Publications

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development of an in vivo model for toxoplasma gondii infections in chickens and turkeys simulating natural routes of infection.turkeys and chickens were orally infected with tissue cysts (one mouse brain) or oocysts (103, 105 or 106 oocysts) of three t. gondii strains of the clonal types ii and iii (me49, cz-tiger, ned) to investigate the influence of the applied t. gondii strain and infective doses on the distribution of t. gondii in several organs and tissues and the serologic response of chickens and turkeys. organ samples from 16 different tissues, including heart, brain, muscles and gizzard were analyzed by pcr. br ...201931706235
evaluating responses to temperature during pre-metamorphosis and carry-over effects at post-metamorphosis in the wood tiger moth (arctia plantaginis).insect metamorphosis is one of the most recognized processes delimiting transitions between phenotypes. it has been traditionally postulated as an adaptive process decoupling traits between life stages, allowing evolutionary independence of pre- and post-metamorphic phenotypes. however, the degree of autonomy between these life stages varies depending on the species and has not been studied in detail over multiple traits simultaneously. here, we reared full-sib larvae of the warningly coloured w ...201931438813
towards the tiger international framework for recommendations of core competencies in health informatics 2.0: extending the scope and the roles.this paper describes the methodology and developments towards the tiger international recommendation framework of core competencies in health informatics 2.0. this framework is meant to augment the scope from nursing towards a series of six other professional roles, i.e. direct patient care, health information management, executives, chief information officers, engineers and health it specialists and researchers and educators. health informatics core competency areas were compiled from various s ...201931438119
eye-of-the-tiger sign in neurodegeneration with brain iron accumulation.a 68-year-old male patient presented to the neurology clinic with tremor, lightheadedness, and a history of syncope. exam showed mild parkinsonism. neuroimaging revealed symmetric lesions of the globus pallidus (the eye-of-the-tiger sign) concerning for neurodegeneration with brain iron accumulation (nbia). genetic panel for nbia was ordered, specifically pantothenate kinase-associated neurodegeneration (pkan), including pantothenate kinase 2 (pank2) - the genetic marker for the pantothenate kin ...201931431841
modeling the inactivation of bacillus cereus in tiger nut milk treated with cold atmospheric pressure plasma.the impact of cold atmospheric pressure plasma treatment on the inactivation kinetics of bacillus cereus atcc 14579 and the resulting quality changes was investigated in tiger nut (cyperus esculentus l.) milk (tnm). the effect of input power (39, 43, and 46 w) and treatment time (0 to 270 s) was fitted using the weibull model to represent the microbial kinetic inactivation in the treated tnm. inactivation efficacy increased with an increase in treatment time and input power. a 5.28-log reduction ...201931596617
impact of multiple food environments on body mass index.although the relationship between residential food environments and health outcomes have been extensively studied, the relationship between body mass index (bmi) and multiple food environments have not been fully explored. we examined the relationship between characteristics of three distinct food environments and bmi among elementary school employees in the metropolitan area of new orleans, la. we assessed the food environments around the residential and worksite neighborhoods and the commuting ...201931390365
identification and expression profile of delta-gst in black tiger shrimp (penaeus monodon) exposed to aflatoxin b1 (afb1).delta gst is an insect-specific class and a prominent class of the glutathione s-transferases family that is involved in xenobiotic detoxification and antioxidant defense. the full-length complementary dna of delta-class gst from penaeus monodon (pmdeltagst; 839 bp long with a 657 bp coding region) was cloned. the encoded polypeptide of 218 amino acids had a predicted molecular mass of 24.30 kda. sequence homology and phylogenetic analysis showed that pmdeltagst was significant similarity to gst ...201931389197
clade 2.3.2.1 h5n1 avian influenza viruses circulate at the interface of migratory and domestic birds around qinghai lake in china.during 2012-2015, six h5n1 avian influenza viruses were isolated from domestic birds and the environment around qinghai lake. phylogenetic analysis of ha genes revealed that a/chicken/gansu/xg2/2012 (ck/gs/xg2/12) belonged to clade 2.3.2.1a, while a/environment/qinghai/1/2013 (en/qh/1/13), a/chicken/qinghai/qh1/2015 (ck/qh/qh1/15), a/chicken/qinghai/qh2/2015 (ck/qh/qh2/15), a/chicken/qinghai/qh3/2015 (ck/qh/qh3/15), and a/goose/qinghai/qh6/2015 (gs/qh/qh6/15) belonged to clade 2.3.2.1c. further ...201931383307
the insect apocalypse, and why it matters.the majority of conservation efforts and public attention are focused on large, charismatic mammals and birds such as tigers, pandas and penguins, yet the bulk of animal life, whether measured by biomass, numerical abundance or numbers of species, consists of invertebrates such as insects. arguably, these innumerable little creatures are far more important for the functioning of ecosystems than their furry or feathered brethren, but until recently we had few long-term data on their population tr ...201931593678
tetrodotoxin functions as a stress relieving substance in juvenile tiger puffer takifugu rubripes.we tested whether tetrodotoxin (ttx) functions as a stress relieving substance in puffer fish. we orally administered ttx to the juveniles of hatchery-reared non-toxic tiger puffer takifugu rubripes and measured the effects of ttx on brain corticotropin-releasing hormone (crh) mrna expression and plasma cortisol levels in comparison with effects in non-toxic juveniles. firstly, the reciprocal connections of crh and adrenocorticotropic hormone (acth) were confirmed by dual-label immunohistochemis ...201931580836
nephrometry score correlated with tumor proliferative activity int1 clear cell renal cell carcinoma-the tiger in the tall grasses versus the cat in plain sight. 201931576341
