Publications

plasma methionine depletion and pharmacokinetic properties in mice of methionine γ-lyase from citrobacter freundii, clostridium tetani and clostridium sporogenes.	pk studies were carried out after a single i.v. administration of 500 and 1000 u/kg by measuring of mgl activity in plasma samples. l-methionine concentration was measured by mass spectrometry. after single i.v. injection of 500u/kg the circulating t1/2 of enzymes in mice varies from 73 to 123min. the auc0-tinf values determined for mgl 500u/kg from c. freundii, c. tetani and c. sporogenes are 8.21±0.28, 9.04±0.33 and 13.88±0.39u/(ml×h), respectively. comparison of pk parameters of three mgl sou ...	2017	28178629
global molecular epidemiology of imp-producing enterobacteriaceae.	international data on the molecular epidemiology of enterobacteriaceae with imp carbapenemases are lacking. we performed short-read (illumina) whole-genome sequencing on a global collection of 38 imp-producing clinical enterobacteriaceae (2008 to 2014). imp-producing enterobacteriaceae (7 varieties within 11 class 1 integrons) were mainly present in the south pacific and asia. specific blaimp-containing integrons (in809 with blaimp-4, in722 with blaimp-6, and in687 with blaimp-14) were circulati ...	2017	28167555
κ-carrageenan enhances lipopolysaccharide-induced interleukin-8 secretion by stimulating the bcl10-nf-κb pathway in ht-29 cells and aggravates c. freundii-induced inflammation in mice.	background. the dietary usage of carrageenan as common food additive has increased observably over the last 50 years. but there is substantial controversy about its safety. methods. we investigated whether the κ-carrageenan could enhance lipopolysaccharide-induced il-8 expression by studying its actions on the tlr4-nf-κb pathway. the aggravating effect of κ-carrageenan on citrobacter freundii dbs100-induced intestinal inflammation was also investigated in a mouse model. results. our data show th ...	2017	28163398
bacterial microcompartment-directed polyphosphate kinase promotes stable polyphosphate accumulation in e. coli.	processes for the biological removal of phosphate from wastewater rely on temporary manipulation of bacterial polyphosphate levels by phased environmental stimuli. in e. coli polyphosphate levels are controlled via the polyphosphate-synthesizing enzyme polyphosphate kinase (ppk1) and exopolyphosphatases (ppx and gppa), and are temporarily enhanced by ppk1 overexpression and reduced by ppx overexpression. we hypothesised that partitioning ppk1 from cytoplasmic exopolyphosphatases would increase a ...	2017	28105684
aerobic and anaerobic biosynthesis of nano-selenium for remediation of mercury contaminated soil.	selenium (se) nanoparticles are often synthesized by anaerobes. however, anaerobic bacteria cannot be directly applied for bioremediation of contaminated top soil which is generally aerobic. in this study, a selenite-reducing bacterium, citrobacter freundii y9, demonstrated high selenite reducing power and produced elemental nano-selenium nanoparticles (nano-se(0)) under both aerobic and anaerobic conditions. the biogenic nano-se(0) converted 45.8-57.1% and 39.1-48.6% of elemental mercury (hg(0) ...	2017	28011305
outbreak of klebsiella pneumoniae carbapenemase-producing citrobacter freundii at a tertiary acute care facility in miami, florida.	objective to describe the investigation and control of a rare cluster of klebsiella pneumoniae carbapenemase-producing citrobacter freundii in a hospital in southern florida. methods an epidemiologic investigation, review of infection prevention procedures, and molecular studies including whole genome sequencing were conducted. results an outbreak of k. pneumoniae carbapenemase-3-producing c. freundii was identified at a tertiary hospital in florida in 2014. of the 6 cases identified, 3 occurred ...	2017	27923418
isolation of a facultative anaerobic exoelectrogenic strain lz-1 and probing electron transfer mechanism in situ by linking uv/vis spectroscopy and electrochemistry.	a new facultative anaerobic exoelectrogenic strain lz-1, belonging to citrobacter freundii, has been isolated. this strain can produce current densities of 843.9 and 865.6μacm(-2) using citrate or acetate as carbon source in a three-electrode configuration. the electricity generation performance was also analyzed in a dual-chamber mfc system, reaching a maximum power density of 1233mwm(-2). in addition to acetate and citrate, other carbon sources such as pyruvate, formate, acetate, citrate and f ...	2017	27918963
genetically unrelated multidrug- and carbapenem-resistant citrobacter freundii detected in outpatients admitted to a portuguese hospital.	non-clonal, carbapenem- and multidrug-resistant citrobacter freundii isolates were collected from unrelated outpatients admitted to a portuguese hospital emergency department. one patient lived in a nursing home and was never hospitalised, whereas the other patient was repeatedly hospitalised in this hospital. the aim of this study was to unveil the molecular mechanisms associated with the carbapenem resistance of these isolates and to assess its potential dissemination.	2017	27915087
profile of citrobacter freundii st2, a multi-acyl-homoserine lactone producer associated with marine dinoflagellates.	marine algae provide a unique niche termed the phycosphere for microorganism inhabitation. the phycosphere environment is an important niche for mutualistic and competitive interactions between algae and bacteria. quorum sensing (qs) serves as a gene regulatory system in the microbial biosphere that allows bacteria to sense the population density with signaling molecules, such as acyl-homoserine lactone (ahl), and adapt their physiological activities to their surroundings. understanding the qs s ...	2017	27807655