Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| osmoadaptative strategy and its molecular signature in obligately halophilic heterotrophic protists. | halophilic microbes living in hypersaline environments must counteract the detrimental effects of low water activity and salt interference. some halophilic prokaryotes equilibrate their intracellular osmotic strength with the extracellular milieu by importing inorganic solutes, mainly potassium. these "salt-in" organisms characteristically have proteins that are highly enriched with acidic and hydrophilic residues. in contrast, "salt-out" halophiles accumulate large amounts of organic solutes li ... | 2016 | 27412608 |
| fungal peritonitis: underestimated disease in critically ill patients with liver cirrhosis and spontaneous peritonitis. | spontaneous peritonitis, especially spontaneous fungal peritonitis (sfp), is an important and potentially fatal complication in patients with endstage liver disaese. we evaluated potential risk factors, microbiological findings, and outcome of patients with sfp compared to spontaneous bacterial peritonitis (sbp) in critically ill patients. | 2016 | 27390856 |
| epidemiology and resistance patterns of bacterial and fungal colonization of biliary plastic stents: a prospective cohort study. | plastic stents used for the treatment of biliary obstruction will become occluded over time due to microbial colonization and formation of biofilms. treatment of stent-associated cholangitis is often not effective because of inappropriate use of antimicrobial agents or antimicrobial resistance. we aimed to assess the current bacterial and fungal etiology of stent-associated biofilms, with particular emphasis on antimicrobial resistance. | 2016 | 27171497 |
| key enzymes catalyzing glycerol to 1,3-propanediol. | biodiesel can replace petroleum diesel as it is produced from animal fats and vegetable oils, and it produces about 10 % (w/w) glycerol, which is a promising new industrial microbial carbon, as a major by-product. one of the most potential applications of glycerol is its biotransformation to high value chemicals such as 1,3-propanediol (1,3-pd), dihydroxyacetone (dha), succinic acid, etc., through microbial fermentation. glycerol dehydratase, 1,3-propanediol dehydrogenase (1,3-propanediol-oxydor ... | 2016 | 26966462 |
| the most common detected bacteria in sputum of patients with community acquired pneumonia (cap) treated in hospital. | community acquired pneumonia (cap) is the most common infective pulmonary disease. | 2016 | 27994296 |
| orthologous and paralogous ampd peptidoglycan amidases from gram-negative bacteria. | cell wall recycling and β-lactam antibiotic resistance are linked in enterobacteriaceae and in pseudomonas aeruginosa. this process involves a large number of murolytic enzymes, among them a cytoplasmic peptidoglycan amidase ampd, which plays an essential role by cleaving the peptide stem from key intermediates en route to the β-lactamase production (a resistance mechanism) and cell wall recycling. uniquely, p. aeruginosa has two additional paralogues of ampd, designated ampdh2 and ampdh3, which ... | 2016 | 27326855 |
| plasmid pec156, a naturally occurring escherichia coli genetic element that carries genes of the ecoviii restriction-modification system, is mobilizable among enterobacteria. | type ii restriction-modification systems are ubiquitous in prokaryotes. some of them are present in naturally occurring plasmids, which may facilitate the spread of these systems in bacterial populations by horizontal gene transfer. however, little is known about the routes of their dissemination. as a model to study this, we have chosen an escherichia coli natural plasmid pec156 that carries the ecoviii restriction modification system. the presence of this system as well as the cis-acting cer s ... | 2016 | 26848973 |
| functional properties of microorganisms in fermented foods. | fermented foods have unique functional properties imparting some health benefits to consumers due to presence of functional microorganisms, which possess probiotics properties, antimicrobial, antioxidant, peptide production, etc. health benefits of some global fermented foods are synthesis of nutrients, prevention of cardiovascular disease, prevention of cancer, gastrointestinal disorders, allergic reactions, diabetes, among others. the present paper is aimed to review the information on some fu ... | 2016 | 27199913 |
