Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| senescence of a human lymphoblastoid clone producing anti-rhesus(d). | the exploitation of epstein-barr virus transformation to generate human lymphoblastoid clones (lcl) producing antibody of predefined specificity has proved highly inefficient. observations reported here on a cloned lcl producing anti-rhesus(d) may provide an explanation for the low success rate. over a few months this clone manifested a progressive loss of capacity to maintain growth in culture. evidence consistent with terminal differentiation to a cell with the properties of a nonproliferating ... | 1985 | 3924635 |
| the b cell surface molecule b1 is functionally linked with b cell activation and differentiation. | the b1 molecule is a 32,000 m.w. phosphorylated cell surface protein expressed exclusively by b cells from the mid pre-b until the plasma cell stage of differentiation. two monoclonal antibodies (gamma 2a and mu) reactive with this molecule were used to assess the role of b1 in b cell activation, proliferation, and differentiation. the anti-b1 antibodies at concentrations ranging from 0.1 to 100 micrograms/ml significantly inhibited b cell proliferation induced by anti-mu antibodies, staphylococ ... | 1985 | 3925015 |
| establishment of two new myeloma cell lines from bilateral pleural effusions: evidence for sequential in vivo clonal change. | two new human myeloma cell lines have been established from a 36-year-old woman with refractory igg kappa multiple myeloma in whom bilateral malignant pleural effusions developed. the malignant plasma cells from each effusion were set up in a liquid culture using an l-15 medium containing catalase, glutathione, selenous acid, ascorbic acid, insulin, transferrin, additional glutamine hydrocortisone, and 2-mercaptoethanol and designated as m-3 medium. two igg kappa cell lines, lb -831 and lb-832, ... | 1985 | 3927997 |
| a newly established human lymphoma cell line, fl-18, carrying a 14;18 translocation. | a new human cell line named fl-18, carrying a 14;18 translocation [t(14;18)(q32;q21)], was established from a japanese patient with follicular small cleaved cell lymphoma. the established fl-18 cells had monoclonal surface immunoglobulins (igg-kappa) and were negative for epstein-barr virus nuclear antigens. the high-resolution banding technique indicated the breakpoints of chromosomes 14 and 18 involved in the characteristic 14;18 translocation to be at sub-bands 14q32.3 and 18q21.3, respective ... | 1985 | 3928551 |
| induction of cell differentiation in burkitt lymphoma lines. bla: a glycolipid marker of b-cell differentiation. | we have previously described an mab referred to as 38.13 which reacts with a glycolipid membrane antigen (named bla) on burkitt lymphoma (bl) lines. the bla antigen and other b-cell differentiation markers have been studied on bl lines treated with sodium butyrate, agents from the phorbol-diester series (with or without differentiating properties) and teleocidin. with all differentiation inducers tested, expression of bla as well as of surface igm decreased on the induced cells whereas that of b ... | 1985 | 3930411 |
| delineation of functional sites in hla-b27 antigens. molecular analysis of hla-b27 variant wewak i defined by cytolytic t lymphocytes. | an hla-b27 positive, epstein barr virus-transformed cell line wewak i was not lysed by epstein barr virus-specific cytolytic t lymphocytes restricted by hla-b27. this line is weakly reactive with a b27-specific monoclonal antibody, m2, which recognizes a majority, but not all, of the hla-b27-positive cells. to establish the molecular basis for this lack of recognition, the structure of the variant hla-b27 antigen was compared with the known structure of hla-b27 from the lg-2 cell line, which is ... | 1985 | 3930603 |
| [production of human monoclonal antibodies against various antigens of erythrocyte stages of plasmodium falciparum]. | peripheral blood lymphocytes from 20 individuals living in a malaria endemic area (burkina faso) were transformed with epstein-barr virus. no antigen specific selection, nor stimulation of b-cells were performed prior to transformation. 20 cell lines were established, 14 secreted polyclonal antibodies directed against erythrocytic stages of plasmodium falciparum. 4 lines were cloned and the supernatant analysed and characterised against plasmodium falciparum antigens. | 1985 | 3933777 |
| effects of tumor promoters on the expression of a tumor-related multigenic set in human cells. | we previously found that a minor subfraction of the human genomic dna, corresponding to 2500-3000 nonrepetitive sequences of 3 kilobases each and designated as tumor-activated dna (tadna) was transcriptionally active in burkitt's lymphoma cells and almost inactive in normal lymphocytes growing in vitro following integration of the epstein-barr virus genome. furthermore all the neoplastic cells in culture or primary neoplasms (leukemias, sarcomas, carcinomas) studied contained transcripts from mo ... | 1985 | 3933820 |
| the use of lymphomatous and lymphoblastoid cell lines in the study of burkitt's lymphoma. | to better characterize the virological and cytogenetic features of burkitt's lymphoma (bl) occurring in so-called low-incidence areas, biopsy specimens were collected from bl patients diagnosed and treated in france, and attempts were made to cultivate malignant cells in vitro in order to provide large amounts of tumour material for further laboratory investigations. sixty new bl-derived lymphomatous cell lines have been established from 43 individuals: 24 caucasians, 14 north africans and five ... | 1985 | 3934070 |
| demonstration of 4-aminobutyric acid aminotransferase deficiency in lymphocytes and lymphoblasts. | lysates of lymphocytes, isolated from whole blood, and epstein-barr virus transformed cultured lymphoblasts catalysed the transamination of 4-aminobutyric acid with 2-oxoglutaric acid as co-substrate. 4-aminobutyric acid aminotransferase activity in lymphocyte and lymphoblast sonicates derived from 12 unrelated control individuals (6 each) was 39 +/- 19 pmol min(-1) (mg protein (-1] (mean +/- 1 sd). activities in lysates of both types of cell derived from a flemish patient were less than 3% of c ... | 1985 | 3939544 |
| [the ebv virus: infection, multiplication and carcinogenesis]. | 1985 | 3036674 | |
