1-diphenyl-2-picrylhydrazyl (dpph), beta-carotene bleaching and superoxide radical methods. the ethyl acetate extract possesse ...	2005	16327154
spatial variation in bacillus thuringiensis/cereus populations within the phyllosphere of broad-leaved dock (rumex obtusifolius) and surrounding habitats.	the aim of this study was to determine the prevalence and toxin gene diversity of bacillus thuringiensis/b. cereus in the phyllosphere of broad-leaved dock (rumex obtusifolius) at a small spatial scale. b. thuringiensis/cereus populations were isolated from the phyllosphere of dock and neighbouring grass and in neighbouring soil using commercially available selective media which avoided the disadvantageous heat-shock selection procedure. the maximum density of b. thuringiensis/cereus in the dock ...	2005	16332339
distribution of genes encoding putative virulence factors and fragment length polymorphisms in the vrra gene among brazilian isolates of bacillus cereus and bacillus thuringiensis.	one hundred twenty-one strains of the bacillus cereus complex, of which 80 were isolated from a variety of sources in brazil, were screened by pcr for the presence of sequences (bcet, hbla, nhebc, plc, sph, and vip3a) encoding putative virulence factors and for polymorphisms in variable-number tandem repeats (vntr), using a variable region of the vrra open reading frame as the target. amplicons were generated from isolates of b. cereus and bacillus thuringiensis for each of the sequences encodin ...	2005	16332792
production and characterization of antibodies against each of the three subunits of the bacillus cereus nonhemolytic enterotoxin complex.	the nonhemolytic enterotoxin (nhe) is one of the two three-component enterotoxins which are responsible for diarrheal food poisoning syndrome caused by bacillus cereus. to facilitate the detection of this toxin, consisting of the subunits nhea, nheb, and nhec, a complete set of high-affinity antibodies against each of the three components was established and characterized. a rabbit antiserum specific for the c-terminal part (15 amino acids) of nhec was produced using a respective synthetic pepti ...	2005	16332805
flha influences bacillus thuringiensis plcr-regulated gene transcription, protein production, and virulence.	bacillus thuringiensis and bacillus cereus are closely related. b. thuringiensis is well known for its entomopathogenic properties, principally due to the synthesis of plasmid-encoded crystal toxins. b. cereus appears to be an emerging opportunistic human pathogen. b. thuringiensis and b. cereus produce many putative virulence factors which are positively controlled by the pleiotropic transcriptional regulator plcr. the inactivation of plcr decreases but does not abolish virulence, indicating th ...	2005	16332888
rapid ped-2e9 cell-based cytotoxicity analysis and genotyping of bacillus species.	bacillus species causing food-borne disease produce multiple toxins eliciting gastroenteritis. toxin assays with mammalian cell cultures are reliable but may take 24 to 72 h to complete and also lack sensitivity. here, a sensitive and rapid assay was developed using a murine hybridoma ped-2e9 cell model. bacillus culture supernatants containing toxins were added to a ped-2e9 cell line and analyzed for cytotoxicity with an alkaline phosphatase release assay. most bacillus cereus strains produced ...	2005	16333068
in-vitro antibacterial and cytotoxic activity of cobalt (ii), copper (ii), nickel (ii) and zinc (ii) complexes of the antibiotic drug cephalothin (keflin).	keflin (kefl) interacts with co(ii), cu(ii), ni(ii) and zn(ii) metal ions leading to complexes of the type m(kefl)2cl2 and m(kefl)cl2, which have been characterized by physicochemical and spectroscopic methods. magnetic moment, ir, electronic spectral and elemental analyses data suggest that keflin behaves tridentately forming octahedral or trigonal bipyramidal complexes with the metal ions mentioned above. the new compounds have been screened in-vitro for antibacterial and cytotoxic activity ag ...	2005	16335054
[structural and functional aspects of bacterial membranes and liposomes interactions].	effect of exogenous lipids on the morphology and enzymatic activity of bacillus cereus b4368 membrane has been studied. specific character of different lipids action on the membrane enzyme activity was found. this peculiarity had been taken into account by using liposomes in biological systems.	2005	16335239
outbreak of cutaneous bacillus cereus infections among cadets in a university military program--georgia, august 2004.	although bacillus cereus is known mainly as an agent of food poisoning, other infections caused by this organism have been documented in immunocompromised patients, including sepsis, meningitis, pneumonia, and wound infections. certain populations are at increased risk for b. cereus infection, including cancer patients, neonates, intravenous drug users, and patients with a history of trauma, surgery, or catheterization. primary cutaneous disease attributed to b. cereus in immunocompetent persons ...	2005	16340940
