Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
a bivalent vibrio harveyi dna vaccine induces strong protection in japanese flounder (paralichthys olivaceus).vibrio harveyi is a severe aquaculture pathogen that can infect a large number of fish species. in previous studies, we have identified two protective immunogens, degq and vhp1, from pathogenic v. harveyi strains. in this study, we examined the potentials of degq and vhp1 as monovalent and bivalent dna vaccines in a japanese flounder (paralichthys olivaceus) model. for this purpose, the dna vaccine plasmids pdegq, pvhp1, and pdv were constructed. pdegq and pvhp1 express, respectively, degq and v ...201121513763
cloning of a novel collagenase gene from the gram-negative bacterium grimontia (vibrio) hollisae 1706b and its efficient expression in brevibacillus choshinensis.the collagenase gene was cloned from grimontia (vibrio) hollisae 1706b, and its complete nucleotide sequence was determined. nucleotide sequencing showed that the open reading frame was 2,301 bp in length and encoded an 84-kda protein of 767 amino acid residues. the deduced amino acid sequence contains a putative signal sequence and a zinc metalloprotease consensus sequence, the hexxh motif. g. hollisae collagenase showed 60 and 59% amino acid sequence identities to vibrio parahaemolyticus and v ...201121515782
multiplex pcr assay for identification of three major pathogenic vibrio spp., vibrio cholerae, vibrio parahaemolyticus, and vibrio vulnificus.a multiplex pcr assay was developed based on atpa-sequence diversification for molecular identification of 3 major pathogenic vibrio species: vibrio cholerae, vibrio parahaemolyticus, and vibrio vulnificus. it specifically identified them from among 133 strains of various vibrio species and other genera, and was applicable for testing seawater, suggesting its usefulness.201121530641
rapid detection of vibrio parahaemolyticus by pcr targeted to the histone-like nucleoid structure (h-ns) gene and its genetic characterization.aims:  the aim of this study was to explore a new pcr target gene for vibrio parahaemolyticus, based on the histone-like nucleoid structure (h-ns) gene. methods and results:  primers for the h-ns gene were designed for specificity to v. parahaemolyticus, and incorporated into a pcr assay. the pcr assay was able to specifically detect all of the 82 v. parahaemolyticus strains tested, but did not result in amplification in the 47 other vibrio spp. and non-vibrio spp. strains. the detection limit o ...201121535049
identification and evaluation as a dna vaccine candidate of a virulence-associated serine protease from a pathogenic vibrio parahaemolyticus isolate.a putative serine protease gene was cloned from the genomic dna of vibrio parahaemolyticus fyz8621.4. the gene consisted of 1779 base pairs and encoded a 592 amino acid protein. the gene was expressed in escherichia coli. the expressed protease was purified by ni-nta his-bind resin column and showed a 63 kda band on sds-page. the protease exhibited proteolytic activity on gelatin agar plate and showed maximal proteolytic activity at ph 8.0 and 37 °c. it hydrolyzed n-α-benzoyl-l-tyrosine p-nitroa ...201121536140
[experiences in the epidemiological surveillance of foodborne pathogens by pulsed field gel electrophoresis (pfge) in peru.]foodborne diseases and other enteric infections often occur as outbreaks and cause morbidity and mortality all over the world. in perú, they represent a serious public health problem, and are caused by a great variety of infectious agents. for epidemiological research, a wide array of typification methods are used. one of the most important tools for the molecular subtyping of bacterial pathogens is the pulsed field gel electrophoresis (pfge), which is a highly precise method that allows the dis ...201121537781
purification, crystallization and preliminary x-ray crystallographic analysis of the putative vibrio parahaemolyticus resuscitation-promoting factor yeaz.vibrio parahaemolyticus is a human pathogen associated with gastroenteritis caused by the ingestion of contaminated raw seafood. v. parahaemolyticus is able to survive exposure to low temperatures typical of those used for the refrigeration of foods by entering a viable but nonculturable (vbnc) state. the vbnc cells can regain culturability and renewed ability to cause infection upon temperature upshift. the resuscitation-promoting factor protein (rpf, yeaz) plays a key role in reactivation of g ...201121543872
genetic markers of pandemic vibrio parahaemolyticus: are they truly unique? 201121548769
vibrio parahemolyticus septicaemia in a liver transplant patient: a case report.abstract: introduction: vibrio parahemolyticus is the leading cause of vibrio-associated gastroenteritis in the united states of america, usually related to poor food handling; only rarely has it been reported to cause serious infections including sepsis and soft tissue infections. in contrast, vibrio vulnificus is a well-known cause of septicaemia, especially in patients with cirrhosis. we present a patient with v. parahemolyticus sepsis who had an orthotic liver transplant in 2007 and was on i ...201121548914
influence of growth conditions on pressure resistance of vibrio parahaemolyticus in oysters and the optimization of postpressure treatment recovery conditions.vibrio parahaemolyticus atcc 43996 was grown at 15°c for 53 h, 20°c for 24 h, 25°c for 12 h, 30°c for 9 h, 35°c for 9 h, or 40°c for 6 h to early stationary phase. oyster meats were blended, autoclaved at 121°c for 15 min, inoculated with v. parahaemolyticus, and pressure treated at 250 mpa for 2 and 3 min and at 300 mpa for 1 and 2 min at 21°c. overall, growth temperatures of 20 and 40°c yielded the greatest pressure resistance in v. parahaemolyticus. the effects of salt concentration and h(2)o ...201121549045
