Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| veterinarian versus phone company. | 1991 | 245217 | |
| [plasmatic ampc and gmpc changes in asthmatic patients induced by beta stimulation or blockade and methylcholine (author's transl)]. | 1991 | 208056 | |
| the external doctorate. | 1991 | 258329 | |
| an investigation of occupational hand-arm vibration. | 1991 | 270380 | |
| [elementary resuscitation for nurses]. | 1991 | 258907 | |
| questions from an adn teacher: teaching basic nursing skills. | 1991 | 258016 | |
| on unprofessional conduct: court ruling sets precedent. | 1991 | 257908 | |
| pilot study on dental erosion in a norwegian electrolytic zinc factory. | in 12 workers in a norwegian factory using electrolytic methods to extract zinc, a relationship was found to exist between degree of erosion, number of teeth affected and the length of service. the prevalence of caries, abrasion and attrition was not higher than in comparable population groups. the correlation between diagnosis of erosion based on clinical examination, and diagnosis based on evaluation of color slides was good. | 1991 | 269772 |
| the poland anomalad: a clinical and cytogenetic study of seven cases. | seven patients with poland anomalad, 6 males and one female, are reported. four were affected on the right and 3 on the left side. the clinical, radiological and cytogenetic findings in these cases are discussed. in all the patients the nipples were asymmetric, the one on the affected side was sometimes set higher or lower than the one on the normal side, in relation to the extent of the muscle defect. one patient had coloboma of the iris. there was a greater variability of the defect of the upp ... | 1991 | 207076 |
| [listeriasis]. | 1991 | 259054 | |
| amputation: surgical technique and postoperative management. | the decision to amputate should be made with due consideration of the eventual rehabilitation of the patient. with this in mind, general principles of amputation surgery are outlined and specific levels of amputation are described. early post-amputation management has advanced over the last two decades to make rehabilitation goals the more readily reached. these advances are discussed. | 1991 | 269712 |
| implications of euthanasia--a nursing perspective. | 1992 | 265356 | |
| teaching the nursing process: the nurse educator. | 1992 | 257461 | |
| nursing care study: a patient with a fractured facial skeleton. | 1992 | 257440 | |
| involvement [editoral]. | 1992 | 265463 | |
| who bridges the gap? | 1992 | 257212 | |
| what future for the nhs? everyone agrees: there are far too many bureaucrats! | 1992 | 257590 | |
| [nursing department]. | 1992 | 257664 | |
| child care has changed in kasai mohalla. | 1992 | 257528 | |
| intensive care of the newborn--8. a baby with gastroschisis. | 1992 | 257519 | |
| renal cell carcinoma metastatic to the mandible. | 1992 | 270568 | |
| [a case of infant temporomandibular arthrosis (author's transl)]. | 1992 | 270532 | |
| cytoskeleton regulates beta-adrenergic hormonal stimulation in normal and leukemic white blood cells. | 1992 | 207582 | |
| practice commission completes its pilot project in quality assurance. | 1992 | 257313 | |
| the hypochondriac with the broken leg in bed one: a case for liaison psychiatric nursing. | 1992 | 257148 | |
| feature study tour index (conclusion). | 1992 | 257146 | |
| [one direction of pediatric nursing in the united states--pediatric nurse practitioners]. | 1992 | 257095 | |
| lomefloxacin versus trimethoprim/sulfamethoxazole in the treatment of adults with acute bacterial diarrhea. | this study compared the efficacy and safety of lomefloxacin with that of trimethoprim/sulfamethoxazole (tmp/smx) in the treatment of adult patients with acute diarrhea of bacterial etiology. two centers enrolled a total of 133 patients; 99 (74%) presented with severe symptoms. the patients were randomized to receive either lomefloxacin 400 mg once daily (n=68) or tmp/smx 160/800 mg twice daily (n=65) for five days. bacteriologic success was achieved in 89.5% of evaluation lomefloxacin-treated pa ... | 1992 | 18611521 |
| shigella flexneri bacteriuria in a saudi child. | 1992 | 17587005 | |
| characterization of zymomonas mobilis alkaline phosphatase activity in escherichia coli. | zymomonas mobilis phoa gene encoding alkaline phosphatase was expressed in escherichia coli cc118 carrying the recombinant plasmid pzap1. the ph optimum for this enzyme was 9.0 and showed a peak activity at 42 degrees c. this enzyme required zn2+ for its catalytic activity; however, mg2+ or ca2+ significantly affected the activity. this enzyme was found to be ethanolabile, and ethanol inhibition was reversed by addition of zn2+. kinetics of z. mobilis alkaline phosphatase production in e. coli c ... | 1992 | 1369189 |
| repeated, childhood vaginal bleeding is not always precocious puberty. | 1992 | 1728002 | |
| shigella flexneri enters human colonic caco-2 epithelial cells through the basolateral pole. | the commonly accepted view that enteroinvasive bacteria enter cells of the intestinal epithelial lining through the apical surface can be challenged in the case of shigellosis. this study is based on in vitro experiments that showed that the invasion of human colonic caco-2 cells by shigella flexneri occurred through the basolateral pole of these cells. in these experiments, the few bacteria that interacted with the apical surface either bound to microvilli of the cell dome without causing detec ... | 1992 | 1729185 |
