Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| optimization of the timing of induction for the assessment of nitric oxide production in leishmania major infected macrophage cells. | the present study was conducted to investigate the optimized timing for macrophages induction and nitric oxide (no) production against invading leishmania parasite. | 2017 | 28127337 |
| leishmania major: genetic profiles of the parasites isolated from chabahar, southeastern iran by ppip-pcr. | leishmaniasis is important vector-borne parasitic disease worldwide, caused by the genus leishmania. the objective of the current study was to identify genetic polymorphism in l. major, one of the species causing cutaneous leishmaniasis (cl), isolated from southeastern iran, using permissively primed intergenic polymorphic-polymerase chain reaction (ppip-pcr) method. | 2017 | 28127333 |
| expression analysis of multiple genes may involve in antimony resistance among leishmania major clinical isolates from fars province, central iran. | treatment of cutaneous leishmaniasis (cl) is being faced with serious difficulties in fars province, due to emerging of resistance against meglumine antimonite (glucantime®). in this context, determining some biomarkers for drug sensitivity monitoring seems to be highly essential. different studies have been carried out to decipher the genes might be involved in antimony resistant phenotype in leishmania spp. here, we selected three genes: aqp (as drug transporter), tdr-1-1(as drug activator), a ... | 2017 | 28096850 |
| continual renewal and replication of persistent leishmania major parasites in concomitantly immune hosts. | in most natural infections or after recovery, small numbers of leishmania parasites remain indefinitely in the host. persistent parasites play a vital role in protective immunity against disease pathology upon reinfection through the process of concomitant immunity, as well as in transmission and reactivation, yet are poorly understood. a key question is whether persistent parasites undergo replication, and we devised several approaches to probe the small numbers in persistent infections. we fin ... | 2017 | 28096392 |
| polymerase chain reaction detection and inducible nitric-oxide synthase expression of leishmania major in mice inoculated by two different routes. | leishmania major needs a sensitive and specific method for proper diagnosis. this study aims to study the course and histopathology of l. major in certain tissues of experimentally infected balb/c mice after subcutaneous (sc) and intraperitoneal (ip) inoculation. | 2017 | 26998433 |
| imported cutaneous leishmaniasis caused by leishmania major in a chinese laborer who worked in saudi arabia. | we report an imported case of cutaneous leishmaniasis in a 37-year-old man from saudi arabia caused by leishmania major. he presented with non-healing nodulo-ulcerative lesions with a "volcanic crater" on the lower limbs. it was clearly cutaneous leishmaniasis - a rare disease in china - as reflected by the patient's clinical history, the lesions' morphology, histopathological examination, culture and pcr analysis of the lesions. the patient was completely cured after two cycles of sodium stibog ... | 2017 | 27438208 |
| molecular detection of leishmania parasites and host blood meal identification in wild sand flies from a new endemic rural region, south of iran. | zoonotic cutaneous leishmaniosis (zcl) remains the most crucial vector-borne public health disease particularly in endemic rural parts of iran. the main aim of this study is to identify wild sand flies (diptera: psychodidae), determine their infection rate, and differentiate their host blood meal sources using the polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) technique. sand fly populations were caught with sticky paper traps from 10 different villages in the coun ... | 2017 | 27854189 |
| comparative genomics of tunisian leishmania major isolates causing human cutaneous leishmaniasis with contrasting clinical severity. | zoonotic cutaneous leishmaniasis caused by leishmania (l.) major parasites affects urban and suburban areas in the center and south of tunisia where the disease is endemo-epidemic. several cases were reported in human patients for which infection due to l. major induced lesions with a broad range of severity. however, very little is known about the mechanisms underlying this diversity. our hypothesis is that parasite genomic variability could, in addition to the host immunological background, co ... | 2017 | 27818279 |
| genomic cartography and proposal of nomenclature for the repeated, interspersed elements of the leishmania major sider2 family and identification of sider2-containing transcripts. | the genomes of most eukaryotic organisms contain a large number of transposable elements that are able to move from one genomic site to another either by transferring of dna mobile elements (transposons) or transpose via reverse transcription of an rna intermediate (retroposons). an exception to this rule is found in protists of the subgenus leishmania, in which active retroposons degenerated after a flourishing era, leaving only retroposon remains; these have been classified into two families: ... | 2017 | 28034676 |
| in vitro antileishmanial activity of novel azoles (3-imidazolylflavanones) against promastigote and amastigote stages of leishmania major. | leishmaniasis is a protozoan infectious disease widely distributed all around the world. first line drugs including antimoniales are insufficient due to resistance in endemic areas and high toxicity. azole antifungals like ketoconazole (kcz) are also used as antileishmanial agents for several decades. in the present study, we evaluated in vitro antileishmanial effects of new azole antifungals namely 3-imidazolylflavanones (ifs) and their oximes (ifos) against leishmania major (l. major) parasite ... | 2017 | 28017860 |
