Publications

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[molecular methods of detection and identification of pathogenic burkholderia].molecular diagnostic kits for detection and identification of agents of melioidosis and glanders on environmental objects and in clinical material are described. it was demonstrated that pcr with use of specific primers on the basis of different genetic targets could be useful for determination of generic, inter- and intraspecies belonging of pathogenic burkholderia as well as for epidemiologic inspection of territories where melioidosis is enzootic.201021064227
salkowski's reagent test as a primary screening index for functionalities of rhizobacteria isolated from wild dipterocarp saplings growing naturally on medium-strongly acidic tropical peat soil.rhizobacteria isolated from wild dipterocarp saplings in central kalimantan, indonesia, were subjected to salkowski's reagent test, which is often used in detecting indolic substances. among 69 isolates grown in a low-nitrogen medium supplemented with l-tryptophan (trp), culture fluids of 29 strains were positive to the test, in which 17 bacteria turned red and other 10 pink. all the red type rhizobacteria actively converted trp into tryptophol (tol), while some yielded indole-3-acetic acid (iaa ...201021071871
[electricity generation and quinoline degradation of pure strains and mixed strains in the microbial fuel cell].microbial flora composition of microbial fuel cells (mfc) is important to the electricity generation. four bacterium strains q1, b, c and d which represent all different morphology of culturable bacterium were isolated from a mfc using 200 mg x l(-1) quinoline as the fuel and operating for at least 210 days. strains q1, c and d were pseudomonas sp. based on 16s rdna sequence analysis, while strain b was burkholderia sp. double-chamber mfcs using 200 mg x l(-1) quinoline and 300 mg x l(-1) glucos ...201021072938
role of burkholderia pseudomallei in the invasion, replication and induction of apoptosis in human epithelial cell lines.burkholderia pseudomallei is a bacterial pathogen causing melioidosis. in the present study, we demonstrated the effects of b. pseudomallei capsular polysaccharide on the invasion, intracellular replication, induction of cytotoxicity and apoptosis of human epithelial hela and a549 cells. the b. pseudomallei capsule mutant had a significantly greater ability to invade both cell lines (p < 0.05). the b. pseudomallei capsule mutant had a greater ability to induce apoptosis in a549 cells than wild t ...201021073038
antibiosis of burkholderia ubonensis againist burkholderia pseudomallei, the causative agent for melioidosis.melioidosis, caused by burkholderia pseudomallei, is an enigmatic infectious disease that afflicts individuals in many tropical and developing regions. treatment is hampered by the organism's innate antibiotic resistance and the disease's non-pathognomic presentation. recently, added attention has been given to this organism due to its classification as a potential biowarfare agent. therefore, methods of preventing acquisition of infection are needed. we investigated antagonism between burkholde ...201021073065
structural insight into the expanded pcb-degrading abilities of a biphenyl dioxygenase obtained by directed evolution.the biphenyl dioxygenase of burkholderia xenovorans lb400 is a multicomponent rieske-type oxygenase that catalyzes the dihydroxylation of biphenyl and many polychlorinated biphenyls (pcbs). the structural bases for the substrate specificity of the enzyme's oxygenase component (bphae(lb400)) are largely unknown. bphae(p4), a variant previously obtained through directed evolution, transforms several chlorobiphenyls, including 2,6-dichlorobiphenyl, more efficiently than bphae(lb400), yet differs fr ...201021073881
randomized soil survey of the distribution of burkholderia pseudomallei in rice fields in laos.melioidosis is a major cause of morbidity and mortality in southeast asia, where the causative organism (burkholderia pseudomallei) is present in the soil. in the lao people's democratic republic (laos), b. pseudomallei is a significant cause of sepsis around the capital, vientiane, and has been isolated in soil near the city, adjacent to the mekong river. we explored whether b. pseudomallei occurs in lao soil distant from the mekong river, drawing three axes across northwest, northeast, and sou ...201021075883
chromosome translocation and its consequence in the genome of burkholderia cenocepacia au-1054.most bacterial genomes have one single chromosome. the species burkholderia cenocepacia, a gram-negative β-proteobacterium, is one of the exceptions. genomes of four strains of the species have been sequenced and each has three circular chromosomes. in the genus burkholderia, there are another seven sequenced strains that have three chromosomes. in this paper, the numbers of essential genes and trna genes among the 11 strains of the genus burkholderia are compared. interestingly, it is found tha ...201021078295
molecular analysis of antimicrobial agent translocation through the membrane porin bpsomp38 from an ultraresistant burkholderia pseudomallei strain.burkholderia pseudomallei (bps) is a gram-negative bacterium that causes melioidosis, an infectious disease of animals and humans common in northern and north-eastern parts of thailand. successful treatment of melioidosis is difficult due to intrinsic resistance of bps to various antibacterial agents. it has been suggested that the antimicrobial resistance of this organism may result from poor permeability of the active compounds through porin channels located in the outer membrane (om) of the b ...201021078354
size distribution and buoyant density of burkholderia pseudomallei.the size and density of microbial cells determine the time that pathogens can remain airborne and thus, their potential to infect by the respiratory route. we determined the density and size distribution of burkholderia pseudomallei cells in comparison with other burkholderia species, including b. mallei and b. thailandensis, all prepared and analyzed under similar conditions. the observed size distribution and densities of several bacterial strains indicates that aerosolized particles consistin ...201021080156
methods for genetic manipulation of burkholderia gladioli pathovar cocovenenans.abstract:201021080961
nucleotide-binding oligomerization domain-containing protein 2 regulates suppressor of cytokine signaling 3 expression in burkholderia pseudomallei- infected mouse macrophage cell line raw 264.7.burkholderia pseudomallei, a causative agent of melioidosis, is a facultative intracellular gram-negative bacterium that can survive and multiply inside the macrophages. toll-like receptors are one class of pattern recognition receptors (prrs) that have been documented to play significant role in b. pseudomallei infection. in the present study, we investigated a potential role of nucleotide-binding oligomerization domain-containing protein 1 and 2 (nod1 and nod2), cytoplasmic pattern recognition ...201021088051