delineation of two new, highly similar species of taiwanese cylindera tiger beetles (coleoptera, carabidae, cicindelinae) based on morphological and molecular evidence.tiger beetles have been recognized primarily based on morphological characters. however, the variations of elytral maculation and coloration sometimes lead to misjudgment in species classification and the overlooking of the existence of cryptic species. recently, specimens of two endemic species of taiwanese cylindera, c. sauteri and c. pseudocylindriformis, exhibit morphologically recognizable forms, indicating that some undescribed species may exist. to clarify their taxonomic status, morpholo ...201931572044
twisted tale of the tiger: the case of inappropriate data and deficient science.publications in peer-reviewed journals are often looked upon as tenets on which future scientific thought is built. published information is not always flawless and errors in published research should be expediently reported, preferably by a peer-review process. we review a recent publication by gopalaswamy et al. (10.1111/2041-210x.12351) that challenges the use of "double sampling" in large-scale animal surveys. double sampling is often resorted to as an established economical and practical ap ...201931489264
the lhermitte-duclos disease: a rare bilateral cerebellar location of a rare pathology.dysplastic gangliocytoma or lhermitte-duclos disease is a rare disorder characterized by a slowly progressive unilateral tumour mass of the cerebellar cortex. it is probably hamartomatous, although the exact pathogenesis remains unknown. lhermitte-duclos disease was recently encountered to be part of a multiple hamartoma-neoplasia complex (cowden's syndrome). it typically presents in young adults, although it has been encountered at all ages. we present the case of bilateral cerebellar location ...201931489096
gut microbiota and their putative metabolic functions in fragmented bengal tiger population of nepal.bengal tigers (panthera tigris tigris) serve a pivotal role as an apex predator in forest ecosystems. to increase our knowledge on factors impacting the viability and health of this endangered species, we studied the gut microbiota in 32 individual bengal tigers from three geographically separated areas (chitwan national park (cnp), bardia national park (bnp) and suklaphanta wildlife reserve (swr)) in nepal, using noninvasive genetic sampling methods. gut microbiota influence the immune system, ...201931465520
india keeps a close eye on its tigers. 201931455912
tiger sharks eat songbirds: reply. 201931454415
changes in dna methylation during epigenetic-associated sex reversal under low temperature in takifugu rubripes.dna methylation has frequently been implicated in sex determination and differentiation in teleost species. in order to detect the dna methylation patterns established during sexual differentiation in tiger pufferfish t. rubripes, we performed comprehensive whole genome methylation sequencing and analyses of the gonads of male, female, and pseudo male. we obtained a total of 33.12, 32.44, and 31.60 gb clean data for male, female, and pseudo male, with a sequencing depth of 66.44×, 60.47× and 54. ...201931454376
tiger-like mycosis fungoides: an unusual clinical presentation of a rare variant of mycosis fungoides.mycosis fungoides (mf) is the most common type of cutaneous t-cell lymphoma. mycosis fungoides classically presents in the skin as patches, plaques, tumors, or erythroderma, progressing to involve the lymph nodes and peripheral blood. the many clinical variants, with different histologic patterns, and the subtle early clinical and histologic changes may delay early diagnosis and present a diagnostic challenge for clinicians. the greatest challenge in diagnosis is the pre-mycotic stage, which may ...201931450281
clinical implications of convergent procoagulant toxicity and differential antivenom efficacy in australian elapid snake venoms.australian elapid snakes are some of the most venomous snakes in the world and are unique among venomous snakes in having mutated forms of the blood clotting factor x in an activated form (fxa) as a key venom component. in human bite victims, an overdose of this activated clotting enzyme results in the systemic consumption of fibrinogen due to the large amounts of endogenous thrombin generated by the conversion of prothrombin to thrombin by venom fxa. within australian elapids, such procoagulant ...201931442586
microbial community structures and important associations between soil nutrients and the responses of specific taxa to rice-frog cultivation.rice-frog cultivation is a traditional farming system in china and has been reintroduced as an agricultural practice in china in recent years. the microbial community in paddy rhizospheric soils has attracted much attention because many microorganisms participate in functional processes in soils. in this study, illumina miseq sequencing-based techniques were used to investigate soil microbial communities and functional gene patterns across samples obtained by conventional rice cultivation (cr) a ...201931440215
a cytosolic sensor, pmddx41, binds double stranded-dna and triggers the activation of an innate antiviral response in the shrimp penaeus monodon via the sting-dependent signaling pathway.helicase ddx41 is a cytosolic sensor capable of detecting double-stranded dna in mammals. however, the function of ddx41 remains poorly understood in invertebrates. in a previous study, we identified the first ddx41 sensor in the black tiger shrimp penaeus monodon (pmddx41) and showed that it played a role in anti-viral response. in the present study, we demonstrated that pmddx41 was localized in the cytoplasm of shrimp hemocytes. however, pmddx41 was localized in both the cytoplasm and nucleus ...201931552028
generics designate kinds but not always essences.people believe that some categories are kinds with reliable causal structure and high inductive potential (e.g., tigers). widely endorsed theories propose that people are biased to assume kinds are essential, and so naturally determined by internal causal properties. generic language (e.g., "men like sports") is 1 mechanism thought to evoke this bias. we propose instead that generics principally designate that categories are kinds. participants can entertain diverse causal structures in the pres ...201931548415
genome organization and definition of the penaeus monodon viral responsive protein 15 (pmvrp15) promoter.the viral responsive protein 15 from the black tiger shrimp penaeus monodon (pmvrp15) is a highly responsive gene upon white spot syndrome virus (wssv) challenge. it is identified from hemocyte and important for wssv trafficking and assembly. however, the knowledge of pmvrp15 gene regulation is limited. in the present study, the genome organization and 5'upstream promoter sequences of pmvrp15 gene were investigated. the pmvrp15 gene was found to contain 4 exons interrupted by 3 introns and the s ...201931412281