characterization of toll-like receptor 3 gene in large yellow croaker, pseudosciaena crocea.toll-like receptor 3 (tlr3) plays an important role in innate immune responses. in this report, the full-length cdna sequence and genomic structure of pseudosciaena crocea tlr3 (pctlr3) were identified and characterized. the full-length cdna of pctlr3 was of 3384bp, including a 5'-terminal untranslated region (utr) of 65bp, a 3'-terminal utr of 589bp and an open reading frame (orf) of 2730bp encoding a polypeptide of 909 amino acid residues. the full-length genome sequence of pctlr3 was composed ...201121549197
transcriptional analysis of disk abalone (haliotis discus discus) antioxidant enzymes against marine bacteria and virus challenge.diverse antioxidant enzymes are essential for marine organisms to overcome oxidative stress as well as for the fine-tuning of immune reactions through activating different signal transduction pathways. this study describes the transcriptional analysis of antioxidant enzymes of disk abalone by challenging with bacteria (vibrio alginolyticus, vibrio parahemolyticus, and listeria monocytogenes) and viral hemorrhagic septicemia virus (vhsv). upon bacteria and vhsv challenge, manganese superoxide dis ...201121549200
genome sequence of a clinical o4:k12 serotype vibrio parahaemolyticus strain 10329.vibrio parahaemolyticus is the leading cause of foodborne illnesses worldwide. here we report a draft genome of v. parahaemolyticus strain vp10329 of o4:k12 serotype. it belongs to the main u. s. west coast clonal complex of v. parahaemolyticus (st36) causing oyster-associated human illness. it contains the virulence determinants tdh and trh but appears to infect at much lower doses than v. parahaemolyticus strains with these same determinants from other areas such as the u.s. gulf and atlantic ...201121551294
x-ray crystallography and isothermal titration calorimetry studies of the salmonella zinc transporter zntb.the zntb zn(2+) efflux system is important for maintenance of zn(2+) homeostasis in enterobacteria. we report crystal structures of zntb cytoplasmic domains from salmonella enterica serovar typhimurium (stzntb) in dimeric and physiologically relevant homopentameric forms at 2.3 å and 3.1 å resolutions, respectively. the funnel-like structure is similar to that of the homologous thermotoga maritima cora mg(2+) channel and a vibrio parahaemolyticus zntb (vpzntb) soluble domain structure. however, ...201121565704
aeromonas hydrophila moty is essential for polar flagellum function, requires coordinate expression of motx and pom proteins.by the analysis of aeromonas hydrophila atcc7966t genome we identified a. hydrophila ah-3 moty. a. hydrophila moty, like motx, is essential for the polar flagellum function energized by an electrochemical potential of na+ as coupling ion, but is not involved in lateral flagella function energized by proton motive force. thus, a. hydrophila polar flagellum stator is a complex integrated by two essential proteins: motx and moty, which interact with one of two redundant pairs of proteins: pomab and ...201121737499
occurrence and potential pathogenesis of vibrio cholerae, vibrio parahaemolyticus and vibrio vulnificus on the south coast of sweden.during the summer of 2006, several wound infections - of which three were fatal - caused by vibrio cholerae were reported from patients who had been exposed to water from the baltic sea. before these reports, we initiated a sampling project investigating the occurrence of potential human pathogenic v. cholerae, vibrio vulnificus and vibrio parahaemolyticus in the sound between sweden and denmark. the blue mussel (mytilus edulis) was used as an indicator to follow the occurrence of vibrios over t ...201121692819
quorum sensing and silencing in vibrio parahaemolyticus.the quorum regulatory cascade is poorly characterized in vibrio parahaemolyticus, in part because swarming and virulence factors-the hallmarks of the organism-are repressed by this scheme of gene control, and quorum sensing seems to be silenced in many isolates. in these studies, we examine a swarming-proficient, virulent strain and identify an altered-function allele of the quorum regulator luxo that is demonstrated to produce a constitutively active mimic of luxoôê+p. we find that luxo* affect ...201121705592
a novel genotyping scheme for vibrio parahaemolyticus with combined use of large variably-presented gene clusters (lvpcs) and variable-number tandem repeats (vntrs).a total of 18 variably-presented gene clusters (lvpcs) and nine previously characterized variable-number tandem repeats (vntrs), and all known virulence markers were screened for their frequency and/or copy number in 251 global strains of vibrio parahaemolyticus using pcr and gel or capillary electrophoresis. a two-step genotyping approach combining the use of lvpcs and vntrs was established accordingly. the frequency profiles of lvpcs and virulence markers were primarily used to group the strai ...201121742395
Conserved Tyrosine in the First Transmembrane Segment of Solute:Sodium Symporters Is Involved in Na+-coupled Substrate Co-transport.Solute:sodium symporters (SSSs) transport vital molecules across the plasma membrane of all living organisms. vSGLT, the Na(+)/galactose transporter of Vibrio parahemeolyticus, is the only SSS for which high resolution structural information is available, revealing a LeuT-like fold and a Na(+)-binding site analogous to the Na2 site of LeuT. Whereas the core transmembrane segments (TMs) of SSSs share high structural similarity with other transporters of LeuT-like fold, TM1 does not correspond to ...201121705334
Vibrio virulence genes in fishes collected from estuarine waters in Italy.Aims:ÔÇé The aim of this study was to investigate the presence of Vibrio vulnificus and potentially pathogenic strains of Vibrio parahaemolyticus in mullets collected from estuarine environment in Italy. Methods and Results:ÔÇé Two hundred and ninety-five mullets were analysed by culture using the selective medium thiosulfate citrate bile salt sucrose agar, during a monitoring period of 2ÔÇâyears (2008-2009). Presumptive Vibrio colonies were initially identified by using biochemical tests, and s ...201121740448
