Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
expression profiling of endocrine-disrupting compounds using a customized cyprinus carpio cdna microarray.exposure to a variety of anthropogenic compounds has been shown to interfere with normal development, physiology, and reproduction in a wide range of organisms, both in laboratory studies and wildlife. we have developed a cyprinus carpio cdna microarray consisting of endocrine-related genes. in the current study, we investigated the applicability of this microarray (1) to study the molecular effects induced by exposure to a variety of endocrine-disrupting compounds (edcs) in fish and (2) to disc ...200616835292
haematological responses of acute nitrite exposure in walleye (sander vitreus).nitrite (no2-) is a toxic intermediary of the bacterial oxidation of nitrogenous wastes (e.g. ammonia) in an aquatic environment. it becomes most lethal when oxygen becomes limited due to high fish densities or in the presence of high bacterial activity due to waste build-up-both situations commonly found in intensive aquaculture. to date however, little is known about how this toxin affects the physiology of walleye, an intended culture species, particularly in intensive re-circulating systems. ...200616806526
phosphorylation of cl-, hco3-activated mg2+-atpase from carp (cyprinus carpio l.) brain plasma membranes. 200616776067
characterization of transthyretin in the pacific bluefin tuna, thunnus orientalis.a cdna encoding transthyretin was cloned from the pacific bluefin tuna (thunnus orientalis). this cdna contains a complete open reading frame encoding 151 amino acid residues. the deduced amino acid sequence is 81% and 55% identical to the gilthead seabream and common carp forms, respectively, and 33-39% to mammalian, reptilian, and amphibian forms. a 1.0-kb transcript was found in the the liver and ovary; the liver is the main source of this protein. analysis of triiodo-l-thyronine (t(3)) and l ...200616766863
postoperative outcomes for patients undergoing elective revascularization for critical limb ischemia and intermittent claudication: a subanalysis of the coronary artery revascularization prophylaxis (carp) trial.to determine the perioperative mortality, myocardial infarction rate, and long-term survival of patients with critical limb ischemia (cli) compared with those with intermittent claudication (ic) within a cohort selected for significant coronary artery disease, a secondary analysis was conducted of a prospective, randomized, multicenter trial of coronary artery revascularization prophylaxis (carp) before peripheral vascular surgery. this multicenter trial was sponsored by the cooperative studies ...200616765234
dynamics of cadmium accumulation and effects in common carp (cyprinus carpio) during simultaneous exposure to water and food (tubifex tubifex).in this study, the influence of exposure time and concentration on the accumulation of cadmium and the occurrence of adverse effects was investigated when carp were simultaneously exposed through contaminated water and food. as exposure concentrations increased (9, 105, and 480 microg/l through water and 9.5, 122, and 141 microg/g dry weight through food) the accumulation pattern of cadmium changed, with progressively more cadmium being accumulated in the liver and especially the kidney and less ...200616764474
heavy metals content and microbiological quality of carp (cyprinus carpio, l.) muscle from two southwestern slovak fish farms.the purpose of this study was to assess concentration and correlation of selected heavy metals and level of some microbiological indicators (total bacteria count-tbc, mesophilic anaerobic sporulating bacteria-masb) in the muscle of common carp (cyprinus carpio). fish were collected by seine net within the frame of pondfishing, in october (pond horné obdokovce--pond a) and december (pond budmerice--pond b) 2003. concentrations of selected metals were measured using an atomic absorption spectropho ...200616760086
use of the p,p'-ddd: p,p'-dde concentration ratio to trace contaminant migration from a hazardous waste site.for approximately 50 years, beginning in the 1920s, hazardous wastes were disposed in an 11-hectare area of the marine corps base (mcb) quantico, virginia, usa known as the old landfill. polychlorinated biphenyls (pcbs) and ddt compounds were the primary contaminants of concern. these contaminants migrated into the sediments of a 78-hectare area of the potomac river, the quantico embayment. fish tissue contamination resulted in the mcb posting signs along the embayment shoreline warning fisherme ...200616758288
pre-operative coronary revascularization: an optimal therapy for high-risk vascular surgery patients?cardiac complications are the leading cause of peri-operative morbidity and mortality of patients undergoing vascular surgery. this high incidence of cardiac complications is related to the presence of underlying coronary artery disease. the optimal treatment strategy for these high-risk patients, including the use of pre-operative coronary revascularization for the purpose of improving peri-operative and long-term cardiac outcomes, has been controversial for several decades. recently, the resul ...200616879464
genomic organization of nucleolin gene in carp fish: evidence for several genes.the protein nucleolin, functionally involved in the main steps of ribosome biogenesis, is codified by a single copy gene in mammals. here we report that at least three different genes codify for this protein in carp fish (cyprinus carpio). this is the first description of the genomic organization of nucleolin in a teleost. the carp nucleolin gene includes 8.8 kb and contains 16 exons. promoter cis regulatory elements are similar to constitutive genes, i.e., a putative tata box, three g/c boxes, ...200616874410
short-term dynamic change of gill copper in common carp, cyprinus carpio, evaluated by a sequential extraction.dynamic changes in cu speciation and its binding to fish gills were investigated by exposing common carp (cyprinus carpio) to a 1 mg/1 cu solution for 7 hours. cu speciation in the bulk solution and fish gill microenvironment was calculated using general chemical equilibrium modeling. a sequential extraction procedure using distilled water, magnesium dichloride (1.0 mol/1), and acetic acid (10%) was used to characterize the cu associated with the fish gills. cu residual in the gill tissue was me ...200616865604
fish otolith asymmetry: morphometry and modeling.mathematical modeling suggests that relatively large values of otolith mass asymmetry in fishes can alter acoustic functionality and may be responsible for abnormal fish behavior when subjected to weightlessness during parabolic or space flight [d.v. lychakov, y.t. rebane, otolith mass asymmetry in 18 species of fish and pigeon, j. grav. physiol. 11 (3) (2004) 17-34; d.v. lychakov, y.t. rebane, fish otolith mass asymmetry: morphometry and influence on acoustic functionality, hear. res. 201 (2005 ...200616859847
