Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| growth and sporulation of bacillus cereus atcc 14579 under defined conditions: temporal expression of genes for key sigma factors. | an airlift fermentor system allowing precise regulation of ph and aeration combined with a chemically defined medium was used to study growth and sporulation of bacillus cereus atcc 14579. sporulation was complete and synchronous. expression of siga, sigb, sigf, and sigg was monitored with real-time reverse transcription-pcr, and the pattern qualitatively resembled that of bacillus subtilis. this method allows reproducible production of stable spores, while the synchronous growth and defined con ... | 2004 | 15066852 |
| shelf life and safety concerns of bakery products--a review. | bakery products are an important part of a balanced diet and, today, a wide variety of such products can be found on supermarket shelves. this includes unsweetened goods (bread, rolls, buns, crumpets, muffins and bagels), sweet goods (pancakes, doughnuts, waffles and cookies) and filled goods (fruit and meat pies, sausage rolls, pastries, sandwiches, cream cakes, pizza and quiche). however, bakery products, like many processed foods, are subject to physical, chemical and microbiological spoilage ... | 2004 | 15077880 |
| cytotoxic bacillus spp. belonging to the b. cereus and b. subtilis groups in norwegian surface waters. | to investigate the presence and numbers of bacillus spp. spores in surface waters and examine isolates belonging to the b. cereus and b. subtilis groups for cytotoxicity, and to discuss the presence of cytotoxic bacillus spp. in surface water as hazard identification in a risk assessment approach in the food industry. | 2004 | 15078515 |
| influence of surface chemistry on the hygienic status of industrial stainless steel. | coupons of fourteen different stainless steels were investigated in terms of surface chemistry and ease of cleaning. steel surfaces were exposed to bacillus cereus spores in static saline solution for 2 h. surfaces were rinsed and then covered with whole milk and allowed to dry. surfaces were then cleaned in an experimental flow system that mimics an industrial application. after cleaning, remaining spores were released by sonication, spores cultured and colony forming units determined. surfaces ... | 2004 | 15079890 |
| identification of pathogenic microbial cells and spores by electrochemical detection on a biochip. | background: bacillus cereus constitutes a significant cause of acute food poisoning in humans. despite the recent development of different detection methods, new effective control measures and better diagnostic tools are required for quick and reliable detection of pathogenic micro-organisms. thus, the objective of this study was to determine a simple method for rapid identification of enterotoxic bacillus strains. here, a special attention is given to an electrochemical biosensor since it meets ... | 2004 | 15090067 |
| isolation of a minireplicon of the virulence plasmid pxo2 of bacillus anthracis and characterization of the plasmid-encoded reps replication protein. | a minireplicon of plasmid pxo2 of bacillus anthracis was isolated by molecular cloning in escherichia coli and shown to replicate in b. anthracis, bacillus cereus, and bacillus subtilis. the pxo2 replicon included (i) an open reading frame encoding the putative reps replication initiation protein and (ii) the putative origin of replication. the reps protein was expressed as a fusion with the maltose binding protein (mbp) at its amino-terminal end and purified by affinity chromatography. electrop ... | 2004 | 15090513 |
| composition and bioactivity of the leaf essential oil of heteropyxis dehniae from zimbabwe. | the leaf oil of heteropyxis dehniae suess. (heteropyxidaceae) was obtained by hydrodistillation and analyzed by gc/ms. the most abundant essential oil components are linalool (58.3%), 4-terpineol (9.8%), alpha-terpineol (3.6%), and caryophyllene oxide (3.1%). the antimicrobial activity against bacillus cereus, staphylococcus aureus, pseudomonas aeruginosa, escherichia coli, candida albicans, and aspergillus niger, and the in vitro cytotoxicity of the oil on pc-3, mda-mb-231, hs 578t, mcf7, sk-me ... | 2004 | 15099856 |
| new chromanone acids with antibacterial activity from calophyllum brasiliense. | six novel chromanone acids (1-6) were isolated from the bark of calophyllum brasiliense cambess. their structures were elucidated on the basis of 1d and 2d nmr experiments, as well as mass spectrometry. all compounds showed moderate to strong antibacterial activity against bacillus cereus and staphylococcus epidermidis, with 1 and 2 being most active. none of the compounds were cytotoxic against kb, jurkat t, and myosarcoma cancer cells up to 20 microg/ml. | 2004 | 15104480 |
| rugulotrosins a and b: two new antibacterial metabolites from an australian isolate of a penicillium sp. | two new antibacterial agents, rugulotrosin a (1) and b (2), were obtained from cultures of a penicillium sp. isolated from soil samples acquired near sussex inlet, new south wales, australia. rugulotrosin a (1) is a chiral symmetric dimer, and its relative stereostructure was determined by spectroscopic and x-ray crystallographic analysis. rugulotrosin b (2) is a chiral asymmetric dimer isomeric with 1. its structure was determined by spectroscopic analysis with comparison to the co-metabolite 1 ... | 2004 | 15104517 |
| weathering of phlogopite by bacillus cereus and acidithiobacillus ferrooxidans. | the purpose of this study was to assess the weathering of finely ground phlogopite, a trioctahedral mica, by placing it in contact with heterotrophic (bacillus cereus) and acidophilic (acidithiobacillus ferrooxidans) cultures. x-ray diffraction analyses of the phlogopite sample before and after 24 weeks of contact in b. cereus cultures revealed a decrease in the characteristic peak intensities of phlogopite, indicating destruction of individual structural planes of the mica. no new solid phase p ... | 2004 | 15105888 |
| investigation of metal ion binding in phosphonoacetaldehyde hydrolase identifies sequence markers for metal-activated enzymes of the had enzyme superfamily. | the 2-haloalkanoic acid dehalogenase (had) family, which contains both carbon and phosphoryl transferases, is one of the largest known enzyme superfamilies. had members conserve an alpha,beta-core domain that frames the four-loop active-site platform. each loop contributes one or more catalytic groups, which function in mediating the core chemistry (i.e., group transfer). in this paper, we provide evidence that the number of carboxylate residues on loop 4 and their positions (stations) on the lo ... | 2004 | 15109258 |
| use of novel pcr primers specific to the genes of staphylococcal enterotoxin g, h, i for the survey of staphylococcus aureus strains isolated from food-poisoning cases and food samples in taiwan. | data regarding the incidence of the newly found enterotoxigenic staphylococcus aureus strains in food poisoning cases and in food samples were to date not available in taiwan. in this study, pcr primers specific for the detection of seg, h and i genes, i.e., seg, seh and sei, were used for the assay of 55 human isolates of s. aureus negative to the classical enterotoxins (sea-->see) detection. these isolates were from the fecal specimens of the patients suffering from food poisoning outbreaks. o ... | 2004 | 15109796 |