proximity ligation assays with peptide conjugate 'burrs' for the sensitive detection of spores.	the proximity ligation assay (pla) has previously been used for the sensitive and specific detection of single proteins. in order to adapt pla methods for the detection of cell surfaces, we have generated multivalent peptide-oligonucleotide-phycoerythrin conjugates ('burrs') that can bind adjacent to one another on a cell surface and be ligated together to form unique amplicons. real-time pcr detection of burr ligation events specifically identified as few as 100 bacillus anthracis, 10 bacillus ...	2005	16237122
the autolytic phenotype of the bacillus cereus group.	to determine the autolytic phenotype of five species in the bacillus cereus group.	2005	16238737
isolation and identification of nitrogen-fixing bacilli from plant rhizospheres in beijing region.	to isolate and identify nitrogen-fixing bacilli from the plant rhizospheres in beijing region of china.	2005	16238759
impact of non typical food matrice and cell density on bacillus cereus emetic toxin production.		2005	16366265
[symptoms of food-borne diseases and gastroenteritis in kyushu, japan].	in this study we analyzed the symptoms of gastroenteritis or food-borne disease caused by the 10 most prevalent pathogens: norovirus, salmonella, vibrio parahaemolyticus, campylobacter jejuni, clostridium perfringens, shiga toxin-producing escherichia coli (stec), enterotoxigenic e. coli (etec), shigella sonnei/flexneri (shigella), staphylococcus aureus, and emetic-type bacillus cereus. the symptoms diarrhea, vomiting, fever, abdominal pain, and headache, and the incubation period in 646 cases i ...	2005	16366357
purification and characterization of bacillus cereus protease suitable for detergent industry.	an extracellular alkaline protease from an alkalophilic bacterium, bacillus cereus, was produced in a large amount by the method of extractive fermentation. the protease is thermostable, ph tolerant, and compatible with commercial laundry detergents. the protease purified and characterized in this study was found to be superior to endogenous protease already present in commercial laundry detergents. the enzyme was purified to homogeneity by ammonium sulfate precipitation, concentration by ultraf ...	2005	16377846
detection and identification of intestinal pathogenic bacteria by hybridization to oligonucleotide microarrays.	to detect the common intestinal pathogenic bacteria quickly and accurately.	2005	16437687
gibberellins-producing rhizobacteria increase endogenous gibberellins content and promote growth of red peppers.	the growth of red pepper plants was enhanced by treatment with the rhizobacterium, bacillus cereus mj-1. red pepper shoots showed a 1.38-fold increase in fresh weight (fw) and roots showed a 1.28-fold fw gain. this plant growth-promoting rhizobacterium (pgpr) has been reported to produce gibberellins (gas). other gas-producing rhizobacteria, bacillus macroides cj-29 and bacillus pumilus cj-69, also enhanced the fw of the plants. they were less effective than b. cereus mj-1, though. the endogenou ...	2005	16410767
isolation and characterization of mesophilic cellulose-degrading bacteria from flower stalks-vegetable waste co-composting system.	fifteen mesophilic bacteria with high c(x) cellulase activities were isolated and purified from a mixed-culture enriched from a flower stalks-vegetable waste co-composting system. a cmcase test showed that the enzyme activity of these isolates ranged from 7.9 to 28.0 u ml(-1). although filter paper degrading capability was low in single culture, significant synergetic cellulose degradation were detected in four groups of mixed cultures, their degradation rates were 23.5%, 26.3%, 19.4% and 24.5%, ...	2005	16474195
[application of the multiplex pcr and pcr-rflp method in the identification of the bacillus anthracis].	the aim of this study was to apply the multiplex pcr and pcr-rflp method for the identification of the b. anthracis strains and to distinguish those bacteria from other members of the bacillus cereus group. the multiplex pcr method enables to detect the virulence factors, i.e. the toxin and the capsule in b. anthracis strains. to do that, the authors have used 5 primer pairs specific for the fragments of lef, cya, pag genes which are present in the pxo1 plasmid and encode the toxin, the cap gene ...	2005	16494204
