Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| human lymphoblastoid cell line producing protective monoclonal igg1, kappa anti-tetanus toxin. | peripheral blood lymphocytes from an individual, recently boosted with tetanus toxoid (tt), were transformed with epstein-barr virus. no antigen-specific selection nor stimulation of b cells was performed prior to transformation. one stable cell line, designated clb-hu-tt-1, was established. this cell line has a doubling time of 24 h and yields 10 micrograms/ml of a monoclonal igg1, kappa anti-tt antibody in bulk cultures. the antibody is biologically active in that it can protect mice against t ... | 1984 | 6327321 |
| analysis of human monoclonal antibodies derived from lymphocytes of patients with cancer. | human lymphocytes from tumor-bearing patients and normal individuals have been fused with the ns-1 mouse myeloma line or the licr -lon- hmy2 ( licr -2) or sk0 -007 human cell lines. for a given number of lymphocytes, fusions with ns-1 produced 8 times more clones than fusions with licr -2 and greater than 20 times more clones than fusions with sk0 -007. the percentage of clones that secrete human immunoglobulin (ig) and the range of ig production were comparable for clones derived from the three ... | 1984 | 6327400 |
| expression in e. coli of a fusion protein encoded by parts of the sv40 and ebv genomes. | 1984 | 6327408 | |
| downregulation occurs normally in cultured epstein-barr virus-transformed lymphocytes from patients with extreme insulin resistance. discrepancy between downregulation in vivo and in vitro. | levels of fasting plasma insulin are generally inversely correlated with 125i-insulin binding to circulating blood cells. in disease states associated with hyperinsulinemia (e.g., obesity and non-insulin-dependent diabetes mellitus), 125i-insulin binding is usually low. in contrast, 125i-insulin binding to circulating cells may be normal in patients with certain forms of extreme insulin resistance despite marked hyperinsulinemia. to explain this paradox, it has been proposed that postbinding def ... | 1984 | 6327434 |
| antibody-dependent cell-mediated cytotoxicity against hla-a, hla-b and hla-dr specificities. | hla specificity of alloantibodies involved in antibody-dependent cell-mediated cytotoxicity (adcc) was investigated using pha-induced lymphoblasts ( plb ) from peripheral blood and epstein-barr virus-induced human lymphoblastoid cell lines (lcl). the adcc activity with plb sensitized by hla-a or hla-b antibodies was relatively low, but higher than that of control. dr antibodies completely failed to sensitize plb for adcc. lcl sensitized by corresponding dr antibodies gave strong adcc reactions, ... | 1984 | 6327536 |
| humoral immune response to epstein-barr virus antigens and immunoglobulin allotypes in african burkitt lymphoma patients. | we examined whether any immunoglobulin allotype was associated with an elevated risk of burkitt's lymphoma because of the association between burkitt's lymphoma and abnormalities of the immunoglobulin-bearing chromosomes. the distribution of gm and km phenotypes among 56 burkitt lymphoma cases and 25 age-matched controls was unremarkable. burkitt's lymphoma has also been associated with the epstein-barr virus, and we therefore sought a relationship between immunoglobulin allotypes and antibody r ... | 1984 | 6327537 |
| calcium concentration defines two stages in transformation of lymphocytes by epstein-barr virus. | lymphocytes from epstein-barr virus (ebv) seronegative donors were either stimulated with protein a or infected with ebv and cultured in growth medium containing a range of calcium levels (700 microm to less than 2 microm available calcium). three calcium end-points for the proliferation of b lymphocytes were defined: 18 microm for mitogenically-stimulated b cells; 8 microm for a pre-12 h event in ebv transformation and less than 2 microm for a post-12 h event in ebv transformation resembling th ... | 1984 | 6327539 |
| properties of immunotoxins against a glycolipid antigen associated with burkitt's lymphoma. | a monoclonal immunoglobulin m (igm) antibody (38-13) which recognizes burkitt's lymphoma (bl) cells, by reacting with the neutral glycolipid gal alpha 1 leads to 4-gal beta 1 leads to 4-glc beta 1 leads to 1-ceramide, was recently characterized. this monoclonal igm was coupled to either ricin a chain or gelonin. the two different immunotoxins obtained retained the apparent immunological specificity of 38-13 igm, as shown by flow cytofluorometry analysis and complement-dependent cytotoxicity test ... | 1984 | 6690030 |
| spontaneous clearance and reactivation of hepatitis b virus infection among male homosexuals with chronic type b hepatitis. | abrupt increases of alanine transaminase were observed in 6 of 23 non-treated, male homosexuals with chronic hepatitis associated with hepatitis b virus. before this occurrence, all subjects had hepatitis b e antigen (hbeag) and elevated dna polymerase activity. within 3 months, hbeag was nondetectable in 3 subjects and elevated dna polymerase disappeared in 4. these serologic events were not always sustained, however. in 3 subjects, reactivation of hepatitis b virus infection occurred within th ... | 1984 | 6691658 |
| is cytoplasmic ca2+ in lymphocytes elevated in cystic fibrosis? | an increased cytoplasmic ca2+ concentration ( [ca2+]i) has been implicated in the pathogenesis of cystic fibrosis. we compared the [ca2+]i levels of normal and cystic fibrosis peripheral blood lymphocytes and epstein-barr virus-transformed lymphoblasts using quin 2, an internally trapped indicator. the [ca2+]i levels of normal and cystic fibrosis cells were not significantly different. the ionophore-releasable intracellular ca2+ stores were also comparable in both types of individual. | 1984 | 6691977 |
| acute psychosis in a 45-year-old man with bipolar disorder and primary epstein-barr virus infection: a case report. | a 45-year-old physician with bipolar disorder presented with an acute organic psychosis, fever, and hematologic and serologic findings of a primary epstein-barr virus infection. pathogenesis was complicated by the history of discontinued lithium therapy prior to the psychosis. the patient recovered completely. literature concerning psychosis and infectious mononucleosis is reviewed. | 1984 | 6693024 |
| infectious mononucleosis in greenland: a disease of the non-indigenous population. | greenland eskimos have one of the highest incidences of nasopharyngeal carcinoma (npc) in the world. asian and african areas endemic for npc show early infection with epstein-barr virus (ebv), and few cases of infectious mononucleosis (im). among 40 000 eskimos and 10 000 caucasians in greenland there were 82 reported cases of im from 1963 to 1980. incidence rates from 1975 to 1980, age adjusted to the "world" population distribution, were 12.0 and 5.2/100 000/yr for males and females, respectiv ... | 1984 | 6695160 |