| inactivation of bacillus cereus spores in milk by mild pressure and heat treatments. | the objective of this work was to study the germination and subsequent inactivation of bacillus cereus spores in milk by mild hydrostatic pressure treatment. in an introductory experiment with strain lmg6910 treated at 40 degrees c for 30 min at 0, 100, 300 and 600 mpa, germination levels were 1.5 to 3 logs higher in milk than in 100 mm potassium phosphate buffer (ph 6.7). the effects of pressure and germination-inducing components present in the milk on spore germination were synergistic. more ... | 2004 | 15109800 |
| the antibacterial innate immune response by the mosquito aedes aegypti is mediated by hemocytes and independent of gram type and pathogenicity. | previous mosquito studies showed that the hemocyte-mediated innate immune response against gram- escherichia coli is phagocytosis, but against gram+ micrococcus sp., is melanization. we examined the immune responses mounted by aedes aegypti towards gram- enterobacter cloacae, klebsiella pneumoniae, pseudomonas aeruginosa, and salmonella typhimurium, and gram+ bacillus cereus, enterococcus faecalis, staphylococcus aureus, and staphylococcus epidermidis. using light microscopy, electron microscopy ... | 2004 | 15109959 |
| cloning and expression of a putative transferrin cdna of the spruce budworm, choristoneura fumiferana. | a spruce budworm (choristoneura fumiferana) transferrin cdna (cftf) was isolated and cloned from a cdna library that was constructed using mrna from fifth to sixth instar larvae. cftf cdna encoded a predicted protein of 681 amino acids with a molecular mass of approximately 76 kda. cftf shared 72% and 74% identities at the amino acid level with transferrins of manduca sexta and bombyx mori, respectively. like other transferrins, cftf retains most of the n-terminal, iron-binding amino acid residu ... | 2004 | 15110871 |
| synthesis and biological evaluation of oxindoles and benzimidazolinones derivatives. | the synthesis of new oxindoles and benzimidazolinones derivatives bearing a sugar residue on the aromatic nitrogen is described. the presence of the glycoside moiety should enhance the solubility of these heterocyclic compounds and/or improve the interaction with the active site of the biological targets. the inhibitory activities of these new compounds toward five kinases were examined: kdr (vegfr-2), fgfr-1, pdgfr-beta, egfr and tie 2. furthermore, the antibacterial activities of the prepared ... | 2004 | 15110971 |
| two additional carbohydrate-binding sites of beta-amylase from bacillus cereus var. mycoides are involved in hydrolysis and raw starch-binding. | in the previous x-ray crystallographic study, it was found that beta-amylase from bacillus cereus var. mycoides has three carbohydrate-binding sites aside from the active site: two (site2 and site3) in domain b and one (site1) in domain c. to investigate the roles of these sites in the catalytic reaction and raw starch-binding, site1 and site2 were mutated. from analyses of the raw starch-binding of wild-type and mutant enzymes, it was found that site1 contributes to the binding affinity to raw- ... | 2004 | 15113834 |
| evaluation of the sanitization effectiveness of a denture-cleaning product on dentures contaminated with known microbial flora. an in vitro study. | to see if dentures contaminated with staphylococcus aureus, pseudomas aeruginosa, bacillus cereus, candida albicans, and herpes simplex virus 1 could be effectively decontaminated by using medical tabs for dentures. | 2004 | 15119677 |
| synergistic interaction in simultaneous exposure to streptomyces californicus and stachybotrys chartarum. | the microbial exposure associated with health complaints in moldy houses consists of a heterogeneous group of components, including both living and dead bacteria, fungi, and their metabolites and active compounds. however, little is known about the interactions between different microbes and their metabolites, although the cytotoxicity and inflammatory potential of certain individual microbes have been reported. in this study, we investigated the inflammatory responses of mouse raw264.7 macropha ... | 2004 | 15121507 |
| bacillus cereus central line infection in an immunocompetent child with hemophilia. | bacillus species are increasingly recognized as pathogens in immunocompromised patients. the authors report a case of bacillus cereus infection of a central line in an immunocompetent patient with hemophilia, which required line removal for complete cure. | 2004 | 15125613 |
| an extracytoplasmic-function sigma factor is involved in a pathway controlling beta-exotoxin i production in bacillus thuringiensis subsp. thuringiensis strain 407-1. | beta-exotoxin i is an insecticidal nucleotide analogue secreted by various bacillus thuringiensis strains. in this report, we describe the characterization and transcriptional analysis of a gene cluster, designated sigw-ecfx-ecfy, that is essential for beta-exotoxin i production in b. thuringiensis subsp. thuringiensis strain 407-1. in this strain, the disruption of the sigw cluster resulted in nontoxic culture supernatants. sigw encodes a protein of 177 residues that is 97 and 94% identical to ... | 2004 | 15126472 |
| growth promotion of red pepper plug seedlings and the production of gibberellins by bacillus cereus, bacillus macroides and bacillus pumilus. | the growth of red pepper plug seedlings was promoted by bacillus cereus mj-1, b. macroides cj-29, and b. pumilus cj-69 isolated from the rhizosphere. gibberellins (gas), a well-known plant growth-promoting hormone, were detected in the culture broth of their rhizobacteria. among the gas, the contents of ga1, ga3, ga4, and ga7, physiologically active gas, were comparatively higher than those of others, suggesting that the growth promoting effect was originated from the gas. this isthe first repor ... | 2004 | 15127789 |
| genome update: rrnas in sequenced microbial genomes. | 2004 | 15133066 | |
| strains of bacillus cereus vary in the phenotypic adaptation of their membrane lipid composition in response to low water activity, reduced temperature and growth in rice starch. | the phenotypic adaptation of membrane lipids in seven strains of the food-poisoning bacterium bacillus cereus, isolated from bangladeshi rice, is reported in relation to their ability to grow under conditions of low water activity (a(w)), reduced temperature and the presence of soluble rice starch. the strains have different membrane phospholipid head-group and fatty acyl compositions, and they display individual differences in their responses to both low a(w) and reduced temperature. the extent ... | 2004 | 15133101 |
| strength in diversity. | 2004 | 15134078 | |