| the agrobacterium tumefaciens vir gene transcriptional activator virg is transcriptionally induced by acid ph and other stress stimuli. | a set of agrobacterium tumefaciens operons required for pathogenesis is coordinately induced during plant infection by the vira and virg proteins. the intracellular concentration of virg increases in response to acidic media, and this response was proposed to be regulated at the level of transcription at a promoter (p2) that resembles the escherichia coli heat shock promoters. to test this hypothesis, we first constructed a virg-lacz transcriptional fusion. a strain containing this fusion had hi ... | 1992 | 1735712 |
| myosin-cross-reactive epitope of shigella flexneri invasion plasmid antigen b. | ipab, invasion plasmid antigen b, of shigella flexneri is a 62-kda protein required for invasion of intestinal epithelial cells. ipab is also one of several major protein antigens recognized by the humoral immune systems of most humans and monkeys after infection with shigellae. computer analysis of the deduced ipab amino acid sequence indicates that an alpha-helical structure is likely through much of the molecule. homology searches with protein data banks show that one alpha-helical domain bet ... | 1992 | 1370431 |
| biochemical and functional characterization of the leucocyte tyrosine phosphatase cd45 (cd45ro, 180 kd) from human neutrophils. in vivo upregulation of cd45ro plasma membrane expression on patients undergoing haemodialysis. | the biochemical and functional characterization, and the regulation of plasma membrane expression of the leucocyte tyrosine phosphatase cd45, have been investigated in neutrophils from healthy donors and patients undergoing haemodialysis. cd45 proteins of 180 kd and 130-150 kd were precipitated from neutrophils from both healthy subjects and haemodialysed patients. prolonged storing, as well as trypsin treatment of samples containing the 180-kd cd45 protein, generated the 130-150-kd polypeptides ... | 1992 | 1370931 |
| identification and characterization of b-cell epitopes of ipac, an invasion-associated protein of shigella flexneri. | invasion plasmid antigen c (ipac) is a 43-kda plasmid-encoded protein associated with the ability of shigellae to invade epithelial cells. this protein is consistently strongly recognized by sera from convalescent patients and monkeys experimentally infected with shigellae. the strong immunogenicity of ipac in the course of natural infection makes it a good candidate as a potentially protective antigen. to map the b-cell epitopes of this protein, the gene encoding ipac was cloned and expressed a ... | 1992 | 1373401 |
| immunochemical characterization of citrobacter strain pcm 1487 o-specific polysaccharide- and core oligosaccharide-protein conjugates. | o-specific-polysaccharide and core oligosaccharide were isolated from citrobacter strain pcm 1487 lipopolysaccharide and purified. the polysaccharide was selectively devoid of 4-deoxy-d-arabinohexose residues. covalent conjugates of the modified o-specific polysaccharide and of the core oligosaccharide with tetanus toxoid were prepared. immunochemical characterization of these conjugates proved that they are strong immunogens. using monospecific rabbit antisera raised against the conjugates, the ... | 1992 | 1376131 |
| outward currents in longitudinal colonic muscle cells contribute to spiking electrical behavior. | electrical events in longitudinal and circular muscles of the colon are different. longitudinal muscles generate action potentials superimposed upon small depolarizations termed myenteric potential oscillations and circular muscles generate slow wave events that persist for several seconds. differences between circular and longitudinal muscles may be related to the potassium channels these cells express. we have studied ca(2+)-dependent and voltage-dependent k currents of isolated longitudinal c ... | 1992 | 1378997 |
| the vascular endothelial growth factor proteins: identification of biologically relevant regions by neutralizing monoclonal antibodies. | angiogenesis plays critical roles in organ development during embryonic and fetal life, wound healing and in a variety of pathological conditions. vascular endothelial growth factor (vegf) is a secreted growth factor specific for vascular endothelial cells which induces angiogenesis in vivo. to gain a better understanding of the physiological role of vegf, we have generated and characterized four murine monoclonal antibodies (mabs) using the 165 amino acid species of recombinant human vegf as im ... | 1992 | 1380254 |
| induction of a humoral immune response to a shiga toxin b subunit epitope expressed as a chimeric lamb protein in a shigella flexneri live vaccine strain. | shigella flexneri vaccine strain (sfl124) given orally, evokes humoral immune response in human volunteers. such a strain, expressing antigenic epitope of b subunit of shiga toxin, would also provide immunity to the toxin produced by some species of shigella. a synthetic oligonucleotide, specifying an epitope [13-26 amino acids (aa)] of the b subunit of shiga toxin, was inserted into the lamb gene of escherichia coli and expressed in the s. flexneri vaccine strain. the chimeric lamb protein func ... | 1992 | 1381804 |
