Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| unscheduled dna replication origin activation at inserted hpv 18 sequences in a hpv-18/myc amplicon. | oncogene amplification is a critical step leading to tumorigenesis, but the underlying mechanisms are still poorly understood. despite data suggesting that dna replication is a major source of genomic instability, little is known about replication origin usage and replication fork progression in rearranged regions. using a single dna molecule approach, we provide here the first study of replication kinetics on a previously characterized myc/papillomavirus (hpv18) amplicon in a cervical cancer. u ... | 2007 | 17444495 |
| case report of a kidney transplant recipient converted to everolimus due to malignancy: resolution of bronchiolitis obliterans organizing pneumonia without everolimus discontinuation. | the use of proliferative signal inhibitors (psis) in immunosuppression-related malignancies opens new roads for increasing the survival and quality of life in patients with solid organ transplantation. a 56-year-old female recipient of a living donor renal allograft (1990), who was immunosuppressed with cyclosporine (csa; neoral), azathioprine, and steroids, did initially well with acceptable renal function. during the last 5 years she required local therapy due to posterior vaginal lip human pa ... | 2007 | 17445552 |
| human papillomavirus in high- and low-risk areas of oesophageal squamous cell carcinoma in china. | to examine the potential roles of human papillomavirus (hpv) in oesophageal squamous cell carcinoma (escc) development, we examined the presence of hpv dna in paraffin-embedded escc tissues collected from two areas with different escc incidence rates in china, that is, gansu (n=26) and shandong (n=33), using pcr with spf10 primers, or pcr with gp5+/gp6+ primers combined with southern blot hybridisation. hpv genotype was determined by the inno-lipa hpv genotyping kit. hpv dna was detected in 17 c ... | 2007 | 17453003 |
| prospective evaluation of the hybrid capture 2 and amplicor human papillomavirus (hpv) tests for detection of 13 high-risk hpv genotypes in atypical squamous cells of uncertain significance. | the use of high-risk human papillomavirus (hrhpv) testing as an adjunct to cervical cytology in population-based screening programs is currently based on dna hybridization and pcr assays. the aim of this study was to prospectively assess the diagnostic performance of the hybrid capture 2 test (hc2; digene corporation) in comparison with that of the recently developed pcr-based amplicor hpv test (roche molecular systems) for the detection of 13 hrhpv types. a reverse line blot hybridization assay ... | 2007 | 17122007 |
| multiple cd4 and cd8 t-cell activation parameters predict vaccine efficacy in vivo mediated by individual dc-activating agonists. | a systematic comparison of the immunostimulatory capacity of tlr 2, 3, 4, 5, 7 and 9 agonists and an agonistic cd40-specific antibody was performed in a single long peptide vaccination model. all adjuvants activated dc in vitro but not all induced a strong functional t-cell response in vivo. optimal clonal cd8(+) t-cell expansion depended on the capacity of agonists to mature pro-inflammatory dc and the duration of their in vivo stimulatory effect. strong agonists promoted the induction of both ... | 2007 | 17123670 |
| human papillomavirus (hpv) types 101 and 103 isolated from cervicovaginal cells lack an e6 open reading frame (orf) and are related to gamma-papillomaviruses. | complete genomes of hpv101 and hpv103 were pcr amplified and cloned from cervicovaginal cells of a 34-year-old female with cervical intraepithelial neoplasia grade 3 (cin 3) and a 30-year-old female with a normal pap test, respectively. hpv101 and hpv103 contain 4 early genes (e7, e1, e2, and e4) and 2 late genes (l2 and l1), but both lack the canonical e6 orf. pairwise alignment similarity of the l1 orf nucleotide sequences of hpv101 and hpv103 indicated that they are at least 30% dissimilar to ... | 2007 | 17125811 |
| prevalence of human papillomavirus in skin tumors from repair deficient xeroderma pigmentosum patients. | the predisposition to skin cancers in childhood is the hallmark of xeroderma pigmentosum (xp), a rare autosomal recessive disorder, deficient in dna repair and hypersensitive to ultraviolet irradiation. human papillomavirus (hpvs), are common infections of the skin which are often found associated to benign lesions and non-melanoma skin cancers (nmsc), mainly squamous cell carcinomas (scc) and basal cell carcinomas (bcc). our study is the first to analyse 40 sccs, 27 bccs and nine normal skin bi ... | 2007 | 17126994 |
| hpv16 e6 confers p53-dependent and p53-independent phenotypes in the epidermis of mice deficient for e6ap. | high-risk human papillomaviruses are the causative agents of cervical and other anogenital cancers. in these cancers, two viral oncogenes, e6 and e7, are expressed. e6 is best known for its ability to inactivate the tumor suppressor p53, which is thought to arise through ubiquitin-mediated degradation of p53 and involve a ternary complex between e6, p53 and the e3 ligase, e6ap. in mice transgenic for wild-type hpv16 e6, its expression leads to epithelial hyperplasia and an abrogation of normal c ... | 2007 | 17130828 |
| human papillomaviruses in oral squamous cell carcinoma and pre-cancerous lesions detected by pcr-based gene-chip array. | human papillomavirus (hpv) infection is a significant risk factor for uterine cervical carcinoma. many studies have also demonstrated the presence of hpv in oral epithelia tissue, but the role of hpv infection in oral squamous cell carcinoma (oscc) is still controversial. the aim of this study was to determine the frequency and type of hpv in oscc and oral pre-cancerous lesions. dna samples were collected by cytobrushing from 51 patients with oscc, 46 with oral pre-cancerous lesions and 90 norma ... | 2007 | 17110084 |
| hypermethylation of two consecutive tumor suppressor genes, blu and rassf1a, located at 3p21.3 in cervical neoplasias. | although initiated by human papillomavirus (hpv), cervical carcinogenesis demands other cofactors to shape its natural course. epigenetic effects such as dna methylation, are considered to contribute to carcinogenesis process. | 2007 | 17097722 |
| the association between knowledge of hpv and feelings of stigma, shame and anxiety. | to test the hypotheses that (1) women who know that human papillomavirus (hpv) is sexually transmitted will expect to experience higher levels of stigma, shame and anxiety if they test positive for the virus than women who are not aware of the mode of transmission and (2) women who are aware of the high prevalence of hpv infection will expect to experience lower levels of stigma, shame and anxiety than women who underestimate its prevalence. | 2007 | 17098767 |
