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the assessment of restrained eating. 20123457560
[successful chemotherapy in postoperative brain metastasis of breast cancer using acnu--two case reports].two cases of postoperative brain metastasis of breast cancer were evaluated after chemotherapy using acnu. case 1: a 47-year-old female, who had undergone right standard radical mastectomy in 1979 for breast cancer (t2 n0m0, papillo-tubular carcinoma), was treated with adm, tam, and 60co irradiation for bone metastasis in 1983. in 1984, she complained of loss of consciousness and paralysis of the extremities due to brain metastasis. after chemotherapy using acnu (100 mg x 3), brain metastasis co ...20123457552
brain thelohanellosis due to thelohanellus oculileucisci (myxozoa: myxosporea) in gobio gobio.the first case of a thelohanellus infection in the brain of gudgeons (gobio gobio) is described. the infection was found on three localities in southern bohemia, czechoslovakia. the spores measured 13.2-14.4 x 6.3-7.1 micron. the reasons are discussed for which the species was identified as t. oculileucisci trojan, 1909.20123428769
crystallographic structure and surface morphology of sintered carbonated apatites.densely sintered synthetic hydroxyapatite (ha) is used as an implant material because of its excellent tissue biocompatibility. in order to maximize the biological potential of this calcium phosphate, we have investigated the incorporation of carbonate into ha to make a material which more closely resembles the mineral found in bones and teeth. the aim of the present study was to determine the conditions under which sintered carbonated apatites of specific carbonate content could be produced. th ...20123410872
staff salaries and benefits: national and regional survey. 20123478249
[oral surgery in children's dentistry]. 20123478241
fracture healing perspectives. 20123595004
ovariectomy of adult mice exposed prenatally to diethylstilbestrol.mice exposed prenatally to diethylstilbestrol (des) were ovariectomized at 5-10 weeks of age, or at 15 months of age. they were maintained to old age and studied for incidence of des-related pathologic changes. uterine metaplasia and adenomyosis were not seen after early ovariectomy, but adenomyosis persisted after late ovariectomy. no adenocarcinomas of the genital tract were found in 30 mice ovariectomized early, or in 18 mice ovariectomized at 15 months of age. this result is significantly di ...20123815324
the process of risk management--and the pitfalls! 20123358330
lower abdominal and pelvic pain in women. a diagnostic approach. 20123355433
[clinical studies against chronic prostatitis and prostatitis-like syndrome (1). clinico-statistical observation].we clinically evaluated 605 cases of chronic prostatis and other resembling disorders. the incidence was 4.34% of all out-patients (7.54% of male patients). the average age was 38.1 years, and many were adolescents. a greater number of out-patients were seen in october, november, and december (p less than 0.01) and june (p less than 0.05). the type of disease was chronic prostatitis in 48.1% (bacterial in 5.3%, non-bacterial in 42.8%) and prostatitis-like syndrome in 51.9%. the incidence among m ...20123389292
[ratio and composition of the plasmalogen and diacylated forms of phospholipids in subcellular fractions of the avian brain].studies have been made on the specific content of plasmalogen and diacylated forms of phosphatidylethanolamine and phosphatidylcholine in subcellular fractions (myelin, nuclei, microsomes, mitochondria, synaptosomes) from the brain of pigeons, as well as in the myelin fraction from the brain of the crow corvus cornix and the hawk accipiter gentelis. fatty acid composition and fatty aldehyde composition of these two main phospholipids of the brain were studied in the subcellular fractions obtaine ...20123376631
the deprofessionalization of medicine. 20123352114
psychiatric disorders in pediatric primary care. prevalence and risk factors.children aged 7 to 11 years visiting their primary care pediatrician for a wide range of reasons were studied to determine the one-year prevalence of dsm-iii disorders and the risk factors associated with them. parents completing the child behavior checklist about their children identified problems that placed 24.7% of 789 children in the clinical range. detailed psychiatric interviews with 300 parents and children, using the diagnostic interview schedule for children, yielded a one-year weighte ...20123264146
[the significance of early coronary reperfusion in patients with acute myocardial infarction assessed by regional wall motion].left ventricular ejection fraction, regional wall motion, hospital mortality rate, and reocclusion rate of the infarct-related coronary artery after thrombolytic therapy were examined in 164 consecutive patients who were admitted within 12 hours of the onsets of their symptoms of acute myocardial infarction. the patients were divided into three groups based on the findings of initial coronary angiography before and after administration of urokinase: (1) stenosed (the infarct-related coronary blo ...20123267729
[myelography and computerized tomography in the diagnosis of spinal cord compression in children]. 20123302968
[studies on the isolation and structures of baohuoside-ii, iii, iv and v]. 20123256201
taking a baby. 20123337152
beat-to-beat blood pressure response in asymptomatic iddm subjects.nine insulin-dependent diabetic (iddm) patients (aged 25-37 yr) with no symptoms of autonomic neuropathy and 15 healthy control subjects (aged 26-39 yr) were studied at rest and during tests of valsalva maneuver, deep breathing, cold pressor, and postural change from sitting to standing. continuous (beat-to-beat) measures were taken of heart rate, systolic blood pressure, diastolic blood pressure, and skin conductance. the diabetic patients were differentiated from the control group by the follo ...20123246197