| detection of mycoplasma contamination lymphoblastoid cell lines by monoclonal antibodies. | we have produced two monoclonal antibodies, sfr1-myco 1 and sfr1-myco 2, that detect mycoplasma fermentans found to contaminate lymphoblastoid cell lines (lcl). the specificity of these monoclonal antibodies against the m. fermentans was determined by indirect immunofluorescence by demonstrating the reactivity of the monoclonal antibodies with known reference strains of mycoplasma grown on soft agar. the reactivity of these antibodies against lcl in a number of immunoassays correlates completely ... | 1984 | 6698819 |
| sequential changes of the five immunoglobulin classes and other responses in infectious mononucleosis. | serum total igg, iga, igm, igd, and ige concentrations, heterophil antibody titer, epstein-barr virus (ebv)-specific antibodies titer, and hematologic changes were studied longitudinally in 19 patients with ebv-induced infectious mononucleosis who were followed up for 1.5-34 months. in 9 patients, the changes in these variables were compared with baseline data on a pre-illness serum sample. all 5 immunoglobulins (ig) showed a significant rise during the acute illness followed by a drop during co ... | 1984 | 6706423 |
| characterization of a monoclonal antibody that reacts with activated/proliferating cells and subsets of leukemia cells. | this report describes a murine igg2a monoclonal antibody, called l22, derived by immunizations with an epstein-barr virus-negative large cell lymphoma b cell line. the antigen detected by l22 is not present on normal peripheral blood cells, but is present on cells stimulated by various mitogens. the proportion of l22+ cells correlates closely with blastogenesis and 125i-uridine uptake. l22 precipitates a 89,500-dalton antigen under reducing conditions, and a 180,000-dalton antigen under nonreduc ... | 1984 | 6707698 |
| human colostral cells. ii. response to mitogens. | the ability of colostral lymphocytes to respond to pokeweed mitogen, phytohemagglutinin, or epstein-barr virus was examined. none of these mitogens induced colostral cells to differentiate into immunoglobulin-containing cells, either in the absence or in the presence of mitomycin c-treated mononuclear cells or t-cell-enriched populations from peripheral blood. cocultivation of mononuclear cells from the peripheral blood of normal adults with mitomycin c-treated colostral cells resulted in a mark ... | 1984 | 6722892 |
| [polyradiculomeningoencephalitis caused by epstein-barr virus infection--description of a case with fatal outcome]. | a 21 years old high school student, who, since early childhood, suffered from complete spinal paraplegia of unknown cause at d 10, suddenly, after a three week period with influenca -like prodromal symptoms, presented with acute polyradiculomeningoencephalitis . positive reactions following paul-bunnell- davidsohn -test and tests for specific antivirus igg as well as specific igm revealed acute epstein-barr-virus infection, although no glandular signs could be found. the clinical findings consis ... | 1984 | 6724484 |
| malignant lymphoma, undifferentiated, burkitt's type in north-east italy. | burkitt's lymphoma occurred in 15 italian patients seen at pordenone general hospital over a period of seven years representing 8% of all malignant lymphomas seen in the same period. all patients were born and lived in a region of 100 miles radius around the north-eastern italian town of pordenone. five patients lived in the same rural village, and another two were distant relatives. there were eleven males and four females; the median age was 29 years (range 9-68) with ten patients older than 1 ... | 1984 | 6734005 |
| fulminant hepatic failure and fatal encephalopathy associated with epstein-barr virus infection. | a case of fulminant hepatic failure, associated with infectious mononucleosis, in a previously healthy 14-year-old girl is reported. despite intensive therapy, which included the use of acyclovir, charcoal haemoperfusion, and measures to control her raised intracranial pressure (guided by serial eeg and auditory brainstem-evoked response recordings), the outcome was fatal. | 1984 | 6738423 |
| sinusitis, orbital cellulitis and polymicrobial bacteremia in a patient with primary epstein-barr virus infection. | 1984 | 6739327 | |
| comparative results with various polyclonal b-cell activators in aneuploid chronic lymphocytic leukemia. | the mitotic index and number of abnormal metaphases of cells stimulated with the various b-cell polyclonal mitogens (pba) in six b cell chronic lymphocytic leukemia (b-cll) cases were evaluated. the pba included tetradecanoyl-0-phorbol-13-acetate (tpa), staphylococcus bacterial strain cowan i (cowan i), pokeweed mitogen (pwm), epstein-barr virus (ebv), and lipopolysaccharide w from e. coli 0.55:b5 (lps). tpa could stimulate only 1 of 12 samples. even though cowan i led to a relatively high mitot ... | 1984 | 6606485 |
| clinical significance of cytogenetic findings in untreated patients with b-cell chronic lymphocytic leukemia. | the chromosome constitutions of stimulated lymphocytes in 21 untreated cases with b-cell chronic lymphocytic leukemia (b-cll) were examined. so-called polyclonal b-cell activators, i.e., pokeweed mitogen, epstein-barr virus, and lipopolysaccharide w from e. coli 055:b5, were used. four out of 21 cases showed abnormal clones with trisomy 12 and were started on therapy shortly after the diagnosis and cytogenetic examination. on the other hand, most cases without abnormal clones had not received tr ... | 1984 | 6606486 |
| chronic myelogenous leukemia with a complex ph1 translocation in an xyy male. | we encountered a 38-year-old japanese male patient with chronic myelogenous leukemia (cml), whose bone marrow and peripheral blood cells during the chronic and blastic phases contained a complex ph1 translocation and an extra y chromosome [i.e., 47,xyy,t(9;22;13)(q34;q11;q14)]. a karyotypic analysis of pha-stimulated lymphocytes showed the constitutional karyotype to be 47,xyy. thus, it was considered that cml with a complex ph1 translocation developed in an xyy male; such a case has not been re ... | 1984 | 6607101 |
| xx t cells and xy b cells in two patients with severe combined immune deficiency. | immunologic evaluation of two unrelated male infants with clinical and laboratory evidence of severe combined immunodeficiency (scid) revealed t cells with a mature phenotype in the peripheral circulation of both patients although both had biopsy evidence of thymic alymphoplasia. both had a normal number of t cells with a cytotoxic/suppressor surface marker (okt8) but very few t helper cells (okt4). lymphocyte stimulation with the mitogens pha, con a, and pokeweed or with allogeneic cells result ... | 1984 | 6607807 |
| maternal transmission of acquired immune deficiency syndrome. | acquired immune deficiency syndrome (aids) has been reported in previously healthy homosexual or bisexual males, intravenous drug users, heterosexual men with hemophilia, and haitians. the finding of heterosexual hemophiliacs with aids has raised the possibility of a transmittable blood-borne agent as a cause of this disease. we have found three female half-siblings who had clinical and laboratory evidence for aids. all three had evidence of abnormal in vitro cellular immunity; two had chronic a ... | 1984 | 6608091 |
