Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| infective endophthalmitis caused by bacillus cereus after cataract extraction surgery. | we describe a 72-year-old chinese man who developed fulminant bacillus cereus endophthalmitis 36 h after an uneventful surgery for cataract extraction. clinical progression of disease was extremely rapid, in spite of early vitrectomy and intravitreal injection of antibiotics. results of gram staining showed gram-positive bacilli, and the culture was positive for b. cereus. endophthalmitis is rare after cataract surgery (cumulative probability within 1 year, approximately 0.08%), and cases caused ... | 2003 | 12884186 |
| isolation and identification of thiocyanate utilizing chemolithotrophs from gold mine soils. | a mixed bacterial culture capable of growing in potassium-thiocyanate containing medium (200 mg kscn) has been isolated from bacterial suspensions of soil samples collected near gold mines in kumjung (korea). the isolates were initially characterized by metabolic profile analysis and were identified as bacillus thermoglucosidasius, bacillus cereus, bacillus licheniformis, bacillus mycoides, brevibacterium epidermidis, brevibacterium otitidis, and corynebacterium nitrilophilus. one of the seven i ... | 2003 | 12889608 |
| synthesis of a nitrogen analogue of sphingomyelin as a sphingomyelinase inhibitor. | [structure: see text] sphingomyelin nitrogen analogue 1 was designed and synthesized as a sphingomyelinase inhibitor. the synthesis was established by continuous hofmann rearrangement and crutius rearrangement as key steps in constructing the 3-hydroxy-1,2-diamine structure in the backbone of 1. this analogue showed moderate inhibitory activity toward smase isolated from b. cereus. | 2003 | 12889878 |
| diverse effects of ascorbic acid and palmitoyl ascorbate on helicobacter pylori survival and growth. | among many antioxidants used in the food, pharmaceutical and cosmetic industries, ascorbic acid (aa) is one of the most important. aa has been suggested to decrease the risk of gastric disease (gastritis, duodenal ulcer, and carcinoma) by direct action on helicobacter pylori. however, there are limited studies on the possible role of aa and its derivatives such as palmitoyl ascorbate (pa) on the growth and survival of h. pylori. in the present study it was demonstrated in vitro that aa in the co ... | 2003 | 12892889 |
| a constitutively expressed 36 kda exochitinase from bacillus thuringiensis hd-1. | a 36 kda chitinase was purified by ion exchange and gel filtration chromatography from the culture supernatant of bacillus thuringiensis hd-1. the chitinase production was independent of the presence of chitin in the growth medium and was produced even in the presence of glucose. the purified chitinase was active at acidic ph, had an optimal activity at ph 6.5, and showed maximum activity at 65 degrees c. of the various substrates, the enzyme catalyzed the hydrolysis of the disaccharide 4-mu(gln ... | 2003 | 12893268 |
| rebeccamycin analogues bearing amine substituents or other groups on the sugar moiety. | in the course of structure-activity relationship studies on rebeccamycin analogues, a series of compounds bearing an amino function on the sugar moiety were synthesized with the aim of improving the solubility and interaction with the macromolecular target(s). the syntheses of amino derivatives and the corresponding chloro, iodo and azido intermediates are described. their interaction with dna and effects on human dna topoisomerases i and ii were investigated. their antimicrobial activities agai ... | 2003 | 12901916 |
| pcr assay of the groel gene for detection and differentiation of bacillus cereus group cells. | strains of species in the bacillus cereus group are potentially enterotoxic. thus, the detection of all b. cereus group strains is important. as 16s ribosomal dna sequence analysis cannot adequately differentiate species of the b. cereus group, we explored the potential of the groel gene as a phylogenetic marker. a phylogenetic analysis of the groel sequences of 78 b. cereus group strains revealed that the b. cereus group strains were split into two major clusters, one including six b. mycoides ... | 2003 | 12902235 |
| pgil01, a linear tectiviral plasmid prophage originating from bacillus thuringiensis serovar israelensis. | bacillus thuringiensis serovar israelensis harbours, in addition to several circular plasmids, a small linear molecule of about 15 kb. sequence analysis of this molecule, named pgil01, showed the presence of at least 30 orfs, five of which displayed similarity with proteins involved in phage systems: a b-type family dna polymerase, a lexa-like repressor, two potential muramidases and a dna-packaging protein (distantly related to the p9 protein of the tectiviral phage prd1). experimental evidence ... | 2003 | 12904548 |
| screening of bifidobacterium strains isolated from human faeces for antagonistic activities against potentially bacterial pathogens. | as probiotic bacteria, strains belonging to the genus bifidobacterium colonise the gastro-intestinal tract of humans and animals at the time of birth, and they are found in young as well as in adult individuals in great numbers. moreover, they can interact with the development of enteric infections by the production of antimicrobial metabolites. in this work 281 strains of bifidobacteria were anaerobically isolated from human faecal samples, supplied by volunteers of different ages (youngs, adul ... | 2003 | 12906392 |
| rho-specific bacillus cereus adp-ribosyltransferase c3cer cloning and characterization. | c3-like adp-ribosyltransferases represent an expanding family of related exoenzymes, which are produced by clostridia and various staphylococcus aureus strains. here we report on the cloning and biochemical characterization of an adp-ribosyltransferase from bacillus cereus strain 2339. the transferase encompasses 219 amino acids; it has a predicted mass of 25.2 kda and a theoretical isoelectric point of 9.3. to indicate the relationship to the family of c3-like adp-ribosyltransferases, we termed ... | 2003 | 12911311 |
| using x-ray crystallography of the asp55asn mutant of the phosphatidylcholine-preferring phospholipase c from bacillus cereus to support the mechanistic role of asp55 as the general base. | because mutations of the ionizable asp at position 55 of the phosphatidylcholine preferring phospholipase c from bacillus cereus (plc(bc)) to a non-ionizable asn generate a mutant enzyme (d55n) with 10(4)-fold lower catalytic activity than the wild-type enzyme, we tentatively identified asp55 as the general base for the enzymatic reaction. to eliminate the alternate possibility that asp55 is a structurally important amino acid, the x-ray structures of unbound d55n and complexes of d55n with two ... | 2003 | 12921783 |
| intractable hypotension in septic shock: successful treatment with vasopressin in an infant. | 2003 | 12929303 | |