| quantitative analysis of immunosuppression in cyclosporine-treated heart transplant patients with lymphoma. | lymphoma remains an important complication after heart transplantation in the era of cyclosporine immunosuppression. it has been generally assumed that the occurrence of lymphoma related to quantitative degree of immunosuppression, and this assumption is exemplified in the treatment of some such lymphomas with reduction of immunosuppression. seventy-five consecutive survivors of heart and heart-lung transplantation between december 1980 and july 1983 were treated with cyclosporine and steroids; ... | 1985 | 3039097 |
| lymphocyte function in human bone marrow. i. characterization of two t cell populations regulating immunoglobulin secretion. | we analyzed the regulation of immunoglobulin (ig) production in short-term cultures of human (rib) bone marrow cells. in contrast to blood or tonsil cell cultures, large quantities of igg and iga, but not igm, were secreted by unstimulated marrow cells. the addition of pokeweed mitogen or phytohemagglutinin resulted in the suppression of this ig secretion. both mitogens induced the production of high levels of interleukin 2 (il 2) in marrow cultures, and the addition of il 2 alone mimicked the s ... | 1985 | 3156927 |
| production and characterization of a monoclonal antibody that binds reed-sternberg cells. | a murine monoclonal antibody, termed hefi-1, was produced after immunization with the l428 hodgkin's disease tissue culture cell line. hefi-1 selectively stained only the reed-sternberg or hodgkin's cells in 18 of 18 cases of hodgkin's disease, including the nodular sclerosis, mixed cellularity, and lymphocyte-depleted histologic subtypes. hefi-1 did not stain any cells in normal lung, brain, salivary gland, thyroid, gall bladder, pancreas, liver, testis, breast, endometrium, or kidney. rare lar ... | 1985 | 3157756 |
| antigen-specific interaction between t and b cells. | it is well known that b cells require t-cell help to produce specific antibody. classic experiments suggested that antigen-specific helper t cells interact with antigen-specific b cells via an antigen 'bridge', the b cells binding to one determinant on an antigen molecule (the 'hapten'), while the t cells at the same time recognize another determinant (the 'carrier'). t-helper cells bind specifically to antigen-presenting cells (apc), which have picked up and processed the appropriate antigen, a ... | 1985 | 3157869 |
| regulation of the growth of epstein-barr virus-infected b cells. i. growth regression by e rosetting cells from vca-positive donors is a combined effect of autologous mixed leukocyte reaction and activation of t8+ memory cells. | regression of b cell proliferation in co-cultures of ebv-infected b cells (bebv) and autologous t cells at 1:4 ratio was studied. 3h-tdr incorporation was used to measure proliferation by the participating lymphocyte populations and a 51cr release assay was used to document the generation of cells capable of killing autologous ebv-transformed b lymphoblastoid cell lines (lclebv). ebv-infected b cells cultured alone transformed to blasts by culture day 10, and continued to proliferate throughout ... | 1985 | 2857749 |
| evidence that respiratory tract is major reservoir for epstein-barr virus. | exfoliated cells harvested from bronchial washings of 53 patients with suspected bronchogenic carcinoma were tested by means of dna dot hybridisation using the cloned large internal repeat (ir) sequence of epstein-barr virus (ebv) genome as a probe. 25 of these patients gave positive results. since the patients had diseases that were not related to the virus, this finding suggests that the lower respiratory tract is a major reservoir for ebv. attempts at cellular localisation of the virus reveal ... | 1985 | 2858744 |
| ebv and persistent malaise. | 1985 | 2859466 | |
| infectious mononucleosis mimicking a b cell immunoblastic lymphoma associated with an abnormality in regulatory t cells. | an elderly woman is described with infectious mononucleosis in whom cervical node biopsy was interpreted as showing immunoblastic lymphoma. concomitant reactive lymphocytosis, epstein-barr virus serologic results consistent with an acute infection, and demonstration of polyclonal b cell infiltration of other tissues argued against intervention. defective in vitro t cell responses were demonstrated during the acute phase of epstein-barr virus infection. infectious mononucleosis has rarely been re ... | 1985 | 2859806 |
| effect of n-butyrate on superinfection of raji lymphoblastoid cell line by epstein-barr virus. | the effect of n-butyrate on superinfectability of virus-nonproducer raji cells by the p3hr-1 strain of epstein-barr virus (ebv) was investigated. n-butyrate is known to be a potent inducer of virus antigen synthesis in virus-producer cell lines and of cell differentiation in virus nonproducers. the drug inhibited the growth of raji cells but did not interfere markedly with cell viability. it induced a low rate of early antigen (ea) synthesis in about 1-2% of noninfected raji cells. while the num ... | 1985 | 2860802 |
| epstein-barr virus and persistent malaise. | 1985 | 2861520 | |
| immunoprecipitation of epstein-barr virus (ebv)-specific proteins by prelymphomatous and normal baboon sera containing antibodies reactive with ebv early antigen. | carbon (14c)-amino acid mixture-labelled antigen extracted from 12-0-tetradecanoyl-phorbol-13-acetate (tpa) and sodium n-butyrate-induced p3hr-1 cells was immunoprecipitated with sera from normal, prelymphomatous and lymphomatous baboons, as well as with sera from human controls. most of the epstein-barr virus (ebv)-specific proteins that were precipitated by human sera were also precipitated by baboon sera. a major difference between prelymphomatous and normal control baboon sera was that 5 out ... | 1985 | 2864826 |
| opportunistic lymphoproliferations associated with epstein-barr viral dna in infants and children with aids. | two types of lymphoproliferative disease associated with epstein-barr viral (ebv) dna--central-nervous-system lymphoma and chronic lymphocytic interstitial pneumonitis (lip)--were recognised in children with human t-lymphotropic virus type iii/lymphadenopathy virus infection and the acquired immunodeficiency syndrome (aids). eight of ten lung biopsy specimens from children with lip contained ebv dna. ebv dna was not identified in lung biopsy specimens with pneumocystis carinii, cytomegalovirus, ... | 1985 | 2867394 |