| cell cycle analysis of stimulated lymphocytes of b-cell chronic lymphocytic leukemia. | to clarify the cell cycle duration of stimulated cells in b cell chronic lymphocytic leukemia (b-cll), sister chromatid differentiation (scd) methodology was utilized. so-called polyclonal b-cell activators (pba), i.e., staphylococcus bacteria strain cowan i (cowan i), pokeweed mitogen (pwm), epstein-barr virus (ebv), and lipopolysaccharide w from e. coli 055:b5 (lps), were examined. most metaphases on day 2 (48 hr) of culture were in first division (m1), and about half of the metaphases on day ... | 1984 | 6608402 |
| folate metabolism in cells from fragile x syndrome patients and carriers. | the in vitro folate sensitivity of the fragile site at xq27 and the claims of a beneficial response of patients given folic acid prompted us to examine the folate metabolism in cells cultured from fragile x syndrome patients and carriers. using epstein-barr virus we established permanent lymphoblastoid lines from 4 fragile x syndrome males and 3 carriers from 7 families. all these lines expressed the fragile site when 0.1 microm 5-fluorodeoxyuridine (fudr) was added to the cultures 24 hr prior t ... | 1984 | 6585141 |
| distinct reactivity of burkitt's lymphoma cell lines with eight monoclonal antibodies correlated with the ethnic origin. | twenty-eight burkitt's lymphoma(s) (bl) cell lines were analyzed with anti-human immunoglobulins and monoclonal antibodies: y29/55, b1, and ba1 are slightly different pan-b-reagents; tu1 and bl13 are two discriminating markers of the follicle; rft1 is a pan-t-reagent expressed on the follicle mantle; al2 reacts with the common acute lymphoblastic leukemia antigen gp100; and 38:13 recognizes a bl-associated antigen. those lines were classified into 3 groups according to their membrane phenotype. ... | 1984 | 6592381 |
| a phorbol ester-binding inhibitory factor from human placenta. partial purification, characterization and biological effects. | a factor which inhibits the binding of 3h-phorbol-12,13-dibutyrate (pdbu) on different types of cells has been partially purified from human placenta. this factor, phorbol ester binding inhibitory factor (pebif), is sensitive to pepsin, but resistant to trypsin, heat and acid (ph 3) treatments and can be precipitated by 80% ethanol with no loss of activity. inhibition occurs both at 37 degrees c and 4 degrees c and is rapid and reversible. inhibition on human epithelial cells (fl), on mouse eryt ... | 1984 | 6598799 |
| opportunities for study of lymphoid neoplasia in africa. | animal models have strongly suggested that lymphoid leukaemias and malignant lymphomas might have a viral etiology. following the first report by ludwig gross in 1951 of the transmission of mouse leukaemia by cell-free filtrates inoculated into newborn mice and the subsequent identification of many tumour-inducing type-c viruses in animals, attention was focused on the rna retroviruses. however, it was work in africa which led to the recognition of burkitt's lymphoma as an entity and to the disc ... | 1984 | 6598801 |
| antibody to rheumatoid arthritis associated nuclear antigen (rana) in familial rheumatoid arthritis. | a search was made for the presence of antibody to the rheumatoid arthritis (ra) associated nuclear antigen (rana) in the sera of 57 affected and unaffected members of 10 families with multiple members affected with ra. while anti-rana was frequently observed among the seropositive affected individuals with definite or classical ra (60%), it was less frequently observed among those family members who had incompletely expressed disease, and rarely among normal close family members including those ... | 1984 | 6610751 |
| immortalized b lymphocytes produce b-cell growth factor. | the activation, clonal expansion and terminal differentiation of small resting b lymphocytes primed by an antigen (or antibodies to its receptors) appear to follow an orderly developmental sequence triggered at each stage by distinct soluble cytokines, primarily produced by t lymphocytes. for man, the only known b-cell mitogen independent of accessory cells for its action is the epstein-barr virus (ebv). lymphocytes transformed by ebv are released from the usual constraints on b-cell growth, pro ... | 1984 | 6610833 |
| [study of caryotypes in 52 cases of chronic lymphocytic b-cell leukemia]. | the karyotypes of 103 cases of b cell chronic lymphocytic leukemia were studied following stimulation or establishment of continuous cell lines using the epstein-barr virus. in 52 patients metaphases suitable for cytogenetic analysis were obtained; 30 revealed normal karyotypes and 22 abnormal karyotypes. the most frequently encountered abnormalities were 6 cases of extra chromosome 12, 4 cases of structured aberrations concerning chromosome 14, including 2 t(11;14), 4 translocations concerning ... | 1984 | 6610857 |
| polyclonal activation of b cells in homosexual men. | 1984 | 6611503 | |
| t-cell-mediated cytotoxicity against autologous malignant melanoma: analysis with interleukin 2-dependent t-cell cultures. | the cytotoxic reactivity of lymphocytes for autologous melanoma cells was studied in a group of 13 melanoma patients. no cytotoxicity was observed with lymphocytes freshly isolated from peripheral blood or with lymphocytes cocultured for 7 days with autologous melanoma cells. growth of lymphocytes previously sensitized with autologous melanoma in vitro in interleukin 2 (il-2)-containing medium, however, resulted in cytotoxic reactivity for autologous melanoma in 7/13 patients. the reactivity of ... | 1984 | 6610177 |
| unusual karyotypic changes and b cell involvement in a case of lymph node blast crisis of chronic myelogenous leukemia. | a patient with philadelphia chromosome (ph1) positive chronic myelogenous leukemia (cml) entered a blast crisis localized to lymph nodes. on light microscopy, by morphology and histochemical staining, the blasts were undifferentiated. in spite of terminal deoxynucleotidyl transferase positivity, some of the lymph node cells expressed a myeloid differentiation antigen, okm1, and were peroxidase positive by transmission electron microscopy (tem). however, the majority of cells were peroxidase nega ... | 1984 | 6610445 |
| immunoglobulin gene organization of ultraviolet-illuminated human lymphoblastoid cell lines producing both igm and igg. | we have studied the immunoglobulin gene organization of ultraviolet light (u.v.)-induced variant cells derived from an epstein-barr virus-transformed cell line. one variant produced igg1 and two variants produced both igm and igg1 whereas the parental cell line produced igm. southern blot analyses of dnas of these cells revealed a newly rearranged jh fragment in all the variants. the newly rearranged jh fragment also hybridized with the c gamma 1 sequence. the mu and gamma 1 chains produced in t ... | 1984 | 6443613 |
| brain mass as a manifestation of very late relapse in nonendemic burkitt's lymphoma. | a 4-year-old boy was diagnosed as suffering from burkitt's lymphoma with abdominal and maxillary involvement. the child was from a nonendemic area for burkitt's lymphoma, and no association with epstein-barr virus was found. two years after the completion of therapy, (3.25 years later) the child developed a solitary brain tumor, which was completely excised and was diagnosed as burkitt's lymphoma. no meningeal involvement was found. very late relapse in nonendemic burkitt's lymphoma is very rare ... | 1985 | 3967179 |