| antioxidant and antimicrobial activity of cissus quadrangularis l. | extracts of cissus quadrangularis l. were tested for antioxidant activity by beta-carotene linoleic acid model and also by 1,1-diphenyl-2-picrylhydrazyl model. the ethyl acetate fraction of both fresh and dry stem extracts at a concentration of 100 ppm showed 64.8% antioxidant activity in the beta-carotene linoleic acid system and 61.6% in the 1,1-diphenyl-2-picrylhydrazyl system. this fraction showed the presence of sterols, vitamin c, and tannins as phytoconstituents. the antioxidant activity ... | 2003 | 12935320 |
| the biodegradation of 1,3-dichlorobenzene by an adapted strain bacillus cereus pf-11 derived from town-gas industrial effluent. | in the present study, an adapted bacterium pf-11 with high 1,3-dichlorobenzene degradation capability was isolated from town-gas industrial effluent through continuous introducing of n-methyl-n'-nitro-n-nitrosoquanidine (ntg). in suitable condition, a degradation rate of 32 mg l(-1) d(-1) of 1,3-dichlorobenzene was obtained by strain pf-11 with effective chlorion release. strain pf-11 was tentatively identified as gram-positive bacillus cereus. the substrate specificity of the strain pf-11 was r ... | 2003 | 12940485 |
| analysis of whole bacterial cells by flow field-flow fractionation and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. | the purpose of this study is to develop a novel bacterial analysis method by coupling the flow field-flow fractionation (flow fff) separation technique with detection by matrix-assisted laser desorption/ionization time-of-flight (maldi-tof) mass spectrometry. the composition of carrier liquid used for flow fff was selected based on retention of bacterial cells and compatibility with the maldi process. the coupling of flow fff and maldi-tof ms was demonstrated for p. putida and e. coli. fractions ... | 2003 | 12948145 |
| surviving the acid test: responses of gram-positive bacteria to low ph. | gram-positive bacteria possess a myriad of acid resistance systems that can help them to overcome the challenge posed by different acidic environments. in this review the most common mechanisms are described: i.e., the use of proton pumps, the protection or repair of macromolecules, cell membrane changes, production of alkali, induction of pathways by transcriptional regulators, alteration of metabolism, and the role of cell density and cell signaling. we also discuss the responses of listeria m ... | 2003 | 12966143 |
| design, synthesis, and evaluation of water-soluble phospholipid analogues as inhibitors of phospholipase c from bacillus cereus. | the rate of hydrolysis of natural phospholipids by the phosphatidylcholine-preferring phospholipase c from bacillus cereus (plc(bc)) follows the order phosphatidylcholine > phosphatidylethanolamine >> phosphatidyl-l-serine. to probe the structural basis for this substrate specificity, a series of water-soluble, nonhydrolyzable substrate analogues were needed so their complexes with the enzyme could be studied via x-ray crystallography and isothermal titration calorimetry (itc). accordingly the w ... | 2003 | 12968879 |
| application of sonication to release dna from bacillus cereus for quantitative detection by real-time pcr. | a rapid sonication method for lysis of gram-positive bacteria was evaluated for use in combination with quantitative real-time polymerase chain reaction (pcr) analyses for detection. other criteria used for evaluation of lysis were microscopic cell count, colony forming units (cfu), optical density at 600 nm and total yield of dna measured by picogreen fluorescence. the aim of this study was complete disruption of cellular structures and release of dna without the need for lysing reagents and ti ... | 2003 | 14499990 |
| development of a rapid 1-h fluorescence-based cytotoxicity assay for listeria species. | listeria monocytogenes is cytotoxic to the lymphocyte-origin hybridoma ped-2e9 cell line. the relative cytotoxicity can be calculated by assaying the release of alkaline phosphatase (alp) from the infected cell line. in this study, a fluorogenic substrate (4-methylumbelliferyl phosphate, mup) was used to quantify the alp activity. the assay is 3.5-fold more sensitive than the colorimetric-based assay and requires only 1 h to differentiate virulent from avirulent strains. in addition to various l ... | 2003 | 14499993 |
| interaction of a free-living soil nematode, caenorhabditis elegans, with surrogates of foodborne pathogenic bacteria. | free-living nematodes may harbor, protect, and disperse bacteria, including those ingested and passed in viable form in feces. these nematodes are potential vectors for human pathogens and may play a role in foodborne diseases associated with fruits and vegetables eaten raw. in this study, we evaluated the associations between a free-living soil nematode, caenorhabditis elegans, and escherichia coli, an avirulent strain of salmonella typhimurium, listeria welshimeri, and bacillus cereus. on an a ... | 2003 | 14503703 |
| pseudo-outbreak of toxigenic bacillus cereus isolated from stools of three patients with diarrhoea after oral administration of a probiotic medication. | from december 2000 to january 2001 toxigenic bacillus cereus was isolated from stools of three patients with diarrhoea at two tertiary hospitals in southwest germany. two cases with nosocomial diarrhoea were apparently epidemiologically related (same time and ward), a third case was unrelated with respect to time and location. in order to investigate the epidemiology of these three cases, clinical isolates and isolates from an unexpected, possible common source (probiotic medication) were compar ... | 2003 | 14505607 |
| complexation of cyclic dodecadepsipeptide, cereulide with ammonium salts. | cereulide is a principal toxin causing emetic syndrome which is produced by bacillus cereus and has been known as potassium selective ionophore. this paper deals with its complexation with inorganic and organic ammonium ions to assign the higher structures similar to the complex with potassium ion by means of nmr and esi-ms spectroscopy. of particular interest, the detectable ions are not only at m/z 1191.8 as k(+) complex but also (or sometimes exclusively) at m/z 1170.8 as nh(4)(+) complex in ... | 2003 | 14505659 |
| an antifungal chitinase produced by bacillus cereus with shrimp and crab shell powder as a carbon source. | the production of inexpensive chitinolytic enzymes is an element in the utilization of shellfish processing wastes. in this study, shrimp and crab shell powder prepared by treating shrimp and crab processing wastes with boiling and crushing was used as a substrate for the isolation of an antifungal chitinase-producing microorganism. bacillus cereus yq 308, a strain isolated from the soil samples, excreted one chitinase when cultured in a medium containing 2% (wt/vol) shrimp and crab shell powder ... | 2003 | 14506855 |
| an efficient and selective enzymatic oxidation system for the synthesis of enantiomerically pure d-tert-leucine. | [reaction: see text] d-tert-leucine was prepared with an enantiomeric excess of >99% by an enzyme-catalyzed oxidative resolution of the racemic mixture of dl-tert-leucine with use of leucine dehydrogenase. the l-amino acid was oxidized completely due to coupling of the primary reaction with a highly efficient irreversible nad(+)-regenerating step by nadh oxidase. | 2003 | 14507195 |