| the oestrogen antagonists, tamoxifen and fc-1157a, display oestrogen like effects on human lymphocyte functions in vitro. | we studied the in vitro effects of two anti-oestrogens (tamoxifen and fc-1157a) on human lymphocyte functions in pokeweed mitogen (pwm) stimulated cultures. addition of 1-100 nmol/l of tamoxifen or fc-1157a to pwm cultures significantly increased the number of immunoglobulin secreting cells. this concentration of tamoxifen did not affect the proliferative response. further experiments with fractionated lymphocyte subpopulations showed that the anti-oestrogens displayed their enhancing effect by ... | 1985 | 2931228 |
| traumatic (eosinophilic) granuloma of oral soft tissues: a report of two cases with pseudo-lymphomatous features. | two cases originally diagnosed as extra-nodal lymphomas occurring in lingual and buccal mucosa which subsequently were reclassified as variants of traumatic granuloma are reported. one case may have been associated with epstein-barr virus infection. extra-nodal lymphoma in such sites is very uncommon and the possibility of traumatic granuloma with a pseudo-lymphomatous appearance must be considered when making such a diagnosis. | 1985 | 2932147 |
| morbidity of cytomegalovirus infection in recipients of heart or heart-lung transplants who received cyclosporine. | forty-four heart and five heart-lung transplant recipients with cytomegalovirus (cmv) infection were investigated for risk factors associated with symptomatic cmv infection (17 patients) and cmv pneumonia (eight patients). symptomatic infection was associated with primary rather than reactivated infection (p less than .005), younger age (p less than .005), heart-lung transplantation (p less than .001), and significant rises in titer of antibody to the early antigen of epstein-barr virus (p less ... | 1985 | 2999255 |
| development of large granular lymphocytes in owl monkeys following ebv inoculation. | 1985 | 2999264 | |
| immune response to epstein-barr virus (ebv) in ataxia-telangiectasia: ebv-specific antibody patterns and their relation to cell-mediated immunity. | epstein-barr virus (ebv)-specific antibody titers were investigated in 60 patients with ataxia-telangiectasia (at) and 22 healthy members of their families. in addition, we studied 36 patients with primary immunodeficiencies, behçet disease, and other conditions and 61 unrelated healthy controls. twenty-seven at patients were examined sequentially at intervals varying from 2 months to 8 years. the at patients showed an increased incidence (66.6%) of high antibody titers (greater than or equal to ... | 1985 | 2999343 |
| defective allosuppression in patients with ataxia-telangiectasia. | we describe a novel in vitro assay system that detects the generation of suppressor t cells after exposure of human lymphocytes to class i alloantigens. we have used this system to study immune functions in a group of seven patients with ataxia-telangiectasia (at). normal t lymphocytes exposed to cells differing at the a and b locus histocompatibility locus antigens (hla) become activated for suppression of epstein-barr virus (ebv)-induced immunoglobulin (ig) production. in contrast to the norma ... | 1985 | 2999344 |
| construction and analysis of additional adenovirus substitution mutants confirm the complementation of vai rna function by two small rnas encoded by epstein-barr virus. | adenovirus vai rna is essential for the efficient initiation of translation of viral mrnas at late times after infection. recently, by constructing an adenovirus type 5 substitution mutant, we showed that the epstein-barr virus encoded two small rnas complemented for the vai rna function in the adenovirus type 5 lytic growth (bhat and thimmappaya, proc. natl. acad. sci. usa 80:4789-4793, 1983). this observation was based on our inability to propagate an adenovirus type 5 mutant lacking functiona ... | 1985 | 2999431 |
| epstein-barr virus genome may encode a protein showing significant amino acid and predicted secondary structure homology with glycoprotein b of herpes simplex virus 1. | we report significant sequence and predicted secondary structure homology between the herpes simplex virus 1 glycoprotein b (gb) and a protein predicted to be encoded by the balf4 reading frame of epstein-barr virus (ebv). homology was detectable at the dna level and was highly significant at the protein level and when evolutionary substitution frequencies of amino acids in related proteins were taken into account. hydropathic analyses predicted that the two proteins possess conserved n-terminal ... | 1985 | 2999435 |
| localization of the coding region for an epstein-barr virus early antigen and inducible expression of this 60-kilodalton nuclear protein in transfected fibroblast cell lines. | expression of a component of the epstein-barr virus early antigen (ea) complex has been studied in fibroblast cells transfected with both wild-type and p3hr-1 defective dna fragments covering the bamhi-m-s region of the epstein-barr virus genome. baby hamster kidney (bhk) cells transfected with the bglii-j fragment and stained with human serum that was positive for the diffuse component of ea [ea(d)] in an indirect immunofluorescence assay exhibited positive nuclear staining in 5% of the cell po ... | 1985 | 2999441 |
| a second epstein-barr virus early antigen gene in bamhi fragment m encodes a 48- to 50-kilodalton nuclear protein. | we used antiserum raised against the bacterially synthesized product of one of the open reading frames in epstein-barr virus (ebv) bamhi fragment m to demonstrate that this reading frame (bmrf1) codes for a nuclear protein of the diffuse early antigen (ea) class. in indirect immunofluorescence assays, the rabbit anti-bmrf1 antiserum gave nuclear staining in approximately 5% of raji cells which had been treated with sodium butyrate, and positive fluorescence was observed in both acetone- and meth ... | 1985 | 2999442 |
| structure and evolution of two related transcription units of epstein-barr virus carrying small tandem repeats. | two regions of the epstein-barr virus (ebv) genome carrying partially homologous clusters of short tandem repeats (noti and psti repeats) flanked by 1044 and 1045 base pairs with almost complete homology (dl and dr, left and right duplication, respectively) were most abundantly transcribed into poly(a)+ mrna after induction with the tumor promoter 12-o-tetradecanoyl-phorbol-13-acetate. the nucleotide sequence of both repeat clusters and the conserved upstream regulatory sequences from the m-aba ... | 1985 | 2999451 |
| [retinitis in a female patient with a prolonged febrile condition caused by epstein-barr viruses]. | 1985 | 2999535 | |
| burkitt's and other non-hodgkin's lymphomas in adults exposed to a visitor from africa. | 1985 | 2999593 | |
