Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| a novel pcr-based approach for accurate identification of vibrio parahaemolyticus. | a pcr-based assay was developed for more accurate identification of vibrio parahaemolyticus through targeting the bla carb-17 like element, an intrinsic β-lactamase gene that may also be regarded as a novel species-specific genetic marker of this organism. homologous analysis showed that bla carb-17 like genes were more conservative than the tlh, toxr and atpa genes, the genetic markers commonly used as detection targets in identification of v. parahaemolyticus. our data showed that this bla car ... | 2016 | 26858713 |
| the salmonella in silico typing resource (sistr): an open web-accessible tool for rapidly typing and subtyping draft salmonella genome assemblies. | for nearly 100 years serotyping has been the gold standard for the identification of salmonella serovars. despite the increasing adoption of dna-based subtyping approaches, serotype information remains a cornerstone in food safety and public health activities aimed at reducing the burden of salmonellosis. at the same time, recent advances in whole-genome sequencing (wgs) promise to revolutionize our ability to perform advanced pathogen characterization in support of improved source attribution a ... | 2016 | 26800248 |
| diagnostic methods of helicobacter pylori infection for epidemiological studies: critical importance of indirect test validation. | among the methods developed to detect h. pylori infection, determining the gold standard remains debatable, especially for epidemiological studies. due to the decreasing sensitivity of direct diagnostic tests (histopathology and/or immunohistochemistry [ihc], rapid urease test [rut], and culture), several indirect tests, including antibody-based tests (serology and urine test), urea breath test (ubt), and stool antigen test (sat) have been developed to diagnose h. pylori infection. among the ind ... | 2016 | 26904678 |
| a novel 7-single nucleotide polymorphism-based clonotyping test allows rapid prediction of antimicrobial susceptibility of extraintestinal escherichia coli directly from urine specimens. | background. escherichia coli is a highly clonal pathogen. extraintestinal isolates belong to a limited number of genetically related groups, which often exhibit characteristic antimicrobial resistance profiles. methods. we developed a rapid clonotyping method for extraintestinal e coli based on detection of the presence or absence of 7 single nucleotide polymorphisms (snps) within 2 genes (fumc and fimh). a reference set of 2559 e coli isolates, primarily of urinary origin, was used to predict ... | 2016 | 26925427 |
| mechanism of the appabluf photocycle probed by site-specific incorporation of fluorotyrosine residues: effect of the y21 pka on the forward and reverse ground-state reactions. | the transcriptional antirepressor appa is a blue light using flavin (bluf) photoreceptor that releases the transcriptional repressor ppsr upon photoexcitation. light activation of appa involves changes in a hydrogen-bonding network that surrounds the flavin chromophore on the nanosecond time scale, while the dark state of appa is then recovered in a light-independent reaction with a dramatically longer half-life of 15 min. residue y21, a component of the hydrogen-bonding network, is known to be ... | 2016 | 26708408 |
| significance and roles of proteus spp. bacteria in natural environments. | proteus spp. bacteria were first described in 1885 by gustav hauser, who had revealed their feature of intensive swarming growth. currently, the genus is divided into proteus mirabilis, proteus vulgaris, proteus penneri, proteus hauseri, and three unnamed genomospecies 4, 5, and 6 and consists of 80 o-antigenic serogroups. the bacteria are known to be human opportunistic pathogens, isolated from urine, wounds, and other clinical sources. it is postulated that intestines are a reservoir of these ... | 2016 | 26748500 |
| structural basis for latency and function of immune inhibitor a metallopeptidase, a modulator of the bacillus anthracis secretome. | immune inhibitor a(inha)-type metallopeptidases are potential virulence factors secreted by members of the bacillus cereus group. two paralogs from anthrax-causing bacillus anthracis (bainha1 and bainha2) were shown to degrade host tissue proteins with broad substrate specificity. analysis of their activation mechanism and the crystal structure of a zymogenic bainha2 variant revealed a ∼750-residue four-domain structure featuring a pro-peptide, a catalytic domain, a domain reminiscent of viral e ... | 2016 | 26745529 |
| primer evaluation for pcr and its application for detection of carbapenemases in enterobacteriaceae. | during the last decade, the prevalence of carbapenem-resistant enterobacteriaceae in human patients has increased. carbapenemase-producing bacteria are usually multidrug resistant. therefore, early recognition of carbapenemase producers is critical to prevent their spread. | 2016 | 27099689 |
| laboratory detection and clinical implication of oxacillinase-48 like carbapenemase: the hidden threat. | carbapenemase producing gram-negative pathogen is of great concern for physician. the challenging aspects are treatment option and infection control. monitoring of respective carbapenemase resistance mechanism is necessary to prevent the outbreaks. currently, the rapid emergence of oxacillinase (oxa-48) like is alarming. increasing frequency of oxa-48 is seen than the classical carbapenemase (kpc, ndm, imp, and vim) across the world. the bla oxa-48 gene is commonly identified in escherichia coli ... | 2016 | 27013843 |
| enhancing phenotypic detection of esbl in ampc co-producers by using cefepime and tazobactam. | routine phenotypic methods employing clavulanate and third generation cephalosporins to detect esbl are not promising for isolates that co-produce an inhibitor-resistant beta lactamase like ampc. | 2016 | 26894064 |
| new hydrazines with sulphonamidic structure: synthesis,characterization and biological activity. | infections caused by bacterial species are common in immunocompromised patients and carry significant treatment costs and mortality. the emerging resistance of microorganisms to some synthetic antimicrobial agents makes it necessary to continue the research for new antimicrobial drugs. | 2016 | 24505922 |
| rapid molecular approach for simultaneous detection of salmonella spp., shigella spp., and vibrio cholera. | gastrointestinal tract infection is still one of the serious public health problems in many geographic areas and is endemic in most countries including iran. early detection of the gastrointestinal tract pathogens can be extremely important. the aim of the current study was to apply a shortened time-multiplex polymerase chain reaction (pcr) for rapid and simultaneous detection of salmonella spp., shigella spp., and vibrio cholera. | 2016 | 28053842 |
| beneficial effects of spices in food preservation and safety. | spices have been used since ancient times. although they have been employed mainly as flavoring and coloring agents, their role in food safety and preservation have also been studied in vitro and in vivo. spices have exhibited numerous health benefits in preventing and treating a wide variety of diseases such as cancer, aging, metabolic, neurological, cardiovascular, and inflammatory diseases. the present review aims to provide a comprehensive summary of the most relevant and recent findings on ... | 2016 | 27708620 |