the pyv virulence plasmids of yersinia pseudotuberculosis and y. pestis contain a conserved dna region responsible for the mobilization by the self-transmissible plasmid pye854.pathogenic yersinia strains possess a 70 kb virulence plasmid which can be transmitted to other strains by conjugation systems expressed by co-resident plasmids. we isolated a 720 bp fragment of the yersinia pseudotuberculosis virulence plasmid p1340 that mediated mobilization of the vector piv2 by the self-transmissible plasmid pye854. the p1340 mobilization region contains the resolvase gene tnpr and its proposed resolution site res. both elements are required for mobilization. plasmid transfe ...201223760829
acute encephalopathy and tubulointerstitial nephritis associated with yersinia pseudotuberculosis.we report the case of a 28-month-old boy with encephalopathy and acute tubulointerstitial nephritis possibly associated with yersinia pseudotuberculosis (yp) infection. he was transferred to our center because of impairment of renal function and altered consciousness. he had fever for 5 days after recurrent vomiting and diarrhea. computed tomography scan was normal, but electroencephalogram (eeg) analyses showed generalized slow wave patterns. continuous hemodialysis was undergone and then his r ...201223279023
structure and function of cytidine monophosphate kinase from yersinia pseudotuberculosis, essential for virulence but not for survival.the need for new antibiotics has become pressing in light of the emergence of antibiotic-resistant strains of human pathogens. yersinia pestis, the causative agent of plague, is a public health threat and also an agent of concern in biodefence. it is a recently emerged clonal derivative of the enteric pathogen yersinia pseudotuberculosis. previously, we developed a bioinformatic approach to identify proteins that may be suitable targets for antimicrobial therapy and in particular for the treatme ...201223271832
nlrc4 inflammasomes in dendritic cells regulate noncognate effector function by memory cd8(+) t cells.memory t cells exert antigen-independent effector functions, but how these responses are regulated is unclear. we discovered an in vivo link between flagellin-induced nlrc4 inflammasome activation in splenic dendritic cells (dcs) and host protective interferon-γ (ifn-γ) secretion by noncognate memory cd8(+) t cells, which could be activated by salmonella enterica serovar typhimurium, yersinia pseudotuberculosis and pseudomonas aeruginosa. we show that cd8α(+) dcs were particularly efficient at s ...201222231517
a protective epitope in type iii effector yope is a major cd8 t cell antigen during primary infection with yersinia pseudotuberculosis.virulence in human-pathogenic yersinia species is associated with a plasmid-encoded type iii secretion system that translocates a set of yop effector proteins into host cells. one effector, yope, functions as a rho gtpase-activating protein (gap). in addition to acting as a virulence factor, yope can function as a protective antigen. c57bl/6 mice infected with attenuated yersinia pestis generate a dominant h2-k(b)-restricted cd8 t cell response to an epitope in the n-terminal domain of yope (yop ...201222064714
the outer membrane protein a (ompa) of yersinia pestis promotes intracellular survival and virulence in mice.the plague bacterium yersinia pestis has a number of well-described strategies to protect itself from both host cells and soluble factors. in an effort to identify additional anti-host factors, we employed a transposon site hybridization (trash)-based approach to screen 10(5)y. pestis mutants in an in vitro infection system. in addition to loci encoding various components of the well-characterized type iii secretion system (t3ss), our screen unambiguously identified ompa as a pro-survival gene. ...201222023991
haemophilia-associated yersinia pseudotuberculosis serotype o:1 septicaemia: the role of iron.septicaemia and septic arthritis due to yersinia pseudotuberculosis are rare diseases with high mortality rates. reactive arthritis caused by yersinia infection is a well-known complication but septic arthritis is found at a much lower frequency. it has already been established that there is a relationship between yersiniosis and iron but there are currently no data about yersiniosis and haematological disorders such as haemophilia. we report a case of septic arthritis due to y. pseudotuberculos ...201222184209
rearrest following residential treatment for repeat offender drunken drivers.multiple offenders are at high risk for continued drunken driving. massachusetts, therefore, mandated that individuals convicted of a second drunken driving offense either be committed for a minimum of 7 days in a house of correction or enter a 14-day residential alcoholism treatment program for second offenders. a 2-year follow-up study of arrest rates assessed the impact of the two sentencing options on subsequent arrests for driving under the influence of liquor (duil). the incarcerated sampl ...20123347067
[immunogenetic analysis of autoantibody production induced by a b cell differentiation factor, b151-trf2]. 20123267118
restless legs syndrome.patients with restless legs syndrome often have difficulty describing their symptoms. three features characterize the classic syndrome: an unusual, almost indescribable sensation in the lower extremities; an irresistible urge to move the legs, and the exclusive occurrence of the symptoms at rest, with their relief by movement. restless legs syndrome may cause severe insomnia. variants of the syndrome, as well as numerous associated conditions, have been described. the etiology is unknown. clonaz ...20123510520