| a novel temporal expression pattern of three c/ebp family members in differentiating myelomonocytic cells. | members of the ccaat/enhancer binding protein (c/ebp) family have been shown to regulate the terminal differentiation of adipocytes and hepatocytes. in these cell lineages, high levels of c/ebp alpha are found only in mature, nondividing cells. using western blotting and immunohistochemical staining, we have determined the temporal order of expression for c/ebp alpha, c/ebp beta, and c/ebp delta in differentiating myelomonocytic marrow cells. these studies show a unique temporal pattern of c/ebp ... | 1992 | 1391942 |
| monitoring of antibiotic resistance in shigellae isolated in the netherlands 1984-1989. | during surveillance of antimicrobial resistance in shigella strains isolated in the netherlands from 1984 to 1989 and forwarded to the national institute of public health and environmental protection for typing, sensitivity to twelve antimicrobial agents was assessed. high rates of resistance to the older drugs of choice in treating shigellosis were found, i.e. ampicillin and trimethoprim-sulfamethoxazole. ampicillin resistance varied from 33 to 53% among shigella flexneri strains and from 10 to ... | 1992 | 1396730 |
| [changes in specific reaction of erythrocytes to the antigen after administration of methylene blue and sodium azide]. | 1992 | 1398187 | |
| unipolar reorganization of f-actin layer at bacterial division and bundling of actin filaments by plastin correlate with movement of shigella flexneri within hela cells. | shigella flexneri causes bacillary dysentery, an invasive disease of colonic epithelial cells in humans. the capacity of bacteria, once they have entered into a cell and escaped the phagocytic vacuole, to spread intracellularly and directly to adjacent cells without further extracellular passage is a key factor in invasion of the epithelial layer. movement of intracellular bacteria is dependent upon the polymerization of actin; concentration of the formed filaments to one end of the bacterium is ... | 1992 | 1398922 |
| specific lung mucosal and systemic immune responses after oral immunization of mice with salmonella typhimurium aroa, salmonella typhi ty21a, and invasive escherichia coli expressing recombinant pertussis toxin s1 subunit. | pertussis toxin (pt) is considered an essential protective component for incorporation into new generation vaccines against bordetella pertussis, the causative agent of whooping cough. traditionally, antipertussis vaccination has employed an intramuscular route. an alternative to this approach is to stimulate mucosal and systemic immune responses by oral immunization with live vaccine carrier strains of salmonella spp. or escherichia coli. recombinant s1 subunit of pertussis toxin was expressed ... | 1992 | 1398937 |
| vacb, a novel chromosomal gene required for expression of virulence genes on the large plasmid of shigella flexneri. | shigellae, the causative agents of bacillary dysentery, are capable of adhering to and invading epithelial cells and spreading into adjacent cells. a chromosomal mutant of shigella flexneri 2a ysh6000 with reduced invasive capacity was isolated by tn5 insertion mutagenesis. the linkage of the mutant phenotype to the tn5 insertion was determined by p1 phage transduction. the site of the tn5 insertion was assigned to a noti chromosomal restriction map, confirming that the virulence-associated locu ... | 1992 | 1400189 |
| molecular analysis of the glpfkx regions of escherichia coli and shigella flexneri. | we have identified a new gene, glpx, belonging to the glp regulon of escherichia coli, located directly downstream of the glpk gene. the transcription of glpx is inducible with glycerol and sn-glycerol-3-phosphate and is constitutive in a glpr mutant. glpx is the third gene in the glpfkx operon. the function of glpx remains unknown. glpx has an apparent molecular weight of 40,000 on sodium dodecyl sulfate-polyacrylamide gels. in addition to determining the e. coli glpx sequence, we also sequence ... | 1992 | 1400248 |
| functional roles assigned to the periplasmic, linker, and receiver domains of the agrobacterium tumefaciens vira protein. | vira and virg activate the agrobacterium tumefaciens vir regulon in response to phenolic compounds, monosaccharides, and acidity released from plant wound sites. vira contains an amino-terminal periplasmic domain and three cytoplasmic domains: a linker, a protein kinase, and a phosphoryl receiver. we constructed internal deletions of vira that truncate one or more domains and tested the ability of the resulting proteins to mediate environmentally responsive vir gene activation in vivo. the perip ... | 1992 | 1400253 |
| altered-function mutations of the transcriptional regulatory gene virg of agrobacterium tumefaciens. | three point mutations were isolated in the agrobacterium tumefaciens virg gene by screening for vir gene expression in the absence of added phenolic inducing compounds. all three mutations were localized in the predicted amino-terminal phosphoryl receiver domain of the protein. one mutant (n54d) bypasses the requirement for vira and phenolic inducers both for transcriptional activation of all tested vir promoters and for plant tumorigenesis. this mutant also activates vir gene expression efficie ... | 1992 | 1400254 |
