Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| volatile constituents from the leaves of callicarpa japonica thunb. and their antibacterial activities. | volatile substances of callicarpa japonica thunb. were examined for their antibacterial activities against six foodborne microorganisms using the optical densitometer bioscreen c. extracts of c. japonica were obtained by simultaneous steam distillation and solvent extraction (sde), and those extracted for 1.5 and 2.0 h at ph 6.0 strongly inhibited the growth of bacillus cereus and salmonella typhimurium; the content of the volatile substances of leaves at these ph levels were 543.1 and 706.7 mg/ ... | 2004 | 14969531 |
| a new intrinsic thermal parameter for enzymes reveals true temperature optima. | two established thermal properties of enzymes are the arrhenius activation energy and thermal stability. arising from anomalies found in the variation of enzyme activity with temperature, a comparison has been made of experimental data for the activity and stability properties of five different enzymes with theoretical models. the results provide evidence for a new and fundamental third thermal parameter of enzymes, t(eq), arising from a subsecond timescale-reversible temperature-dependent equil ... | 2004 | 14973131 |
| essential oils: their antibacterial properties and potential applications in foods--a review. | in vitro studies have demonstrated antibacterial activity of essential oils (eos) against listeria monocytogenes, salmonella typhimurium, escherichia coli o157:h7, shigella dysenteria, bacillus cereus and staphylococcus aureus at levels between 0.2 and 10 microl ml(-1). gram-negative organisms are slightly less susceptible than gram-positive bacteria. a number of eo components has been identified as effective antibacterials, e.g. carvacrol, thymol, eugenol, perillaldehyde, cinnamaldehyde and cin ... | 2004 | 15246235 |
| identification of bacillus anthracis by multiprobe microarray hybridization. | we have developed a rapid assay based on microarray analysis of amplified genetic markers for reliable identification of bacillus anthracis and its discrimination from other closely related bacterial species of the bacillus cereus group. by combining polymerase chain reaction (pcr) amplification of six b. anthracis-specific genes (plasmid-associated genes encoding virulence factors (cyaa, paga, lef, and capa, capb, capc) and one chromosomal marker ba-5449) with analysis of amplicons by microarra ... | 2004 | 15246505 |
| mercuric resistance genes in gram-positive oral bacteria. | mercury-resistant bacteria isolated from the oral cavities of children carried one of two types of mera gene that appear to have evolved from a common ancestor. streptococcus oralis, streptococcus mitis and a few other species had mera genes that were very similar to mera of bacillus cereus strain rc607. unlike the b. cereus rc607 mera gene, however, the streptococcal mera genes were not carried on tn5084-like transposons. instead, comparisons with microbial genomic sequences suggest the mera ge ... | 2004 | 15251199 |
| evidence for non-ribosomal peptide synthetase production of cereulide (the emetic toxin) in bacillus cereus. | little is known about the process whereby the emetic toxin (or cereulide) of bacillus cereus is produced. two cereulide-producing strains of b. cereus were cloned and sequenced following polymerase chain reaction (pcr) amplification with primers that were specific for conserved regions of non-ribosomal peptide synthetase (nrps) genes. the cloned regions of the b. cereus strains were highly homologous to conserved regions of other peptide synthetase nucleotide sequences. primers were designed for ... | 2004 | 15251214 |
| the structure of the cell wall peptidoglycan of bacillus cereus rsvf1, a strain closely related to bacillus anthracis. | the peptidoglycan of bacillus cereus rsvf1, a close relative of bacillus anthracis, has several distinguishing features: the overwhelming majority of cross-linked muropeptides are dimers, higher oligomers are only present in minute quantities; and virtually all muropeptides lack the n-acetyl group from glucosamine residues, thus explaining resistance of the cell walls to lysozyme. | 2004 | 15256021 |
| differences among antimicrobial properties of carrion beetle secretions reflect phylogeny and ecology. | carrion beetles (coleoptera: silphidae) consist of two subfamilies in north america. members of the silphinae arrive at carcasses during the mid-stage of decay and their larvae feed on developing maggots, while members of the nicrophorinae bury and tend carcasses upon which their developing larvae feed. the nicrophorinae maintain the condition of the carcass by applying oral and anal secretions that reduce carcass decay apparently through bacterial inhibition, although quantification has not bee ... | 2004 | 15260219 |
| bacillus cereus pneumonia in a patient with acute lymphoblastic leukemia. | reported here is a case of bacillus cereus pneumonia that occurred in a patient with acute lymphoblastic leukemia. the presentation was severe, essentially marked by respiratory distress and pleuritic chest pain. classic empirical treatment initiated for febrile neutropenia did not cover this rare pathogen and appropriate therapy was therefore delayed. b. cereus is most often a culture contaminant, but it can also be responsible for self-limited gastrointestinal intoxication and, more rarely, se ... | 2004 | 15300457 |
| [obtaining of intrapopulational dissociants of some bacilli and the use of dir-pcr for their identification]. | the paper is the first to suggest methods for rapid obtaining and genotypic identification of phenotypic (colonial-morphological) dissociants of bacterial cultures. for revelation of the potential dissociation ability and obtaining of dissociants, the use of bacterial cyst-like refractile cells (crc) is recommended. these cells are characterized by enhanced variability; upon their first passage, an abrupt increase in the dissociation index is observed as a result of the emergence of cells that f ... | 2004 | 15315235 |
| antimicrobial activity and cytotoxicity of the essential oil of curcuma zedoaria. | the chemical compositions of the essential oil of curcuma zedoaria (berg.) rosc. were analyzed by gas chromatography-mass spectrometry (gc-ms) and showed a high content of epicurzerenone and curdione representing 46.6% and 13.7% of the total oil, respectively. the essential oil was evaluated for potential antimicrobial activity against staphylococcus aureus, escherichia coli, pseudomonasa aeruginosa, vibrio parahaemolyticus, salmonella typhimurium and bacillus cereus. v. parahaemolyticus was sen ... | 2004 | 15315265 |
| validation of ph and glucose determination for bacteria detection screening in platelet concentrates stored in the terumo teruflex xt612 platelet container. | 2004 | 15318869 | |
| population structure of the bacillus cereus group as determined by sequence analysis of six housekeeping genes and the plcr gene. | the population structure of the bacillus cereus group (52 strains of b. anthracis, b. cereus, and b. thuringiensis) was investigated by sequencing seven gene fragments (rpob, gyrb, pyca, mdh, mbl, muts, and plcr). most of the strains were classifiable into two large subgroups in six housekeeping gene trees but not in the plcr tree. in addition, several consistent clusters were identified, which were unrelated to species distinction. moreover, interrelationships among these clusters were incongru ... | 2004 | 15322020 |