| comparison of five different methods of rubella igm antibody testing. | five tests for the detection of rubella specific igm antibody were compared. they were the conventional method of sucrose density gradient fractionation, followed by haemagglutination inhibition; an anti-mu capture radioimmunoassay; and three commercially available enzyme linked assays: rubazyme m, rubenz m i, and its successor, rubenz m ii. the five methods detected similar numbers of rubella positive samples between seven and 35 days after the onset of symptoms; in the earlier stages, however, ... | 1985 | 3968218 |
| epstein-barr virus infection and persistent fatigue. | 1985 | 3977208 | |
| time-dependent cytotoxic action of human recombinant alpha-interferon (ro22-8181) in vitro and the sensitivity of various cultured leukemia and lymphoma cell lines to it. | the growth inhibitory activity of human recombinant leucocyte a interferon (ro22-8181: alpha-interferon) against 23 human cultured cell lines derived from leukemias and lymphomas was measured quantitatively by regrowth assay. daudi cells were the most sensitive to it. two t-cell lines (rpmi-8402, hut78), three b-cell lines (raji, ly16, a3/kawakami), one non-t, non-b acute lymphoblastic leukemia (all) cell line (kopn-1) and three myelomonocytoid cell lines (u937, thp-1, ml-1) were moderately or s ... | 1985 | 3981816 |
| increased cytosolic calcium in cystic fibrosis neutrophils effect on stimulus-secretion coupling. | a disorder of calcium homeostasis has been related to the pathogenesis of cystic fibrosis (cf). the authors have studied the relationship between the cytosolic free calcium concentration ([ca2+]i), the amount of ca2+ released from endogenous stores and the secretory response in cf neutrophils. significantly elevated resting [ca2+]i and depressed ca2+ release induced by the chemotactic peptide n-formyl-l-methionyl-l-leucyl-l-phenylalanine (fmlp) is present in cf neutrophils. in the absence of exo ... | 1985 | 3982226 |
| rare complications and unusual syndromes associated with epstein-barr virus. | 1985 | 3982987 | |
| life-threatening haemolytic anaemia and infectious mononucleosis. a case report. | a 36-year-old man with infectious mononucleosis presented with massive intravascular haemolytic anaemia. the diagnosis was established by a positive paul-bunnell test, the demonstration of igm antibodies against epstein-barr virus capsid, and igg antibody to early antigen. the direct and indirect antiglobulin tests were negative; the pathogenesis of the severe haemolysis was attributed to the temporary synthesis of anti-i cold agglutinin having a high thermal amplitude. although life-threatening ... | 1985 | 3983760 |
| low-density lipoprotein receptor deficiency resulting in familial hypercholesterolaemia in a black man. a follow-up study. | the binding and catabolism of low-density lipoprotein (ldl) was measured in cultured skin fibroblasts and epstein-barr virus-transformed lymphoblastoid cells from a south african black man. on the basis of ldl receptor activity, the patient was classified as a familial hypercholesterolaemic homozygote of the ldl receptor-defective type. | 1985 | 3983774 |
| reactivation of chronic type b hepatitis presenting as acute viral hepatitis. | three asymptomatic chronic carriers of hepatitis b surface antigen, who had normal serum aminotransferase levels and no detectable hepatitis b e antigen in serum, developed icteric, symptomatic acute hepatitis. serologic evidence of acute infection with hepatitis a virus, delta hepatitis virus, cytomegalovirus, or epstein-barr virus was absent. however, hepatitis b virus dna and dna polymerase activity, which were not detectable before the exacerbation, appeared in the serum of all three patient ... | 1985 | 3994187 |
| lymphocytic interstitial pneumonia associated with the acquired immune deficiency syndrome. | lymphocytic interstitial pneumonitis (lip) is characterized by interstitial accumulation of mature lymphocytes, plasma cells, and reticuloendothelial cells and is often an unremitting process unresponsive to immunosuppressive therapy. the patient described in this report had severe candidal esophagitis and immunologic findings consistent with the acquired immune deficiency syndrome (aids). there was no evidence of pulmonary infection with pneumocystis carinii, cytomegalovirus, mycobacterium aviu ... | 1985 | 4003948 |
| the isolation and characterization of the blym-1 transforming gene. | there are a number of similarities between chicken bursal lymphomas and human burkitt's lymphomas. both lymphomas are associated with viral infection, by llv in bursal lymphomas and epstein-barr virus in burkitt's lymphoma. avian lymphoid leukosis virus integration is associated with enhanced c-myc expression, while the role ebv plays in tumorigenesis remains unclear. in burkitt's lymphoma, however, c-myc activation does occur as a result of specific chromosomal translocations involving the huma ... | 1985 | 4015580 |
| phenotypic analysis of an established cell line derived from a patient with hodgkin's disease (hd). | this report describes the phenotypic analysis of a cell line obtained from a female patient with the nodular sclerosing subtype of hodgkin's disease (hd). the cell line has a neoplastic karyotype and is stable in culture in the absence of feeder layers or growth factors. phenotypic analysis of this cell line shows that it cannot easily be characterized as either a lymphocyte, macrophage or granulocyte but resembles in its characteristics certain hd lines already described in the literature. the ... | 1985 | 4018743 |
| endemic african kaposi's sarcoma is not associated with immunodeficiency. | twenty-seven histologically confirmed kaposi sarcoma (ks) patients resident in the kivu lake area of eastern zaire were examined for immune competence. only ks cases of the endemic african type have been observed in this high-incidence area. the median duration of the symptoms was 6 years and ranged from 1 to 38 years. forty-one controls matched for age, sex and tribe and unrelated to the ks patients were selected from the community. thirteen additional controls were first-degree relatives of th ... | 1985 | 4018905 |
| properties of succinic semialdehyde dehydrogenase in cultured human lymphoblasts. | a direct assay has been developed for succinic semialdehyde dehydrogenase in sonicates of human lymphocytes and epstein-barr virus transformed cultured lymphoblasts. enzyme activity was quantified by incubating cell extracts with uniformly labeled [14c]succinic semialdehyde and monitoring the conversion to [14c]succinic acid. radiolabeled products were separated by liquid partition chromatography on hydrated silicic acid. kinetic properties and requirements of succinic semialdehyde dehydrogenase ... | 1985 | 4020531 |
| viral antibodies in twins with multiple sclerosis. | viral antibodies to measles, rubella, corona, vaccinia, and mumps viruses in serum and csf (and to epstein-barr virus in serum only) were studied in 24 twin pairs, both discordant and concordant for clinical ms. in pairs, csf antibody titers for rubella in ms monozygotic and dizygotic twins and for vaccinia in dizygotic twins were higher than for unaffected twins. increased csf titers among ms twins existed for measles, rubella, and vaccinia when pairing was ignored. among ms twins, serum rubell ... | 1985 | 4040615 |