| attenuation regulation of amino acid biosynthetic operons in proteobacteria: comparative genomics analysis. | candidate attenuators were identified that regulate operons responsible for biosynthesis of branched amino acids, histidine, threonine, tryptophan, and phenylalanine in gamma- and alpha-proteobacteria, and in some cases in low-gc gram-positive bacteria, thermotogales and bacteroidetes/chlorobi. this allowed us not only to describe the evolutionary dynamics of regulation by attenuation of transcription, but also to annotate a number of hypothetical genes. in particular, orthologs of ygea of esche ... | 2004 | 15135544 |
| detection of bacterial dna in painful degenerated spinal discs in patients without signs of clinical infection. | a local inflammatory and potentially painful response, of which the ultimate cause is unknown, has been described in nervous tissues in contact with degenerated disc material in patients with low back and leg pain. with the rationale that a possible cause of such inflammation could be bacterial infection, we utilized pcr (polymerase chain reaction) amplification of the 16s rrna (ribosomal rna) gene followed by gene sequencing, to investigate whether bacterial dna might be detected in the degener ... | 2004 | 15138861 |
| [techniques of diseases, insect pests and weeds control and their efficacy in bio-rational rice production]. | studies on the efficacy of bio-rational pesticides and agricultural methods against the chief diseases, insect pests and weeds of rice showed that the efficacy of the mixtures of jingangmycin and bacillus-cereus, and jingangmycin and polyoxin against rice sheath blight were 75.16%-94.27% after sprayed once at the tiller and boot end stages of rice, respectively, and better than that of chemical fungicide triadimefon. the efficacy of kasugamycin and blasticidin was 50.54%-72.67% on rice leaf blas ... | 2004 | 15139200 |
| structural determinants of substrate binding to bacillus cereus metallo-beta-lactamase. | binding and hydrolysis of the beta-lactams cefotaxime, cephapirin, imipenem, and benzylpenicillin by the metallo-beta-lactamase from bacillus cereus were studied by presteady state kinetic measurements. in all cases, the substrate was unmodified in the most populated reaction intermediate, and no chemically modified substrate species accumulated to a detectable amount. the cephalosporins tested showed similar formation rate constants for this intermediate, and they differed mostly in their decay ... | 2004 | 15140877 |
| venom from the pupal endoparasitoid, pimpla hypochondriaca, increases the susceptibility of larval lacanobia oleracea to the entomopathogens bacillus cereus and beauveria bassiana. | cellular immune responses in insects protect them against parasites and pathogens that enter their hemocoel. venom from the solitary pupal endoparasitoid, pimpla hypochondriaca, has previously been shown to suppress certain key, cell-mediated immune responses of lacanobia oleracea. experiments were performed to determine if l. oleracea larvae injected with p. hypochondriaca venom would be more susceptible to bacillus cereus, or beauveria bassiana, when these microorganisms were subsequently inje ... | 2004 | 15145247 |
| distinct mutations in plcr explain why some strains of the bacillus cereus group are nonhemolytic. | bacillus thuringiensis, bacillus cereus, and bacillus anthracis are closely related species belonging to the bacillus cereus group. b. thuringiensis and b. cereus generally produce extracellular proteins, including phospholipases and hemolysins. transcription of the genes encoding these factors is controlled by the pleiotropic regulator plcr. disruption of plcr in b. cereus and b. thuringiensis drastically reduces the hemolytic, lecithinase, and cytotoxic properties of these organisms. b. anthra ... | 2004 | 15150241 |
| variation of the spore population of a natural source strain of bacillus cereus in the presence of inosine. | the heat resistance of a wild strain of bacillus cereus spores isolated from liquid egg was characterized, and the effect of the nutritional germinant inosine on the spore population was then studied, considering different factors such as germination temperature, inosine concentration, and age of spore culture. the heat resistance clearly indicates that these spores can survive mild heat treatments such as those used for cooked refrigerated food of extended durability or liquid egg, posing safet ... | 2004 | 15151230 |
| modeling the prevalence of bacillus cereus spores during the production of a cooked chilled vegetable product. | in minimally processed vegetable foods, pathogenic spore-forming bacteria pose a significant hazard. as part of a quantitative risk assessment, we used bayesian belief methods to model the uncertainty and variability of the number of bacillus cereus spores that can be found in packets of a vegetable puree. the model combines specific information from the manufacturer, experimental data on inactivation of spores, and expert opinion concerning spore concentrations in the raw vegetables and ingredi ... | 2004 | 15151231 |
| sensitization of gram-negative and gram-positive bacteria to jenseniin g by sublethal injury. | jenseniin g, a bacteriocin produced by propionibacterium thoenii p126, is active against related propionibacteria and some lactic acid bacteria and is sporostatic to botulinal spores. the objective of this study was to evaluate the effects of sublethal stress on jenseniin g activity. bacillus cereus, enterococcus faecalis, escherichia coli, listeria monocytogenes, salmonella typhimurium, shigella flexneri, staphylococcus aureus, and yersinia enterocolitica were subjected to temperature, acid, an ... | 2004 | 15151241 |
| inactivation of pathogenic bacteria by cucumber volatiles (e,z)-2,6-nonadienal and (e)-2-nonenal. | the effects of (e,z)-2,6-nonadienal (nde) and (e)-2-nonenal (ne) on bacillus cereus, escherichia coli o157:h7, listeria monocytogenes, and salmonella typhimurium were investigated. a suspension of each organism of 6 to 9 log cfu/ml was incubated for 1 h at 37 degrees c in brain heart infusion solution that contained 0 to 500 or 1,000 ppm of nde or ne. depending on concentration, exposure to either nde or ne caused a reduction in cfu of each organism. treatment with 250 and 500 ppm nde completely ... | 2004 | 15151242 |
| characterization of bacillus cereus isolates from raw soybean sprouts. | raw soybean sprouts, which are used as ingredients in cook-chilled products, were analyzed to evaluate the incidence of mesophilic aerobic microorganisms, psychrotrophic microorganisms, anaerobic microorganisms, coliforms, and spore-forming microorganisms bacillus cereus, clostridium botulinum, and clostridium perfringens. mesophilic microorganisms on raw soybean sprouts were present in large populations, 5.5 x 10(6) to 1.4 x 10(8) cfu/g, and psychrotrophic microorganisms were found to be more n ... | 2004 | 15151246 |