| evaluation of antibacterial properties of some medicinal plants used in iran. | forty-five species of 29 plant families used in the traditional medicine by iranian people, showed antibacterial activities against one or more of the bacterial species: bacillus cereus, bacillus pumilus, bordetella bronchiseptica, escherichia coli, klebsiella pneumoniae, micrococcus luteus, pseudomonas aeruginosa, pseudomonas fluorescens, serratia marcescens, staphylococcus aureus and staphylococcus epidermidis. no plant showed activity against serratia marcescens; bordetella bronchiseptica bei ... | 2004 | 15325735 |
| a pcr assay based on a sequence-characterized amplified region marker for detection of emetic bacillus cereus. | a pcr assay for the detection of bacillus cereus strains able to produce an emetic toxin (cereulide) was developed in this study based on a sequence-characterized amplified region (scar) derived from a random amplified polymorphic dna (rapd) fragment. one of the rapd fragments generated was selected, cloned, and sequenced. a set of pcr primers was newly designed from the scar obtained (the sequence of the cloned rapd fragment) and used in this assay. to determine the specificity of the assay, 30 ... | 2004 | 15330536 |
| lethality of chlorine, chlorine dioxide, and a commercial fruit and vegetable sanitizer to vegetative cells and spores of bacillus cereus and spores of bacillus thuringiensis. | chlorine, clo2, and a commercial raw fruit and vegetable sanitizer were evaluated for their effectiveness in killing vegetative cells and spores of bacillus cereus and spores of bacillus thuringiensis. the ultimate goal was to use one or both species as a potential surrogate(s) for bacillus anthracis in studies that focus on determining the efficacy of sanitizers in killing the pathogen on food contact surfaces and foods. treatment with alkaline (ph 10.5 to 11.0) clo2 (200 microg/ml) produced by ... | 2004 | 15330537 |
| detection of bacillus cereus on selected retail chicken products. | samples from five chicken meat products, obtained at retail stores, were evaluated for the presence of bacillus cereus. the products tested were as follows: breaded, fully cooked, frozen nuggets (nuggets); breaded, fully cooked, frozen tenders (tenders); fully cooked, frozen, white-meat fajita-style strips (strips); raw, refrigerated, boneless, skinless, marinated breast fillets (fillets); and raw, refrigerated, cut-up, tray-pack bone-in parts (parts), either split breasts or thighs. four packag ... | 2004 | 15330548 |
| antibacterial activities of naturally occurring compounds against antibiotic-resistant bacillus cereus vegetative cells and spores, escherichia coli, and staphylococcus aureus. | after demonstrating the lack of effectiveness of standard antibiotics against the acquired antibiotic resistance of bacillus cereus (nctc 10989), escherichia coli (nctc 1186), and staphylococcus aureus (atcc 12715), we showed that the following natural substances were antibacterial against these resistant pathogens: cinnamon oil, oregano oil, thyme oil, carvacrol, (s)-perillaldehyde, 3,4-dihydroxybenzoic acid (beta-resorcylic acid), and 3,4-dihydroxyphenethylamine (dopamine). exposure of the thr ... | 2004 | 15330549 |
| colony immunoblot assay for the detection of hemolysin bl enterotoxin producing bacillus cereus. | bacillus cereus strains involved in food poisoning cases of the diarrheal type may produce two different enterotoxin complexes. to facilitate the identification of hemolysin bl-enterotoxin complex (hbl) and/or the nonhemolytic enterotoxin (nhe) producing colonies a colony immunoblot procedure was developed, which allows a fast and easy identification of the respective colonies from blood agar plates. the enterotoxins were transferred from the blood agar medium to a nitrocellulose membrane and th ... | 2004 | 15336410 |
| genotyping and toxigenic potential of bacillus subtilis and bacillus pumilus strains occurring in industrial and artisanal cured sausages. | artisanal and industrial sausages were analyzed for their aerobic, heat-resistant microflora to assess whether new emerging pathogens could be present among bacillus strains naturally contaminating cured meat products. sixty-four isolates were characterized by randomly amplified polymorphic dna (rapd)-pcr and fluorescent amplified fragment length polymorphism (faflp). the biotypes, identified by partial 16s rrna gene sequence analysis, belonged to bacillus subtilis, bacillus pumilus, and bacillu ... | 2004 | 15345396 |
| anaerobic growth of bacillus mojavensis and bacillus subtilis requires deoxyribonucleosides or dna. | bacillus mojavensis strains jf-2 (atcc 39307), rob2, and abo21191(t) and bacillus subtilis strains 168 (atcc 23857) and atcc 12332 required four deoxyribonucleosides or dna for growth under strict anaerobic conditions. bacillus licheniformis strains l89-11 and l87-11, bacillus sonorensis strain tg8-8, and bacillus cereus (atcc 14579) did not require dna for anaerobic growth. the requirement for the deoxyribonucleosides or dna did not occur under aerobic growth conditions. the addition of a mixtu ... | 2004 | 15345407 |
| detection and characterization of beta-lactam resistance in bacillus cereus ptcc 1015. | in the present study, detection, isolation, and characterization of beta-lactamases from bacillus cereus ptcc 1015 were investigated. b. cereus was inoculated in nutrient broth containing ampicillin (50 mg x ml(-1)) for 24 h (35 degrees c, 200 rpm). activity measurements were carried out against ampicillin (0.1 mg x ml(-1)) and cephalexin (0.08 mg x ml(-1)) by a spectrophotometric method at different conditions (ph 6-10, temperatures 25-45 degrees c). maximum penicillinase and cephalosporinase a ... | 2004 | 15349504 |
| the nhea component of the non-hemolytic enterotoxin of bacillus cereus is produced by bacillus anthracis but is not required for virulence. | a non-hemolytic enterotoxin (nhe) is one of the two enterotoxins thought to cause diarrhea produced by bacillus cereus. we identified genes in bacillus anthracis homologous to the b. cereus nheab genes encoding proteins of the nhe complex. the nhea component was detected immunologically in culture supernatants from b. anthracis but not from a nhea(-) mutant, suggesting that b. anthracis produces and secretes the nhea subunit of nhe. a nhea deletion mutant was not attenuated in the guinea pig sug ... | 2004 | 15351038 |
| atmospheric oxygen and other conditions affecting the production of cereulide by bacillus cereus in food. | factors influencing the production of cereulide, the emetic toxin of bacillus cereus in food and laboratory media were investigated, using liquid chromatography-ion trap mass spectrometry and sperm motility inhibition bioassay for detection and quantitation. oxygen was essential for production of the emetic toxin by b. cereus. when beans, rice or tryptic soy broth were inoculated with cereulide producing strains b203, b116 (recent food isolates) or the strain f-4810/72, high amounts (2 to 7 micr ... | 2004 | 15358508 |