[discussion on opposing needling combined with dragon-tiger fighting needling for chronic pelvic inflammation].opposing needling is an acupoint selection method of acupuncture recorded in huangdi neijing (yellow emperor 's internal classic). and the first record of dragon-tiger fighting needling is found in the zhenjiu daquan (great compendium on acupuncture and moxibustion), it is a compound reinforcing and reducing manipulation of tonification-purgation method. both of them are widely used in the treatment of pain syndrome, which are mainly for nerve system and musculoskeletal diseases and seldom for g ...201931397135
comparison of physicochemical characteristics and fibril formation ability of collagens extracted from the skin of farmed river puffer (takifugu obscurus) and tiger puffer (takifugu rubripes).acid-soluble collagen (asc) and pepsin-soluble collagen (psc) from the skin of river puffer (asc-rp and psc-rp) and tiger puffer (asc-tp and psc-tp) were extracted and physicochemically examined. denaturation temperature (td) for all the collagens was found to be 25.5-29.5 °c, which was lower than that of calf skin collagen (35.9 °c). electrophoretic patterns indicated all four samples were type i collagen with molecular form of (α1)2α2. ftir spectra confirmed the extracted collagens had a tripl ...201931394862
behavioral repertoire assessment of bengal tigers (panthera tigris) with focus on thermoregulatory behavior.the behavioral repertoire and environmental feature needs for thermoregulatory comfort have not been reported in the literature for large captive exotics. an observational study was done to investigate the behavioral repertoire of tigers via continuous observation, while focusing on thermoregulatory behavior, in order to examine behavioral and thermoregulatory needs of these animals, and inform microclimatic landscape design for thermal comfort. nine bengal tigers (n = 6 females, n = 3 males) we ...201931309283
bi-allelic tars mutations are associated with brittle hair phenotype.brittle and "tiger-tail" hair is the diagnostic hallmark of trichothiodystrophy (ttd), a rare recessive disease associated with a wide spectrum of clinical features including ichthyosis, intellectual disability, decreased fertility, and short stature. as a result of premature abrogation of terminal differentiation, the hair is brittle and fragile and contains reduced cysteine content. hypersensitivity to uv light is found in about half of individuals with ttd; all of these individuals harbor bi- ...201931374204
induction of gonadal development in protogynous grouper with orally delivered fsh dna.the availability of sexually mature fish often dictates the success of its captive breeding. in this study, we induced reproductive development in juvenile protogynous tiger grouper through oral administration of a plasmid (p) containing an engineered follicle-stimulating hormone (fsh). an expression construct (pcdna3.1) was designed to express a single-chain fsh consisting of giant grouper fsh β-subunit and glycoprotein subunit-α (cgα), linked by the carboxy-terminal peptide (ctp) sequence from ...201931372794
differential transcriptome analysis of the disease tolerant madagascar-malaysia crossbred black tiger shrimp, penaeus monodon hepatopancreas in response to acute hepatopancreatic necrosis disease (ahpnd) infection: inference on immune gene response and interaction.penaeus monodon is the second most widely cultured marine shrimp species in the global shrimp aquaculture industry. however, the growth of p. monodon production has been constantly impaired by disease outbreaks. recently, there is a lethal bacterial infection, known as acute hepatopancreatic necrosis disease (ahpnd) caused by vibrio parahaemolyticus ahpnd strain (vpahpnd), which led to mass mortalities in p. monodon. unfortunately, there is still insufficient knowledge about the underlying immun ...201931372182
pmeea1, the early endosomal protein is employed by yhv for successful infection in penaeus monodon.yellow head disease (yhd) is an infectious disease of penaeus monodon which is caused by the yellow head virus (yhv). yhv infection invariably leads to 100% shrimp mortality within 3-5 days. currently, an effective method to prevent or cure shrimp from yhv infection has not been elucidated. therefore, the molecular mechanism underlying yhv infection should be examined. in this study, early endosome antigen 1 (eea1) protein that was involved in the tethering step of the vesicle and early endosome ...201931678535
acute toxicity of an organophosphate insecticide sumithion to striped catfish pangasianodon hypophthalmus.sumithion is widely used for crop safety and eradication of tiger bugs (cicindela spp.) from larval rearing aquaculture ponds. to satisfy the high demand of fries and fingerlings of widely cultured species striped catfish, spawns are produced in large scale in the hatcheries through hormone induced breeding, and subsequently these spawns are reared in nursery ponds and marketed to fingerlings vendors for stocking in grow-out ponds. considering the importance of healthy fries and fingerlings the ...201931673497
india's tigers seem to be a massive success story - many scientists aren't sure. 201931664220
open data could save more tigers. 201931664217
direct evidence of an extra-intestinal cycle of toxoplasma gondii in tigers (panthera tigris) by isolation of viable strains.toxoplasmosis is one of the most common zoonotic diseases in the world. felines excrete environmentally resistant toxoplasma gondii oocysts. however, there is no direct evidence to prove tigers are the intermediate host of t. gondii. here, we show that, igg antibodies to t. gondii in 80% (8/10) of captive tigers. two viable t. gondii strains (toxodb genotype #9) were isolated by bioassay in mice using striated muscles of two tigers (tiger#3 and tiger#8). additionally, mice were confirmed as t. g ...201931661400
genomic characterization of a novel astrovirus identified in amur tigers from a zoo in china.astroviruses (astvs) have a very wide range of hosts and are associated with enteric and extra-enteric disease in mammals and birds. cross-species transmission of astvs has been observed frequently. in the present study, the genome of a novel astrovirus from amur tigers (panthera tigris) from a zoo in china was characterized and was found to have the typical genomic features of other mammal astvs. it showed the highest nucleotide sequence similarity (46.1-87.3% identity) to astvs from cats, indi ...201931616994