[burkholderia cepacia lipase gene modification and its constitutive and inducible expression in pichia pastoris].to achieve fast, safe and stable expression of burkholderia cepacia lipase in pichia pastoris.201021090259
novel lytic bacteriophages from soil that lyse burkholderia pseudomallei.burkholderia pseudomallei is a gram-negative saprophytic bacterium that causes severe sepsis with a high mortality rate in humans and a vaccine is not available. bacteriophages are viruses of bacteria that are ubiquitous in nature. several lysogenic phages of burkholderia spp. have been found but information is scarce for lytic phages. six phages, st2, st7, st70, st79, st88 and st96, which lyse b. pseudomallei, were isolated from soil in an endemic area. the phages belong to the myoviridae famil ...201021091532
[a clinical analysis of 25 cases of prosthetic valve endocarditis].to report the clinical characteristics of prosthetic valve endocarditis (pve).201021092446
lung transplantation in cystic fibrosis patients in the czech republic: initial single-center experience.lung transplantation is a well established treatment for advanced lung diseases.201021094844
burkdiff: a real-time pcr allelic discrimination assay for burkholderia pseudomallei and b. mallei.a real-time pcr assay, burkdiff, was designed to target a unique conserved region in the b. pseudomallei and b. mallei genomes containing a snp that differentiates the two species. sensitivity and specificity were assessed by screening burkdiff across 469 isolates of b. pseudomallei, 49 isolates of b. mallei, and 390 isolates of clinically relevant non-target species. concordance of results with traditional speciation methods and no cross-reactivity to non-target species show burkdiff is a robus ...201021103048
pathogen-host interactions in dictyostelium, legionella, mycobacterium and other pathogens.dictyostelium discoideum is a haploid social soil amoeba that is an established host model for several human pathogens. the research areas presently pursued include the use of d. discoideum to identify genetic host factors determining the outcome of infections and the use as screening system for identifying bacterial virulence factors. here we report about the legionella pneumophila directed phagosome biogenesis and the cell-to-cell spread of mycobacterium species. moreover, we highlight recent ...201021109012
challenging and emerging pathogens in cystic fibrosis.cystic fibrosis (cf) lung disease is characterised by chronic inflammation and infection. patients are predominantly infected by specific pathogens, of which staphylococcus aureus and pseudomonas aeruginosa are the most important. in recent years however there has been an increasing number of reports on potentially emerging and challenging pathogens like stenotrophomonas maltophilia, non-tuberculous mycobacteria, highly prevalent p. aeruginosa clones, methicillin resistant staphylococcus aureus ...201021109184
genome wide transcriptome profiling of a murine acute melioidosis model reveals new insights into how burkholderia pseudomallei overcomes host innate immunity.at present, very little is known about how burkholderia pseudomallei (b. pseudomallei) interacts with its host to elicit melioidosis symptoms. we established a murine acute-phase melioidosis model and used dna microarray technology to investigate the global host/pathogen interaction. we compared the transcriptome of infected liver and spleen with uninfected tissues over an infection period of 42 hr to identify genes whose expression is altered in response to an acute infection.201021110886
predation of human pathogens by the predatory bacteria micavibrio aeruginosavorus and bdellovibrio bacteriovorus.the focus of this study was to evaluate the potential use of the predatory bacteria bdellovibrio bacteriovorus and micavibrio aeruginosavorus to control the pathogens associated with human infection.201021114596
effect of colony morphology variation of burkholderia pseudomallei on intracellular survival and resistance to antimicrobial environments in human macrophages in vitro.primary diagnostic cultures from patients with melioidosis demonstrate variation in colony morphology of the causative organism, burkholderia pseudomallei. variable morphology is associated with changes in the expression of a range of putative virulence factors. this study investigated the effect of b. pseudomallei colony variation on survival in the human macrophage cell line u937 and under laboratory conditions simulating conditions within the macrophage milieu. isogenic colony morphology type ...201021114871
burkholderia thailandensis oaca mutants facilitate the expression of burkholderia mallei-like o polysaccharides.previous studies have shown that the o polysaccharides (ops) expressed by burkholderia mallei are similar to those produced by burkholderia thailandensis except that they lack the 4-o-acetyl modifications on their 6-deoxy-α-l-talopyranosyl residues. in the present study, we describe the identification and characterization of an open reading frame, designated oaca, expressed by b. thailandensis that accounts for this phenomenon. utilizing the b. thailandensis and b. mallei lipopolysaccharide (lps ...201021115721
metallo beta-lactamase producing pseudomonas species--a major cause of concern among hospital associated urinary tract infection.multidrug resistant pseudomonas strains are isolated in high frequency from urinary samples in hospitalised patients. with the occurrence of extended spectrum beta-lactamases (esbls) and metallo beta-lactamase (mbl)-producing pseudomonas aeruginosa being increasingly reported worldwide, this study evaluated the resistance pattern of pseudomonad strains to carbapenam and other antipseudomonal antibiotics isolated from patients with hospital associated urinary tract infections along with clinical ...201021121383
virulence of an emerging respiratory pathogen, genus pandoraea, in vivo and its interactions with lung epithelial cells.pandoraea species have emerged as opportunistic pathogens among cystic fibrosis (cf) and non-cf patients. pandoraea pulmonicola is the predominant pandoraea species among irish cf patients. the objective of this study was to investigate the pathogenicity and potential mechanisms of virulence of irish p. pulmonicola isolates and strains from other pandoraea species. three patients from whom the p. pulmonicola isolates were isolated have since died. the in vivo virulence of these and other pandora ...201021127160