| block of voltage-gated potassium currents by anticonvulsant u-54494a in mouse neuroblastoma cells. | u-54494a [(+-)-cis-3,4-dichloro-n-methyl-n-[2-(1- pyrrolidinyl)-cyclohexyl]-benzamide], an anticonvulsant and na channel blocker, was examined for its interaction with delayed rectifier k channels in mouse neuroblastoma cells (nie-115) using whole-cell and inside-out configurations of the patch clamp techniques. u-54494a at anticonvulsant doses reversibly blocked the tetraethylammonium-sensitive outward k current in a time-dependent manner without affecting the current-voltage relationship. anal ... | 1992 | 1403785 |
| temperature regulation of shigella virulence: identification of the repressor gene virr, an analogue of hns, and partial complementation by tyrosyl transfer rna (trna1(tyr)). | virr is the central regulatory locus required for coordinate temperature-regulated virulence gene expression in the human enteric pathogens of shigella species. detailed characterization of virr+ clones revealed that virr consisted of a 411 bp open reading frame (orf) that mapped to a chromosomally located 1.8kb ecori-acci dna fragment from shigella flexneri. insertional inactivation of the virr orf at a unique hpai restriction site resulted in a loss of virr+ activity. the virr orf nucleotide s ... | 1992 | 1406252 |
| identification and characterization of virk, a virulence-associated large plasmid gene essential for intercellular spreading of shigella flexneri. | seven virulence loci have been identified by tn5 insertion mutagenesis on the large 230 kb plasmid (pmysh6000) of shigella flexneri 2a. in this study, we used tn10 insertion mutagenesis and identified a novel virulence locus on pmysh6000 responsible for bacterial spread. characterization of the invading bacteria of the tn10 insertion mutants in the epithelial cells revealed that the bacteria were capable of at least some intracellular spreading but not intercellular spreading. immunoblot analysi ... | 1992 | 1406277 |
| c-receptor ligand blocks pulmonary clearance of atrial natriuretic peptide in isolated rat lungs. | pulmonary clearance of atrial natriuretic peptide (anp) was measured by indicator dilution technique in isolated perfused rat lungs with and without anp clearance receptor (c-receptor) blockade. approximately 50% of a bolus injection of 125i-anp was removed during a single pass through the lungs compared with the intravascular marker 14c-dextran. pulmonary clearance of 125i-anp was suppressed in a dose-dependent fashion by unlabeled anp. c-receptor blockade suppressed pulmonary clearance of 125i ... | 1992 | 1409730 |
| induction of pancreatic acinar pathology via inhalation of nicotine. | this study was conducted to determine the effects of nicotine inhalation on the onset, progression, and sequential development of pancreatic lesions. male sprague-dawley rats in groups of five were exposed to saline or nicotine aerosol twice daily for 15, 30, 45, and 60 min for 21 days. after sacrifice, blood samples were analyzed for plasma levels of nicotine, glucose, gastrin, and cholecystokinin. pancreatic tissues were examined for pathological lesions. while there were no significant differ ... | 1992 | 1409731 |
| elimination kinetics of volatile organics in humans using breath measurements. | during the past decade significant strides have been made toward understanding the sources and factors which lead to volatile organic chemical (voc) exposure in the general population. less is known, however, about the impact of low-level environmental exposure on human health. investigations are underway in a number of laboratories in an effort to determine the uptake, distribution, metabolism, and elimination kinetics for vocs in humans. we examined the elimination kinetics for the third phase ... | 1992 | 1422163 |
| a study of the 14 kda phospholipase a2 from rat liver using a continuous fluorescent displacement assay for the enzyme. | 1992 | 1426571 | |
| [intravascular autorosette-formation in hemolytic anemia]. | it has been established that monocyte and neutrophil autorosette-formation resultant in exocytic lysis of erythrocytes--constituents of autorosettes--takes a low-intensive course in the blood of children with chronic non-hematologic diseases. this process is significantly activated in hereditary microspherocytic and elliptocytic anemias. it has been suggested that autorosette-formation culminating in exocytic lysis of erythrocytes is a mechanism of eliminating pathological, damaged and old eryth ... | 1992 | 1426915 |
| purification, partial characterization, and heterologous radioimmunoassay of growth hormone (cgh) in red deer. | red deer growth hormone (cgh; 3.3 mg) was purified from an aqueous extract of seven pituitary glands (4.01 g wet weight) by preparative gel filtration on sephadex g-100, gel filtration on sephadex g-100 sf, and anion exchange chromatography on deae-sepharose cl-6b. purified cgh gave a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis with a molecular weight under reducing conditions of 20,000 da and gave a single peak on reverse-phase high-performance liquid chromatography ... | 1992 | 1426952 |