biological characterization of two marine bdellovibrio-and-like organisms isolated from daya bay of shenzhen, china and their application in the elimination of vibrio parahaemolyticus in oyster.bdellovibrio-and-like organisms (balos) are a group of highly motile delta-proteobacteria that prey on other gram-negative bacteria. however, nothing is known of the application potential of marine balos in safeguarding seafood safety. here, biological characterization of two marine balos strains and their application in the elimination of vibrio parahaemolyticus in oyster (crassostrea ariakensis) at the laboratory scale were investigated. balos strains bdh12 and bdhsh06 were isolated from sedim ...201121899909
occurrence of potentially pathogenic vibrios and related environmental factors in songkhla lake, thailand.vibrios are halophilic bacteria that are ubiquitous in marine environments. their occurrence in tropical lakes has rarely been investigated. in this study, the predominance and diversity of vibrio spp. was investigated over a 12-month period in a coastal lagoon, songkhla lake, in southern thailand. water samples were collected at 2 stations in the estuary near yor island in songkhla lake. the predominant vibrios were detected by a culture-based method, using thiosulfate-citrate-bile salt-sucrose ...201122014235
Molecular cloning and expression profiles of nitric oxide synthase (NOS) in mud crab Scylla paramamosain.The importance of the nitric oxide synthase (NOS) gene family is demonstrated by many studies in recent years. However, it keeps unknown of nitric oxide (NO) system and NOS gene family in mud crab Scylla paramamosain, an important cultured commercial crustacean in China and Pacific area. In this report, the cDNA of NOS contained full-length ORF was cloned from mud crab, S.paramamosain. It was of 4424 bp, including a 5'-terminal untranslated region (UTR) of 239 bp, a 3'-terminal UTR of 540 bp, wh ...201122200639
Detection and characterization of pathogenic vibrios in shellfish by a Ligation Detection Reaction-Universal Array approach.Vibrios are a group of major foodborne pathogens widely distributed in marine environment. Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus are the pathogenic species of Vibrio that pose the greatest threat to human health. However, other vibrios, e.g. Vibrio alginolyticus, Vibrio mimicus and Grimontia hollisae, apparently less relevant in the group of foodborne pathogens, have been sporadically found in outbreaks. For seafood safety and economic purposes, a rapid and powerful met ...201122177227
Bacteriophages with potential for inactivation of fish pathogenic bacteria: survival, host specificity and effect on bacterial community structure.Phage therapy may represent a viable alternative to antibiotics to inactivate fish pathogenic bacteria. Its use, however, requires the awareness of novel kinetics phenomena not applied to conventional drug treatments. The main objective of this work was to isolate bacteriophages with potential to inactivate fish pathogenic bacteria, without major effects on the structure of natural bacterial communities of aquaculture waters. The survival was determined in marine water, through quantification by ...201122163184
purification and characterization of enterocin mc13 produced by a potential aquaculture probiont enterococcus faecium mc13 isolated from the gut of mugil cephalus.a bacteriocin producer strain mc13 was isolated from the gut of mugil cephalus (grey mullet) and identified as enterococcus faecium. the bacteriocin of e. faecium mc13 was purified to homogeneity, as confirmed by tricine sodium dodecyl sulphate - polyacrylamide gel electrophoresis (sds-page). reverse-phase high-performance liquid chromatography (hplc) analysis showed a single active fraction eluted at 26 min, and matrix-assisted laser desorption ionization time of flight (maldi-tof) mass spectro ...201122112158
Oleic acid produced by a marine Vibrio spp. acts as an anti-Vibrio parahaemolyticus agent.It is known that some strains of Vibrio parahaemolyticus are responsible for gastroenteric diseases caused by the ingestion of marine organisms contaminated with these bacterial strains. Organic products that show inhibitory activity on the growth of the pathogenic V. parahaemolyticus were extracted from a Vibrio native in the north of Chile. The inhibitory organic products were isolated by reverse phase chromatography and permeation by Sephadex LH20, and were characterized by spectroscopic and ...201122073014
Prevalence and antimicrobial susceptibility of major foodborne pathogens in imported seafood.Seafood is a leading commodity implicated in foodborne disease outbreaks in the United States. Seafood importation rose dramatically in the past 3 decades and now contributes to more than 80% of the total U.S. seafood supply. However, limited data are available on the microbiological safety of imported seafood. In this study, we obtained a total of 171 salmon, shrimp, and tilapia samples imported from 12 countries in three retail stores in Baton Rouge, LA. The total microbial population and the ...201121902913
Mechanism of actin filament nucleation by Vibrio VopL and implications for tandem W domain nucleation.Pathogen proteins targeting the actin cytoskeleton often serve as model systems to understand their more complex eukaryotic analogs. We show that the strong actin filament nucleation activity of Vibrio parahaemolyticus VopL depends on its three W domains and on its dimerization through a unique VopL C-terminal domain (VCD). The VCD shows a previously unknown all-helical fold and interacts with the pointed end of the actin nucleus, contributing to the nucleation activity directly and through dupl ...201121873985