| diacylglycerol-rich domain formation in giant stearoyl-oleoyl phosphatidylcholine vesicles driven by phospholipase c activity. | we have studied the effect of phospholipase c from bacillus cereus and clostridium perfringens (alpha-toxin) on giant stearoyl-oleoyl phosphatidylcholine (sopc) vesicles. enzyme activity leads to a binary mixture of sopc and the diacylglycerol sog, which phase separates into a sopc-rich bilayer phase and a sog-rich isotropic bulk-like domain embedded within the membrane, as seen directly by phase contrast microscopy. after prolonged enzymatic attack, all bilayer membranes are transformed into an ... | 2003 | 14507699 |
| antibacterial and proteolytic activity in venom from the endoparasitic wasp pimpla hypochondriaca (hymenoptera: ichneumonidae). | venom from the endoparasitic wasp, pimpla hypochondriaca, is composed of a mixture of high and low molecular weight proteins, possesses phenoloxidase activity, has immunosuppressive properties, and induces paralysis in several insect species. in the present study we demonstrate that p. hypochondriaca venom also contains antibacterial and proteolytic activity. antibacterial activity was detected against the gram-negative bacteria escherichia coli and xanthamonas campestris but not against pseudom ... | 2003 | 14511827 |
| use of bromodeoxyuridine immunocapture to identify active bacteria associated with arbuscular mycorrhizal hyphae. | arbuscular mycorrhizae are beneficial for crops grown under low-till management systems. increasingly, it is becoming apparent that bacteria associated with mycorrhizae can enhance the beneficial relationship between mycorrhizae and plants. however, it has been difficult to study these relationships by conventional techniques. in this study actively growing bacteria were identified in soil from an undisturbed fallow field known to contain arbuscular mycorrhizae by using molecular tools to elimin ... | 2003 | 14532082 |
| use of denaturing high-performance liquid chromatography to identify bacillus anthracis by analysis of the 16s-23s rrna interspacer region and gyra gene. | denaturing high-performance liquid chromatography (dhplc) was evaluated as a method for identifying bacillus anthracis by analyzing two chromosomal targets, the 16s-23s intergenic spacer region (isr) and the gyra gene. the 16s-23s isr was analyzed by this method with 42 strains of b. anthracis, 36 strains of bacillus cereus, and 12 strains of bacillus thuringiensis; the gyra gene was analyzed by this method with 33 strains of b. anthracis, 27 strains of b. cereus, and 9 strains of b. thuringiens ... | 2003 | 14532217 |
| a new multiplex pcr for the detection of hbl genes in strains of the 'bacillus cereus group'. | 2003 | 14535496 | |
| morphogenesis of bacillus spore surfaces. | spores produced by bacilli are encased in a proteinaceous multilayered coat and, in some species (including bacillus anthracis), further surrounded by a glycoprotein-containing exosporium. to characterize bacillus spore surface morphology and to identify proteins that direct formation of coat surface features, we used atomic-force microscopy (afm) to image the surfaces of wild-type and mutant spores of bacillus subtilis, as well as the spore surfaces of bacillus cereus 569 and the sterne strain ... | 2003 | 14563859 |
| microbial ecology of bacillus thuringiensis: fecal populations recovered from wildlife in korea. | a total of 34 fecal samples, collected from 14 species of wild mammals in korea, were examined for the occurrence of bacillus thuringiensis. the organism was detected in 18 (53%) samples. among the three food-habit groups, herbivorous animals yielded the highest frequency (69%) of samples positive for b. thuringiensis, followed by omnivorous animals (50%). of the six fecal samples from carnivorous animals, only one sample contained b. thurin giensis. among 527 isolates belonging to the bacillus ... | 2003 | 14569287 |
| bactericidal effects of konjac fluid on several food-poisoning bacteria. | in this study, the bactericidal effects of japanese alkaline foods on food-poisoning bacteria were evaluated. konjac is an alkaline food soaked in calcinated calcium (the ph of konjac fluid ranges from 11.42 to 12.53). konjac fluids completely inactivated escherichia coli, enterohemorrhagic e. coli o157:h7 and e. coil o26:h9, salmonella enteritidis, vibrio parahemolyticus. and staphylococcus aureus. the initial level of 6 log cfu/ml dramatically decreased after incubation with konjac fluid, and ... | 2003 | 14572219 |
| consumer food-handling behaviors associated with prevention of 13 foodborne illnesses. | to be effective in reducing the incidence of foodborne illness, consumers and food safety educators need information about behaviors that will decrease exposure to foodborne pathogens. a four-round delphi technique was used to survey nationally recognized experts in food microbiology, epidemiology, food safety education, and food safety policy with the aim of identifying and ranking food-handling and consumption behaviors associated with 13 major foodborne pathogens. the food safety experts rank ... | 2003 | 14572229 |
| phosphatidylcholine-specific phospholipase c and sphingomyelinase activities in bacteria of the bacillus cereus group. | bacillus anthracis is nonhemolytic, even though it is closely related to the highly hemolytic bacillus cereus. hemolysis by b. cereus results largely from the action of phosphatidylcholine-specific phospholipase c (pc-plc) and sphingomyelinase (sph), encoded by the plc and sph genes, respectively. in b. cereus, these genes are organized in an operon regulated by the global regulator plcr. b. anthracis contains a highly similar cereolysin operon, but it is transcriptionally silent because the b. ... | 2003 | 14573681 |
| ceramide is involved in r(+)-methanandamide-induced cyclooxygenase-2 expression in human neuroglioma cells. | cannabinoids have recently been shown to induce the expression of the cyclooxygenase-2 (cox-2) isoenzyme in h4 human neuroglioma cells. using this cell line, the present study investigates the contribution of the second messenger ceramide to this signaling pathway. incubation of cells with the endocannabinoid analog r(+)-methanandamide (r(+)-ma) was associated with an increase of intracellular ceramide levels. enhancement of ceramide formation by r(+)-ma was abolished by fumonisin b1, a ceramide ... | 2003 | 14573769 |
| microbial transformation of cortisol and prolyl endopeptidase inhibitory activity of its transformed products. | incubation of cortisol (1) with gibberella fujikuruoi for 12 days yielded an oxidatively cleaved product, 11beta-hydroxyandrost-4-en-3,17-dione (2), while incubation with bacillus subtilis and rhizopus stolonifer yielded the reduced product, 11beta, 17alpha,20,21-tetrahydroxy-(20s)-pregn-4-en-3-one (3). other reduced products, 11beta, 17alpha, 21-trihydroxy-5alpha-pregnan-3, 20-dione (4) and 3beta, 11beta, 17alpha, 21-tetrahydroxy-5alpha-pregnan-20-one (5) were obtained by incubation of compound ... | 2003 | 14577687 |