| glycopeptide derived from hen egg ovomucin has the ability to bind enterohemorrhagic escherichia coli o157:h7. | ovomucin glycopeptide (ogp) was prepared by size exclusion chromatography after pronase digestion of hen egg ovomucin, and the binding of ogp to foodborne pathogens (bacillus cereus,clostridium perfringens, escherichia coli o157:h7, listeria monocytogenes, salmonella enteritidis, salmonella typhimurium, and staphylococcus aureus) was investigaed. binding assays with biotinylated bacteria as probes in microtiter plates showed that ogp bound to only e. coli o157:h7 among these foodborne pathogens. ... | 2004 | 15373418 |
| mesophilic aerobic degradation of a metal lubricant by a biological consortium. | the metal-forming industries require the use of greases to lubricate metal surfaces during manufacturing operations, and the residues of these lubricants must be removed prior to finishing processes to protect and improve the appearance of the final product. an aqueous, biological metal-cleaning process operating under mild conditions (ph 9, 42 degrees c) eliminates the use of environmentally unfriendly cleaning materials such as chlorinated solvents by employing microorganisms to degrade grease ... | 2004 | 15378296 |
| a targeted proteomics approach to the rapid identification of bacterial cell mixtures by matrix-assisted laser desorption/ionization mass spectrometry. | a proteomic approach to the rapid identification of bacteria is presented, which relies on the solubilization of a limited number of proteins from intact cells combined with on-probe tryptic digestion. within 20 min, complete cleavage products of a limited set of bacterial proteins with molecular masses of about 4-125 kda were obtained by on-probe digestion with immobilized trypsin. bacterial peptides suitable for unimolecular decomposition analysis were generated within 5 min, and the sequence ... | 2004 | 15378756 |
| indigenous bacteria with antagonistic and plant-growth-promoting activities improve slow-filtration efficiency in soilless cultivation. | in tomato soilless culture, slow filtration allows one to control the development of diseases caused by pathogenic microorganisms. during the disinfecting process, microbial elimination is ensured by mechanical and biological factors. in this study, system efficacy was enhanced further to a biological activation of filter by inoculating the pozzolana grains contained in the filtering unit with 5 selected bacteria. three strains identified as pseudomonas putida and 2 as bacillus cereus came from ... | 2004 | 15381975 |
| photochemical treatment of platelet concentrates with amotosalen and long-wavelength ultraviolet light inactivates a broad spectrum of pathogenic bacteria. | bacterial contamination of platelet (plt) concentrates can result in transfusion-transmitted sepsis. a photochemical treatment (pct) process with amotosalen hcl and long-wavelength ultraviolet light (uva), which cross-links nucleic acids, was developed to inactivate bacteria and other pathogens in plt concentrates. | 2004 | 15383024 |
| a spontaneous translational fusion of bacillus cereus plcr and papr activates transcription of plcr-dependent genes in bacillus anthracis via binding with a specific palindromic sequence. | transformation of bacillus anthracis with plasmid pute29-plcr-papr carrying the native bacillus cereus plcr-papr gene cluster did not activate expression of b. anthracis hemolysin genes, even though these are expected to be responsive to activation by the global regulator plcr. to further characterize the action of plcr, we examined approximately 3,000 b. anthracis transformants containing pute29-plcr-papr and found a single hemolytic colony. the hemolytic strain contained a plasmid having a spo ... | 2004 | 15385482 |
| characterization and virulence of hemolysin iii from vibrio vulnificus. | vibrio vulnificus, a highly virulent marine bacterium, is the causative agent of both serious wound infections and fatal septicemia in many areas of the word. a gene (hlyiii) encoding a hemolysin was cloned and sequenced from v. vulnificus. nucleotide sequence analysis predicted an open reading frame of 642 bp encoding a 214 amino acid polypeptide that showed 48% sequence identity to the hemolysin iii of bacillus cereus. when hlyiii of v. vulnificus was expressed in escherichia coli, crude extra ... | 2004 | 15386100 |
| validation of bact/alert plastic culture bottles for use in testing of whole-blood-derived leukoreduced platelet-rich-plasma-derived platelets. | bacterial detection of platelet (plt)-rich-plasma (prp)-derived plts presents unique challenges for countries that do not allow pooling before storage. this study validated the bact/alert for use in testing pooled prp-derived plts with nine contaminating organisms. | 2004 | 15265121 |
| biocontrol of psychrotrophic enterotoxigenic bacillus cereus in a nonfat hard cheese by an enterococcal strain-producing enterocin as-48. | bacillus cereus is a food poisoning bacterium of great concern, especially in milk products. in this study, we describe the efficient control of the psychrotrophic and toxigenic strain b. cereus lwl1 in milk and in a nonfat hard cow's cheese by the enterocin as-48 producer strain enterococcus faecalis a-48-32 (bac+). no viable b. cereus cells were detected after 72 h incubation in milk coinoculated with the as-48-producing strain and b. cereus. diarrheic toxin production was also markedly inhibi ... | 2004 | 15270513 |
| application of oligonucleotide array technology for the rapid detection of pathogenic bacteria of foodborne infections. | a rapid and accurate method for detection for common pathogenic bacteria in foodborne infections was established by using oligonucleotide array technology. nylon membrane was used as the array support. a mutation region of the 23s rrna gene was selected as the discrimination target from 14 species (genera) of bacteria causing foodborne infections and two unrelated bacterial species. a pair of universal primers was designed for pcr amplification of the 23s rrna gene. twenty-one species (genera)-s ... | 2004 | 15279944 |
| cloning and partial characterization of zwittermicin a resistance gene cluster from bacillus thuringiensis subsp. kurstaki strain hd1. | the study seeks to shed light on the aminopolyol, broad-spectrum antibiotic zwittermicin a gene cluster of bacillus thuringiensis subsp. kurstaki hd1 and to identify any new uncharacterized genes with an eventual goal to establish a better understanding of the resistance gene cluster. | 2004 | 15281929 |
| three cases of post-traumatic endophthalmitis caused by unusual bacteria. | three cases of post-traumatic endophthalmitis caused by unusual bacteria are presented. the pathogens identified were: (i) bacillus cereus and citrobacter freundii; (ii) pseudomonas fluorescens; and (iii) chryseobacterium meningosepticum and stenotrophomonas maltophilia. two of these pathogens have not previously been reported to cause endophthalmitis. the available literature regarding the individual cases is summarized and a brief discussion of post-traumatic endophthalmitis is presented, with ... | 2004 | 15281988 |
| use of a fluorescent viability stain to assess lethal and sublethal injury in food-borne bacteria exposed to high-intensity pulsed electric fields. | to apply scanning electron microscopy, image analysis and a fluorescent viability stain to assess lethal and sublethal in food-borne bacteria exposed to high-intensity pulsed electric fields (pef). | 2004 | 15287869 |