Characterization of anticancer, antimicrobial, antioxidant properties and chemical compositions of Peperomia pellucida leaf extract.Peperomia pellucida leaf extract was characterized for its anticancer, antimicrobial, antioxidant activities, and chemical compositions. Anticancer activity of P. pellucida leaf extract was determined through Colorimetric MTT (tetrazolium) assay against human breast adenocarcinoma (MCF-7) cell line and the antimicrobial property of the plant extract was revealed by using two-fold broth micro-dilution method against 10 bacterial isolates. Antioxidant activity of the plant extract was then charact ...201122071643
Sixty Years from the Discovery of Vibrio parahaemolyticus and Some Recollections.Vibrio parahaemolyticus was discovered by Tsunesaburo Fujino after a shirasu food poisoning outbreak in 1950, but at that time the isolate was named Pasteurella parahaemolytica, not Vibrio. Although the isolate resembled Vibrio, some properties did not correspond with those of Vibrio. For example, the curved cell form of the cell was one of the important taxonomical indicators of the genus, but the isolate was straight in form. After 5 years, Iwao Takikawa isolated a similar bacterium from a foo ...201122190435
a new twist in actin filament nucleation. 201121894217
High salinity relay as a postharvest processing strategy to reduce vibrio vulnificus levels in Chesapeake Bay oysters (Crassostrea virginica).In 2009 the U.S. Food and Drug Administration (FDA) announced its intention to implement postharvest processing (PHP) methods to eliminate Vibrio vulnificus from oysters intended for the raw, half-shell market that are harvested from the Gulf of Mexico during warmer months. FDA-approved PHP methods can be expensive and may be associated with unfavorable responses from some consumers. A relatively unexplored PHP method that uses relaying to high salinity waters could be an alternative strategy, ...201122054191
The structural pathway for water permeation through sodium-glucose cotransporters.Although water permeation across cell membranes occurs through several types of membrane proteins, the only permeation mechanism resolved at atomic scale is that through aquaporins. Crystallization of the Vibrio parahaemolyticus sodium-galactose transporter (vSGLT) allows investigation of putative water permeation pathways through both vSGLT and the homologous human Na-glucose cotransporter (hSGLT1) using computational methods. Grand canonical Monte Carlo and molecular dynamics simulations were ...201122004742
cloning and characterisation of the sptoll gene from green mud crab, scylla paramamosain.toll/toll-like receptors (tlrs), one of the most important pattern recognition receptors (prrs), play a crucial role in innate immune responses in both invertebrates and vertebrates. in this study, we cloned and characterised a toll gene from scylla paramamosain (sptoll). bioinformatic analysis predicted that sptoll contained one open reading frame of 3018bp and encoded a single-pass transmembrane domain protein of 1005 amino acids. further, sptoll could be clustered into one branch along with o ...201121945833
Identification of the Vibrio parahaemolyticus type III secretion system 2-associated chaperone VocC for the T3SS2-specific effector VopC.The enteropathogen Vibrio parahaemolyticus possesses two sets of type III secretion systems, T3SS1 and T3SS2. Effector proteins secreted by these T3SSs are delivered into host cells, leading to cell death or diarrhea. However, it is not known how specific effectors are secreted through a specific T3SS when both T3SSs are expressed within bacteria. One molecule thought to determine secretion specificity is a T3SS-associated chaperone; however, no T3SS2-specific chaperone has been identified. Ther ...201122092817
ecological determinants of the occurrence and dynamics of vibrio parahaemolyticus in offshore areas.the life cycle of vibrio parahaemolyticus has been conventionally associated with estuarine areas characterized by moderate salinity and warm seawater temperatures. recent evidence suggests that the distribution and population dynamics of v. parahaemolyticus may be shaped by the existence of an oceanic transport of communities of this organism mediated by zooplankton. to evaluate this possibility, the presence of v. parahaemolyticus in the water column of offshore areas of galicia was investigat ...201122094349
bis-(3'-5')-cyclic dimeric gmp-linked quorum sensing controls swarming in vibrio parahaemolyticus.movement over and colonization of surfaces are important survival strategies for bacteria, and many find it advantageous to perform these activities as a group, using quorum sensing to sample population size and synchronize behavior. it is puzzling however, that swarming-proficient and virulent strains of vibrio parahaemolyticus are silenced for the vibrio archetypal pathway of quorum sensing. here we describe the s-signal, a pheromone that can be communicated between cells in coculture to regul ...201122006340
the vibrio parahaemolyticus pvua1 gene (formerly termed psua) encodes a second ferric vibrioferrin receptor that requires tonb2.we previously reported that the vibrio parahaemolyticus pvsabcde and psua-pvuabcde operons are involved in the biosynthesis and transport of its own siderophore, vibrioferrin (vf). of these, psua and pvua encode tonb-dependent outer-membrane proteins (omps). although pvua was characterized as the ferric vibrioferrin receptor gene, the role of the psua product remains unknown. in this study, a growth assay of isogenic psua, pvua, and psua-pvua double-deletion mutants followed by complementation o ...201122092766
c-di-gmp-mediated decisions in the surface-grown vibrio parahaemolyticus: a different kind of motile-to-sessile transition.cyclic dimeric gmp (c-di-gmp) is a ubiquitous bacterial second messenger discovered over a quarter of century ago by moshe benziman and his colleagues at the hebrew university of jerusalem (15, 16).…201122194446