| real-time molecular beacon nasba reveals hblc expression from bacillus spp. in milk. | nucleic acid sequence-based amplification (nasba) was applied in combination with a fluorescein-conjugated molecular beacon specific for a sequence flanked by transcript-specific primers in order to monitor hblc enterotoxin gene expression in real-time from milk separately contaminated with bacillus amyloliquefaciens, bacillus cereus, and bacillus circulans. maximal enterotoxin expression was noted following 16, 15, and 16 h, respectively, when grown in artificially contaminated nonfat dried mil ... | 2003 | 14592426 |
| aerobic tryptophan degradation pathway in bacteria: novel kynurenine formamidase. | while a variety of chemical transformations related to the aerobic degradation of l-tryptophan (kynurenine pathway), and most of the genes and corresponding enzymes involved therein have been predominantly characterized in eukaryotes, relatively little was known about this pathway in bacteria. using genome comparative analysis techniques we have predicted the existence of the three-step pathway of aerobic l-tryptophan degradation to anthranilate (anthranilate pathway) in several bacteria. based ... | 2003 | 14592712 |
| inactivation of spores of bacillus cereus in cheese by high hydrostatic pressure with the addition of nisin or lysozyme. | the objective of this work was to study high hydrostatic pressure (hhp) inactivation of spores of bacillus cereus atcc 9139 inoculated in model cheeses made of raw milk, together with the effects of the addition of nisin or lysozyme. the concentration of spores in model cheeses was approximately 6-log10 cfu/g of cheese. cheeses were vacuum packed and stored at 8 degrees c. all samples except controls were submitted to a germination cycle of 60 mpa at 30 degrees c for 210 min, to a vegetative cel ... | 2003 | 14594224 |
| wessex shopping basket survey - a structured approach to local food sampling. | the shopping basket approach to food sampling, in which ready-to-eat food items from a shopping list were sampled at premises chosen at random, was adopted by 15 environmental health departments in the wessex region. a total of 2037 samples were analysed over a 30-month period. the microbiological quality varied considerably between food categories, with gravy and stock samples giving the highest proportion of satisfactory results. sliced meats, cooked rice and sandwiches gave the poorest overal ... | 2003 | 14594701 |
| composition and antimicrobial activity of achillea clavennae l. essential oil. | the volatile constituents of achillea clavennae l. (asteraceae), rare plant of europe, have been analysed using gc/ms. twenty- five components making up 81.6% of the oil were characterized with camphor (29.5%), myrcene (5.5%), 1,8-cineole (5.3%), beta-caryophyllene (5.1%) and linalool (4.9%) being the major constituents. the essential oil was evaluated for antibacterial and antifungal activities. the screening of the antimicrobial activity of essential oil was conducted by a disc diffusion test ... | 2003 | 14595583 |
| microbial diversity and prevalence of virulent pathogens in biofilms developed in a water reclamation system. | bacterial biofilm is a common phenomenon in both natural and engineered systems which often becomes a source of contamination and microbially influenced corrosion. it is thought that formation of biofilm in the monoculture of several bacterial species is regulated by acylhomoserine lactone (ahl) quorum-sensing signals. in this study, we investigated the microbial diversity and existence of ahl-producing and ahl-degrading bacterial species in the biofilm samples from a water reclamation system lo ... | 2003 | 14596899 |
| microbial and copper adsorption by smectitic clay--an experimental study. | the objective of this study was to quantify copper-, bacteria- and bacteriophage-binding capacities of natural clay with the aim of predicting the adsorption of heavy metals, human pathogenic bacteria and viruses by a clayey landfill liner. x-ray diffraction analysis of six natural clays showed that the dominant phase in all deposits consists of smectites together with illite, kaolin and, sometimes, palygorskite and sepiolite. the specific surface areas of different clay substrates were very hig ... | 2003 | 14599145 |
| in vitro assay for human toxicity of cereulide, the emetic mitochondrial toxin produced by food poisoning bacillus cereus. | the in vitro boar spermatozoon test was compared with the lc ion trap ms analysis for measuring the cereulide content of a pasta dish, implemented in serious emetic food poisoning caused by bacillus cereus. both assays showed that the poisonous food contained approximately 1.6 microg of cereulide g(-1) implying the toxic dose in human as < or =8 microg kg(-1) body weight. the threshold concentration of cereulide provoking visible mitochondrial damage in boar sperm exposed in vitro was 2 ng of ce ... | 2003 | 14599471 |
| identification of genomic islands in the genome of bacillus cereus by comparative analysis with bacillus anthracis. | horizontal gene transfer has been recognized as a universal event throughout bacterial evolution. the availability of both complete genome sequences of bacillus cereus and b. anthracis provides the possibility to perform comparative analysis based on their genomes. by using a windowless method to display the distribution of the genomic gc content of b. cereus and b. anthracis, we have found three genomic islands in the genome of b. cereus, i.e., bcgi-1, bcgi-2, and bcgi-3, respectively, which ar ... | 2003 | 14600214 |
| duplex real-time sybr green pcr assays for detection of 17 species of food- or waterborne pathogens in stools. | a duplex real-time sybr green lightcycler pcr (lc-pcr) assay with dna extraction using the qiaamp dna stool mini kit was evaluated with regard to detection of 8 of 17 species of food- or waterborne pathogens in five stool specimens in 2 h or less. the protocol used the same lc-pcr with 20 pairs of specific primers. the products formed were identified based on a melting point temperature (t(m)) curve analysis. the 17 species of food- or waterborne pathogens examined were enteroinvasive escherichi ... | 2003 | 14605150 |
| identification of foodborne bacteria by infrared spectroscopy using cellular fatty acid methyl esters. | identification of bacterial species by profiling fatty acid methyl esters (fames) has commonly been carried out by using a 20-min capillary gas chromatographic procedure followed by library matching of fame profiles using commercial midi databases and proprietary pattern recognition software. fast gc (5 min) fame procedures and mass spectrometric methodologies that require no lipid separation have also been reported. in this study, bacterial identification based on the rapid (2 min) infrared mea ... | 2003 | 14607413 |
| interaction of phospholipases c and sphingomyelinase with liposomes. | 2003 | 14610804 | |