| candida albicans--saprophyte or pathogen? | skin scrapings taken from toe spaces of 200 healthy volunteers and from toe webs and groins of 150 pediatric patients were cultured for candida albicans using the serum germ-tube test. the results showed that candida albicans can be isolated in about 15% of normal toe spaces and 14% of children with normal groins. although candida albicans can be found in various grades of athlete's foot and also in some abnormal groins, we believe that it is not necessarily responsible for these conditions and ... | 1992 | 1428429 |
| [bacteriological examination of paper money]. | bacteriological survey of one hundred twenty currency notes was done. aerobic spore-forming bacilli (91%), staphylococcus epidermidis (63.3%), staphylococcus aureus (4.2%), enterococcus (24.1%), alpha-hem. streptococcus (4.1%), streptococcus pneumoniae (1.7%), corynebacterium (7.5%), lactobacilli (10.8%), klebsiella pneumoniae (31.7%), enterobacter (19.2%), e. coli (17.5%), proteus (1.7%), pseudomonas aeruginosa (0.8%), shigella flexneri (0.8%) were isolated from paper money samples. currency no ... | 1992 | 1435365 |
| genotypic and phenotypic characterization of an arod deletion-attenuated escherichia coli k12-shigella flexneri hybrid vaccine expressing s. flexneri 2a somatic antigen. | the construction and characterization of ecsf2a-2, an arod-deleted escherichia coli-shigella hybrid vaccine carrying chromosomal and plasmid genes from shigella flexneri and expressing s. flexneri 2a somatic antigen in association with e. coli k12 core are described. expression of hybrid lipopolysaccharide and deletion of arod resulted in the attenuation of phenotypic characteristics associated with pathogenicity. the addition of an arod deletion results in a requirement for an aromatic precurso ... | 1992 | 1441732 |
| [lipopolysaccharide changes in smooth-type avirulent shigella in comparison with initial virulent strains]. | the comparative study of the lipopolysaccharides (lps) of virulent and avirulent strains of s. sonnei, phase i (smooth colonies), has been made. electrophoresis of lps and subsequent densitometry of electrophoregrams have revealed the increase of the fraction of long 0-chains with a considerable number of recurring elements in 2 out of 3 lps preparations obtained from avirulent shigellae. in mice immunized with these lps preparations a considerably greater number of antibody-producing cells can ... | 1992 | 1441805 |
| concentration of milk secretory immunoglobulin a against shigella virulence plasmid-associated antigens as a predictor of symptom status in shigella-infected breast-fed infants. | we conducted a prospective, community-based study of healthy breast-fed mexican infants to determine the protective effects of anti-shigella secretory iga antibodies in milk. milk samples were collected monthly, and stool culture specimens were obtained weekly and at the time of episodes of diarrhea. nineteen breast-fed infants were found to have shigella flexneri, shigella boydii, or shigella sonnei in stool samples. ages of the 10 infants with symptomatic infection and the nine with asymptomat ... | 1992 | 1447644 |
| [characteristics of the dynamics of hematopoietic precursor cells cloned in diffusion chambers and recorded in experiments on mice and dogs irradiated in median lethal doses]. | in experiments with mice and dogs irradiated with ld50, it was shown the postirradiation depopulation of haemopoietic polypotent (cfus) cell-precursors in mouse bone marrow was more pronounced than that of granulocytic and macrophagal cells (cfudc). the rate of repopulation of cfus during the first week was higher than that of cfudc (t1/2 was 2.5 and 8.8 days respectively). in dogs, one could notice a partial change in the colony formation, a prolonged plateau period in the postirradiation cfudc ... | 1992 | 1448562 |
| [bacteremia caused by shigella flexneri]. | 1992 | 1450269 | |
| shigellosis in children: a prospective hospital based study. | from 1985 to 1988, fecal samples of 950 hospitalized children suffering from diarrhea or dysentery were screened for shigella species using standard methods. shigella species were isolated as sole pathogen from 192 (20.2%) cases and s. flexneri type 2 was the predominant serotype. shigella infection was prevalent throughout the year with high isolation rate during the summer and early monsoon months. shigella strains isolated during the period were resistant to most of the commonly used drugs fo ... | 1992 | 1452309 |
| pathogenesis of providencia alcalifaciens-induced diarrhea. | providencia alcalifaciens is a member of the family enterobacteriaceae. there are reports that p. alcalifaciens can cause diarrhea, but the mechanism(s) by which it causes diarrhea is known. we studied p. alcalifaciens isolated from a child and two adults with diarrhea for enteropathogenicity. the three isolates did not exhibit any characteristic adherence to cultured hep-2 cell monolayers, and they did not produce enterotoxins, cytotoxins, or keratoconjunctivitis in the sereny test. two isolate ... | 1992 | 1452332 |
| detection of shigella dysenteriae type 1 and shigella flexneri in feces by immunomagnetic isolation and polymerase chain reaction. | a combination of immunomagnetic separation (ims) and a polymerase chain reaction (pcr) procedure was used for direct isolation and identification of shigella dysenteriae type 1 and shigella flexneri from feces. immunomagnetic particles were coated with monoclonal antibody masfb, which is specific for a common epitope of the o polysaccharides of s. dysenteriae type 1 and s. flexneri. bacteria bound to the beads were boiled in water, and target dna was amplified with a primer pair specific for a g ... | 1992 | 1452650 |