| development of a fast and low-cost qpcr assay for diagnosis of acute gas pharyngitis. | group a streptococci (gas) are the most common bacterial cause of acute pharyngitis and account for 15-30 % of cases of acute pharyngitis in children and 5-10 % of cases in adults. in this study, a real-time quantitative pcr (qpcr) based gas detection assay in pharyngeal swab specimens was developed. | 2016 | 27501688 |
| the t6sss of pseudomonas aeruginosa strain pao1 and their effectors: beyond bacterial-cell targeting. | pseudomonas aeruginosa is an opportunistic pathogen responsible for many diseases such as chronic lung colonization in cystic fibrosis patients and acute infections in hospitals. the capacity of p. aeruginosa to be pathogenic toward several hosts is notably due to different secretion systems. amongst them, p. aeruginosa encodes three type six secretion systems (t6ss), named h1- to h3-t6ss, that act against either prokaryotes and/or eukaryotic cells. they are independent from each other and injec ... | 2016 | 27376031 |
| unique lipid anchor attaches vi antigen capsule to the surface of salmonella enterica serovar typhi. | polysaccharide capsules are surface structures that are critical for the virulence of many gram-negative pathogenic bacteria. salmonella enterica serovar typhi is the etiological agent of typhoid fever. it produces a capsular polysaccharide known as "vi antigen," which is composed of nonstoichiometrically o-acetylated α-1,4-linked n-acetylgalactosaminuronic acid residues. this glycan is a component of currently available vaccines. the genetic locus for vi antigen production is also present in so ... | 2016 | 27226298 |
| isolation of an aptamer that binds specifically to e. coli. | escherichia coli is a bacterial species found ubiquitously in the intestinal flora of animals, although pathogenic variants cause major public health problems. aptamers are short oligonucleotides that bind to targets with high affinity and specificity, and have great potential for use in diagnostics and therapy. we used cell-based systematic evolution of ligands by exponential enrichment (cell-selex) to isolate four single stranded dna (ssdna) aptamers that bind strongly to e. coli cells (atcc g ... | 2016 | 27104834 |
| fluoroquinolone-resistant enteric bacteria in sub-saharan africa: clones, implications and research needs. | fluoroquinolones came into widespread use in african countries in the early 2000s, after patents for the first generation of these drugs expired. by that time, quinolone antibacterial agents had been used intensively worldwide and resistant lineages of many bacterial species had evolved. we sought to understand which gram negative enteric pandemic lineages have been reported from africa, as well as the nature and transmission of any indigenous resistant clones. a systematic review of articles in ... | 2016 | 27148238 |
| automated broad-range molecular detection of bacteria in clinical samples. | molecular detection methods, such as quantitative pcr (qpcr), have found their way into clinical microbiology laboratories for the detection of an array of pathogens. most routinely used methods, however, are directed at specific species. thus, anything that is not explicitly searched for will be missed. this greatly limits the flexibility and universal application of these techniques. we investigated the application of a rapid universal bacterial molecular identification method, is-pro, to rout ... | 2016 | 26763956 |
| escherichia coli o104 in feedlot cattle feces: prevalence, isolation and characterization. | escherichia coli o104:h4, an hybrid pathotype of shiga toxigenic and enteroaggregative e. coli, involved in a major foodborne outbreak in germany in 2011, has not been detected in cattle feces. serogroup o104 with h type other than h4 has been reported to cause human illnesses, but their prevalence and characteristics in cattle have not been reported. our objectives were to determine the prevalence of e. coli o104 in feces of feedlot cattle, by culture and pcr detection methods, and characterize ... | 2016 | 27010226 |
| optimized use of the maldi biotyper system and the filmarray bcid panel for direct identification of microbial pathogens from positive blood cultures. | despite the current reliance on blood cultures (bcs), the diagnosis of bloodstream infections (bsis) can be sped up using new technologies performed directly on positive bc bottles. two methods (the maldi biotyper system and filmarray blood culture identification [bcid] panel) are potentially applicable. in this study, we performed a large-scale clinical evaluation (1,585 microorganisms from 1,394 bsi episodes) on the combined use of the maldi biotyper and filmarray bcid panel compared to a refe ... | 2016 | 26677254 |
| biodegradation of mycotoxins: tales from known and unexplored worlds. | exposure to mycotoxins, secondary metabolites produced by fungi, may infer serious risks for animal and human health and lead to economic losses. several approaches to reduce these mycotoxins have been investigated such as chemical removal, physical binding, or microbial degradation. this review focuses on the microbial degradation or transformation of mycotoxins, with specific attention to the actual detoxification mechanisms of the mother compound. furthermore, based on the similarities in che ... | 2016 | 27199907 |
| novel conopeptides of largely unexplored indo pacific conus sp. | cone snails are predatory creatures using venom as a weapon for prey capture and defense. since this venom is neurotoxic, the venom gland is considered as an enormous collection of pharmacologically interesting compounds having a broad spectrum of targets. as such, cone snail peptides represent an interesting treasure for drug development. here, we report five novel peptides isolated from the venom of conus longurionis, conus asiaticus and conus australis. lo6/7a and lo6/7b were retrieved from c ... | 2016 | 27801785 |
| methylome analysis of two xanthomonas spp. using single-molecule real-time sequencing. | single-molecule real-time (smrt) sequencing allows identification of methylated dna bases and methylation patterns/motifs at the genome level. using smrt sequencing, diverse bacterial methylomes including those of helicobacter pylori, lactobacillus spp., and escherichia coli have been determined, and previously unreported dna methylation motifs have been identified. however, the methylomes of xanthomonas species, which belong to the most important plant pathogenic bacterial genus, have not been ... | 2016 | 27904456 |
| extraction of total nucleic acids from bacterial isolates using the biomérieux nuclisens easymag total nucleic acid extractor. | the biomerieux nuclisens easymag total nucleic acid extractor was evaluated for use on bacterial isolates in the clinical microbiology laboratory. forty eight isolates were extracted, yielding quantifiable amounts of dna for all isolates. the easymag is appropriate for dna extraction from bacterial isolates and will be incorporated in the clinical laboratory. | 2016 | 27658494 |