| genetic and functional analysis of the cytk family of genes in bacillus cereus. | cytk is a pore-forming toxin of bacillus cereus that has been linked to a case of necrotic enteritis. pcr products of the expected size were generated with cytk primers in 13 of 29 strains. six strains were pcr-positive for the related gene hly-ii, which encodes haemolysin ii, a protein that is 37 % identical to the original cytk. five of the strains were positive for both genes. the dna sequences of putative cytk genes from three positive strains were determined, and the deduced amino acid sequ ... | 2004 | 15289565 |
| comparison of cytotoxin cytk promoters from bacillus cereus strain atcc 14579 and from a b. cereus food-poisoning strain. | the cytotoxin cytk produced by bacillus cereus is believed to be involved in food-borne diseases. the transcriptional activity of the cytk promoter region in a food-poisoning strain was studied using a reporter gene and compared with that in the reference b. cereus strain atcc 14579. in the food-poisoning strain, cytk is more strongly transcribed, possibly explaining the pathogenicity. the global regulator plcr in b. cereus controls several putative virulence factors. it was found that plcr regu ... | 2004 | 15289566 |
| laundry detergent compatibility of the alkaline protease from bacillus cereus. | the endogenous protease activity in various commercially available laundry detergents of international companies was studied. the maximum protease activity was found at 50 degrees c in ph range 10.5-11.0 in all the tested laundry detergents. the endogenous protease activity in the tested detergents retained up to 70% on incubation at 40 degrees c for 1 h, whereas less than 30% activity was only found on incubation at 50 degrees c for 1 h. the alkaline protease from an alkalophilic strain of baci ... | 2004 | 15293947 |
| mics of selected antibiotics for bacillus anthracis, bacillus cereus, bacillus thuringiensis, and bacillus mycoides from a range of clinical and environmental sources as determined by the etest. | this paper presents etest determinations of mics of selected antimicrobial agents for 76 isolates of bacillus anthracis chosen for their diverse histories and 67, 12, and 4 cultures, respectively, of its close relatives b. cereus, b. thuringiensis, and b. mycoides derived from a range of clinical and environmental sources. nccls breakpoints are now available for b. anthracis and ciprofloxacin, penicillin, and tetracycline; based on these breakpoints, the b. anthracis isolates were all fully susc ... | 2004 | 15297508 |
| use of 16s rrna, 23s rrna, and gyrb gene sequence analysis to determine phylogenetic relationships of bacillus cereus group microorganisms. | in order to determine if variations in rrna sequence could be used for discrimination of the members of the bacillus cereus group, we analyzed 183 16s rrna and 74 23s rrna sequences for all species in the b. cereus group. we also analyzed 30 gyrb sequences for b. cereus group strains with published 16s rrna sequences. our findings indicated that the three most common species of the b. cereus group, b. cereus, bacillus thuringiensis, and bacillus mycoides, were each heterogeneous in all three gen ... | 2004 | 15297521 |
| assessment of a new selective chromogenic bacillus cereus group plating medium and use of enterobacterial autoinducer of growth for cultural identification of bacillus species. | a new chromogenic bacillus cereus group plating medium permits differentiation of pathogenic bacillus species by colony morphology and color. probiotic b. cereus mutants were distinguished from wild-type strains by their susceptibilities to penicillin g or cefazolin. the enterobacterial autoinducer increased the sensitivity and the speed of enrichment of b. cereus and b. anthracis spores in serum-supplemented minimal salts medium (based on the standard american petroleum institute medium) and bu ... | 2004 | 15297532 |
| [the protective effect of supplementation of probiotics combined with riboflavin on the intestinal barrier of the rats after scald injury]. | to investigate the effect and its possible mechanism of the supplementation of probiotics combined with riboflavin on the intestinal barriers of the rats after scald injury. | 2004 | 15447817 |
| engineering of the ph optimum of bacillus cereus beta-amylase: conversion of the ph optimum from a bacterial type to a higher-plant type. | the optimum ph of bacillus cereus beta-amylase (bcb, ph 6.7) differs from that of soybean beta-amylase (sba, ph 5.4) due to the substitution of a few amino acid residues near the catalytic base residue (glu 380 in sba and glu 367 in bcb). to explore the mechanism for controlling the optimum ph of beta-amylase, five mutants of bcb (y164e, y164f, y164h, y164q, and y164q/t47m/y164e/t328n) were constructed and characterized with respect to enzymatic properties and x-ray structural crystal analysis. ... | 2004 | 15449941 |
| a subnanogram assay for phospholipase activity based on a long-chain radioiodinatable phosphatidylcholine. | here, we introduce a radioiodinatable long-chain phosphatidylcholine (bhc12pc) which serves as the base for a very sensitive phospholipase assay. this compound has a 4-hydroxyphenyl group attached at the end of the fatty acyl chain located in position sn-2. this feature enables this phospholipid to be radioiodinated. bhc12pc was tested as a substrate of naja naja naja pla(2) and bacillus cereus plc in a mixed micellar system with triton x-100. the detection limit for the assays was 0.25ng of pla ... | 2004 | 15450804 |
| water-assisted reaction mechanism of monozinc beta-lactamases. | hybrid car-parrinello qm/mm calculations are used to investigate the reaction mechanism of hydrolysis of a common beta-lactam substrate (cefotaxime) by the monozinc beta-lactamase from bacillus cereus (bcii). the calculations suggest a fundamental role for an active site water in the catalytic mechanism. this water molecule binds the zinc ion in the first step of the reaction, expanding the zinc coordination number and providing a proton donor adequately oriented for the second step. the free en ... | 2004 | 15453800 |
| phenolic compounds from baseonema acuminatum leaves: isolation and antimicrobial activity. | three new phenolic compounds, 1-galloyl-beta-d-glucopyranosyl-(1-->4)-beta-d-galactopyranoside (1), 2-methoxy-5-(1 '2 3'-trihydroxypropyl)-phenyl- 1-0-(6"-galloyl)-beta-d-glucopyranoside (2),and 2-methoxy-5-hydroxymethyl-phenyl-1-o-(6"-galloyl)-beta-d-glucopyranoside (3), together with the known compounds benzyl 6'-o-galloyl-beta-d-glucopyranoside (4), 1,6-di-o-galloyl-beta-d-glucopyranose (5), myrciaphenone b (6), kaempferol 3-0-(6"-galloyl)-beta-d-glucopyranoside (7), quercetin 3-0-(6"-galloyl ... | 2004 | 15503354 |
| boiling and bacillus spores. | 2004 | 15515252 | |
| effect of culture conditions on the achievable taxonomic resolution of raman spectroscopy disclosed by three bacillus species. | confocal micro-raman spectroscopy requires a minimum of sample handling and no reagents and allows fast identification of microorganisms. since it reflects the overall molecular composition of the cells, it provides much more information than classical, microbial analyses. however, since the molecular makeup of a cell depends on culture conditions, it can be argued that this will affect the reproducibility and discrimination ability of raman spectroscopy. we used bacillus cereus, bacillus pumilu ... | 2004 | 15516118 |