| [active tuberculosis in children who received inh chemoprophylaxis]. | twelve children who developed active tuberculosis even after receiving isoniazid (inh) chemoprophylaxis were seen at tokyo metropolitan children's hospital from 1982 through 1991. all cases received inh more than 9 mg/kg/day, except for one case in which the amount of inh administered at the referring hospital was unknown and streptomycin was administered together with inh. the age of starting inh prophylaxis ranged from 2 months to 13 years, and the age at which clinical symptoms and/or laborat ... | 1992 | 1453566 |
| [the regulation of dna repair processes in mammalian cells. iii. the effect of epidermal growth factor on the postradiation recovery of the cell cycle in human a 431 cells and embryonic fibroblasts]. | recovery of the cell cycle in cells a 431 and in human embryo fibroblasts (efh) differs much. unlike efh, a 431 cells have: 1) synchronized exit of cells from g1 into s phase after 5 gr irradiation; 2) g2-block; 3) much less manifestation of these two phenomena in the presence of egf; 4) a lesser effectiveness of the repair of dna single-strand breaks. egf stimulation of the repair of radiation-induced dna lesions, ssb in particular, may be of great importance for the postirradiation cell cycle ... | 1992 | 1455555 |
| retinal and cortical activity in human subjects during color flicker fusion. | pattern electroretinograms (perg) and cortical visually evoked potentials (vep) were simultaneously recorded from 7 visually normal and 1 protanopic subjects. stimuli were color checkerboards (0.5 degrees check size), phase-reversing at 17 hz (i.e. 34 reversals/sec). using a stepwise sweep procedure, the luminance of the red (lambda peak = 550 nm) and green (lambda peak = 630 nm) checks varied in 11 steps in opposite directions from 0 to 30 cd/m2, embracing the subjective equiluminance point. fo ... | 1992 | 1455696 |
| [the immunocorrective therapy of patients with acute dysentery]. | 1992 | 1455821 | |
| [the excretion of shigella antigens in persons recovering from dysentery]. | shigella antigens can be detected in the excreta of convalescents after dysentery for a long time. most frequently these antigens occur in feces, less frequently in urine and rarely in saliva. according to indirect data, s. flexneri 1-6 antigens can be detected in excreta for a longer period after convalescence than s. sonnei antigens. when antigen indication is used for the diagnosis of dysentery and epidemiological analysis is carried out, one should bear in mind the length of the agent persis ... | 1992 | 1455962 |
| [the etiological structure of shigellosis in the former ussr--an indicator of the activity of the main routes of infection transmission]. | the data on the etiological structure of shigella infections in the ussr in 1988-1989 are presented. the study showed the dominating role of s. flexneri with s. sonnei also retaining great importance in shigella infections. the process of the liquidation of s. dysenteriae and s. boydii infections began in some large cities. the domination of dysentery caused by s. flexneri and a high typhoid rate, particularly in central asia, were due to poor water supply of the population. the spread of dysent ... | 1992 | 1455964 |
| [an increase in the immunogenicity of bacterial antigens under the influence of one of the derivatives of muramyl dipeptide]. | as revealed in animal experiments, glucosaminylmuramyl dipeptide (gmdp), the synthetic analog of muramyl dipeptide, when introduced intraperitoneally in a single injection or orally, exhibits adjuvant activity with respect to citrobacter 0-antigens, shigella flexneri and enhances the protective properties of dysentery and pertussis vaccines. the stimulating properties of gmdp depend on its dose, the route of its administration, the time elapsed after its administration, its ratio to the concomit ... | 1992 | 1455969 |
| [the clinical assessment of the immune status of patients with acute flexner shigellosis]. | a total of 192 patients with flexner's dysentery 1 have been examined. as a result, sharply pronounced unbalance of cell-mediated and humoral immunity factors has developed in the acute period of dysentery, reaching its maximal manifestations in severe course of the disease. in case of a tendency to a prolonged course of dysentery at the period of convalescence, essential shifts in immunological characteristics persist and the infective agent is repeatedly isolated. | 1992 | 1455972 |
| red blood cells as an antigen-delivery system. | the use of adjuvants is usually required to induce strong immunological responses to protein antigens. however, in many cases these adjuvants cannot be extensively applied in human and veterinary vaccinations because of associated inflammatory reactions or granuloma formation. we show here that protein antigens (bovine serum albumin, hog liver uricase, and yeast hexokinase), coupled to autologous red blood cells by way of a biotin-avidin-biotin bridge, elicit an immunological response in mice si ... | 1992 | 1457052 |