effect of deltamethrin on the biochemical profile of common carp (cyprinus carpio l.). 200616855906
behavioral responses of cyprinus carpio to industrial effluents.fish were collected from nath sagar, paithan 50 kms away from aurangabad and were brought to the laboratory and exposed to industrial effluent (tannery effluent). behavioral changes and responses of the fish to the tannery effluent were observed. control fish showed normal behaviour and swimming in contrast the effluent exposed fish showed abnormal swimming, loss of equilibrium, fading of colour, coughing and opercular movements.200616850896
surgical removal of an ovarian tumour in a koi carp (cyprinus carpio).ultrasonography, radiography and exploratory coeliotomy were used to diagnose and treat a large intracoelomic neoplasm from a female koi carp (cyprinus carpio) presented for abdominal enlargement of several months duration. feed was withheld for 1 week immediately prior to surgery and the fish was sedated with isoeugenol (aqui-s) at a dose rate of 10 ml/l to facilitate diagnostic imaging techniques. surgical anaesthesia was induced by adding tricaine (ms-222) 50 mg/l to the water and an explorat ...200616739528
indispensable amino acid concentrations decrease in tissues of stomachless fish, common carp in response to free amino acid- or peptide-based diets.the premise that free amino acid or dipeptide based diets will resolve the nutritional inadequacy of formulated feeds for larval and juvenile fish and improve utilization of nitrogen in comparison to protein-based diets was tested in stomachless fish, common carp (cyprinus carpio l.) larvae. we examined the postprandial whole body free amino acid (faa) pool in fish that were offered a faa mixture based diet for the duration of 2 or 4 weeks. we found that the total amount and all indispensable am ...200616733614
post-genomic approaches to understanding the mechanisms of environmentally induced phenotypic plasticity.post-genomic techniques offer new and detailed insights into the mechanisms underpinning all biological processes, including phenotypic plasticity and environmentally relevant phenotypes. although they require access to genomic resources it is now possible to create these for species of comparative or environmental interest even within a modest research project. here we describe an open transcript screen for genes responding to environmental cold that might account for the acquired cold-specific ...200616731809
induction of gynogenesis in japanese crucian carp (carassius cuvieri).diploid gynogenesis was induced in japanese crucian carp (carassius cuvieri) eggs using uv-irradiated genetically inactive spermatozoa from mirror carp (cyprinus carpio l.) or blunt snout bream (megalobrama amblycephala), with or without cold shock. the optimal radiation dosage was 4,200 mj/cm2 and 3,600 mj/cm2 for mirror carp and blunt snout bream sperm, respectively. at this dosage and without cold shock, the yields were (32.4 +/- 3.3)% vs. (33.8 +/- 1.4)% gynogenetic haploids and (0.7 +/- 0.3 ...200616722335
detection of toxic effects of cd2+ on different fish species via liver cytochrome p450-dependent monooxygenase activities and ftir spectroscopy.the in vivo and in vitro effects of cd2+ and the cyp1a inductor beta-naphthoflavone(beta-nf) on the hepatic cytochrome p450 (cyt 450) monooxygenases were studied in silver carp (hypophthalmichtys molitrix v.), wels (silurus glanis l.), and carp (cyprinus carpio). in vivo treatment of carp with a high dose of cd2+ (10 mg kg(-1), for 3 days) caused a strong inhibition of 7-ethoxyresorufin-o-deethylase (erod) and a lower inhibition of 7-ethoxycoumarin-o-deethylase (ecod) activity. the low-dose cadm ...200616715285
activation of a sensorimotor pathway in response to a water temperature drop in a teleost fish.when common carp, cyprinus carpio l., experience a rapid temperature drop, the cerebral blood volume is strongly reduced to dampen the temperature drop in the brain. simultaneously, the preoptic area and pituitary gland are activated to launch whole-body adaptive responses. however, the preferred reaction of fish to a temperature change is an escape reaction, which implies activation of a sensorimotor pathway. here, we used blood oxygenation level-dependent (bold)- and cerebral blood volume (cbv ...200616709904
[bioaccumulation of titanium dioxide nanoparticles in carp].analysis method for tio2 nanoparticles in water and fish samples was set up using icp-aes after dissolved by solution of sulphuric acid and ammonium sulphate. meanwhile bioaccumulation of tio2 nanoparticles was assessed by tests exposing carp (cyprinus carpio)to tio2 nanoparticles suspensions. the relative standard deviation (rsd) for water sample of 20.0 mg/l tio2 is 4.53%, and the recoveries of tio2 nanoparticles in water and fish samples are raging from 94% - 104% and 90% - 103%, which could ...200617111625
[population genetic variation and structure analysis on five populations of mirror carp cyprinus carpio l. using microsatellites].in this paper, population genetic variability and genetic structure of five populations of an important cultivation species, mirror carp (cyprinus carpio l.) were analyzed using 30 microsatellite loci. the observed (ho) and expected (he) heterozygosity values, polymorphic information content (pic) and number of effective alleles (ae) were all determined. the genetic similarity coefficient and nei's standard genetic distance were computed based on the allele frequencies. the hardy-weinberg equili ...200617138540
experimental hyperthyroidism and central mediators of stress axis and thyroid axis activity in common carp (cyprinus carpio l.).the effect of experimental hyperthyroidism, realized by t(4) injection, on central mediators of the hypothalamo-pituitary-interrenal axis (hpi-axis) in common carp (cyprinus carpio l.) was studied. our results show that hyperthyroidism evokes a marked 3.2-fold reduction in basal plasma cortisol levels. corticotropin-releasing hormone-binding protein (crh-bp) mrna levels in the hypothalamus, measured by real-time quantitative pcr, were significantly elevated by 40%, but crh, urotensin-i, prepro-t ...200617170085
effects of cypermethrin on some haematological, biochemical and histopathological parameters of common carp (cyprinus carpio l.).the study aimed at assessing the effects of a pyrethroid cypermethrin on common carp (cyprinus carpio l.) on the basis of the results of acute toxicity tests and haematological, biochemical and histological examinations.200617159788
primary and secondary sensory trigeminal projections in a cyprinid teleost, carp (cyprinus carpio).primary and secondary sensory trigeminal projections were studied by means of tract-tracing methods in a cyprinid teleost, the carp. tracer injections into the trigeminal nerve root labeled terminals in the ipsilateral principal sensory trigeminal nucleus, descending trigeminal nucleus, medial funicular nucleus, facial lobe, and medial part of posterior lateral valvular nucleus. the principal sensory trigeminal nucleus is considered a major origin of the secondary sensory trigeminal projections ...200617029257