| sporadic postinfectious neuromyasthenia. | outbreaks of epidemic neuromyasthenia have occurred throughout the world for many years, but sporadic cases have only recently been recognized. fifty consecutive previously well patients with prolonged and excessive fatigue after an apparent acute infection were investigated. most were well educated, active, unmarried women aged 30 to 40 years. the precipitating infection had many clinical presentations. the chronic phase of the illness was characterized by a fairly common set of symptoms. physi ... | 1985 | 4042036 |
| a human monoclonal igg1 lambda anti-hepatitis b surface antibody. production, properties, and applications. | peripheral blood mononuclear cells from a donor with a high titre of anti-hepatitis b surface (hbs) antibodies were fused with a cell line that was positive for epstein-barr virus nuclear antigen and sensitive to hypoxanthine-aminopterine-thymidine. a cell line was established that produces a monoclonal igg1 lambda anti-hbs antibody. afterwards, it appeared that the anti-hbs antibody-producing cell line had arisen from epstein-barr virus transformation of the donor b cells. the cell line is capa ... | 1985 | 4048870 |
| prospective studies on nasopharyngeal carcinoma in epstein-barr virus iga/vca antibody-positive persons in wuzhou city, china. | a serological mass survey was carried out in wuzhou city in 1980, 1,136 iga/vca-positive persons being followed up for 4 years. altogether 35 npc cases were detected, of which 15 (43%) were in stage i and 17 (48.5%) in stage ii, early cases (i + ii) thus amounting to 91.5%. the detection rate of early cases was 2.9 times higher than in our outpatient clinic. iga/vca antibody could be detected 16-41 months prior to clinical diagnosis of npc. we conclude that, if iga/vca-positive individuals are e ... | 1985 | 4055129 |
| the identification of n-linked oligosaccharides on the human cr2/epstein-barr virus receptor and their function in receptor metabolism, plasma membrane expression, and ligand binding. | human complement receptor type 2 (cr2) was biosynthetically labeled by pulsing sb b lymphoblastoid cells for 25 min with [35s]methionine followed by chase in the presence of excess unlabeled methionine. an mr 134,000 polypeptide represented the major form of the receptor at the end of the pulse period, and within 1 h of chase this disappeared coincident with the appearance of the mr 145,000 mature form of cr2. precursor, but not mature, cr2 was sensitive to endoglycosidase h, indicating that mat ... | 1985 | 4055758 |
| diphtheria toxin resistance in human lymphocytes and lymphoblasts in the in vivo somatic cell mutation test. | it has been shown that circulating peripheral blood lymphocytes can be used for the enumeration of 6-thioguanine-resistant cells that presumably arise by mutation in vivo. this somatic cell mutation test has been studied in lymphocytes from human populations exposed to known mutagens and/or carcinogens. the sensitivity of the test could be further enhanced by including other gene markers, since there is evidence for locus-specific differences in response to mutagens. resistance to diphtheria tox ... | 1985 | 4065058 |
| burkitt's lymphoma in japan. | the clinicopathological features of 18 japanese patients with burkitt's lymphoma are reviewed. six patients were 12 years of age or younger. seven patients presented with abdominal masses and seven with jaw tumours. thirteen patients manifested blood and/or marrow involvement at the time of initial diagnosis (five patients) or later in the course of the disease (eight patients). most of the patients had a rapid clinical course in spite of combination chemotherapy. the malignant cells from all 17 ... | 1985 | 4065943 |
| 38.13: a monoclonal antibody directed against a burkitt's lymphoma-associated antigen and its use as carrier for toxins. | we have previously described a monoclonal antibody, referred to as 38.13, reacting with most burkitt's lymphoma (bl) derived lines, both with and without epstein-barr virus (ebv), but not reacting with ebv-positive lymphoblastoid cell lines (wiels et al., 1981, 1982). the antibody reacted with 41 bl lines out of 57 tested. the target antigen, bla, was shown to be a neutral glycolipid, identified as globotriaosylceramide (gal alpha 1----4gal beta 1----4 glc beta 1----1 ceramide) (nudelman et al., ... | 1985 | 4065950 |
| gianotti-crosti syndrome in association with epstein-barr virus altered by ampicillin. | 1985 | 4072847 | |
| insulin receptor biosynthesis in cultured lymphocytes from insulin-resistant patients. | in some patients with genetic forms of extreme insulin resistance, the cause of insulin resistance is a marked (greater than or equal to 90%) reduction in the number of insulin receptors on the cell surface. in the present work, we describe studies of insulin receptor biosynthesis in epstein-barr virus (ebv)-transformed lymphocytes from three patients (a-1, a-5, and a-8) with type a extreme insulin resistance. insulin receptors are composed of two major glycoprotein subunits (apparent molecular ... | 1985 | 4077982 |
| characterisation of a new cell line (cj18) from a patient with 'hairy' cell leukaemia. | a cell line (cj18) has been established from the peripheral blood of a patient with hairy cell leukaemia (hcl) in a leukaemic phase. they grow slowly in large clumps with a doubling time of 3-4 d. 8% show positivity for surface immunoglobulin g and a small percentage (5%) are positive for intracytoplasmic immunoglobulin. they are b1,ia1 positive and call, tdt and okm1 negative. although they are epstein barr virus nuclear antigen (ebna) positive they have several features not found in other ebna ... | 1985 | 4081642 |
| [hematologic complications in infectious mononucleosis]. | in three patients with infectious mononucleosis the disease was severely complicated by, respectively, aplastic anemia, consumptive thrombocytopenia and hemolytic anemia with acute renal failure. in contrast to the regularly recurring basic signs and symptoms of the illness, serious complications occur in less than 1% of all patients. changes in the immunologic system seem to play an important role in hematologic and other complications, as well as in the occasionally fatal epstein-barr virus in ... | 1985 | 4089583 |
| [pediatric papular acrodermatitis and double primary infection by the hepatitis b virus and the epstein-barr virus]. | a case of papular infantile acrodermatitis was evaluated in a twenty-two month-old child. laboratory data showed the presence of a cytolytic hepatitis associated to an increase in circulating monocytes (1.500/mm3) with hyperbasophilic cells. hepatitis b surface (hbs) antigen was detected in the serum, associated to anti-hbc antibodies of the igm class, without detectable anti-hbs antibodies. simultaneously, ebv serologic profiles were consistent with a primary infection. the authors review the c ... | 1985 | 4091411 |
