Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| epstein-barr virus specific antibodies in patients with coeliac disease. | 1985 | 3005191 | |
| [human monoclonal antibodies against surface antigens of langerhans cells from lymphocytes of diabetics transformed by epstein-barr virus]. | human monoclonal antibody against islet cell surface antigens was generated from a pre-diabetic patient's peripheral blood lymphocytes transformed with epstein-barr virus. reactivity of these transformed lymphocytes was evaluated using indirect immunofluorescence on rat islet cell suspensions and frozen sections of human pancreas. several lymphoblastoid cell lines that react with islet cell surface were obtained. preliminary immunoblots with enriched rat islet cell membrane antigens suggest a re ... | 1985 | 3004677 |
| [laboratory results in the evaluation of anti htlv-iii in subjects at risk]. | 1985 | 3008301 | |
| [acute polyradiculoneuropathy of guillain-barré. critical review of potential etiopathogeneses]. | the authors go over the etiopathogenetic hypothesis suggested, in the last years, a propos of the guillain-barrè syndrome. they point out that about two/thirds of cases are preceded by infectious symptoms of the upper respiratory and alimentary tracts, with prevailing viral etiology. the nervous lesion, which characterize the histology of the illness, seems related to immunological change. in particular one must emphasize the possibility that the guillain-barrè syndrome is an autoimmune disorder ... | 1985 | 3008302 |
| cytomegalovirus (cmv) infections in renal transplant recipients. preliminary results of prophylaxis by an intramuscular human hyperimmune cmv igg. | infectious diseases are the most serious complications in immunosuppressed patients and the major cause of death in renal transplant recipients (23, 44). besides bacterial, fungal and protozoan infections, viruses of the human herpes group (herpes simplex virus, varicella-zoster virus, epstein-barr virus, cytomegalovirus) are known to be of major importance (21, 37). the most common and clinically relevant virus of this group is the cytomegalovirus (cmv). | 1985 | 3008312 |
| cyclosporin a inhibits epstein-barr virus induced gamma-interferon production in vitro. | 1985 | 3008318 | |
| balanced terminal chromosome translocations develop in ebv-derived, but non-immortal cell lines from patients with mycosis fungoides. | five lymphoblastoid cell lines established from patients with mycosis fungoides were followed during long-term culture. they all expressed the epstein-barr virus nuclear antigen. although the established b-cell lines initially showed a normal karyotype, metaphases with balanced terminal translocations gradually occurred. these chromosome abnormalities occurred simultaneously with decreased cell proliferation leading in all cases to termination of the cell lines. this contrasts the usual finding ... | 1985 | 3010616 |
| [isolation of a new strain of eb virus with lytic and transforming properties]. | 1985 | 3011288 | |
| [burkitt's disease. apropos of apparently surgical forms]. | 1985 | 3013513 | |
| chemo-radiotherapy in nasopharyngeal carcinoma at ramathibodi hospital, bangkok. | combined chemotherapy (cis-platinum and 5 fu) and radiation therapy were given to 11 patients with stage iv (except 1) nasopharyngeal carcinoma. none had distant metastasis. mean duration of follow-up was 16.2 months. objective response (cr+pr) at the primary lesion were 10 out of 11 (90.9%), while cr was 7 out of 11 (63.6%). cr+pr at the regional node were 10 out of 10 (100%), while cr was 9 out of 10 (90%). there has been no recurrence so far. one patient died of hepatocellular carcinoma. side ... | 1985 | 3012790 |
| [etiopathogenesis of malignant tumors of the otorhinolaryngologic area]. | 1985 | 3014638 | |
| [enhanced transformation of human lymphocytes by chinese herbs]. | the enhancing effect of extracts from some chinese herbs on transformation of lymphocytes by epstein-barr (eb) virus was tested in soft agar system. daphne genkwa, wikstroemia chamaedaphne, wikstroemia indica, stellera chamaejasme and sparganium stoloniferum were found to activate early antigen (ea) of eb virus in raji cells and also enhance transformation of lymphocytes by eb virus. but some other herbs, such as abrus cantoniensis and bulbophyllum inconspicuum were not able to activate ea, henc ... | 1985 | 3015528 |
| mapping genetic elements of epstein-barr virus that facilitate extrachromosomal persistence of epstein-barr virus-derived plasmids in human cells. | the epstein-barr virus (ebv) genome becomes established as a multicopy plasmid in the nucleus of infected b lymphocytes. a cis-acting dna sequence previously described within the bamhi-c fragment of the ebv genome (j. yates, n. warren, d. reisman, and b. sugden, proc. natl. acad. sci. usa 81:3806-3810, 1984) allows stable extrachromosomal plasmid maintenance in latently infected cells, but not in ebv-negative cells. in agreement with the findings of yates et al., deletion analysis permitted the ... | 1985 | 3016506 |
| a putative origin of replication of plasmids derived from epstein-barr virus is composed of two cis-acting components. | a genetic element of epstein-barr virus, orip, when present on recombinant plasmids allows those plasmids to replicate and to be maintained in cells that express the epstein-barr virus-encoded nuclear antigen ebna-1. here we define the dna sequences required for orip activity. two noncontiguous regions of orip are required in cis for activity. one consists of approximately 20 tandem, imperfect copies of a 30-base-pair (bp) sequence. the other required region, approximately 1,000 bp away, is at m ... | 1985 | 3018528 |
| modulation of epstein-barr virus-induced b-cell activation by concanavalin a. | direct addition of the t-cell mitogen, concanavalin a (con a), to cultures of epstein-barr virus (ebv)-stimulated peripheral blood mononuclear cells (pbmc) resulted in a dose-dependent inhibition of immunoglobulin m (igm) secreted in the supernatant, as measured by an enzyme-linked immunosorbent assay. furthermore, con a inhibited igm secretion of isolated t-depleted cells stimulated with ebv, and both the proliferation and igm secretion of ebv-driven lymphoblastoid cell lines. t-enriched cells, ... | 1985 | 3017572 |
| epstein-barr virus as an etiological agent in the pathogenesis of lymphoproliferative and aproliferative diseases in immune deficient patients. | 1985 | 3017882 | |
| antibody response against early antigens in herpesviridae infections. | for the serological diagnosis of herpesvirus infection, increasing use is made of the determination of the antibodies against virus-specific early antigens. the presence of serum antibody to early antigens is a widely accepted marker of acute epstein-barr virus infection or varicella-zoster virus infection. controversial data are present in the literature about the significance of cytomegalovirus early antigens antibody response. this is discussed in view of the possibility that controversial re ... | 1985 | 3021515 |