[genetic diversity and differentiation of russian common carp (cyprinus carpio l.) breeds inferred from rapd markers].polymorphic components of the common carp cyprinus carpio l. genome were examined by means of polymerase chain reaction with random primers (rapd-pcr). using four primers, genetic diversity estimates were obtained for 12 populations and seven strains of russian common carp breeds, as well as for european hungarian common carp and amur wild common carp (n = 87). the highest number of polymorphic loci was revealed in angelinskii common carp, as well as in the samples of altai common carp and amur ...200617025163
patterns of gene expression in carp liver after exposure to a mixture of waterborne and dietary cadmium using a custom-made microarray.gene expression changes in carp liver tissue were studied after acute (3 and 24h) and subchronic (7 and 28 days) exposure to a mixture of waterborne (9, 105 and 480 microg/l) and dietary (9.5, 122 and 144 microg/g) cadmium, using a custom-made microarray. suppression subtractive hybridization-pcr (ssh-pcr) was applied to isolate a set of 643 liver genes, involved in multiple biological pathways, such as energy metabolism (e.g. glucokinase), immune response (e.g. complement c3) and stress and det ...200617023062
effects of chronic cysteamine treatment on growth enhancement and insulin-like growth factor i and ii mrna levels in common carp tissues.insulin-like growth factors (igf) belong to a family of growth factors with structural homology to proinsulin. in our previous studies, we found that both igf-i and igf-ii gene expression showed growth hormone (gh) dependence in the brain and liver of juvenile common carp when treated in vivo with gh for a short time. this present work aimed to study the effects of both the short-term and long-term gh induction of igf gene expression using cysteamine (csh) and fasting/re-feeding. csh is an agent ...200617010223
comparison of pah and nonylphenol uptake by carp (cyprinus carpio) and semipermeable membrane devices (spmds) from water. 200616977522
influence of heavy metals and 4-nonylphenol on reproductive function in fish.many industrial and agricultural chemicals (including heavy metals and alkylphenols) present in the environment have adverse effects on the reproductive function in fish. three studies were conducted to assess toxicity of these chemicals towards reproduction of freshwater fish. it was shown that heavy metals added to the diets accumulate in brain tissue of carp, and this accumulation results in inhibition of the secretion of noradrenaline and stimulation of the secretion of dopamine in the hypot ...200616967097
thrombophilia and recurrent pregnancy loss.many unanswered questions regarding thrombophilia and recurrent pregnancy loss exist. for example, does a true association exist? are thrombotic mechanisms relevant? is a second messenger necessary to cause the manifestation of thrombosis? at present it seems that thrombophilia are associated with and may even cause some cases of pregnancy loss. the role of treatment remains to be determined. although the aim of physicians working in this field is entirely laudable, to allow childless couples to ...200616962919
the acute effects of microcystin lr on the transcription of nine glutathione s-transferase genes in common carp cyprinus carpio l.the glutathione s-transferases play important roles in the detoxification of microcystin. in this experiment, nine glutathione s-transferase genes including cytosolic gsts (rho, mu, theta, alpha and pi), mitochondrial gst (kappa) and microsomal gsts (mgst1, mgst2 and mgst3) were cloned from common carp cyprinus carpio. the mrna abundance of each carp gst isoform in liver was analyzed by real time pcr. the relative changes after stimulation with microcystin lr were also analyzed: increased levels ...200617084466
correlations of nonylphenol-ethoxylates and nonylphenol with biomarkers of reproductive function in carp (cyprinus carpio) from the cuyahoga river.various chemical and biological measures were determined in carp (cyprinus carpio) sampled from seven sites along the cuyahoga river, ohio; from the relatively pristine headwaters to the lower portion heavily polluted from various industrial, urban and wastewater treatment plants (wwtp). levels of nonylphenol (np), nonylphenol ethoxylates (npes; np1eo, np2eo) and total npes (np plus the npes) in fish increased in a downstream direction, with maximal values observed below the discharge of the akr ...200617055032
mercury concentrations in fish from lake meredith, texas: implications for the issuance of fish consumption advisories.we examined how length of fish is related to mercury concentrations in muscle tissue of seven species of fish from lake meredith, texas and determined how sex and growth rate are related to mercury concentration in walleye (sander vitreus). flathead catfish (pylodictis olivaris), walleye and white bass (morone chrysops) had the highest concentrations of mercury and channel catfish (ictalurus punctatus), common carp (cyprinus carpio), river carpsucker (carpiodes carpio) and gizzard shad (dorosoma ...200617054010
the interference of pharmaceuticals with endogenous and xenobiotic metabolizing enzymes in carp liver: an in-vitro study.the interactions of fibrate (clofibrate, fenofibrate, bezafibrate, gemfibrozil), antiinflammatory (ibuprofen, diclofenac, naproxen, ketoprofen), and anti-depressive (fluoxetine,fluvoxamine, paroxetine) drugs with cyp catalyzed pathways (cyp1a, cyp3a-, cyp2k-, and cyp2m-like) and phase ii activities (udp-glucuronosyltransferases and sulfotransferases), involved in both xenobiotic and endogenous metabolism in fish, were investigated in-vitro by incubating carp liver subcellular fractions in the pr ...200616955921
gill and kidney histopathology in the freshwater fish cyprinus carpio after acute exposure to deltamethrin.the histopathological effects of deltamethrin on the gill and kidney tissues of the common carp, cyprinus carpio were determined by light microscopy. the fish were exposed to 0.029mgl(-1) (50% of 96h lc(50)) and 0.041mgl(-1) (70% of 96h lc(50)) solutions of deltamethrin for short-term (96h). the most common gill changes at all doses of deltamethrin were desquamation and necrosis. besides, aneurism in secondary lamellae, lifting of the lamellar epithelium, oedema, epithelial hyperplasia and fusio ...200621783710
ulcerative skin disease in a group of koi carp (cyprinus carpio).a case report detailing the presenting clinical signs, diagnostics, and treatment of ulcerative skin disease in a group of koi carp (cyprinus carpio) is presented.200616931397