juvenile recurrent parotitis: clinicoradiologic follow-up study and the beneficial effect of sialography.in twenty-two children juvenile recurrent parotitis (jrp) was diagnosed on the basis of age at onset, duration of parotid swelling episodes, frequency of attacks, lack of pus formation, and typical sialograms. age at onset and severity of the disease varied widely. there was a high incidence of upper respiratory tract infection, and in some cases a familial history of jrp was noted. the sialograms revealed acinar and ductal atrophy and severe sialectasis. a dominant feature was impaired glandula ...20123459982
incontinence and the health visitor. 20123372255
the professional ethics and practice committee: a step toward the achievement of excellence. 20123412661
abnormal glycosylation of serum igg from patients with chronic inflammatory diseases.results of carbohydrate analysis of serum igg from patients with rheumatoid arthritis (ra) confirmed an earlier report that igg from patients with ra is galactosylated to a lesser extent than igg from healthy individuals. in contrast to the previous report, we found that the content of galactose in igg from controls and ra patients was negatively correlated with age (p = 0.026 and p = 0.010, respectively). in ra patients, the igg content of galactose was also negatively correlated with the pain ...20123358797
third generation cephalosporin antibiotics in surgical practice.the new third generation cephalosporins have a significantly greater spectrum of action against gram-negative bacteria likely to be encountered in surgical infections. this expanded spectrum may permit these drugs to be used in place of combination therapy in patients with polymicrobial infections; however, current evidence does not indicate superior results with these agents over earlier generation choices for surgical prophylaxis. the toxicity profile of these drugs warrants close monitoring f ...20123511763
disturbances in object representation. 20123438374
misidentification of urine lipid bodies owing to use of starch-powdered gloves. 20123416464
toxicity of diethyltoluamide-containing insect repellents. 20123352115
[application of professor machtou's compactors]. 20123482391
cancer, stress and personality: a correlational investigation of life-events, repression-sensitization and locus of control.this study investigated the hypothesis that stress-inducing life experiences and the personality factors of external locus of control and tendency to repress emotion are relevant in the aetiology of cancer. fifty cancer patients scored higher than 50 matched controls on a life-events scale (especially negative events), but were found to be lower on repression of emotion. there was no difference between groups on locus of control.20123401426
avascular necrosis of the proximal carpal row. a case report.avascular necrosis of bone has been described in many sites, including the carpal bones. the case history of a patient with avascular necrosis of the entire proximal row, previously undescribed, is detailed. numerous contributing factors, including steroid administration, hyperlipidemia, and systemic lupus erythematosus are noted. occult wrist pain in any patient with known risk factors for avascular necrosis is a warning sign; early bone scan may help prevent late complications.20123426328
patterns of recurrence of esophageal carcinoma after transhiatal esophagectomy and gastric interposition.serial barium and ct studies were performed for follow-up of 35 patients who had undergone transhiatal esophagectomy with gastric interposition for esophageal carcinoma. the results were compared with the clinical and pathologic findings. thirteen patients (37%) were clinically and radiologically free of tumor recurrence after a mean observation period of 18 months. twenty-one patients (60%) developed recurrent carcinoma within 12 months and one patient (3%) within 14 months. thirteen patients w ...20123492103
conformation of methylated amino acids: structure of 3,4-dimethoxy-alpha-methyl-dl-phenylalanine sesquihydrate.c12h17no4.1.5h2o, mr = 266.3, triclinic, p1, a = 5.872 (1), b = 11.437 (2), c = 20.434 (1) a, alpha = 95.74 (1), beta = 96.91 (1), gamma = 89.18 (1) degrees, v = 1355.5 a3, z = 4, dm = 1.29, d chi = 1.305 g cm-3, lambda(cu k alpha) = 1.5418 a, mu = 8.3 cm-1, f(000) = 572, t = 294 k, r = 0.038 for 4006 reflections, i greater than or equal to 3 sigma(i). both the molecules a and b in the asymmetric unit exist as zwitterions. with respect to the d enantiomer, the torsion angles psi 1 and psi 2 are ...20123271079
atherosclerosis precursors in schoolchildren--results of a two-year intervention study.in order to reduce precursors of atherosclerosis, an intervention trial was carried out in pupils aged 10-11 years at the time of inclusion into the study (5th graders). the main goal of the study was to encourage health-promoting behaviour patterns--mostly present in the schoolchildren at that time--such as non-smoking, a negative attitude toward future smoking, a high level of physical activity including sports. the study design included dietary measures aimed at lowering blood pressure and to ...20123436146
maternal antibodies to paternal b-lymphocytes in normal and abnormal pregnancy.sera were obtained prior to conception and during the first trimester of subsequent pregnancies from 22 women over 27 pregnancies; on 15 occasions these pregnancies ended in spontaneous abortion, whereas the remaining 12 achieved live normal babies. in only one pregnancy ending in abortion could antibodies directed to paternal b-lymphocytes determined by the ea rosette inhibition (eai) assay be detected in the mother's serum, whereas five of the 12 successful pregnancies were associated with suc ...20123485929