dynamics, diversity and function of endophytic siderophore-producing bacteria in rice.siderophore production confers to bacteria competitive advantages to colonize plant tissues and to exclude other microorganisms from the same ecological niche. this work shows that the community of endophytic siderophore-producing bacteria (spb) associated to oryza sativa cultivated in uruguayan soils is dynamic and diverse. these bacteria were present in grains, roots, and leaves, and their density fluctuated between log(10) 3.44 and log(10) 5.52 cfu g(-1) fresh weight (fw) during the plant gro ...201021128071
complete genome sequence of burkholderia rhizoxinica, an endosymbiont of rhizopus microsporus.burkholderia rhizoxinica is an intracellular symbiont of the phytopathogenic fungus rhizopus microsporus. the vertically transmitted endosymbiont not only delivers the antimitotic macrolide rhizoxin to its host but is also essential for vegetative spore formation of the fungus. to shed light on the genetic equipment of this model organism, we sequenced the whole genome of b. rhizoxinica hki 0454, thus providing the first genomic insight into an intracellular mutualist of a fungal species. the 3. ...201021131495
diversity of bacteria associated with bursaphelenchus xylophilus and other nematodes isolated from pinus pinaster trees with pine wilt disease.the pinewood nematode (pwn), bursaphelenchus xylophilus, has been thought to be the only causal agent of pine wilt disease (pwd), however, since bacteria have been suggested to play a role in pwd, it is important to know the diversity of the microbial community associated to it. this study aimed to assess the microbial community associated with b. xylophilus and with other nematodes isolated from pine trees, pinus pinaster, with pwd from three different affected forest areas in portugal. one hun ...201021151611
the epidemiology and clinical spectrum of melioidosis: 540 cases from the 20 year darwin prospective study.over 20 years, from october 1989, the darwin prospective melioidosis study has documented 540 cases from tropical australia, providing new insights into epidemiology and the clinical spectrum.201021152057
diversity of bacterial endophytes in roots of mexican husk tomato plants (physalis ixocarpa) and their detection in the rhizosphere.endophytic bacterial diversity was estimated in mexican husk tomato plant roots by amplified rdna restriction analysis and sequence homology comparison of the 16s rdna genes. sixteen operational taxonomic units from the 16s rdna root library were identified based on sequence analysis, including the classes gammaproteobacteria, betaproteobacteria, actinobacteria, and bacilli. the predominant genera were stenotrophomonas (21.9%), microbacterium (17.1%), burkholderia (14.3%), bacillus (14.3%), and ...201021157706
biofilm-forming bacteria with varying tolerance to peracetic acid from a paper machine.biofilms cause runnability problems in paper machines and are therefore controlled with biocides. peracetic acid is usually effective in preventing bulky biofilms. this study investigated the microbiological status of a paper machine where low concentrations (≤15 ppm active ingredient) of peracetic acid had been used for several years. the paper machine contained a low amount of biofilms. biofilm-forming bacteria from this environment were isolated and characterized by 16s rrna gene sequencing, ...201021161323
the effects of nutrient amendment on biodegradation and cytochrome p450 activity of an n-alkane degrading strain of burkholderia sp. gs3c.the promotion of hexadecane biodegradation activity by an n-alkane degrading strain of burkholderia cepacia (gs3c) with yeast extract amendment was studied using various carbon, nitrogen, vitamin, and amino acid amendments. cytochrome p450 monooxygenase enzymes play a very important role and are especially required to introduce oxygen in n-alkane degradation. these enzymes from gs3c were located and detected using amino acid amendments. it was shown that biodegradation activity was promoted with ...201021167642
in situ evaluation of lipase performances through dynamic asymmetric cyanohydrin resolution.a dynamic resolution process based on multiple reversible cyanohydrin formation coupled to lipase-mediated transesterification is demonstrated. the resulting process resulted in the efficient evaluation of complex lipase performances in asymmetric cyanohydrin acylate synthesis. dynamic systems were generated and resolved in situ, and the effects of the reaction conditions could be directly monitored for the overall system. by this concept, the enzyme activity, chemo- and stereoselectivity for al ...201021170452
identification of immunoreactive secretory proteins from the stationary phase culture of burkholderia pseudomallei.bacterial secreted proteins are known to be involved in virulence and may mediate important host-pathogen interactions. in this study, when the stationary phase culture supernatant of burkholderia pseudomallei was subjected to 2-de, 113 protein spots were detected. fifty-four of the secreted proteins, which included metabolic enzymes, transcription/translation regulators, potential virulence factors, chaperones, transport regulators, and hypothetical proteins, were identified using ms and databa ...201021170972
construction and molecular characterization of mouse single-chain variable fragment antibodies against burkholderia mallei and burkholderia pseudomallei.we have selected two lipopolysaccharide (lps) specific burkholderia mallei mouse monoclonal antibodies (mabs) and four anti-capsular b. pseudomallei-specific mabs to generate mouse single-chain variable fragment (scfv) antibodies. this selection was made through extensive in vitro and in vivo assay from our library of mabs against b. mallei and b. pseudomallei. we initially generated the mouse immunoglobulin variable heavy chain (vh) and light chain (vl) regions from each of these six selected m ...201021172353
a simple and distinctive microbiota associated with honey bees and bumble bees.specialized relationships with bacteria often allow animals to exploit a new diet by providing a novel set of metabolic capabilities. bees are a monophyletic group of hymenoptera that transitioned to a completely herbivorous diet from the carnivorous diet of their wasp ancestors. recent culture-independent studies suggest that a set of distinctive bacterial species inhabits the gut of the honey bee, apis mellifera. here we survey the gut microbiotae of diverse bee and wasp species to test whethe ...201021175905
novel pan-genomic analysis approach in target selection for multiplex pcr identification and detection of burkholderia pseudomallei, burkholderia thailandensis, and burkholderia cepacia complex species: a proof-of-concept study.burkholderia pseudomallei, burkholderia thailandensis, and the burkholderia cepacia complex differ greatly in pathogenicity and epidemiology. yet, they are occasionally misidentified by biochemical profiling, and even 16s rrna gene sequencing may not offer adequate discrimination between certain species groups. using the 23 b. pseudomallei, four b. thailandensis, and 16 b. cepacia complex genome sequences available, we identified gene targets specific to each of them (a tat domain protein, a 70- ...201021177905