in vivo and in vitro debromination of decabromodiphenyl ether (bde 209) by juvenile rainbow trout and common carp.decabromodiphenyl ether (bde 209), the major congener in the high volume industrial flame retardant mixture "decabde", has recently been shown to be metabolized by carp. to further explore this phenomenon, juvenile rainbow trout were exposed to bde 209 via the diet for a five month period. analysis of the whole body homogenate, liver, serum, and intestinal tissues revealed that bde 209 accumulated in rainbow trout tissues and was most concentrated in the liver. in addition to bde 209, several he ...200616913120
commentary on "the coronary artery revascularization prophylaxis (carp) trial: results and remaining controversies". 200617351188
the coronary artery revascularization prophylaxis (carp) trial: results and remaining controversies.a high percentage of patients requiring elective vascular surgery also has indications for coronary artery revascularization, leading to varied opinions as to whether to undertake coronary artery revascularization first, to use risk stratification, or to do no coronary intervention before elective vascular surgery. the coronary artery revascularization prophylaxis (carp) trial determined the long-term benefit of coronary artery revascularization in patients with stable coronary artery disease un ...200617351187
nickel induced histopathological changes in the different tissues of freshwater fish, hypophthalmichthys molitrix (valenciennes).nickel chloride, heavy metal widely used in industries was investigated in the present study for histopathological studies in silver carp (hypophthalmichthys molitrix). fish were exposed for 10, 20 and 30 days in sublethal concentration of nickel 5.7 mg/l. the histopathological changes were studied in the gill, liver, intestine and kidney of the nickel treated freshwater fish h. molitrix. the nickel showed a tissue specific alteration in the tissues. mucus proliferation, fusion of the gill lamel ...200617436530
effect of phenol on the metabolism and biochemical composition of the freshwater fish cyprinus carpio.the effect of phenol on the fish cyprinus carpio and the metabolic changes in oxygen consumption and total carbohydrate, total protein and total lipids in the tissues of liver, gills, muscle, brain, and intestine were investigated for sublethal exposure. the oxygen consumption decreased significantly in both toxicants exposed to acute toxicity. following sublethal exposure to phenol total protein, total carbohydrate, and total lipids in the tissues of liver, gills, muscle, brain and intestine of ...200617338283
comparative genomics in cyprinids: common carp ests help the annotation of the zebrafish genome.automatic annotation of sequenced eukaryotic genomes integrates a combination of methodologies such as ab-initio methods and alignment of homologous genes and/or proteins. for example, annotation of the zebrafish genome within ensembl relies heavily on available cdna and protein sequences from two distantly related fish species and other vertebrates that have diverged several hundred million years ago. the scarcity of genomic information from other cyprinids provides the impetus to leverage est ...200617254304
microarray studies of gene expression in fish.the use of microarrays for the study of various aspects of fish physiology has seen a spectacular increase in recent years. from early studies with model species, such as zebrafish, to current studies with commercially important species, such as salmonids, catfish, carp, and flatfish, microarray technology has emerged as a key tool for understanding developmental processes as well as basic physiology. in addition, microarrays are being applied to the fields of ecotoxicology and nutrigenomics. a ...200617233558
behavioral alterations in juvenile cyprinus carpio (linnaeus, 1758) exposed to sublethal waterborne cadmium. 200617219316
dietary branched-chain amino acid valine, isoleucine and leucine requirements of fingerling indian major carp, cirrhinus mrigala (hamilton).three 8-week growth experiments were conducted to quantify the requirements of the fingerling cirrhinus mrigala for the dietary branched-chain amino acids valine (experiment 1), isoleucine (experiment 2) and leucine (experiment 3). six isonitrogenous (400 g/kg) and isoenergetic (17.90 kj/g) test diets were formulated with a gradation of 2.5 g/kg for each test amino acid, valine (7.5-20 g/kg), isoleucine (5.0-17.5 g/kg) and leucine (7.5-20 g/kg), and fed to randomly stocked fish in circular troug ...200616925849
changes in nitrogen metabolism of the freshwater fish cirrhinus mrigala following exposure to cypermethrin.we studied the effect of a synthetic pyrethroid insecticide, cypermethrin, on some aspects of nitrogen metabolism in the gills, liver, and muscle tissue of the indian major carp, cirrhinus mrigala. free amino acid levels and protease activity showed an elevation in all tissues following exposure to lethal (5.13 microg/l) and sublethal (1.026 microg/l) doses of cypermethrin, with significant changes noticed on day 4. the normal condition was restored only at a sublethal concentration.200616639881
reproduction phase-related expression of beta-endorphin-like immunoreactivity in the nucleus lateralis tuberis of the female indian major carp cirrhinus mrigala: correlation with the luteinising hormone cells-ovary axis.the present study aimed to determine whether beta-endorphin immunoreactivity (bep-ir) in the neurones of the nucleus lateralis tuberis (nlt) is linked to the seasonal cycle and shows correlation with the number of luteinising hormone (lh) cells in the pituitary gland and ovaries in the teleost, cirrhinus mrigala. although lh cells were moderately immunostained during the resting phase (december to january), the morphological profile suggested increased synthetic and secretory activity during the ...200616629830
changes in sperm motility in response to osmolality/ca2+ in three indonesian fresh water teleosts: goby (oxyeleotris marmorata), java carp (puntius javanicus), and catfish (clarias batrachus).sperm of most fresh water teleosts become motile when released into the hypotonic fresh water environment, but the role of osmolality and ca2+ on sperm motility is not clear. osmotic pressure and ca2+ concentrations increase from fresh water to brackish water. java carp puntius javanicus and catfish clarias batrachus live and reproduce only in fresh water. on the other hand, goby oxyeleotris marmorata can acclimate and reproduce from fresh water to brackish water. in the present study, sperm mot ...200616458554
isozymes in androgenetic and gynogenetic white amur, gynogenetic carp, and carp-amur hybrids.gynogentic and androgenetic progeny appeared in crosses between white amur, ctenopharyngodon idella, and carp, cyprinus carpio. hemoglobin, plasma general proteins, and plasma isoenzymes were studied by electrophoresis to determine inheritance patterns. electrophoretic bands indicated that gynogenetic white amur had no paternal inheritance from carp and that adrogenetic white amur also were pure white amur. gynogeneiss in carp was confirmed by the absence of paternal inheritance. hemoglobins, ge ...2006939912