| monoclonal antibody to the epstein-barr virus receptor induces human b lymphocyte activation and differentiation. | 1985 | 3022444 | |
| the frequency of non-a, non-b hepatitis in acute and chronic liver disease. | 1985 | 3025569 | |
| [cytotoxicity induced by the serum of patients with aids and at high risk of aids against cells infected with epstein-barr virus]. | serologic reactivity against ebv-infected lines was assayed in patients with aids (n = 4) or the aids related complex (aids-rc) (n = 46) residing in argentina. anti-vca serology was comparable to that of controls. however sera from aids and aids-rc could induce antibody dependent cell mediated cytotoxicity (adcc) to ebv-infected cell lines (p3hrik and raji). adcc activity could be recovered in the high molecular weight fractions of aids and aids-rc sera. adcc was observed in sera with high level ... | 1985 | 2829276 |
| isoprinosine enhances the activation of sensitized lymphocytes by epstein-barr virus antigens. | isoprinosine, a synthetic purine derivative and a potentially useful immunostimulating agent, was tested for its ability to enhance lymphocyte responses to epstein-barr virus (ebv) antigens and to autologous ebv-transformed lymphocytes. isoprinosine significantly enhanced the response of sensitized lymphocytes (i.e. from ebv-seropositive individuals) to ebv antigens, while it has no effect on the lack of response of unsensitized lymphocytes (i.e. from ebv-seronegative individuals) to these antig ... | 1985 | 2409036 |
| immunostimulation by cytomegalovirus (cmv): helper t cell-dependent activation of immunoglobulin production in vitro by lymphocytes from cmv-immune donors. | cytomegalovirus (cmv) is the cause of a number of different diseases ranging from self-limited benign infections in healthy adults to life threatening illnesses among immunocompromised hosts and newborns. suppression of cell-mediated immunity is often found in cases of acute cmv infection, and in addition, the virus may also be a potent stimulant of lymphoid cells in vivo. we studied cellular proliferation and immunoglobulin (ig) production induced by cmv to determine its effect on human lymphoc ... | 1985 | 2409150 |
| prognostic value of b-cell mitogen-induced and spontaneous thymidine uptake in vitro in chronic b-lymphocytic leukaemia cells. | blood lymphocytes from 50 patients with chronic b-lymphocytic leukaemia (b-cll) were cultured in vitro with and without the polyclonal b-cell activators (pba) dextran sulphate (dxs), lipopolysaccharide from e. coli (lps), and epstein barr virus (ebv). patients with blood lymphocytes that showed a high spontaneous or pba-induced 3h-thymidine uptake in 4 d cultures had a significantly shorter therapy-free survival than patients whose lymphocytes showed a low thymidine uptake. the dxs-induced cellu ... | 1985 | 2410008 |
| polymorphisms within the hla-drw6 haplotype. ii. protein charge heterogeneity reflects mlc subtyping of hla-drw6 homozygous cells. | we have analyzed the hla class ii gene products from hla-drw6 homozygous cells. epstein-barr virus-transformed b-cell lines were internally labeled with [35s]-methionine. an np-40 lysate of the cells was subjected to immunoprecipitation, first with a drw52-like-specific monoclonal antibody and subsequently with a dr-specific framework antibody. the dr region-encoded gene products were analyzed by one-dimensional gel isoelectric focusing and two-dimensional gel electrophoresis. it is shown that d ... | 1985 | 2410359 |
| identification and characterization of the epstein-barr virus receptor on human b lymphocytes and its relationship to the c3d complement receptor (cr2). | in pursuing studies on the early events in the infection of human b cells by epstein-barr virus (ebv), we examined the host cell attachment phase with a panel of b-cell-specific monoclonal antibodies. one of the monoclonal antibodies, okb7, directly blocked the attachment of purified ebv to b lymphocytes in the absence of a second anti-immunoglobulin antibody and thereby prevented ebv infection of tonsil and peripheral blood b cells. although earlier studies have shown a close association of the ... | 1985 | 2410629 |
| a novel leukemia t-cell line (pf-382) with phenotypic and functional features of suppressor lymphocytes. | a human leukemia t-cell line (pf-382) spontaneously derived from the pleural effusion of a child with t-cell acute lymphoblastic leukemia is described. the cell line, which has been maintained in culture for over 10 months, has a modal number of 46 chromosomes and is characterized by a chromosomal abnormality, present in most of the cells, consisting of a translocation between chromosome x and chromosome 15 (46x,xq-,15p+). the cells are not recognized by the okt3 and okt11 monoclonal antibodies ... | 1985 | 2410652 |
| purification to homogeneity and characterization of human b-cell differentiation factor (bcdf or bsfp-2). | human b-cell differentiation factor (bcdf) was purified to homogeneity by sequential filtration and chromatography of culture supernatants from tcl-na1 cells on an aca34 gel column and then on a mono p column with fast protein liquid chromatography and reversed-phase hplc. a 5300-fold enrichment in specific activity of bcdf with about 25% recovery was attained. the homogeneity of purified bcdf was evidenced by the following: (i) the specific activity was 1.7 x 10(7) units/mg of protein, (ii) onl ... | 1985 | 2410927 |
| a shared antigenic determinant between human b lymphocytes and carcinomas (blca). | we have raised a murine igm monoclonal antibody (ma6) against immunoprecipitate obtained by reacting the serum of an npc patient with extract of the burkitt lymphoma cell line, raji. it reacts against an antigen (blca) which is broadly represented on human b lymphoid tissues and cell lines but is different from the functional b-cell markers such as surface immunoglobulins, ia products, fc and complement receptors. blca was found to occur on lymphoid cell lines representing all stages of b-cell d ... | 1985 | 2411675 |
| carboxyl-terminal domain of the epstein-barr virus nuclear antigen is highly immunogenic in man. | the carboxyl-terminal one-third of the epstein-barr virus nuclear antigen (ebna-1) encoded by the bamhi restriction fragment k was synthesized in escherichia coli by use of a high-expression plasmid. the resultant 28-kda ebna fusion polypeptide, comprising 5-10% of the total soluble bacterial protein, was purified to apparent homogeneity by phosphocellulose and hydroxylapatite column chromatography. both rabbit monospecific antibodies and mouse monoclonal antibodies against 28-kda ebna gave nucl ... | 1985 | 2412231 |