| replication of epstein-barr virus within the epithelial cells of oral "hairy" leukoplakia, an aids-associated lesion. | we conducted a study to identify the viruses in tissue specimens of oral "hairy" leukoplakia, a lesion that is found in immunosuppressed male homosexuals and that is associated with the subsequent development of the acquired immunodeficiency syndrome. when stained for papillomavirus core antigen, 49 of 67 biopsy specimens (73 per cent) yielded positive results in epithelial-cell nuclei. electron microscopy showed papillomavirus-like particles in all of 25 specimens, and the herpes-type virus des ... | 1985 | 2999595 |
| epstein-barr virus. dream or reality of a vaccine? | 1985 | 2999603 | |
| protection of cottontop tamarins against epstein-barr virus-induced malignant lymphoma by a prototype subunit vaccine. | epstein-barr (eb) virus is one of the five herpesviruses of man. strong links between this agent and the chain of events causing two human cancers, endemic burkitt's lymphoma and undifferentiated nasopharyngeal carcinoma, have long been evident (reviewed in ref. 1). because of this, and because of the very high incidence of nasopharyngeal carcinoma in certain large populations, it was suggested in 1976 that a vaccine should be developed against eb virus to prevent infection and thereby reduce tu ... | 1985 | 2999604 |
| dna sequence of the region in the genome of herpes simplex virus type 1 containing the genes for dna polymerase and the major dna binding protein. | in the long unique region of the genome of herpes simplex virus type 1 (hsv-1), the genes for dna polymerase and the major dna binding protein are arranged in a head to head manner, with an origin of dna replication (termed oril) located between them. this paper reports an 8400 base pair dna sequence containing both genes and the origin, obtained mostly by m13/dideoxy analysis of plasmid cloned fragments. amino acid sequences of the two proteins were deduced. homologues of both genes were detect ... | 1985 | 2999714 |
| sequence and mapping analyses of the herpes simplex virus dna polymerase gene predict a c-terminal substrate binding domain. | the herpes simplex virus dna polymerase provides an excellent model for studies of eukaryotic replicative polymerases. we report here the nucleotide sequence of the gene which encodes this enzyme. the gene includes a 3705-base-pair major open reading frame capable of encoding a mr 136,519 polypeptide, in rough agreement with previous estimates of the size of the major polypeptide found in partially purified viral polymerase preparations. the predicted polymerase polypeptide shares extensive sequ ... | 1985 | 2999787 |
| cellular and viral dna hypomethylation associated with induction of epstein-barr virus lytic cycle. | epstein-barr virus (ebv) producer and nonproducer cell lines have been treated with a combination of phorbol 12-myristate 13-acetate and n-butyrate (sodium salt). these inducers caused a massive hypomethylation of the ebv producer line p3hr-1 dna (about 30%) at the time when dna replication was inhibited. the viral dna in these cells is heavily methylated as judged by digestion with hpa ii and probing with the bam hi h fragment of ebv. however, upon induction with phorbol 12-myristate 13-acetate ... | 1985 | 2999791 |
| influence of the human t-lymphotropic virus/lymphadenopathy-associated virus on functions of human lymphocytes: evidence for immunosuppressive effects and polyclonal b-cell activation by banded viral preparations. | the etiologic agent for the acquired immunodeficiency syndrome (aids) is now firmly established as the retrovirus termed the human t-lymphotropic virus type iii (htlv-iii) or the lymphadenopathy-associated virus, lav. the disease is characterized by profound and progressive loss of immunity, but molecular evidence indicates that only a few cells in peripheral blood are being productively infected with this virus. in the present study we have investigated a disrupted htlv-iii viral preparation fo ... | 1985 | 2999797 |
| synthesis of rheumatoid factor in vitro: implications for the pathogenesis of rheumatoid arthritis. | 1985 | 2999987 | |
| [monkey b-lymphocyte subpopulations transformed by baboon herpes virus in vivo and in tissue cultures]. | baboon herpes virus (bhv) similarly to human epstein-barr virus shows tropism to b cell subpopulation. in lymphoblastoid cultures transformed in vitro by bhv no e-rfc (t cells) are found but there are b cells with surface and cytoplasmic immunoglobulins. in a number of cultures igm could be detected only in the cell cytoplasm. e-rfc are detectable in cultures in early passages but not after 17 passages which may be due to their elimination. the time course of cell-mediated immunity values was st ... | 1985 | 3000082 |
| infection of human epithelial cells by epstein-barr virus (ebv). ii. biochemical characterization of ebv-determined proteins synthesized in epithelial cells. | primary cultures of epithelial cells were grown from tonsils of patients with diseases not related to ebv. the cells were implanted with ebv receptors and exposed to ebv of the transforming (b95-8, ag-876) and nontransforming (p3hr-1) strains. the ebv-infected and control cells were pulsed with [35s]methionine at 18-24 h after infection, and cell extracts were prepared for immunoprecipitation with anti-ebv sera and analysis by gel electrophoresis and autoradiography. about 20 ebv-determined prot ... | 1985 | 3000099 |
| [cholesterol pneumopathy in children. apropos of 3 cases. possible role of chronic epstein-barr virus infection]. | three cases of cholesterol interstitial pneumonia in patients 3, 9 and 10 years of age respectively are reported. all three were born in island of reunion. two were sisters. all had failure to thrive, dyspnea on rest and clubbing. respiratory symptoms had appeared early in infancy. open pulmonary biopsy was diagnostic. prognosis was poor the boy dying at 4 years of age and severe respiratory insufficiency at 9 or 10 years in the two girls. current etiological investigations were non contributory ... | 1985 | 3000313 |
| a method for analysing case-control studies with ordinal exposure variables. | a method is proposed for analysing case-control studies with ordinal or continuous, but unruly, exposure levels. a test is proposed which is an appropriate linear combination of stratum-specific wilcoxon tests. an estimator for the mean percentile shift between the case and control exposure levels is given. methods for assessing heterogeneity of the mean percentile shift across strata are also discussed. the performance of the test as a function of the number of controls per case is studied for ... | 1985 | 3000467 |