dioxygenases in burkholderia ambifaria and yersinia pestis that hydroxylate the outer kdo unit of lipopolysaccharide.several gram-negative pathogens, including yersinia pestis, burkholderia cepacia, and acinetobacter haemolyticus, synthesize an isosteric analog of 3-deoxy-d-manno-oct-2-ulosonic acid (kdo), known as d-glycero-d-talo-oct-2-ulosonic acid (ko), in which the axial hydrogen atom at the kdo 3-position is replaced with oh. here we report a unique kdo 3-hydroxylase (kdoo) from burkholderia ambifaria and yersinia pestis, encoded by the bamb_0774 (bakdoo) and the y1812 (ypkdoo) genes, respectively. when ...201021178073
burkholderia cenocepacia o polysaccharide chain contributes to caspase-1-dependent il-1beta production in macrophages.burkholderia cenocepacia infections in cf patients involve heightened inflammation, fatal sepsis, and high antibiotic resistance. proinflammatory il-1β secretion is important in airway inflammation and tissue damage. however, little is known about this pathway in macrophages upon b. cenocepacia infection. we report here that murine macrophages infected with b. cenocepacia k56-2 produce proinflammatory cytokine il-1β in a tlr4 and caspase-1-mediated manner. we also determined that the ops (o anti ...201021178113
immunospecific responses to bacterial elongation factor tu during burkholderia infection and immunization.burkholderia pseudomallei is the etiological agent of melioidosis, a disease endemic in parts of southeast asia and northern australia. currently there is no licensed vaccine against infection with this biological threat agent. in this study, we employed an immunoproteomic approach and identified bacterial elongation factor-tu (ef-tu) as a potential vaccine antigen. ef-tu is membrane-associated, secreted in outer membrane vesicles (omvs), and immunogenic during burkholderia infection in the muri ...201021179405
cd4+ t-cell immunity to the burkholderia pseudomallei abc transporter lolc in melioidosis.burkholderia pseudomallei causes melioidosis, a disease with a wide range of possible outcomes, from seroconversion and dormancy to sepsis and death. this spectrum of host-pathogen interactions poses challenging questions about the heterogeneity in immunity to b. pseudomallei. models show protection to be dependent on cd4(+) cells and ifn-γ, but little is known about specific target antigens. having previously implicated the abc transporter, lolc, in protective immunity, we here use epitope pred ...201021182082
in vitro activity of doripenem against burkholderia cepacia complex isolates from non-cystic fibrosis patients. 201021189347
a case report of chronic granulomatous disease presenting with aspergillus pneumonia in a 2-month old girl.chronic granulomatous disease (cgd) is an uncommon inherited disorder caused by mutations in any of the genes encoding subunits of the superoxide-generating phagocyte nadph oxidase system, which is essential for killing catalase producing bacteria and fungi, such as aspergillus species, staphylococcus aureus, serratia marcescens, nocardia species and burkholderia cepacia. in case of a history of recurrent or persistent infections, immune deficiency should be investigated. particularly, in the ca ...201021189945
functional quorum sensing systems are maintained during chronic burkholderia cepacia complex infections in patients with cystic fibrosis.quorum sensing (qs) contributes to the virulence of pseudomonas aeruginosa and burkholderia cepacia complex lung infections. p. aeruginosa qs mutants are frequently isolated from patients with cystic fibrosis. the objective of this study was to determine whether similar adaptations occur over time in b. cepacia complex isolates. forty-five burkholderia multivorans and burkholderia cenocepacia sequential isolates from patients with cystic fibrosis were analyzed for n-acyl-homoserine lactone activ ...201021208930
[in vitro antimicrobial activity of piperacillin-sulbactam and other 7 antimicrobial agents against non-fermentative bacilli].to evaluate the in vitro antimicrobial activity of piperacillin-sulbactam against clinical isolates of non-fermentative bacilli isolated from common infections.201021211358
mechanism and inhibition of the fabv enoyl-acp reductase from burkholderia mallei.enoyl-acp reductases catalyze the final step in the elongation cycle of the bacterial fatty acid biosynthesis (fas-ii) pathway. at present, four distinct enoyl-acp reductases have been identified, which are the products of the fabi, fabl, fabk, and fabv genes. the fabv enoyl-acp reductase is the most recent member of this enzyme class and was originally identified in vibrio cholerae by cronan and co-workers [massengo-tiasse, r. p., and cronan, j. e. (2008) vibrio cholerae fabv defines a new clas ...201020055482
determination of diketopiperazines of burkholderia cepacia cf-66 by gas chromatography-mass spectrometry.bacteria communicate with each other by a process termed "quorum sensing" (qs), and diffusible, low-molecular-weight chemicals, called signal molecules, are used as the communication languages. in cell-free burkholderia cepacia cf-66 culture supernatants, five compounds suspected of being signal molecules were identified. the gene (cepi) related with ahls synthesis were not detected by polymerase chain reaction (pcr) using specific primers. gas chromatography-mass spectrometry (gc-ms) revealed t ...201020062980
melioidosis in an urban-dwelling taiwanese man with splenic abscesses.we report a case of melioidosis with splenic abscesses caused by burkholderia pseudomallei in an urban-dwelling, 54-year-old taiwanese man. the patient presented with prolonged fever and abdominal pain. a splenectomy was performed, followed by successful treatment with ceftazidime and amoxicillin-clavulanate. the patient recovered fully.201020709809
actin-based motility of burkholderia thailandensis requires a central acidic domain of bima that recruits and activates the cellular arp2/3 complex.burkholderia species use bima for intracellular actin-based motility. uniquely, burkholderia thailandensis bima harbors a central and acidic (ca) domain. the ca domain was required for actin-based motility, binding to the cellular arp2/3 complex, and arp2/3-dependent polymerization of actin monomers. our data reveal distinct strategies for actin-based motility among burkholderia species.201020693329
detection of quorum sensing molecules in burkholderia cepacia culture supernatants with enzyme-linked immunosorbent assays.the burkholderia cepacia complex (bcc) employs a quorum sensing (qs) mechanism which is a cell density-dependent bacterial communication system to regulate certain gene expressions. as with many other gram-negative bacteria, burkholderia cepacia species use (n-acyl-)homoserine lactones (ahls or hsls) as signalling molecules. because of the essential role of qs in bacterial behavior, the aim of this study was to demonstrate the applicability of our in-house-developed enzyme-linked immunosorbent a ...201020694722