| a retrospective study of pyogenic liver abscess focusing on klebsiella pneumoniae as a primary pathogen in china from 1994 to 2015. | pyogenic liver abscess (pla) is a common intra-abdominal infection in adults. in this study, we aim to explore demographic and clinical characteristics of pla focusing on klebsiella pneumoniae (k. pneumoniae) induced pla (kp-pla) in mainland china. a retrospective review of medical records from all patients with kp-pla admitted to a tertiary teaching hospital over a 21-year period (1994-2015) was performed. among 296 pla cases with confirmed culture-positive data, k. pneumoniae was revealed as t ... | 2016 | 27929082 |
| the iridica bac bsi assay: rapid, sensitive and culture-independent identification of bacteria and candida in blood. | bloodstream infection (bsi) and sepsis are rising in incidence throughout the developed world. the spread of multi-drug resistant organisms presents increasing challenges to treatment. surviving bsi is dependent on rapid and accurate identification of causal organisms, and timely application of appropriate antibiotics. current culture-based methods used to detect and identify agents of bsi are often too slow to impact early therapy and may fail to detect relevant organisms in many positive cases ... | 2016 | 27384540 |
| rapid identification and multiple susceptibility testing of pathogens from positive-culture sterile body fluids by a combined maldi-tof mass spectrometry and vitek susceptibility system. | infections of the bloodstream, central nervous system, peritoneum, joints, and other sterile areas are associated with high morbidity and sequelae risk. timely initiation of effective antimicrobial therapy is crucial to improving patient prognosis. however, standard final identification and antimicrobial susceptibility tests (asts) are reported 16-48 h after a positive alert. for a rapid, effective and low-cost diagnosis, we combined matrix-assisted laser desorption/ionization time of flight mas ... | 2016 | 27148212 |
| protein domain architectures provide a fast, efficient and scalable alternative to sequence-based methods for comparative functional genomics. | a functional comparative genome analysis is essential to understand the mechanisms underlying bacterial evolution and adaptation. detection of functional orthologs using standard global sequence similarity methods faces several problems; the need for defining arbitrary acceptance thresholds for similarity and alignment length, lateral gene acquisition and the high computational cost for finding bi-directional best matches at a large scale. we investigated the use of protein domain architectures ... | 2016 | 27703668 |
| review: diversity of microorganisms in global fermented foods and beverages. | culturalable and non-culturable microorganisms naturally ferment majority of global fermented foods and beverages. traditional food fermentation represents an extremely valuable cultural heritage in most regions, and harbors a huge genetic potential of valuable but hitherto undiscovered strains. holistic approaches for identification and complete profiling of both culturalable and non-culturable microorganisms in global fermented foods are of interest to food microbiologists. the application of ... | 2016 | 27047484 |
| fecal diagnostics in combination with serology: best test to establish stec-hus. | in the majority of pediatric patients, the hemolytic-uremic syndrome (hus) is caused by an infection with shiga toxin-producing escherichia coli (stec), mostly serotype o157. it is important to discriminate between hus caused by stec and complement-mediated hus (atypical hus) due to differences in treatment and outcome. as stec and its toxins can only be detected in the patient's stool for a short period of time after disease onset, the infectious agent may go undetected using only fecal diagnos ... | 2016 | 27240858 |
| isolation, antimicrobial susceptibility profile and detection of sul1, blatem, and blashv in amoxicillin-clavulanate-resistant bacteria isolated from retail sausages in kampar, malaysia. | due to the overuse of antibiotics in livestock as a growth-promoting agent, the emergence of multi-antibiotic resistant bacteria is becoming a concern. | 2016 | 27942365 |
| new β-lactamase inhibitors in the clinic. | given the serious medical burden of β-lactamases, many approaches are being used identify candidate agents for β-lactamase inhibition. here, we review two β-lactam-β-lactamase inhibitor (bl-bli) combinations, ceftolozane-tazobactam and ceftazidime-avibactam that recently entered the clinic. in addition, we focus on bl-bli combinations in preclinical development that have demonstrated activity in clinical isolates via susceptibility testing and/or in in vivo models of infection. we highlight only ... | 2016 | 27208767 |
| rapid antimicrobial susceptibility test for identification of new therapeutics and drug combinations against multidrug-resistant bacteria. | current antimicrobial susceptibility testing has limited screening capability for identifying empirical antibiotic combinations to treat severe bacterial infections with multidrug-resistant (mdr) organisms. we developed a new antimicrobial susceptibility assay using automated ultra-high-throughput screen technology in combination with a simple bacterial growth assay. a rapid screening of 5170 approved drugs and other compounds identified 25 compounds with activities against mdr klebsiella pneumo ... | 2016 | 27826141 |
| opportunistic infections in hiv-infected patients differ strongly in frequencies and spectra between patients with low cd4+ cell counts examined postmortem and compensated patients examined antemortem irrespective of the haart era. | aids-related mortality has changed dramatically with the onset of highly active antiretroviral therapy (haart), which has even allowed compensated hiv-infected patients to withdraw from secondary therapy directed against opportunistic pathogens. however, in recently autopsied hiv-infected patients, we observed that associations with a broad spectrum of pathogens remain, although detailed analyses are lacking. therefore, we focused on the possible frequency and spectrum shifts in pathogens associ ... | 2016 | 27611681 |
| dcia is an ancestral replicative helicase operator essential for bacterial replication initiation. | delivery of the replicative helicase onto dna is an essential step in the initiation of replication. in bacteria, dnac (in escherichia coli) and dnai (in bacillus subtilis) are representative of the two known mechanisms that assist the replicative helicase at this stage. here, we establish that these two strategies cannot be regarded as prototypical of the bacterial domain since dnac and dnai (dna[ci]) are present in only a few bacterial phyla. we show that dna[ci] was domesticated at least seve ... | 2016 | 27830752 |
| occurrence of plasmid- and chromosome-carried mcr-1 in waterborne enterobacteriaceae in china. | the aim of this study was to investigate the prevalence of the polymyxin resistance gene mcr-1 in enterobacteriaceae from environmental water sources in hangzhou, china. colistin-resistant bacteria were isolated from environmental water samples using an enrichment broth culture method, were screened for mcr-1, and then were analyzed for the location and transferability of mcr-1 isolates positive for mcr-1 were further examined to determine their susceptibility profiles and were screened for the ... | 2017 | 28559252 |