| an ebv membrane protein expressed in immortalized lymphocytes transforms established rodent cells. | epstein-barr virus expresses a cytoplasmic and plasma membrane protein (lmp) in latently infected growth transformed lymphocytes. the gene specifying lmp has now been expressed in nih3t3 and rat-1 cells. expression of the gene in these cells resulted in altered cell morphology and some resistance to the growth inhibiting effect of medium containing low serum. in rat-1 cells, lmp expression often led to loss of contact inhibition and anchorage-independent growth in soft agar. rat-1 cells expressi ... | 1985 | 3000618 |
| subsets of t lymphocytes in relation to t lymphocyte function in multiple sclerosis. | t lymphocyte control of epstein-barr virus (ebv) infection of autologous b lymphocytes was examined in parallel to the enumeration of subpopulations of mononuclear cells in 22 multiple sclerosis (ms) patients and in 22 healthy individuals. all were seropositive for ebv. the incidence of lack of t cell control was significantly higher in patients than in controls, confirming previous published work. in the present study, we have shown in addition a significantly reduced proportion of okt8+ cells ... | 1985 | 3000660 |
| does cytomegalovirus play a role in community-acquired pneumonia? | cytomegalovirus (cmv) is recognized as an important pathogen in the immuno-suppressed patient. sporadic case reports of cytomegalovirus community-acquired pneumonia have appeared. we studied 443 patients with community-acquired pneumonia requiring hospitalization to define the role of cytomegalovirus in this illness. four patients (0.9%) had good evidence that cytomegalovirus caused their pneumonia: 2 had the virus isolated from pulmonary tissue and 2 had cytomegalovirus inclusion bodies visuali ... | 1985 | 3000667 |
| epstein-barr virus in rheumatoid arthritis. | 1985 | 3000687 | |
| beta-adrenergic-stimulated adenylate cyclase activity in normal and ebv-transformed lymphocytes. | beta-adrenergic-associated cyclic amp accumulation was studied in intact lymphocytes before and after transformation with epstein-barr virus into immortal cell lines. although a marked reduction in isoproterenol-stimulated cyclic amp synthesis was observed in transformed cells, forskolin-stimulated cyclic amp accumulation was preserved. a parallel loss of 125-iodocyanopindolol binding sites suggests that the reduction in beta-adrenergic-stimulated amp synthesis is due to receptor down-regulation ... | 1985 | 3000812 |
| [analysis of epstein-barr virus transformation of human lymphocytes: susceptibility of b lymphocyte subpopulations and differentiation stage of the transformed cells]. | the subpopulations of b lymphocytes in human adult peripheral blood which are susceptible to infection and transformation by epstein-barr virus (ebv), and also the stage of ebv-transformed cells in b cell differentiation lineage were identified. the ebv susceptibility were analyzed by two-color immunofluorescence and colony formation in semisolid agar. surface ig-bearing lymphocytes of 3 major ig classes, igm, igg, and iga, in peripheral blood expressed viral nuclear antigen (ebna) after exposur ... | 1985 | 3000906 |
| a modified immunofluorescence test for epstein-barr virus-specific igm antibody. | the fluorescent antibody (fa) test for epstein-barr virus (ebv)-specific igm antibody was improved by the use of sodium butyrate to induce a higher level of ebv antigen expression in p3hr-1 slide preparations and by removal of rheumatoid factor (rf) and igg antibodies from test sera by means of adsorption with suspensions of sepharose-igg and streptococcus pyogenes strain ar1. this method was compared with the paul-bunnell test (pb) on 1106 sera submitted to a routine virus diagnostic laboratory ... | 1985 | 3001120 |
| advances in the diagnosis and treatment of ebv-associated lymphoproliferative diseases in immunocompromised hosts. | the clinical, immunopathologic, and virologic features of the lymphoproliferative diseases occurring after renal transplantation have been characterized. clinically, patients may present with an infectious mononucleosis-like illness or with localized solid tumor masses. these lymphoproliferative diseases have unique histologic features that can be classified as polymorphic diffuse b-cell hyperplasia (pdbh) or polymorphic b-cell lymphoma (pbl). immunologic cell-typing studies have shown that the ... | 1985 | 3001429 |
| htlv-i infection of t and b cells of a patient with adult t-cell leukemia-lymphoma (atll) and transmission of htlv-i from b cells to normal t cells. | we analysed the dna of different tissues of a patient (hs) with adult t-cell leukemia/lymphoma virus (htlv-i). we detected viral sequences in fresh specimens from spleen, thymus, liver, skin and peripheral blood neoplastic lymphocytes. the pattern of htlv-i integration is identical in the leukemic cells and in all other tissues analysed, but the signal intensity is strongest in the leukemic cells, indicating the source of htlv-i proviral sequences was the leukemic t-cells which had infiltrated t ... | 1985 | 3001435 |
| [clinical picture of infectious mononucleosis in childhood]. | infectious mononucleosis, described for the first time many years ago, is still nowadays a frequent disease in children. history, etiology, pathogenesis and immunologic response to infectious mononucleosis are described. the value and limitations of diagnostic tests are discussed. the differential diagnosis, prognosis and possible complications are outlined. data from the literature are compared with results of our own experience with 35 serologically proven infections in children of different a ... | 1985 | 3001511 |
| [persistent (chronic active) epstein-barr virus infection and arthritis in childhood]. | between 1981 und 1983 some 1300 patients with the primary diagnosis of juvenile chronic arthritis were admitted. in 9 of them a persistent ebv-infection was simultaneously evident. the course of disease was primarily systemic with the characteristic criteria: high septic intermittent fever, rheumatic exanthema, hepatosplenomegaly and lymphadenopathy. it is still unclear whether a replication of virus in the synovia or a precipiation of immune complexes is involved. a common cause for the persist ... | 1985 | 3001512 |