| the bcr1 dna repeat element is specific to the bacillus cereus group and exhibits mobile element characteristics. | bacillus cereus strains atcc 10987 and atcc 14579 harbor an approximately 155-bp repeated element, bcr1, which is conserved in b. cereus, b. anthracis, b. thuringiensis, and b. mycoides but not in b. subtilis and b. licheniformis. in this study, we show by southern blot hybridizations that bcr1 is present in all 54 b. cereus group strains tested but absent in 11 bacillus strains outside the group, suggesting that bcr1 may be specific and ubiquitous to the b. cereus group. by comparative analysis ... | 2004 | 15516586 |
| chromogenic assay for the activity of sphingomyelinase from bacillus cereus and its application to the enzymatic hydrolysis of lysophospholipids. | we developed a convenient chromogenic assay method for the activity of sphingomyelinase (smase) from bacillus cereus. smase reaction was quenched by zn(2+), and the released phosphocholine was converted into a choline by the action of alkaline phosphatase. after that, the choline was converted into a chromogenic dye by the actions of choline oxidase and peroxidase in the presence of edta to trap the added zn(2+) which could interfere with the choline oxidase/peroxidase reactions. triton x-100 al ... | 2004 | 15516713 |
| chemical composition and in vitro antimicrobial activities of the essential oils from endemic psiadia species growing in mauritius. | the composition of the leaf oils, obtained by hydrodistillation, of five endemic psiadia species of the asteraceae family were studied by gc/ms on both polar and non-polar columns. the analysis showed that the volatile components of the oils were made up essentially of monoterpenes, sesquiterpenes, aliphatics and other shikimic acid derivatives. with respect to the non-volatile components, great variations were observed: p. lithospermifolia contained (e)-isoasarone (51.5%); p. penninervia: eugen ... | 2004 | 15516729 |
| oligonucleotide microarray for identification of bacillus anthracis based on intergenic transcribed spacers in ribosomal dna. | we developed a dna microarray for identification of bacillus anthracis and other phylogenetic groupings within the "bacillus cereus group". nucleotide sequences of 16s-23s ribosomal dna internal transcribed spacers containing genes for trna(ile) from 52 b. anthracis strains were found to be identical to sequences from seven strains published previously and different from all other bacteria. when 42 oligonucleotide probes targeting polymorphic sites were immobilized on glass slides and hybridized ... | 2004 | 15522510 |
| a practical synthesis of gramicidin s and sugar amino acid containing analogues. | a practical gram-scale and high-yielding synthesis of the antimicrobial peptide gramicidin s is presented. an fmoc-based solid-phase peptide synthesis protocol is employed for the generation of the linear decapeptide precursor, which is cyclized in solution to afford the target compound. the versatility of our method is demonstrated by the construction of eight gramicidin s analogues (15a-h) having nonproteinogenic sugar amino acid residues (4-7) incorporated in the turn regions. | 2004 | 15527261 |
| growth inhibition of foodborne pathogens and food spoilage organisms by select raw honeys. | twenty-seven honey samples from different floral sources and geographical locations were evaluated for their ability to inhibit the growth of seven food spoilage organisms (alcaligenes faecalis, aspergillus niger, bacillus stearothermophilus, geotrichum candidum, lactobacillus acidophilus, penicillium expansum, pseudomonas fluorescens) and five foodborne pathogens (bacillus cereus, escherichia coli o157:h7, listeria monocytogenes, salmonella enterica ser. typhimurium, and staphylococcus aureus) ... | 2004 | 15527912 |
| antimicrobial effect of water extract of sumac (rhus coriaria l.) on the growth of some food borne bacteria including pathogens. | the antimicrobial effect of water extracts of sumac (rhus coriaria l.) at concentrations of 0.1%, 0.5%, 1.0%, 2.5% and 5.0% (w/v), non-neutralized and after neutralization to ph 7.2+/-0.1, was studied on the growth of 12 bacterial strains (six gram positive strains and six gram negative strains), mostly food borne including pathogens. it was found to be effective against all the test organisms with gram positive strains being more sensitive than gram negative strains. significant differences (p< ... | 2004 | 15527919 |
| new vector for efficient allelic replacement in naturally nontransformable, low-gc-content, gram-positive bacteria. | a shuttle vector designated pmad was constructed for quickly generating gene inactivation mutants in naturally nontransformable gram-positive bacteria. this vector allows, on x-gal (5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside) plates, a quick colorimetric blue-white discrimination of bacteria which have lost the plasmid, greatly facilitating clone identification during mutagenesis. the plasmid was used in staphylococcus aureus, listeria monocytogenes, and bacillus cereus to efficiently c ... | 2004 | 15528558 |
| a new bacillus cereus dna-binding protein, hlyiir, negatively regulates expression of b. cereus haemolysin ii. | haemolysin ii, hlyii, is one of several cytotoxic proteins produced by bacillus cereus, an opportunistic human pathogen that causes food poisoning. the hlyii gene confers haemolytic activity to escherichia coli cells. here a new b. cereus gene, hlyiir, which is located immediately downstream of hlyii and regulates hlyii expression, is reported. the deduced amino acid sequence of hlyiir is similar to prokaryotic dna-binding transcriptional regulators of the tetr/acra family. measurements of haemo ... | 2004 | 15528656 |
| bacillus cereus, the causative agent of an emetic type of food-borne illness. | bacillus cereus is the causative agent of two distinct forms of gastroenteritic disease connected to food-poisoning. it produces one emesis-causing toxin and three enterotoxins that elicit diarrhea. due to changing lifestyles and eating habits, b. cereus is responsible for an increasing number of food-borne diseases in the industrial world. in the past, most studies concentrated on the diarrhoeal type of food-borne disease, while less attention has been given to the emetic type of the disease. t ... | 2004 | 15538709 |
| [interaction of neomycin with other antibiotics on selected bacterial strains]. | antimicrobial combinations are used most frequently to provide broad-spectrum empirical coverage in the treatment of bacterial infections. however, combination of two antibiotics may not influence their activity, may lead to synergy or antagonism in the activity. neomycin may be combined with one of the following antibiotics: ampicillin, procaine penicillin, gramicidin, bacitracin, polymyxin b, lincomycin, oxytetracycline, and erythromycin in some human and veterinary multiantibiotic drugs distr ... | 2004 | 15544091 |
| association between tea ingestion and invasive bacillus cereus infection among children with cancer. | bacillus cereus is an emerging pathogen that causes invasive disease in immunocompromised hosts. a case-control study, prompted by a clinical case, demonstrated an association between dietary tea ingestion and b. cereus bacteremia. policies designed to interrupt transmission of this pathogen to susceptible patients should be considered. | 2004 | 15546093 |