| loose stools in the early neonatal period. | during a two-year period, the various factors associated with loose stools in the early neonatal period were studied among hospital born babies. low birth weight babies had a lower incidence of non-infective loose stools when compared to neonates with a birth weight of more than 2500 g (p < 0.001). newborns delivered by cesarean section (p < 0.001) and those born to women with more than two children (p < 0.02) had a greater frequency of loose stools. initiation of supplementary feeding and admin ... | 1992 | 1459697 |
| [diagnosis of acute diarrheal infections]. | 1992 | 1460834 | |
| characteristics of polymorphism at a vntr locus 3' to the apolipoprotein b gene in five human populations. | we have analyzed the allele frequency distribution at the hypervariable locus 3' to the apolipoprotein b gene (apob 3' vntr) in five well-defined human populations (kacharis of northeast india, new guinea highlanders of papua new guinea, dogrib indians of canada, pehuenche indians of chile, and a relatively homogeneous caucasian population of northern german extraction) by using the pcr technique. a total of 12 segregating alleles were detected in the pooled sample of 319 individuals. a fairly c ... | 1992 | 1463014 |
| the gene for familial mediterranean fever in both armenians and non-ashkenazi jews is linked to the alpha-globin complex on 16p: evidence for locus homogeneity. | familial mediterranean fever (fmf) is a recurrent inflammatory disorder characterized by short episodes of fever, peritonitis, pleuritis, and arthritis. while fmf has been shown to be inherited in an autosomal recessive fashion in both non-ashkenazi jews and armenian families, clinical differences have raised the possibility of genetic heterogeneity. as its pathogenesis is unknown, mapping of the gene for fmf may provide the first objective method for early and accurate diagnosis of this disease ... | 1992 | 1463015 |
| comparative effects of thyroxine and/or retinoic acid treatment in vivo on growth hormone synthesis and release by pituitaries from thyroidectomized rats. | thyroid hormones and retinoic acid (ra) coregulate growth hormone (gh) synthesis and release from cultured pituitary tumor cells by interacting with nuclear receptors that activate gh gene transcription. whether these two compounds share overlapping gh regulatory activities in vivo is unclear. therefore we compared the effects of in vivo replacement therapy with thyroxine (t4) and/or retinoic acid (ra) on gh synthesis and release in pituitaries from hypothyroid rats. three weeks after thyroidect ... | 1992 | 1464411 |
| effects of 5-fluorouracil on hematopoietic stem cells in normal and irradiated mice. | the effects of 5-fluorouracil (5-fu) on hematopoietic stem cells (cfu-s) and nucleated cells in mouse femur and spleen were studied in normal and x-irradiated mice (ddy-slc male, 8-10 weeks old). changes in the number of circulating blood cells also were investigated in mice treated with 5-fu. a single dose of 5-fu (150 mg/kg) was injected i.p. the femoral cfu-s decreased after 5-fu treatment up to day 3 when it reached 2% of the control value. the cells then increased, reaching a maximum about ... | 1992 | 1464854 |
| [the theoretical and practical interpretations of the basic manifestations of the epidemic process in shigellosis]. | 1992 | 1466177 | |
| expression of active octameric chicken cardiac mitochondrial creatine kinase in escherichia coli. | sarcomeric mitochondrial creatine kinase (mib-ck) of chicken was expressed in escherichia coli as a soluble enzyme by using an inducible phage-t7 promoter. up to one third of the protein in e. coli extracts consisted of soluble recombinant mib-ck in an enzymically active form. approx. 20 mg of nearly-homogenous mib-ck was isolated in a two-step isolation procedure starting with 1 litre of isopropyl beta-d-thiogalactopyranoside-induced e. coli culture, whereas previous attempts to express other c ... | 1992 | 1471992 |
| determinants of pathogenicity in xanthomonas campestris pv. vesicatoria are related to proteins involved in secretion in bacterial pathogens of animals. | one of the model systems investigated for studying plant bacterial pathogenesis is xanthomonas campestris pv vesicatoria, the causal agent of bacterial spot disease of pepper and tomato. genes necessary for both basic pathogenicity and the induction of the hypersensitive response in resistant plants (hrp genes) were previously isolated from x. c. pv. vesicatoria and characterized genetically. as a first step toward functional analysis, part of the hrp gene cluster, making up several loci, was se ... | 1992 | 1472717 |
| antibody response to oral streptococci in behçet's disease. | the serum antibody titers against oral streptococci were studied by enzyme-linked immunosorbent assay (elisa) both in patients with behçet's disease (bd) and control groups. the patients with bd showed significantly higher antibody titers to s. sanguis strains 113-20, 114-23, and 118-1 which were isolated from patients with bd, in comparison with control groups. also, the reactions of high-titered sera to the crude cell wall and soluble (or membrane) fractions of the 113-20 strain were observed ... | 1992 | 1474932 |
| [bacillary dysentery--1990]. | 1992 | 1475396 | |