| novel oligosaccharide side chains of the collagen-like region of bcla, the major glycoprotein of the bacillus anthracis exosporium. | spores of bacillus anthracis, the causative agent of anthrax, are enclosed by a prominent loose fitting layer called the exosporium. the exosporium consists of a basal layer and an external hairlike nap. the filaments of the nap are composed of a highly immunogenic glycoprotein called bcla, which has a long, central collagen-like region with multiple xxg repeats. most of the triplet repeats are ptg, and nearly all of the triplet repeats contain a threonine residue, providing multiple potential s ... | 2004 | 15152001 |
| disassembly of f-actin cytoskeleton after interaction of bacillus cereus with fully differentiated human intestinal caco-2 cells. | in the present study, the role of direct procaryote-eucaryote interactions in the virulence of bacillus cereus was investigated. as a model of human enterocytes, differentiated caco-2 cells were used. infection of fully differentiated caco-2 cells with b. cereus in the exponential phase of growth, in order to minimize the concentration of spores or sporulating microorganisms, shows that a strain-dependent cytopathic effect develops. interestingly, addition of 3-h-old cultures of some strains res ... | 2004 | 15155611 |
| identification of anthrax toxin genes in a bacillus cereus associated with an illness resembling inhalation anthrax. | bacillus anthracis is the etiologic agent of anthrax, an acute fatal disease among mammals. it was thought to differ from bacillus cereus, an opportunistic pathogen and cause of food poisoning, by the presence of plasmids pxo1 and pxo2, which encode the lethal toxin complex and the poly-gamma-d-glutamic acid capsule, respectively. this work describes a non-b. anthracis isolate that possesses the anthrax toxin genes and is capable of causing a severe inhalation anthrax-like illness. although init ... | 2004 | 15155910 |
| purification of bacteriocins produced by lactic acid bacteria. | bacteriocins are antibacterial substances of a proteinaceous nature that are produced by different bacterial species. lactic acid bacteria (lab) produce biologically active peptides or protein complexes that display a bactericidal mode of action almost exclusively toward gram-positive bacteria and particularly toward closely related species. generally they are active against food spoilage and foodborne pathogenic microorganisms including bacillus cereus, clostridium perfringens, staphylococcus a ... | 2004 | 15156043 |
| investigation of the effectiveness of ascopyrone p as a food preservative. | ascopyrone p (app), a novel antibacterial from fungi, was evaluated as a food preservative. efficacy was generally assessed by comparing the time taken for test strains to grow to 10(6) cfu/g in food +/- app. in chilled chicken soup, 2000 mg kg-1 app prevented bacillus cereus, listeria monocytogenes, pseudomonas fluorescens, salmonella and escherichia coli reaching this threshold for >60 days. good activity was also observed at 500-1000 mg kg-1 but not against l. monocytogenes. no activity was o ... | 2004 | 15163588 |
| cholesterol modulation of sphingomyelinase activity at physiological temperatures. | bacillus cereus sphingomyelinase activity was assayed on large unilamellar vesicles composed of sphingomyelin (sm)/cholesterol (ch) mixtures at varying proportions. natural (egg) sm was used with a gel-fluid transition temperature at ca. 40 degrees c. when the enzyme was assayed at 37 degrees c, the activity on pure sm was exceedingly low, but a small increase was observed as soon as some ch was added, and a large enhancement of activity occurred with ch proportions above 25 mol%. the data were ... | 2004 | 15172829 |
| inhibition of the growth of ascosphaera apis by bacillus and paenibacillus strains isolated from honey. | the fungus ascosphaera apis, the causative agent of chalkbrood disease in honeybee larvae, occurs throughout the world and is found in many beekeeping areas of argentina. the potential as biocontrol agents of 249 aerobic spore-forming bacterial antagonists isolated from honey samples was evaluated. each isolate was screened against a. apis by a central disk test assay. ten bacterial strains that showed the best antagonistic effect to a. apis were selected for further study and identified as baci ... | 2004 | 15174751 |
| cytoplasmic membrane polarization in gram-positive and gram-negative bacteria grown in the absence and presence of tetracycline. | the ability of numerous diverse compounds and ions to cross the bacterial cytoplasmic membrane by diffusion and active transport is highly dependent on cytoplasmic membrane fluidity, which can be measured using fluorescent probes to estimate membrane polarization values. however, membrane polarization data are lacking for most bacterial species. the cytoplasmic membrane polarization values for arthrobacter sp. atcc 21908, bacillus cereus nrc 3045, pseudomonas fluorescens r2f, pseudomonas putida ... | 2004 | 15182931 |
| isolation, characterization, and identification of bacterial contaminants in semifinal gelatin extracts. | bacterial contamination of gelatin is of great concern. indeed, this animal colloid has many industrial applications, mainly in food and pharmaceutical products. in a previous study (e. de clerck and p. de vos, syst. appl. microbiol. 25:611-618), contamination of a gelatin production process with a variety of gram-positive and gram-negative bacteria was demonstrated. in this study, bacterial contamination of semifinal gelatin extracts from several production plants was examined. since these extr ... | 2004 | 15184171 |
| survival of bacillus cereus spores and vegetative cells in acid media simulating human stomach. | to determine the fate of bacillus cereus spores or vegetative cells in simulated gastric medium. methods and | 2004 | 15186458 |
| insulator-based dielectrophoresis for the selective concentration and separation of live bacteria in water. | insulator-based dielectrophoresis (idep) was utilized to separate and concentrate selectively mixtures of two species of live bacteria simultaneously. four species of bacteria were studied: the gram-negative escherichia coli and the gram-positive bacillus subtilis, b. cereus, and b. megaterium. under an applied direct current (dc) electric field all the bacterial species exhibited negative dielectrophoretic behavior. the dielectrophoretic separations were carried out in a glass microchannel cont ... | 2004 | 15188259 |
| antimicrobial activities of eucalyptus leaf extracts and flavonoids from eucalyptus maculata. | we investigated the antimicrobial activities of eucalyptus leaf extracts to find effective antibacterial agents. | 2004 | 15189289 |
| design of a 5' exonuclease-based real-time pcr assay for simultaneous detection of bacillus licheniformis, members of the 'b. cereus group' and b. fumarioli in gelatine. | the design of a fast, sensitive and specific detection method for bacillus licheniformis, members of the 'b. cereus group' and b. fumarioli in gelatine. | 2004 | 15189297 |
| sperm bioassay for rapid detection of cereulide-producing bacillus cereus in food and related environments. | a novel in vitro method, sperm micro assay for rapidly distinguishing cereulide, the emetic toxin producing bacillus cereus from non-producers is described and its use for quantitating cereulide and screening large numbers of b. cereus strains/colonies evaluated. the assay is non-laborious and can be executed with equipment present in most laboratories. boar spermatozoa, purchased as standard semen from artificial insemination suppliers, are used to detect toxicity. boar sperms respond within 5 ... | 2004 | 15193804 |