[laboratory-based evaluation of a selective x-sa agar medium supplemented with chromogenic substrate for staphylococcus aureus].	the newly developed culture medium, x-sa agar medium (nissui pharmaceuticals co., ltd., tokyo) selective for staphylococcus aureus was evaluated for its ability to detect clinical isolates of s. aureus. besides s. aureus, x-sa agar media allowed the growth of coagulase-negative staphylococci, bacillus cereus and some isolates of corynebacteria. however, those species were easily distinguishable from the blue and convex colonies of s. aureus. when compared to the traditional egg yolk mannitol sal ...	2005	16536059
crystallization and preliminary x-ray crystallographic analysis of peptide deformylase (pdf) from bacillus cereus in ligand-free and actinonin-bound forms.	in bacteria, protein expression initiates with an n-formyl group and this needs to be removed in order to ensure proper bacterial growth. these formylation and deformylation processes are unique to eubacteria; therefore, inhibition of these would provide a novel antibacterial therapy. deformylation is carried out by peptide deformylase (pdf). pdf from bacillus cereus, one of the major pathogenic bacteria, was cloned into expression plasmid pet-28a (novagen), overexpressed in escherichia coli bl2 ...	2005	16508119
response of performance characteristics and fecal consistency to long-lasting dietary supplementation with the probiotic strain bacillus cereus var. toyoi to sows and piglets.	as part of an interdisciplinary research project, we studied the performance response of sows and their litters to the probiotic strain bacillus cereus var. toyoi as well as feces consistency of piglets. gestating sows (n=26) were randomly allotted into two groups. the probiotic b. cereus var. toyoi was administered by dietary supplementation to one group of sows and their respective litters (probiotic group) whereas the second group (control group) received no probiotic supplementation. the dur ...	2005	16429826
prevalence and characterization of clostridium perfringens from spices in argentina.	spices can present high microbial counts and clostridium perfringens, bacillus cereus, salmonella and shigella, among others have been isolated from spices. c. perfringens is an important pathogen agent causing, among other diseases, enteritis in humans caused by c. perfringens enterotoxin (cpe) which causes human food poisoning and enterotoxemia in domestic animals. the aims of the present work were (i) to establish the hygienic sanitary quality of some spices in san luis, argentina; (ii) to de ...	2005	16701595
phospholipase c from two bacterial strains acts differently on pure phospholipids and membrane bound glycosylphosphatidylinositol (gpi) anchors.	phospholipase c (plc) was purified to homogeneity from the culture filtrate of bacillus cereus (65-fold, 540 u/mg protein) and b. thuringiensis (76-fold, 306 u/mg protein) by conventional techniques of enzyme purification. the purified enzymes have the molecular mass of 34 kda and 38 kda respectively, as determined by sds-page. both the plcs exhibited identical sensitivity to ph, temperature, cations, anions and inhibitors like glutathione and p-chloromercuribenzoate. plc-bc showed a preference ...	2005	23923568
validation of an overall model describing the effect of three environmental factors on the apparent d-value of bacillus cereus spores.	several factorial models extending the famous bigelow model to describe the influence of the heating and recovery ph and a(w) conditions on bacterial heat resistance have been developed. these models can be associated in an overall multifactorial model describing the influences of heating and recovery conditions on d values. for bacillus cereus strain adqp 407 the model parameters characterising the environmental factor influences (ph, temperature, a(w)) were evaluated. determination of bacteria ...	2005	15854707
[isolation and study of azobenzene converting soil bacteria].	heterotrophic bacteria were isolated from soil and glass slides and classified as bacillus cereus snk12, paenibacillus polymyxa snk2, azotobacter chroococcum ankii, and ochrobacterium intermedium anki. their cultures could degrade azobenzene under the conditions of co-metabolism. a rapid test for the ability of bacteria to convert azobenzenes is proposed.	2005	15859461
the linear double-stranded dna of phage bam35 enters lysogenic host cells, but the late phage functions are suppressed.	bam35, a temperate double-stranded dna bacteriophage with a 15-kb linear genome, infects gram-positive bacillus thuringiensis cells. bam35 morphology and genome organization resemble those of prd1, a lytic phage infecting gram-negative bacteria. bam35 and prd1 have an outer protein coat surrounding a membrane that encloses the viral dna. we used electrochemical methods to investigate physiological changes of the lysogenic and nonlysogenic hosts during bam35 dna entry and host cell lysis. during ...	2005	15866940