| genes for an alkaline d-stereospecific endopeptidase and its homolog are located in tandem on bacillus cereus genome. | alkaline d-peptidase (adp) from bacillus cereus df4-b is a d-stereospecific endopeptidase acting on oligopeptides composed of d-phenylalanine and the primary structure deduced from its gene, adp, shows a similarity with d-stereospecific hydrolases from ochrobactrum anthropi strains. we have isolated dna fragments covering the flanking region of adp from df4-b genome and found an additional gene, adp2, located upstream of adp. the deduced amino acid sequence of adp2 showed 96% and 85% identity wi ... | 2003 | 14612229 |
| nosocomial bacteremia and catheter infection by bacillus cereus in an immunocompetent patient. | we present a case of bacillus cereus bacteremia and catheter infection in an immunocompetent patient subjected to abdominal surgery, who recovered following central catheter removal and treatment with piperacillin/tazobactam. | 2003 | 14616690 |
| the bacillus thuringiensis linear double-stranded dna phage bam35, which is highly similar to the bacillus cereus linear plasmid pbclin15, has a prophage state. | bam35, a 15-kbp double-stranded dna phage, infects bacillus thuringiensis. recently, sequencing of the related bacillus cereus revealed a 15.1-kbp linear plasmid, pbclin15. we show that pbclin15 closely resembles bam35 and demonstrate conversion of bam35 to a prophage. this state is common, as several b. thuringiensis strains release bam35-related viruses. | 2003 | 14617663 |
| inhibition of bacillus cereus by strains of lactobacillus and lactococcus in milk. | the growth and death or survival of bacillus cereus in sterile skimmed milk fermented with 18 different lactic acid bacteria (lab) were investigated. b. cereus alone in milk reached about 10(7)-10(8) colony-forming units (cfu)/ml. when b. cereus was cultivated together with different lactobacillus or lactococcus cultures at 30 or 37 degrees c, the b. cereus counts after 72 h of fermentation ranged between < 10 cfu/ml and about 10(6) cfu/ml. the inhibition patterns for the different lactobacillus ... | 2003 | 14623386 |
| effect of gamma irradiation on the survival of pathogens in kwamegi, a traditional korean semidried seafood. | kwamegi (semidried raw pacific saury) is traditional seafood available in korea. it has water activity in the range of 0.90 to 0.95. spoilage and the growth of most pathogenic bacteria is retarded because of low water activity, low temperature, and packaging. however, it is contaminated with bacteria of public health significance and poses a hazard to the consumer because it is consumed raw without any cooking. the effectiveness of these hurdles in preventing the growth of staphylococcus aureus, ... | 2003 | 14627288 |
| a bacillus thuringiensis isolate possessing a spore-associated filament. | we report on a novel bacterium, isolated during a screen for environmental isolates of bacillus thuringiensis, that possesses a novel filamentous structure. nucleotide sequence from the isolate's 16s rrna gene places the bacterium unambiguously within the bacillus thuringiensis/bacillus cereus group. phase-contrast and electron microscopy indicate the presence of both a parasporal body and a long filament which are retained after sporulation. the filament is shown to consistently arise from the ... | 2003 | 14629020 |
| rapid determination of vitamin b2 secretion by bacteria growing on solid media. | development of an agar-diffusion assay to measure vitamin b2 in biological samples and application of the method to determine the amount of vitamin b2 secreted by bacteria. | 2003 | 14632999 |
| a molecular method to detect bacillus cereus from a coffee concentrate sample used in industrial preparations. | the aim of this work was to develop specific primers which are able to detect bacillus cereus in a coffee concentrate sample. | 2003 | 14633011 |
| detection and characterization of the novel bacteriocin entomocin 9, and safety evaluation of its producer, bacillus thuringiensis ssp. entomocidus hd9. | to identify and characterize new bacteriocins from a collection of 41 strains belonging to 27 subspecies of bacillus thuringiensis, and to evaluate the safety of the producers. | 2003 | 14633027 |
| genehunter, a transposon tool for identification and isolation of cryptic antibiotic resistance genes. | genehunter is a transposon tool designed for the experimental activation and identification of silent antibiotic resistance genes. the method permits the identification of novel resistance genes that lack previously identified homologues. using salmonella enterica serovar typhimurium strain lt2 as a test organism for the in vivo version of the genehunter method, we were able to activate, clone, and identify two cryptic antibiotic resistance genes, the aminoglycoside acetyltransferase aac(6')-iaa ... | 2003 | 14638492 |
| lethal gastroenteritis associated with clozapine and loperamide. | 2003 | 14638602 | |
| antiplasmid effect of promethazine in mixed bacterial cultures. | promethazine has been recognised as an effective antiplasmid agent in cultures containing a single bacterial species such as escherichia coli, yersinia enterocolitica, staphylococcus aureus and agrobacterium tumefaciens. the objective of this study was to examine the effect of heterogenity of the microbial flora on plasmid elimination by promethazine in a laboratory based model system of mixed bacterial infection. f'lac plasmid elimination of e. coli k12 le140 was studied in the presence of a nu ... | 2003 | 13678824 |
| essential oil analysis and antimicrobial activity of eight stachys species from greece. | the volatile composition of eight stachys species has been studied. the investigated taxa were st. alopecuros (l.) bentham., st. scardica (griseb.) hayek, st. cretica l. ssp. cretica, st. germanica l. ssp. heldreichii (boiss.) hayek, st. recta l., st. spinulosa l., st. euboica rech. and st. menthifolia vis., growing wild in greece. the essential oils were obtained by hydrodistillation in a modified clevenger-type apparatus, and their analyses were performed by gc and gc-ms. identification of the ... | 2003 | 13679097 |
| finding optimal photosensitisers for the decontamination of foods by the photodynamic effect. | a new method for sanitation based on the photodynamic effect is introduced. photosensitisers are compounds that generate cytotoxic oxygen species and free radicals after excitation with appropriate visible light. the cell killing efficacy is due to their chemical structure, to the actual environment and therefore also to their degree of aggregation. as has been shown, photosensitisers bound to the cell wall or incorporated into the cells are very effective cytotoxic agents after illumination. ev ... | 2003 | 15806940 |
| [organic extracts with antimicrobian activity from penicillium sp. (moniliales) isolated from the sponge ircinia felix (porifera: demospongiae)]. | this research expresses the potential of the bacterial activity present in the organic extracts obtained from penicillium sp., isolated from the esponge irciniafelix. this activity was evaluated through agar diffusion test and minimal inhibitory concentration (mic). the susceptibility trials of organic fractions were carried out against staphylococcus aureus, s. epidermidis, bacillus cereus and b. subtilis. the use of the chromatographic techniques (clv and tlc), permitted to obtain bioactive or ... | 2003 | 15264564 |