purification and molecular cloning of a dna adp-ribosylating protein, carp-1, from the edible clam meretrix lamarckii.the cabbage butterflies pieris rapae and pieris brassicae have unique enzymes, named pierisin-1 and -2, respectively, that catalyze the adp-ribosylation of guanine residues of dna, which has been linked with induction of apoptosis and mutation in mammalian cell lines. in the present study, we identified adp-ribosylation activity targeting dna in six kinds of edible clam. similar to our observations with pierisin-1 and -2, crude extracts from the clams meretrix lamarckii, ruditapes philippinarum, ...200616945908
seasonal changes in glycogen content and na+-k+-atpase activity in the brain of crucian carp.changes in the number of na+-k+-atpase alpha-subunits, na+-k+-atpase activity and glycogen content of the crucian carp (carassius carassius) brain were examined to elucidate relative roles of energy demand and supply in adaptation to seasonal anoxia. fish were collected monthly around the year from the wild for immediate laboratory assays. equilibrium dissociation constant and hill coefficient of [3h]ouabain binding to brain homogenates were 12.87+/-2.86 nm and -1.18+/-0.07 in june and 11.93+/-2 ...200616741142
development of elisa for metallothionein-ii allows determination of heavy metal pollution of fresh water.metallothionein (mt), a metal-binding protein induced primarily by heavy metals in vertebrates, is considered a biomarker for environmental heavy-metal contamination. to investigate heavy metal pollution in the freshwater environment, mt-i and mt-ii were purified from livers of crucian carp (carassius carassius) by gel exclusion chromatography and ion exchange chromatography. to detect the purified mt-ii, a specific monoclonal antibody (mab) against crucian carp mt-ii was produced from the hybri ...200616720298
interactions between predator- and diet-induced phenotypic changes in body shape of crucian carp.predator cues and diet, when studied separately, have been shown to affect body shape of organisms. previous studies show that the morphological responses to predator absence/presence and diet may be similar, and hence could confound the interpretation of the causes of morphological differences found between groups of individuals. in this study, we simultaneously examined the effect of these two factors on body shape and performance in crucian carp in a laboratory experiment. crucian carp (caras ...200616615209
temperature regulates hypoxia-inducible factor-1 (hif-1) in a poikilothermic vertebrate, crucian carp (carassius carassius).hypoxia-inducible transcription factor-1 (hif-1) is a master regulator of hypoxia-induced gene responses. to find out whether hif-1 function is involved in gene expression changes associated with temperature acclimation as well as in hypoxia adaptation in poikilotherms, we studied hif-1 dna binding activity and hif-1alpha expression in normoxia and during hypoxia (0.7 mg l(-1) o2) in crucian carp at temperatures of 26, 18 and 8 degrees c. temperature had a marked influence on hif-1 in normoxia. ...200616513925
gender distinction in neural discrimination of sex pheromones in the olfactory bulb of crucian carp, carassius carassius.studies on projection of the sensory neurons onto the olfactory bulb in fish have revealed a clear subdivision into spatially different areas that each responded specifically to different classes of odorants. amino acids induce activity in the lateral part, bile salts induce activity in the medial part, and alarm substances induce activity in the posterior part of the medial olfactory bulb. in the present study, we demonstrate a new feature of the bulbar chemotopy showing that neurons specifical ...200616322086
specific projection of the sensory crypt cells in the olfactory system in crucian carp, carassius carassius.to study the projection of a special type of sensory neuron called crypt cells in the olfactory system in crucian carp, carassius carassius, we applied the neural tracer 1,1-dilinoleyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (dii) in the olfactory bulb (ob). small crystals of dii were applied in a small area at the synaptic region at the ventral part of the ob, where a population of secondary neurons specific for sex pheromones has been identified. in those samples (4 out of 24) where ...200616306315
distribution characteristics of polychlorinated biphenyls in crucian carp (carassius auratus) from major rivers in korea.polychlorinated biphenyls (pcbs) levels in crucian carp were determined at 20 locations along four major river systems, several small-scale rivers and a wetland in korea. twenty-eight congeners, ranging from tri- to hepta-cbs were detected. a gas chromatograph with a mass selective detector was used to quantify the individual pcb congeners. the objectives of this study were to investigate the levels of contamination of pcbs in freshwater fish and to observe the pattern of their distribution. the ...200615964051
the effect of aromatase inhibitor, fadrozole, on sgnrha stimulated lh secretion in goldfish (carassius auratus) and common carp (cyprinus carpio).the aromatase inhibitor, fadrozole, was applied to common carp and goldfish in order to examine its ability to increase the spontaneous and sgnrha stimulated lh secretion. first, trials in goldfish in 2003 showed fadrozole's moderate ability to potentiate sgnrha-stimulated lh secretion. however, this ability was much weaker than that obtained with dopamine antagonist, pimozide. there was no ovulation in fadrozole-treated fish. several experiments on goldfish and carp during the two consecutive y ...200616967099
gene expression profiling in the neuroendocrine brain of male goldfish (carassius auratus) exposed to 17alpha-ethinylestradiol.17-alpha ethinylestradiol (ee2), a pharmaceutical estrogen, is detectable in water systems worldwide. although studies report on the effects of xenoestrogens in tissues such as liver and gonad, few studies to date have investigated the effects of ee2 in the vertebrate brain at a large scale. the purpose of this study was to develop a goldfish brain-enriched cdna array and use this in conjunction with a mixed tissue carp microarray to study the genomic response to ee2 in the brain. gonad-intact m ...200616954407
endocrine and milt responses of male crucian carp (carassius carassius l.) to periovulatory females under field conditions.laboratory studies in domesticated goldfish (carassius auratus) show that, during the preovulatory luteinizing hormone (lh) surge, females release a complex steroidal pheromone that induces in males a rapid increase of plasma lh, which in turn increases strippable milt (sperm and seminal fluid) prior to ovulation and spawning. the objective of this study was to determine if the same phenomenon occurs in a wild congener, the crucian carp (carassius carassius), under field conditions where fish ar ...200616934255