| cytomegalovirus in the mononucleosis syndrome in children. | in 7 children aged 18 months to 7 years isolated from a group of 128 children with infectious mononucleosis the cytomegalovirus infection was found. infection was diagnosed by determination of antibodies against immediate early and late cmv antigen by means of the elisa test. besides that, antibodies were determined against the capsid antigen and early antigen of eb virus by the method of indirect immunofluorescence. in four children only cytomegalovirus infection was found and three had a mixed ... | 1985 | 2412401 |
| biotin-labeled dna probes for detection of epstein-barr virus by in-situ cytohybridization. | conventional laboratory diagnosis of ebv-related disease is now performed by one of three methods: serology, lymphocyte transformation assay, or epstein-barr nuclear antigen (ebna) staining of cell preparations. of these techniques, serology is the most widely used. however, this approach assumes an intact host immune system, which is absent or impaired in some of the more baffling ebv-related syndromes. detection of infectious virus by the lymphocyte transformation assay is labor-intensive, req ... | 1985 | 2412753 |
| effects of x-irradiation and sodium butyrate on cell-cycle traverse on normal and radiosensitive lymphoblastoid cells. | we have used a multi-parameter flow-cytometric technique to analyse changes in cell-cycle phase distribution (early and late g1, s and g2+m phases) for normal and x-ray-sensitive (ataxia-telangiectasia, a-t) lymphoblastoid cells exposed to x-irradiation and sodium butyrate (either alone or in combination). sodium butyrate, an inhibitor of histone deacetylase, is a useful pharmacological tool for determining the proposed role of a histone acetylation-based chromatin surveillance system in control ... | 1985 | 2412868 |
| monoclonal human anti-dna antibodies from eb virus-transformed lymphocytes of systemic lupus erythematosus (sle) patients. | sixteen monoclonal human anti-dna antibodies were obtained from epstein-barr virus-transformed lymphoblastoid cells of patients with systemic lupus erythematosus (sle) and were studied in terms of antigenic specificity. all of the antibodies showed polyspecificity to polynucleotides. among them, some antibodies had a specificity to single-stranded (ss) dna. especially, o-8 antibodies showed a preference for polynucleotides with pyrimidine bases. the binding specificity of the antibody was also s ... | 1985 | 2413066 |
| cdna of the immunoglobulin kappa chain of an epstein-barr virus-transformed human lymphoid cell line: partial sequence determination and bacterial expression. | we report the isolation, nucleotide sequence determination, and bacterial expression of a partial cdna for the immunoglobulin kappa chain from the epstein-barr virus-transformed human lymphoid cell line gm131. the cdna, cloned in pbr322 by use of oligo(dg) x oligo(dc) tails, yields two pst i fragments of 250 and 600 base pairs (bp). various restriction enzyme fragments of the cdna were subcloned in the vectors m13 mp10 and m13 mp11 for sequence analysis. as a result of instability of the 250-bp ... | 1985 | 2413453 |
| long-term culture and characterization of alloreactive t-cell infiltrates from renal needle biopsies. | long-term t-cell lines have been established by culturing renal needle biopsies from kidney recipients undergoing graft rejection. these cultures are maintained in the presence of interleukin 2, with weekly addition of irradiated donor lymphocytes as a source of antigen. the cells express t-cell markers, t3, t4, and t8, t-cell growth factor (il2) receptor, and hla-dr antigens. in one cell line, j2, the t4 and t8 antigens are found on two separate cell populations. this line is also shown to spec ... | 1985 | 2414252 |
| establishment and characterization of a new human eosinophilic leukemia cell line. | a human eosinophilic leukemia cell line, designated as eol, was established from the peripheral blood of a patient with philadelphia chromosome-negative eosinophilic leukemia (el). the eol cell line grows in single cell suspension with a doubling time of 48 hours for about one year. the reactivity of these cells was tested with a panel of monoclonal antibodies; they were found to express surface ia antigen, myeloid antigen (if10, my9) and membrane receptors for interleukin 2 (il-2, tac antigen). ... | 1985 | 2415185 |
| two monoclonal antibodies defining unique antigenic determinants on human b-lymphoma cells. | the non-hodgkin's lymphomas are a clinically, morphologically, and immunologically heterogeneous group of diseases. why lymphoma cells are unresponsive to normal regulatory growth controls and how they differ from normal lymphocytes are not well understood. in order to address these questions we have raised monoclonal antibodies to neoplastic b-cells. two of these, lm-26 and lm-155, show a high degree of specificity for b-cell lymphomas. when tested by fluorescence activated cell sorter analysis ... | 1985 | 2415242 |
| isolation and characterization of ni-specific t cell clones from patients with ni-contact dermatitis. | ni-specific t lymphocyte clones (tlc) were isolated from two patients with ni-contact dermatitis. all of the isolated tlc required both histocompatible antigen-presenting cells (apc) and ni for induction of proliferation. by using a panel of hla-typed epstein barr virus-transformed b cells (ebv-b cells) as apc and monoclonal anti-dr antibody, the clones were shown to recognize ni in the context of hla class ii determinants. all of the clones that were isolated are okt3+, okt4+, okt8-. in the pre ... | 1985 | 2415590 |
| production of monoclonal antibodies against prostatic acid phosphatase by in vitro immunization of human spleen cells. | monoclonal antibodies against human prostatic acid phosphatase (papase) were produced by immunization of human primary spleen cell cultures. dissociated spleen cells were cultured for 5-8 days in the presence of 100 ng/ml of papase and pokeweed mitogen (1:5000). following immunization, b cells were isolated and infected with epstein-barr virus (ebv). two weeks after ebv-transformation, cells were fused with either mouse myeloma cells (sp2/oag14) or human/mouse heteromyeloma cells (shm-d33). hybr ... | 1985 | 2415632 |
| biochemical characterization of two epstein-barr virus early antigen-associated phosphopolypeptides. | epstein-barr virus (ebv) nonproducer cells nc37 induced to viral early antigen (ea) synthesis by the tumor promotor 12-o-tetradecanoylphorbol-13-acetate (tpa) were labeled at day 4 after induction with 32p, and were analyzed by immunoprecipitation with human ea-positive sera. by employing this method the appearance of two phosphopolypeptides of 50 and 58k (pp50 and pp58) was well correlated with ea complex. partial v8 protease digestion and two-dimensional peptide analysis revealed that the poly ... | 1985 | 2416113 |