| antibacterial activity of the fourth-generation fluoroquinolones gatifloxacin and moxifloxacin against ocular pathogens. | the ideal ophthalmic anti-infective exhibits broad-spectrum activity against gram-positive, gram-negative, and atypical bacterial species. these pathogens can cause potentially blinding infections such as keratitis and endophthalmitis, both of which are associated with ophthalmic surgery or traumatic injury. these infections often require aggressive antibacterial therapy, preferably with newer generations of antibiotics. in this study, minimal inhibitory concentration (mic) values for gatifloxac ... | 2003 | 14964344 |
| synthesis of 6-fluoro-1,4-dihydro-4-oxo-quinoline-3-carboxylic acid derivatives as potential antimicrobial agents. | in the present study, a series of 1-ethyl/benzyl-6-fluoro-7-(substituted piperazin-1-yl)1,4-dihydro-4-oxo-quinoline-3-carboxylic acid were synthesized and characterized by ir, 1h-nmr, mass spectral and elemental analysis. the in vitro antibacterial and antifungal activities of the compounds were evaluated by paper disc diffusion method. the minimum inhibitory concentrations (mic) of the compounds were also determined by agar streak dilution method. the in vivo antibacterial activity of the compo ... | 2003 | 14642332 |
| antibacterial activity and mechanism of action of tick defensin against gram-positive bacteria. | defensins are a major group of antimicrobial peptides and are found widely in vertebrates, invertebrates and plants. invertebrate defensins have been identified from insects, scorpions, mussels and ticks. in this study, chemically synthesized tick defensin was used to further investigate the activity spectrum and mode of action of natural tick defensin. synthetic tick defensin showed antibacterial activity against many gram-positive bacteria but not gram-negative bacteria and low hemolytic activ ... | 2003 | 14642822 |
| conformational changes induced by cloxacillin in class a beta-lactamase from bacillus cereus. | class a beta-lactamases are enzymes that hydrolyse beta-lactam antibiotics such as penicillins and cephalosporins. they also hydrolyse substrate analogues such as oxacillin and cloxacillin, with a biphasic kinetic as it has been reported for bacillus cereus beta-lactamase. a molecular model of bacillus cereus beta-lactamase was built and the conformational changes that the substrates benzylpenicillin and cloxacilline produced in the conformation of selected regions of the protein were analyzed. ... | 2003 | 14656057 |
| fatal bacillus cereus meningitis without inflammatory reaction in cerebral spinal fluid after bone marrow transplantation. | 2003 | 14657704 | |
| inactivation of geobacillus stearothermophilus spores by high-pressure carbon dioxide treatment. | high-pressure co2 treatment has been studied as a promising method for inactivating bacterial spores. in the present study, we compared this method with other sterilization techniques, including heat and pressure treatment. spores of bacillus coagulans, bacillus subtilis, bacillus cereus, bacillus licheniformis, and geobacillus stearothermophilus were subjected to co2 treatment at 30 mpa and 35 degrees c, to high-hydrostatic-pressure treatment at 200 mpa and 65 degrees c, or to heat treatment at ... | 2003 | 14660357 |
| asymmetric reduction of benzil to (s)-benzoin with whole cells of bacillus cereus. | benzil (1) was selectively reduced to (s)-benzoin (2) in the presence of a wild-type bacillus cereus tim-r01. a 92% yield of 2 with 94% enantiomeric excess ratio was attained in phosphate-buffered saline (pbs) (ph 7.5) by using glucose as a nutrient at 37 degrees c for 12 h. compound 2 was not reduced further to hydrobenzoin (3) at all. the reduction activity differed greatly depending on the strain of b. cereus. under these conditions the b. cereus strains ifo3001, ifo15305, iam1110, iam1229, i ... | 2003 | 14665737 |
| cytotoxicity and antimicrobial activity of goniothalamin isolated from bryonopsis laciniosa. | the cytotoxicity and antimicrobial activity of goniothalamin isolated from bryonopsis laciniosa was investigated. goniothalamin showed potent cytotoxicity with lc50 values (5.03 microg/ml) comparable with the reference standard agent, gallic acid. it also showed weak antibacterial and significant antifungal activity against a wide range of gram positive and gram negative bacteria and fungi. the antibacterial (minimum inhibitory concentration) effect against bacillus cereus and shigella shiga was ... | 2003 | 14669248 |
| treatment of saline wastewaters from marine-products processing factories by activated sludge reactor. | an activated sludge reactor, operated at room temperature (20-30 degrees c) was used to treat saline wastewaters generated by marine-products industries. the system was operated continuously and the influence of the organic loading rates (olrs), varying from 250 to 1000 mg cod l(-1) day(-1), on chemical oxygen demand (cod) removal was investigated. the system, inoculated with nacl-acclimated culture, removed up to 98% and 88% of the influent cod concentrations at olrs of 250 and 1000 mg cod l(-1 ... | 2003 | 14669806 |
| expression of binary toxin genes in the mosquito-colonizable bacteria, bacillus cereus, leads to high toxicity against culex quinquefasciatus larvae. | two b. cereus strains, ae10 and cx5, isolated from mosquito larval guts, were transformed with a recombinant plasmid, pbs373, harboring binary toxin genes from bacillus sphaericus 2297. immunoblotting analysis clearly revealed the production and presence of the 51-kda toxin protein in both strains. two recombinant b. cereus strains ae10 and cx5 showed very high toxicity against c. quinquefasciatus larvae. since both strains have a close relationship with the mosquito larvae in the native environ ... | 2003 | 14669911 |
| [conjugation in bacilli]. | the review considers experimental data on the conjugal transfer of plasmids in the bacillus cereus and bacillus subtilis groups (the transfer of large self-transmissible plasmids and the mobilization of small plasmids). conjugation in bacilli is compared with conjugation in e. coli dependent on the f factor. conjugation of bacilli in their natural habitats is also discussed. | 2003 | 14679894 |
| liver abscess due to bacillus cereus: a case report. | bacillus cereus is a food-borne pathogen that causes a self-limiting gastroenteritis. we describe the case of a 72-year-old woman admitted to our hospital because of acute abdominal colic pain. over a 2-day period, her clinical condition deteriorated rapidly, with the appearance of acute abdomen. computed tomography investigation of the abdomen showed a liver abscess (diameter approximately 3 cm). at laparotomy, the abscess was found to be ruptured to the free peritoneal cavity. the final clinic ... | 2003 | 14686990 |