| focus on jnj-q2, a novel fluoroquinolone, for the management of community-acquired bacterial pneumonia and acute bacterial skin and skin structure infections. | jnj-q2 is a novel, fifth-generation fluoroquinolone that has excellent in vitro and in vivo activity against a variety of gram-positive and gram-negative organisms. in vitro studies indicate that jnj-q2 has potent activity against pathogens responsible for acute bacterial skin and skin structure infections (absssi) and community-acquired bacterial pneumonia (cabp), such as staphylococcus aureus and streptococcus pneumoniae. jnj-q2 also has been shown to have a higher barrier to resistance compar ... | 2016 | 27354817 |
| musculoskeletal infections associated with citrobacter koseri. | introduction citrobacter koseri is a well known cause of central nervous system infections in the paediatric setting. musculoskeletal infections caused by c koseri are rare, with only 14 previously reported cases. we present the first recorded case of c koseri induced septic arthritis of the knee along with a review of the literature. methods a search of the pubmed, embase(®) and google scholar™ databases was undertaken. only complete or near complete cases were reviewed. findings fourteen muscu ... | 2016 | 27412805 |
| a review of global initiatives to fight antibiotic resistance and recent antibiotics׳ discovery. | data from across the world have shown an overall decline in the antibiotic pipeline and continually rising resistance to all first-line and last-resort antibiotics. the gaps in our knowledge of existing prevalence and mechanisms of antibiotic resistance (abr) are all too well known. several decades of antibiotic abuse in humans, animals, and agricultural practices have created health emergency situations and huge socio-economic impact. this paper discusses key findings of the studies conducted b ... | 2016 | 27818921 |
| global dissemination of carbapenemase-producing klebsiella pneumoniae: epidemiology, genetic context, treatment options, and detection methods. | the emergence of carbapenem-resistant gram-negative pathogens poses a serious threat to public health worldwide. in particular, the increasing prevalence of carbapenem-resistant klebsiella pneumoniae is a major source of concern. k. pneumoniae carbapenemases (kpcs) and carbapenemases of the oxacillinase-48 (oxa-48) type have been reported worldwide. new delhi metallo-β-lactamase (ndm) carbapenemases were originally identified in sweden in 2008 and have spread worldwide rapidly. in this review, w ... | 2016 | 27379038 |
| antibiotic utilization patterns in patients with ventilator-associated pneumonia: a canadian context. | this retrospective cohort study describes the patterns of antibiotic use for the treatment of ventilator-associated pneumonia (vap) in the calgary zone of alberta health services. timing, appropriateness, and duration of antibiotics were evaluated in two hundred consecutive cases of vap derived from 4 adult intensive care units (icu). antibiotic therapy was initiated in less than 24 hours from vap diagnosis in 83% of cases. although most patients (89%) received empiric therapy that demonstrated ... | 2016 | 27525016 |
| cdi systems are stably maintained by a cell-contact mediated surveillance mechanism. | contact-dependent growth inhibition (cdi) systems are widespread amongst gram-negative bacteria where they play important roles in inter-cellular competition and biofilm formation. cdi+ bacteria use cell-surface cdia proteins to bind neighboring bacteria and deliver c-terminal toxin domains. cdi+ cells also express cdii immunity proteins that specifically neutralize toxins delivered from adjacent siblings. genomic analyses indicate that cdi loci are commonly found on plasmids and genomic islands ... | 2016 | 27355474 |
| consistent 1,3-propanediol production from glycerol in mixed culture fermentation over a wide range of ph. | glycerol is currently an over-produced chemical that can be used as substrate for the production of high value products such as 1,3-propanediol (1,3-pdo) in fermentation processes. the aim of this study was to investigate the effect of initial ph on a batch mixed culture fermentation of glycerol, considering both the bacterial community composition and the fermentation patterns. | 2016 | 26855671 |