[pedigree analysis of c19orf12 p.asp18tyr mutation in a family with mitochondrial membrane protein associated neurodegeneration].objective: to explore the clinical features and analyze the chromosome 19 open reading frame 12(c19orf12) gene mutation of a family with mitochondrial membrane protein-associated neurodegeneration (mpan). methods: the pedigree diagnosed as neurodegeneration with brain iron accumulation (nbia) in henan provincial people's hospital in may 2018 was collected, and clinical data of the patients in this family was further analyzed. furthermore, whole exome sequencing (wes) was employed to identify the ...201931607023
tiger mosquitoes tackled in a trial. 201931358926
six decades of preventing and treating childhood anxiety disorders: a systematic review and meta-analysis to inform policy and practice.anxiety disorders are the most prevalent childhood mental disorders. they also start early and persist, causing high individual and collective costs. to inform policy and practice, we therefore asked: what is the best available research evidence on preventing and treating these disorders?201931315926
do we know the correct dose of tiger snake antivenom? 201931313488
the road to deforestation: edge effects in an endemic ecosystem in sumatra, indonesia.worldwide, roads are a main driver of deforestation and degradation as they increase forest access along the forest edge. in many tropical areas, unofficial roads go unreported and unrecorded, resulting in inaccurate estimates of intact forested areas. this is the case in central sumatra, which boasts populations of critically endangered sumatran elephants (elephas maximus sumatrensis), tigers (panthera tigris sumatrae) and other endemic flora and fauna that make the area globally unique. howeve ...201931260478
chemical composition of industrially and laboratory processed cyperus esculentus rhizomes.we report here the results of the study of the chemical composition of cyperus esculentus rhizomes. ethanolic extracts have been separated by column chromatography and analyzed by magnetic resonance spectroscopy and mass spectrometry. quercetin, stigmasterol, and linoleic and oleic acid glycerol esters, together with 4-chlorobutyl oleate, oleamide, myricetin, tyramine and n-feruloyltyramine, found for the first time in cyperus esculentus rhizomes, have been isolated and quantified in the extract ...201931253254
molecular characterization and functional analysis of akirin from black tiger shrimp (penaeus monodon).akirin, which are members of the nf-κb signaling pathway, play critical roles in regulating the expression of antimicrobial peptides. in the present study, the akirin gene from penaeus monodon was identified from a transcriptome database and designated as pmakirin. the complete sequence of the pmakirin cdna was 1508 bp, encoding a protein of 213 amino acids, and it showed 99% amino acid identity to the litopenaeus vannamei akirin. two predicted nuclear localization signals (nlss) were found, and ...201931541777
role of clathrin assembly protein-2 beta subunit during white spot syndrome virus infection in black tiger shrimp penaeus monodon.white spot syndrome virus (wssv) is one of the most lethal viruses severely affecting shrimp industry. this disease can cause 100% mortality of farmed shrimp within a week. this work aims to characterize clathrin assembly proteins in penaeus monodon and investigate their roles in wssv entry. in general, clathrin assembly proteins form complexes with specific receptors and clathrins, leading to clathrin-mediated endocytosis. adaptor protein 2 (ap-2), which is responsible for endocytosis at plasma ...201931530841
evidence for functional gabaa but not gabac receptors in mouse cone photoreceptors.at the first retinal synapse, horizontal cells (hcs) contact both photoreceptor terminals and bipolar cell dendrites, modulating information transfer between these two cell types to enhance spatial contrast and mediate color opponency. the synaptic mechanisms through which these modulations occur are still debated. the initial hypothesis of a gabaergic feedback from hcs to cones has been challenged by pharmacological inconsistencies. surround antagonism has been demonstrated to occur via a modul ...201931199212
cloning, overexpression, purification, and modeling of a lectin (rhinocelectin) with antiproliferative activity from tiger milk mushroom, lignosus rhinocerus.a lectin gene from the tiger milk mushroom lignosus rhinocerus tm02® was successfully cloned and expressed via vector pet28a in escherichia coli bl21(de3). the recombinant lectin, rhinocelectin, with a predicted molecular mass of 22.8 kda, was overexpressed in water-soluble form without signal peptide and purified via native affinity chromatography ni-nta agarose. blast protein analysis indicated the lectin to be homologous to jacalin-related plant lectin. in its native form, rhinocelectin exist ...201931190445
pre-hispanic fishing practices in interfluvial amazonia: zooarchaeological evidence from managed landscapes on the llanos de mojos savanna.recent evidence suggests the existence of pre-hispanic fisheries in savanna areas of the amazon basin. how these fisheries may have functioned is still poorly known. although many studies have drawn attention to how pre-hispanic inhabitants of these savannas managed to deal with excess water, little attention has been paid to understanding how large and permanent populations were sustained during long periods of drought. in the llanos de mojos, one of the largest savannas in south america, the l ...201931091235
taqman real-time and conventional nested pcr tests specific to yellow head virus genotype 7 (yhv7) identified in giant tiger shrimp in australia.in 2013, a unique seventh yellow head virus genotype (yhv7) was detected in black tiger shrimp (penaeus monodon) broodstock that suffered high mortality following their capture from joseph bonaparte gulf (jbg) in northern australia. to assist with its diagnosis and assessment of its distribution, prevalence and pathogenicity, yhv7-specific taqman real-time qpcr and conventional nested pcr primer sets were designed to orf1b gene sequences divergent from the other yhv genotypes. using high (≥108) ...201931276700
distribution of lymph node metastases in esophageal carcinoma [tiger study]: study protocol of a multinational observational study.an important parameter for survival in patients with esophageal carcinoma is lymph node status. the distribution of lymph node metastases depends on tumor characteristics such as tumor location, histology, invasion depth, and on neoadjuvant treatment. the exact distribution is unknown. neoadjuvant treatment and surgical strategy depends on the distribution pattern of nodal metastases but consensus on the extent of lymphadenectomy has not been reached. the aim of this study is to determine the di ...201931272485