| characterization of oxa-48-like-producing enterobacteriaceae isolated from river water in algeria. | the spread of carbapenemase-producing enterobacteriaceae (cpe) is a significant problem for healthcare worldwide. the prevalence of carbapenem-resistant enterobacteriaceae (cpe) in water environments in algeria are unknown. the aim of this study was to screen for the presence of cpe isolates in the soummam river in bejaia, algeria. isolates of enterobacteriaceae recovered from twelve samples of river water and showing reduced susceptibility to carbapenems were included in this study. the isolate ... | 2017 | 28486169 |
| citrobacter portucalensis sp. nov., isolated from an aquatic sample. | a gram-stain-negative strain, a60t, isolated from a water well sample in portugal, was characterized phenotypically, genotypically and phylogenetically. phylogenetic analysis based on 16s rrna gene sequences indicated that strain a60t belonged to the genus citrobacter, and recn gene phylogeny revealed one strongly supported clade encompassing strain a60t and 13 other strains from public databases, distinct from currently recognized species of the genus citrobacter. furthermore, multilocus sequen ... | 2017 | 28857032 |
| construction of recombinant pdu metabolosome shells for small molecule production in corynebacterium glutamicum. | bacterial microcompartments have significant potential in the area of industrial biotechnology for the production of small molecules, especially involving metabolic pathways with toxic or volatile intermediates. corynebacterium glutamicum is an established industrial workhorse for the production of amino acids and has been investigated for the production of a variety of further value-added products. herein, we describe components for the establishment of bacterial microcompartments as production ... | 2017 | 28826205 |
| complete genome sequence of citrobacter freundii 705sk3, an oxa-48-encoding wastewater isolate. | we present the genome sequence of citrobacter freundii 705sk3, a wastewater isolate harboring an incl oxa-48-encoding plasmid. assembly of the genome resulted in a 5,242,839-bp circular chromosome (gc content, 52%) and two closed plasmids of 296,175 bp and 63, 458 bp in size. | 2017 | 28818914 |
| high prevalence of extended-spectrum β-lactamase-producing enterobacteriaceae in wild fish from the mediterranean sea in algeria. | we investigated the prevalence of extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae among wild fish from the coast of bejaia (algeria) in the mediterranean sea. | 2017 | 28805537 |
| antimicrobial resistance and cytotoxicity of citrobacter spp. in maanshan anhui province, china. | objectives:citrobacter spp. especially citrobacter freundii, is frequently causing nosocomial infections, and increasingly becoming multi-drug resistant (mdr). in this study, we aimed to determine the genetic diversity and relationships of citrobacter spp. from diarrheal patients and food sources, their antimicrobial resistance profiles and in vitro virulence properties. methods: sixty two citrobacter isolates, including 13 c. freundii, 41 c. youngae and eight c. braakii isolates, were obtained ... | 2017 | 28775715 |
| complete genome sequence of citrobacter freundii myophage mijalis. | citrobacter freundii is responsible for various opportunistic nosocomial infections. phage therapies against c. freundii may prove useful in human medicine for treatment of infections caused by the ubiquitous bacteria. here, we announce the complete genome sequence of the c. freundii felix o1-like myophage mijalis and present its features. | 2017 | 28774966 |
| ecthyma gangrenosum caused by citrobacter freundii. | a 55-year-old man undergoing chemotherapy for recurrent multiple myeloma presented with a 2-day history of bilateral lower leg rash with pain and oedema. on examination, there were numerous non-palpable retiform pruritic patches over both lower legs. skin pnch biopsy demonstrated a diffuse interstitial neutrophilic infiltrate with necrosis. peripheral blood and skin tissue cultures both isolated citrobacterfreundii, consistent with a rare form of ecthyma gangrenosum. the patient responded with a ... | 2017 | 28751433 |
| citrobacter freundii bacteremia: risk factors of mortality and prevalence of resistance genes. | multidrug-resistant strains of citrobacter have emerged, which carry amp-c β-lactamase (amp-c), broad-spectrum β-lactamase, extended-spectrum β-lactamase (esbl), and other resistance mechanisms. these strains are associated with a higher rate of in-hospital mortality. the object of this study is to determine the mortality risk factors, susceptibility pattern to antibiotics, and prevalence of resistance genes in patients with citrobacter freundii bacteremia. | 2017 | 28711438 |
| characterization of mobile genetic elements carrying vim-1 and kpc-2 carbapenemases in citrobacter freundii isolates in madrid. | carbapenemase producing citrobacter freundii (cpcf) infections are still uncommon in european countries. here we report a molecular study conducted in a tertiary care facility in southern madrid, spain, from 2009 to 2014 to investigate the epidemiology of cpcf. the blaimp-1,blaimp-2,blakpc,blandm,blaoxa-48,blavim-1 and blavim-2 genes were screened by pcr. molecular typing was carried out by pulsed-field gel electrophoresis analysis (pfge) and multilocus sequence typing (mlst). whole genome seque ... | 2017 | 28711315 |
| insights into a novel blakpc-2-encoding incp-6 plasmid reveal carbapenem-resistance circulation in several enterobacteriaceae species from wastewater and a hospital source in spain. | untreated wastewater, particularly from hospitals and other healthcare facilities, is considered to be a reservoir for multidrug-resistant bacteria. however, its role in the spread of antibiotic resistances in the human population remains poorly investigated. we used whole genome sequencing to analyze 25 kpc-2-producing enterobacteriaceae isolates from sewage water collected during a 3-year period and three clinical citrobacter freundii isolates from a tertiary hospital in the same collection ar ... | 2017 | 28702005 |
| honeybee (apis mellifera)-associated bacterial community affected by american foulbrood: detection of paenibacillus larvae via microbiome analysis. | honeybee (apis mellifera l.) workers act as passive vectors of paenibacillus larvae spores, which cause the quarantine disease american foulbrood (afb). we assessed the relative proportions of p. larvae within the honeybee microbiome using metabarcoding analysis of the 16 s rrna gene. the microbiome was analyzed in workers outside of the afb zone (control - afb0), in workers from asymptomatic colonies in an afb apiary (afb1), and in workers from colonies exhibiting clinical afb symptoms (afb2). ... | 2017 | 28698604 |
| characterization of the complete nucleotide sequences of inca/c2 plasmids carrying in809-like integrons from enterobacteriaceae isolates of wildlife origin. | a total of 18 enterobacteriaceae (17 from gulls and 1 from a clinical sample) collected from australia, carrying inca/c plasmids with the imp-encoding in809-like integrons, were studied. seven plasmids, being representatives of different origins, plasmid sizes, replicon combinations, and resistance genes, were completely sequenced. plasmid pec158, identified in a clinical escherichia coli st752 isolate, showed extensive similarity to type 2 inca/c2 plasmids. pec158 carried none of the blacmy-2-l ... | 2017 | 28696228 |