dietary exposure of mink (mustela vison) to fish from the housatonic river, berkshire county, massachusetts, usa: effects on organ weights and histology and hepatic concentrations of polychlorinated biphenyls and 2,3,7,8-tetrachlorodibenzo-p-dioxin toxic equivalence.the effects of feeding ranch mink (mustela vison) diets containing polychlorinated biphenyl (pcb)-contaminated fish (88 gold fish [carassius auratus] weighing a total of 70.3 kg and 16 carp [cyprinus carpio] weighing a total of 77.3 kg) collected from the housatonic river (hr; berkshire county, ma, usa) in october 1999 on organ weights and histology and hepatic concentrations of total pcbs (sigmapcbs) and 2,3,7,8-tetrachlorodibenzo-p-dioxin toxic equivalence (teq) were evaluated. diets contained ...200616764472
interaction mechanism between cd2+ ions and dna from the kidney of the silver crucian carp.cadmium is one of the most toxic heavy metals and is known to accumulate in freshwater food chains. the underlying mechanism for its genotoxicity has not been investigated for any freshwater fish. it has, however, been suggested that cadmium-induced carcinogenesis might involve either direct or indirect interaction of cd2+ with dna. the interaction between cd2+ and dna from the kidney of the silver crucian carp (carassius auratus gibelio) in vitro and in vivo is investigated by spectrophotometri ...200616679546
evidence of different ploidy eggs produced by diploid f2 hybrids of carassius auratus (female) x cyprinus carpio (male).based on the presence of three types of eggs with different diameters 0.13, 0.17 and 0.2 cm, we made two crosses: f2 (female) x diploid red crucian carp (male), and f2 (female) x f10 tetraploid (male). the ploidy levels of the progeny of the two crosses were examined by chromosome counting and dna content measurement by flow cytometer. in the offspring of the former cross, tetraploids, triploids, and diploid were obtained. in the progeny of the latter cross, tetraploids and triploids were observ ...200616625828
plasticity of respiratory structures--adaptive remodeling of fish gills induced by ambient oxygen and temperature.while a large surface area combined with short diffusion distances make fish gills well suited for gas exchange, these properties leads to costly water and ion fluxes and exposure to toxic substances and pathogens. thus, gill morphology is likely to be a compromise between opposing demands. it has become clear that some fishes have the ability to modify gill structure in response to environmental parameters such as oxygen levels and temperature. maybe the most dramatic example of gill plasticity ...200616540380
demonstration of hematopoietic stem cells in ginbuna carp (carassius auratus langsdorfii) kidney.a model system of ginbuna carp (clonal triploid fish, carassius auratus langsdorfii) and ginbuna-goldfish hybrids (tetraploid fish, having three sets of chromosomes from a triploid clone and a haploid set of chromosomes from goldfish) was employed to demonstrate the presence of hematopoietic stem cells (hscs) in kidney hematopoietic tissues. kidney hematopoietic cells were obtained from ginbuna carp (s3n, donor) and injected into a ginbuna-gold fish hybrid (s4n, recipient) via the caudal sinus. ...200616519942
regulative mechanism of ce3+ relieves dna damage caused by cd2+ in the kidney of silver crucian carp.the mechanism of cd2+ on the dna cleavage and ce3+ on the dna repair in the kidney of silver crucian carp (carassius auratus gibelio) is investigated by agarose gel electrophoresis methods and assaying biochemical indexes. it proves that cd2+ induces the classical laddering degradation of dna in vivo, but dna cleavage is repaired after injecting with a low ce3+ concentration under various cd2+ concentrations. the dna cleavage caused by cd2+ is the result of the activation of deoxyribonuclease (d ...200617194924
comparative studies on histological and ultra-structure of the pituitary of different ploidy level fishes.the histological and ultra-structure of the pituitary in diploid red crucian carp (carassius auratus red var.), triploid crucian carp and allotetraploid hybrids within and after the breeding season were comparatively studied. the result showed that there were six endocrine cell types in the pituitary of these three kinds of fishes, and there was an obvious difference in cell size among different ploidy level fishes. as for the same type of pituitary cells, the cell size was increased gradually w ...200617172051
the graft-versus-host reaction (gvhr) in the ginbuna crucian carp, carassius auratus langsdorfii.a model system of clonal triploid ginbuna and tetraploid ginbuna-goldfish hybrids was employed to demonstrate the presence of graft-versus-host reaction (gvhr) in a teleost fish. tetraploid scale grafts on triploid clone members evoked an acute rejection, whereas the reverse transplants were accepted. when sensitized triploid cells were injected into tetraploid recipients, a typical gvhr was induced, leading to death of the recipients within one month. the onset of illness appeared about one wee ...200610220065
the gut-associated lymphoid tissue (galt) of carp (cyprinus carpio l.): an immunocytochemical analysis.the galt of carp was studied with monoclonal antibodies reacting with carp ig or carp leukocytes, using (dual) immunofluorescence or immunogold staining on cryosections, cytocentrifuge slides, and cell suspensions of the intestine. the intestinal epithelium contained many ig-negative lymphoid cells and, in the hindgut, also many large ig-positive macrophages, which appeared to bind ig. the lamina propria contained numerous ig-positive lymphoid cells next to ig-negative lymphoid cells and granulo ...20068449251
[experimental studies of the effect of rhabdovirus infection on hematological, immunological and biochemical indicators in cold-blooded animals].bactericidal activity of blood serum (babc), content of lysozyme, protein in it were recorded as well as formula and form of erythrocytes, activity of digestive enzymes by results of free amino acid accumulation in the food substrate were determined ten days after rhabdovirus injection to one-year-old carp. it is shown that if there are no clinical symptoms of viremia in carps, babc, relative number of stab neutrophils and the value of eccentricity index in erythrocytes increase. simultaneously ...20062055316
skeletal maturity in belgian youths assessed by the tanner-whitehouse method (tw2).reference data for skeletal maturity (tw2 method) of the hand and wrist are provided for large representative samples of belgian boys and girls. the sample of belgian boys consisted of 21,174 boys aged 12 to 20 years studied in a nationwide cross-sectional and longitudinal study on the physical fitness of secondary schoolboys (1969-1974). the girls' sample consisted of 9698 6-19-year-old flemish girls studied cross-sectionally (1979-1980). both samples were multi-stage stratified cluster samples ...20062260839