bite performance and feeding behaviour of the sand tiger shark carcharias taurus.this study examines the feeding behaviour and kinematics of three sub-adult sand tiger sharks carcharias taurus on display at mystic aquarium (mystic, connecticut, usa). using high-speed video data from 52 bites, we identify kinematic variables associated with the expansive and compressive phases of the bite. the mean bite duration from the onset of the expansive phase to the conclusion of the compressive phase is mean (± se) 0.14 ± 0.01 s and across the 10 fastest bites of each individual, the ...201931265127
large felid leucoencephalomyelopathy in a sumatran tiger (panthera tigris sumatrae) from an australian zoo.the clinicopathological features of a case consistent with large felid leucoencephalomyelopathy are described in a 19-year-old, zoo-based sumatran tiger in which degenerative vertebral disease, renal insufficiency, diaphragmatic hernia and cataracts were comorbid. the principal presenting sign was ataxia, with concurrent deterioration of vertebral stiffness and vision loss. histological features included marked destruction of the white matter, the formation of large, bizarre astrocytes and accum ...201931209862
molecular characterization of the nk-lysin in a teleost fish, boleophthalmus pectinirostris: antimicrobial activity and immunomodulatory activity on monocytes/macrophages.nk-lysin (nkl) is a cationic host defense peptide that plays an important role in host immune responses against various pathogens. however, the immunomodulatory activity of nkl in fishes is rarely investigated. in this study, we characterized a cdna sequence encoding an nk-lysin homolog (bpnkl) from the fish, mudskipper (boleophthalmus pectinirostris). sequence analysis revealed that bpnkl is most closely related to tiger puffer (takifugu rubripes) nkl. bpnkl transcript was detected in all the t ...201931200076
novel treatment of severe radial artery spasm using "homemade sheathless technique": a case report.transradial access (tra) is common for cardiac catheterization, but radial artery spasm (ras) is suggested to be highlighted. severe radical artery spasm could be solved by a relative novel approach called "sheathless technique," using a tiger diagnostic catheter.201930921221
novel pank2 mutation discovered among south east asian children living in thailand affected with pantothenate kinase associated neurodegeneration.pantothenate kinase-associated neurodegeneration (pkan) is linked to brain iron accumulation caused by pank2 gene mutation. despite the importance of genetic testing to confirm pkan and identify at risk parents, genetic screening is financially burdensome for developing countries like thailand. because genetic screeners are expensive and not reimbursed by the universal health care coverage system, they are not typically performed. to investigate clinical symptoms, radiological findings and mutat ...201931088771
glottal function index questionnaire for screening of pediatric dysphonia.to assess the diagnostic value of lithuanian version of glottal function index (gfi-lt) questionnaire in pediatric dysphonia screening.201931085464
amelioration of dystonic opisthotonus in pantothenate kinase-associated neurodegeneration syndrome with absent "eye-of-the-tiger" sign following bilateral pallidal deep brain stimulation. 201931061845
metabolite profiling of epinephelus fuscoguttatus infected with vibriosis reveals omega 9 as potential metabolite biomarker.in this study, we report the starvation effect and vibriosis infection on a tropical fish, the tiger grouper (epinephelus fuscoguttatus). the tiger groupers were infected with vibrio vulnificus for 21 days. gas chromatography-mass spectrometry combined with multivariate analysis was used to assess the variation in metabolite profiles of e. fuscoguttatus. metabolite productions in infected fishes were significantly influenced by fatty acid production. the omega 9 (ω-9) was abundant under the chal ...201930915615
chitin extraction from shrimp waste by liquid fermentation using an alkaline protease-producing strain, brevibacillus parabrevis.in this study, an extracellular protease, but no chitinolytic enzyme-producing strain, brevibacillus parabrevis tku046, has been isolated and analyzed for the deproteinization testing of shrimp waste by liquid fermentation. deproteinization assays of shrimp waste with this microbe showed 95% protein removal after 4 days fermentation. the efficiency of chitin extraction by b. parabrevis tku046 on wastes of three shrimp species were also investigated in which the highest deproteinization was found ...201930904526
mitral valve bypass: another extra-anatomic solution for another tiger territory? 201930901802
anaesthetic efficacy and physiological response of clove oil and lidocaine-hcl on river puffer, takifugu obscurus and tiger puffer, t. rubripes.the effects of the anaesthetic agents, clove oil and mixture of clove oil with lidocaine-hcl were evaluated on river puffer, takifugu obscurus and tiger puffer, t. rubripes. anaesthesia times of clove oil were affected by water temperature (20℃, 24℃, and 28℃) and salinity (10, 20, and 30 ppt). anaesthesia times of mixed samples were significantly similar with regard to exposure and recovery times, and all samples satisfied anaesthesia criteria (exposure time within 3 min and recovery time within ...201931049469
comparative analysis of mitochondrial dna datasets indicates that toxascaris leonina represents a species complex.toxascaris leonina is one of the most common intestinal parasites of canids and felids. in this study, we characterised the entire mitochondrial (mt) genome sequence of t. leonina from the cheetah and compared it with that of t. leonina from the dog.201931046831
rediscovering the axolotl as a model for thyroid hormone dependent development.the mexican axolotl (ambystoma mexicanum) is an important model organism in biomedical research. much current attention is focused on the axolotl's amazing ability to regenerate tissues and whole organs after injury. however, not forgotten is the axolotl's equally amazing ability to thwart aspects of tissue maturation and retain juvenile morphology into the adult phase of life. unlike close tiger salamander relatives that undergo a thyroid hormone regulated metamorphosis, the axolotl does not ty ...201931031711
c-type lectin response to bacterial infection and ammonia nitrogen stress in tiger shrimp (penaeus monodon).c-type lectins (ctls) are pattern recognition receptors (prrs) that are important in invertebrate innate immunity for the recognition and elimination of pathogens. although they were reported in many shrimp, c-type lectins subfamily contain a large number of members with different functions that need to research in deep. in this present study, a new type of ctl, pmcl1 with 861 bp long full-length cdna, that encodes a protein with 164-amino acid from a 495-bp open reading frame, was isolated and ...201931028898