| influence of the cell wall on ciprofloxacin susceptibility in selected wild-type gram-negative and gram-positive bacteria. | the susceptibility of several wild-type bacteria to ciprofloxacin and accumulation of the drug in these bacteria were evaluated. species studied included escherichia coli, serratia marcescens, pseudomonas aeruginosa, staphylococcus aureus, bacillus subtilis and bacillus cereus. ciprofloxacin susceptibility was measured for each strain using two different methods: the minimal inhibitory concentration and the bactericidal index. significant differences were observed between the results derived fro ... | 2004 | 15194135 |
| [biosorption of pb2+ by bacillus cereus biomass]. | in this paper, the potentiometric titration and the sorption experiments were applied to investigate the biosorption behavior of pb2+ on bacillus cereus biomass. it was shown that the biosorption capacities increased with increasing ph from 3 to 7, and the higher concentration of the biomass in solution was favorable to the sorption of pb2+. langmuir model simulated the adsorption data of pb2+ ion very well, and the saturated sorption capacity for pb2+ ion achieved 36.7 mg.g-1 dry biomass. accor ... | 2004 | 15202257 |
| validation of a microbiological method: the star protocol, a five-plate test, for the screening of antibiotic residues in milk. | the results of an in-house laboratory validation of a microbiological method for the screening of antibiotic residues in milk are presented. the sensitivity of this five-plate test, called screening test for antibiotic residues (star), was established by the analysis of milk samples spiked with 66 antibiotics at eight different concentrations. ten different groups of antibiotics were studied: macrolides, aminoglycosides, cephalosporins, penicillins, quinolones, tetracyclines, sulphonamides, linc ... | 2004 | 15204543 |
| identification of sigmab-dependent genes in bacillus cereus by proteome and in vitro transcription analysis. | the alternative sigma factor sigmab of the food pathogen bacillus cereus is activated upon stress exposure and plays a role in the adaptive response of vegetative cells. this study describes the identification of sigmab-dependent genes in b. cereus. two-dimensional gel electrophoresis was performed with protein extracts from a sigmab-overproducing b. cereus strain. nine protein spots, which were absent from the negative control, were identified by matrix-assisted laser desorption ionization-time ... | 2004 | 15205411 |
| anaerobiosis and low specific growth rates enhance hemolysin bl production by bacillus cereus f4430/73. | bacillus cereus f4430/73 produced the highest levels of hemolysin bl (hbl) when grown under anaerobiosis in mod medium. anaerobic cells grown in a chemostat at low specific growth rate (0.1-0.2 h(-1)) expressed up to sevenfold more hbl than did cells held at a faster growth rate. at 0.2 h(-1), the presence of 90 mm glucose resulted in inhibition of hbl production. glucose was found to repress hbl induction at the mrna level, indicating the potential involvement of catabolite repression in the re ... | 2004 | 15205890 |
| bacteriocin-like substance production by bacillus licheniformis strain p40. | to investigate the production of bacteriocin-like compounds by bacillus spp. isolated from the amazon basin. | 2004 | 15214721 |
| pcr detection of bacillus and staphylococcus in various foods. | a broad-range pcr assay for the detection of bacteria belonging to bacillus and staphylococcus genera was developed. primers targeting the bacterial 16s rrna gene were newly designed and used in a pcr assay. to determine the specificity of the assay, 81 different bacterial strains (of 50 genera), 2 fungi, 3 animals, and 4 plants were tested. results were positive for every tested bacillus, staphylococcus, or aerococcus strain. in addition, the result for listeria grayi was positive with lower pc ... | 2004 | 15222564 |
| thermosiphon-based pcr reactor: experiment and modeling. | a self-actuated, flow-cycling polymerase chain reaction (pcr) reactor that takes advantage of buoyancy forces to continuously circulate reagents in a closed loop through various thermal zones has been constructed, tested, and modeled. the heating required for the pcr is advantageously used to induce fluid motion without the need for a pump. flow velocities on the order of millimeters per second are readily attainable. in our preliminary prototype, we measured a cross-sectionally averaged velocit ... | 2004 | 15228345 |
| is231-mic231 elements from bacillus cereus sensu lato are modular. | summary is231a was originally discovered in bacillus thuringiensis as a typical 1.6 kb insertion sequence (is) displaying 20 bp inverted repeats (ir) flanking a transposase gene. a first major variation of this canonical organization was found in mic231a1. this mobile insertion cassette (mic), delineated by is231a-related extremities, contained an active d-stereospecific endopeptidase (adp) gene instead of a transposase. interestingly, it was shown that mic231a1 can be mobilized in trans by the ... | 2004 | 15228527 |
| an effective iodide formulation for killing bacillus and geobacillus spores over a wide temperature range. | to develop a sporicidal reagent which shows potent activity against bacterial spores not only at ambient temperatures but also at low temperatures. | 2004 | 15239708 |
| the antimicrobial activity of extracts of the lichen cladonia foliacea and its (-)-usnic acid, atranorin, and fumarprotocetraric acid constituents. | the antimicrobial activity of the chloroform, diethyl ether, acetone, petroleum ether, and ethanol extracts of the lichen cladonia foliacea and its (-)-usnic acid, atranorin, and fumarprotocetraric acid constituents against 9 bacteria and fungi has been investigated. the extracts and pure compounds alone were found active against the same bacteria and the same yeasts. bacillus cereus, bacillus subtilis, staphylococcus aureus, streptococcus faecalis, proteus vulgaris, listeria monocytogenes, aero ... | 2004 | 15241936 |
| bacteriology of a bear bite wound to a human: case report. | human contact with bears has become more frequent, as has the resultant bear maulings and bite injuries. we report the bacteriology of a patient bitten by a grizzly bear (ursus arctos) from the rocky mountains foothills area east of banff national park, alberta, canada. the patient received field care, including metronidazole and cefazolin. subsequent deep-wound cultures grew serratia fonticola, serratia marcescens, aeromonas hydrophila, bacillus cereus, and enterococcus durans but no anaerobes. | 2004 | 15243122 |
| inhibition of beta-lactamase ii of bacillus cereus by penamaldic derivatives of penicillins. | the penamaldic derivatives of amoxicillin, ampicillin, and penicillins g and v, stabilized with zn(2+), were obtained from a methanolic medium. the enzymatic kinetic results show that the these derivatives elicit reversible inhibition of the enzyme metallo-beta-lactamase from bacillus cereus, with inhibition constant values determined at ph 7.0 and 25 degrees c. | 2004 | 14982810 |