| molecular analysis of the rna and protein components recognized by anti-la(ss-b) autoantibodies. | the aim of this study was to determine whether sera with autoantibodies to the la(ss-b) nuclear antigen react with the same or different sets of cellular or viral ribonucleoproteins (rnps) and whether patients with anti-la(ss-b) comprised a homogeneous group with respect to phenotypic and serological markers. the 34 anti-la(ss-b) sera studied were detected in the course of screening 2,000 sera referred from patients with suspected or defined multisystem autoimmune disease. analysis of the molecu ... | 1985 | 2417765 |
| arrest of daudi cell growth by inactive influenza virus in-vitro. | we investigated the destructive capability of three influenza a strains, victoria, pr8, and their recombinant x47, against the human lymphoma cell line daudi. both victoria and x47 strains share the same envelope glycoproteins (h3n2), while pr8, the second parental strain of x47, differs in its envelope glycoproteins (hon1). the h3n2 strains and particularly the x47 recombinant were cytotoxic to daudi cells while the hon1 strain was not. to reduce the virulence of the oncolytic viruses, we inact ... | 1985 | 2419962 |
| interferon and 2-5a synthetase in patients with immune diseases. | 1985 | 2421319 | |
| evidence for active epstein-barr virus infection in patients with persistent, unexplained illnesses: elevated anti-early antigen antibodies. | forty-four patients, including 26 adults and 18 children under 15 years of age, were referred for evaluation of recurrent or persistent illnesses, with symptoms including pharyngitis, lymphadenopathy, fever, headaches, arthralgia, fatigue, depression, dyslogia, and myalgia. thirty-nine patients were positive for epstein-barr virus antibody with antibody levels compatible with active infection for at least 1 year. antiviral capsid antigen and anti-early antigen titers of patients were significant ... | 1985 | 2578266 |
| persisting illness and fatigue in adults with evidence of epstein-barr virus infection. | clinical, serologic, virologic, and immunologic evaluations for 31 adults with chronic illness and fatigue suggested that 23 had persisting epstein-barr virus infection. among these 23 patients, cellular immune mechanisms were generally normal, but 4 had mild immunoglobulin deficiencies. however, 20 patients had abnormal serologic profiles specific for epstein-barr virus shown by significantly elevated titers of antibodies to the viral capsid antigen or early antigen, or by a deficiency of late- ... | 1985 | 2578268 |
| amphotropic retrovirus vector transfer of the v-ras oncogene to human hematopoietic and stromal cells in continuous bone marrow cultures. | human continuous bone marrow cultures were established from intraoperative marrow specimens and infected with amphotropic murine leukemia virus (am-mulv) pseudotypes of kirsten or harvey murine sarcoma virus, and the biologic effects were compared with mouse continuous bone marrow cultures. cultures were tested for production of total nonadherent granulocytes and granulocyte-macrophage progenitor cells (gm-cfuc); virus replication by supernatant reverse transcriptase activity; percentage of adhe ... | 1985 | 2578839 |
| generation of epstein-barr virus antigen-specific suppressor t cells in vitro. | immunosuppression is a commonly observed phenomenon in epstein-barr virus (ebv)-associated disorders and malignancies. the purpose of this study was to determine whether ebv antigens could generate suppressor cell activity in vitro. peripheral blood lymphocytes (pbl) were first treated with various concentrations of ebv antigens or culture medium for 5 days and then with mitomycin c. the cells were then washed and tested for their ability to abrogate the blastogenic response of fresh, autologous ... | 1985 | 2579037 |
| b5, a new b cell-restricted activation antigen. | the characterization of a new human b cell-restricted activation antigen (b5) is described in this report. with the use of a monoclonal antibody to b5, we show that b5 can be detected on peripheral blood or splenic b cells after 1 day of stimulation with either anti-immunoglobulin, protein a, epstein barr virus, or pokeweed mitogen. in contrast, b5 was not expressed on resting b, t, or myeloid cells. more important, b5 could not be detected on activated t cells or monocytes. the b5 antigen was e ... | 1985 | 2579134 |
| large-scale purification of epstein-barr virus membrane antigen gp340 with a monoclonal antibody immunoabsorbent. | a purification method has been elaborated to isolate epstein-barr (eb) virus membrane antigen, gp340, in milligram amounts. the gp340 was prepared from detergent extracts of b95-8 cells by affinity chromatography with a monoclonal antibody immunoabsorbent. bound material was eluted and the eluate, consisting of 50% gp340, was then fractionated by gel filtration. the final gp340 product was antigenically active and 95% pure. the purification method was found to be rapid and reproducible with no l ... | 1985 | 2579161 |
| immune function in chronic active epstein-barr virus infection. | the spectrum of illness attributed to epstein-barr virus (ebv) includes patients with symptoms persisting for more than 1 year without any other obvious underlying disease. high titers of antibodies to ebv, either igg antiviral capsid antigen or anti-early antigen, can be demonstrated. in this study, 13 patients diagnosed as having chronic active ebv infection were examined to determine aspects of their immunologic status. morphological examination and fluorescent antibody analysis revealed no a ... | 1985 | 2579970 |
| human monoclonal antibodies produced by epstein-barr virus transformed cell lines bind protein a. | epstein-barr virus (ebv) is a polyclonal t-independent activator of viral receptor positive human b lymphocytes. lymphocytes infected in vitro with the virus are transformed into immortalized cell lines [nilsson, k, and klein, g. (1982) adv. cancer res. 37, 319]. in this way human cell lines that secrete specific igm, igg and iga monoclonal antibodies are established. protein a is also a polyclonal t-independent b cell activator [langone, j. j. (1982) adv. immunol. 32, 157], the targets of which ... | 1985 | 2580779 |
| allospecific t cell recognition of hla-a2 antigens: evidence for group-specific and subgroup-specific epitopes. | interleukin 2-dependent alloreactive cytotoxic t cell lines, with activity predominantly directed against the hla-a2 antigen, have been generated in vitro by stimulating blood mononuclear cells from donors nonimmune to the epstein-barr (eb) virus with appropriate numbers of eb virus-transformed b cells from a2-homozygous individuals. such effector cells were tested against a panel of eb virus-transformed target cell lines all expressing the serologically defined a2 antigen but typed into "common ... | 1985 | 2580783 |