| [burkitt's lymphoma in 1985]. | burkitt lymphoma is a model for either clinical research and fundamental research. initially described in africa, it is the most frequent of all childhood lymphoma. burkitt lymphoma allowed in the laboratory to study the virus cancer relationship, the chromosome cancer relationship, the gene and cancer relationship and the oncogene and cancer relationship. this study is made considerably more easy because of the numerous cell lines which were established from malignant cells at different stages ... | 1985 | 3001632 |
| [burkitt's lymphoma-epstein-barr virus association in western algeria. clinical, cytological and serological aspects apropos of 34 pediatric cases]. | burkitt's lymphoma (bl) and naso-pharyngeal carcinoma (npc) are tumors generally believed to be associated with epstein-barr virus (ebv). algerian population could be considered at high risk for bl-ebv association. in a series of 34 bl patients observed by us between 1982 and 1984 characteristics were: 70% of children were under 5 years age, abdominal location in 90% of cases (n = 30), 13% only isolated, massive tumor burden (70% stage iii after murphy, 30% stage iv after murphy and duque-hammer ... | 1985 | 3001633 |
| immunopathology of aids (acquired immune deficiency syndrome) observations in 75 patients with pre-aids and aids. | sequential changes in the morphology of immunocompetent and other organs, in the accompanying immunocytological and immunovirological measurements are described in 75 patients with lymphadenopathy syndrome and with aids. the data presented demonstrate a stepwise development of the disease from a hyperimmunization syndrome to t-cell immune deficiency. excessive antigenic stimulation by a large number of infectious organisms or by transfusion of blood and blood products account for antigenic overl ... | 1985 | 3001678 |
| analysis of the transcript encoding the latent epstein-barr virus nuclear antigen i: a potentially polycistronic message generated by long-range splicing of several exons. | the epstein-barr virus nuclear antigen (ebna i) present in latently infected cells is encoded in a 2-kilobase exon contained in the bamhi k viral genomic fragment. this exon is, however, found within a 3.7-kilobase mrna transcript. the origin of the remaining 1.7 kilobases is unknown, although it is not derived from adjacent epstein-barr virus dna sequences. a 1.1-kilobase cdna clone generated by primer extension using an oligonucleotide complimentary to a sequence 245 base pairs 3' to the putat ... | 1985 | 3001694 |
| cellular transformation by the herpesviruses and antiviral drugs. | 1985 | 3001790 | |
| epstein-barr virus-transformed b cell lines present m. leprae antigens to t cells. | we have investigated whether or not epstein-barr virus-transformed lymphoblastoid b cell lines (ebv-blcl) are able to present mycobacterium leprae (m. leprae) to antigen-reactive t cell lines and clones. such ebv-blcl would provide us with a homogeneous and unlimited source of antigen-presenting cells. antigen-triggered proliferation of t cells has been studied with co-cultures either with autologous or allogeneic irradiated ebv-blcl. our results show that ebv-blcl are able to present m. leprae ... | 1985 | 3001928 |
| a putative transforming gene of jijoye virus differs from that of epstein-barr virus prototypes. | the p3hr-1 strain of epstein-barr virus (ebv), a nontransforming clonal derivative of jijoye (ebv), is characterized by a deletion of 6.6 kb involving part of the bamhi-w repeats and the adjacent region including the noti repeats. in the transforming parental jijoye virus this region differs from the corresponding regions in b95-8 or m-aba virus. the hindiii-b fragments which carry this region from both jijoye and prototype m-aba (ebv) viruses have been cloned and subclones have been constructed ... | 1985 | 3002016 |
| review of the clinical manifestations, laboratory findings, and complications of infectious mononucleosis. | 1985 | 3002047 | |
| [the infantile acrodermatitis syndrome and epstein-barr virus infection]. | six cases of apvs, associated with epstein-barr-virus-infection are reported, the clinical picture, however, is not diagnosed as it is in acrodermatitis papulosa infantum (api). for this reason, we suggest to look for the signs of a primary viral infection in all cases of acro-localized papulo-vesicular skin manifestations. in contrast of apvs, api is a primary hepatitis b virus infection. | 1985 | 3002057 |
| effect of (e)-5-(2-bromovinyl)-2'-deoxyuridine on replication of epstein-barr virus in human lymphoblastoid cell lines. | the effect of (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu) on replication of epstein-barr virus (ebv) was investigated and compared with acyclovir (acv). both drugs inhibited ebv replication very rapidly. however, the inhibitory effect of acv was readily reversed after removal of the drug, in contrast to the more prolonged effect exerted by bvdu, which persisted for more than 21 days. the 50% inhibitory doses of bvdu for virus replication (ed50) and lymphoblastoid cell growth (id50) were 0.06 mic ... | 1985 | 3002251 |
| treatment and prevention of virus infections in immunosuppressed patients. | 1985 | 3002259 | |
| human antigen-specific t hybridomas. | production of antigen-specific hybridomas has been achieved by fusion of hgprt-negative mutant of jurkat and antigen-specific th cell lines. selection of clones for specificity was best accomplished by the binding of radiolabeled hybrid clones to antigen-pulsed monolayers. functional lymphokines such as il-2 and bcgf were induced by antigenic stimulation in the presence of ac. ac function of ebv-transformed autologous b cells was as efficient on a per cell basis as autologous monocytes. loss of ... | 1985 | 3002319 |
| specific b lymphocytes efficiently pick up, process and present antigen to t cells. | to study the nature of the antigen bridge between specific b and t lymphocytes we have isolated from the same donor clones of human t cells and ebv-transformed b cells specific for tetanus toxoid (tt). we found that tt-specific b cells are extremely efficient in presenting tt (but not an unrelated antigen) to t cells, as they can trigger t cell proliferation in the presence of only 10(-12) m tt, a concentration that is approximately 10(4) times lower than that required for presentation by conven ... | 1985 | 3002320 |