sodium nitrite-mediated killing of the major cystic fibrosis pathogens pseudomonas aeruginosa, staphylococcus aureus, and burkholderia cepacia under anaerobic planktonic and biofilm conditions.a hallmark of airways in patients with cystic fibrosis (cf) is highly refractory, chronic infections by several opportunistic bacterial pathogens. a recent study demonstrated that acidified sodium nitrite (a-no(2)(-)) killed the highly refractory mucoid form of pseudomonas aeruginosa, a pathogen that significantly compromises lung function in cf patients (s. s. yoon et al., j. clin. invest. 116:436-446, 2006). therefore, the microbicidal activity of a-no(2)(-) (ph 6.5) against the following thre ...201020696868
case-crossover study of burkholderia cepacia complex bloodstream infection associated with contaminated intravenous bromopride.to investigate an outbreak of healthcare-associated burkholderia cepacia complex (bcc) primary bloodstream infections (bcc-bsi).201020205589
the burkholderia cenocepacia k56-2 pleiotropic regulator pbr, is required for stress resistance and virulence.burkholderia cenocepacia is one of the most virulent species of the burkholderia cepacia complex, a group of bacteria that emerged as important pathogens, especially to cystic fibrosis (cf) patients. in this study, we report the identification and characterization of a mutant strain derived form the cf isolate burkholderia cenocepacia k56-2, carrying a plasposon insertion in a gene, located in a 3516 bp chromosomal region with an atypical g+c content, encoding a 80 amino acid putative regulatory ...201020206249
degradation of parabens by pseudomonas beteli and burkholderia latens.p-hydroxybenzoic acid esters (parabens) are commonly used antimicrobial preservatives in pharmaceutical formulations. two microorganisms, isolated from non-sterile methyl paraben (mp) and propyl paraben (pp) solutions, were found to degrade the respective parabens. identification by 16s rrna partial gene sequencing revealed them to be pseudomonas beteli and burkholderia latens, respectively. the present work describes a previously unreported interaction of the parabens with p. beteli and b. late ...201020206257
comparison of 16s rrna gene phylogeny and functional tfda gene distribution in thirty-one different 2,4-dichlorophenoxyacetic acid and 4-chloro-2-methylphenoxyacetic acid degraders.31 different bacterial strains isolated using the herbicide 2,4-dichlorophenoxyacetic acid (2,4-d) as the sole source of carbon, were investigated for their ability to mineralize 2,4-d and the related herbicide 4-chloro-2-methylphenoxyacetic acid (mcpa). most of the strains mineralize 2,4-d considerably faster than mcpa. three novel primer sets were developed enabling amplification of full-length coding sequences (cds) of the three known tfda gene classes known to be involved in phenoxy acid deg ...201020206455
cloning, expression, purification, crystallization and preliminary crystallographic studies of bcec, a udp-glucose dehydrogenase from burkholderia cepacia ist408.bacteria of the burkholderia cepacia complex (bcc) have emerged as important opportunistic pathogens, establishing lung infections in immunocompromised or cystic fibrosis patients. bcc uses polysaccharide-biofilm production in order to evade the host immune response. the biofilm precursor udp-glucuronic acid is produced by a twofold nad(+)-dependent oxidation of udp-glucose. in b. cepacia ist408 this enzymatic reaction is performed by the udp-glucose dehydrogenase bcec, a 470-residue enzyme, the ...201020208157
[microbial population of lower respiratory tract in children from different age groups with cystic fibrosis].to study microflora of lower respiratory tract of children from different age groups with cystic fibrosis during follow-up for determination of its variability and possible sources of infectious complications.201020218338
melioidosis and the vacuum-assisted closure device: a rare cause of a discharging neck wound, and a new approach to management.we report a case of melioidosis presenting as a discharging neck abscess, and we describe the use of a vacuum-assisted closure device in its management.201020219147
role of a burkholderia pseudomallei polyphosphate kinase in an oxidative stress response, motilities, and biofilm formation.burkholderia pseudomallei, a motile and rod gram-negative bacterium, is the causative agent of melioidosis. the bacterium is an intracellular pathogen and that motility is generally crucial for their survival in a natural environment and for systemic infection inside a host. we report here a role of b. pseudomallei polyphosphate kinase in virulence, such as an oxidative stress response, motilities and biofilm formation. the polyphosphate kinase (ppk) mutant is susceptible to hydrogen peroxide in ...201020221731
evaluating the performance of oligonucleotide microarrays for bacterial strains with increasing genetic divergence from the reference strain.dna oligonucleotide microarrays (oligoarrays) are being developed continuously; however, several issues regarding the applicability of these arrays for whole-genome dna-dna strain comparisons (genomotyping) have not been investigated. for example, the extent of false negatives (i.e., no hybridization signal is observed when the amino acid sequence is conserved but the nucleotide sequence has diverged to a level that does not allow hybridization) remains speculative. to provide quantitative answe ...201020228107
insight into the structure of an endopolygalacturonase from the phytopathogen burkholderia cepacia: a biochemical and computational study.we have recently investigated and characterized the mode of action of bcpeh28a, an endopolygalacturonase (endopg) from the phytopathogen burkholderia cepacia. endopgs belong to glycoside hydrolase family 28 and are responsible for the hydrolysis of the non-esterified regions of pectins. here we report a 3-d structural model of bcpeh28a by combining mass spectrometry (ms) analysis, aimed at disulphide bridges mapping, and computational modelling tools. ms analyses have revealed the complete patte ...201020637827
construction of an artificial microalgal-bacterial consortium that efficiently degrades crude oil.four oil component-degrading bacteria and one oil-tolerant microalgae, scenedesmus obliquus gh2, were used to construct an artificial microalgal-bacterial consortium for crude-oil degradation. the bacterial strains included sphingomonas gy2b and burkholderia cepacia gs3c, along with a mixed culture, named gp3, containing pseudomonas gp3a and pandoraea pnomenusa gp3b. gy2b could only degrade polycyclic aromatic hydrocarbons, gs3c was able to degrade aliphatic chain hydrocarbons, and gp3 could uti ...201020638971