| [chemical and serological study of lipopolysaccharide isolated from shigella flexneri 88-893 possessing a new type-antigen]. | the o-specific polysaccharide chain which represents a new type-antigen in lipopolysaccharide (lps) of shigella flexneri 88-893 was investigated. the o-polysaccharide chain was found to be composed of repeating units comprising rhamnose, n-acetylglucosamine and glucose (3:1:2). in the passive hemolysis test, group-6 antiserum of s. flexneri exhibited a high hemolytic titer (50% hemolysis titer: 7,900) against sheep red blood cells (srbc) sensitized with intact 893 lps, but virtually no hemolytic ... | 1992 | 1484404 |
| the mode of action of cfad of escherichia coli and virf of shigella flexneri and other members of the arac family of positive regulators. | 1992 | 1484494 | |
| [rectal temperature-time of death nomogram: dependence of corrective factors of body weight in significant thermal insulating conditions]. | 98 test coolings were made under various cooling conditions (moving air, two types of both clothing and covering) on dummies of real masses of 1, 3.3, 9.9, 24.5 and 33.4 kg resp. which cool under standard conditions (unclothed, uncovered, still air) like human bodies of 14, 33, 41, 83 and 104 kg resp. the results provide evidence on a nonlinear dependence of corrective factors of body weight upon the body weight. the dynamics of the dependence increases with the thickness of thermic isolation. t ... | 1992 | 1489321 |
| avoidance of endotoxin-induced inflammation during studies of albumin clearance from caprine joints. | intra-articular injection of radiolabelled, commercial goat serum albumin (gsa) produces acute arthritis in caprine joints. this inflammation distorts clearance values and vitiates studies of normal lymphatic function. endotoxin, routinely found in commercial albumin preparations, appears to cause this local reaction. we describe a simple method for the preparation of low-endotoxin, radioiodinated serum albumin from aseptically collected serum. we have used this technique to prepare gsa for use ... | 1992 | 1489542 |
| characterization, specificity, and igv gene usage of anti-lymphocyte monoclonal antibodies from perinatal mice. | previous studies have shown that cd5+ b cells predominate during development of the immune system and frequently secrete self-reactive antibodies, some of which appear to influence the development of the adult b cell repertoire. in addition, we now show that a high frequency of perinatally derived antibodies react with lymphocytes. hybridomas derived from perinatal liver and splenic b cells and from spleens of adult balb/c and c57bl/6 mice were screened by immunofluorescence on thymocytes. anti- ... | 1992 | 1489727 |
| dna adduct measurements and tumor incidence during chronic carcinogen exposure in animal models: implications for dna adduct-based human cancer risk assessment. | 1992 | 1489923 | |
| icsb: a shigella flexneri virulence gene necessary for the lysis of protrusions during intercellular spread. | shigella flexneri causes bacillary dysentery by invading epithelial cells of the colonic mucosa. we have characterized the icsb gene which is located on the virulence plasmid pwr100. after inactivation of icsb, the mutant strain remained invasive, but formed abnormally small plaques on hela cell monolayers, colonized only the peripheral cells of caco-2 islets, and was unable to provoke a keratoconjunctivitis in guinea-pigs. examination of infected hela cells showed that the icsb mutant was able ... | 1992 | 1495389 |
| [territorial differences in the nature of the etiological structure of shigellosis--a pattern or chance?]. | 1992 | 1496882 | |
| presence of specific immunoglobulin a-secreting cells in peripheral blood after natural infection with shigella sonnei. | the appearance of antigen-specific immunoglobulin a (iga) antibody-secreting cells (ascs) following natural infection with shigella sonnei during a common-source outbreak caused by this organism was evaluated in a modified enzyme-linked immunosorbent assay (elispot). a mean iga asc value of 2,131.6/10(6) cells against homologous s. sonnei lipopolysaccharide (lps) was detected in blood samples obtained from patients with bacteriologically proven s. sonnei shigellosis 5 and 10 days after the onset ... | 1992 | 1500527 |
| antigenic homology of endotoxin with a coliform mastitis vaccine strain, escherichia coli o111:b4 (j5). | this study examined recognition of heterologous gram-negative endotoxin by antibodies recognizing common lipopolysaccharide core antigens. gram-negative endotoxins from 11 heterologous bacterial strains were tested for recognition by antibodies against common lipopolysaccharide core antigens. serum was harvested from a calf immunized with the rc mutant, escherichia coli o111:b4 (j5), and affinity purified against endotoxin derived from an ra mutant, salmonella typhimurium, producing an antibody ... | 1992 | 1500577 |
| molecular and cellular biology of shigella flexneri invasiveness: from cell assay systems to shigellosis. | 1992 | 1505204 | |
| [etiological structure of dysentery in the republic of armenia]. | in this work materials on the etiological structure of shigella infections at different territories of armenia are presented. four shigella species have been found to circulate in armenia. the dominating etiological agent is s. flexneri with s. sonnei also playing an important role. the serological picture of s. flexneri is characterized by the prevalence of subserovar 2a. | 1992 | 1509848 |