| control of clostridium difficile physiopathology in response to cysteine availability. | the pathogenicity of clostridium difficile is linked to its ability to produce two toxins: tcda and tcdb. the level of toxin synthesis is influenced by environmental signals, such as phosphotransferase system (pts) sugars, biotin, and amino acids, especially cysteine. to understand the molecular mechanisms of cysteine-dependent repression of toxin production, we reconstructed the sulfur metabolism pathways of c. difficile strain 630 in silico and validated some of them by testing c. difficile gr ... | 2016 | 27297391 |
| noncanonical dna-binding mode of repressor and its disassembly by antirepressor. | dna-binding repressors are involved in transcriptional repression in many organisms. disabling a repressor is a crucial step in activating expression of desired genes. thus, several mechanisms have been identified for the removal of a stably bound repressor (rep) from the operator. here, we describe an uncharacterized mechanism of noncanonical dna binding and induction by a rep from the temperate salmonella phage spc32h; this mechanism was revealed using the crystal structures of homotetrameric ... | 2016 | 27099293 |
| nanosilver biocidal properties and their application in disinfection of hatchers in poultry processing plants. | the aim of this study was to use aqueous suspensions of silver nanoparticles with a wide spectrum of particle sizes, variable morphology, high stability, and appropriate physicochemical properties to examine their bactericidal and fungicidal properties against microorganisms present in poultry processing plants. at the same time, the particles were tested for preventing the production of odorogenous pollutants during incubation and thereby reducing the emission of harmful gases from such types o ... | 2016 | 26903785 |
| risk factors for bacteremia in patients with urinary catheter-associated bacteriuria. | catheter-associated bacteriuria is complicated by secondary bacteremia in 0.4% to 4.0% of cases. the directly attributable mortality rate is 12.7%. | 2016 | 27965229 |
| microbiome analysis reveals the abundance of bacterial pathogens in rousettus leschenaultii guano. | bats are crucial for proper functioning of an ecosystem. they provide various important services to ecosystem and environment. while, bats are well-known carrier of pathogenic viruses, their possible role as a potential carrier of pathogenic bacteria is under-explored. here, using culture-based approach, employing multiple bacteriological media, over thousand bacteria were cultivated and identified from rousettus leschenaultii (a frugivorous bat species), the majority of which were from the fami ... | 2016 | 27845426 |
| detection of ampc β-lactamase producing bacteria isolated in neonatal sepsis. | the objective of this study was to determine the occurrence and antimicrobial profile of ampc β-lactamase producing bacteria. | 2016 | 28083055 |
| genome sequences of nine gram-negative vaginal bacterial isolates. | the vagina is home to a wide variety of bacteria that have great potential to impact human health. here, we announce reference strains (now available through bei resources) and draft genome sequences for 9 gram-negative vaginal isolates from the taxa citrobacter, klebsiella, fusobacterium, proteus, and prevotella. | 2016 | 27688330 |
| correction: clinical impact of maldi-tof ms identification and rapid susceptibility testing on adequate antimicrobial treatment in sepsis with positive blood cultures. | [this corrects the article doi: 10.1371/journal.pone.0156299.]. | 2016 | 27658295 |
| carbapenem-resistant enterobacteriaceae: prevalence and risk factors in a single community-based hospital in korea. | carbapenemase-producing enterobacteriaceae (cpe) are gram-negative bacteria with increasing prevalence of infection worldwide. in korea, 25 cases of cpe isolates were reported by the korea centers for disease control and prevention in 2011. most cpe cases were detected mainly at tertiary referral hospitals. we analyzed the prevalence and risk factors for carbapenem-resistant enterobacteriaceae (cre) in a mid-sized community-based hospital in korea. | 2016 | 27659436 |
| a review of shv extended-spectrum β-lactamases: neglected yet ubiquitous. | β-lactamases are the primary cause of resistance to β-lactams among members of the family enterobacteriaceae. shv enzymes have emerged in enterobacteriaceae causing infections in health care in the last decades of the twentieth century, and they are now observed in isolates in different epidemiological settings both in human, animal and the environment. likely originated from a chromosomal penicillinase of klebsiella pneumoniae, shv β-lactamases currently encompass a large number of allelic vari ... | 2016 | 27656166 |