| modified hpv16 e7/hsp70 dna vaccine with high safety and enhanced cellular immunity represses murine lung metastatic tumors with downregulated expression of mhc class i molecules. | to explore whether the modified e7-hsp70, which has been introduced mutations in two zinc-binding motifs of e7, will eliminate its transformation potential and enhance the immunogenicity of fusion protein and repress e7 containing tumors with a low level of mhc-i molecules to lung metastatic in murine model. | 2007 | 17081598 |
| human papillomavirus is commonly present in psoriatic skin and normal skin samples from healthy subjects. | 2007 | 17081733 | |
| penile, urethral, and seminal sampling for diagnosis of human papillomavirus infection in men. | methods that used specimens from three genital sites (penile brushing [pb], urethral brushing [ub], and the retrieval of semen [se]) from 50 men were examined for human papillomavirus (hpv) dna detection. the rates of detection by pb, ub, se, pb and ub, and pb and se were 88.9%, 50.0%, 33.3%, 100%, and 97.2%, respectively. the use of pb and ub appears to be the most accurate method; as an alternative to ub, the use of se with pb could be used to improve the rate of hpv dna detection in men. | 2007 | 17093029 |
| risk of cervical cancer associated with chlamydia trachomatis antibodies by histology, hpv type and hpv cofactors. | human papillomavirus (hpv) is the central etiologic factor for cervical cancer, and prior studies suggested c. trachomatis may act as an hpv cofactor. we examined the c. trachomatis-cervical cancer association by serotype, histology, hpv type in the tumor, and other hpv cofactors. we conducted a population-based study in the seattle-puget sound area of 302 women with invasive squamous cell carcinomas (scc), 185 women with adenocarcinomas of the cervix (ac), and 318 hpv seropositive control women ... | 2007 | 17096345 |
| whole genome tiling path array cgh analysis of segmental copy number alterations in cervical cancer cell lines. | cervical cancer is the second most common malignancy in women worldwide, with high risk subtypes of human papillomavirus (hpv) constituting the major etiological agent. however, only a small percentage of women infected by the virus develop disease, suggesting that additional host genetic alterations are necessary for disease progression. in this study we examined the genomes of a panel of commonly used model cervical cancer cell lines using a recently developed whole genome tiling path array fo ... | 2007 | 17096350 |
| hpv-18 e7 conjugates to c-myc and mediates its transcriptional activity. | several reports in the literature have indicated that the e6 not only elevates the level of c-myc level but that the protein also associates with the myc complex and activates myc-responsive genes. there would seem to be a mechanism by which this oncogene can modulate cell proliferation and differentiation. furthermore, an increase in c-myc levels has also observed during ectopic expression of hpv e7 alone. using the yeast two-hybrid system, we further found that the c-myc interacts and forms a ... | 2007 | 17070091 |
| gadd45alpha does not modulate the carboplatin or 5-fluorouracil-induced apoptosis in human papillomavirus-positive cells. | gadd45alpha is shown to be induced by a wide spectrum of dna-damaging agents and implicated in negative regulation of cell growth by causing g2-m arrest or induction of apoptosis. in the present study, we explored the involvement of p53 in the promoter activation of gadd45alpha as well as the role of gadd45alpha in carboplatin (carb) or 5-fluorouracil (5-fu)-induced apoptosis in human papillomavirus virus (hpv)-positive hep-2 and hela cells. we report that carb or 5-fu upregulate gadd45alpha and ... | 2007 | 17063488 |
| impact of utilizing p16ink4a immunohistochemistry on estimated performance of three cervical cancer screening tests. | the histopathological diagnosis of cervical intraepithelial neoplasia grade 2,3 (cin 2,3) is subjective and prone to variability. in our study, we analyzed the impact of utilizing a biomarker (p16(ink4a)) together with histopathology to refine the "gold standard" utilized for evaluating the performance of 3 different cervical cancer screening tests: cervical cytology, human papillomavirus (hpv) dna testing and visual inspection with acetic acid (via). cervical biopsies from 2 south african cervi ... | 2007 | 17066437 |
| noninfectious papilloma virus-like particles inhibit hiv-1 replication: implications for immune control of hiv-1 infection by il-27. | human papilloma virus (hpv)-like particles (vlps) have been used as a vaccine to prevent hpv infection. recent studies demonstrate that vlps bind to dendritic cells and induce the expression of antiviral cytokines such as interferon-alpha (ifn-alpha), interleukin-10 (il-10) and ifn-gamma. in the present study, we evaluated the effect of vlps on hiv-1 replication in peripheral blood mononuclear cells (pbmcs), cd4+ t cells, and macrophages. here, we show that vlps suppress the replication of both ... | 2007 | 17068156 |
| nkim-6, a new immortalized human brain capillary endothelial cell line with conserved endothelial characteristics. | primary human brain capillary endothelial cells (hbcecs) are available only in small quantities and have a short life span in vitro; this restricts their use as in vitro model for the blood-brain barrier (bbb). to overcome these limitations, we have established an immortalized hbcec line (nkim-6) by transfection with plxsn16e6e7, which encodes the human papillomavirus type 16 e6 and e7 genes. the cell line exhibits an extended life span in vitro and retains its characteristic endothelial morphol ... | 2007 | 17180596 |
| role of hormone cofactors in the human papillomavirus-induced carcinogenesis of the uterine cervix. | if human papillomavirus (hpv) is necessary for the development of (pre)neoplastic lesions of the uterine cervix, it is not sufficient. among the cofactors involved in the malignant transformation of cells infected by hpv, sex hormones may facilitate the cervical carcinogenesis by different mechanisms, including the induction of squamous metaplasia in the transformation zone of the cervix, interactions between steroid hormones and hpv gene expression and alterations of the local immune microenvir ... | 2007 | 17145130 |
| high-risk human papillomavirus type 16 e7 oncogene associates with cdc25a over-expression in oral squamous cell carcinoma. | cells expressing high-risk human papillomavirus (hpv) e7 protein display impaired checkpoint control after dna damage and exhibit elevated rates of mutagenesis. repression of hpv e7 expression results in the subsequent accumulation of hypophosphorylated retinoblastoma protein and repression of the cdc25a genes. no study has been conducted to elucidate the role of cdc25a in the development and progression of human oral carcinomas. to confirm cdc25a protein expression together with hpv, immunohist ... | 2007 | 17111124 |
| inhibition of growth of cervical cancer cells using a dominant negative estrogen receptor gene. | estrogen stimulates human papilloma virus oncogene expression, promotes cervical cancer (cc) cell proliferation and prevents apoptosis. therefore, blockage of estrogen function may have therapeutic application to cc. | 2007 | 17137618 |