impact of prey occupancy and other ecological and anthropogenic factors on tiger distribution in thailand's western forest complex.despite conservation efforts, large mammals such as tigers (panthera tigris) and their main prey, gaur (bos gaurus), banteng (bos javanicus), and sambar (rusa unicolor), are highly threatened and declining across their entire range. the only large viable source population of tigers in mainland southeast asia occurs in thailand's western forest complex (wefcom), an approximately 19,000 km2 landscape of 17 contiguous protected areas.we used an occupancy modeling framework, which accounts for imper ...201930891192
dispersal of amur tiger from spatial distribution and genetics within the eastern changbai mountain of china.population dispersal and migration often indicate an expanded habitat and reduced inbreeding probability, and to some extend reflects improvement in the condition of the population. the amur tiger population in the northern region of the changbai mountain in china mostly distributes along the sino-russian border, next to the population in southwest primorye in russia. the successful dispersal westward and transboundary movement are crucial for the persistence of the amur tiger in this area. this ...201930891189
sampling related individuals within ponds biases estimates of population structure in a pond-breeding amphibian.effective conservation and management of pond-breeding amphibians depends on the accurate estimation of population structure, demographic parameters, and the influence of landscape features on breeding-site connectivity. population-level studies of pond-breeding amphibians typically sample larval life stages because they are easily captured and can be sampled nondestructively. these studies often identify high levels of relatedness between individuals from the same pond, which can be exacerbated ...201930962914
response of hemocytes profile in the black tiger shrimp (penaeus monodon) against vibrio harveyi induced by xylocarpus granatum leaves extract.the present study investigated hemocytes profile of black tiger shrimp (penaeus monodon) induced with xylocarpus granatum leaves extract to protect against vibrio harveyi infection.201932546921
a high-density genetic linkage map and qtl mapping for sex in black tiger shrimp (penaeus monodon).the black tiger shrimp, penaeus monodon, is important in both fishery and aquaculture and is the second-most widely cultured shrimp species in the world. however, the current strains cannot meet the market needs in various cultural environments, and the genome resources for p. monodon are still lacking. restriction-site associated dna sequencing (radseq) has been widely used in genetic linkage map construction and in quantitative trait loci (qtl) mapping. we constructed a high-density genetic li ...201931024632
the bias of combining variables on fish's aggressive behavior studies.quantifying animal aggressive behavior by behavioral units, either displays or attacks, is a common practice in animal behavior studies. however, this practice can generate a bias in data analysis, especially when the variables have different temporal patterns. this study aims to use bayesian hierarchical linear models (b-hlms) to analyze the feasibility of pooling the aggressive behavior variables of four cichlids species. additionally, this paper discusses the feasibility of combining variable ...201931022507
dna barcoding of shrimps from a mangrove biodiversity hotspot.a total of 74 shrimp specimens were sequenced at a 584 bp segment of the cytochrome oxidase subunit i (coi) gene to examine patterns of dna barcode variation in a mangrove biodiversity hotspot. the maximum likelihood tree, barcode gap analysis, automatic barcode gap discovery analysis and sequence comparisons with data available from barcode of life data system and genbank recovered 18 taxa of which 15 were identified to species level, 2 at genus level and a single taxon at order level. two deep ...201931012766
establishment of a monoclonal antibody pmab-231 for tiger podoplanin.podoplanin (pdpn), also known as t1alpha, has been used as a lung type i alveolar cell marker in the pathophysiological condition. although we have established several monoclonal antibodies (mabs) against mammalian pdpns, mabs against tiger pdpn (tigpdpn), which are useful for immunohistochemical analysis, remain to be developed. in this study, we immunized mice with tigpdpn-overexpressing chinese hamster ovary (cho)-k1 cells (cho/tigpdpn) and screened hybridomas producing mabs against tigpdpn u ...201931009336
citizen science reveals female sand tiger sharks (carcharias taurus) exhibit signs of site fidelity on shipwrecks. 201931009086
a novel homozygous variation in the pank2 gene in two persian siblings with atypical pantothenate kinase associated neurodegeneration.pantothenate kinase-associated neurodegeneration (pkan) is an autosomal recessive disorder that is caused by variation in pantothenate kinase-2 gene (pank2) gene on chromosome 20. the common presentation of this disease includes progressive dystonia, parkinsonism, retinopathy, cognitive impairment, and spasticity. the typical magnetic resonance imaging finding is eye of the tiger sign in globus pallidus and not pathogenic and not found in all patients. in the present study, we describe two sibli ...201930996846
from climate zone to microhabitat-environmental factors affecting the coastal distribution of tiger beetles (coleoptera: cicindelidae) in the south-eastern european biodiversity hotspot.tiger beetles (coleoptera: cicindelidae) are predatory insects usually occurring in various sandy habitats. in south-eastern europe, especially in lowland areas located close to the sea coast, the diversity of cicindelidae is one of the highest in the palaearctic realm. although previous studies conducted in different areas of the world show that many species are habitat specialists, unfortunately little is known about environmental factors affecting the diversity and distribution of tiger beetl ...201930993037
intranasal administration of lignosus rhinocerotis (cooke) ryvarden (tiger milk mushroom) extract attenuates airway inflammation in murine model of allergic asthma.asthma is a chronic inflammatory disorder in the airways that involves the activation of cells and mediators. lignosus rhinocerotis (cooke) ryvardan or tiger milk mushroom is a medicinal mushroom that is traditionally used to treat inflammatory diseases including asthma. in this study, the protective effects of intranasal administration of l. rhinocerotis extract (lre) in ovalbumin (ova)-induced airway inflammation mouse model were investigated. mice were sensitized by intraperitoneal (i.p) inje ...201930988772