a decade of burkholderia cenocepacia virulence determinant research.the burkholderia cepacia complex (bcc) is a group of genetically related environmental bacteria that can cause chronic opportunistic infections in patients with cystic fibrosis (cf) and other underlying diseases. these infections are difficult to treat due to the inherent resistance of the bacteria to antibiotics. bacteria can spread between cf patients through social contact and sometimes cause cepacia syndrome, a fatal pneumonia accompanied by septicemia. burkholderia cenocepacia has been the ...201020643851
computerized tomographic findings of hepatic fascioliasis compared with melioidosis-caused liver abscesses.to compare the computerized tomographic (ct) findings of hepatic fascioliasis (hf) vs. melioidosis-caused liver (ml) abscesses.201020649065
identification of species of the burkholderia cepacia complex by sequence analysis of the hisa gene.bacteria of the burkholderia cepacia complex (bcc) are opportunistic human pathogens that can cause serious infections in the lungs of cystic fibrosis patients. the bcc is a complex taxonomic group and comprises 17 closely related species of both biotechnological and clinical importance that have been discriminated by a polyphasic taxonomic approach. in this study we focused on the hisa gene, which encodes a 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamid ...201020651037
microbial contamination of disinfectants used for intermittent self-catheterization.we investigated the microbial contamination of 0.02% benzalkonium chloride solution used in catheter kits for intermittent self-catheterization. of 20 samples examined, 12 (60.0%) were contaminated with 8.8 x 10(2)-3.1 x 10(6) colony-forming units (cfu)/ml. the contaminants were pseudomonas fluorescens, burkholderia cepacia, and aeromonas spp. these results showed that 0.02% benzalkonium chloride solution used for the lubrication/disinfection of catheters for self-catheterization is susceptible ...201020657069
defining routes of airborne transmission of pseudomonas aeruginosa in people with cystic fibrosis.pseudomonas aeruginosa is a common and important pathogen in people with cystic fibrosis (cf). with the advent of modern genotyping, a number of clonal strains of p. aeruginosa have been identified, some of which are associated with increased morbidity. the route of cross-infection between people with cf is not clear, but there is evidence that an airborne route may be important. laboratory studies have shown that p. aeruginosa can survive within droplet nuclei and can potentially remain suspend ...201020658913
the type 2 secretion pseudopilin, gspj, is required for multihost pathogenicity of burkholderia cenocepacia au1054.burkholderia cenocepacia au1054 is an opportunistic pathogen isolated from the blood of a person with cystic fibrosis. au1054 is a multihost pathogen causing rapid pathogenicity to caenorhabditis elegans nematodes. within 24 h, au1054 causes greater than 50% mortality, reduced growth, emaciated body, distended intestinal lumen, rectal swelling, and prolific infection of the nematode intestine. to determine virulence mechanisms, 3,000 transposon mutants were screened for attenuated virulence in n ...201020660607
genetic and phenotypic diversity in burkholderia: contributions by prophage and phage-like elements.burkholderia species exhibit enormous phenotypic diversity, ranging from the nonpathogenic, soil- and water-inhabiting burkholderia thailandensis to the virulent, host-adapted mammalian pathogen b. mallei. genomic diversity is evident within burkholderia species as well. individual isolates of burkholderia pseudomallei and b. thailandensis, for example, carry a variety of strain-specific genomic islands (gis), including putative pathogenicity and metabolic islands, prophage-like islands, and pro ...201020667135
straightforward synthesis of enantiopure 2,3-dihydrobenzofurans by a sequential stereoselective biotransformation and chemical intramolecular cyclization.a new family of optically active 2,3-dihydrobenzofurans has been prepared by a simple chemoenzymatic asymmetric strategy. this synthetic approach is based on the combination of a lipase-mediated kinetic resolution of 1-aryl-2-propanols or bioreduction of the corresponding ketones followed by an intramolecular cyclization reaction. these novel compounds have been prepared in enantiopure form and in good overall yield through a straightforward route.201020670014
the role of mucoidy in virulence of bacteria from the burkholderia cepacia complex: a systematic proteomic and transcriptomic analysis.bacteria of the burkholderia cepacia complex (bcc) are associated with severe infection in cystic fibrosis. recent evidence shows that the mucoid phenotype is common in bcc bacteria; however, during chronic infection, transitions from the mucoid to nonmucoid morphology have been shown to take place. here we use rna microarray and proteomic isobaric tagging relative and absolute quantitation technologies to gain insight into a pair of mucoid and nonmucoid isolates of b. cenocepacia obtained from ...201020670172
gender differences in the scandinavian cystic fibrosis population.to explore whether gender differences in the scandinavian cystic fibrosis (cf) patients exist in the areas of key clinical parameters, complications, and medication.201020672360
antimicrobial activities of ll-37 and its truncated variants against burkholderia thailandensis.antimicrobial peptides (amps) are essential host defence molecules found in a wide variety of species and are promising antibacterial therapeutic candidates. focusing on the human cathelicidin peptide ll-37, the aim of the present study was to explore the mechanisms of action and antimicrobial activities of a library of ll-37 fragments using burkholderia thailandensis e264 as a model. the results revealed that ig-19 was the shortest fragment within ll-37 that exhibited antibacterial activity. ll ...201020685090
[biodiversity of phosphate-dissolving and plant growth--promoting endophytic bacteria of two crops].we isolated and characterized phosphate-dissolving endophytic bacteria from two commonly cultivated crops.201020687333
glutamine deamidation and dysfunction of ubiquitin/nedd8 induced by a bacterial effector family.a family of bacterial effectors including cif homolog from burkholderia pseudomallei (chbp) and cif from enteropathogenic escherichia coli (epec) adopt a functionally important papain-like hydrolytic fold. we show here that chbp was a potent inhibitor of the eukaryotic ubiquitination pathway. chbp acted as a deamidase that specifically and efficiently deamidated gln40 in ubiquitin and ubiquitin-like protein nedd8 both in vitro and during burkholderia infection. deamidated ubiquitin was impaired ...201020688984