use of a quartz crystal microbalance to investigate the antiadhesive potential of n-acetyl-l-cysteine.	the reduction of bacterial biofilm formation on stainless steel surfaces by n-acetyl-l-cysteine (nac) is attributed to effects on bacterial growth and polysaccharide production, as well as an increase in the wettability of steel surfaces. in this report, we show that nac-coated stainless steel and polystyrene surfaces affect both the initial adhesion of bacillus cereus and bacillus subtilis and the viscoelastic properties of the interaction between the adhered bacteria and the surface. a quartz ...	2005	15870362
practical application of dynamic temperature profiles to estimate the parameters of the square root model.	optimal experimental design for parameter estimation (oed/pe) is a promising method to improve parameter estimation accuracy and minimise experimental effort in the field of predictive microbiology. in this paper, the oed/pe methodology was applied on two practical examples: the growth of bacillus cereus and enterobacter cloacae in liquid whole egg product. both strains were recovered from samples of a commercial product. the goal of the modelling exercise was to quantify the influence of temper ...	2005	15878409
premicellar complexes of sphingomyelinase mediate enzyme exchange for the stationary phase turnover.	during the steady state reaction progress in the scooting mode with highly processive turnover, bacillus cereus sphingomyelinase (smase) remains tightly bound to sphingomyelin (sm) vesicles (yu et al., biochim. biophys. acta 1583, 121-131, 2002). in this paper, we analyze the kinetics of smase-catalyzed hydrolysis of sm dispersed in diheptanoylphosphatidyl-choline (dc7pc) micelles. results show that the resulting decrease in the turnover processivity induces the stationary phase in the reaction ...	2005	15878423
[heat treatment for the control of bacillus cereus spores in foods].	the effect of heat treatments on food products related to soybean curd contaminated with bacillus cereus spores was investigated for the purpose of preventing food-poisoning outbreaks by b. cereus. in the case of b. cereus strains isolated from foods, heating foods inoculated with the spores for 20 min at 70 degrees c, 5 min at 75 degrees c, 2 min at 100 degrees c, or 10 sec with a microwave oven plus reheating after standing for 2 hr at 25 degrees c reduced the number of surviving cells within ...	2005	15881248
a ferric dicitrate uptake system is required for the full virulence of bacillus cereus.	bacillus cereus is an opportunistic human pathogen of increasing prevalence. analysis of the bacillus cereus genome sequence identified a potential ferric dicitrate uptake system. the three-gene operon was confirmed to be negatively regulated by the ferric uptake repressor (fur). the fec operon was genetically silenced using the integration suicide vector pmutin4. the mutant strain displayed no growth defect under iron-limited conditions but was unable to grow on ferric citrate as a sole iron so ...	2005	15886918
necrotizing infection due to bacillus cereus mimicking gas gangrene following penetrating trauma.	an 8-year-old boy presented with fulminant necrotizing infection resembling gas gangrene following penetrating trauma from a tree branch. bacillus cereus was isolated from tissue specimens, showing that unexpected pathogens can be isolated. it is essential to submit specimens for culture, as this organism is typically resistant to beta-lactam antibiotics and metronidazole, the empiric choice for gas gangrene.	2005	15891547
host-pathogen interactions: a proteomic view.	host-pathogen interactions reflect the balance of host defenses and pathogen virulence mechanisms. advances in proteomic technologies now afford opportunities to compare protein content between complex biologic systems ranging from cells to animals and clinical samples. thus, it is now possible to characterize host-pathogen interactions from a global proteomic view. most reports to date focus on cataloging protein content of pathogens and identifying virulence-associated proteins or proteomic al ...	2005	15892564
the exsa protein of bacillus cereus is required for assembly of coat and exosporium onto the spore surface.	the outermost layer of spores of the bacillus cereus family is a loose structure known as the exosporium. spores of a library of tn917-ltv1 transposon insertion mutants of b. cereus atcc 10876 were partitioned into hexadecane; a less hydrophobic mutant that was isolated contained an insertion in the exsa promoter region. exsa is the equivalent of safa (yrba) of bacillus subtilis, which is also implicated in spore coat assembly; the gene organizations around both are identical, and both proteins ...	2005	15901704