estimating the burden of acute gastroenteritis and foodborne illness caused by campylobacter, salmonella, and vibrio parahaemolyticus by using population-based telephone survey data, miyagi prefecture, japan, 2005 to 2006.most cases of acute gastroenteritis and foodborne disease are not ascertained by public health surveillance because the ill person does not always seek medical care and submit a stool sample for testing, and the laboratory does not always test for or identify the causative organism. we estimated the total burden of acute gastroenteritis in miyagi prefecture, japan, using data from two 2-week cross-sectional, population-based telephone surveys conducted in 2006 and 2007. to estimate the number o ...201122004803
structural analysis of the essential resuscitation promoting factor yeaz suggests a mechanism of nucleotide regulation through dimer reorganization.the yeaz gene product forms part of the conserved network yjee/yeaz/ygjd essential for the survival of many gram-negative eubacteria. among other as yet unidentified roles, yeaz functions as a resuscitation promoting factor required for survival and resuscitation of cells in a viable but non-culturable (vbnc) state.201121858042
[preparation and identification of ompw monoclonal antibodies].to prepare and characterize the mouse monoclonal antibodies against vibrio parahaemolyticus ompw.201121906472
development and evaluation of a loop-mediated isothermal amplification assay for rapid and sensitive detection of vibrio parahaemolyticus.loop-mediated isothermal amplification (lamp) assays targeting the rpod and toxr genes were developed to detect vibrio parahaemolyticus. all 78 tested v. parahaemolyticus strains yielded positive results within 40 min, while negative results were obtained for 69 strains of other organisms even at 60 min. for v. parahaemolyticus atcc 17802 in pure culture, the detection limits of lamp assays targeting rpod and toxr were 3.7 and 450 cfu per test, respectively. due to the higher sensitivity of rpod ...201121902914
[etiological surveillance and analysis of infectious diarrhea in beijing in year 2010].to explore the pathogenic form, epidemic features and serotype distribution of the pathogenic bacteria causing infectious diarrhea in beijing.201122177306
cloning and heterologous expression of the vibrioferrin biosynthetic gene cluster from a marine metagenomic library.a biosynthetic gene cluster of siderophore consisting of five open reading frames (orfs) was cloned by functional screening of a metagenomic library constructed from tidal-flat sediment. expression of the cloned biosynthetic genes in escherichia coli led to the production of vibrioferrin, a siderophore originally reported for the marine bacterium vibrio parahaemolyticus. to the best of our knowledge, this is the first example of heterologous production of a siderophore by biosynthetic genes clon ...201122146715
Characterization of Vibrio parahaemolyticus isolated from oysters and mussels in São Paulo, Brazil.Vibrio parahaemolyticus is a marine bacterium, responsible for gastroenteritis in humans. Most of the clinical isolates produce thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH) encoded by tdh and trh genes respectively. In this study, twenty-three V. parahaemolyticus, previously isolated from oysters and mussels were analyzed by PCR using specific primers for the 16S rRNA and virulence genes (tdh, trh and tlh) and for resistance to different classes of antibiotics and PFGE. Ni ...201121915463
cell death mediated by vibrio parahaemolyticus type iii secretion system 1 is dependent on erk1/2 mapk, but independent of caspases.vibrio parahaemolyticus, which causes gastroenteritis, wound infection, and septicemia, has two sets of type iii secretion systems (ttss), ttss1 and ttss2. a ttss1- deficient vcrd1 mutant of v. parahaemolyticus showed an attenuated cytotoxicity against hep-2 cells, and a significant reduction in mouse lethality, which were both restored by complementation with the intact vcrd1 gene. v. parahaemolyticus also triggered phosphorylation of mitogenactivated protein kinases (mapks) including p38 and e ...201121952366
environmental vibrio parahaemolyticus dna signatures validation.insignia is a novel dna computational system which uses highly efficient algorithms to compare bacterial genomes and to identify specific dna signatures to distinguish a target bacterium, or group of bacteria, from all other known bacterial species. it is currently being validated using different bacterial groups, including vibrio spp. in this study, the genomic analysis by insignia was conducted on vibrio parahaemolyticus, a halophilic gram-negative bacteria which constitutes a leading cause of ...201121940129
VopV, an F-actin-binding type III secretion effector, is required for Vibrio parahaemolyticus-induced enterotoxicity.Vibrio parahaemolyticus, a Gram-negative halophilic bacterium that causes acute gastroenteritis in humans, is characterized by two type III secretion systems (T3SS), namely T3SS1 and T3SS2. T3SS2 is indispensable for enterotoxicity but the effector(s) involved are unknown. Here, we identify VopV as a critical effector that is required to mediate V. parahaemolyticus T3SS2-dependent enterotoxicity. VopV was found to possess multiple F-actin-binding domains and the enterotoxicity caused by VopV cor ...201122018240
vibrio parahaemolyticus isolates from southeastern chinese coast are genetically diverse with circulation of clonal complex 3 strains since 2002.multilocus sequence typing (mlst) was used to examine the clonal relationship and genetic diversity of 71 vibrio parahaemolyticus isolates from clinical and seafood-related sources in southeastern chinese coast between 2002 and 2009. the tested isolates fell into 61 sequence types (sts). of 17 clinical isolates, 7 belonged to st3 of the pandemic clonal complex 3, with 3 strains isolated in 2002. although there was no apparent clonal relationship found between clinical strains and those from seaf ...201121883006
The unique role of siderophore in marine-derived Aureobasidium pullulans HN6.2.The L: -ornithine-N (5)-monooxygenase structural gene (SidA gene, accession number: FJ769160) was isolated from both the genomic DNA and cDNA of the marine yeast Aureobasidium pullulans HN6.2 by inverse PCR and RT-PCR. An open reading frame of 1,461 bp encoding a 486 amino acid protein (isoelectric point: 7.79) with calculated molecular weight of 55.4 kDa was characterized. The promoter of the gene (intronless) was located from -1 to -824 and had three HGATAR boxes which wer ...201121989767