| interferon induction in human leukocytes after in vitro exposure to cytomegalovirus or epstein-barr virus. | interferon (ifn) was produced after exposure of human mononuclear leukocytes and bone marrow cells to infectious or noninfectious cytomegalovirus (cmv) in vitro. the ifn was generated mainly by non-b lymphocytes. both alpha- and gamma-type ifn could be demonstrated. cmv antigens were usually not demonstrable in cmv-exposed leukocytes. addition of anti-ifn antibodies did not induce cmv antigens. thus, it seems that the endogenous production of ifn is not responsible for the difficulties in demons ... | 1985 | 2580812 |
| inhibition of epstein-barr virus (ebv) release from the p3hr-1 burkitt's lymphoma cell line by a monoclonal antibody against a 200,000 dalton ebv membrane antigen. | in raising murine hybridoma antibodies against epstein-barr virus (ebv)-induced membrane antigens (ma), we found one antibody that blocked the release of infectious ebv from cultured p3hr-1 cells. this monoclonal antibody (mab) recognized a 200 kd, phosphonoacetic acid-sensitive (late) ma, and did not directly neutralize virus without complement. when this mab was added to 33 degrees c-cultured, spontaneously ebv-producing p3hr-1 cells, the intracellular expression of viral capsid antigen and in ... | 1985 | 2580934 |
| effects of pure interferons on epstein-barr virus infection in vitro. | pure human gamma-interferon as well as alpha-interferon inhibited induction of immunoglobulin synthesis by epstein-barr virus but not by pokeweed mitogen in b lymphocytes from adult but not from newborn humans. the interferons inhibited the infected b lymphocytes directly, irrespective of the epstein-barr virus immune status of the donor, and their inhibitory effect was synergistic. | 1985 | 2580994 |
| production and characterization of a unique monoclonal antibody against human b cells (33.2.1). | a monoclonal antibody specific for a polymorphic antigen on human b cells (33.2.1) was produced and characterized. by flow cytometry, 33.2.1 was found to react with peripheral blood b cells, monocytes, and epstein-barr virus (ebv)-transformed b-cell lines, but not with peripheral blood t cells, mitogen-activated t cells, or allo- or autoactivated t cells. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) analysis showed that 33.2.1 recognizes a noncovalently bound bimolecular ... | 1985 | 2581708 |
| regulation of epstein-barr virus infection by recombinant interferons. selected sensitivity to interferon-gamma. | interferons (ifn) are antiviral proteins that may be important in mediating cellular defenses against epstein-barr virus (ebv) infection. however, the means by which ifn-alpha, -beta and -gamma modify ebv infectivity are not clear. we have evaluated the effects of purified recombinant preparations of all three classes of ifn on ebv-induced b lymphocyte proliferation and ig secretion. when added early after ebv infection, all three recombinant ifn reduced b cell outgrowth and ig secretion. ifn-ga ... | 1985 | 2581792 |
| use of novel chemical supplements in the establishment of three human malignant lymphoma cell lines (nu-dhl-1, nu-dul-1, and nu-amb-1) with chromosome 14 translocations. | three new cell lines have been established from patients with malignant lymphoma utilizing a human diploid feeder layer, pooled human serum, and the chemical supplements l-cysteine, iron-saturated transferrin, and bathocuproine disulfonate, a copper chelator. after a short period of growth, the 3 cell lines were successfully weaned from the feeder layers but continued to require human serum and the chemical supplements for up to 9 months of culture. the cell lines are currently grown in rpm1-164 ... | 1985 | 2581902 |
| cloned mouse ribonucleotide reductase subunit m1 cdna reveals amino acid sequence homology with escherichia coli and herpesvirus ribonucleotide reductases. | we have isolated and sequenced overlapping cdna clones containing the entire coding region of mouse ribonucleotide reductase subunit m1. the coding region comprises 2.4 kilobases and predicts a polypeptide of 792 amino acids (mr 90,234) which shows striking homology with ribonucleotide reductases from escherichia coli and the herpesviruses, epstein-barr virus and herpes simplex virus. the homologies reveal three domains: an n-terminal domain common to the mammalian and bacterial enzymes, a c-ter ... | 1985 | 2581962 |
| immunoregulation during acute infection with epstein-barr virus: dynamics of interferon and 2',5'-oligoadenylate synthetase activity. | immunoregulation during acute infection with epstein-barr virus (ebv) is incompletely understood, although species of interferon (ifn) may be important immunoregulatory molecules. ifn, 2',5'-oligoadenylate (2',5'-a) synthetase (an ifn-induced enzyme), t and natural killer cell subsets, and natural and anomalous killer cell functions were studied systemically during acute infectious mononucleosis. serum ifn was not detected (less than 1 international unit/ml) during infection. 2',5'-a synthetase ... | 1985 | 2582067 |
| identification of epstein-barr virus-induced polypeptides in p3hr-1 cells by protein immunoblot. | the protein immunoblot technique was used to identify epstein-barr virus-specific antigens present in sodium butyrate-induced p3hr-1 cells. using sera from patients with either nasopharyngeal carcinoma or arthritis, 16 polypeptides were detected ranging in molecular weight from 22k to 140k. each of the anti-ea-, anti-vca-positive sera were found to contain antibodies to different subsets of the antigens. a 72k protein was identified which was consistent with the nuclear antigen (ebna), and cultu ... | 1985 | 2582083 |
| [hepatitis non-a, non-b: epidemiologic, clinical, serologic and morphologic aspects]. | hepatitis non-a, non-b (hnanb) is due to one or more transmissible agents, probably viruses. epidemiologically, hnanb is transmitted predominantly by transfusion of blood or plasma derivatives, and percutaneous inoculation, but a non-percutaneous transmission by the fecal-oral route is also established. however, despite 10 years of intense world-wide research, the transmissible agent, or agents, have not been identified and there are no serological assays for either an antigen or an antibody tha ... | 1985 | 2582179 |
| human immune responses to synthetic peptides from the epstein-barr nuclear antigen. | humans infected with epstein-barr virus (ebv), the causative agent of infectious mononucleosis, develop antibodies against a nuclear antigen (ebna) that is present in virally transformed b lymphocytes. the ebna protein contains a unique glycine-alanine repeating sequence. we have synthesized peptides corresponding to various regions of the ebna molecule within and near this sequence. rabbit antibodies against the peptides within the sequence reacted directly with the ebna protein, as detected by ... | 1985 | 2981091 |