| gene cloning, characterization, and cytotoxic activity of methionine γ-lyase from clostridium novyi. | the exploitation of methionine-depleting enzyme methionine γ-lyase (mgl) is a promising strategy against specific cancer cells that are strongly dependent on methionine. to identify mgl from different sources with high catalytic activity and efficient anticancer action, we have expressed and characterized mgl from clostridium novyi and compared its catalytic efficiency with the previously studied mgl from citrobacter freundii. the purified recombinant mgl exhibits kcat and kcat /km for methionin ... | 2017 | 28681503 |
| wastewater is a reservoir for clinically relevant carbapenemase- and 16s rrna methylase-producing enterobacteriaceae. | the aim of this study was to evaluate wastewater for carbapenemase-producing enterobacteriaceae (cpe) and 16s rrna methylase-producing gram-negative bacteria (mpb) and to assess their occurrence following wastewater treatment. wastewater samples were collected between june 2015 and march 2016 in the sewage network of the city of basel (switzerland) from sites located before and after influx of wastewater from the hospital into the sewage network. samples were also obtained from the influent and ... | 2017 | 28668692 |
| prophylactic use of intraoperative vancomycin powder and postoperative infection: an analysis of microbiological patterns in 1200 consecutive surgical cases. | objective wound infections following spinal surgery for deformity place a high toll on patients, providers, and the health care system. the prophylactic application of intraoperative vancomycin powder has been shown to lower the infection risk after thoracolumbar decompression and fusion for deformity correction. the purpose of this study was to assess the microbiological patterns of postoperative surgical site infections (ssis) after prophylactic use of vancomycin powder in adult patients under ... | 2017 | 28665245 |
| extended-spectrum β-lactamases and plasmid-mediated quinolone resistance in enterobacterial clinical isolates from neonates in tunisia. | this study was conducted to investigate extended-spectrum-β-lactamase (esbl) producing enterobacteriaceae isolates from the center of maternity and neonatology of monastir, tunisia. fourty-six strains out of 283 were found to produce esbl: klebsiella pneumoniae (n = 37), escherichia coli (n = 6), enterobacter cloacae (n = 2), and citrobacter freundi (n = 1). genotyping analysis, using eric2 and rapd, showed that strains were clonally unrelated. pcr amplification followed by sequencing revealed t ... | 2017 | 28648624 |
| molecular and microbiological report of a hospital outbreak of ndm-1-carrying enterobacteriaceae in mexico. | to characterize the microbiological, molecular and epidemiological data of an outbreak of carbapenem-resistant enterobacteriaceae (cre) in a tertiary-care hospital in mexico. | 2017 | 28636666 |
| genomic and phenotypic characterisation of fluoroquinolone resistance mechanisms in enterobacteriaceae in durban, south africa. | resistance to fluoroquinolones (fq) is being increasingly reported and found to be mediated by efflux pumps, plasmid-mediated quinolone resistance genes (pmqr) and mutations in gyra, gyrb, parc and pare. however, studies reporting on fq resistance mechanisms (fqrm), particularly in africa, are focused mostly on salmonella. this study used a whole-genome-based approach to describe fqrm in forty-eight clinical enterobacteriaceae isolates comprising of klebsiella pneumoniae (n = 21), serratia marce ... | 2017 | 28636609 |
| citrobacter freundii as a cause of acute suppurative thyroiditis in an immunocompetent adult female. | acute suppurative thyroiditis (ast) is an uncommon condition, in the patients with preexisting thyroid disease or immunosuppression. the most common cause of ast is bacterial, and the most common bacteria are staphylococcus aureus, staphylococcus epidermidis, escherichia coli, and klebsiella species. due to various complications such as septicemia, septic thrombophlebitis, necrotizing mediastinitis, or pericarditis, prompt diagnosis and treatment are the keys to reduce mortality and morbidity. h ... | 2017 | 28631657 |
| the bactericidal effect of simultaneous titanium oxide on common hospital bacteria. | bacteria prevalence has increased in health centers and hospitals. infection control can decrease the number of bacteria. bacteria cause 90% of nosocomial infections and fungi, viruses, or protozoa are less involved. bacteria control can decrease the number of diseases. the simultaneous titanium oxide (smtio) is a new method of disinfection. this process degrades bacteria by producing hydroxyl radical. the present study was conducted with the aim of analyzing the bactericidal effect of simultane ... | 2017 | 28623574 |
| crystal structure of mutant form cys115his of citrobacter freundii methionine γ-lyase complexed with l-norleucine. | the mutant form of citrobacter freundii methionine γ-lyase with the replacement of active site cys115 for his has been found to be inactive in the γ-elimination reaction of methionine while fully active in the γ-elimination reaction of o-acetyl-l-homoserine and in the β-elimination reaction of s-alk(en)yl-substituted cysteines. in this work, the crystal structure of the mutant enzyme complexed with competitive inhibitor, l-norleucine was determined at 1.45å resolution. at the enzyme active site ... | 2017 | 28602917 |
| emergence of a multidrug-resistant citrobacter freundii st8 harboring an unusual vim-4 gene cassette in poland. | the growing incidence of multidrug-resistant (mdr) bacteria is an emerging challenge in modern medicine. the utility of carbapenems, which are considered 'last-line' agents, is being diminished by the growing incidence of various resistance mechanisms in gram-negative bacteria. a molecular investigation was performed of an mdr carbapenem-resistant citrobacter freundii of sequence type 8 (st8) isolated from a hematology patient with acute myeloid leukemia. | 2017 | 28602727 |
| circulation of blakpc-3-carrying incx3 plasmids among citrobacter freundii isolates in an italian hospital. | colonizations due to carbapenem-resistant enterobacteriaceae (cre) are a source of antimicrobial resistance transmission in health care settings. eleven citrobacter freundii strains producing kpc-3 carbapenemase were isolated from rectal swabs during a 3-year surveillance program. blakpc-3-carrying plasmids were found to belong to the incx3 group in 9 of the 11 strains, and complete nucleotide sequences were obtained for 2 of them. our results highlight the possible role of c. freundii as reserv ... | 2017 | 28559268 |
| dissemination of bla oxa-370 gene among several enterobacteriaceae species in brazil. | oxa-370 is a recently described oxa-48 variant that has only been described in a few enterobacter spp. and klebsiella pneumoniae isolates. the purpose of this study is to assess the prevalence of oxa-370-producing isolates in carbapenem-nonsusceptible enterobacteriaceae recovered from 28 hospitals from brazil. real-time pcr was used to determine the presence of bla ndm-1, bla kpc-2, bla vim-type, bla ges-type, bla oxa-48-like, and bla imp-type genes. a total of 4,451 enterobacteriaceae were scre ... | 2017 | 28555403 |