| spontaneous rupture of the spleen due to acquired immunodeficiency syndrome in an intravenous drug abuser. | a case of spontaneous rupture of the spleen is described in a 25-year-old woman with a history of intravenous drug abuse. at autopsy the spleen and the lymph nodes were soft, slightly enlarged, and markedly congested. they showed reactive follicular hyperplasia with increased cellularity and prominent vascularity of the red pulp and the interfollicular tissue. the postmortem serum was positive for antibodies to human t-cell lymphotropic virus type iii. other serologic tests were negative, includ ... | 1985 | 3840986 |
| a new myelomonoblastic cell line (m20): analysis of properties, differentiation, and comparison with other established lines of similar origin. | a new myelomonoblastic cell line (m20) was established from the peripheral blood of a ten-year-old child with acute myeloblastic leukemia, using an improved method for supporting the initial stages of cell proliferation. the addition of irradiated macrophage monolayers to the proliferating cells appeared to overcome the deterioration of the primary cultures and enable them to continue proliferating until they became independent of this environment. the cell line that developed consisted of myelo ... | 1985 | 3857183 |
| immunologic markers of burkitt's lymphoma cells. | lymphocyte markers were studied on fresh cells from 30 patients with burkitt (l3) leukaemia and cell lines derived from endemic and non-endemic burkitt's lymphoma (bl) patients. we observed day-to-day variations of lymphocyte marker expression by cultured lines and, occasionally, differences between fresh and cultured cells. in l3 leukaemia, a wide range of phenotypes, including pre-b cell and mature monoclonal igm + igd positive b-cell phenotypes, was observed. most often, the cells expressed h ... | 1985 | 3864747 |
| virus-associated haemophagocytic syndrome after epstein-barr virus infection in a child. | 1985 | 3866003 | |
| the clinical pharmacology of acyclovir and its prodrugs. | acyclovir (zovirax) is a highly specific antiherpes virus agent. extensive investigations of the pharmacokinetics in man have shown it to have a useful half-life of about three hours and to be largely excreted unchanged in the urine. crystaluria can be avoided provided the patient is well hydrated and attention is paid to the dosing instructions especially in patients with renal failure. in vitro ed50s (the drug concentration inhibiting virus replication by 50%) bear some general relevance to ef ... | 1985 | 3868024 |
| comparative structural analysis of hla-a3 antigens distinguishable by cytotoxic t lymphocytes: variant e1. | influenza-specific cytotoxic t cells restricted by hla-a3 recognize differences between hla-a3 antigens that are serologically indistinguishable. to examine whether this differential recognition had a structural basis, we have compared the structures of hla-a3 molecules from epstein barr virus-transformed peripheral blood lymphocytes of two individuals, e1 and m17. m17 was representative of the majority of hla-a3-bearing individuals, whereas e1 was a variant distinguished by cytotoxic t cells. p ... | 1985 | 3871112 |
| identification of an additional class of c3-binding membrane proteins of human peripheral blood leukocytes and cell lines. | proteins binding the third component of complement (c3) were isolated by affinity chromatography from surface-labeled solubilized membranes of human peripheral blood cells and cell lines. the isolated molecules were subjected to nadodso4/page, and autoradiographs of these gels indicated that c3-binding proteins could be divided into three groups based on mr: (i) gp200, an approximately 200,000 mr molecule previously identified as the c3b/c4b receptor or cr1; (ii) gp140, an approximately 140,000 ... | 1985 | 3871945 |
| in vitro expansion and analysis of cloned cytotoxic t cells derived from patients with chronic t gamma lymphoproliferative disorders. | patients with t gamma lymphocytosis are a heterogeneous group, clearly distinguishable from other patients with chronic t lymphoproliferative disorders and usually without proven malignancy. we have attempted in vitro cloning of lymphocytes from three patients with an expansion of phenotypically and functionally different types of t gamma cells. one had t3+ b73.1+ t4 t8+ okm1+ t gamma cells exerting antibody-dependent cellular cytotoxicity (adcc) and natural killer (nk) cell cytotoxicity; anothe ... | 1985 | 3872139 |
| accessory function of human leukemic cell lines: properties of b and b-k562 hybrid cell lines. | the accessory function (af) of various b cell lines has been investigated by studying their ability to replace monocytes inducing the proliferation of highly purified t lymphocytes in the presence of phytohemagglutinin. af could be exerted by b cells from different origin, including epstein-barr virus (ebv) transformed lymphoblastoid cell lines (lcl), burkitt's lymphoma (bl)-derived lines, either ebv-positive or not, pre-b and b leukemias and lymphomas. the ebv-converted bjab-b95 bl cells could ... | 1985 | 3872216 |
| fmc-hu-1-b: a lymphoma b-cell line with unusual characteristics. | a cell line (fmc-hu-1-b) was established from a biopsy of an abdominal mass of a child with non-burkitt's lymphoma. the establishment of the cell line initially required the presence of normal bone marrow stromal cells and phytohaemagglutinin stimulated leucocyte conditioned medium. the cell line lacked epstein-barr virus nuclear antigen and exhibited numerous chromosomal abnormalities. cell-surface marker analysis using a panel of monoclonal antibodies revealed only markers of the b lineage. wi ... | 1985 | 3872973 |
| isoenzyme studies in human leukemia-lymphoma cells lines--ii. acid phosphatase. | this report describes the qualitative acid phosphatase (acp) isoenzyme profiles detected in permanent human hematopoietic cell lines. the acp activity was separated into its isoenzymes by isoelectric focusing on horizontal thin-layer polyacrylamide gels. the pattern of acp isoenzyme was investigated in a total of 86 cell lines. these cell lines were classified into five groups on the basis of their phenotypes characterized in the multiple marker analysis: 74 leukemia-lymphoma cell lines (26 t-, ... | 1985 | 3874327 |
| heterogeneity of b cell involvement in acute nonlymphocytic leukemia. | in order to study the pattern of b cell involvement in acute nonlymphocytic leukemia (anll), multiple b lymphoid cell lines were established by epstein-barr virus transformation of peripheral blood mononuclear cells from two patients with the disease who were heterozygous for the x chromosome-linked glucose-6-phosphate dehydrogenase (g6pd). in one patient, the progenitor cells involved by the leukemia exhibited multipotent differentiative expression, whereas in the other patient the cells showed ... | 1985 | 3874662 |
| malignant transformation of bloom syndrome b-lymphoblastoid cell lines by carcinogens. | three types of bloom syndrome b-lymphoblastoid cell lines, as well as one derived from a normal person, treated with 4-nitroquinoline-n-oxide and n-methyl-n'-nitro-n-nitrosoguanidine (0.3 micrograms/ml for 24 hr), were studied for tumorigenicity in nude mice, colony formation in soft agar, cytogenetic changes, and immunoglobulin markers. when normal and bloom syndrome cells with normal sister chromatid exchange (sce) levels and karyotypes (type i) were treated with carcinogens, no significant ch ... | 1985 | 3875094 |