successful treatment of bacillus cereus meningitis following allogenic stem cell transplantation.	we report the case of a 19-yr-old boy, who received an allogeneic stem cell transplantation for the second relapse of hodgkin's disease. the patient developed seizures and flaccid hemiparesis on day +10. meningoencephalitis induced by bacillus cereus was diagnosed. the treatment consisted of appropriate antibiotics, g-csf and removal of the central venous line. infection control and nearly full neurological recovery was achieved. immunocompromised patients susceptible to b. cereus infection, ind ...	2005	15910391
bacterial strains from moldy buildings are highly potent inducers of inflammatory and cytotoxic effects.	we aimed to identify inflammatory and cytotoxic potential of individual indoor air bacterial and fungal strains, as well as extracts of indoor air filter samples containing bacteria and fungi. mouse raw264.7 macrophages were exposed in vitro to four bacterial strains; streptomyces californicus, mycobacterium terrae, bacillus cereus and pseudomonas fluorescens, and three fungal strains; penicillium spinulosum, aspergillus versicolor and stachybotrys chartarum. furthermore, raw264.7 macrophages we ...	2005	15910531
dynamic kinetic resolution of amino acid amide catalyzed by d-aminopeptidase and alpha-amino-epsilon-caprolactam racemase.	amino acid amide racemizing activity was discovered in alpha-amino-epsilon-caprolactam (acl) racemase (ec 5. 1. 1. 15) from achromobacter obae. the enzymatic synthesis of d-alanine from l-alanine amide has been demonstrated by use of d-aminopeptidase (dap; ec 3. 4. 11. 19) from ochrobactrum anthropi c1-38 and acl racemase. the conversion of 45 mm l-alanine amide was carried out at 30 degrees c for 7 h; l-alanine amide was completely converted to d-alanine, and no l-alanine was detected. the resu ...	2005	15913357
partial purification and characterization of protease enzyme from bacillus subtilis and bacillus cereus.	the aim of this experimental study was to isolate and partially purify protease enzyme from bacillus cereus and bacillus subtilis. protease enzyme is obtained by inducing spore genesis of bacteria from bacillus species in suitable nutrient plates. the partial purification was realized by applying, respectively, ammonium sulfate precipitation, dialysis, and deae-cellulose ion-exchange chromatography to the supernatant that was produced later. optimum ph, optimum temperature, ph stability, and tem ...	2005	15917598
microbial synthesis and characterization of physiochemical properties of polyhydroxyalkanoates (phas) produced by bacteria isolated from activated sludge obtained from the municipal wastewater works in hong kong.	the first objective of this study was the measurement of physical properties of p(3hb-co-3hv) copolymers with different (hydroxybutyrate) hb to (hydroxyvalerate) hv ratios produced by bacillus cereus (try2) isolated from activated sludge. the 3hv phbv copolymers were 0.05, 22.6, 39.2, 54.1, and 69.1 mol%, respectively. the second objective was to study possible waste water treatment and production of phas at the same time by b. cereus (try2) and pseudomonas spp. (tob17) (both were isolated from ...	2005	15920276
hydrocarbon biodegradation in oxygen-limited sequential batch reactors by consortium from weathered, oil-contaminated soil.	we studied the use of sequential batch reactors under oxygen limitation to improve and maintain consortium ability to biodegrade hydrocarbons. air-agitated tubular reactors (2.5 l) were operated for 20 sequential 21-day cycles. maya crude oil-paraffin mixture (13,000 mg/l) was used as the sole carbon source. the reactors were inoculated with a consortium from the rhizosphere of cyperus laxus, a native plant that grows naturally in weathered, contaminated soil. oxygen limitation was induced in th ...	2005	15920621
formation of the spore clumps during heat treatment increases the heat resistance of bacterial spores.	effects of the clumping of bacterial spores on their heat resistance as a result of heat treatment were investigated. spore suspensions of bacillus cereus, bacillus coagulans and bacillus licheniformis were heated at 85 degrees c. survivor curves of the three strains showed tailing in all treatments after 30 min. as the treatment time increased, the formation of spore clumps increased in all strains after 20 min. relative hydrophobicity of the spore surface increased as a result of heat treatmen ...	2005	15925006