| the n-terminal portion of an erythrotropin-like peptide from fetal bovine serum has sequence homology with the ldl-receptor and two proteins of the epstein-barr virus. | an erythrotropin-like peptide (elp) and bovine serum erythrotropin (et) coeluted after more than four chromatographic steps. elp was separated from et on gel permeation high pressure liquid chromatography due to its large molecular weight. the effect of elp on the stimulation of [3h] thymidine incorporation in cell cultures of fetal bovine liver was much lower than the effect of et. the n-terminal amino acid sequence of elp indicated that it has a group of amino acids identical to portions of th ... | 1985 | 3002338 |
| structural difference of the insulin receptors from circulating monocytes and erythrocytes. | we compared insulin receptors obtained from cells widely used in human studies, the circulating monocytes and erythrocytes. biochemically, these receptors possess both binding (alpha-subunit) and tyrosine kinase (beta-subunit) activities similar to insulin receptors from other sources. subtle differences in molecular weight, however, were detected between the alpha-subunits of these two cell types when analyzed by nadodso4-page. crosslinked [125i]insulin-labeled alpha-subunit of the monocyte ins ... | 1985 | 3002369 |
| chromosomal localization of the epstein-barr virus (ebv) genome in bloom's syndrome b-lymphoblastoid cell lines transformed with ebv. | the localization of the epstein-barr virus (ebv) genome in chromosomes of human b-lymphoblastoid cell lines (lcls) transformed with ebv, and the effect of ebv dna on the level of sister chromatid exchange (sce) in bloom's syndrome (bs) b-lcls, were examined with chromosomal in situ hybridization techniques using a 3h-ebv dna probe. ebv dna was detected in chromosomes 1-5 and 13-15 at specific g band regions in bs as well as in normal b-lcls, regardless of sce. several chromosomal sites (1p31, 1q ... | 1985 | 3002731 |
| validity of lymphoid cell line for enzymatic studies of gm2-gangliosidosis variant 0 (sandhoff disease). | a lymphoid cell line established by epstein-barr virus (ebv)-transformation of peripheral blood b-lymphocytes from a patient with sandhoff disease showed a severe deficiency of beta-n-acetylhexosaminidase activity (residual activity around 10% of that in lymphoid cell lines from normals or other lipidotic patients). this residual beta-n-acetylhexosaminidase was completely heat-labile in contrast to that of normals. the molecular forms of residual beta-n-acetylhexosaminidase from sandhoff lymphoi ... | 1985 | 3002784 |
| glucocorticoid receptors in epstein-barr virus-transformed human lymphocytes. | glucocorticoid receptors were studied in cultured human lymphocytes from normal donors after transformation with the epstein-barr virus (ebv) and compared to those of circulating human mononuclear leukocytes. both whole cell and cytosol fractions were examined for [3h]-dexamethasone binding. the concentration and absolute number of glucocorticoid binding sites were increased five-fold in the transformed cells when compared to the non-transformed human mononuclear leukocytes. however, the affinit ... | 1985 | 3002935 |
| stress, loneliness, and changes in herpesvirus latency. | this study used a prospective design to examine the influence of examination stress and loneliness on herpesvirus latency as measured by changes in antibody levels to three herpesviruses, epstein-barr virus (ebv), herpes simplex type i (hsv-1), and cytomegalovirus (cmv). three blood samples were obtained from 49 first-year medical students, with the first sample drawn 1 month before final examinations, the second on the first day of final examinations, and the third during the first week after t ... | 1985 | 3003360 |
| [immunoblastic lymphadenopathy complicated by epstein-barr virus-positive primary brain lymphoma]. | 1985 | 3003420 | |
| [nasopharyngeal carcinoma with positive epstein-barr virus serology in a hodgkin's disease patient. viral reactivation?]. | 1985 | 3003735 | |
| [the advantages of using monoclonal antibodies in the transfusion service]. | 1985 | 3003879 | |
| xenohybridization of epstein-barr virus-transformed cells for the production of human monoclonal antibodies. | transformation of human b lymphocytes, obtained from hyperimmune donors with epstein-barr virus, yields polyclonal cell populations in which a minority of cells produce igg antibodies of predetermined specificity, whereas the majority of cells produce 'non-specific' immunoglobulin (mainly of the igm class). such lymphoblastoid cell lines can be easily propagated in high-density cultures. because cloning at 1 cell per well is not possible, stabilization of lymphoblastoid cell lines by limiting di ... | 1985 | 3003887 |
| [detection of epstein-barr viral antibodies in patients with acute viral hepatitis]. | serum samples obtained from 44 patients with virus a hepatitis, 23 patients with virus b hepatitis, 65 patients with virus non a, non b hepatitis and 100 healthy adults were studied for the presence of epstein-barr (eb) virus in the indirect immunofluorescence test. in this work lymphoblastoid cell lines ph3-j-1 and cn37 were used. among patients with different forms of hepatitis, the statistically significant elevation of the titers of antibodies to eb virus was detected only in the group of pa ... | 1985 | 3004073 |
| epstein-barr virus-positive primary brain lymphoma complicating immunoblastic lymphadenopathy. | a 54-year-old man developed primary brain lymphoma after immunosuppressive treatment for immunoblastic lymphadenopathy. the lymphoma cells were positive for the epstein-barr virus genome and had the surface phenotype of early b cells and a normal karyotype. | 1985 | 3004097 |
| burkitt's lymphoma. distinction of subgroups by morphometric analysis of the characteristics of 55 cell lines. | in an attempt to clarify the controversy about the distinction between burkitt's and non-burkitt's small noncleaved lymphomas, 55 cell lines derived from 48 burkitt's lymphoma patients were characterized by morphometry on plastic-embedded sections. the results of the measurements permitted the identification of five main cytologic types, with regard to nuclear size, nuclear area dispersion and irregularity of nuclear profiles. the presence of the epstein-barr virus (ebv) and the geographic origi ... | 1985 | 3004531 |
| a highly modular cloning vector for the analysis of eukaryotic genes and gene regulatory elements. | we have developed a highly modular vector, pdsp1, which contains two independent mammalian transcription cassettes. each cassette contains sv40 early gene regulatory elements controlling the expression of an easily assayable, selectable escherichia coli marker gene, either galk or xgprt. the regulatory elements of the galk cassette are bounded by multiple unique and nearly unique restriction sites allowing for the easy removal and replacement of either the regulatory sequences or of the galk gen ... | 1985 | 3004852 |