characterisation and evaluation of synthetic antimicrobial peptides against bacillus globigii, bacillus anthracis and burkholderia thailandensis.antimicrobial peptides (amps) are produced by all forms of living organisms and represent a novel class of antibiotics to treat infectious diseases. in this study, 29 amps of varying length and characteristics were synthesised chemically and were evaluated for their ability to inhibit the growth of bacillus globigii, bacillus anthracis and burkholderia thailandensis. amongst the peptides tested, sheep myeloid antimicrobial peptide-29 (smap-29) was the most potent, inhibiting both b. globigii and ...201020692130
burkholderia cepacia complex in septicaemic non-cystic fibrosis cases from two tertiary care hospitals in north india. 201020571175
a high-throughput pipeline for the design of real-time pcr signatures.pathogen diagnostic assays based on polymerase chain reaction (pcr) technology provide high sensitivity and specificity. however, the design of these diagnostic assays is computationally intensive, requiring high-throughput methods to identify unique pcr signatures in the presence of an ever increasing availability of sequenced genomes.201020573238
long-term stability of temocillin in elastomeric pumps for outpatient antibiotic therapy in cystic fibrosis patients. 201020576638
spectrum of melioidosis in the suburbs of mangalore, s west coast of india.melioidosis is an emerging infection in india. seventeen cases of culture proven melioidosis are reported in this study. the isolation rate was high during the rainy season. except one case, all the patients had diabetes mellitus as an underlying disease. eleven patients improved with ceftazidime or combination therapy and maintenance therapy with doxycycline and cotrimoxazole. the high prevalence of b. pseudomallei in this region is a matter for serious concern.201020578496
burkholderia thailandensis whole cell antigen cross-reacts with b. pseudomallei antibodies from patients with melioidosis in an immunofluorescent assay.an immunofluorescent assay (ifat) using whole cell antigen derived from burkholderia thailandensis used for detection of total antibodies to burkholderia pseudomallei, was found to compare favorably with a previous published report on a b. pseudomallei ifat assay. at a 1:20 cut-off titer, the assay had high sensitivity (98.9%) and satisfactory specificity (92.3%), when tested against sera from 94 patients suspected of melioidosis. sera from 12 patients with culture proven melioidosis gave absolu ...201020578523
melioidosis in southern india: epidemiological and clinical profile.melioidosis, which is mainly prevalent in thailand and australia, has shown an increasing trend in india in the last few years. we carried out a retrospective study of 25 culture-proven adult cases of melioidosis who were admitted to a tertiary care hospital in southern india during june 2001 to september 2007. there was a six-fold increase in the number of cases in 2006 and 2007 as compared to 2001. diabetes mellitus was the predisposing factor in 68% of cases, followed by alcoholism (28%). the ...201020578524
high-cell-density poly (3-hydroxybutyrate) production from sucrose using burkholderia sacchari culture in airlift bioreactor.burkholderia sacchari ipt 189 poly (3-hydroxybutyrate) (p3hb) production in airlift bioreactor were investigated in batch and fed-batch culture using sucrose as carbon source. in batch experiments it was observed that during the growth phase b. sacchari ipt 189 might display exponential growth even at very low carbohydrate concentration, as long as nh(4)(+) concentration was above 190 mg l(-1). the onset of accumulation phase took place when nh(4)(+) concentration dropped below this value and co ...201020580221
melioidosis and pulmonary tuberculosis co-infection in a diabetic.burkholderia pseudomallei is the causative agent of melioidosis. it is endemic in south east asian countries and north australia. sporadic cases of melioidosis have been reported from several parts of south india. melioidosis may manifest as chronic pneumonia mimicking tuberculosis and generally be seen as a single entity. we report the first case of melioidosis and pulmonary tuberculosis co-infection in a diabetic patient. the causative agents were identified using standard methods and the pati ...201020582178
escape of intracellular shigella from autophagy requires binding to cholesterol through the type iii effector, icsb.type iii secretion systems are present in many pathogenic bacteria and mediate the translocation of bacterial effectors into host cells. identification of host targets of these effectors is crucial for understanding bacterial virulence. icsb, a type iii secretion effector, helps shigella to evade the host autophagy defense system by binding to the autophagy protein, atg5. here, we show that icsb is able to interact specifically with cholesterol. the cholesterol binding domain (cbd) of icsb is lo ...201020599519
macrophage responses to cf pathogens: jnk map kinase signaling by burkholderia cepacia complex lipopolysaccharide.chronic bacterial colonization of the airways with opportunistic pathogens is the primary cause of morbidity and mortality in cystic fibrosis (cf) patients. burkholderia cepacia complex (bcc) organisms pose a particular challenge in cf lung disease, due in part to their ability to trigger a fulminant pneumonia. this study compares the u937 macrophage response to two bcc species, b. cenocepacia and burkholderia multivorans, against pseudomonas aeruginosa and staphylococcus aureus. the two bcc str ...201020602636
burkholderia pseudomallei rpos regulates oxyr and the katg-dpsa operon under conditions of oxidative stress.burkholderia pseudomallei, the causative agent of the potentially fatal tropical disease melioidosis, is known to be highly resistant to oxidative stress although the mechanism of this resistance remains to be fully elucidated. previous studies have shown that an oxyr is involved in the regulation of oxidative stress via the katg and dpsa genes encoding katg and dpsa and that the alternative sigma factor, rpos, plays a critical role in resistance to oxidative stress by regulating katg and kate g ...201020618685
identification of putative biomarkers for toluene-degrading burkholderia and pseudomonas by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and peptide mass fingerprinting.the use of peptide mass fingerprinting with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) was demonstrated to identify and phenotypically characterize toluene-degrading bacteria via biomarkers of degradation and taxonomical classification. pseudomonas putida f1, p. mendocina kr1, and burkholderia sp. js150 were grown on toluene, extracted, electrophoretically separated, and analyzed by maldi-tof ms. catabolic enzymes were identified and results subst ...201020622441