comparison of tigers' fecal glucocorticoids level in two extreme habitats.application of different antibodies and extraction methods results in a wide range of steroid metabolite concentrations obtained during noninvasive hormones monitoring. it makes regional comparisons of steroid concentration very difficult. we compared three methods for extraction of glucocorticoids metabolites in tiger feces to examine correct stress level in bengal and amur tigers in india and russia respectively. the results obtained with three different extraction methods correlate with each ...201930969977
simultaneous authentication of species identity and geographical origin of shrimps: untargeted metabolomics to recurrent biomarker ions.mandatory disclosure of the species identity, production method, and geographical origin are embedded in the regulations and traceability systems, governing international seafood trade. a high-resolution mass spectrometry-based metabolomics approach could simultaneously authenticate the species identity and geographical origin of commercially important shrimps. the highly innovative approach spared the need for multiple testing methods which are in routine use currently. a robust chemometric mod ...201930967241
production of a monoclonal antibody against of muskellunge (esox masquinongy) igm heavy chain and its use in development of an indirect elisa for titrating circulating antibodies against vhsv-ivb.this study reports the development of a monoclonal antibody (designated 3b10) against the muskellunge (esox masquinongy) igm. the 3b10 monoclonal antibody (mab) belongs to the igg3 kappa isotype. western blotting demonstrated that 3b10 mab reacted primarily to muskellunge igm heavy chain. 3b10 also reacted strongly with the igm heavy chain of other esocids, including the northern pike (esox lucius), tiger muskellunge (e. masquinongy x e. lucius), and, to a much lesser extent, the chain pickerel ...201930858097
a transgenic inducible gfp extracellular-vesicle reporter (tiger) mouse illuminates neonatal cortical astrocytes as a source of immunomodulatory extracellular vesicles.extracellular vesicles (evs) are cellular derived particles found throughout the body in nearly all tissues and bodily fluids. evs contain biological molecules including small rnas and protein. evs are proposed to be transferred between cells, notably, cells of the immune system. tools that allow for in vivo ev labeling while retaining the ability to resolve cellular sources and timing of release are required for a full understanding of ev functions. fluorescent ev fusion proteins are useful for ...201930816224
this shoe, that tiger: semantic properties reflecting manual affordances of the referent modulate demonstrative use.demonstrative reference is central to human communication. but what influences our choice of demonstrative forms such as "this" and "that" in discourse? previous literature has mapped the use of such "proximal" and "distal" demonstratives onto spatial properties of referents, such as their distance from the speaker. we investigated whether object semantics, and specifically functional properties of referents, also influence speakers' choices of either demonstrative form. over two experiments, we ...201930615694
magnetic resonance imaging, susceptibility weighted imaging and quantitative susceptibility mapping findings of pantothenate kinase-associated neurodegeneration.pantothenate kinase-associated neurodegeneration (pkan) is extremely rare. in this study, we aimed to evaluate the magnetic resonance imaging (mri) findings of pkan patients. conventional mri and susceptibility weighted imaging (swi) sequences and quantitative susceptibility mapping (qsm) maps of six patients from three pkan families and eight healthy male volunteers were retrospectively analyzed. iron content was represented by qsm values. the typical eye-of-the-tiger sign (n = 4) and hyperinte ...201930392836
audit of toxic effects of body paint in the tiger dancers (hulivesha) of mangalore, india: an investigational study.tiger dancing or hulivesha, where the volunteers paint their bare body like a tiger and dance in a ritual during the navarathri festival in mangalore, india. there are no scientific studies done with the hulivesha dancers at all, and therefore, we investigated the adverse effects of painting body like a tiger and dancing in the volunteers.201930501442
high-dose chemotherapy in relapsed or refractory metastatic germ-cell cancer: the scotland experience.to report outcomes from high-dose chemotherapy (hdct) and autologous stem-cell transplantation (asct) for metastatic germ-cell cancer in scotland.201930563754
caudal musculature in ground sharks, carcharhiniformes, with remarks on their phylogenetic interrelationships.relationships among families of the shark order carcharhiniformes are still being debated, for example, in relation to the inclusion of hammerhead sharks (sphyrnidae) and the tiger shark (galeocerdo cuvier) in the carcharhinidae. previous morphological studies have not analyzed the phylogenetic significance of axial caudal muscles among carcharhiniforms. the authors therefore describe here the axial muscles of the tail region (m. epaxialis, m. obliquus superioris, m. obliquus inferioris, m. flex ...201930556950
natural concurrent infection of vibrio harveyi and v. alginolyticus in cultured hybrid groupers in malaysia.in september 2016, a marine fish farm operator in selangor, malaysia, reported a disease outbreak affecting juvenile hybrid groupers (camouflage grouper epinephelus polyphekadion × tiger grouper e. fuscoguttatus). the average daily mortality was 120 fish, resulting in a cumulative mortality rate of 29% within 10 d. the affected hybrid groupers displayed lethargy, excessive mucus production, rotten fins, congestion of livers and kidneys, and enlargement of spleens. microscopically, general conges ...201930536485
functional characterization of a cdna encoding piwi protein in penaeus monodon and its potential roles in controlling transposon expression and spermatogenesis.piwi proteins comprise a subfamily of argonaute that plays a major role in germline development by association with a distinct class of small rnas called piwi interacting rna (pirna). although the functions of piwi in the development of germline cells as well as transposon regulation were reported in a number of mammalians and insects, developmental expression and function of piwi subfamily in crustaceans is poorly known. this study is aimed at cloning and characterization of a piwi cdna in the ...201930529395