| recombinant vaccinia virus induces neutralising antibodies in rabbits against epstein-barr virus membrane antigen gp340. | the epstein-barr virus membrane antigen gene gp340 was isolated, inserted into several strains of vaccinia virus and expressed under the control of a vaccinia virus promoter. the ebv-derived protein which was produced by the recombinant vaccinia viruses was heavily glycosylated, readily labelled with threonine, could be detected at the surface of infected cells and had a mol. wt. of approximately 340 kd, all of which are properties of the authentic gp340. polyclonal rabbit antisera against gp340 ... | 1985 | 3004944 |
| human t lymphocyte clones specific for malaria (plasmodium falciparum) antigens. | peripheral blood mononuclear cells (pbm) from a patient who had lived in a malarial-endemic area were cultured in the presence of malarial antigens (a lysate of plasmodium-infected erythrocytes). responding cells were grown in il-2-containing medium and then cloned, and subsequently subcloned, in the presence of phytohemagglutinin and allogeneic irradiated pbm. ten clones were specific for malarial antigens. they proliferated in response to p. falciparum extract, but not to a lysate of uninfecte ... | 1985 | 3004971 |
| prevalence of anti-oncogenic virus antibodies in patients with malignancies, hemophilia and systemic lupus erythematosus. | 1985 | 3005471 | |
| acyclovir treatment in primary epstein-barr virus infection. a double-blind placebo-controlled study. | acyclovir (acv), which effectively inhibits in vitro epstein-barr virus (ebv) production, was tested in 31 patients with clinical and laboratory diagnosis of infectious mononucleosis (im) in a double-blind trial. patients with symptoms not exceeding 7 days were randomised to intravenous acv 10 mg/kg or placebo (plo) treatment every 8 h for 7 days. clinical, virological and immunological parameters were followed in each patient before, during and after treatment. there were no significant differe ... | 1985 | 3006226 |
| herpesvirus infections in the immunocompromised patient. | the herpes group viral infections in the t cell immunocompromised host may be seen as a disturbance of a delicate balance between the host and well adapted commensals. the clinical aspect of this disturbance is highly variable but may cause the destruction of the host due to widespread organ damage or prolonged debilitating illnesses. an important aspect is the immunosuppressive effect of the infections themselves leading to a number of superinfections, often with unusual pathogens, difficult to ... | 1985 | 3006227 |
| present and future of acyclovir. | acyclovir is now established as an effective and well tolerated therapeutic agent for the management of at least the more common infections of the herpes virus group. evaluation of the drug nevertheless continues, primarily to verify its value in those infections caused by cytomegalovirus (cmv) and epstein-barr virus (ebv). furthermore with the development of analogues of acyclovir with better absorption profiles or enhanced anti-viral activity the future for this area of anti-viral therapy look ... | 1985 | 3006231 |
| herpesvirus infection in man. | herpesviruses which affect man are herpes simplex virus type 1 and type 2, varicella-zoster virus, cytomegalovirus and epstein-barr virus. the review deals with the more common clinical manifestations of human herpesvirus infections, which occur ubiquitously in all populations throughout the world. primary infections most commonly occur in childhood. it is a characteristic feature of herpesvirus that they generally remain in a latent form after clearance of the primary infection. the overall maj ... | 1985 | 3006233 |
| biochemical and ultrastructural findings in a lymphoid cell line from niemann-pick disease type a. | a lymphoid cell line (lcl) established by epstein-barr virus (ebv)-transformation of blood b-lymphocytes from a patient affected with niemann-pick disease (npd) type a exhibited a severe deficiency of sphingomyelinase activity (less than 10% residual activity). ultrastructural investigation showed in lcl from npd type a, the presence of numerous osmiophilic, electron-dense inclusions with myelin-like figures characteristic of the accumulation of sphingomyelin (and other amphiphilic lipids) simil ... | 1985 | 3006848 |
| life span and 'immortalization' of mammalian cells. | 1985 | 3007349 | |
| the immunopathogenesis of rheumatoid arthritis. | rheumatoid arthritis (ra) may represent a t cell dependent immune response to a restricted antigen(s) within the joint, with inflammatory pathways reflecting secondary recruitment. the nature of the inciting antigen is unknown--the stimulus could be an infectious agent or a host constituent. in patients with ra, the centrality of antiself reactivities and immunogenetic influences is apparent. further clarification of these mechanisms would result in a potential for antigen-specific immunosuppres ... | 1985 | 3007756 |
| [late radiation effects of low doses from occupational exposure. epstein-barr virus-related antibody titers in radiological technologists]. | 1985 | 3007814 | |
| epstein-barr virus-related antibody pattern in uveitis. | epstein-barr virus-related antibody titers were determined in 30 patients with anterior uveitis, 45 with pan-uveitis, including 19 with behçet's disease, 11 with vogt-koyanagi-harada's disease and 15 with unclassified pan-uveitis, and 144 age-matched healthy controls. the frequencies of anti-vca (viro-capsid antigen) and anti-ea (early antigen) in uveitis patients and in controls were similar, but the frequency of sera with elevated titers (greater than or equal to 1:160) and the geometric mean ... | 1985 | 3007826 |
| characterization of stable epstein-barr (eb) virus transformed cell lines and mouse-human hybridomas producing a large quantity of anti-tetanus toxoid (tt) monoclonal antibody. | antigen-specific human monoclonal antibodies (hmoab) were investigated with particular emphasis on the stability and scale-up of production. peripheral blood lymphocytes from a healthy donor previously vaccinated with tetanus toxoid (tt) were resensitized in vitro with the antigen in the presence of pokeweed mitogen (pwm). three days after the stimulation, lymphocytes were infected with epstein-barr (eb) virus. among eb virus transformants, several lymphoblastoid cell clones producing anti-tt hm ... | 1985 | 3008706 |
| [viral aspects of nasopharyngeal cancer]. | 1985 | 3008960 |