| development of a non-denaturing electrophoresis system for characterization of neutralizing epitopes on hpv virus-like particles. | the precise structure of the hpv16 major neutralizing epitope recognized by h16.v5 monoclonal antibody is unknown. this paper describes a novel polyacrylamide gel electrophoresis (page) for separation of hpv virus-like particles (vlps) using cetyltrimethylammonium chloride (ctac) as a solubilizing agent. ctac page employs koh/ch3co2h (ph 4-5.4) as a buffer system, k+ as the leading ion and 3-aminopropionic acid as a trailing ion. the unique characteristics of a cationic electrophoresis system al ... | 2007 | 17137641 |
| distribution and viral load of eight oncogenic types of human papillomavirus (hpv) and hpv 16 integration status in cervical intraepithelial neoplasia and carcinoma. | current human papillomavirus (hpv) dna testing using pooled probes, although sensitive, lacks specificity in predicting the risk of high-grade cervical intraepithelial neoplasia (cin 2/3) progression. to evaluate selected hpv genotyping, viral load, and viral integration status as potential predictive markers for cin progression, we performed hpv genotyping in formalin-fixed, paraffin-embedded cervical tissue with cervical carcinoma (29 cases) and cins (cin 1, 27 cases; cin 2, 28 cases; cin 3, 3 ... | 2007 | 17192787 |
| distribution of hpv genotypes in 282 women with cervical lesions: evidence for three categories of intraepithelial lesions based on morphology and hpv type. | previously we found differences in the distribution of the individual human papillomavirus types in cervical cancers and high-grade squamous intraepithelial lesions. this suggested that there were differences in risk for progression of high-grade squamous intraepithelial lesions that were related to human papillomavirus type within the category of oncogenic genotypes. in this work, we add additional cases including low-grade squamous intraepithelial lesions. thinprep samples from 282 squamous in ... | 2007 | 17192793 |
| autoclave sterilization of instruments used on women with cervical neoplasia is an effective method of eradicating residual human papillomavirus dna: a polymerase chain reaction-based evaluation. | to determine whether autoclave sterilization eradicates human papillomavirus (hpv) dna on specula and instruments used to treat women with cervical neoplasia. | 2007 | 17194945 |
| an immunohistochemical study of cd1a and cd83-positive infiltrating dendritic cell density in cervical neoplasia. | cervical carcinoma is the second leading cancer in women in malaysia, after breast cancer. human papillomavirus (hpv) has been implicated in the development of dysplasia or cervical intraepithelial neoplasia and progression to squamous cell carcinoma. because of the confinement of the human papillomavirus infection within the epithelial layer, the presence of dentritic cells or langerhans cells in epithelial layer of the ectocervix is paramount in producing immune response. the mature dentritic ... | 2007 | 17197902 |
| high human papillomavirus oncogene mrna expression and not viral dna load is associated with poor prognosis in cervical cancer patients. | cervical cancer is now known to be caused by infection with an oncogenic type of the human papillomavirus (hpv). however, little is known about the continued role of hpv once cancer has been established. here, we describe the quantitative relation between hpv dna copy number and mrna expression of the viral oncogenes (e6 and e7) and the prognostic value of both measures in cervical cancer patients. | 2007 | 17200347 |
| prevalence of human papillomavirus genotypes and their variants in high risk west africa women immigrants in south italy. | the distribution of human papillomaviruses (hpvs) varies greatly across populations and hpv surveys have been performed in different geographical regions in order to apply appropriate vaccine strategies. little information, however, exists regarding hpv genotypes distribution in immigrant women from countries at high incidence for cervical cancer. the aim of this study was to determine the spectrum of hpvs and their variants among hiv-positive and hiv-negative women immigrants in south italy mai ... | 2007 | 17201927 |
| lifetime cigarette smoke and second-hand smoke and cervical intraepithelial neoplasm--a community-based case-control study. | both active cigarette smoking and human papillomavirus (hpv) infection are known risk factors for cervical intraepithelial neoplasm (cin). the association between second-hand smoke (shs) and cin has not been conclusively determined. we conducted a community-based case-control study to estimate the relationship between shs and cin. | 2007 | 17204311 |
| structure-based engineering of papillomavirus major capsid l1: controlling particle assembly. | the outer shell of the papillomavirus particle is comprised of 72 pentamers of the major capsid l1 protein arranged on a t = 7 icosahedral lattice. the recombinant l1 can form t = 7 virus-like particles in vitro. the crystal structure of a t = 7 papilloma virion has not yet been determined; however, the crystal structure of a t = 1 particle containing 12 pentamers is known. the t = 1 structure reveals that helix-helix interactions, through three helices-h2, h3, and h4-near the c-terminus of l1, ... | 2007 | 17210082 |
| changes of epidermodysplasia verruciformis in benign skin lesions: the ev acanthoma. | foci of histological changes of epidermodysplasia verruciformis (ev) were noted in five benign skin lesions. these skin lesions included an intradermal nevus, a pigmented seborrheic keratosis, an isolated papule on the forearm, a perianal lesion, and an acantholytic acanthoma. because the changes resembled true ev so strongly despite the absence of clinical ev in these patients, we searched for ev-human papilloma virus (hpv) types in these skin lesions. polymerase chain reaction (pcr) analysis o ... | 2007 | 17214854 |
| human papillomavirus in normal conjunctival tissue and in conjunctival papilloma: types and frequencies in a large series. | to examine conjunctival papilloma and normal conjunctival tissue for the presence of human papillomavirus (hpv). | 2007 | 17166894 |
| estimating the long-term impact of a prophylactic human papillomavirus 16/18 vaccine on the burden of cervical cancer in the uk. | to predict the public health impact on cervical disease by introducing human papillomavirus (hpv) vaccination in the united kingdom, we developed a mathematical model that can be used to reflect the impact of vaccination in different countries with existing screening programmes. its use is discussed in the context of the united kingdom. the model was calibrated with published data. the impact of vaccination on cervical cancer and deaths, precancerous lesions and screening outcomes were estimated ... | 2007 | 17146475 |