| description of heterotrophic bacteria occurring in paper mills and paper products. | to isolate aerobic mesophilic bacilli and thermophilic bacteria from different paper mill samples and to evaluate their potential harmfulness. | 2004 | 15546414 |
| population structure and evolution of the bacillus cereus group. | representative strains of the bacillus cereus group of bacteria, including bacillus anthracis (11 isolates), b. cereus (38 isolates), bacillus mycoides (1 isolate), bacillus thuringiensis (53 isolates from 17 serovars), and bacillus weihenstephanensis (2 isolates) were assigned to 59 sequence types (sts) derived from the nucleotide sequences of seven alleles, glpf, gmk, ilvd, pta, pur, pyca, and tpi. comparisons of the maximum likelihood (ml) tree of the concatenated sequences with individual ge ... | 2004 | 15547268 |
| spectroscopic quantification of bacteria using artificial neural networks. | fourier transform-infrared spectroscopy, in conjunction with artificial neural networks, has been used for identification and classification of selected foodborne pathogens. five bacterial species (enterococcus faecium, salmonella enteritidis, bacillus cereus, yersinia enterocolitica, shigella boydii) and five escherichia coli strains (o103, o55, o121, o30, o26) suspended in phosphate-buffered saline were enumerated to provide seven different concentrations ranging from 10(9) to 10(3) cfu/ ml. t ... | 2004 | 15553640 |
| detection and identification of bacteria in a juice matrix with fourier transform-near infrared spectroscopy and multivariiate analysis. | the use of fourier transform-near infrared (ft-nir) spectroscopy combined with multivariate pattern recognition techniques was evaluated to address the need for a fast and senisitive method for the detection of bacterial contamination in liquids. the complex cellular composition of bacteria produces ft-nir vibrational transitions (overtone and combination bands), forming the basis for identification and subtyping. a database including strains of escherichia coli, pseudomonas aeruginosa, bacillus ... | 2004 | 15553641 |
| determination of thermal inactivation kinetics of microorganisms with a continuous microflow apparatus. | use of a continuous microflow submerged microcoil (csmc) apparatus was compared with the capillary tube (ct) method for measuring the thermal inactivation kinetics of pseudomonas fluorescens at 61 degrees c for 3 to 29 s. inocula were continuously pumped through a microbore (< or = 0.0762 cm inside diameter) thin-walled stainless steel capillary tube submerged in a heated oil bath. the heating time was set by changing the flow rate, tube dimensions, or both. with the use of microthermo-couples, ... | 2004 | 15553642 |
| [microbiological method for the detection of antibiotic residues in meat using mixed-mode, reverse-phase and cation-exchange cartridge]. | a microbiological method for screening of residual benzylpenicillin (pcg), oxytetracycline (otc) and spiramycin (spm) in meat using a single mixed mode, reversed-phase and cation-exchange cartridge was developed. a meat sample was extracted with 0.1 mol/ml ph 4.5 phosphate buffer and the extract was applied to a mcx cartridge. the cartridge was washed, and adsorbed antibiotic residues were eluted with acetonitrile for acidic fractions and acetonitrile containing 5% ammonia solution-0.1 mol/ml ph ... | 2004 | 15568469 |
| failure of intravitreal dexamethasone to diminish inflammation or retinal toxicity in an experimental model of bacillus cereus endophthalmitis. | to determine whether the usual clinical dose of intravitreal dexamethasone can attenuate intraocular inflammation and retinal necrosis in a rabbit model of fulminant bacillus cereus endophthalmitis induced by crude exotoxins. | 2004 | 15590470 |
| synthesis and study of antibacterial and antifungal activities of novel 2-[[(benzoxazole/benzimidazole-2-yl)sulfanyl] acetylamino]thiazoles. | several 2-[[(benzoxazole/benzimidazole-2-yl)sulfanyl]acetylamino]thiazoles derivatives were synthesized by reacting 4-substituted-2-(chloroacetylamino)thiazoles with benzoxazole/benzimidazole-2-thioles in acetone and in the presence of k2co3. the chemical structures of the compounds were elucidated by ir, 1h-nmr, and fab(+)-ms spectral data. their antimicrobial activities against micrococcus luteus (nrll b-4375), bacillus cereus (nrrl b-3711), proteus vulgaris (nrrl b-123), salmonella typhimuriu ... | 2004 | 15595406 |
| inorganic polyphosphate in bacillus cereus: motility, biofilm formation, and sporulation. | chains of inorganic polyphosphate (poly-p) with hundreds of p(i) residues linked by phosphoanhydride bonds, as in atp, are found in every bacterial, fungal, plant, and animal cell, in which they perform various functions. in the spore-forming bacillus cereus, we have identified three principal enzymes and genes involved in the metabolism of poly-p, namely, (i) poly-p kinase (ppk), which synthesizes poly-p reversibly from atp, (ii) exopolyphosphatase (ppx), which hydrolyzes poly-p to p(i), and (i ... | 2004 | 15572452 |
| glycoconjugates for the recognition of bacillus spores. | carbohydrates act as ligands in many biological processes, including the folding and secretion of proteins, cell-cell recognition, adhesion, and sporulation in the bacillus genus. fluorescent-labeled disaccharide glycoconjugates have been applied to evaluate binding to bacterial spores assuming that the spore surface is covered with carbohydrates. this study has shown that specific recognition of bacterial spores is based on interactions between disaccharide glycoconjugates acting as ligands and ... | 2004 | 15582612 |
| characterization of the bacillus cereus nhe enterotoxin. | the non-haemolytic enterotoxin (nhe) is one of two three-component enterotoxins responsible for the diarrhoeal food-poisoning syndrome caused by bacillus cereus. nhe is composed of nhea, nheb and nhec. the three genes encoding the nhe components constitute an operon, and the transcriptional start site is located 61 bp upstream of the nhea translational start site. the nhe genes were cloned separately, and expressed in either bacillus subtilis or escherichia coli. separate expression showed that ... | 2004 | 15583149 |
| real-time pcr assay for a unique chromosomal sequence of bacillus anthracis. | real-time pcr has become an important method for the rapid identification of bacillus anthracis since the 2001 anthrax mailings. most real-time pcr assays for b. anthracis have been developed to detect virulence genes located on the pxo1 and pxo2 plasmids. in contrast, only two published chromosomal targets exist, the rpob gene and the gyra gene. in the present study, subtraction-hybridization with a plasmid-cured b. anthracis tester strain and a bacillus cereus driver was used to find a unique ... | 2004 | 15583318 |
| thermophilic, reversible gamma-resorcylate decarboxylase from rhizobium sp. strain mtp-10005: purification, molecular characterization, and expression. | we found the occurrence of thermophilic reversible gamma-resorcylate decarboxylase (gamma-rdc) in the cell extract of a bacterium isolated from natural water, rhizobium sp. strain mtp-10005, and purified the enzyme to homogeneity. the molecular mass of the enzyme was determined to be about 151 kda by gel filtration, and that of the subunit was 37.5 kda by sodium dodecyl sulfate-polyacrylamide gel electrophoresis; in other words, the enzyme was a homotetramer. the enzyme was induced specifically ... | 2004 | 15466039 |