| the antimicrobial activity of extracts of the lichen cetraria aculeata and its protolichesterinic acid constituent. | in this study, the antimicrobial activity of the acetone, diethyl ether and ethanol extracts of the lichen cetraria aculeata has been investigated. the extracts were tested against twelve bacteria and eight fungi and found active against escherichia coli, staphylococcus aureus, aeromonas hydrophila, proteus vulgaris, streptococcus faecalis, bacillus cereus, bacillus subtilis, pseudomonas aeruginosa, listeria monocytogenes. no antimicrobial activity against the fungi was detected. it was determin ... | 2003 | 14713164 |
| inactivation of bacillus cereus spores in milk by mild pressure and heat treatments. | 2003 | 14702650 | |
| maldi analysis of bacilli in spore mixtures by applying a quadrupole ion trap time-of-flight tandem mass spectrometer. | a novel ion trap time-of-flight hybrid mass spectrometer (qit-tof ms) has been applied for peptide sequencing in proteolytic digests generated from spore mixtures of bacilli. the method of on-probe solubilization and in situ proteolytic digestion of small, acid-soluble spore proteins has been recently developed in our laboratory, and microorganism identification in less than 20 min was accomplished. in this study, tryptic peptides were generated in situ from complex spore mixtures of b. subtilis ... | 2003 | 14710845 |
| characterization of bacillus spore species and their mixtures using postsource decay with a curved-field reflectron. | a strategy is proposed for the rapid identification of bacillus spores, which relies on the selective release of a family of proteins, referred to as small, acid-soluble spore proteins (sasps). in this work, sasps were selectively solubilized from bacillus spores on the maldi sample plate by using 10% tfa. proteolytic digests of sasps generated in situ from spores of b. subtilis 168, b. globigii, b. thuringiensis subs. kurstaki hd-1, b. cereus t, and the nonpathogenic strain b. anthracis sterne ... | 2003 | 14710846 |
| [microbiological quality of muesli samples purchased in retail network]. | the estimation of microbiological quality of muesli samples was the aim of this investigation. the study included 40 samples which represented various consignments of muesli, produced in 2002 year. total number of aerobic mesophilic bacteria, yeasts and moulds, and also occurrence of pathogenic bacteria, coliforms and enterococci were determined. the systematic units of muesli fungal flora have been identified. it was stated that microbiological quality of all tested muesli was good. no pathogen ... | 2003 | 14755854 |
| the role of bacterial and non-bacterial toxins in the induction of changes in membrane transport: implications for diarrhea. | bacterial toxins induce changes in membrane transport which underlie the loss of electrolyte homeostasis associated with diarrhea. bacterial- and their secreted toxin-types which have been linked with diarrhea include: (a) vibrio cholerae (cholera toxin, e1 tor hemolysin and accessory cholera enterotoxin); (b) escherichia coli (heat stable enterotoxin, heat-labile enterotoxin and colicins); (c) shigella dysenteriae (shiga-toxin); (d) clostridium perfringens (c. perfringens enterotoxin, alpha-tox ... | 2003 | 14757199 |
| [evaluation of a colorimetric method for studying the interaction between microorganisms and intestinal epithelial cells]. | the aim of the present study was to gain further insight on the reliability of the colorimetric determination of the activity of bacterial nitrate reductase to evaluate bacterial concentrations and interaction between microorganisms and enterocyte-like cells. nitrite produced after incubation of the samples with a nitrate-formate solution was determined with a diazotization reaction with sulphanilic acid and n-naphthyl-ethylene-diamonium dichloride. cell association assays were performed with di ... | 2003 | 14976870 |
| antibacterial effects of carica papaya fruit on common wound organisms. | the purpose of the study was to investigate antibacterial activity of ripe and unripe carica papaya on selected micro-organisms. cultures of micro-organisms were routinely maintained in nutrient agar slants at 4 degrees c. extracts of immature, mature and ripe carica papaya fruit were obtained by separately grinding factions of the epicarp, endocarp and seeds and filtering them through gauze. sensitivity tests were conducted by adding 0.06 ml of extract to agar wells (6 mm diameter) prepared fro ... | 2003 | 15040064 |
| a novel cyanobacteriolytic bacterium, bacillus cereus, isolated from a eutrophic lake. | isolation and screening of cyanobacteriolytic bacteria were carried out. fifteen strains of cyano-bacteriolytic bacteria were isolated by the double layer method using the cyanobacterium, microcystis, as a sole nutrient. the isolate, n-14, showing the highest cyanobacteriolytic activity was identified as bacillus cereus based on the 16s rrna sequence. components among the extracellular products in the culture supernatant of b. cereus were responsible for the cyanobacteriolytic activity. lytic as ... | 2003 | 16233388 |
| the inhibitory effect of garlic (allium sativum) on growth of some microorganisms. | the aim of the present study was to compare between the anti-microbial activity of fresh garlic bulbs and that of its water extract against the potent food pathogens staphylococcus aureus, esherichia coil, salmonella typhi and bacillus cereus. garlic segments with different concentrations (1%, 5%, and 10%) were used. another 100 gms of garlic segments were weighed and mixed with 100 ml distilled water to prepare water extract of garlic, then 1000, 500 and 100 microg/ml were prepared. these conce ... | 2003 | 17219900 |
| microbial succession in the rhizosphere of live and decomposing barley roots as affected by the antagonistic strain pseudomonas fluorescens dr54-bn14 or the fungicide imazalil. | abstract the protocol used in the present study was a long-term mesocosm experiment where the microbial succession around live barley roots and subsequent decomposing roots was assessed after seed coating with either the antagonistic strain pseudomonas fluorescens dr54-bn14 or the fungicide imazalil. four diversity measures were used: community level physiological profiles (clpp), bacteria-specific polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge), actinomycete-specifi ... | 2003 | 19719670 |
| sources of bacillus cereus contamination in a pasteurized zucchini purée processing line, differentiated by two pcr-based methods. | in previous work, raw materials used for processing pasteurized zucchini purée (zucchini, milk proteins and starch) and cultivation soil of zucchinis were found to be potential sources of stored product contamination with bacillus cereus. 134 b. cereus strains originating from these sources and from the stored product were typed using coliphage m13 sequence-based polymerase chain reaction (m13-pcr) and dna amplification fingerprinting. combined patterns from the two methods were compared using d ... | 2003 | 19719681 |