| emergence of the colistin resistance gene mcr-1 in citrobacter freundii. | 2017 | 28433744 | |
| sequencing of blaimp-carrying incn2 plasmids, and comparative genomics of incn2 plasmids harboring class 1 integrons. | this work presents the complete nucleotide sequences of p0801-imp from klebsiella pneumoniae, p7121-imp from k. oxytoca, and p17285-imp from citrobacter freundii, which are recovered from three different cases of nosocomial infection. these three plasmids represent the first fully sequenced blaimp-carrying incn2 plasmids. further comparative genomics analysis of all the five integron-carrying incn2 plasmids p0801-imp, p7121-imp, p17285-imp, pjie137, and p34983-59.134kb indicates that they posses ... | 2017 | 28424761 |
| multiresistant opportunistic pathogenic bacteria isolated from polluted rivers and first detection of nontuberculous mycobacteria in the algerian aquatic environment. | opportunistic infections constitute a major challenge for modern medicine mainly because the involved bacteria are usually multiresistant to antibiotics. most of these bacteria possess remarkable ability to adapt to various ecosystems, including those exposed to anthropogenic activities. this study isolated and identified 21 multiresistant opportunistic bacteria from two polluted rivers, located in algiers. cadmium, lead, and copper concentrations were determined for both water samples to evalua ... | 2017 | 28771154 |
| arsenic-resistant and plant growth promoting firmicutes and γ-proteobacteria species from industrially polluted irrigation water and corresponding cropland. | the aim of the study was to explore irrigation water polluted with industrial waste and corresponding cropland to screen bacteria for as-detoxification and plant growth promotion. | 2017 | 28708308 |
| an unusual culprit: klebsiella pneumoniae causing septicaemia outbreaks in neonatal pigs? | this study investigated the cause of recent outbreaks of septicaemia in neonatal pigs in australia (victoria and queensland). septicaemia in neonatal pigs is commonly caused by enterotoxigenic e. coli, extraintestinal pathogenic e. coli and beta-haemolytic streptococci. infrequent causes of septicaemia are actinobacillus suis and citrobacter freundii. therefore, it was quite unexpected when klebsiella pneumoniae was isolated in predominant growth from multiple organs of pigs dying of septicaemia ... | 2017 | 28619154 |
| in vitro selection and identification of potential probiotics isolated from the gastrointestinal tract of nile tilapia, oreochromis niloticus. | fish gut bacteria can be used as probiotics for aquaculture. the aim of this study is to screen and identify beneficial probiotic bacteria from the gut of nile tilapia, oreochromis niloticus. nine out of one hundred thirty-five isolates were non-pathogenic through intraperitoneal injection and had antibacterial activities with at least a strain from the five isolated fish pathogens, aeromonas sobria, aeromonas hydrophila, pseudomonas aeruginosa, pseudomonas putida, and staphylococcus aureus. fur ... | 2017 | 28819794 |
| antimicrobial property of lemongrass (cymbopogon citratus) oil against pathogenic bacteria isolated from pet turtles. | the usage of essential oils as antimicrobial agents is gaining attention. besides, pet turtles were known to harbor a range of pathogenic bacteria while the turtle keeping is a growing trend worldwide.the current study examined the antimicrobial activity of lemon grass oil (lgo) against seven species of gram negative bacteria namely; aeromonas hydrophila, a. caviae, citrobacter freundii, salmonella enterica, edwardsiella tarda, pseudomonas aeruginosa, and proteus mirabilis isolated from three po ... | 2017 | 28747972 |
| antimicrobial activity of endophytic bacterial populations isolated from medical plants of iran. | endophytic actinobacteria colonize inside the plant tissues without causing damages to the host plant. since these microorganisms colonize in the different parts of plants and can stop plant disease, they have been applied as biological agents for controlling human diseases. the aim of this study was molecular identification and measuring the antimicrobial activity of endophytic actinomycetes isolated from medicinal plants of iran. | 2017 | 28775818 |
| saccharopolyspora hattusasensis sp. nov., isolated from soil. | a saccharopolyspora strain, designated cr3506(t), isolated from a soil sample collected from sungurlu, corum, turkey, was examinated using a polyphasic approach. phylogenetic analysis based on an almost-complete 16s rrna gene sequence analysis showed that the strain is closely related to the type strains of saccharopolyspora spinosa nrrl 18395(t) (99.1%), saccharopolyspora phatthalungensis nrrl b-24798(t) (98.4%) and saccharopolyspora shandongensis 88(t) (98.1%); low levels of dna-dna relatednes ... | 2017 | 28785826 |
| occurrence of extended-spectrum and ampc β-lactamases in multiple drug resistant salmonella isolates from clinical samples in lagos, nigeria. | salmonella spp. are important foodborne pathogens exhibiting increasing resistance to antimicrobial drugs. resistance to broad-spectrum β-lactams, mediated by extended-spectrum β-lactamase (esbl) and ampc β-lactamase enzymes is fast spreading and has had negative impacts on the clinical outcomes, particularly on third-generation cephalosporins. this study investigated the carriage of ampc gene among multidrug-resistant salmonella spp. from lagos, nigeria. | 2017 | 28144154 |
| osteopathic treatment in a patient with left-ventricular assist device with left brachialgia: a case report. | this study deals with an osteopathic approach used for a patient with left-ventricular assist device (l-vad) affected by left brachialgia. clinical examination revealed the presence of thoracic outlet syndrome and pectoralis minor syndrome, with compression of the left proximal ulnar nerve, related to the surgical sternotomy performed. the osteopathic techniques used can be classified as indirect and direct, addressed to the pectoralis minor and the first left rib, respectively. to our knowledge ... | 2017 | 28144166 |
| development of an algorithm for phenotypic screening of carbapenemase-producing enterobacteriaceae in the routine laboratory. | carbapenemase-producing enterobacteriaceae (cpe) are difficult to identify among carbapenem non-susceptible enterobacteriaceae (nse). we designed phenotypic strategies giving priority to high sensitivity for screening putative cpe before further testing. | 2017 | 28095794 |
| characterization of two polyvalent phages infecting enterobacteriaceae. | bacteriophages display remarkable genetic diversity and host specificity. in this study, we explore phages infecting bacterial strains of the enterobacteriaceae family because of their ability to infect related but distinct hosts. we isolated and characterized two novel virulent phages, sh6 and sh7, using a strain of shigella flexneri as host bacterium. morphological and genomic analyses revealed that phage sh6 belongs to the t1virus genus of the siphoviridae family. conversely, phage sh7 was cl ... | 2017 | 28091598 |