| age-specific detection of high risk hpv dna in cytologically normal, computer-imaged thinprep pap samples. | recent cervical cancer screening guidelines for women over age 30 seek to improve the sensitivity of cytology by incorporating high-risk (hr) human papillomavirus (hpv) dna testing into the screening algorithm, a recommendation based largely on data that utilized the conventional pap smear and were not stratified by patient age. data on the rate of hr hpv among women over age 30 undergoing liquid-based pap test screening are limited. the objective of this study was to determine the rate of hr hp ... | 2007 | 17150245 |
| the nucleotide analog cidofovir suppresses basic fibroblast growth factor (fgf2) expression and signaling and induces apoptosis in fgf2-overexpressing endothelial cells. | cidofovir [(s)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine; (s)-hpmpc] is an antiviral drug that has been approved for the treatment of cytomegalovirus retinitis in patients with aids. cidofovir also possesses potent activity against human papillomavirus-induced tumors in animal models and patients. we have recently shown that cidofovir inhibits the development of vascular tumors induced by basic fibroblast growth factor (fgf2)-overexpressing endothelial cells (fgf2-t-mae) in mice. here, we ... | 2007 | 17158200 |
| new paradigm for prevention of cervical cancer. | human papillomavirus (hpv) infection is an event responsible for the development of cervical cancer and its premalignant dysplasia. prophylactic vaccines based on virus-like particles (vlps) have been successfully tested in clinical trials. they are safe, close to 100% effective in preventing persistent infection and premalignant disease, and are now being introduced onto the market. vaccination should be offered primarily to young girls and adolescents 9-15 years of age. vaccine introduction fa ... | 2007 | 16973254 |
| is there an epidemiological link between human papillomavirus dna and basaloid squamous cell carcinoma of the pharynx? | the epidemiological link between human papillomavirus (hpv) dna and basaloid squamous cell carcinoma (bscc) of the pharynx was studied. the expression of p53 protein was evaluated by immunohistochemical analysis. the presence of hpv dna was evaluated by polymerase chain reaction amplification and "in situ" hybridization. the tobacco and alcohol consumption and the clinical outcomes of nine patients with bscc of the pharynx are compared with site and stage matched 109 conventional squamous cell c ... | 2007 | 16979930 |
| erbb4 (jm-b/cyt-1)-induced expression and phosphorylation of c-jun is abrogated by human papillomavirus type 16 e5 protein. | human papillomavirus type 16 e5 (hpv-16 e5) is a highly hydrophobic membrane protein with weak-transforming activity, which is associated with erbb4 receptor in hpv-16-infected cervical lesions. presently, we investigated the transforming mechanisms of e5 involving erbb4 signaling. firstly, we report a role for erbb4 (jm-b/cyt-1) receptor that activates c-jun gene expression and phosphorylating at ser63 and ser73 of the c-jun protein in ligand-independent and ras-c-jun nh(2)-terminal kinase-depe ... | 2007 | 16819515 |
| thinprep cytology and high-risk human papillomavirus detection on evaluating the efficacy of loop electrosurgical excision procedure in treatment of cervical intraepithelial neoplasia. | 2007 | 16730873 | |
| amino acid substitutions that specifically impair the transcriptional activity of papillomavirus e2 affect binding to the long isoform of brd4. | the e2 protein of papillomaviruses binds to specific sites in the viral genome to regulate its transcription, replication and segregation in mitosis. amino acid substitutions in the transactivation domain (tad) of e2, of arg37 and ile73, have been shown previously to impair the transcriptional activity of the protein but not its ability to support viral dna replication. to understand the biochemical basis of this defect, we have used the tads of a low-risk (hpv11) and a high-risk (hpv31) human p ... | 2007 | 17023018 |
| combined human papillomavirus dna and human papillomavirus-like particle serologic assay to identify women at risk for high-grade cervical intraepithelial neoplasia. | the objective of this study was to assess the utility of a second generation human papillomavirus (hpv) virus-like particle (vlp)-based elisa as an adjunct to hpv dna testing to identify women at risk for high-grade cervical intraepithelial neoplasia (cin). participants provided blood, cervical samples and interviewer-obtained questionnaire information. hpv vlps for types 16, 18, 33, 45 and 52 were produced using a baculovirus expression system. these highly purified vlps were used in a polymer- ... | 2007 | 17036320 |
| exploring the knowledge, attitudes, beliefs, and communication preferences of the general public regarding hpv: findings from cdc focus group research and implications for practice. | genital human papillomavirus (hpv) infection is the most common sexually transmitted virus in the united states, causing genital warts, cervical cell abnormalities, and cervical cancer in women. to inform hpv education efforts, 35 focus groups were conducted with members of the general public, stratified by gender, race/ethnicity, and urban/rural location. focus groups explored participants' knowledge, attitudes, and beliefs about hpv and a hypothetical hpv vaccine as well as their communication ... | 2007 | 17000622 |
| viral oncoproteins target the dna methyltransferases. | small dna tumour viruses have evolved a number of mechanisms to drive nondividing cells into s phase. virally encoded oncoproteins such as adenovirus e1a and human papillomavirus (hpv) e7 can bind an array of cellular proteins to override proliferation arrest. the dna methyltransferase dnmt1 is the major mammalian enzyme responsible for maintaining cpg methylation patterns in the cell following replication. one of the hallmarks of tumour cells is disrupted dna methylation patterns, highlighting ... | 2007 | 16983344 |
| the history of the angiogenic switch concept. | spontaneously arising tumor cells are not usually angiogenic at first. the phenotypic switch to angiogenesis is usually accomplished by a substet that induces new capillaries that then converge toward the tumor. the switch clearly involves more than simple upregulation of angiogenic activity and is thought to be the result of a net balance of positive and negative regulators. tumor growth is although to require disruption of this balance and hence this switch must turned on for cancer progressio ... | 2007 | 16990761 |
| establishment of immortalized dental follicle cells for generating periodontal ligament in vivo. | the dental follicle is a mesenchymal tissue that surrounds the developing tooth germ. during tooth root formation, periodontal components, viz., cementum, periodontal ligament (pdl), and alveolar bone, are created by dental follicle progenitors. here, we report the presence of pdl progenitors in mouse dental follicle (mdf) cells. mdf cells were obtained from mouse incisor tooth germs and immortalized by the expression of a mutant human papilloma virus type 16 e6 gene lacking the pdz-domain-bindi ... | 2007 | 17013589 |