Prevalence of Listeria monocytogenes, Vibrio parahaemolyticus, Staphylococcus aureus, and Salmonella spp. in Seafood Products Using Multiplex Polymerase Chain Reaction.Although several etiological agents can be transmitted through seafood consumption, Listeria monocytogenes, Vibrio parahaemolyticus, Staphylococcus aureus, and Salmonella spp. are considered among the most important pathogens in terms of public health and disease. In this study, multiplex polymerase chain reaction (PCR), as a rapid and cost-effective method, was used to determine the prevalence of these pathogens in 245 samples of raw/fresh, frozen, and ready-to-eat (RTE) seafood products market ...201122044288
Lactobacillus plantarum AS1 binds to cultured human intestinal cell line HT-29 and inhibits cell attachment by enterovirulent bacterium Vibrio parahaemolyticus.Lactobacillus plantarum AS1 was incubated with HT-29 adenocarcinoma cell line to assess its adhesion potency and examined for its inhibitory effect on the cell attachment by an enterovirulent bacterium Vibrio parahaemolyticus.201121848906
mechanism of actin filament nucleation by the bacterial effector vopl.vibrio parahaemolyticus protein l (vopl) is an actin nucleation factor that induces stress fibers when injected into eukaryotic host cells. vopl contains three n-terminal wiskott-aldrich homology 2 (wh2) motifs and a unique vopl c-terminal domain (vcd). we describe crystallographic and biochemical analyses of filament nucleation by vopl. the wh2 element of vopl does not nucleate on its own and requires the vcd for activity. the vcd forms a u-shaped dimer in the crystal, stabilized by a terminal ...201121873984
cdna cloning, characterization and expression analysis of a novel antimicrobial peptide gene penaeidin-3 (fi-pen3) from the haemocytes of indian white shrimp fenneropenaeus indicus.a new member of antimicrobial peptide genes of the penaeidin family, penaeidin 3, was cloned from the haemocytes of indian white shrimp fenneropeneaus indicus (f. indicus), by reverse transcription pcr (rt-pcr) and rapid amplification of cdna end (race-pcr) methods. the complete nucleotide sequence of cdna clone of indian white shrimp f. indicus penaeidin 3 (fi-pen3) was 243bp long and has an open reading frame which encodes 80 amino acid peptide. the homology analysis of fi-pen3 sequence with o ...201121885268
predictive models for the effect of storage temperature on vibrio parahaemolyticus viability and counts of total viable bacteria in pacific oysters (crassostrea gigas).vibrio parahaemolyticus is an indigenous bacterium of marine environments. it accumulates in oysters and may reach levels that cause human illness when postharvest temperatures are not properly controlled and oysters are consumed raw or undercooked. predictive models were produced by injecting pacific oysters (crassostrea gigas) with a cocktail of v. parahaemolyticus strains, measuring viability rates at storage temperatures from 3.6 to 30.4°c, and fitting the data to a model to obtain parameter ...201122003032
[analysis of main risk factors causing foodborne diseases in food catering business].to study main risk factors that cause foodborne diseases in food catering business.201121914339
Use of a DNA microarray for detection and identification of bacterial pathogens associated with fishery products.We established a microarray for the simultaneous detection and identification of diverse putative pathogens often associated with fishery products by targeting specific genes of Listeria monocytogenes, Salmonella, Shigella, Staphylococcus aureus, Streptococcus pyogenes, Vibrio cholerae, Vibrio parahaemolyticus, Vibrio vulnificus, and Yersinia enterocolitica and the 16S-23S rRNA gene internal transcribed spacer (ITS) region of Proteus mirabilis and Proteus vulgaris. The microarray contained 26 sp ...201121965411
Isolation and characterization of aerobic culturable arsenic-resistant bacteria from surfacewater and groundwater of Rautahat District, Nepal.Arsenic (As) contamination of groundwater is a serious Environmental Health Management issue of drinking water sources especially in Terai region of Nepal. Many studies have reported that due to natural abundance of arsenic in the environment, various bacteria have developed different resistance mechanisms for arsenic compound. In this study, the culturable arsenic-resistant bacteria indigenous to surfacewater as well as groundwater from Rautahat District of Nepal were randomly isolated by stand ...201121868146
vibrio illness in florida, 1998-2007.this study characterized the current epidemiology of vibrio infections in florida and examined cases reported from 1998 to 2007. logistic regression was used to determine risk of death. there were 834 vibrio infections in 825 individuals (average annual incidence rate 4·8/1,000,000). common vibrio species reported were vibrio vulnificus (33%), v. parahaemolyticus (29%), and v. alginolyticus (16%). most exposures were attributed to wounds (42%), and the most common clinical syndromes were wound i ...201120546636
Reactive oxygen system plays an important role in shrimp Litopenaeus vannamei defense against Vibrio parahaemolyticus and WSSV infection.The present study investigated the in vivo hemocytic and hepatopancreatic response to Vibrio parahaemolyticus and white spot syndrome virus (WSSV) injection in shrimp Litopenaeus vannamei. The proliferation of bacteria and virus in shrimp, animal mortality, total hemocyte counts (THCs), phenoloxidase (PO) activity, respiratory burst, and gene expression of immune factors associated with immune recognition (lectin), prophenoloxidase (proPO) activation, and the anti-microorganism (lysozyme) and ac ...201121991661