| the expression of the hodgkin's disease associated antigen ki-1 in reactive and neoplastic lymphoid tissue: evidence that reed-sternberg cells and histiocytic malignancies are derived from activated lymphoid cells. | ki-1 is a monoclonal antibody (raised against a hodgkin's disease-derived cell line) that, in biopsy tissue affected by hodgkin's disease, reacts selectively with reed-sternberg cells. the expression of ki-1 antigen has been analyzed by immunocytochemical techniques in a wide range of human tissue and cell samples, including fetal tissue, malignant lymphomas (290 cases), and mitogen- and virus-transformed peripheral blood lymphocytes. the antigen was detectable on a variable proportion of cells ... | 1985 | 3876124 |
| elevated allogeneic cytotoxic t lymphocyte activity in peripheral blood leukocytes of homosexual men. | peripheral blood leukocytes (pbl) from male homosexual and heterosexual volunteers who did not have evidence of acquired immunodeficiency syndrome (aids) were studied for their ability to generate cytotoxic t lymphocyte (ctl) responses in vitro to allogeneic stimulator pbl from a single individual or from a pool of donors (allo-pool). seventeen of 39 homosexual donors generated strong primary ctl activity to a single randomly selected stimulator donor, whereas only two of 16 heterosexual donors ... | 1985 | 3876378 |
| immunoglobulin synthesis in a patient with c4 deficiency: functional immaturity of peripheral blood lymphocytes. | peripheral blood lymphocytes from a systemic lupus erythematosus patient with homozygous c4 deficiency were stimulated in vitro. although t cell independent stimulation (epstein-barr virus) induced normal proportions of igm secreting cells. igg and iga secreting cells were almost absent. virtually no immunoglobulin secretion was obtained after t cell dependent stimulation (pokeweed mitogen). cell fractionation experiments indicated that the low pokeweed mitogen response, at least with regard to ... | 1985 | 3876686 |
| histogenesis of burkitt's lymphoma: a b-cell tumour of mucosa-associated lymphoid tissue. | the designation burkitt's lymphoma (bl) has proved to be of great convenience but has left undetermined the histogenesis of the tumour and its relationship to other b-cell lymphomas. one definition proposed for bl has been based on its relationship to epstein-barr virus (ebv). the ebv negativity of some bls and the association of ebv with other non-hodgkin's lymphoma, however, makes this definition unsatisfactory. cytogenetic changes involving the translocations of part of chromosome 8 also are ... | 1985 | 3877685 |
| b lymphocyte regulation of human hematopoiesis. | epstein barr virus (ebv)-transformed b lymphoblastoid cell lines (blcl) were derived from seven different individuals. the ability of blcl supernatants to stimulate hematopoietic colony formation in vitro was tested in a conventional stem cell assay system. supernatants promoted the growth of single (gm, e, mk) as well as multi-lineage (gemm) colonies in bone marrow cultures. our results indicate that ebv-transformed b lymphocytes produce cytokines that affect in vitro stem cell proliferation an ... | 1985 | 3877756 |
| defective specific antiinfluenza virus antibody production in vitro by lymphocytes from patients with ataxia-telangiectasia. | the ability of lymphocytes from 11 patients with ataxia-telangiectasia to produce specific antiinfluenza virus antibody in vitro was evaluated. lymphocytes from these patients produced markedly less antibody than lymphocytes from normal controls when stimulated with type a influenza viruses. additional studies were undertaken to evaluate the function of the b cells, t cells, and adherent cells of these patients in specific antibody production. b cells from the at patients produced one-third to o ... | 1985 | 3877788 |
| ligands of surface ig raise cytoplasmic free ca++ in human b cells. | with the use of the fluorescent ca++ indicator quin-2, we have measured changes in intracellular calcium levels in human b cells in response to anti-ig antibodies, to staphylococcus aureus (staph) or to protein a. cells of an epstein-barr virus-transformed mu lambda-carrying b-cell line, azu-1, increased free cytosolic calcium after addition of anti-mu or anti-lambda antibodies; f (ab')2 fragments with anti-mu specificity were equally effective. fab fragments of sheep anti-ig antibodies only ind ... | 1985 | 3878580 |
| heterogeneity of immune defects in three children with a chronic active epstein-barr virus infection. | three children, all girls, showed long-lasting clinical and serologic evidence of chronic active epstein-barr virus (ebv) infection. extremely high serum titers of igg- and iga-type vca antibodies and ea antibodies were present, whereas ebna antibody titers were in the range of those found in seropositive individuals. all three patients repeatedly showed the presence of nonspecific pokeweed mitogen (pwm)-activatable suppressor cells in the peripheral blood. the analysis of ebv-specific cytotoxic ... | 1985 | 3878848 |
| [persistent epstein-barr virus infection]. | 2 boys aged 4 and 6 1/2 years and a 1 1/2-year-old girl in whom persistent ebv-infections developed are described. serological investigations showed a markedly increased igg-antibody titer against the virus-capsid antigen (vca-igg). furthermore there was a persistence of anti-early-antigen-antibodies (anti-ea-antibodies) resp. vca-iga indicating a chronic infection. the observation period was 1 to 4 years. cellular immunity in these children was depressed. they show the typical clinical symptoms ... | 1985 | 3878942 |
| morphologic, immunologic, enzymehistochemical and chromosomal analysis of a cell line derived from hodgkin's disease. evidence for a b-cell origin of sternberg-reed cells. | cell lines derived from hodgkin's disease may provide a clue to the nature of sternberg-reed cells. in the current study, the establishment of an epstein-barr-virus-negative lymphoblastoid cell line, derived from the pleural fluid of a patient with the nodular sclerosis type of hodgkin's disease, is described. the morphologic and immunologic cell marker findings indicate that this cell line is derived from sternberg-reed cells. the immunologic findings and a chromosomal analysis are in agreement ... | 1985 | 3881158 |
| immunoglobulin m and immunoglobulin g secretion by human b cells exposed to ru 41.740, a glycoprotein extract from klebsiella pneumoniae. | ru 41.740, a glycoprotein extract from klebsiella pneumoniae, was seen to activate human b cells to immunoglobulin secretion in vitro. the effects of ru 41.740 on human b cells were compared to those induced by pokeweed mitogen, a t-cell-dependent polyclonal b-cell activator, and epstein-barr virus, a t-cell-independent polyclonal b-cell activator. exposure of human b cells to all of these agents resulted in increased immunoglobulin m (igm) and immunoglobulin g (igg) secretion. igm and igg secre ... | 1985 | 3881193 |