| assessment of the effectiveness of three different cephalosporins/clavulanate combinations for the phenotypic confirmation of extended spectrum beta lactamases producer bacterial isolates from urine samples at national public health laboratory, kathmandu, nepal. | the extended-spectrum β-lactamase (esbl) producing bacteria are present as the serious public health problems due to their resistance to large number of antibiotics. the main aims of this study were to determine the prevalence and antibiotic resistance patterns of bacteria producing extended-spectrum β-lactamases (esbls) and to find the suitable cephalosporin/clavulanate combination for phenotypic confirmation of esbl production. | 2016 | 27488224 |
| isolation and characterization of escherichia coli sequence type 131 and other antimicrobial-resistant gram-negative bacilli from clinical stool samples from veterans. | emerging multidrug-resistant (mdr) gram-negative bacilli (gnb), including escherichia coli sequence type 131 (st131) and its resistance-associated h30 subclone, constitute an ever-growing public health threat. their reservoirs and transmission pathways are incompletely defined. to assess diarrheal stools as a potential reservoir for st131-h30 and other mdr gnb, we cultured 100 clinical stool samples from a veterans affairs medical center clinical laboratory (october to december 2011) for fluoroq ... | 2016 | 27185805 |
| global dissemination of blakpc into bacterial species beyond klebsiella pneumoniae and in vitro susceptibility to ceftazidime-avibactam and aztreonam-avibactam. | the klebsiella pneumoniae carbapenemase (kpc), first described in the united states in 1996, is now a widespread global problem in several gram-negative species. a worldwide surveillance study collected gram-negative pathogens from 202 global sites in 40 countries during 2012 to 2014 and determined susceptibility to β-lactams and other class agents by broth microdilution testing. molecular mechanisms of β-lactam resistance among carbapenem-nonsusceptible enterobacteriaceae and pseudomonas aerugi ... | 2016 | 27161636 |
| comparative study of cronobacter identification according to phenotyping methods. | microbiological criteria applied to powdered infant formula (pif) require the absence of all cronobacter spp. consequently, misidentification of isolates from finished products can lead to significant financial losses for manufacturers and could increase the risk of neonatal infection. biochemical identification of suspect isolates using commercially available test panels is recommended for use by pif manufacturers by both the us fda and iso standard methods for cronobacter species; however, phe ... | 2016 | 27401027 |
| development of a phage cocktail to control proteus mirabilis catheter-associated urinary tract infections. | proteus mirabilis is an enterobacterium that causes catheter-associated urinary tract infections (cautis) due to its ability to colonize and form crystalline biofilms on the catheters surface. cautis are very difficult to treat, since biofilm structures are highly tolerant to antibiotics. phages have been used widely to control a diversity of bacterial species, however, a limited number of phages for p. mirabilis have been isolated and studied. here we report the isolation of two novel virulent ... | 2016 | 27446059 |
| antimicrobial activities of ceftazidime-avibactam and comparator agents against gram-negative organisms isolated from patients with urinary tract infections in u.s. medical centers, 2012 to 2014. | a total of 7,272 unique patient clinical isolates were collected from 71 u.s. medical centers from patients with urinary tract infections in 2012 to 2014 and tested for susceptibility to ceftazidime-avibactam and comparators by broth microdilution methods. ceftazidime-avibactam inhibited >99.9% of all enterobacteriaceae at the susceptible breakpoint of ≤8 μg/ml (there were only three nonsusceptible strains). ceftazidime-avibactam was also active against pseudomonas aeruginosa isolates (mic50, 2 ... | 2016 | 27114273 |