| quorum quenching: enzymatic disruption of n-acylhomoserine lactone-mediated bacterial communication in burkholderia thailandensis. | many species of gram-negative bacteria communicate by synthesizing, secreting, and responding to n-acylhomoserine lactones (ahls), a mechanism termed quorum sensing. several investigations have characterized numerous ahl-degrading enzymes (aiia lactonases) encoded by environmental isolates of bacillus spp. the burkholderia thailandensis quorum system is comprised of at least three ahl synthases (ahss) and five transcriptional regulators belonging to the luxir class of proteins. expression of the ... | 2004 | 15466564 |
| a rapid bioassay for single bacterial cell quantitation using bioconjugated nanoparticles. | the rapid and sensitive determination of pathogenic bacteria is extremely important in biotechnology, medical diagnosis, and the current fight against bioterrorism. current methods either lack ultrasensitivity or take a long time for analysis. here, we report a bioconjugated nanoparticle-based bioassay for in situ pathogen quantification down to single bacterium within 20 min. the bioconjugated nanoparticle provides an extremely high fluorescent signal for bioanalysis and can be easily incorpora ... | 2004 | 15477593 |
| cereulide, the emetic toxin of bacillus cereus, is putatively a product of nonribosomal peptide synthesis. | to determine if cereulide, the emetic toxin produced by bacillus cereus, is produced by a nonribosomal peptide synthetase (nrps). | 2004 | 15479414 |
| [selective nutrient medium for the isolation of bacillus cereus]. | a selective dried medium for the isolation of b. cereus from clinical material and foodstuffs has been developed. the medium has high selective properties which ensure the isolation of b. cereus from microbial association in pure culture, thus making it possible to accelerate further identification of the microorganisms. | 2004 | 15481930 |
| enhanced specificity of bacterial spore identification by oxidation and mass spectrometry. | addition of an oxidizing agent (e.g., hydrogen peroxide) to intact spores selectively and completely oxidizes met-containing biomarker proteins by formation of met sulfoxides. this reaction increases the masses of the biomarker proteins observed in matrix-assisted laser desorption/ionization mass spectrometry (maldi-ms) of bacillus spores by deltam = (16 x n) da, where n is the number of met residues in the sequence of each individual protein. the procedure is very rapid, and can be performed in ... | 2004 | 15490431 |
| molecular characterisation of bacterial contamination in semi-final gelatine extracts, using denaturing gradient gel electrophoresis. | contamination of gelatine may affect the safety and/or quality of its applications. characterisation of bacterial isolates from semi-final gelatine batches revealed thermotolerant, aerobic, endosporeforming contaminants. in this paper, bacterial contamination in gelatine batches is analysed without previous isolation, by means of denaturing gradient gel electrophoresis (dgge) of pcr-amplified 16s rdna sequences. v9 and v6-v8 regions of the 16s rdna gene were found more suitable for this purpose ... | 2004 | 15490563 |
| [microbiological quality of minimally processed vegetable salads]. | the microbiological condition of minimally processed vegetable salads (7-days durability) purchased in the retail network of szczecin has been assessed. the study included 14 kinds of salads manufactured by two producers. the total count of mesophilic aerobic bacteria and their spores, aid-forming bacteria (lactobacilli), titre of coliforms, occurrence of pathogenic bacteria and counts of moulds and yeast were determined. no pathogenic bacteria (salmonella, staphylococcus aureus, bacillus cereus ... | 2004 | 15493346 |
| food safety: emerging trends in foodborne illness surveillance and prevention. | between 250 and 350 million americans are estimated to suffer acute gastroenteritis annually, with 25% to 30% thought to be caused by foodborne illnesses. most vulnerable to foodborne diseases are elderly people, pregnant women, immune-compromised people, and children. while bacterial causes such as salmonella are widely recognized and monitored as foodborne infections, other important bacterial causes such as clostridium perfringens , bacillus cereus , and staphylococcus aureus are less well kn ... | 2004 | 15499359 |
| screening of antimicrobial activity of diarylamines in the 2,3,5-trimethylbenzo[b]thiophene series: a structure-activity evaluation study. | gram positive (bacillus cereus, b. subtilis), gram negative (pseudomonas aeruginosa, escherichia coli) bacteria, and candida albicans as a representative of fungi were used for screening the in vitro antimicrobial activity of diarylamines in the 2,3,5-trimethylbenzo[b]thiophene series bearing different substituents, synthesized by us using the palladium-catalyzed c-n coupling methodology. the minimal inhibitory concentration (mic) and structure-activity relationships (sars) were evaluated. | 2004 | 15501050 |
| high-level expression of recombinant phospholipase c from bacillus cereus in pichia pastoris and its characterization. | the phospholipase c (plc) gene from bacillus cereus was cloned into the ppiczc vector and integrated into the genome of pichia pastoris. the phospholipase c (plc) when expressed in p. pastoris was fused to the alpha-factor secretion signal peptide of saccharomyces cerevisiae and secreted into a culture medium. recombinant p. pastoris x-33 had a clear plc band at 28.5 kda and produced an extracellular plc with an activity of 678 u mg(-1) protein which was more than a recombinant p. pastoris gs115 ... | 2004 | 15604782 |
| enzymatic properties of the membrane-bound nadh oxidase system in the aerobic respiratory chain of bacillus cereus. | membranes prepared from bacillus cereus kctc 3674, grown aerobically on a complex medium, oxidized nadh exclusively, whereas deamino-nadh was little oxidized. the respiratory chain-linked nadh oxidase exhibited an apparent k(m) value of approximately 65 microm for nadh. the maximum activity of the nadh oxidase was obtained at about ph 8.5 in the presence of 0.1 m kcl (or nacl). respiratory chain inhibitor 2-heptyl-4-hydroxyquinoline-n-oxide (hqno) inhibited the activity of the nadh oxidase by ab ... | 2004 | 15607037 |
| essential oils of satureja, origanum, and thymus species: chemical composition and antibacterial activities against foodborne pathogens. | the chemical composition of the essential oils obtained from the species restricted to greece and the eastern mediterranean region, satureja spinosa l. and thymus longicaulis l.; species endemic to central and south greece, satureja parnassica ssp. parnassica heldr. and sart ex boiss.; species endemic to the island of crete, origanum dictamnus l.; and species widely distributed in the mediterranean region, satureja thymbra l. and origanum vulgare l. subsp. hirtum, were determined by gas chromato ... | 2004 | 15612827 |
| characterization and antimicrobial activity of bacteriocin 217 produced by natural isolate lactobacillus paracasei subsp. paracasei bgbuk2-16. | the strain lactobacillus paracasei subsp. paracasei bgbuk2-16. which was isolated from traditionally homemade white-pickled cheese, produces bacteriocin 217 (bac217; approximately 7 kda). the onset of bac217 biosynthesis was observed in the logarithmic phase of growth, and the production plateau was reached after 9 or 12 h of incubation at 37 and 30 degrees c, respectively, when culture entered the early stationary phase. biochemical characterization showed that bac217 retained antimicrobial act ... | 2004 | 15633678 |