urotensin i and its n-terminal flanking peptide from the flounder, platichthys flesus.the caudal spinal cord region of teleost fish terminates in a neurosecretory organ, the urophysis. two peptides have been purified to homogeneity from an extract of the urophysis of a teleost fish, the flounder. the primary structure of one peptide, ser-glu-asp-pro-pro-met-ser-ile-asp-leu10-thr-phe-his-met-leu-arg- asn-met-ile- his20-met-ala-lys-met-glu-gly-glu-arg-glu-gln30-ala-gln-ile- asn-arg-asn-leu-leu - asp-glu40-val, indicates identity with urotensin i. by analogy with other urotensins, t ...20062284199
ecological aspects of nematode parasites of introduced salmonids from valdivia river basin, chile.between 1986 and 1987, 666 fishes distributed among the following species introduced in chile, and from different sectors of the valdivia river basin (39 degrees 30'-40 degrees 00's, 73 degrees 30'-71 degrees 45'w), were examined: 348 salmo trutta, 242 salmo gairdneri, 24 cyprinus carpio and 52 gambusia affinis holbrooki. the presence of camallanus corderoi and contracaecum sp. in s. gairdneri and of c. corderoi in s. trutta is recorded in chile for the first time. cyprinus carpio and g. a. holb ...20061842391
[electric parameters of the l-type horizontal cell layer in the carp retina].the inner longitudinal resistance of the l-type horizontal cell syncytium in the carp retina ranges from 1.5 x 10(5) to 7,4 x 10(5) ohm. the static resistance of the nonsynaptic membrane at the potential level corresponding to the bright light ranges from 2,5 x 10(3) to 19 x 10(3) ohm . cm2 i.e. comparable with membrane resistances of many neurons recently investigated. depolarization increases the resistance in 3,5 divided by 21 times up to 16 x 10(3) divided by 69.10(3) ohm . cm2. measured val ...20066639967
[the electroretinogram of the dark-adapted intact carp carpio cyprinus l. to color substitution].electroretinogram b-wave was studied in intact carp using instantaneous substitution of monochromatic stimulus for another one in the range of 404-681 nm. it was shown that the amplitude of the b-wave as a function of the wavelength was good approximated by the spectrum of a scotopic pigment (porphiropsin) with the absorbtion maximum near 530 nm. the amplitude of the b-wave recorded during alternation of different monochromatic stimuli was determined only by their intensity differences and the l ...20067754686
[transfer of the pronator teres tendon to the extensor carp radialis tendon in wrist flexion deformity in cerebral palsy].the flexion deformity of the wrist in cerebral palsy is quite common, carrying disability to the effective use of the hands. from 1980 to 1990, 36 transferences of the pronator teres to the extensor carpi radialis were reviewed. the authors describe the operative technique by a unique incision, that may be used in cerebral palsy, and conclude that it is a good method for correction of deformity.20067817107
detection of mhc class ii transcripts in lymphoid tissues of the common carp (cyprinus carpio l.).in all vertebrates studied to date, the expression of mhc class ii genes is known to be restricted to a limited number of tissues and cell types. in order to have a better understanding of the function of the equivalent genes in teleost fish, the distribution of mhc class ii beta transcripts (cyca-dab) in the common carp (cyprinus carpio l.) was investigated. rna was isolated from tissues and leucocytes, cdna was produced, and amplification of the cyca-dab genes was carried out by pcr. of the or ...20068801130
[the perceptive brightness space in the carp (carpio cyprinus l.)].brightness discrimination of black-white (in the range of 0.0082-0.214 w/m2; cie chromaticity coordinates x = 0.340, y = 0.354) and red (in the range of 0.0035-0.106 w/m2; cie chromaticity coordinates x = 0.641, y = 0.342) stimuli was studied during using choice conditioning paradigm in two carps. confusion matrices for black-white and red stimuli separately were constructed from probabilities of instrumental responses (catching of a bead) to conditioned and differential stimuli in sequential si ...20068560943
studies on the structure and function of the carp gonadotropin alpha subunit by site-directed mutagenesis.there are two genes encoding the alpha subunit of carp gonadotropin (alpha 1 and alpha 2 subunits). our previous data have demonstrated that both alpha 1 and alpha 2 subunits expressed in insect cells are able to associate with the beta subunit, but only the alpha 1/beta heterodimer displays biological activity. in the mature protein, there are only four amino-acid residues different between the alpha 1 and alpha 2 subunits. in this study we used site-directed mutagenesis and expressed different ...20068537173
regional distribution of vasoactive intestinal peptide immunoreactivity in the brain of salmon, trout and carp.the content of vasoactive intestinal peptide (vip) was determined by radioimmunoassay in different brain regions of three fish species, namely salmon, trout and carp. the vip extracted from salmon brain was shown by reverse-phase high-performance liquid chromatography to coelute with native porcine vip. highest vip levels were measured in the salmon brain with a maximum of 112 pg/mg protein in the hypothalamus, followed by the telencephalon, olfactory bulb and optic tectum. in contrast, in the t ...20068590917
expression of mhccyca class i and class ii molecules in the early life history of the common carp (cyprinus carpio l.)in this study transcription of class i alpha chain (cyca-ua), beta2-microglobulin (cyca-b2m) and class ii alpha (cyca-dxa) and beta (cyca-dab) during the early stages of embryo development was investigated by semiquantitative pcr. no transcripts of the genes under investigation were detected in the unfertilized egg. the expression of the genes encoding for the class ii molecules revealed to be synchronized starting at day 1, unlike those for the class i molecules. transcription of cyca-b2m was f ...20069877432
ontogeny of the thymus in a teleost fish, cyprinus carpio l.: developing thymocytes in the epithelial microenvironment.a monoclonal antibody, wcl9, specific for membrane molecules of a thymocyte subpopulation was used to detect these cells in situ during the ontogeny of thymus. cryo-sections revealed wcl9+ cells in the rudiment of the thymus (day 4 post fertilization); thereafter, the positive cells were observed exclusively in the cortex from the first appearance of thymic regionalization (week 4 post fertilization) until adult age. whole-mount immunostaining of the thymus with wcl9 revealed the three-dimension ...200610227480
conservation of an alpha 2 domain within the teleostean world, mhc class i from the rainbow trout oncorhynchus mykiss.a full-length cdna clone (onmy-ua-c32) encoding a major histocompatibility complex (mhc) class i heavy chain was isolated from a rainbow trout thymus cdna library. onmy-ua-c32 alpha i and iii extracellular domains were most similar to other salmonids (92 and 86% at the nucleotide and amino acid level) but interestingly the alpha ii domain is closer to that of the carp (74 and 73%) and zebrafish (75 and 70%). in addition, onmy-ua-c32 displays conservation of residues known to be essential for the ...20069040984