| glycosylphosphatidylinositol (gpi) proteins of saccharomyces cerevisiae contain ethanolamine phosphate groups on the alpha1,4-linked mannose of the gpi anchor. | in humans and saccharomyces cerevisiae the free glycosylphosphatidylinositol (gpi) lipid precursor contains several ethanolamine phosphate side chains, but these side chains had been found on the protein-bound gpi anchors only in humans, not yeast. here we confirm that the ethanolamine phosphate side chain added by mcd4p to the first mannose is a prerequisite for the addition of the third mannose to the gpi precursor lipid and demonstrate that, contrary to an earlier report, an ethanolamine phos ... | 2004 | 14985347 |
| two distinct types of rrna operons in the bacillus cereus group. | the bacillus cereus group includes insecticidal bacteria (b. thuringiensis), food-borne pathogens (b. cereus and b. weihenstephanensis) and b. anthracis, the causative agent of anthrax. the precise number of rrna operons in 12 strains of the b. cereus group was determined. most of the tested strains possess 13 operons and the tested psychrotolerant strains contain 14 operons, the highest number ever found in bacteria. the separate clustering of the tested psychrotolerant strains was confirmed by ... | 2004 | 14993309 |
| evaluation of a new generation of plastic culture bottles with an automated microbial detection system for nine common contaminating organisms found in plt components. | a microbial detection system (bact/alert 3d, biomérieux [formerly organon teknika]) has previously been validated with a variety of bacterial contaminants in plts. the recovery of nine organisms seeded into plts with new plastic culture bottles was studied in comparison to the current glass bottles. the use of plastic instead of glass would be expected to reduce the risk of injury. | 2004 | 14996192 |
| analysis of the substrate specificity loop of the had superfamily cap domain. | the haloacid dehalogenase (had) superfamily includes a variety of enzymes that catalyze the cleavage of substrate c-cl, p-c, and p-op bonds via nucleophilic substitution pathways. all members possess the alpha/beta core domain, and many also possess a small cap domain. the active site of the core domain is formed by four loops (corresponding to sequence motifs 1-4), which position substrate and cofactor-binding residues as well as the catalytic groups that mediate the "core" chemistry. the cap d ... | 2004 | 15005616 |
| characterization of a broad range antimicrobial substance from bacillus cereus. | the aim of this research was to isolate and characterize an antimicrobial substance from the bacillus cereus type strain atcc 14579. | 2004 | 15012801 |
| assessment of bacterial endospore viability with fluorescent dyes. | to validate three fluorescence viability assays designed primarily for vegetative cells on pure bacillus endospores. | 2004 | 15012806 |
| estimating disease resistance in insects: phenoloxidase and lysozyme-like activity and disease resistance in the cricket gryllus texensis. | an animal's ability to resist disease is usually estimated by measuring one or more components of the immune system. there is an assumption that these assays of immunity measure an animal's ability to mount an effective immune response. this paper tests this assumption by examining the relationship between two common estimates of insect immunocompetence, phenoloxidase and lysozyme-like enzyme activity, and resistance to three common insect bacterial pathogens: serratia marcescens, serratia lique ... | 2004 | 15019523 |
| the inhibition of metallo-beta-lactamase by thioxo-cephalosporin derivatives. | the 8-thioxocephalosporins are poor substrates for the b. cereus metallo beta-lactamase (k(cat)/k(m)=61.4m(-1) s(-1)) and act as weak competitive inhibitors (k(i) approximately 700 microm). the hydrolysis product of thioxocephalosporin, a thioacid, also inhibits the enzyme competitively with a k(i)=96 microm, whereas the cyclic thioxo-piperazinedione, formed by intramolecular aminolysis of thioxocephalexin has a k(i) of 29 microm. | 2004 | 15026061 |
| identification of bacillus anthracis specific chromosomal sequences by suppressive subtractive hybridization. | bacillus anthracis, bacillus thuringiensis and bacillus cereus are closely related members of the b. cereus-group of bacilli. suppressive subtractive hybridization (ssh) was used to identify specific chromosomal sequences unique to b. anthracis. | 2004 | 15028116 |
| bacillus anthracis and bacillus cereus pcra helicases can support dna unwinding and in vitro rolling-circle replication of plasmid pt181 of staphylococcus aureus. | replication of rolling-circle replicating (rcr) plasmids in gram-positive bacteria requires the unwinding of initiator protein-nicked plasmid dna by the pcra helicase. in this report, we demonstrate that heterologous pcra helicases from bacillus anthracis and bacillus cereus are capable of unwinding staphylococcus aureus plasmid pt181 from the initiator-generated nick and promoting in vitro replication of the plasmid. these helicases also physically interact with the repc initiator protein of pt ... | 2004 | 15028705 |
| antimicrobial activity of artemisia douglasiana leaf essential oil. | artemisia douglasiana leaf has been shown to be efficacious complementary herbal treatment for chronic bladder infection in a paraplegic youth. the leaf oil has been analyzed by gc-ms and the major components found to be camphor (29%), artemisia ketone (26%), artemisia alcohol (13%), alpha-thujone (10%), 1,8-cineole (8%), and hexanal (5%). the leaf oil and the major components have been tested for antimicrobial activity against bacillus cereus, staphylococcus aureus, escherichia coli, pseudomona ... | 2004 | 15030924 |
| antibacterial screening of plants used in iranian folkloric medicine. | fifty methanolic plant extracts belonging to 44 plant species of 33 families finding use in iranian folkloric medicine were screened for antibacterial activity. thirty samples, including 28 species in 20 families, had antibacterial activity against at least on one of the bacteria. among the active plants, 32.6% were active against g(-), 62% against g(+), and 47.3% against both g(-) and g(+) bacteria. dianthus coryophyllus was active against all tested g(-) and g(+) bacteria except micrococcus lu ... | 2004 | 15030933 |
| identification of emetic toxin producing bacillus cereus strains by a novel molecular assay. | bacillus cereus causes two types of gastrointestinal diseases: emesis and diarrhea. the emetic type of the disease is attributed to the heat-stable depsipeptide cereulide and symptoms resemble staphylococcus aureus intoxication, but there is no rapid method available to detect b. cereus strains causing this type of disease. in this study, a polymerase chain reaction (pcr) fragment of unknown function was identified, which was shown to be specific for emetic toxin producing strains of b. cereus. ... | 2004 | 15033238 |