| interferon regulatory factor-1 regulates reconstituted extracellular matrix (recm)-mediated apoptosis in human mammary epithelial cells. | interactions between extracellular matrix (ecm) and mammary epithelial cells are critical for mammary gland homeostasis and apoptotic signaling. interferon regulatory factor-1 (irf-1) is a transcriptional regulator that promotes apoptosis during mammary gland involution and p53-independent apoptosis. we have recently shown that rapid cell surface tamoxifen (tam) signaling promotes apoptosis in normal human mammary epithelial cells that were acutely damaged by expression of human papillomavirus t ... | 2007 | 17016442 |
| hpv16 l1 capsid protein expressed from viable adenovirus recombinants elicits neutralizing antibody in mice. | immunization against human papillomavirus (hpv) infection promises to reduce the worldwide burden of cervical cancer. to evaluate the potential of live recombinant adenoviruses for induction of hpv infection-blocking immunity, we prepared viable adenovirus recombinants that express the hpv16 l1 gene from the adenovirus major late transcriptional unit. adenovirus-produced hpv16 l1 assembles into virus-like particles (vlps) in infected cells in culture. purified hpv16 vlps are recognized by hpv16 ... | 2007 | 16914239 |
| dna viruses in human cancer: an integrated overview on fundamental mechanisms of viral carcinogenesis. | the first experimental data suggesting that neoplasm development in animals might be influenced by infectious agents were published in the early 1900s. however, conclusive evidence that dna viruses play a role in the pathogenesis of some human cancers only emerged in the 1950s, when epstein-barr virus (ebv) was discovered within burkitt lymphoma cells. besides ebv, other dna viruses consistently associated with human cancers are the hepatitis b virus (hbv), human papillomavirus (hpv), and kaposi ... | 2007 | 16814460 |
| human papillomavirus type 77 e7 protein is a weak deregulator of cell cycle. | human papillomavirus type 77 is a skin type found in non-melanoma skin cancers of immuno-compromised individuals. although, the hpv77 e6 oncoprotein has been well studied, nothing is known about e7. studies on mucosal hpv types (e.g. hpv16) showed that e7 deregulates the cell cycle by binding to and promoting degradation of retinoblastoma protein (prb). here, we characterized the impact of hpv77 e7 on the cell cycle. we observed that hpv77 e7 associated with prb with a lower affinity than hpv16 ... | 2007 | 16650526 |
| distribution of human papillomavirus genotypes in the patients with cervical carcinoma and its precursors in zhejiang province, china. | various evidences reveal that the human papillomavirus (hpv) is the single most important etiologic agent in cervical carcinoma (cc). to investigate the distribution of hpv genotypes in the patients with cc and its precursors in zhejiang province, china, a total of 631 eligible samples from patients in zhejiang province with cc (n = 181), cervical intraepithelial neoplasia (cin) ii-iii (n = 345), and cin i (n = 105) were detected. age-matched samples of 217 women without cervical neoplasia were ... | 2008 | 17466048 |
| comparison of the amplicor human papillomavirus test and the hybrid capture 2 assay for detection of high-risk human papillomavirus in women with abnormal pap smear. | infection with human papillomavirus (hpv) is a necessary step in the progression to cervical cancer. many methods for hpv testing are currently available, mostly developed to detect pools of hpv types. hybrid capture 2 (hc2) is one of the most widely used. a new pcr-based assay, the roche amplicor hpv test, has been recently developed. both assays recognize a group of 13 hr hpv types contemporaneously. this study evaluated the performance of both methods for detecting high-grade cervical lesions ... | 2008 | 17854914 |
| hpv triage testing or repeat pap smear for the management of atypical squamous cells (ascus) on pap smear: is there evidence of process utility? | a two-stage standard gamble was used to evaluate women's preferences for alternative managements of atypical squamous cells of undermined significance (ascus) on pap smear (repeat pap smear compared with immediate hpv test), and to test for the evidence of process utility. women's utilities for the health state scenarios were clustered towards the upper end of the 0-1 scale with considerable variability in women's preferences. there was evidence of process utility, with immediate human papilloma ... | 2008 | 17764095 |
| induction of mhc class i molecule cell surface expression and epigenetic activation of antigen-processing machinery components in a murine model for human papilloma virus 16-associated tumours. | epigenetic events play an important role in tumour progression and also contribute to escape of the tumour from immune surveillance. in this study, we investigated the up-regulation of major histocompatibility complex (mhc) class i surface expression on tumour cells by epigenetic mechanisms using a murine tumour cell line expressing human e6 and e7 human papilloma virus 16 (hpv16) oncogenes and deficient in mhc class i expression, as a result of impaired antigen-presenting machinery (apm). treat ... | 2008 | 17725605 |
| p16ink4a expression, human papillomavirus, and survival in head and neck cancer. | development of head and neck cancer (hnc) is associated with human papillomavirus high-risk (hpv-hr) types. the hpv e7 oncoprotein inactivates the prb protein increasing expression of p16(ink4a). p16 expression and hpv status have been associated with differences in clinical outcomes for hnc. this study examined whether hnc prognosis was different when these biomarkers were examined as individual or joint molecular effects. tumor tissue from 301 hnc patients were analyzed and sequenced for hpv t ... | 2008 | 17360226 |
| parental acceptance of human papillomavirus vaccines. | to determine whether parents would accept human papillomavirus (hpv) vaccination for their children and which variables may influence their decision, including knowledge about cervical cancer and hpv. | 2008 | 17368910 |
| age-specific prevalence of high-risk human papillomavirus infection in a hungarian female population with positive cytology. | patients with positive screening results and persistence of high-risk human papillomavirus (hpv) infection represent the population at the highest risk for developing cervical cancer. to describe the epidemiology in this high-risk population, data were collected and analysed at the referral centre for patients with positive cytology. | 2008 | 17714853 |
| human papillomavirus dna versus papanicolaou screening tests for cervical cancer. | 2008 | 18265440 | |
| induction of cytotoxic t-lymphocytes and antitumor activity by a liposomal lipopeptide vaccine. | we have previously described a simple yet effective liposome-based therapeutic vaccine, dotap/e7, which contains only two molecules, the cationic lipid dotap and a peptide antigen derived from the e7 oncoprotein of human papillomavirus (hpv) type 16. in the current report, we have improved the vaccine formulation by incorporation of e7-lipopeptide instead of the water-soluble native e7 peptide into the dotap liposome. the lipopeptide consists of an n-terminal alpha- or -palmitoyl lysine connecte ... | 2008 | 18266319 |