| a novel protein coding potential of long intergenic non-coding rnas (lincrnas) in the kinetoplastid protozoan parasite leishmania major. | cutaneous leishmaniasis (cl) is caused by a kinetoplastid protozoan parasite leishmania major, as a skin ulcer at the site of the sandfly bite. cl is curable and in most cases ulcers heal spontaneously within three to six months leaving a scar and disfiguration. complete genome of l. major was reported in 2005 at the very initial phase of kinetoplastid parasite genome sequencing project. presently, l. major genome is most studied and comprehensively annotated genome and therefore, it is being us ... | 2017 | 27988178 |
| parasites dampen dendritic cell activation to ensure their survival. | dendritic cells (dcs) are critical for induction of protective immunity against leishmania major. however, dc activation occurs only several weeks after parasite transmission. parasites synthesize a macrophage-inducible c-type lectin (mincle) ligand. engagement of mincle by the parasite ligand dampens dc activation, thus delaying induction of interferon-γ-producing t cells responsible for parasite eradication. | 2017 | 27988097 |
| amiodarone triggers induction of apoptosis in cutaneous leishmaniasis agents. | leishmaniasis is a parasitic disease that is an important problem of public health worldwide. the aim of this study was to assess the cytotoxic effects of amiodarone (amd) on leishmania tropica (mhom/af/88/kk27) and l. major (mrho/ir/75/er) promastigotes and to observe the programmed cell death features. the colorimetric mtt assay was used to find l. tropica and l. major viability and the obtained results were expressed as 50% inhibitory concentration (ic50). annexin-v fluos staining was perform ... | 2017 | 27553974 |
| leishmania hasp and sherp genes are required for in vivo differentiation, parasite transmission and virulence attenuation in the host. | differentiation of extracellular leishmania promastigotes within their sand fly vector, termed metacyclogenesis, is considered to be essential for parasites to regain mammalian host infectivity. metacyclogenesis is accompanied by changes in the local parasite environment, including secretion of complex glycoconjugates within the promastigote secretory gel and colonization and degradation of the sand fly stomodeal valve. deletion of the stage-regulated hasp and sherp genes on chromosome 23 of lei ... | 2017 | 28095465 |
| in silico prediction of leishmania major-specific cd8(+) epitopes. | 2017 | 28094883 | |
| cytotoxic, anti-inflammatory, and leishmanicidal activities of diterpenes isolated from the roots of caesalpinia pulcherrima. | a phytochemical investigation on the chloroform extract of caesalpinia pulcherrima roots led to the isolation of ten known furanocassane diterpenoids, vouacapen-5α-ol (1), 8,9,11,14-didehydrovouacapen-5α-ol (2), 6β-cinnamoyl-7β-hydroxyvouacapen-5α-ol (3), pulcherrin a (4), pulcherrin b (5), pulcherrin j (6), pulcherrimin a (7), pulcherrimin b (8), pulcherrimin c (9), and pulcherrimin e (10). chemical transformation of 3 and 7 gave compounds 6β-hydroxyisovouacapenol c (11), 6β-cinnamoyl-7β-acetox ... | 2017 | 27340793 |
| utility of western blot analysis for the diagnosis of cutaneous leishmaniasis. | cutaneous leishmaniasis (cl) is a parasitic disease with a relatively wide distribution in different areas of the world, including iran. the parasite is mainly diagnosed microscopically, but serological approaches might be useful for diagnosis as well. this study aimed to assess the efficacy of an immunoblotting system for serodiagnosis of cutaneous leishmaniasis in iran. | 2017 | 26811727 |
| comparison of parasite burden using real-time polymerase chain reaction assay and limiting dilution assay in leishmania major infected mouse. | limiting dilution assay is considered as the gold standard method for quantifying the number of parasites in the animal model of leishmania infection. nowadays, real-time pcr is being increasingly applied to quantify infectious agents. in the present study, a real-time pcr assay was developed to estimate parasite burdens in lymph nodes of leishmania major infected balb/c mice. enumeration of parasites was also performed by limiting dilution assay and compared with the results of real-time pcr ba ... | 2017 | 26811723 |
| interplay between a cytosolic and a cell surface carbonic anhydrase in ph homeostasis and acid tolerance of leishmania. | leishmania parasites have evolved to endure the acidic phagolysosomal environment within host macrophages. how leishmania cells maintain near-neutral intracellular ph and proliferate in such a proton-rich mileu remains poorly understood. we report here that, in order to thrive in acidic conditions, leishmania major relies on a cytosolic and a cell surface carbonic anhydrase, lmca1 and lmca2, respectively. upon exposure to acidic medium, the intracellular ph of the lmca1(+/-), lmca2(+/-) and lmca ... | 2017 | 28062849 |
| antileishmanial activities of greek juniper (juniperus excelsa m.bieb.) against leishmania major promastigotes. | petroleum ether, chloroform, ethyl acetate, and n-butanol fractions of greek juniper (juniperus excelsa m.bieb. from the family cupressaceae) were evaluated for antileishmanial activities against leishmania major promastigotes compared to meglumine antimoniate (glucantime). in vitro toxicity assay was performed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide and microplate elisa reader. extracts were prepared in ethanol/dimethyl sulfoxide (80/20) at 10 to 0.62 mg/ml. the stan ... | 2017 | 26747836 |
| double-blind randomized efficacy field trial of alum precipitated autoclaved leishmania major (alum-alm) vaccine mixed with bcg plus imiquimod vs. placebo control group. | canine visceral leishmaniasis (cvl) is not only an emerging veterinary concern but also a public health threat in endemic areas. the aim of this study was to assess the efficacy, immunogenicity and safety of two doses of aluminum hydroxide (alum) precipitated leishmania major (alum-alm) mixed with bcg plus imiquimod against cvl. | 2017 | 26622290 |