isolation of fractions rich in y sperm by means of differential motility using hen's egg yolk as the medium: a preliminary report. 20123418265
'treatment ind' is an oxymoron. 20123352116
redressing the balance. 20123292931
[mercury content of the pulp in unfilled and amalgam-filled teeth]. 20123483683
managing both members of a two-pharmacist couple: the employer's perspective.employer considerations in hiring and managing the two-pharmacist couple are discussed. in recruiting one member of a two-pharmacist couple, an employer may need to consider hiring the other spouse, especially if the employment requires relocation and if the institution is the only practice site in the area. managers should be aware of potential difficulties associated with this practice, such as the potential for formal charges if illegal discrimination is perceived as a motive for selecting a ...20123434583
artifacts and diagnostic pitfalls on magnetic resonance imaging: a clinical review.high field mri of the brain occasionally exhibits imaging artifacts; most artifacts are obvious and easily recognized, but some are subtle and mimic disease. a thorough understanding of brain mri artifacts is important to avoid potential diagnostic pitfalls. some imaging techniques or procedures could be utilized to remove or identify artifacts. these include additional projections, different pulse sequence, and 90 degree shift of phase-encoding gradient. the use of respiratory gating or cardiac ...20123380943
case records of the massachusetts general hospital. weekly clinicopathological exercises. case 38-1987. a 22-year-old woman with a pulmonary mass and lesions in the bones and brain. 20123627186
how to develop tomorrow's nursing leaders.a pivotal event for nursing was the "international encounter on leadership in nursing for health for all" in tokyo, japan, in april 1986. here, nursing leaders from every corner of the globe exchanged views and were spurred on to create a movement to achieve who's "health for all" goal. finally, after a long wait for permission to print, inr offers its readers one such view given at the conference. this paper gives not only advice on how to prepare tomorrow's nursing leaders in hfa but also an a ...20123417396
crp induces switching of the csrb and csrc rnas in yersinia pseudotuberculosis and links nutritional status to virulence.colonization of the intestinal tract and dissemination into deeper tissues by the enteric pathogen yersinia pseudotuberculosis demands expression of a special set of virulence factors important for the initiation and the persistence of the infection. in this study we demonstrate that many virulence-associated functions are coregulated with the carbohydrate metabolism. this link is mediated by the carbon storage regulator (csr) system, including the regulatory rnas csrb and csrc, and the camp rec ...201223251905
yopj-induced caspase-1 activation in yersinia-infected macrophages: independent of apoptosis, linked to necrosis, dispensable for innate host defense.yersinia outer protein j (yopj) is a type iii secretion system (t3ss) effector of pathogenic yersinia (yersinia pestis, yersinia enterocolitica and yersinia pseudotuberculosis) that is secreted into host cells. yopj inhibits survival response pathways in macrophages, causing cell death. allelic variation of yopj is responsible for differential cytotoxicity in yersinia strains. yopj isoforms in y. enterocolitica o:8 (yopp) and y. pestis kim (yopj(kim)) strains have high cytotoxic activity. in add ...201222563435
a conjugative 38 kb plasmid is present in multiple subspecies of xylella fastidiosa.a ≈ 38kb plasmid (pxf-riv5) was present in the riv5 strain of xylella fastidiosa subsp. multiplex isolated from ornamental plum in southern california. the complete nucleotide sequence of pxf-riv5 is almost identical to that of pxfas01 from x. fastidiosa subsp. fastidiosa strain m23; the two plasmids vary at only 6 nucleotide positions. blast searches and phylogenetic analyses indicate pxf-riv5 and pxfas01 share some similarity to chromosomal and plasmid (pxf51) sequences of x. fastidiosa subsp. ...201223251694
yersinia infection tools-characterization of structure and function of adhesins.among the seventeen species of the gram-negative genus yersinia, three have been shown to be virulent and pathogenic to humans and animals-y. enterocolitica, y. pseudotuberculosis, and y. pestis. in order to be so, they are armoured with various factors that help them adhere to tissues and organelles, cross the cellular barrier and escape the immune system during host invasion. the group of proteins that mediate pathogen-host interactions constitute adhesins. invasin, ail, yada, yadb, yadc, pla, ...201223316485
interactions of the cpxa sensor kinase and cognate cpxr response regulator from yersinia pseudotuberculosis.the cpxa sensor kinase-cpxr response regulator two-component regulatory system is a sentinel of bacterial envelope integrity. integrating diverse signals, it can alter the expression of a wide array of components that serve to shield the envelope from damage and to promote bacterial survival. in bacterial pathogens such as yersinia pseudotuberculosis, this also extends to pathogenesis. cpxr is thought to dimerize upon phosphorylation by the sensor kinase cpxa. this phosphorylation enables cpxr b ...201223013530