| persistent epstein-barr virus infection and a histiocytic sarcoma in a renal transplant recipient. | lymphomas occurring in renal transplant recipients are mostly large cell non-hodgkin's lymphomas (b-cell-derived). a sarcoma with all morphologic, immunohistochemical, and ultrastructural characteristics of a tumor of the mononuclear phagocytic system (mps) developed in a 23-year-old woman 1 year after renal transplantation. anti-epstein-barr-virus antibody titers proved to be exceptionally high, even in pretransplant sera. tumor-derived cells proved to be positive for epstein-barr nuclear antig ... | 1985 | 2981149 |
| epstein-barr virus infections in hairy cell leukemia patients in the presence of complement-dependent neutralizing antibody. | immune system status was characterized in patients with hairy cell leukemia (hcl) with respect to explaining their chronic or recurrent infections with epstein-barr virus. measures of cellular immune responsiveness for a group of 11 hcl patients were, in general, decreased when expressed as the proportion of tested patients with values less than 2 s.d. below mean values for a group of 17 healthy adults: t-cell enumeration, seven of 13; mitogen responsiveness of phytohemagglutinin, 10 of 11; conc ... | 1985 | 2981160 |
| successful immune reconstitution in severe combined immunodeficiency despite epstein-barr virus and cytomegalovirus infections. | cytomegalovirus (cmv) and epstein-barr virus (ebv), frequently found in the acquired immune deficiency syndrome (aids), have been suspected of contributing to the latter immunodeficiency. the ability of normal hla-identical sibling bone marrow to reconstitute an 8-month-old infant with severe combined immunodeficiency infected with these two viral agents is of interest. after presentation with severe mucocutaneous candidiasis, cavitary pulmonary disease, nodular cutaneous lesions, and hepatic ab ... | 1985 | 2981167 |
| biological activities of human recombinant interferon alpha/beta targeted by anti-epstein-barr virus monoclonal antibodies. | the requirement of high doses of interferon (ifn) during therapy severely restrict its application. thus a model using an epstein-barr virus (ebv) membrane antigen (ma) specific monoclonal antibody (mab) was developed to assess the feasibility of coupling minimal amounts of ifn to a mab and specifically delivering the ifn to the target cells. coupled ifn was first shown to retain fully both its anti-viral and anti-proliferative properties when tested on human tumor cell lines qimr-wil (ebv-ma+) ... | 1985 | 2981178 |
| insulin-stimulated receptor phosphorylation appears normal in cultured epstein-barr virus-transformed lymphocyte cell lines derived from patients with extreme insulin resistance. | insulin-stimulated phosphorylation of the insulin receptor was studied in cultured b-lymphocytes transformed by epstein-barr virus. in studies with cell lines derived from six normal subjects, insulin (10(-7) m) caused an average increase of approximately 200% in 32p incorporation into the 95k subunit of the insulin receptor. phosphorylation was rapid (detectable within 1-2 min) and reached a maximum level by 15 min. dose-response curves for receptor occupancy and phosphorylation were nearly sup ... | 1985 | 2981245 |
| characterization of a major protein with a molecular weight of 160,000 associated with the viral capsid of epstein-barr virus. | a monoclonal antibody designated v3 was produced against a late protein associated with the epstein-barr virus-induced viral capsid antigen complex. the antibody reacted with discrete patches in the nuclei of infected cells as well as with virus particles, as shown by immunofluorescence and ultrastructural immunoperoxidase staining. the molecular weight of the protein precipitated by this monoclonal antibody was ca. 160,000. all anti-viral capsid antigen antibody-positive sera tested in an enzym ... | 1985 | 2981328 |
| constitutive expression of epstein-barr virus-encoded rnas and nuclear antigen during latency and after induction of epstein-barr virus replication. | we examined the fate of two major products of latency as epstein-barr virus was induced to replicate. we studied a superinducible clone of hr-1 cells in the presence and absence of induction by phorbol ester, and we analyzed the x50-7 line with and without superinfection by an hr-1 viral variant which disrupts latency. the two methods of induction yielded qualitatively similar results. after induction, there was abundant synthesis of viral transcripts, amplification of viral dna, and the appeara ... | 1985 | 2981344 |
| epstein-barr virus transformation of human b lymphocytes despite inhibition of viral polymerase. | epstein-barr virus transformed human lymphocytes despite the presence of up to 500 microm acyclovir [9-(2-hydroxyethoxymethyl)guanine], a viral dna polymerase inhibitor. the transformed cells contained multiple epstein-barr virus genome copy numbers. functional viral dna polymerase is probably not required for cell transformation and the initial amplification of the viral genome. | 1985 | 2981351 |
| treatment of lymphoproliferation caused by epstein-barr virus. | 1985 | 2981411 | |
| lymphoma of host origin in a marrow transplant recipient in remission of acute myeloid leukemia and receiving cyclosporin a. | lymphomas are an uncommon complication of solid organ transplantation and rarely occur after marrow transplantation. when post-marrow transplant lymphomas have occurred, they have been of donor cell origin and when sought, epstein-barr virus dna has been found in the tumor. a 21-year-old woman developed a poorly differentiated lymphocytic lymphoma 6 months after bone marrow transplantation for acute myeloid leukemia in remission. cyclosporin a had been used as an immunosuppressant. a chromosomal ... | 1985 | 2981468 |
| small noncleaved b cell burkitt-like lymphoma with chromosome t(8;14) translocation and epstein-barr virus nuclear-associated antigen in a homosexual man with acquired immune deficiency syndrome. | this case report describes new manifestations of the acquired immune deficiency syndrome (aids) in a promiscuous homosexual man. investigation of upper gastrointestinal bleeding in the patient lead to discovery of a high-grade, small, noncleaved cell (burkitt-like) gastroduodenal lymphoma with visceral and extralymphatic extension. specific phenotyping of the lymphoma revealed that it was a monoclonal b cell lymphoma of mu kappa isotype. an in vitro cell line was established that was epstein-bar ... | 1985 | 2981469 |
| chronicity of epstein-barr virus infection. | 1985 | 2981494 | |
| thalassaemia and hodgkin lymphoma. | 1985 | 2981595 | |
| selective inhibitory effect of hu-ifn-gamma on the agarose clonability of tumor-derived lymphoid cell lines. | recombinant human interferon alpha (ifn-alpha) and interferon gamma (ifn-gamma) were compared for their ability to influence the proliferative capacity of tumor-derived cell lines and of normal b lymphocytes infected in vitro by epstein-barr virus (ebv). ebv-induced b-cell proliferation was suppressed almost completely when 10(2) u/ml ifn-alpha were added to the culture medium while the same dose of ifn-gamma had significantly lower inhibitory activity. the pure ifns differed in their ability to ... | 1985 | 2981638 |