| clinical impact of maldi-tof ms identification and rapid susceptibility testing on adequate antimicrobial treatment in sepsis with positive blood cultures. | shortening the turn-around time (tat) of positive blood culture (bc) identification (id) and susceptibility results is essential to optimize antimicrobial treatment in patients with sepsis. we aimed to evaluate the impact on antimicrobial prescription of a modified workflow of positive bcs providing id and partial susceptibility results for enterobacteriaceae (eb), pseudomonas aeruginosa and staphylococcus aureus on the day of bc positivity detection. this study was divided into a pre-interventi ... | 2016 | 27228001 |
| vector potential of blattella germanica (l.) (dictyoptera: blattidae) for medically important bacteria at food handling establishments in jimma town, southwest ethiopia. | cockroaches have been regarded as possible vectors of human enteropathogens. their presence and crawl particularly in food handling establishments could be risky for human health. therefore, this study was done to determine the vector potential of cockroach for medically important bacterial pathogens in restaurants and cafeterias. a cross-sectional study was conducted on cockroaches from restaurants and cafeterias in jimma town from may to september 2014. standard taxonomic keys and microbiologi ... | 2016 | 27294115 |
| in vitro susceptibility to ceftazidime-avibactam of carbapenem-nonsusceptible enterobacteriaceae isolates collected during the inform global surveillance study (2012 to 2014). | the activity of ceftazidime-avibactam was assessed against 961 isolates of meropenem-nonsusceptible enterobacteriaceae most meropenem-nonsusceptible metallo-β-lactamase (mbl)-negative isolates (97.7%) were susceptible to ceftazidime-avibactam. isolates that carried kpc or oxa-48-like β-lactamases, both alone and in combination with extended-spectrum β-lactamases (esbls) and/or ampc β-lactamases, were 98.7% and 98.5% susceptible to ceftazidime-avibactam, respectively. meropenem-nonsusceptible, ca ... | 2016 | 26926648 |
| antimicrobial activities of ceftaroline and comparator agents against bacterial organisms causing bacteremia in patients with skin and skin structure infections in u.s. medical centers, 2008 to 2014. | we evaluated the antimicrobial susceptibility of 1,454 organisms consecutively collected from patients with bacteremia associated with skin and skin structure infections. the most common organisms obtained werestaphylococcus aureus(670 organisms [46.1%]),escherichia coli(200 organisms [13.8%]), β-hemolytic streptococci (βhs) (138 organisms [9.5%]), andklebsiella pneumoniae(109 organisms [7.5%]). the susceptibility rates for ceftaroline were 97.9% fors. aureus(95.9% among methicillin-resistants. ... | 2016 | 26856825 |
| insights into newer antimicrobial agents against gram-negative bacteria. | currently, drug resistance, especially against cephalosporins and carbapenems, among gram-negative bacteria is an important challenge, which is further enhanced by the limited availability of drugs against these bugs. there are certain antibiotics (colistin, fosfomycin, temocillin, and rifampicin) that have been revived from the past to tackle the menace of superbugs, including members of enterobacteriaceae, acinetobacter species, and pseudomonas species. very few newer antibiotics have been add ... | 2016 | 27013887 |
| evaluation of the septifast mgrade test on standard care wards--a cohort study. | the immediate need for appropriate antimicrobial therapy in septic patients requires the detection of the causative pathogen in a timely and reliable manner. in this study, the real-time pcr septifast mgrade test was evaluated in adult patients meeting the systemic inflammatory response syndrome (sirs) criteria that were treated at standard care wards. | 2016 | 26986826 |
| β-lactamase characterization of gram-negative pathogens recovered from patients enrolled in the phase 2 trials for ceftazidime-avibactam: clinical efficacies analyzed against subsets of molecularly characterized isolates. | the correlation of the clinical efficacies of ceftazidime-avibactam and comparators (carbapenems) was evaluated against baseline gram-negative isolates having characterized β-lactam resistance mechanisms from complicated urinary tract infection (cuti) and complicated intra-abdominal infection (ciai) phase 2 trials. enterobacteriaceae displaying ceftriaxone and/or ceftazidime mics of ≥ 2 μg/ml (69 isolates) and nonfermentative gram-negative bacilli (nf-gnb [three isolates]) with ceftazidime mics ... | 2016 | 26666936 |