evaluation of a single procedure allowing the isolation of enteropathogenic yersinia along with other bacterial enteropathogens from human stools.enteropathogenic yersinia are among the most frequent agents of human diarrhea in temperate and cold countries. however, the incidence of yersiniosis is largely underestimated because of the peculiar growth characteristics of pathogenic yersinia, which make their isolation from poly-contaminated samples difficult. the use of specific procedures for yersinia isolation is required, but is expensive and time consuming, and therefore is not systematically performed in clinical pathology laboratories ...201222911756
invasive escherichia coli vaccines expressing brucella melitensis outer membrane proteins 31 or 16 or periplasmic protein bp26 confer protection in mice challenged with b. melitensis.because of the serious economic and medical consequences of brucellosis, efforts are to prevent infection of domestic animals through vaccines. many disadvantages are associated with the current brucella melitensis rev.1 vaccine prompting development of alternative vaccines and delivery. escherichia coli (dh5α) was engineered to express a plasmid containing the inv gene from yersinia pseudotuberculosis and the hly gene from listeria monocytogenes. these recombinant invasive e. coli expressing b. ...201222546330
abortion in small ruminants in the netherlands between 2006 and 2011.during five successive lambing seasons between 2006 and 2011, 453 submissions of abortion material, 282 of ovine and 171 of caprine origin, were examined at the animal health service in the netherlands. infectious agents as the most plausible cause of the abortion were found in 48 percent of the ovine submissions and in 34 percent of the caprine submissions. submission of both aborted fetus and placental membranes increased the diagnostic yield of laboratory investigations (17 percent and 21 per ...201222900421
derivatives of 8-hydroxyquinoline--antibacterial agents that target intra- and extracellular gram-negative pathogens.small molecule screening identified 5-nitro-7-((4-phenylpiperazine-1-yl-)methyl)quinolin-8-ol inp1750 as a putative inhibitor of type iii secretion (t3s) in the gram-negative pathogen yersinia pseudotuberculosis. in this study we report structure-activity relationships for inhibition of t3s and show that the most potent compounds target both the extracellular bacterium y. pseudotuberculosis and the intracellular pathogen chlamydia trachomatis in cell-based infection models.201222525317
cluster analysis of host cytokine responses to biodefense pathogens in a whole blood ex vivo exposure model (weem).rapid detection and therapeutic intervention for infectious and emerging diseases is a major scientific goal in biodefense and public health. toward this end, cytokine profiles in human blood were investigated using a human whole blood ex vivo exposure model, called weem.201222607329
design and characterization of novel hybrid antimicrobial peptides based on cecropin a, ll-37 and magainin ii.antimicrobial peptides (amps) are a naturally occurring component of the innate immune response of many organisms and can have activity against both gram-negative and gram-positive bacterial species. in order to optimize and improve the direct antimicrobial effect of amps against a broad spectrum of bacterial species, novel synthetic hybrids were rationally designed from cecropin a, ll-37 and magainin ii. amps were selected based on their α-helical secondary structure and fragments of these were ...201222289499
molecular cloning, isolation, and properties of chaperone skp from yersinia pseudotuberculosis.the skp gene of yersinia pseudotuberculosis was expressed without its signal sequence in escherichia coli bl21(de3) cells. the recombinant protein skp accumulated in soluble form in the cytoplasm of the producer strain. the protein was isolated and characterized: the molecular weight, isoelectric point, n-terminal amino acid sequence (20 amino acid residues), and the content of the secondary structure elements were determined. using cross-linking stabilization and high-mass maldi-tof mass spectr ...201223240570
the yersinia pseudotuberculosis degradosome is required for oxidative stress, while its pnpase subunit plays a degradosome-independent role in cold growth.yersinia polynucleotide phosphorylase (pnpase), a 3'-5' exoribonuclease, has been shown to affect growth during several stress responses. in escherichia coli, pnpase is one of the subunits of a multiprotein complex known as the degradosome, but also has degradosome-independent functions. the carboxy-terminus of e. coli ribonuclease e (rnase e) serves as the scaffold upon which pnpase, enolase (a glycolytic enzyme), and rhlb helicase all have been shown to bind. in the yersiniae, only pnpase has ...201223082859
the yersinia pseudotuberculosis outer membrane protein ail recruits the human complement regulatory protein factor h.previous investigations characterizing the mechanism(s) of complement resistance in yersinia pseudotuberculosis showed that the outer membrane protein ail can functionally recruit the regulator of the classical and lectin pathways of complement, c4b-binding protein. in this study, we extend these observations and show that ail can also recruit the regulator of the alternative pathway (ap), factor h (fh). binding to fh was dependent on ail expression and observed in the context of full-length lps ...201222956584
[yersinia pseudotuberculosis mutant ompf porins with deletions of the external loops: genetic constructions design, expression, isolation and refolding].yersinia pseudotuberculosis outer membrane (om) recombinant mutant ompf porins with deletions of the external loops l1, l6 and l8 were obtained using site-directed mutagenesis of the recombinant plasmid including ompf gene. heterologeous expression of the mutant proteins was carried out in strain rosetta of escherichia coli (novagen, usa), porins with the deletions were isolated from the inclusion bodies. mutant proteins in oligomeric form were obtained as result of dialysis and ion-exchange chr ...201222792719