| incidence and characterization of bacillus cereus isolated from traditional fermented meals in nigeria. | the aim of this study was to examine the presence of bacillus cereus in fermented meals used in food seasoning in nigeria. the microbial profiles of iru and ogiri, two nigerian fermented vegetable proteins, were examined for presence of b. cereus. in the 50 samples tested, b. cereus was detected in all the samples, with the level of detection ranging from log 6.3 to log 8.3 g(-1) sample. phenotypic characteristics of the b. cereus isolates showed that all of them could not ferment many sugars, m ... | 2004 | 15633691 |
| synthesis and antibacterial activity of 4-benzoyl-1-methyl-5-phenyl-1h-pyrazole-3-carboxylic acid and derivatives. | some new 1h-pyrazole-3-carboxylic acid and pyridazinone derivatives were synthesized and evaluated for their antibacterial activities against bacillus cereus atcc 7064, staphylococcus aureus atcc 6538, escherichia coli atcc 4230 and pseudomonas putida using tube dilution method. the minimal inhibitory concentrations (mics) experiments revealed that all chemical compounds showed inhibitor effects on the growth of the test microorganisms. moreover, the results of this research showed that the comp ... | 2004 | 15652364 |
| pollution status of swimming pools in south-south zone of south-eastern nigeria using microbiological and physicochemical indices. | microbiological and physicochemical characteristics of swimming pools in south eastern states of nigeria (akwa ibom and cross river) were investigated. the bacterial isolates included enterococcus faecalis, clostridium perfringens, bacillus cereus and escherichia coli. others were pseudomonas aeruginosa, staphylococcus aureus, proteus vulgaris and staphylococcus epidermidis, while fungal isolates were penicillium sp, rhizopus sp, aspergillus versicolor fusarium sp, trichophyton mentagrophytes, m ... | 2004 | 15691161 |
| antibacterial activity of an eudesmane sesquiterpene isolated from common varthemia, varthemia iphionoides. | the ethyl acetate extract of the whole aerial parts of varthemia iphionoides (compositae) showed a pronounced antibacterial activity. the bioassay-guided fractionation of the ethyl acetate extract and further purification of the most antibacterially active fraction led to the isolation and identification of an antibacterial sesquiterpene, selina-4,11(13)-dien-3-on-12-oic acid. this compound exhibited potent antimicrobial activity against six bacterial species (staphylococcus aureus, bacillus sub ... | 2004 | 15707759 |
| antagonistic effects of several bacteria on fusarium oxysporum, the causal agent of root and crown rot of onion under field conditions. | onion (allium cepa) is one of the most important vegetable crop which is commonly used as a food supplement. this plant is found to be vulnerable to various pathogenic infections during its growth development. among different onion diseases, root and crown rot,caused by fusarium oxysporum f.sp. cepa, s considered an importantfungal disease. in this study, the inhibitory effect of bacillus cereus (isolates 22 and 52), b. subtilis (isolate 126), pseudomonas fluorescens (isolates 48 and chao), beno ... | 2004 | 15756854 |
| genetic diversity among bacillus anthracis, bacillus cereus and bacillus thuringiensis strains using repetitive element polymorphism-pcr. | repetitive element polymorphism-pcr (rep-pcr) is one of the tools that has been used to elucidate genetic diversity of related microorganisms. using the mb1 primer, rep-pcr fingerprints from 110 bacillus strains within the "b. cereus group" have identified eighteen distinct categories, while other more distantly related bacterial species fell within six additional categories. all bacillus anthracis strains tested were found to be monomorphic by fluorophore-enhanced rep-pcr (ferp) fingerprinting ... | 2004 | 15790070 |
| characteristics of bacterial strains inhabiting the wood of coniferous trees. | the presented studies embraced samples of wood chips from coniferous trees which contained layers of duramen, alburnum and bark. microbiological analysis involved qualitative and quantitative determination of bacterial flora inhabiting the studied wood material. the wood chips were found to contain primarily species belonging to the genera bacillus and pseudomonas. the presence of the potentially pathogenic species bacillus cereus 1, sphingomonas paucimobilis, aeromonas salmonicida and chryseomo ... | 2004 | 15790079 |
| temperature effect on bacterial growth rate: quantitative microbiology approach including cardinal values and variability estimates to perform growth simulations on/in food. | temperature effect on growth rates of listeria monocytogenes, salmonella, escherichia coli, clostridium perfringens and bacillus cereus, was studied. growth rates were obtained in laboratory medium by using a binary dilutions method in which 15 optical density curves were generated to determine one mu value. the temperature was in the range from 2 to 48 degrees c, depending on the bacterial species. data were analysed after a square root transformation. no large difference between the strains of ... | 2004 | 15854703 |
| a modified weibull model for bacterial inactivation. | in this paper, a modified weibull model is proposed to fit microbial survival curves. this model can incorporate shoulder and/or tailing phenomena if they are encountered. we aim to obtain an accurate fit of the "primary" modelling of the bacterial inactivation and to provide a useful and meaningful model for biologists and food industry. a delta parameter close to the classical concept of the d value, established for sterilisation processes, is used in the model. the specific parameterisation o ... | 2004 | 15854705 |
| structural and enzymatic analysis of soybean beta-amylase mutants with increased ph optimum. | comparison of the architecture around the active site of soybean beta-amylase and bacillus cereus beta-amylase showed that the hydrogen bond networks (glu380-(lys295-met51) and glu380-asn340-glu178) in soybean beta-amylase around the base catalytic residue, glu380, seem to contribute to the lower ph optimum of soybean beta-amylase. to convert the ph optimum of soybean beta-amylase (ph 5.4) to that of the bacterial type enzyme (ph 6.7), three mutants of soybean beta-amylase, m51t, e178y, and n340 ... | 2004 | 14638688 |
| multiple brain abscesses in an extremely preterm infant: treatment surveillance with interleukin-6 in the csf. | 2004 | 14586650 | |
| bacterial populations associated with meat from the deboning room of a high throughput red meat abattoir. | developing countries are faced with high incidences of food poisoning outbreaks, with obvious economic consequences. in highly perishable foodstuffs such as fresh red meat the threat of food poisoning is particularly intense. in this study, red meat samples were collected from a deboning room of a high throughput abattoir. the samples were analysed for the presence of bacillus cereus., staphylococcus aureus., pseudomonas spp., listeria monocytogenes., escherichia coli and salmonella spp. the aer ... | 2004 | 22060877 |