| implementation of short incubation maldi-tof ms identification from positive blood cultures in routine diagnostics and effects on empiric antimicrobial therapy. | results of blood culture (bc) diagnostics should be swiftly available to guide treatment of critically ill patients. conventional bc diagnostics usually performs species identification of microorganisms from mature solid medium colonies. species identification might be speed up by using matrix-assisted laser desorption ionization time-of-flight (maldi-tof) mass spectrometry (ms) of biomass from shortly incubated solid media. | 2017 | 28101334 |
| the influence of antibiotic prophylaxis on bacterial resistance in urinary tract infections in children with spina bifida. | bacterial resistance to antibiotics is an increasingly threatening consequence of antimicrobial exposure for many decades now. in urinary tract infections (utis), antibiotic prophylaxis (ap) increases bacterial resistance. we studied the resistance patterns of positive urinary cultures in spina bifida children on clean intermittent catheterization, both continuing and stopping ap. | 2017 | 28081719 |
| evaluation of the β-carba™ test, a colorimetric test for the rapid detection of carbapenemase activity in gram-negative bacilli. | there is an urgent need for accurate and fast diagnostic tests to identify carbapenemase-producing bacteria. here, we have evaluated a novel colorimetric test (the β-carba™ test; bio-rad) to detect carbapenemase-producing gram-negative bacilli from cultured colonies. | 2017 | 28333363 |
| antimicrobial activity, ame resistance, and a-site binding studies of anthraquinone-neomycin conjugates. | the antibacterial effects of aminoglycosides are based on their association with the a-site of bacterial rrna and interference with the translational process in the bacterial cell, causing cell death. the clinical use of aminoglycosides is complicated by resistance and side effects, some of which arise from their interactions with the human mitochondrial 12s rrna and its deafness-associated mutations, c1494u and a1555g. we report a rapid assay that allows screening of aminoglycoside compounds to ... | 2017 | 28103015 |
| prevalence of multidrug resistant uropathogenic bacteria in pediatric patients of a tertiary care hospital in eastern india. | today, because systemic infections such as urinary tract infection (uti) affect even pediatric patients, antibiotic resistant bacteria have become a constant clinical challenge. in the present study, a total of 1054 urine samples were collected from pediatric patients over 18 months. from these samples, 510 isolates of pathogenic bacteria were collected using hicrome uti agar. antibiotic sensitivity tests of isolates were performed using the kirby-bauer method. two gram-positive bacteria (entero ... | 2017 | 26617250 |
| serum bactericidal assay: new role in salmonella detection. | while inspecting animal feed for salmonella contamination, we routinely observed bacterial colonies on selective agars that were similar in appearance to those formed by salmonella. these were identified as citrobacter freundii, proteus mirabilis, and serratia fonticola using biochemical and serological techniques. because the presence of these bacterial species confounds identification of salmonella, we refer to them as "interference bacteria." polyvalent antisera against these interference bac ... | 2017 | 26822285 |
| risk of infection transmission in curvilinear array echoendoscopes: results of a prospective reprocessing and culture registry. | the complex design of the elevator mechanism in duodenoscopes has been recognized as a challenge for disinfection and recently implicated as a potential source of persistent bacterial contamination. curvilinear array (cla) echoendoscopes also have an elevator mechanism; however, there are no recommendations or data regarding the risk of persistent bacterial contamination of echoendoscopes. here we hoped to determine the yield of microbial growth with routine bacterial surveillance cultures of re ... | 2017 | 27478029 |
| engineered citrobacter freundii methionine γ-lyase effectively produces antimicrobial thiosulfinates. | antimicrobial activity of thiosulfinates in situ produced by mixtures of citrobacter freundii methionine γ-lyase (mgl) with new substrates, l-methionine and s-(alkyl/allyl)-l-cysteine sulfoxides has been recently demonstrated (anufrieva et al., 2015). this opens a way to the rational design of a new biotechnologically relevant antimicrobial drug producer. to increase the efficiency of the enzyme toward sulfoxides, the mutant forms of mgl, with the replacements of active site cysteine 115 with al ... | 2017 | 27430732 |
| citrobacter freundii as a test platform for recombinant cellulose degradation systems. | cellulosic biomass represents a huge reservoir of renewable carbon, but converting it into useful products is challenging. attempts to transfer cellulose degradation capability to industrially useful micro-organisms have met with limited success, possibly due to poorly understood synergy between multiple cellulases. this is best studied by co-expression of many combinations of cellulases and associated proteins. here, we describe the development of a test platform based on citrobacter freundii, ... | 2017 | 27617802 |
| incn st7 epidemic plasmid carrying blaimp-4 in enterobacteriaceae isolates with epidemiological links to multiple geographical areas in china. | to characterize blaimp-4-carrying plasmids originating from inpatients in hong kong. | 2017 | 27609049 |
| purification, characterization and functional analysis of the immune molecule lectin from the haemolymph of blue swimmer crab portunus pelagicus and their antibiofilm properties. | the present study reveals purification and characterization of immune molecule lectin from the haemolymph of blue swimmer crab portunus pelagicus (pp-lec). the pp-lec was purified by affinity chromatography with mannose coupled sepharose cl-4b column and it exhibits single band with a molecular weight of 155 kda in sds-page. the surface morphology of purified pp-lec displays the homogeneous nature of protein. a distinct peak with a retention time of 3.3 min was appeared in high performance liqui ... | 2017 | 28110033 |
| broadly reactive aptamers targeting bacteria belonging to different genera using a sequential toggle cell-selex. | conventional cell-selex aims to isolate aptamers to a single unique target bacteria species. we propose a method to isolate single-stranded dna aptamers that have broad reactivity to multiple bacterial targets belonging to different genera. the key of the proposed method is that targets of interest are changed sequentially at each selex round. the general scheme was examined using six bacteria from different genera, escherichia coli, enterobacter aerogenes, klebsiella pneumoniae, citrobacter fre ... | 2017 | 28272554 |
| sequencing and comparative genomics analysis of the inchi2 plasmids pt5282-mpha and p112298-cata and the inchi5 plasmid pynkp001-dfra. | incompatibility group inchi plasmids are important vectors of antibiotic resistance in enterobacteriaceae. in this study, a scheme for typing inchi into five separately clustering subgroups, including previously designated inchi1-3 as well as inchi4-5, was proposed based on sequenced inchi plasmids. the complete nucleotide sequences of the inchi2 plasmids pt5282-mpha and p112298-cata and the inchi5 plasmid pynkp001-dfra from clinical enterobacter cloacae, citrobacter freundii and raoultella orni ... | 2017 | 28390961 |