bacteriophages capable of lysing yersinia pestis and yersinia pseudotuberculosis: efficiency of plating tests and identification of receptors in escherichia coli k-12. 201222782755
fdec, a novel broadly conserved escherichia coli adhesin eliciting protection against urinary tract infections.the increasing antibiotic resistance of pathogenic escherichia coli species and the absence of a pan-protective vaccine pose major health concerns. we recently identified, by subtractive reverse vaccinology, nine escherichia coli antigens that protect mice from sepsis. in this study, we characterized one of them, ecok1_0290, named fdec (factor adherence e. coli) for its ability to mediate e. coli adhesion to mammalian cells and extracellular matrix. this adhesive propensity was consistent with t ...201222496310
functional recruitment of the human complement inhibitor c4bp to yersinia pseudotuberculosis outer membrane protein ail.ail is a 17-kda chromosomally encoded outer membrane protein that mediates serum resistance (complement resistance) in the pathogenic yersiniae (yersinia pestis, y. enterocolitica, and y. pseudotuberculosis). in this article, we demonstrate that y. pseudotuberculosis ail from strains pb1, 2812/79, and ypiii/pib1 (serotypes o:1a, o:1b, and o:3, respectively) can bind the inhibitor of the classical and lectin pathways of complement, c4b-binding protein (c4bp). binding was observed irrespective of ...201222467648
a natural system of chromosome transfer in yersinia pseudotuberculosis.the high pathogenicity island of yersinia pseudotuberculosis ip32637 was previously shown to be horizontally transferable as part of a large chromosomal segment. we demonstrate here that at low temperature other chromosomal loci, as well as a non-mobilizable plasmid (puc4k), are also transferable. this transfer, designated gdt4 (generalized dna transfer at 4°c), required the presence of an ip32637 endogenous plasmid (pgdt4) that carries several mobile genetic elements and a conjugation machinery ...201222412380
toxin-induced rhoa activity mediates ccl1-triggered signal transducers and activators of transcription protein signaling.rhoa is reportedly involved in signal transducers and activators of transcription (stat)-dependent transcription. however, the pathway connecting the gtpase and stat signaling has not been characterized. here, we made use of bacterial toxins, which directly activate rho gtpases to analyze this pathway. cytotoxic necrotizing factors (cnfs) are produced by pathogenic escherichia coli strains and by yersinia pseudotuberculosis. they activate small gtpases of the rho family by deamidation of a gluta ...201222311973
surface display of n-terminally anchored invasin by lactobacillus plantarum activates nf-κb in monocytes.the probiotic lactic acid bacterium lactobacillus plantarum is a potential delivery vehicle for mucosal vaccines because of its generally regarded as safe (gras) status and ability to persist at the mucosal surfaces of the human intestine. however, the inherent immunogenicity of vaccine antigens is in many cases insufficient to elicit an efficient immune response, implying that additional adjuvants are needed to enhance the antigen immunogenicity. the goal of the present study was to increase th ...201222706054
pupil diameter in migraine and tension headache.pupil diameter was measured in darkness and in dull and bright illumination in 39 migrainous patients and in 15 tension headache sufferers during headache. in 21 migrainous patients, measurements were repeated during the headache-free interval. mean pupil diameter was smaller in patients with common migraine at the time of examination than in 20 nonheadache control subjects, and smaller on the symptomatic side in migrainous patients with unilateral headache. during the headache-free interval mea ...20123572438
failure of nonstress test and doppler-assessed umbilical arterial blood flow to detect imminent intrauterine death.an unexplained intrauterine death occurred within 12 hours of a normal reactive nonstress test. furthermore the umbilical arterial impedance measured by pulsed doppler ultrasound was also normal.20123518462
failure of nonstress test and doppler-assessed umbilical arterial blood flow to detect imminent intrauterine death.an unexplained intrauterine death occurred within 12 hours of a normal reactive nonstress test. furthermore the umbilical arterial impedance measured by pulsed doppler ultrasound was also normal.20123518462
is the role of basic amino terminal arm replaced by polyamines in tymoviruses? 20123255672
central nervous system lesions and advanced ovarian cancer.over the past 5 years we have seen seven cases of central nervous system tumors in patients with advanced or recurrent ovarian carcinoma. these have ranged in location from the conus medullaris to the brain and have varied in time of occurrence from the time of cancer diagnosis to 63 months following surgery. a diagnosis of metastatic ovarian carcinoma was established in six of the cases, while one patient had a second primary malignancy. all cases had prodromal and localizing neurologic symptom ...20123366397
expressed recombinant cadherins mediate cell sorting in model systems.cadherins are cell-surface glycoproteins responsible for ca2+-dependent cell-to-cell adhesion. e- or p-cadherin was transfected into l cells, which normally have little cadherin activity, and cellular aggregation of the resulting transfectants was observed to be a function of the cadherin molecule expressed. transfected cells preferentially adhered to cells expressing the same cadherin subclass. furthermore, in reconstituted embryonic lung tissue, e-cadherin-expressing l cells were associated wi ...20123416359