| correlation of β-lactamase production and colistin resistance among enterobacteriaceae isolates from a global surveillance program. | the increasing use of carbapenems for treating multidrug-resistant (mdr) gram-negative bacterial infections has contributed to the global dissemination of carbapenem-resistant enterobacteriaceae (cre). serine and metallo-β-lactamases (mbls) that hydrolyze carbapenems have become prevalent and endemic in some countries, necessitating the use of older classes of agents, such as colistin. a total of 19,719 isolates of enterobacteriaceae (excluding proteeae and serratia spp., which have innate resis ... | 2016 | 26666920 |
| evaluation of an image analysis device (apas) for screening urine cultures. | while advancements have been made in some areas of pathology with diagnostic materials being screened using image analysis technologies, the reporting of cultures from agar plates remains a manual process. we compared the results for 2,163 urine cultures read by a reference panel of microbiologists, by the routine laboratory process, and by an automated plate reading system, apas (lbt innovations ltd., south australia). apas detected colonies with a sensitivity of 99.1% and a specificity of 99.3 ... | 2016 | 26582838 |
| detection of carbapenemase production in a collection of enterobacteriaceae with characterized resistance mechanisms from clinical and environmental origins by use of both carba np and blue-carba tests. | rapid-screening methods to confirm the presence of resistance mechanisms in multidrug-resistant bacteria are currently recommended. carba np and blue-carba tests were evaluated in carbapenemase-producing enterobacteriaceae from hospital (n = 102) and environmental (n = 57) origins for detecting the different molecular classes among them. both methods showed to be fast and cost-effective, with high sensitivity (98% to 100%) and specificity (100%), and may be easily introduced in the routine labor ... | 2016 | 26607979 |
| phenotypic detection and molecular characterization of beta-lactamase genes among citrobacter species in a tertiary care hospital. | to examine the distribution, emergence, and spread of genes encoding beta-lactamase resistance in citrobacter species isolated from hospitalized patients in a tertiary care hospital. | 2016 | 26952135 |
| draft genome sequence of providencia sneebia strain st1, a quorum sensing bacterium associated with marine microalgae. | providencia sneebia strain st1 is a symbiotic bacterium (belonging to phylum gammaproteobacteria) with marine microalgae. this bacterium exhibits the ability to produce n-acyl homoserine lactone signal molecule. to date, no genome that originates from marine providencia spp. has been reported. in this study, we present the genome sequence of this strain. it has a genome size of 4.89 m, with 19 contigs and an average g+c of 51.97%. the function of 4,631 proteins was predicted, and 3,652 proteins ... | 2016 | 27026792 |
| structural insights into the escherichia coli lysine decarboxylases and molecular determinants of interaction with the aaa+ atpase rava. | the inducible lysine decarboxylase ldci is an important enterobacterial acid stress response enzyme whereas ldcc is its close paralogue thought to play mainly a metabolic role. a unique macromolecular cage formed by two decamers of the escherichia coli ldci and five hexamers of the aaa+ atpase rava was shown to counteract acid stress under starvation. previously, we proposed a pseudoatomic model of the ldci-rava cage based on its cryo-electron microscopy map and crystal structures of an inactive ... | 2016 | 27080013 |
| employing bacterial microcompartment technology to engineer a shell-free enzyme-aggregate for enhanced 1,2-propanediol production in escherichia coli. | bacterial microcompartments (bmcs) enhance the breakdown of metabolites such as 1,2-propanediol (1,2-pd) to propionic acid. the encapsulation of proteins within the bmc is mediated by the presence of targeting sequences. in an attempt to redesign the pdu bmc into a 1,2-pd synthesising factory using glycerol as the starting material we added n-terminal targeting peptides to glycerol dehydrogenase, dihydroxyacetone kinase, methylglyoxal synthase and 1,2-propanediol oxidoreductase to allow their in ... | 2016 | 26969252 |