a sensor kinase recognizing the cell-cell signal bdsf (cis-2-dodecenoic acid) regulates virulence in burkholderia cenocepacia.burkholderia cenocepacia is an opportunistic human pathogen that uses cis-2-dodecenoic acid (bdsf) as a quorum-sensing signal to control expression of virulence factors. bdsf is a signal molecule of the diffusible signal factor (dsf) family that was first described in the plant pathogen xanthomonas campestris. the mechanism of perception of this signal and the range of functions regulated in b. cenocepacia are, however, unknown. a screen for transposon mutants unable to respond to exogenous sign ...201020624216
social interactions in the burkholderia cepacia complex: biofilms and quorum sensing.burkholderia cepacia complex bacteria are opportunistic pathogens that cause respiratory tract infections in susceptible patients, mainly people with cystic fibrosis. there is convincing evidence that b. cepacia complex bacteria can form biofilms, not only on abiotic surfaces (e.g., glass and plastics), but also on biotic surfaces such as epithelial cells, leading to the suggestion that biofilm formation plays a key role in persistent infection of cystic fibrosis lungs. this article presents an ...201020632807
solution conformation and dynamics of exopolysaccharides from burkholderia species.exopolysaccharides (epss) from the burkholderia genus are proposed to be involved in pathological conditions in humans, such as cystic fibrosis and septicemia, as well as in the stability of soil aggregates. hence, considering that the conformational and dynamic aspects of such epss may influence their biological activity, the current work employs a series of molecular dynamics simulations on di-, oligo-, and polysaccharide fragments of three epss, from burkholderia caribensis, burkholderia cepa ...201020633874
drosophila melanogaster as a model host for the burkholderia cepacia complex.colonization with bacterial species from the burkholderia cepacia complex (bcc) is associated with fast health decline among individuals with cystic fibrosis. in order to investigate the virulence of the bcc, several alternative infection models have been developed. to this end, the fruit fly is increasingly used as surrogate host, and its validity to enhance our understanding of host-pathogen relationships has been demonstrated with a variety of microorganisms. moreover, its relevance as a suit ...201020635002
response of sessile cells to stress: from changes in gene expression to phenotypic adaptation.a better understanding of the genotypic and phenotypic adaptation of sessile (biofilm-associated) microorganisms to various forms of stress is required in order to develop more effective antibiofilm strategies. this review presents an overview of what high-throughput transcriptomic analyses have taught us concerning the response of various clinically relevant microorganisms (including pseudomonas aeruginosa, burkholderia cenocepacia and candida albicans) to treatment with antibiotics or disinfec ...201020482621
detection of hyphomycetes in the upper respiratory tract of patients with cystic fibrosis.the respiratory tract of cystic fibrosis patients is colonised by bacteria and fungi. although colonisation by slow growing fungi such as pseudallescheria, scedosporium and exophiala species has been studied previously, the colonisation rate differs from study to study. infections caused by these fungi have been recognised, especially after lung transplants. monitoring of respiratory tract colonisation in cystic fibrosis patients includes the use of several semi-selective culture media to detect ...201020492536
morphological changes of fusarium oxysporum induced by cf66i, an antifungal compound from burkholderia cepacia.the morphological effects of cf66i, an antifungal compound produced by burkholderia cepacia, on growing hyphae of fusarium oxysporum were studied by fluorescence microscopy (fm) and transmission electron microscopy (tem). at 20 μg/ml, cf66i strongly inhibited growth and induced significant changes of the hyphal morphology. these changes included swelling of hyphae with considerable thickening cell wall and abnormal chitin deposition, which was indicative of the alterations in cell wall structure ...201020495943
the bpeab-oprb efflux pump of burkholderia pseudomallei 1026b does not play a role in quorum sensing, virulence factor production, or extrusion of aminoglycosides but is a broad-spectrum drug efflux system.most burkholderia pseudomallei strains are intrinsically aminoglycoside resistant, mainly due to amrab-opra-mediated efflux. rare naturally occurring or genetically engineered mutants lacking this pump are aminoglycoside susceptible despite the fact that they also encode and express bpeab-oprb, which was reported to mediate efflux of aminoglycosides in the singapore strain khw. to reassess the role of bpeab-oprb in b. pseudomallei aminoglycoside resistance, we used mutants overexpressing or lack ...201020498323
miniemulsion as efficient system for enzymatic synthesis of acid alkyl esters.the aim of this work is to devise an efficient enzymatic process for the production of linear alkyl esters in aqueous miniemulsion systems. the esterification reactions of linear alcohols and carboxylic acids were performed with three different enzymes, commercial amano lipase ps from pseudomonas cepacia, lipase type vii from candida rugosa, and lyophilized fusarium solani pisi cutinase expressed in saccharomyces cerevisiae su50. the miniemulsion system shows a high potential for the synthesis o ...201020503297
structural and functional characterization of diffusible signal factor family quorum-sensing signals produced by members of the burkholderia cepacia complex.previous work has shown that burkholderia cenocepacia produces the diffusible signal factor (dsf) family signal cis-2-dodecenoic acid (c(12):delta(2), also known as bdsf), which is involved in the regulation of virulence. in this study, we determined whether c(12):delta(2) production is conserved in other members of the burkholderia cepacia complex (bcc) by using a combination of high-performance liquid chromatography, mass spectrometry, and bioassays. our results show that five bcc species are ...201020511428
influence of forest trees on the distribution of mineral weathering-associated bacterial communities of the scleroderma citrinum mycorrhizosphere.in acidic forest soils, availability of inorganic nutrients is a tree-growth-limiting factor. a hypothesis to explain sustainable forest development proposes that tree roots select soil microbes involved in central biogeochemical processes, such as mineral weathering, that may contribute to nutrient mobilization and tree nutrition. here we showed, by combining soil analyses with cultivation-dependent analyses of the culturable bacterial communities associated with the widespread mycorrhizal fung ...201020511429
antimicrobial activity of chir-090, an inhibitor of lipopolysaccharide biosynthesis, against the burkholderia cepacia complex. 201020516283
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