electrophoretic mobility, concentration, and activity of alpha 1-antitrypsin in serum of patients undergoing bone-marrow transplantation.we have observed an electrophoretically abnormal, nonfunctional species of alpha 1-antitrypsin in serum from patients who were receiving bone-marrow transplants for treatment of leukemia or aplastic anemia. three of four patients in whose serum this protein appeared died soon after; the fourth recovered, and the disappearance of the abnormal alpha 1-antitrypsin paralleled his recovery. this suggests that the inability to maintain functional activity of alpha 1-antitrypsin predisposes patients to ...20123542290
research in home care.past and present research in the home care setting predicts future trends. home care research studies will continue to reflect social and economic issues of the future since research and researchers do not exist in a vacuum but are influenced by their era. because of the generalized concern about economic stability and health care costs and the heightened interest in home care, investigations related to nursing interventions, client outcomes, and reimbursement will increase. as researchers searc ...20123368398
interleukin-10 induction is an important virulence function of the yersinia pseudotuberculosis type iii effector yopm.pathogenic yersinia species modulate host immune responses through the activity of a plasmid-encoded type iii secretion system and its associated effector proteins. one effector, yopm, is a leucine-rich-repeat-containing protein that is important for virulence in murine models of yersinia infection. although the mechanism by which yopm promotes virulence is unknown, we previously demonstrated that yopm was required for the induction of high levels of the immunosuppressive cytokine interleukin-10 ...201222547545
quantification of bacterial indicators and zoonotic pathogens in dairy wastewater ponds.zoonotic pathogens in land-applied dairy wastewaters are a potential health risk. the occurrence and abundance of 10 pathogens and 3 fecal indicators were determined by quantitative real-time pcr (qpcr) in samples from 30 dairy wastewaters from southern idaho. samples tested positive for campylobacter jejuni, stx(1)- and eaea-positive escherichia coli, listeria monocytogenes, mycobacterium avium subsp. paratuberculosis, and salmonella enterica, with mean recoveries of genomic dna corresponding t ...201222983964
novel genomic tools for specific and real-time detection of biothreat and frequently encountered foodborne pathogens.the bacterial genera escherichia, salmonella, shigella, vibrio, yersinia, and francisella include important food safety and biothreat agents. by extensive mining of the whole genome and protein databases of diverse, closely and distantly related bacterial species and strains, we have identified novel genome regions, which we utilized to develop a rapid detection platform for these pathogens. the specific genomic targets we have identified to design the primers in francisella tularensis subsp. tu ...201222488053
yersinia--flea interactions and the evolution of the arthropod-borne transmission route of plague.yersinia pestis, the causative agent of plague, is unique among the enteric group of gram-negative bacteria in relying on a blood-feeding insect for transmission. the yersinia-flea interactions that enable plague transmission cycles have had profound historical consequences as manifested by human plague pandemics. the arthropod-borne transmission route was a radical ecologic change from the food-borne and water-borne transmission route of yersinia pseudotuberculosis, from which y. pestis diverge ...201222406208
plague in the genomic area.with plague being not only a subject of interest for historians, but still a disease of public health concern in several countries, mainly in africa, there were hopes that analyses of the yersinia pestis genomes would put an end to this deadly epidemic pathogen. genomics revealed that y. pestis isolates evolved from yersinia pseudotuberculosis in central asia some millennia ago, after the acquisition of two y. pestis-specific plasmids balanced genomic reduction parallel with the expansion of ins ...201222369155
yersinia pestis lineages in mongolia.whole genome sequencing allowed the development of a number of high resolution sequence based typing tools for yersinia (y.) pestis. the application of these methods on isolates from most known foci worldwide and in particular from china and the former soviet union has dramatically improved our understanding of the population structure of this species. in the current view, y. pestis including the non or moderate human pathogen y. pestis subspecies microtus emerged from yersinia pseudotuberculosi ...201222363455
an encapsulated yersinia pseudotuberculosis is a highly efficient vaccine against pneumonic plague.plague is still a public health problem in the world and is re-emerging, but no efficient vaccine is available. we previously reported that oral inoculation of a live attenuated yersinia pseudotuberculosis, the recent ancestor of yersinia pestis, provided protection against bubonic plague. however, the strain poorly protected against pneumonic plague, the most deadly and contagious form of the disease, and was not genetically defined.201222348169