| phytochemical and antimicrobial studies of extracts of solanum xanthocarpum. | antibacterial activity pf various parts (stem, leaf and fruits) of solvent extracts (petroleum ether, alcohol and acetone) of solanum xanthocarpum against escherichia coli, klebsiella pneumoniae, salmonella typhi and bacillus cereus were detected by zone of inhibition. the extracts of solanum xanthocarpum showed high sensitivity to kiebsiella pneumoniae and salmonella typhi, moderate sensitivity to escherichia coli and less sensitivity and resistant to bacillus cereus. in control, there is no in ... | 2003 | 22557117 |
| phenolic metabolites of dalea versicolor that enhance antibiotic activity against model pathogenic bacteria. | a new flavonoid (1) was isolated from organic extracts of dalea versicolor, along with six known phenolic compounds (2-7). the structures of the seven compounds were determined by nmr and hrms methods. these compounds were evaluated for direct activity against a variety of organisms in vitro, including the gram-positive bacteria staphylococcus aureus and bacillus cereus. in addition, the compounds were evaluated for their ability to potentiate the activity of known antimicrobials through inhibit ... | 2004 | 15043439 |
| novel inhibition mechanism of bacillus cereus sphingomyelinase by beryllium fluoride. | phosphate analogs have been known to inhibit competitively various phosphatases and phospholipase c and d. we found for the first time that only beryllium fluoride (bef(x)) among the phosphate analogs studied inhibits bacillus cereus sphingomyelinase (smase) activity. the active inhibitory species proved to be not bef(3)(-) but bef(2) by the measurement of smase activity and of (19)f nmr spectroscopy in the presence of a fixed concentration of becl(2) and different concentrations of naf, althoug ... | 2004 | 15047192 |
| circular dichroism of protein-folding intermediates. | 2004 | 15051341 | |
| rational design of a novel fluorescent biosensor for beta-lactam antibiotics from a class a beta-lactamase. | a rational design strategy was used to construct a sensitive "turn-on" biosensor for beta-lactam antibiotics and beta-lactamase inhibitors from a class a beta-lactamase mutant with suppressed hydrolytic activity. a fluorescein molecule was attached to the 166 position on the omega-loop of the e166c mutant close to the active site of the beta-lactamase. upon binding with antibiotics or inhibitors, the flexibility of the omega-loop allows the fluorescein molecule to move out from the active site a ... | 2004 | 15053574 |
| characterization of a major bacillus anthracis spore coat protein and its role in spore inactivation. | a major bacillus anthracis spore coat protein of 13.4 kda, designated cot alpha, was found only in the bacillus cereus group. a stable ca. 30-kda dimer of this protein was also present in spore coat extracts. cot alpha, which is encoded by a monocistronic gene, was first detected late in sporulation, consistent with a sigma(k)-regulated gene. on the basis of immunogold labeling, the protein is in the outer spore coat and absent from the exosporium. in addition, disruption of the gene encoding co ... | 2004 | 15060044 |
| the 26 nudix hydrolases of bacillus cereus, a close relative of bacillus anthracis. | the genome of bacillus cereus contains 26 nudix hydrolase genes, second only to its closest relative, bacillus anthracis which has 30. all 26 genes have been cloned, 25 have been expressed, and 21 produced soluble proteins suitable for analysis. substrates for 16 of the enzymes were identified; these included adp-ribose, diadenosine polyphosphates, sugar nucleotides, and deoxynucleoside triphosphates. one of the enzymes was a cdp-choline pyrophosphatase, the first nudix hydrolase active on this ... | 2004 | 15060060 |
| a napin-like polypeptide from dwarf chinese white cabbage seeds with translation-inhibitory, trypsin-inhibitory, and antibacterial activities. | napins are 1:1 disulfide-linked complexes of a smaller (ca. 4kda) subunit and a larger (ca. 10kda) subunit. the intent of the present study was to ascertain the production of napin by the seeds of a brassica species that has not been examined previously, and also to explore new biological activities of the napin. a heterodimeric 11-kda napin-like polypeptide has been isolated from chinese white cabbage (brassica chinensis cv dwarf) seeds with a protocol comprising ion exchange chromatography on ... | 2004 | 15062997 |
| detection of bacillus cereus group bacteria from cardboard and paper with real-time pcr. | the aim of this study was to develop a pcr-based rapid method to detect bacillus cereus group cells from paper and cardboard. primers targeting the 16s rdna and real-time pcr with sybr green i detection were used in order to be able to also quantify the target. both autoclaved cardboard samples spiked with b. cereus vegetative cells or spores and naturally contaminated paper and cardboard samples were studied. results were compared with culturing verified by commercial (api) tests. several diffe ... | 2004 | 15064974 |
| characterization of bacillus probiotics available for human use. | bacillus species (bacillus cereus, bacillus clausii, bacillus pumilus) carried in five commercial probiotic products consisting of bacterial spores were characterized for potential attributes (colonization, immunostimulation, and antimicrobial activity) that could account for their claimed probiotic properties. three b. cereus strains were shown to persist in the mouse gastrointestinal tract for up to 18 days postadministration, demonstrating that these organisms have some ability to colonize. s ... | 2004 | 15066809 |
| growth and sporulation of bacillus cereus atcc 14579 under defined conditions: temporal expression of genes for key sigma factors. | an airlift fermentor system allowing precise regulation of ph and aeration combined with a chemically defined medium was used to study growth and sporulation of bacillus cereus atcc 14579. sporulation was complete and synchronous. expression of siga, sigb, sigf, and sigg was monitored with real-time reverse transcription-pcr, and the pattern qualitatively resembled that of bacillus subtilis. this method allows reproducible production of stable spores, while the synchronous growth and defined con ... | 2004 | 15066852 |
| shelf life and safety concerns of bakery products--a review. | bakery products are an important part of a balanced diet and, today, a wide variety of such products can be found on supermarket shelves. this includes unsweetened goods (bread, rolls, buns, crumpets, muffins and bagels), sweet goods (pancakes, doughnuts, waffles and cookies) and filled goods (fruit and meat pies, sausage rolls, pastries, sandwiches, cream cakes, pizza and quiche). however, bakery products, like many processed foods, are subject to physical, chemical and microbiological spoilage ... | 2004 | 15077880 |
| cytotoxic bacillus spp. belonging to the b. cereus and b. subtilis groups in norwegian surface waters. | to investigate the presence and numbers of bacillus spp. spores in surface waters and examine isolates belonging to the b. cereus and b. subtilis groups for cytotoxicity, and to discuss the presence of cytotoxic bacillus spp. in surface water as hazard identification in a risk assessment approach in the food industry. | 2004 | 15078515 |