| comparative genomic analysis reveals organization, function and evolution of ars genes in pantoea spp. | numerous genes are involved in various strategies to resist toxic arsenic (as). however, the as resistance strategy in genus pantoea is poorly understood. in this study, a comparative genome analysis of 23 pantoea genomes was conducted. two vertical genetic arsc-like genes without any contribution to as resistance were found to exist in the 23 pantoea strains. besides the two arsc-like genes, as resistance gene clusters arsrbc or arsrbch were found in 15 pantoea genomes. these ars clusters were ... | 2017 | 28377759 |
| multiregional dissemination of kpc-producing klebsiella pneumoniae st258/st512 genotypes in poland, 2010-14. | in 2008-09, the kpc carbapenemase epidemiology in poland was dominated by a klebsiella pneumoniae st258 kpc-2 outbreak in warsaw and its administrative region. the aim of this study was to analyse the situation in 2010-14, with a focus on new outbreaks in other parts of the country. | 2017 | 28333331 |
| bacterial cellulose-zinc oxide nanocomposites as a novel dressing system for burn wounds. | bacterial cellulose possesses physical and mechanical properties of an ideal wound dressing material but lack of antimicrobial activity limits its biomedical applications. therefore, in current study, the inherent wound healing characteristics of bacterial cellulose and antimicrobial properties of zinc oxide nanoparticles were combined. the reinforcement (impregnation) of zinc oxide nanoparticles into bacterial cellulose sheets was confirmed through various characterization techniques. the antim ... | 2017 | 28325319 |
| draft genome sequence of citrobacter freundii strain a47, resistant to the mycotoxin deoxynivalenol. | here, we present the draft genome sequence of citrobacter freundii strain a47 with a length of 4,878,242 bp, which contains 4,357 putative protein coding genes, including 270 unique genes. this work is expected to assist in obtaining novel gene(s) that code for deoxynivalenol (don) de-epoxidation enzyme(s). | 2017 | 28302773 |
| determination of free polysaccharide in vi glycoconjugate vaccine against typhoid fever. | glycoconjugate vaccines based on the vi capsular polysaccharide directed against salmonella enterica serovar typhi are licensed or in development against typhoid fever, an important cause of morbidity and mortality in developing countries. quantification of free polysaccharide in conjugate vaccines is an important quality control for release, to monitor vaccine stability and to ensure appropriate immune response. however, we found that existing separation methods based on size are not appropriat ... | 2017 | 28282600 |
| impact of revised broad-spectrum cephalosporin clinical and laboratory standards institute breakpoints on susceptibility in enterobacteriaceae producing ampc β-lactamase. | we evaluated the impact of revised clinical and laboratory standards institute (clsi) breakpoints for broad-spectrum cephalosporins (bscs) on the susceptibilities of 1,742 isolates of enterobacter species, serratia marcescens, citrobacter freundii, and morganella morganii. the 2011 clsi criteria for cefotaxime and ceftazidime reduced the rates of susceptibility by 2.9% and 5.9%, respectively. the 2014 clsi criteria for cefepime reduced the rate of susceptibility by 13.9%, and categorized 11.8% i ... | 2017 | 28271652 |
| carbonyl cyanide m-chlorophenylhydrazine (cccp) reverses resistance to colistin, but not to carbapenems and tigecycline in multidrug-resistant enterobacteriaceae. | background: carbapenems (car), colistin (cst), and tigecycline (tgc) alone or in combination therapy has become the last-resort antibiotics for treating infections caused by multidrug resistant (mdr) bacteria. however, resistance to these reserve antibiotics are increasingly being reported worldwide. hence, the quest to find other agents that will synergistically restore the efficacy of these antibiotics have increased. methods: sixty-three clinical enterobacteriaceae isolates comprising of kleb ... | 2017 | 28261184 |
| the sink as a potential source of transmission of carbapenemase-producing enterobacteriaceae in the intensive care unit. | carbapenemase-producing enterobacteriaceae (cpe) are emerging pathogens that represent a major public health threat. in the university hospital of brussels, the incidence of new patients with cpe rose from 1 case in 2010 to 35 cases in 2015. between january and august 2015, five patients became infected/colonized with cpe during their stay in the same room in the intensive care unit (icu). since the time period between those patients was relatively short and the strains belonged to different spe ... | 2017 | 28239453 |
| soil type, soil moisture, and field slope influence the horizontal movement of salmonella enterica and citrobacter freundii from floodwater through soil. | pathogens in soil are readily mobilized by infiltrating water to travel downward through the soil. however, limited data are available on the horizontal movement of pathogens across a field. this study used a model system to evaluate the influence of soil type, initial soil moisture content, and field slope on the movement of salmonella enterica serovar newport across a horizontal plane of soil under flooding conditions. three soil types of varying clay content were moistened to 40, 60, or 80% o ... | 2017 | 28221887 |
| development of a high-throughput method to evaluate serum bactericidal activity using bacterial atp measurement as survival readout. | serum bactericidal activity (sba) assay is the method of choice to evaluate the complement-mediated functional activity of both infection- and vaccine-induced antibodies. to perform a typical sba assay, serial dilutions of sera are incubated with target bacterial strains and complement. the conventional sba assay is based on plating on agar the sba reaction mix and counting the surviving bacterial colony forming units (cfu) at each serum dilution. even with automated colony counting, it is labor ... | 2017 | 28192483 |
| plasma methionine depletion and pharmacokinetic properties in mice of methionine γ-lyase from citrobacter freundii, clostridium tetani and clostridium sporogenes. | pk studies were carried out after a single i.v. administration of 500 and 1000 u/kg by measuring of mgl activity in plasma samples. l-methionine concentration was measured by mass spectrometry. after single i.v. injection of 500u/kg the circulating t1/2 of enzymes in mice varies from 73 to 123min. the auc0-tinf values determined for mgl 500u/kg from c. freundii, c. tetani and c. sporogenes are 8.21±0.28, 9.04±0.33 and 13.88±0.39u/(ml×h), respectively. comparison of pk parameters of three mgl sou ... | 2017 | 28178629 |
| global molecular epidemiology of imp-producing enterobacteriaceae. | international data on the molecular epidemiology of enterobacteriaceae with imp carbapenemases are lacking. we performed short-read (illumina) whole-genome sequencing on a global collection of 38 imp-producing clinical enterobacteriaceae (2008 to 2014). imp-producing enterobacteriaceae (7 varieties within 11 class 1 integrons) were mainly present in the south pacific and asia. specific blaimp-containing integrons (in809 with blaimp-4, in722 with blaimp-6, and in687 with blaimp-14) were circulati ... | 2017 | 28167555 |