| antibodies to rheumatoid arthritis nuclear antigen (rana) in japanese patients with rheumatoid arthritis. | we studied antibodies to rheumatoid arthritis nuclear antigen (rana) by the ouchterlony method in 0.5% agarose plates, using soluble antigen extracted with 0.25 m sucrose solution from cultured raji cells. anti-rana antibody was found in sera from 24 to 40 (60%) patients with rheumatoid arthritis (ra), from 4 of 20 (20%) patients with systemic lupus erythematosus (sle), and from 2 of 30 (7%) healthy controls. when sucrose extracts from bjab, ramos, and jm cells were used as the cellular antigens ... | 1985 | 3885372 |
| leukocyte lfa-1, okm1, p150,95 deficiency syndrome: functional and biosynthetic studies of three kindreds. | three patients (2 female, 1 male) with recurrent infection, granulocytosis, impaired pus formation, and/or delayed umbilical cord separation were identified. assessments of polymorphonuclear leukocytes (pmn)/monocyte function in each patient revealed profound abnormalities of adherence and adherence-dependent functions. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) of their pmn lysates demonstrated a deficient or absent protein(s) of 138 kilodaltons (gp 138). na3hb4 labeli ... | 1985 | 3891420 |
| a monoclonal antibody to human transitional-cell carcinoma of the bladder cross-reacting with a differentiation antigen of neutrophilic lineage. | a monoclonal antibody (mab) to human transitional-cell carcinoma of the bladder (tccb) was obtained by immunization of a balb/c mouse with formalin-fixed tccb cells and subsequent fusion of the spleen cells with sp2-oag14 myeloma line. gf 26.7.3 mab was selected by indirect immunofluorescence (iif) as reacting agent with target cells and negative with autologous lymphocytes and epstein-barr virus (ebv)-transformed lymphoblastoid cell-line. gf 26.7.3 reacts with a high percentage of bladder and c ... | 1985 | 3891639 |
| nucleic acid hybridization in the diagnosis of viral infections. | recombinant dna technology, including molecular cloning and nucleic acid hybridization, is now being applied to problems in clinical virology. although viral isolation in cell culture remains the most sensitive and specific diagnostic test for many viruses, for some viruses, isolation in cell culture is lengthy or difficult or has not yet been achieved. utilization of hybridization techniques has already resulted in important new information concerning the pathogenesis of a number of viruses, su ... | 1985 | 3899479 |
| epidemiological evidence for the role of falciparum malaria in the pathogenesis of burkitt's lymphoma. | nearly all epidemiological characteristics of burkitt's lymphoma (bl) can be explained on the basis of relationships of bl to the intensity of the host response to plasmodium falciparum. the major epidemiological associations are: the high degree of geographic correlation between the incidence rate of bl and the intensity of p. falciparum transmission, both at a global level and within individual countries; the close correlation between the age incidence of bl and the age of acquiring maximum le ... | 1985 | 3905588 |
| indication for bone-marrow harvesting and purging in burkitt's lymphoma: a three-year experience. | between 1980 and 1983, 317 bone-marrow (bm) aspirates from 63 cases of burkitt's lymphoma (bl) were studied with an in-vitro liquid culture monitoring system. bl cell lines were obtained in culture from 14 of 15 patients with cytologically positive marrow. the in-vitro monitoring system was shown to be useful regardless of epstein-barr virus (ebv) status, clinical status of patients and cytogenetic anomalies (8 t(8;14) 2 t(8;22) 2 when bm was cytologically normal or suspect, i.e., less than 5% b ... | 1985 | 3905595 |
| three distinct antigen systems on human b cell subpopulations as defined by monoclonal antibodies. | three novel antigen systems (l22, l23, and l24) expressed on human b cell subpopulations were identified by using tb1-2c3, tb1-2b3, and tb1-3c1 monoclonal antibodies, respectively. l22 was expressed on a minor subpopulation of b cells in human lymphoid tissues and in the peripheral blood. these b cells associated with l22 were resting small b cells mainly located in the mantle zone of lymphoid follicles, most of which also expressed igm and igd on their cell membrane. this antigen was absent fro ... | 1985 | 3917477 |
| b cells in patients with x-linked agammaglobulinemia. | x-linked agammaglobulinemia (xla) has been described as a disorder in which pre-b cells fail to differentiate into b cells. however, a small number of b cells have been seen occasionally in patients with this disorder. because the phenotype of these cells might be helpful in defining the site of the defect in xla, immunofluorescent staining techniques were used to characterize the b cells that can be found in patients with xla. surface igm-positive b cells could be detected in the peripheral cir ... | 1985 | 3920309 |
| absence of detectable hepatitis b virus dna in sera and liver of chimpanzees with non-a, non-b hepatitis. | the risk of hepatitis b infections has been reduced by screening of blood donors for hepatitis b surface antigen (hbsag). however, recipients remain at significant risk of developing post-transfusion hepatitis. studies have shown that non-a, non-b hepatitis virus(es) are responsible for the majority of post-transfusion hepatitis infections. in spite of many efforts, these non-a, non-b hepatitis viruses have not yet been identified. epidemiological studies, however, suggest that non-a, non-b hepa ... | 1985 | 3920354 |
| non-a, non-b hepatitis associated with blood transfusion. | posttransfusion hepatitis of the non-a, non-b variety continues to be a significant problem in current hemotherapy. this disorder is many times more common than transfusion-associated disease caused by hepatitis b virus, cytomegalovirus, and epstein-barr virus, and also seems to be viral in origin. several potential etiological agents have been implicated, but none has been identified with certainty, despite concerted efforts at doing so. clinical disease is usually attended by few symptoms and ... | 1985 | 3920792 |
| two different t-cell systems in humans, one of which is probably equivalent to qa or tla in mice. | two different alloantigenic systems, expressed mainly on tg and tm lymphocytes and called tca and tcb, respectively, are described. alloantisera from parous women are absorbed with epstein-barr virus-transformed b cell lines from the husband of the serum producer to remove all anti hla-a,b,c and dr antibodies. the absorbed sera are tested against a random panel and against lymphocytes from families. family studies indicate that the tca system might be encoded by a gene linked to hla and located ... | 1985 | 3921498 |
| evidence for the involvement of b lymphoid cells in polycythemia vera and essential thrombocythemia. | previous studies with the x-chromosome-linked glucose-6-phosphate dehydrogenase (g6pd) as a marker of cellular mosaicism demonstrated that polycythemia vera (pv) and essential thrombocythemia (et) are clonal disorders of hematopoietic stem cells that can differentiate to erythrocytes, granulocytes, and platelets. to determine if the involved stem cells could also differentiate along the b-lymphoid pathway, we studied one woman with pv and one woman with et. of 117 epstein-barr virus-transformed ... | 1985 | 3921571 |