| characterization of the hla-a2.2 subtype: t cell evidence for further heterogeneity. | five blood donors were identified whose hla-a2 is different from the common hla-a2. their a2 molecule (a2.2) had a more basic isoelectric point than normal a2 (a2.1). cytotoxic t lymphocytes (ctl) restricted by hla-a2.1, specific for influenza a and epstein-barr viruses, failed to lyse virus-infected target cells with hla-a2.2. identical patterns were obtained with both viruses. ctl from four of the a2.2-positive donors recognized target cells prepared from others in the group that shared only t ... | 1985 | 2981768 |
| establishment of the amsalem t-cell line from a patient with acute lymphoblastic leukemia. expression of e-receptor-associated antigens in cells incapable of forming e-rosettes. | a new t-cell line (amsalem) was established from the peripheral blood of a patient with pre-t leukemia. amsalem cells are unique in that they possess antigenic determinants associated with the e-receptor, yet fail to form rosettes with sheep red blood cells (srbc). amsalem cells were found to possess morphological and cytochemical features characteristic of t-lymphocytes, and were sensitive to the cytotoxic effect of rabbit antisera specific for t-cell antigens. in immunofluorescent tests with m ... | 1985 | 2981779 |
| a re-examination of the epstein-barr virus carrier state in healthy seropositive individuals. | a panel of 24 healthy seropositive donors have been followed prospectively over a period of 15 months and monitored (1) for the level of eb virus shedding in the throat by means of a sensitive cord-blood transformation assay; (2) for the level of virus-infected b cells in the blood via a new in vitro protocol where "spontaneous transformation" can be seen to titrate against input cell number; (3) for anti-eb viral antibody titres and (4) for the prevailing level of virus-specific memory t cells ... | 1985 | 2981780 |
| in vitro analysis of the epstein-barr virus: host balance in long-term renal allograft recipients. | four indices of the eb virus carrier state, for which quantitative in vitro assays now exist, have been monitored in 55 renal allograft recipients under long-term immunosuppression, each patient being tested on a single occasion. by comparison with parallel data from healthy control donors, the results indicate the extent to which virus replication in the throat and virus-infected b cells in the blood are increased as a result of immunosuppression; the concordance between these two independent i ... | 1985 | 2981781 |
| novel peripheral blood-derived human cell lines with properties of megakaryocytes. | for 18 mo, we derived 18 cell lines from 11 donors with various clinical profiles ranging from normal to leukemic. suspension cultures were initiated with 1 x 10(6) mononuclear blood cells/ml of nutrient medium containing 10% human serum and 10% lectin-stimulated human lymphocyte conditioned medium. the cultures were monitored weekly by morphological analyses of wright-giemsa-stained cell preparations. all successful cultures showed a significant decline in viability during the first 3-4 wk with ... | 1985 | 2981889 |
| antigen presentation by ebv-b cells to resting and activated t cells: role of interleukin 1. | we have previously demonstrated that epstein barr virus-transformed human b lymphocytes (ebv-b cells) present antigen to activated t cells (lines and clones) in a mhc-restricted manner. in the present study, using ebv-nonimmune donors, we demonstrate that ebv-b cells are unable to trigger tetanus toxoid (tt) antigen-specific proliferation in autologous highly purified resting t cells. ebv-b cells from these same individuals were able to present tt to autologous tt-specific activated t cell blast ... | 1985 | 2981918 |
| interleukin 2 receptors on human b cells. implications for the role of interleukin 2 in human b cell function. | in the present study, we examined the expression of interleukin 2 (il-2) receptors on normal human b cells as well as established b cell lines. anti-tac monoclonal antibody did not bind to freshly separated normal human b cells. unexpectedly, with the appropriate activation of the normal b cells by anti-mu antibody, phorbol myristate acetate, or staphylococcus aureus cowan i (sac), tac antigen was induced on the activated b cells. anti-tac antibody showed consistent reactivity with two b cell li ... | 1985 | 2981952 |
| interferon gamma production by herpes simplex virus antigen-specific t cell clones from patients with recurrent herpes labialis. | nineteen herpes simplex virus (hsv) antigen-specific human t lymphocyte clones were established from three volunteers with recent recurrent herpes labialis. all produced interferon gamma (ifn-gamma) at titres of 200 to 700 units/ml when cultured in vitro with hsv antigen and irradiated peripheral blood mononuclear cells (pbmc) as filler cells. all 10 of those clones whose phenotype was determined were leu 4+, leu 2-, leu 3+. interleukin 2 alone failed to induce ifn-gamma in titres greater than 1 ... | 1985 | 2981967 |
| the seroepidemiology of infection due to epstein-barr virus in southern india. | we investigated the seroepidemiology of infection due to epstein-barr virus (ebv) in 181 south indian subjects aged 0-25 years using the indirect immunofluorescence method to titrate antibodies to viral capsid antigen (vca), nuclear antigen (ebna), and early antigen (ea). the age-specific prevalence of igg antibodies to vca rose rapidly to 90% by the age of 5 years. the prevalence of vca-specific igm and the geometric mean titre of vca-specific igg antibodies were highest between the ages of 6 m ... | 1985 | 2981976 |
| two related but differentially expressed potential membrane proteins encoded by the ecori dhet region of epstein-barr virus b95-8. | three mrnas in the ecori dhet region of epstein-barr virus b95-8 were mapped. their 3' ends are coterminal. a latent gene containing three exons is transcribed from the ed-l1 promoter and was predicted to lead to expression of a 42-kilodalton protein. an unspliced late mrna is produced by transcription from the ed-l1a promoter within the first intron of the above gene and was predicted to lead to expression of a 28-kilodalton protein corresponding to the c-terminal two-thirds of the 42-kilodalto ... | 1985 | 2982035 |