chromium-resistant bacteria and cyanobacteria: impact on cr(vi) reduction potential and plant growth.	two chromium-resistant bacterial strains, bacillus cereus s-6 and ochrobactrum intermedium crt-1, and two cyanobacterial strains, oscillatoria sp. and synechocystis sp., were used in this study. at initial chromate concentrations of 300 and 600 microg k2cro4 ml(-1), and an inoculum size of 9.6 x 10(7) cells ml(-1), b. cereus s-6 completely reduced cr(vi), while o. intermedium crt-1 reduced cr(vi) by 98% and 70%, respectively after 96 h. at 100 microg k2cro4 ml(-1), synechocystis sp. mk(s) and os ...	2005	15926088
production of catechol-siderophore and utilization of transferrin-bound iron in bacillus cereus.	in the present study we attempted to ascertain whether bacillus cereus was able to produce catechol-siderophore(s), and whether it was able to utilize transferrin-bound iron. the growth of b. cereus was stimulated in proportion to the iron-saturation level of the transferrin, and catechol-siderophores were produced in inverse proportion to this level. b. cereus was proved to uptake iron from partially iron-saturated transferrin or holotransferrin, without destroying the transferrin by its protea ...	2005	15930764
characterization of chito-oligosaccharides prepared by chitosanolysis with the aid of papain and pronase, and their bactericidal action against bacillus cereus and escherichia coli.	papain (from papaya latex; ec 3.4.22.2) and pronase (from streptomyces griseus; ec 3.4.24.31) caused optimum depolymerization of chitosan at ph 3.5 and 37 degrees c, resulting in lmmc (low molecular mass chitosan) and chito-oligomeric-monomeric mixture. the yield of the latter was 14-16% and 14-19% respectively for papain- and pronase-catalysed reactions, depending on the reaction time (1-5 h). hplc revealed the presence of monomer(s) and oligomers of dp (degree of polymerization) 2-6, which was ...	2005	15932346
influence of glutamate on growth, sporulation, and spore properties of bacillus cereus atcc 14579 in defined medium.	a chemically defined medium in combination with an airlift fermentor system was used to study the growth and sporulation of bacillus cereus atcc 14579. the medium contained six amino acids and lactate as the main carbon sources. the amino acids were depleted during exponential growth, while lactate was metabolized mainly during stationary phase. two concentrations of glutamate were used: high (20 mm; ylhg) and low (2.5 mm; yllg). under both conditions, sporulation was complete and synchronous. s ...	2005	15933027
phosphatidylinositol-specific phospholipase c of bacillus anthracis down-modulates the immune response.	phosphatidylinositol-specific phospholipases (pi-plcs) are virulence factors produced by many pathogenic bacteria, including bacillus anthracis and listeria monocytogenes. bacillus pi-plc differs from listeria pi-plc in that it has strong activity for cleaving gpi-anchored proteins. treatment of murine dcs with bacillus, but not listeria, pi-plc inhibited dendritic cell (dc) activation by tlr ligands. infection of mice with listeria expressing b. anthracis pi-plc resulted in a reduced ag-specifi ...	2005	15944308
expanding the bactericidal action of the food color additive phloxine b to gram-negative bacteria.	phloxine b (d&c red no. 28) is a color additive for food, drugs, and cosmetics. it has been previously shown to have anti-staphylococcus aureus activities. in this work, the effect of phloxine b on various gram-negative bacteria and other gram-positive bacteria including bacillus cereus, bacillus thuringiensis, bacillus mycoides, bacillus subtilis, bacillus aureus, salmonella, escherichia coli and shigella was studied, along with the mechanism of anti-microbial activity. in the presence of fluor ...	2005	15949926
inhibitors of metallo-beta-lactamase generated from beta-lactam antibiotics.	the resistance of bacteria to the normally lethal action of beta-lactam antibiotics is largely due to the production of beta-lactamases that catalyze the hydrolysis of the beta-lactam. one class of these enzymes is a zinc-dependent metallo-beta-lactamase for which there are no clinically available inhibitors. the hydrolysis of cephalosporin beta-lactam antibiotics generates dihydrothiazines which subsequently undergo isomerization at c6 by c-s bond cleavage and through the intermediacy of a thio ...	2005	15952764
multilocus sequence typing reveals that bacillus cereus strains isolated from clinical infections have distinct phylogenetic origins.	eight strains of bacillus cereus isolated from bacteremia and soft tissue infections were assigned to seven sequence types (sts) by multilocus sequence typing (mlst). two strains from different locations had identical sts. the concatenated sequences of the seven sts were aligned with 65 concatenated sequences from reference sts and a neighbor-joining tree was constructed. two strains were distantly related to all reference sts. three strains were recovered in a clade that included bacillus anthr ...	2005	15796996