| epidermodysplasia verruciformis with severe hand and foot deformity successfully treated with surgical excision. | epidermodysplasia verruciformis is a rare skin disease due to human papillomavirus (hpv) infection. we report here a male patient of 39 years with severe giant lesion of hands and feet leading to disability for 20 years. the function of the hands was almost lost. we treated the case with the shaving technique and a satisfactory result without recrudescence in the following two years. no apparent scarring resulted. | 2008 | 18267314 |
| improved survival of patients with human papillomavirus-positive head and neck squamous cell carcinoma in a prospective clinical trial. | the improved prognosis for patients with human papillomavirus (hpv)-positive head and neck squamous cell carcinoma (hnscc) relative to hpv-negative hnscc observed in retrospective analyses remains to be confirmed in a prospective clinical trial. | 2008 | 18270337 |
| a novel therapeutic fusion protein vaccine by two different families of heat shock proteins linked with hpv16 e7 generates potent antitumor immunity and antiangiogenesis. | human papillomaviruses (hpv), particularly hpv16, is considered a necessary cause of cervical and oral cancer. thus, the development of a therapeutic vaccine against hpv is important for the control of cervical cancer. however, therapeutic vaccination has been limited by inadequate antigen-specific immune responses. heat shock proteins (hsp), including calreticulin (crt), hsp70 and gp96, have been shown to act as potent immunoadjuvant to enhance antigen-specific tumor immunity. previous studies ... | 2008 | 18272260 |
| imiquimod leads to a decrease of human papillomavirus dna and to a sustained clearance of anal intraepithelial neoplasia in hiv-infected men. | anal intraepithelial neoplasia (ain), a human papillomavirus (hpv)-associated precursor lesion of anal carcinoma, is highly prevalent in hiv-infected men having sex with men (msm). this prospective follow-up study evaluated the long-term results of imiquimod treatment of ain in 19 hiv-infected msm. standardized follow-up examinations included high-resolution anoscopy, anal cytology/histology, hpv typing, and dna load determination for hpv types 16, 18, 31, and 33. mean follow-up time was 30.3 mo ... | 2008 | 18273049 |
| role of il-2 secreted by padre-specific cd4+ t cells in enhancing e7-specific cd8+ t-cell immune responses. | cd4(+) t helper cells are known to play an integral role in the generation of cd8(+) t-cell immune responses. we have previously shown that co-administration of dna vaccines containing e6 or e7 protein of human papillomavirus 16 (hpv-16) combined with dna encoding invariant (ii) chain in which class ii-associated ii peptide (clip) region is replaced with the cd4(+) t helper epitope, padre (pan-dr-epitope) (ii-padre dna) enhanced hpv antigen-specific cd8(+) t-cell immune responses in vaccinated m ... | 2008 | 18273057 |
| age at menarche is not an independent risk factor for high-risk human papillomavirus infections and cervical intraepithelial neoplasia. | data are controversial as to the role of menarche age as a risk factor of high-risk human papillomavirus (hr-hpv) infections. the objective of this study was to analyse the risk estimates for age at menarche as determinant of cervical intraepithelial neoplasia (cin) and hr-hpv infections. a cohort of 3187 women were stratified into three groups according to their age at menarche: (i) women <13 years of age; (ii) those between 13 and 14 years and (iii) women >15 years of age. these groups were an ... | 2008 | 18275641 |
| new viral vaccines for dermatologic disease. | two new viral vaccines have recently been approved by the food and drug administration. human papillomavirus (hpv) vaccine is intended to reduce infection with the most common hpv types that cause anogenital disease, including cervical cancer and genital warts. herpes zoster (hz) vaccine is intended to prevent shingles and its complications. the use of these two vaccines will immediately begin to impact dermatologic practice throughout the world and will reduce the healthcare burden associated w ... | 2008 | 18280332 |
| human papillomavirus vaccination: expected impacts and unresolved issues. | 2008 | 18280831 | |
| the effect of proteasome inhibition on p53 degradation and proliferation in tonsil epithelial cells. | to determine whether proteasome inhibition could reverse e6-mediated p53 degradation, cause selective growth inhibition, and induce apoptosis in human papillomavirus e6-transformed primary tonsil epithelial cells. | 2008 | 18283158 |
| long-term outcomes of high-risk human papillomavirus infection support a long interval of cervical cancer screening. | knowing that infection of high-risk human papillomavirus (hpv) causes virtually all cervical cancer (cc), the long-term outcomes of hpv infection, especially the absolute risk and time lapse of developing cc, are beyond the scope of ordinary follow-up study owing to ethical concerns. the present study followed the natural history and long-term outcomes of hpv infection in a cohort of women by national health insurance care and data linkage without additional disturbance. the status of cervical h ... | 2008 | 18283313 |
| circumcision and human papillomavirus infection in men: a site-specific comparison. | lack of circumcision has been identified as a risk factor for male genital human papillomavirus (hpv) infection, although this association has not been consistently supported. | 2008 | 18284369 |
| a giant condyloma accuminata. | condyloma accuminata is not a rare disease in our country. but such a giant condyloma accuminata is very uncommon case that we reported here. it is a type of venereal wart that commonly grows in genital region, caused by the hpv (human papilloma virus). it is considered one of the most common sexually transmitted disease in western countries. the patient presented with cauliflower like fungating growth over the genitalia for 15 years with pain and burning sensation over the lesion for 1 month. p ... | 2008 | 18285732 |
| a methodological framework to distinguish spectrum effects from spectrum biases and to assess diagnostic and screening test accuracy for patient populations: application to the papanicolaou cervical cancer smear test. | a spectrum effect was defined as differences in the sensitivity or specificity of a diagnostic test according to the patient's characteristics or disease features. a spectrum effect can lead to a spectrum bias when subgroup variations in sensitivity or specificity also affect the likelihood ratios and thus post-test probabilities. we propose and illustrate a methodological framework to distinguish spectrum effects from spectrum biases. | 2008 | 18291032 |