| isolation of transforming and early antigen-inducing epstein-barr virus from nasopharyngeal carcinoma hybrid cells (npc-kt). | the biologic activities of epstein-barr (eb) virus from an epithelioid-nasopharyngeal carcinoma hybrid cell line (npc-kt) and three subclones from the npc-kt cells were examined. much infectious virus was released by treatment with 5-iodo-2'-deoxyuridine. all virus preparations from npc-kt cells and the subclones were found to possess both transforming and eb virus-induced early antigen (ea)-inducing activities. the lymphoblastoid cell lines, which were established by infection of human cord-blo ... | 1985 | 2982054 |
| new tools for the study of niemann-pick disease: analogues of natural substrate and epstein-barr virus-transformed lymphoid cell lines. | acid sphingomyelinase activity was determined in epstein-barr virus-transformed lymphoid cell lines (lcl) established from patients affected with niemann-pick disease (npd) using several substrates: sphingomyelin derivatives, radiolabeled [14c]sphingomyelin (sm), fluorescent n-(10-(1-pyrene)decanoyl)sphingomyelin (p10-sm) or colored trinitrophenylaminolauryl-sphingomyelin, and the chromogenic non-natural substrate 2-n-(hexadecanoyl)amino-4-nitrophenylphosphoryl-choline. lcl from npd type a and t ... | 1985 | 2982124 |
| immunoglobulin jh, c mu, and c gamma gene rearrangements in human b lymphocytes clonally transformed by epstein-barr virus. | somatic rearrangements and deletions of immunoglobulin gene segments have been demonstrated in several types of murine b cells. in addition, rearrangements of the jh, c mu, and light chain immunoglobulin gene segments have been reported in human pre-b-cell leukemias and b-cell lymphomas. we have used recombinant dna probes for the human jh, c mu, and c gamma immunoglobulin gene loci to analyze the genetic events associated with heavy chain gene expression in human b cells clonally transformed by ... | 1985 | 2982163 |
| immune system in rheumatoid arthritis: possible implications in neoplasms. | although the immune system in rheumatoid arthritis is generally in a state of hyperactivity, deficient function can be demonstrated in certain specific parts of the system. this has been most clearly demonstrated in studies of the response of blood lymphocytes from demonstrated in studies of the response of blood lymphocytes from patients with rheumatoid arthritis to in vitro infection by the epstein-barr virus. the virus normally infects b cells promoting proliferation. this results in the esta ... | 1985 | 2982258 |
| malignant lymphoma presenting as kaposi's sarcoma in a homosexual man with the acquired immunodeficiency syndrome. | a homosexual man had kaposi's sarcoma of the skin and lymph nodes. after a brief response to interferon, the patient developed new skin lesions. massive bleeding in the gastrointestinal tract prompted endoscopy, which showed tumor involvement of the stomach, believed to be kaposi's sarcoma. at autopsy, a diffuse lymphoma was found involving the skin and gastrointestinal tract, forming collision tumors in regions that contained kaposi's sarcoma. lymphomatous tissue, but not uninvolved lymph node ... | 1985 | 2982306 |
| nucleic acid probes in diagnosis of viral diseases of man. brief review. | with the recent, rapid advances in recombinant dna technology, it has become possible to consider the use of nucleic acid probes in diagnosis of human viral diseases. several examples are discussed which employ techniques of dot blot hybridization, sandwich hybridization and in situ hybridization. typing of viral strains using restriction endonuclease digestion as an epidemiological tool is considered. finally, the present limitations of molecular hybridization are discussed, and future developm ... | 1985 | 2982349 |
| epstein-barr virus genomes are restricted to secondary neoplastic cells following bone marrow transplantation. | dna from mononuclear blood and tumor cells from 33 patients undergoing bone marrow transplantation for leukemia was examined for the presence of epstein-barr virus (ebv) genomes by blot hybridization. four groups of patients were studied soon after engraftment, during long-term remission, after relapse of the original leukemia, and after development of secondary b cell neoplasms. only the cells of patients with secondary neoplasms demonstrated ebv genomes, where all five adequately studied sampl ... | 1985 | 2982439 |
| opportunistic viral infections. | 1985 | 2982446 | |
| the epstein-barr virus as a model of virus-host interactions. | 1985 | 2982449 | |
| [neutral glycosphingolipids of fabry's disease lymphoblastoid lines established by epstein-barr virus transformation]. | human lymphoid cell lines established by epstein-barr viral transformation of peripheral b-lymphocytes from normal subjects and from fabry patients, were investigated for their ability to biosynthesize neutral glycosphingolipids from [14c]galactose and [14c]glucose as precursors. galactose was taken up in the presence of high concentrations of glucose and selectively utilised by the cells in the synthesis of galactosphingolipids. the pattern of neutral glycosphingolipids labelled from [14c]galac ... | 1985 | 2982612 |
| enhanced expression of epstein-barr virus early antigens by antitubulin agents in a latently infected human lymphoblastoid cell line. | 28 anticancer agents have been surveyed for epstein-barr virus (ebv) activating potency. two vinca alkaloids with antitubulin activity, vinblastine (vlb) and vincristine (vcr), enhanced the expression of ebv early antigens (ea) in a latently infected human lymphoblastoid cell line (raji), when used in combination with n-butyrate. other antitubulin agents, colchicine, colcemid, and podophyllotoxin, had the same effect, although their effects were less than that of the potent tumor promoter, 12-o- ... | 1985 | 2982647 |
| capacity of b-lymphocytic lines of diverse tumor origin to produce and respond to b-cell growth factors: a progression model for b-cell lymphomagenesis. | human cell lines established from cases of acute lymphoblastic leukemia, lymphosarcoma, burkitt's lymphoma and multiple myeloma and representing stages of b-lymphocyte development ranging from pre-b through to plasma cells, were assessed for their ability to produce and respond to b-cell growth factors (bcgf). all b-cell lines studied were found to be constitutive producers of a growth activity which assisted the s-phase entry of normal activated b-cells and provided growth support for lymphobla ... | 1985 | 2982745 |
| effect of cyclosporin-a (csa) on the ability of t lymphocyte subsets to inhibit the proliferation of autologous ebv-transformed b cells. | we have analyzed the effect of cyclosporin-a (csa) on the in vitro suppression of epstein-barr virus (ebv)-induced b-cell proliferations separated on the basis of cell density. csa abolished the growth-inhibitory capacity of high-density t cells but influenced only marginally the activity of low-density lymphocytes; this suggested that different mechanisms mediate suppression in the two subsets. stimulation with irradiated ebv-transformed cells had a different impact on the activity of low- and ... | 1985 | 2982748 |