[nadph-diaphorase in the olfactory system of the carp cyprinus carpio]. 200611966211
improved erythrocyte lysis assay in microtitre plates for sensitive detection and efficient measurement of haemolytic compounds from ichthyotoxic algae.haemolytic substances produced by ichthyotoxic algae often are unknown in molecular structure or specific mechanism of toxicity. detection and quantification of such substances are dependent on bioassays, using markers that are sensitive for haemolytic impairment and generation of a recordable response. the erythrocyte lysis assay (ela) represents an advantageous bioassay in this respect, because the lytic response can be measured photometrically by the amount of released haemoglobin. the aim of ...200611746200
[nucleocytoplasmic incompatibility in androgenetic fish hybrids can be overcome].many androgenetic fish hybrids are nonviable due to the disturbed interaction between the foreign nucleus and the cytoplasm. it has been proposed that this incompatibility can be overcome if eggs from interspecific hybrids and sperm from one of the parental species are used for diploid androgenesis. indeed, the androgenetic progeny obtained as a result of the insemination of the enucleated eggs of goldfish and carp hybrids by the carp spermatozoa proved to be viable and fertile. this approach ma ...200611605409
[the budget model of ecosystem of a shallow highly eutrophic lake].a model of energy budget of lake bolshoi okunenok ecosystem was based on the data received during field studies from may through november 1986. the model takes into account 36 components including dissolved organic matter, bacteria, phytoplankton, zooplankton, meiobenthos, macrobenthos, fish, suspended and sediment detritus. the growing season has been divided into 16 intervals according to the number of observations. the balance equation for each live component describes the change in its bioma ...200612723370
ultrastructure of carp cyprinus carpio permatozoa after cooling, dilution and freeze-thawing.dilution of the carp spermatozoa with a cryoprotective medium was shown to inflict serious injuries to the fine structures of some spermatozoa. the amount of the injured spermatozoa increases after cryopreservation. examination of the freeze-fracture micrographs of the heads of the carp spermatozoa, showed that their protoplasmic faces were covered with the particles in high concentrations. these particles do not form clusters after cooling the spermatozoa down to +5 degrees c and dilution with ...200612644853
the energy metabolism of common carp (cyprinus carpio) when exposed to salt stress: an increase in energy expenditure or effects of starvation?stenohaline common carp (cyprinus carpio) were chronically exposed to the two main osmoregulatory ions, na+ and cl-, at levels close to their isoosmotic value for 28 d (171 mm nacl; 324 mosm kg-1; 10 per thousand). the aim of this study was to assess whether or not the disturbed ion and osmoregulation affected the energy demand and the energy stores of the exposed fish. salt exposure reduced food intake by 70% and had adverse effects on growth and survival. although food consumption decreased an ...200610685912
[quantitative and species composition of yeasts in carp and trout grown in the discharges from the kiev thermoelectric power plant]. 20067329344
[absence of parvalbumins in the muscles of lampetra fluviatilis lampreys].comparative data on isolation and identification of parvalbumins from muscles of the lamprey lampetra fluviatilis and carp cyprinus carpio are presented. no parvalbumins or some other soluble low molecular weight sarcoplasmic calcium-binding proteins were detected in the lamprey, while 4 pure fractions of parvalbumins were obtained from carp muscles. these data imply that cyclostomes do not need additional calcium regulation of the activity of fast skeletal muscles by parvalbumins.20067386089
[characteristics of summary electrical responses to sound in the acoustico-lateral region of the medulla oblongata of cyrpinus carpio carp]. 2006941578
[distribution of monoamines in the hypothalamus and hypophysis of carp, cyprinus carpio].monoaminergic fibres exhibiting green fluorescence have been revealed by the falck histochemical fluorescence technique in the area of the hypothalamic preoptic nuclei and nucleus lateralis tuberis of the carp. monoaminergic cerebrospinal fluid-contacting nerve cells with an intensive greenish and yellowish fluorescence were observed in the walls of the lateral and posterior recesses of the third ventricle. monoaminergic fibres are few in the pro-and meta-adenohypophysis. yellowish-green fluores ...2006941576
[the effect of pituitary injections on the isoenzyme composition of non-specific esterases in the tissues of the silver carp, hypophthalmichthys molitrix].polyacrylamide gel electrophoresis reveals differences in the isoenzymic spectra of non-specific esterases from various tissues of h. molitrix. hypophyseal injections result in changes of the isoenzymic spectra of all the tissues studied. the pattern of changes depends on the tissue examined. some of the isoenzymes are resistant to hypophyseal injections, whereas the other ones undergo changes which depend on the dose of the hormone.2006941591
[total electrical response to sound in the acoustico-lateral region of the carp, cyrpinus carpio, medulla oblongata].in order to investigate foci of the electrical activity, studies have been made on the topography and form of total reactions to sound in the acoustico-lateral part of the medulla oblongata in the carp c. carpio. two foci of the activity were found -- medical and ventral ones. responses in the medial and ventral foci differ with respect to the form, latency (2.0--2.5 and 1.3--1.5 msec. respectively) and amplitude (200--600 and 50--200 microvolts respectively). it is suggested that total reaction ...2006983552
[correlative growth of body weight and brain in carp fry]. 2006729524
gill sphaerosporosis in the common carp and grasscarp. 2006112845
[action potentials of the heart atria and ventricles of rana temporaria frogs and cyprinus carpio carp].using microelectrode technique, studies have been made on electrophysiological indices (amplitude of ap, amplitude of the plateau, latent period of ap, duration of maximal depolarization, duration of repolarization at different levels) of cells of isolated atrium and ventricles of the carp during both the spontaneous activity and electrical stimulation. the obtained amplitude-temporal parameters were compared to those of the heart in the frog r. temporaria. it was found that the amplitude of ap, ...20061088024
[several acoustic properties of the carp, cyprinus carpio, swimbladder]. 2006868399
Displaying items 4101 - 4200 of 7554