chitooligosaccharides--preparation with the aid of pectinase isozyme from aspergillus niger and their antibacterial activity.	an isozyme of pectinase from aspergillus niger with polygalacturonase activity caused chitosanolysis at ph 3.5, resulting in low-molecular weight chitosan (86%), chitooligosaccharides (cos, 4.8%) and monomers (2.2%). hplc showed the presence of cos with dp ranging from 2 to 6. charcoal-celite chromatography and re-n-acetylation of the cos followed by cd, ir, maldi-tof-ms and fab-ms analyses revealed an abundance of chitobiose, chitotriose and chitotetraose. the cos-monomeric mixture showed a bac ...	2005	15797142
activation of sphingomyelinase from bacillus cereus by zn2+ hitherto accepted as a strong inhibitor.	sphingomyelinase (smase) from bacillus cereus has been known to be activated by mg2+, mn2+, and co2+, but strongly inhibited by zn2+. in the present study, we investigated the effects of several kinds of metal ions on the catalytic activity of b. cereus smase, and found that the activity was inhibited by zn2+ at its higher concentrations or at higher ph values, but unexpectedly activated at lower zn2+ concentrations or at lower ph values. this result indicates that smase possesses at least two d ...	2005	15797235
"biocontrol of psychrotrophic enterotoxigenic bacillus cereus in a nonfat hard cheese by an enterococcal strain-producing enterocin as-48," a comment on: j. food prot. 67(7):1517-1521 (2004).		2005	15771164
bacillus cereus food poisoning and its toxins.	the genus bacillus includes members that demonstrate a wide range of diversity from physiology and ecological niche to dna sequence and gene regulation. the species of most interest tend to be known for their pathogenicity and are closely linked genetically. bacillus anthracis causes anthrax, and bacillus thuringiensis is widely used for its insecticidal properties but has also been associated with foodborne disease. bacillus cereus causes two types of food poisoning, the emetic and diarrheal sy ...	2005	15771198
effect of ph on the active site of an arg121cys mutant of the metallo-beta-lactamase from bacillus cereus: implications for the enzyme mechanism.	the zinc-dependent metallo-beta-lactamases are a group of bacterial enzymes that pose a threat to the future efficacy of present-day antibiotics. their mechanism is poorly understood, and there are no clinically useful inhibitors. while most members of the group contain two tightly bound zinc ions in their active sites, the bacillus cereus enzyme has a much lower affinity for its second zinc (zn2), thought to be due to the presence of arg121 immediately beneath the floor of the active site (cf. ...	2005	15779910
the antimicrobial activity of extracts of the lichen hypogymnia tubulosa and its 3-hydroxyphysodic acid constituent.	the antimicrobial activity and the mic values of the diethyl ether, acetone, chloroform, petroleum ether, and ethanol extracts of the lichen hypogymnia tubulosa and its 3-hydroxyphysodic acid constituent have been investigated against some microorganisms. at least one of the extracts or 3-hydroxyphysodic acid showed antimicrobial activity against aeromonas hydrophila, bacillus cereus, bacillus subtilis, escherichia coli, klebsiella pneumoniae, listeria monocytogenes, proteus vulgaris, salmonella ...	2005	15787241
eremophilane-type sesquiterpene derivatives from senecio aegyptius var. discoideus.	investigation of a ch(2)cl(2) extract of the aerial parts of senecio aegyptius var. discoideus afforded nine eremophilane compounds, of which six are new (1-6), namely, 1beta-hydroxy-8alphah-eremophil-7(11),9-dien-8beta,12-olide (1), 1beta,8alpha-dihydroxyeremophil-7(11),9-dien-8beta,12-olide (2), 1beta-hydroxy-8alpha-methoxyeremophil-7(11),9-dien-8beta,12-olide (3), 1-oxo-8alpha-methoxy-10alphah-eremophil-7(11)-en-8beta,12-lactam (4), 1beta,10beta-epoxy-8alpha-hydroxyeremophil-7(11)-en-8beta,12 ...	2005	15787455
identification of an antifungal chitinase from a potential biocontrol agent, bacillus cereus 28-9.	bacillus cereus 28-9 is a chitinolytic bacterium isolated from lily plant in taiwan. this bacterium exhibited biocontrol potential on botrytis leaf blight of lily as demonstrated by a detached leaf assay and dual culture assay. at least two chitinases (chicw and chich) were excreted by b. cereus 28-9. the chicw-encoding gene was cloned and moderately expressed in escherichia coli dh5alpha. near homogenous chicw was obtained from the periplasmic fraction of e. coli cells harboring chicw by a puri ...	2005	15715951