| human papillomavirus and mutated h-ras oncogene in cervical carcinomas and pathological negative pelvic lymph nodes: a retrospective follow-up. | the metastasis status of pelvic lymph nodes (plns) seems to be a predictive factor of survival. it was suggested that the presence of hpv dna and other biological markers in pln may indicate a sub clinical early metastasis. the aim was to describe the prevalence and distribution patterns of hpv dna and h-ras mutations in intra operatively obtained cervical tumors and pln. thirty-seven cervical tumors and 61 lymph node biopsies from 37 patients with cervical cancer were selected. hpv typing and l ... | 2008 | 18297710 |
| frequent epigenetic silencing of the fhit gene in penile squamous cell carcinomas. | methylation of normally unmethylated cpg-rich islands in or near the promoter region has been associated with transcriptional inactivation of tumor-suppressor and tumor-related genes in human cancers. however, so far, only a few studies have searched for dna methylation in penile carcinoma (pc). on the other hand, human papillomavirus (hpv) has been reported to be associated with the pathogenesis of pc. to elucidate the methylation status of pc and hpv infection, the methylation status of eight ... | 2008 | 18299890 |
| hpv vaccine mandates: just say 'no' to the "great big public health experiment". | while many states are seriously considering requiring vaccination of pre-teen girls as a condition of middle school admission, the case for mandatory human papillomavirus (hpv) vaccine is very weak. such a requirement lacks the traditional justification for vaccine mandates and therefore represents an unjustified usurpation ofparental authority. moreover, serious questions remain as to whether the vaccine is effective in preventing cervical cancer. the vaccine is the most expensive pediatric vac ... | 2008 | 18300597 |
| anal intraepithelial neoplasia: correlation of grade with p16ink4a immunohistochemistry and hpv in situ hybridization. | accurate diagnosis and grading of anal intraepithelial neoplasia (ain) can be problematic, especially in separating ain from anal transitional-zone epithelium. immunohistochemistry (ihc) for p16 correlates with human papillomavirus (hpv) integration into the host genome, and hpv subtyping by in situ hybridization (ish) is now readily available. to investigate if p16 would help in more accurately diagnosing and grading ain, particularly when attempting to distinguish benign transitional-zone epit ... | 2008 | 18301250 |
| screening with a primary human papillomavirus test does not increase detection of cervical cancer and intraepithelial neoplasia 3. | to determine cross-sectional validity of primary human papillomavirus (hpv) screening in comparison to cytological screening. | 2008 | 18248809 |
| anomalous features of emt during keratinocyte transformation. | during the evolution of epithelial cancers, cells often lose their characteristic features and acquire a mesenchymal phenotype, in a process known as epithelial-mesenchymal transition (emt). in the present study we followed early stages of keratinocyte transformation by hpv16, and observed diverse cellular changes, associated with emt. we compared primary keratinocytes with early and late passages of hf1 cells, a cell line of hpv16-transformed keratinocytes. we have previously shown that during ... | 2008 | 18253510 |
| pcr detection rates of high risk human papillomavirus dna in paired self-collected urine and cervical scrapes after laser co2 conization for high-grade cervical intraepithelial neoplasia. | to compare the pcr detection rates of high risk human papillomavirus dna in self-collected urine and cervical scrapes during follow-up of patients treated for hg-cin by laser co2 conization. | 2008 | 18255129 |
| hada3 degradation by papillomavirus type 16 e6 correlates with abrogation of the p14arf-p53 pathway and efficient immortalization of human mammary epithelial cells. | two activities of human papillomavirus type 16 e6 (hpv16 e6) are proposed to contribute to the efficient immortalization of human epithelial cells: the degradation of p53 protein and the induction of telomerase. however, the requirement for p53 inactivation has been debated. another e6 target is the hada3 protein, a p53 coactivator and a component of histone acetyltransferase complexes. we have previously described the role of hada3 and p53 acetylation in p14arf-induced human mammary epithelial ... | 2008 | 18256148 |
| human papillomavirus dna versus papanicolaou screening tests for cervical cancer. | 2008 | 18256402 | |
| dna-vaccination via tattooing induces stronger humoral and cellular immune responses than intramuscular delivery supported by molecular adjuvants. | tattooing is one of a number of dna delivery methods which results in an efficient expression of an introduced gene in the epidermal and dermal layers of the skin. the tattoo procedure causes many minor mechanical injuries followed by hemorrhage, necrosis, inflammation and regeneration of the skin and thus non-specifically stimulates the immune system. dna vaccines delivered by tattooing have been shown to induce higher specific humoral and cellular immune responses than intramuscularly injected ... | 2008 | 18257910 |
| p53 codon 72 polymorphism associated with risk of human papillomavirus-associated squamous cell carcinoma of the oropharynx in never-smokers. | the tumor suppressor p53 protein can be bound, degraded and inactivated by the human papillomavirus (hpv) e6 oncoprotein. the p53 protein's susceptibility to this oncoprotein may be influenced by the p53 codon 72 polymorphism, but the role of such a polymorphism in the development of hpv16-associated squamous cell carcinoma of the oropharynx (sccop) has not been established. to investigate the role of the p53 codon 72 polymorphism in the risk of hpv16-associated sccop, we conducted a hospital-ba ... | 2008 | 18258602 |
| comparison of p16ink4a and hybrid capture 2 human papillomavirus testing as adjunctive tests in liquid-based gynecologic surepath preparations. | p16(ink4a), cyclin-dependent kinase inhibitor, is functionally inactivated in many tumors, including cervical cancer. we compared p16(ink4a) immunocytochemical staining and hybrid capture 2 (hcii) on surepath specimens using tissue biopsies (as the gold standard). their utility in a spectrum of atypical and preneoplastic lesions, and their ability to accurately identify underlying lesions of cin ii or greater was assessed using biopsy follow-up data. one-hundred and seventeen residual surepath s ... | 2008 | 18232003 |
| [effect of over expression of hpv18 e2 protein on apoptosis and secretion of macrophages]. | to study the effect of the over expression of gfp-e2, gfp-tad(n-extremity domain of hpv18 e2) and gfp-dbd (c-extremity domain of hpv18 e2) on the apoptosis and secretion of macrophages and to further explore the contribution of e2 gene to the uterine cervix cancer. | 2008 | 18237530 |