tn-core: a toolbox for integrating tn-seq gene essentiality data and constraint-based metabolic modeling.the design of synthetic cells requires a detailed understanding of the relevance of genes and gene networks underlying complex cellular phenotypes. transposon-sequencing (tn-seq) and constraint-based metabolic modeling can be used to probe the core genetic and metabolic networks underlying a biological process. integrating these highly complementary experimental and in silico approaches has the potential to yield a highly comprehensive understanding of the core networks of a cell. specifically, ...201930525460
comparative effect of slow and rapid freezing on sperm functional attributes and oxidative stress parameters of goat spermatozoa cryopreserved with tiger nut milk extender.comparative effect of slow and rapid freezing on sperm functional attributes and oxidative stress parameters of goat spermatozoa cryopreserved with tiger nut milk (tnm) extender was examined in this study. pooled semen samples obtained from west african dwarf (wad) goat bucks were diluted with tris-based extenders containing different levels of tnm (0, 5, 10, 15 and 20 ml/100 ml extender). the diluted semen samples were subjected to slow and rapid freezing for a period of 7 days and thereafter e ...201930570173
guidelines for patients with acute uncomplicated cystitis may not be a paper tiger: a call for its implementation in clinical routine. 201930564871
the complete mitochondrial genome of parantica sita sita (lepidoptera: nymphalidae: danainae) revealing substantial genetic divergence from its sibling subspecies p. s. niphonica.currently, there are two subspecies of the chestnut tiger butterfly (parantica sita) recognized in china. p. s. sita is widely distributed in southwest china and p. s. niphonica in taiwan. periodically, taiwan island and the chinese mainland have been connected and separated because of sea level changes caused by pleistocene glaciations, most likely influencing the genetic structure among p. sita populations on both sides of the taiwan strait. also, p. s. niphonica's well-documented long-distanc ...201930391439
mapping and predicting forest loss in a sumatran tiger landscape from 2002 to 2050.riau province in central sumatra, with its peatland, lowland, and montane forest habitats, was once a stronghold for sumatran tiger (panthera tigris sumatrae) populations. today, riau may have one of the highest deforestation rates in the world and wildlife populations are dwindling, with natural forest now comprising approximately only 18% of the province, mostly contained within protected areas. agriculture (acacia, rubber, and oil palm) makes up the majority of riau's land cover and deforesta ...201930368149
clinical perspective on dysplastic gangliocytoma of the cerebellum (lhermitte-duclos disease).dysplastic gangliocytoma of the cerebellum, also called lhermitte-duclos disease (ldd), is known as a rare, benign brain tumor. around 200 cases have been reported.201930366139
acoustic analysis of voice in school teachers.the term 'voice' is the acoustic energy generated from the vocal tract that are characterized by their dependence on vocal fold vibratory pattern. teachers as professional voice users are afflicted with dysphonia and are discouraged with their jobs and seek alternative employment. loud speaking and voice straining may lead to vocal fatigue and vocal fold tissue damage. the objective of the study is to assess the quality of voice of school teachers before and after teaching practice.201930381759
gonadal response of juvenile protogynous grouper (epinephelus fuscoguttatus) to long-term recombinant follicle-stimulating hormone administration†.the role of follicle-stimulating hormone (fsh) in the gonadal development of protogynous hermaphroditic grouper (epinephelus fuscoguttatus) was investigated. recombinant giant grouper (e. lanceolatus) fsh (rggfsh) was produced in yeast. its receptor-binding capacity and steroidogenic potency were confirmed in vitro. weekly injections of rggfsh to juvenile tiger grouper for 8 weeks (100 μg/kg body weight, bw) resulted in significantly larger and more advanced oocytes (cortical alveolar stage vs p ...201930371741
the influence of 15-week exercise training on dietary patterns among young adults.little is currently known about how exercise may influence dietary patterns and/or food preferences. the present study aimed to examine the effect of a 15-week exercise training program on overall dietary patterns among young adults.201930659257
circulating microvesicles in snakebite patients with microangiopathy.venom-induced consumption coagulopathy is a common consequence of snake envenoming that can lead to life-threatening hemorrhage, and is associated with microangiopathic hemolytic anemia (maha), acute kidney injury and thrombocytopenia. the role of microvesicles (mv) in snakebite patients has not been previously investigated.201930656285
tiger man sign in sarcoid myopathy. 201930637499
nutritional evaluation on lignosus cameronensis c. s. tan, a medicinal polyporaceae.sclerotial powder of a cultivated species of the tiger milk mushroom, lignosus cameronensis was analysed for its nutritional components and compared against species of the same genus, lignosus rhinocerus and lignosus tigris. all three species have been used by indigenous tribes in peninsular malaysia as medicinal mushrooms. content of carbohydrate, fibre, mineral, amino acid, palatable index, fat, ash and moisture were determined. l. cameronensis sclerotial material consists of carbohydrate (79. ...201930629799
ecdysone inducible gene e75 from black tiger shrimp penaeus monodon: characterization and elucidation of its role in molting.molting is controlled by ecdysteroids, which are synthesized and secreted by the y-organ in crustaceans. ecdysone inducible gene, e75, is an early-response gene in the 20-hydroxyecdysone (20e) signaling pathway, with crucial roles in arthropod development. complementary dna (cdna) encoding penaeus monodon e75 (pme75) was cloned using rt-pcr and race. pme75 cdna was 3526 bp long and encoded a 799-amino acid protein. tissue distribution analysis showed that pme75 was expressed ubiquitously in sele ...201930618055
functional characterization of the epoxidase gene, li_epo1 (cyp341b14), involved in generation of epoxyalkene pheromones in the mulberry tiger moth lemyra imparilis.epoxidation of alkenes derived from essential fatty acids is a key step in the biosynthesis of sex pheromones in moth species that utilize alkenyl sex pheromones. the position of the epoxy ring in the pheromone molecule differs depending on the species, thereby conferring diversities on sex pheromones. to date, only one pheromone gland (pg)-specific epoxidase, hc_epo1 (cyp341b14), has been reported. hc_epo1, which was identified from an arctiid moth hyphantria cunea, catalyzes the epoxidation of ...201930742902
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