| rapid dipicolinic acid extraction from bacillus spores detected by surface-enhanced raman spectroscopy. | 2004 | 15035719 | |
| fluorescent amplified fragment length polymorphism analysis of bacillus anthracis, bacillus cereus, and bacillus thuringiensis isolates. | dna from over 300 bacillus thuringiensis, bacillus cereus, and bacillus anthracis isolates was analyzed by fluorescent amplified fragment length polymorphism (aflp). b. thuringiensis and b. cereus isolates were from diverse sources and locations, including soil, clinical isolates and food products causing diarrheal and emetic outbreaks, and type strains from the american type culture collection, and over 200 b. thuringiensis isolates representing 36 serovars or subspecies were from the u.s. depa ... | 2004 | 14766590 |
| development and validation of experimental protocols for use of cardinal models for prediction of microorganism growth in food products. | an experimental protocol to validate secondary-model application to foods was suggested. escherichia coli, listeria monocytogenes, bacillus cereus, clostridium perfringens, and salmonella were observed in various food categories, such as meat, dairy, egg, or seafood products. the secondary model validated in this study was based on the gamma concept, in which the environmental factors temperature, ph, and water activity (aw) were introduced as individual terms with microbe-dependent parameters, ... | 2004 | 14766591 |
| rpob gene sequence-based identification of aerobic gram-positive cocci of the genera streptococcus, enterococcus, gemella, abiotrophia, and granulicatella. | we developed a new molecular tool based on rpob gene (encoding the beta subunit of rna polymerase) sequencing to identify streptococci. we first sequenced the complete rpob gene for streptococcus anginosus, s. equinus, and abiotrophia defectiva. sequences were aligned with these of s. pyogenes, s. agalactiae, and s. pneumoniae available in genbank. using an in-house analysis program (svarap), we identified a 740-bp variable region surrounded by conserved, 20-bp zones and, by using these conserve ... | 2004 | 14766807 |
| identification of proteins in the exosporium of bacillus anthracis. | spores of bacillus anthracis, the causative agent of anthrax, possess an exosporium. as the outer surface layer of these mature spores, the exosporium represents the primary contact surface between the spore and environment/host and is a site of spore antigens. the exosporium was isolated from the endospores of the b. anthracis wild-type ames strain, from a derivative of the ames strain cured of plasmid pxo2(-), and from a previously isolated pxo1(-), pxo2(-) doubly cured strain, b. anthracis um ... | 2004 | 14766913 |
| conjugative transfer, stability and expression of a plasmid encoding a cry1ac gene in bacillus cereus group strains. | the plasmid pht73 containing cry1ac and tagged with an erythromycin resistance gene was transferred from bacillus thuringiensis subspecies kurstaki kt0 to several bacillus cereus group strains by conjugation. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and phase contrast microscopy showed that the transconjugants containing plasmid pht73 could express cry1ac toxin and produce bipyramidal crystalline inclusion bodies during sporulation. the study demonstrated that pht73 c ... | 2004 | 14769465 |
| genetics of zwittermicin a production by bacillus cereus. | zwittermicin a represents a new chemical class of antibiotic and has diverse biological activities, including suppression of oomycete diseases of plants and potentiation of the insecticidal activity of bacillus thuringiensis. to identify genes involved in zwittermicin a production, we generated 4,800 transposon mutants of b. cereus uw101c and screened them for zwittermicin a accumulation. nine mutants did not produce detectable zwittermicin a, and one mutant produced eightfold more than the pare ... | 2004 | 14711631 |
| multilocus sequence typing scheme for bacteria of the bacillus cereus group. | in this study we developed a multilocus sequence typing (mlst) scheme for bacteria of the bacillus cereus group. this group, which includes the species b. cereus, b. thuringiensis, b. weihenstephanensis, and b. anthracis, is known to be genetically very diverse. it is also very important because it comprises pathogenic organisms as well as bacteria with industrial applications. the mlst system was established by using 77 strains having various origins, including humans, animals, food, and soil. ... | 2004 | 14711642 |
| the genome sequence of bacillus cereus atcc 10987 reveals metabolic adaptations and a large plasmid related to bacillus anthracis pxo1. | we sequenced the complete genome of bacillus cereus atcc 10987, a non-lethal dairy isolate in the same genetic subgroup as bacillus anthracis. comparison of the chromosomes demonstrated that b.cereus atcc 10987 was more similar to b.anthracis ames than b.cereus atcc 14579, while containing a number of unique metabolic capabilities such as urease and xylose utilization and lacking the ability to utilize nitrate and nitrite. additionally, genetic mechanisms for variation of capsule carbohydrate an ... | 2004 | 14960714 |
| detection of the bacillus anthracis gyra gene by using a minor groove binder probe. | identification of chromosomal markers for rapid detection of bacillus anthracis is difficult because significant chromosomal homology exists among b. anthracis, bacillus cereus, and bacillus thuringiensis. we evaluated the bacterial gyra gene as a potential chromosomal marker for b. anthracis. a real-time pcr assay was developed for the detection of b. anthracis. after analysis of the unique nucleotide sequence of the b. anthracis gyra gene, a fluorescent 3' minor groove binding probe was tested ... | 2004 | 14715750 |
| making sense of an alphabet soup: the use of a new bioinformatics tool for identification of novel gene islands. focus on "identification of genomic islands in the genome of bacillus cereus by comparative analysis with bacillus anthracis". | 2004 | 14726601 | |
| purification and functional characterization of insecticidal sphingomyelinase c produced by bacillus cereus. | bacillus cereus isolated from the larvae of myrmeleon bore was found to secrete proteins that paralyze and kill german cockroaches, blattela germanica, when injected. one of these active proteins was purified from the culture broth of b. cereus using anion-exchange and gel-filtration chromatography. the purified toxin, with a molecular mass of 34 kda, was identified as sphingomyelinase c (ec 3.1.4.12) on the basis of its n-terminal and internal amino-acid sequences. a recombinant sphingomyelinas ... | 2004 | 14728687 |
| phytoestrogen genistein as an anti-staphylococcal agent. | the soybean-derived isoflavone genistein has been shown to exert beneficial effects on many disorders, including cancer and cardiovascular diseases. the effects of genistein on mammalian cells are mediated by its abilities to inhibit topoisomerase ii and protein tyrosine kinase. in order to examine the potential antibacterial activities of genistein, we incubated the bacteria with various concentrations of this compound for different periods of time and assessed the viable counts. exposure to ge ... | 2004 | 14738897 |