| regulation of human papillomavirus type 31 gene expression during the differentiation-dependent life cycle through histone modifications and transcription factor binding. | the life cycle of high-risk human papillomaviruses is linked to epithelial differentiation with virion production restricted to highly differentiated suprabasal cells. two major viral promoters direct high-risk hpv gene expression and their activities are dependent upon differentiation. the early promoter controls initiation of transcripts at sites upstream of the e6 open reading frame and is active in both undifferentiated as well as differentiated cells. the late viral promoter directs transcr ... | 2008 | 18237759 |
| human papillomavirus-related content in state and tribal comprehensive cancer control plans. | oncogenic types of the human papillomavirus (hpv) are firmly established as etiological agents for most premalignant and malignant epithelial lesions of the cervical mucosa. genital infection with hpv is the most common sexually transmitted infection (sti) in the united states. although most women infected with the virus become hpv negative within 2 years, women with persistent high-risk hpv infections are at greatest risk for developing cervical cancer. since the development of the papanicolau ... | 2008 | 18240976 |
| effects of cellular differentiation, chromosomal integration and 5-aza-2'-deoxycytidine treatment on human papillomavirus-16 dna methylation in cultured cell lines. | human papillomavirus-16 (hpv-16) genomes in cell culture and in situ are affected by polymorphic methylation patterns, which can repress the viral transcription. in order to understand some of the underlying mechanisms, we investigated changes of the methylation of hpv-16 dna in cell cultures in response to cellular differentiation, to recombination with cellular dna, and to an inhibitor of methylation. undifferentiated w12e cells, derived from a precancerous lesion, contained extrachromosomal h ... | 2008 | 18242658 |
| [evaluation of accuracy of three assays for human papillomavirus detection and typing: hybrid capture 2, hpv consensus kit and amplicor hpv]. | detection of high-risk human papillomavirus has proved its usefulness in complement of abnormal cervical scrape result. the hybrid capture 2 (hc2, digene) test has proven its efficiency. we have compared this test with hpv consensus kit (hpvc, argène) and amplicor hpv test (ahpv, rochediagnostics) on a panel of 88 samples with low hc2 ratios or discordant results between hc2 and cervical scrape. | 2008 | 18178026 |
| human papilloma virus (hpv) status, p16ink4a, and p53 overexpression in epithelial malignant and borderline ovarian neoplasms. | this investigation is the first to evaluate simultaneously human papilloma virus (hpv) status, p16(ink4a), and p53 immunoreactivity in epithelial ovarian neoplasms. the results were analyzed and correlated with histological type, histological grade, and survival of patients. subtypes considered are papillary serous and mucinous. polymerase chain reaction (pcr) analysis, performed in our previous study, had already demonstrated a small number of hpv-positive epithelial ovarian neoplasms. no signi ... | 2008 | 18180113 |
| survey hints at uneven adoption of cancer vaccine. | 2008 | 18180705 | |
| regulation of telomerase and telomeres: human tumor viruses take control. | human tumor viruses are responsible for one-fifth of all cancers worldwide. these viruses have evolved multiple strategies to evade immune defenses and to persist in the host by establishing a latent infection. proliferation is necessary for pretumor cells to accumulate genetic alterations and to acquire a transformed phenotype. however, each cell division is associated with a progressive shortening of the telomeres, which can suppress tumor development by initiating senescence and irreversible ... | 2008 | 18182620 |
| transcriptional regulation of human papillomavirus type 18 p105 promoter by the co-activator cbp. | human papillomaviruses (hpvs) are the etiological agents of cervical cancer, with hpv-16 and 18 being the representative types of the higher risk group. the expression of the viral genes with transforming activity (e6 and e7) is controlled by the upstream regulatory region (urr), a segment of the viral genome that contains elements recognized by several transcription factors. | 2008 | 18182775 |
| hpv and cervical cancer: screening or vaccination? | following the demonstration of the superior validity of human papillomavirus (hpv) tests in screening for cervical cancer and the arrival of highly efficacious hpv 16 and 18 vaccines, cervical cancer prevention enters a time of sustainable introduction in developing countries. multidisciplinary efforts and novel protocols are being developed, and challenging situations are being faced to make cervical cancer, still the number two cancer in women worldwide, an eradicable condition. | 2008 | 18182975 |
| down-regulation of transforming growth factor-beta type ii receptor (tgf-betarii) protein and mrna expression in cervical cancer. | cervical carcinogenesis is a multistep process initiated by "high risk" human papillomaviruses (hr-hpv), most commonly hpv16. the infection per se is, however, not sufficient to induce malignant conversion. transforming growth factor beta (tgf-beta) inhibits epithelial proliferation and altered expression of tgf-beta or its receptors may be important in carcinogenesis. one cofactor candidate to initiate neoplasia in cervical cancer is the prolonged exposure to sex hormones. interestingly, previo ... | 2008 | 18184435 |
| placental infection with human papillomavirus is associated with spontaneous preterm delivery. | we sought to determine if human papillomavirus (hpv) infection of extravillous trophoblast cells reduces cell invasion and if placental infection is associated with adverse reproductive outcomes attributed to placental dysfunction. | 2008 | 18184644 |
| detection of human papillomavirus dna in cutaneous squamous cell carcinoma among immunocompetent individuals. | the presence of certain types of human papillomavirus (hpv) is a known risk factor for the development of anogenital squamous cell carcinomas (sccs). a similar association has been hypothesized for cutaneous sccs, although, to our knowledge, no studies to date have combined sensitive hpv dna detection techniques with epidemiologic data controlling for known risk factors to explore the association. we designed a case-control study examining hpv prevalence using highly sensitive pcr-detection assa ... | 2008 | 18185530 |
| evaluation of p16ink4a as a diagnostic tool in the triage of pap smears demonstrating atypical squamous cells of undetermined significance. | p16(ink4a) (p16) has emerged as a biomarker for the detection of high-risk human papillomavirus (hr-hpv) in papanicolaou (pap) smears. many studies have confirmed a strong correlation between p16 immunohistochemical positivity and high-grade squamous intraepithelial lesions (hsil) of the cervix. because p16 is predictive of hr-hpv and hsil, it seems plausible that p16 could be used as a diagnostic tool to triage atypical squamous cells of undetermined significance (ascus) pap smears. in this way ... | 2008 | 18186493 |