presence of bacillus cereus in street foods in gaborone, botswana.	the purpose of this study was to evaluate the microbiological safety and quality of street foods sold in gaborone, botswana. a total of 148 point-of-sale composite street food samples were bought and analyzed between june 2001 and may 2002. the analysis focused on the level of contamination of various street foods with bacillus cereus. the b. cereus (vegetative and spores), total spore, and total viable counts were determined on all the samples. also b. cereus isolates from 444 individual point- ...	2005	15726979
gil16, a new gram-positive tectiviral phage related to the bacillus thuringiensis gil01 and the bacillus cereus pbclin15 elements.	one of the most notable characteristics of tectiviridae resides in their double-layer coats: the double-stranded dna is located within a flexible lipoprotein vesicle covered by a rigid protein capsid. despite their apparent rarity, tectiviruses have an extremely wide distribution compared to other phage groups. members of this family have been found to infect gram-negative (prd1 and relatives) as well as gram-positive (bam35, gil01, ap50, and phins11) hosts. several reports have shown that tecti ...	2005	15743944
toxin-producing ability among bacillus spp. outside the bacillus cereus group.	a total of 333 bacillus spp. isolated from foods, water, and food plants were examined for the production of possible enterotoxins and emetic toxins using a cytotoxicity assay on vero cells, the boar spermatozoa motility assay, and a liquid chromatography-mass spectrometry method. eight strains produced detectable toxins; six strains were cytotoxic, three strains produced putative emetic toxins (different in size from cereulide), and one strain produced both cytotoxin(s) and putative emetic toxi ...	2005	15746316
fingerprinting of bacillus thuringiensis type strains and isolates by using bacillus cereus group-specific repetitive extragenic palindromic sequence-based pcr analysis.	a total of 119 bacillus thuringiensis strains (83 type strains and 26 native isolates), as well as five b. cereus group species, were analyzed by repetitive extragenic palindromic sequence-based pcr analysis (rep-pcr) fingerprinting. primers bc-rep-1 and bc-rep-2 were specifically designed according to an extragenic 26-bp repeated sequence found in the six b. cereus group genomes reported. a total of 47 polymorphic bands were detected, and the patterns varied from 5 to 13 bands in number and fro ...	2005	15746337
polysaccharide-producing bacteria isolated from paper machine slime deposits.	development of novel enzymatic methods for slime deposit control in paper mills requires knowledge of polysaccharide-producing organisms and the polysaccharide structures present in deposits. in this work, 27 polysaccharide-producing bacteria were isolated from slime samples collected from different parts of a paper machine. most of the isolates produced polysaccharides in liquid culture and nine of them were selected for production of polysaccharides for characterisation. the selected isolates ...	2005	15750806
gerr, a novel ger operon involved in l-alanine- and inosine-initiated germination of bacillus cereus atcc 14579.	bacillus cereus endospores germinate in response to particular nutrients. spores are able to sense these nutrients in the environment by receptors encoded by the gera family of operons. analysis of the bacillus cereus atcc 14579 genome revealed seven gera family homologues. using a transposon tn917-based insertional mutagenesis approach followed by an enrichment procedure to select for l-alanine-induced germination mutants, we isolated a mutant with a defect in the l-alanine germination pathway. ...	2005	15691930
behavior of escherichia coli cells and bacillus cereus spores on poplar wood crates by impedance measurements.	dry poplar wood crates and glass surfaces (bottoms of pyrex flasks) were contaminated with aqueous suspensions of escherichia coli collection de l'institut pasteur (cip) 54.8 cells or bacillus cereus cip 78.3 spores. after different periods of storage at 25 degrees c, the number of cells still present on both surfaces was determined by impedance microbiology. the physical and chemical properties of the wood greatly and rapidly decreased the number of cells. after 4 h of contact time, b. cereus r ...	2005	15693168
synthesis of schiff bases of 4-(4-aminophenyl)-morpholine as potential antimicrobial agents.	in the present study, a novel series of schiff bases of 4-(4-aminophenyl)-morpholine were synthesised and characterised by ir, 1h-nmr, 13c-nmr, mass spectral and elemental analysis. the compounds were screened for antibacterial (staphylococcus aureus (atcc 9144), staphylococcus epidermidis (atcc 155), bacillus cereus (atcc 11778), micrococcus luteus (atcc 4678), and escherichia coli (atcc 25922)) and antifungal (candida albicans (atcc 2091) and aspergillus niger (atcc 9029)) activities. the mini ...	2005	15694658