| low molecular weight chitosans: preparation with the aid of papain and characterization. | low molecular weight chitosans (lmwc) of different molecular weight (4.1-5.6 kda) were obtained by the depolymerization of chitosan using papain (from carica papaya latex, ec. 3.4.22.2) at optimum conditions of ph 3.5 and 37 degrees c for 1-5 h. scanning electron microscopy (sem) showed approximately 15-fold decrease in the particle size after depolymerization. decrease in the molecular weight was associated with decrease in the degree of acetylation (da) as evidenced by circular dichroism (cd), ... | 2004 | 14738997 |
| substrate binding to mononuclear metallo-beta-lactamase from bacillus cereus. | structure and dynamics of substrate binding (cefotaxime) to the catalytic pocket of the mononuclear zinc-beta-lactamase from bacillus cereus are investigated by molecular dynamics simulations. the calculations, which are based on the hydrogen-bond pattern recently proposed by dal peraro et al. (j biol inorg chem 2002; 7:704-712), are carried out for both the free and the complexed enzyme. in the resting state, active site pattern and temperature b-factors are in agreement with crystallographic d ... | 2004 | 14747990 |
| reagentless identification of single bacterial spores in aqueous solution by confocal laser tweezers raman spectroscopy. | we demonstrate that optical trapping combined with confocal raman spectroscopy using a single laser source is a powerful tool for the rapid identification of micrometer-sized particles in an aqueous environment. optical trapping immobilizes the particle while maintaining it in the center of the laser beam path and within the laser focus, thus maximizing the collection of its raman signals. the single particle is completely isolated from other particles and substrate surfaces, therefore eliminati ... | 2004 | 14750852 |
| evaluation and establishing the performance of different screening tests for tetracycline residues in animal tissues. | four methods intended for screening muscle tissue for residues belonging to the tetracycline group were compared using artificially contaminated as well as incurred samples. two agar diffusion methods were studied: one with bacillus subtilis as a test strain, the second with bacillus cereus. two variants of each method were compared: thin plates for analysis of intact or minced meat, and thick plates for analysis of meat fluid. the thin plate variants could not be evaluated with artificially con ... | 2004 | 14754636 |
| camelysin is a novel surface metalloproteinase from bacillus cereus. | bacillus cereus frequently causes food poisoning or nosocomial diseases. vegetative cells express the novel surface metalloproteinase camelysin (casein-cleaving metalloproteinase) during exponential growth on complex, peptide-rich media. camelysin is strongly bound to the cell surface and can be solubilized only by detergents or butanol. camelysin spontaneously migrates from the surface of intact bacterial cells to preformed liposomes. the complete sequence of the camelysin-encoding gene, caly, ... | 2004 | 14688099 |
| genotyping of starter cultures of bacillus subtilis and bacillus pumilus for fermentation of african locust bean (parkia biglobosa) to produce soumbala. | bacillus spp. are the predominant microorganisms in fermented african locust bean called soumbala in burkina faso. ten strains selected as potential starter cultures were characterised by pcr amplification of the16s-23s rdna intergenic transcribed spacer (its-pcr), restriction fragment length polymorphism of the its-pcr (its-pcr rflp), pulsed field gel electrophoresis (pfge) and sequencing of the 968-1401 region of the 16s rdna. in previous studies, the isolates were identified by phenotyping as ... | 2004 | 14698101 |
| the alternative sigma factor sigmab of bacillus cereus: response to stress and role in heat adaptation. | a gene cluster encoding the alternative sigma factor sigma(b), three predicted regulators of sigma(b) (rsbv, rsbw, and rsby), and one protein whose function is not known (orf4) was identified in the genome sequence of the food pathogen bacillus cereus atcc 14579. western blotting with polyclonal antibodies raised against sigma(b) revealed that there was 20.1-fold activation of sigma(b) after a heat shock from 30 to 42 degrees c. osmotic upshock and ethanol exposure also upregulated sigma(b), alb ... | 2004 | 14702299 |
| x-ray crystallographic and site-directed mutagenesis analysis of the mechanism of schiff-base formation in phosphonoacetaldehyde hydrolase catalysis. | phosphonoacetaldehyde hydrolase (phosphonatase) catalyzes the hydrolytic p-c bond cleavage of phosphonoacetaldehyde (pald) to form orthophosphate and acetaldehyde. the reaction proceeds via a schiff-base intermediate formed between lys-53 and the pald carbonyl. the x-ray crystal structures of the wild-type phosphonatase complexed with mg(ii) alone or with mg(ii) plus vinylsulfonate (a phosphonoethylenamine analog) were determined to 2.8 and 2.4 a, respectively. these structures were used to dete ... | 2004 | 14670958 |
| development of a rapid method for direct detection of tet(m) genes in soil from danish farmland. | a method for direct detection of antibiotic resistance genes in soil samples has been developed. the tetracycline resistance gene, tet(m), was used as a model. the method was validated on danish farmland soil that had repeatedly been treated with pig manure slurry containing resistant bacteria. the tet(m) gene was directly detected in 10-80% of the samples from the various farmland soils and could be detected in all samples tested after selective enrichment. to validate the obtained results, the ... | 2004 | 14664871 |
| influence of media and temperature on bacteriocin production by bacillus cereus 8a during batch cultivation. | cerein 8a is a bacteriocin produced by the soil bacterium bacillus cereus 8a, isolated from native woodlands of brazil. the influence of temperature and media on the growth of b. cereus 8a and the production of this bacteriocin was studied during batch cultivation. maximum activity was detected by cultivation in brain/heart infusion broth, reaching 3200 activity units ml(-1). bacteriocin was also produced in peptone, mrs, mueller-hinton and nutrient broth, while no activity was observed during c ... | 2004 | 14966665 |
| phosphatidylinositol-specific phospholipase c forms different complexes with monodisperse and micellar phosphatidylcholine. | phosphatidylinositol-specific phospholipase c (pi-plc) from bacillus cereus forms a premicellar complex e(#) with monodisperse diheptanoylphosphatidylcholine (dc(7)pc) that is distinguishable from the e complex formed with micelles. results are interpreted with the assumption that in both cases amphiphiles bind to the interfacial binding surface (i-face) of pi-plc but not to the active site. isothermal calorimetry and fluorescence titration results for the binding of monodisperse dc(7)pc give an ... | 2004 | 14967048 |