The Scr network in Vibrio parahaemolyticus: output targets and transcriptional regulation by a c-di-GMP responsive circuit.The Vibrio parahaemolyticus Scr system modulates decisions pertinent to surface colonization by affecting the cellular level of c-di-GMP. Here we explore the scope and mechanism of this regulation. Transcriptome comparison of ?scrABC and wild-type strains revealed expression differences with respect to ~100 genes. Elevated c-di-GMP repressed genes in the surface sensing regulon including those encoding the lateral flagellar and type three secretion systems and N-acetylglucosamine-binding protein ...201122194449
changes in caenorhabditis elegans exposed to vibrio parahaemolyticus.vibrio parahaemolyticus, which owes its origin to the marine environment, is considered as one of the most common causes of infectious diarrhea worldwide. the present study investigated the pathogenicity of v. parahaemolyticus against the model organism, caenorhabditis elegans. infection in the host was localized with gfp-tagged v. parahaemolyticus using confocal laser scanning microscopy. the times required for causing infection, bacterial load in intestine, chemotactic response, and alteration ...201122031026
functional characterization of the interaction between bacterial adhesin multivalent adhesion molecule 7 (mam7) protein and its host cell ligands.the ability of a pathogen to rapidly form a stable interaction with the host cell surface is key to its success. bacterial pathogens use a repertoire of virulence factors, but their efficient use relies on close contact between the host and the pathogen. we have recently identified a constitutively expressed mam7 (multivalent adhesion molecule 7), which is widely distributed in gram-negative pathogens and enables them to establish initial contact with the host cell. here, we describe the dissect ...201121937438
Vibrio jasicida sp. nov., a member of the Harveyi clade, from marine animals (packhorse lobster, abalone, and Atlantic salmon).Six isolates of a facultatively anaerobic bacterium were recovered in culture from marine invertebrates and vertebrates, including packhorse lobster (Jasus verreauxi), abalone (Haliotis sp.) and Atlantic salmon (Salmo salar) between 1994 and 2002. The bacteria are Gram-negative, rod shaped and motile by means of more than one polar flagellum, oxidase-positive, catalase-positive and able to grow between 0.5-8.0% NaCl (optimum 3.0-6.0%) and at temperatures of 10-37 °C (optimum 25-30 °C). On the ba ...201121984666
culture-independent real-time pcr reveals extensive polymicrobial infections in hospitalized diarrhoea cases in kolkata, india.clin microbiol infect abstract: culture-independent identification of diarrhoeal aetiological agents was performed using dna harvested from diarrhoeal stool specimens with sybr-green-based real-time pcr targeting vibrio cholerae, vibrio parahaemolyticus, campylobacter spp., shigella spp. and three different pathotypes of diarrhoeagenic escherichia coli. conventional culture-dependent methods detected bacterial enteropathogens in 68 of 122 diarrhoeal stool specimens. of 68 specimens, 59 (86.8%) h ...201122268636
retrieval of fractured resectoscope tips. 20113690197
longitudinal and cross-sectional assessments of age changes in physical strength as related to sex, social class, and mental ability. 20114833758
implications of drug levels in body fluids: basic concepts.drug concentrations in biological fluids are affected by the dose, route of administration, pattern of drug use, and the dispositional kinetics (distribution, metabolism, and excretion) of the drug. as most drugs are distributed to the site of action by blood, drug concentration measurement in this body fluid provides the best information as to the potential effect on behavior such as driving impairment or on psychological high. due to wide individual variations in the pharmacokinetics and pharm ...20113127724
deficiency of eosinophil peroxidase detected by automated cytochemistry. 20113122494
annual summary 1984. reported morbidity and mortality in the united states. 20113092006
tissue reaction to implant corrosion in 38 internal fixation devices.the corrosion characteristics, metallurgical properties, and clinical performance of 38 retrieved internal fixation devices were correlated with the tissue reaction to these devices. metallurgical parameters included thin and heavy inclusion content, rockwell hardness, and grain size. the excised fibrous tissue strip was directly overlying each plate at removal and sectioned between screw-hole sites. the material studied from the 38 plates consisted of 201 screw-hole junctions with associated ti ...20113368412
analysis of 585 burn patients hospitalized over a 6-year period. part ii: aetiological data.in a population of 585 burn patients from a well-defined urban area, the contribution of a number of factors to the occurrence of the burn injury is examined. these are: place where burned; activity of the patient at the time; contribution of various appliances and materials; the influence of clothing on the extent of the injury. these findings should prove helpful in future efforts at burn prevention.20113768750
pelvic fused kidneys: magnetic resonance imaging and intravenous pyelogram correlation.this is a case presentation of pelvic fused kidneys as demonstrated by intravenous pyelogram and magnetic resonance imaging (mri). the authors wish to stress the importance of anatomical imaging with mri in the coronal plane. no reconstruction is required as with computerized tomography.20113246706
[rheumatoid arthritis treated with penicillamine and bronchiolitis obliterans]. 20113231931
[epidemiology of blindness in the bouche-du-rhône area. methodological approach]. 20113168117
withdrawal of barbiturate anticonvulsant drugs: prospective controlled study.a barbiturate (phenobarbital or primidone) was withdrawn over a period of 3 months from 25 institutionalized residents, all of whom had had three seizures or less in the past 6 months and were maintained on a nonsedating drug (phenytoin, carbamazepine, or valproic acid). results were compared with a matched comparison group maintained on both drugs. subjects withdrawn from primidone, but not those withdrawn from phenobarbital, had increased seizure frequency, probably due to withdrawal. after 14 ...20113147687