pulmonary infection by yersinia pestis rapidly establishes a permissive environment for microbial proliferation.disease progression of primary pneumonic plague is biphasic, consisting of a preinflammatory and a proinflammatory phase. during the long preinflammatory phase, bacteria replicate to high levels, seemingly uninhibited by normal pulmonary defenses. in a coinfection model of pneumonic plague, it appears that yersinia pestis quickly creates a localized, dominant anti-inflammatory state that allows for the survival and rapid growth of both itself and normally avirulent organisms. yersinia pseudotube ...201222308352
comparative genomic analysis of gene variations of two chinese yersinia pestis isolates from vaccine strain ev76.to investigate genomic variations of two chinese yersinia pestis isolates that were isolated from different plague foci obtained from vaccine strain ev76 from the yunnan province of china.201223026524
modern advances against plague.plague has been a scourge of humanity, responsible for the deaths of millions. the etiological agent, yersinia pestis, has evolved relatively recently from an enteropathogen, yersinia pseudotuberculosis. the evolution of the plague pathogen has involved a complex series of genetic acquisitions, deletions, and rearrangements in its transition from an enteric niche to becoming a systemic, flea-vectored pathogen. with the advent of modern molecular biology techniques, we are starting to understand ...201222958531
yersinia pestis versus yersinia pseudotuberculosis: effects on host macrophages.yersinia pestis, the causative agent of plague, is proved to be a recently emerged clone from y. pseudotuberculosis. however, the diseases they cause and their patterns of transmission are very different. people always focus on the genetic changes between y. pestis and y. pseudotuberculosis to reveal their pathogenic differences, and little is known about host defence differences to these two yersinia. in this study, the effects of y. pestis and y. pseudotuberculosis on macrophages were analysed ...201222882408
evolution and virulence contributions of the autotransporter proteins yapj and yapk of yersinia pestis co92 and their homologs in y. pseudotuberculosis ip32953.yersinia pestis, the causative agent of plague, evolved from the gastrointestinal pathogen yersinia pseudotuberculosis. both species have numerous type va autotransporters, most of which appear to be highly conserved. in y. pestis co92, the autotransporter genes yapk and yapj share a high level of sequence identity. by comparing yapk and yapj to three homologous genes in y. pseudotuberculosis ip32953 (yptb0365, yptb3285, and yptb3286), we show that yapk is conserved in y. pseudotuberculosis, whi ...201222802344
assignment and secondary structure of the yada membrane protein by solid-state mas nmr.we report the complete solid-state mas nmr resonance assignment of a medium-sized, trimeric membrane protein, yada-m. the protein yada (yersinia adhesin a) is an important virulence factor of enteropathogenic yersinia species (such as yersinia enterocolitica and yersinia pseudotuberculosis). yada is localized on the bacterial cell surface and is involved in adhesion to host cells and tissues. it is anchored in the outer membrane by a transmembrane anchor domain (yada-m). this domain hosts the so ...201223150774
detection and isolation of yersinia pestis without fraction 1 antigen by monoclonal antibody in foods and water.most available immunoassays for yersinia pestis are based on the detection of fraction 1 antigen (f1) when yersiniae are grown at 37°c. a monoclonal antibody (mab) was developed based on the detection of surface antigens that are not f1. f1-deficient y. pestis cells were induced and used to immunize balb/c mice from which mab (immunoglobulin g1), which specifically recognizes y. pestis, with or without f1, was obtained. this mab (6b5) did not cross-react with enteric bacteria, including yersinia ...201222947461
growth of yersinia enterocolitica and y. pseudotuberculosis in yersinia selective enrichment broth according to ossmer.the growth of yersinia enterocolitica and yersinia pseudotuberculosis in yersinia enrichment broth according to ossmer (yseo) was investigated. y. enterocolitica reached a higher concentration than y. pseudotuberculosis but both always exceeded 10(6)cfu/ml. the medium may be useful for the detection of both species in foods.201222450139
establishment of a health surveillance program for reintroduction of the eurasian beaver (castor fiber) into scotland.in 2009 and 2010 16 norwegian eurasian beavers (castor fiber) were reintroduced to knapdale, scotland as part of a 5-yr reintroduction trial (scottish beaver trial). despite numerous reintroduction programs throughout europe there is no published information concerning recommended health surveillance during beaver reintroduction and only one publication describing causes of mortality. we describe the establishment of a health surveillance program based on international union of conservation of n ...201223060498
a novel high-resolution melting analysis-based method for yersinia pseudotuberculosis genotyping.yersinia pseudotuberculosis is an enteric pathogen that is environmentally widespread and is known to cause human and animal infections. the development of a fast and inexpensive typing system is necessary to facilitate epidemiological studies of y. pseudotuberculosis infections. in this study, we aimed to develop a method of y. pseudotuberculosis genotyping based on determining differences in single-nucleotide polymorphisms (snps) using a high-resolution melting analysis (hrma). using a set of ...201223041267
small molecule screening for inhibitors of the yoph phosphatase of yersinia pseudotuberculosis. 201222782782
regulation of virulence gene expression by regulatory rna elements in yersinia pseudotuberculosis. 201222782778
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