possible interactions between zolpidem, a new sleep inducer and chlorpromazine, a phenothiazine neuroleptic.the combined use of a hypnotic and a neuroleptic is a rather frequent situation, encountered especially in the psychiatric sphere. we therefore tested zolpidem and chlorpromazine in six healthy subjects by using a double-blind latin square design. all of them received single doses of 20 mg zolpidem (zol), 50 mg chlorpromazine (cpz) and the combination of zol + cpz. the medication was given as a single dose in the morning and each treatment being separated by a 1-week interval. zolpidem produced ...20113147477
pseudoanaphylaxis: two case studies. 20113143943
lower oesophageal contractility as an indicator of brain death in paralysed and mechanically ventilated patients with head injury. 20113111608
[peyronie's disease: an objective review]. 20113070700
departmental divisions and the crisis in undergraduate medical education. 20113133048
the nature of time: implications for research on aging.contemporary research on aging gives little evidence of recognition of the underlying concepts of time. researchers have been much concerned with age as a variable but little with the explication of aging as a process. most authors do not define aging as they use the term or explain how time enters their causal explanations. in this article major concepts of time are reviewed and distinctions are made between physical, biological, psychological, and social time or age, which all might be classif ...20113076507
transfusion of bone marrow red cells during bone marrow harvests.large volumes of bone marrow may be required for certain types of autologous bone marrow transplants. the present study was done to determine whether red cells obtained during a bone marrow harvest would be useful in reducing homologous transfusion requirements. a group of patients receiving standard transfusion support during the harvest (group 1) was compared to a group that received processed bone marrow red cells (pbmrbc) (group 2). using the cobe 2991 cell processor, 90% of the harvested bo ...20113042443
[malignant lymphomas of various histological types in pike from the eastern portion of the baltic sea].malignant lymphomas have been found in pikes (esox lucius l.) caught in the eastern part of the baltic sea. the frequency of the disease has increased in recent years. three different histological types of malignant lymphoma are described in 32 pikes: poorly differentiated diffuse lymphocytic lymphoma, mixed (histiocytoid-lymphocytic) diffuse lymphoma and undifferentiated (anisomorphic) diffuse lymphoma.20113053150
[pharmacokinetics of non-steroidal anti-inflammatory agents in the elderly]. 20113067421
limitations in the use of 14c-glycocholate breath and stool bile acid determinations in patients with chronic diarrhea.analysis of a modified 14c-glycocholate breath test on 165 consecutive in-patients being investigated for chronic diarrhea showed that the measurement of 14co2 between 3 and 6 h after oral dosing of 5 microci of 14c-glycocholic acid was of only limited use to distinguish between patients with crohn's disease (cd), idiopathic bile salt wastage (ibw), or ileal resection (ir) from those with the irritable bowel syndrome (ibs). continuing 14co2 collections for up to 24 h was of little more help in e ...20113090133
psychochemistry in psychiatry. papers presented in memory of ole j. rafaelsen. 20113067535
[clinical pharmacology of converting enzyme inhibitors]. 20113375761
vascularization of male gonads in blennius pavo (teleostei, blenniidae) as revealed by scanning electron microscopy of vascular corrosion casts.the vascular architecture of male gonads of blennius pavo is studied by scanning electron microscopy of vascular corrosion casts. arterial supply to the gonads is by a branch of the first ventral segmental artery of the tail. from the surface of the gonads, this vessel gives rise to branches which supply testes, spermatic ducts, testicular glands, blind pouches, urogenital sinus and urogenital papilla. the testis has a rope-ladder-like capillary network around the seminiferous tubules, while in ...20113238381
[aids. less risk for the circumcised]. 20113209172
nonlinear accumulation of propranolol enantiomers.the accumulation of (+)- and (-)-propranolol was investigated in nine subjects who received 160 mg of racemic propranolol as a single dose and then once daily for 7 days. the serum concentrations of propranolol enantiomers were measured by h.p.l.c. using a novel chiral stationary phase allowing direct resolution of underivatized propranolol. the (+)-propranolol auc increased from 412 +/- 223 ng ml-1 h after single doses (0-infinity) to 584 +/- 279 ng ml-1 h at steady-state (0-24 h) (p less than ...20113203053
fetomaternal macrotransfusion in the yorkshire region. 2. perinatal outcome.the perinatal outcome associated with fetomaternal macrotransfusion (fmmt), defined as fetomaternal haemorrhage greater than or equal to 10 ml, was investigated in a study group of 179 cases of fmmt and compared with the outcome in 322 controls selected from the same population. perinatal mortality and morbidity occurred more frequently in the case group (p less than 0.01). the mean apgar score at 1 min and the cord haemoglobin level differed significantly between the case and control groups (p ...20113207644
poona district leprosy committee annual report for 1985-86. 20113192982
solving the rod-and-frame test in a tachistoscopic presentation: effects of stimulus size and perceptual style. 20113226894
metabolism of natural and synthetic steroids used in cancer treatment. 20113279431
metabolism of natural and synthetic steroids used in cancer treatment. 20113279431
the ohio medicaid drug formulary. 20113328840
the ohio medicaid drug formulary. 20113328840
Refresh
firstprev
...41424344454647484950...
nextlast
Displaying items 4101 - 4200 of 6009