| comprehensive genome analysis of carbapenemase-producing enterobacter spp.: new insights into phylogeny, population structure, and resistance mechanisms. | knowledge regarding the genomic structure of enterobacter spp., the second most prevalent carbapenemase-producing enterobacteriaceae, remains limited. here we sequenced 97 clinical enterobacter species isolates that were both carbapenem susceptible and resistant from various geographic regions to decipher the molecular origins of carbapenem resistance and to understand the changing phylogeny of these emerging and drug-resistant pathogens. of the carbapenem-resistant isolates, 30 possessed blakpc ... | 2016 | 27965456 |
| microbiological quality of fresh nopal juice. | the consumption of fresh nopal cactus juice is widely popular among health-conscious consumers in mexico. the juice is prepared from fresh cladodes that have only been rinsed with tap water and are not subjected to a pasteurization or terminal bacterial reduction process. the aim of this study was to evaluate the microbial quality of commercially available fresh juices (n = 162) made with nopal in texcoco, state of mexico, during the summer and spring season. standard microbiological methods, th ... | 2016 | 27973398 |
| comparison of gram-negative and gram-positive hematogenous pyogenic spondylodiscitis: clinical characteristics and outcomes of treatment. | to the best of our knowledge, no study has compared gram-negative bacillary hematogenous pyogenic spondylodiscitis (gnb-hps) with gram-positive coccal hematogenous pyogenic spondylodiscitis (gpc-hps) regarding their clinical characteristics and outcomes. | 2016 | 27923346 |
| new hydrazines with sulphonamidic structure: synthesis,characterization and biological activity. | infections caused by bacterial species are common in immunocompromised patients and carry significant treatment costs and mortality. the emerging resistance of microorganisms to some synthetic antimicrobial agents makes it necessary to continue the research for new antimicrobial drugs. | 2016 | 24505922 |
| pathogenesis of mixed infection by spironucleus sp. and citrobacter freundii in freshwater angelfish pterophyllum scalare. | the present study was carried out to identify and describe the pathology of the freshwater angelfish pterophyllum scalare during chronic mortality in an in-door aquaculture system. scraping of the integument and gills and the collection of intestinal contents to search for external and internal parasites were performed. kidneys were collected aseptically for the microbiological analysis and the isolates were subjected to antibiotics to test for susceptibility. subsequently, necropsy for macrosco ... | 2016 | 27599811 |
| screening of nursing home residents for colonization with carbapenem-resistant enterobacteriaceae admitted to acute care hospitals: incidence and risk factors. | there are increasing reports of multidrug-resistant gram-negative bacilli in nursing homes and acute care hospitals. | 2016 | 26631643 |
| bacteremia due to citrobacter braakii: a case report and literature review. | among the citrobacter genus, the most commonly isolated bacteria from human specimens are citrobacter freundii and citrobacter koseri, and previous cases of infection due to citrobacter braakii have been rarely reported. we present a case of bacteremia due to c. braakii in a 38-year-old woman with cervical cancer. she was admitted to our hospital with complaints of a fever, chills, and nausea. blood culture results showed gram-negative bacilli identified as c. braakii via matrix-assisted laser d ... | 2016 | 27553069 |
| secondary metabolites extracted from marine sponge associated comamonas testosteroni and citrobacter freundii as potential antimicrobials against mdr pathogens and hypothetical leads for vp40 matrix protein of ebola virus: an in vitro and in silico investigation. | the current study explores therapeutic potential of metabolites extracted from marine sponge (cliona sp.)-associated bacteria against mdr pathogens and predicts the binding prospective of probable lead molecules against vp40 target of ebola virus. the metabolite-producing bacteria were characterized by agar overlay assay and as per the protocols in bergey's manual of determinative bacteriology. the antibacterial activities of extracted metabolites were tested against clinical pathogens by well-d ... | 2016 | 26577929 |
| lateral dissemination and inter-patient transmission of blakpc-3: role of a conjugative plasmid in spreading carbapenem resistance. | the objective of this study was to describe the nosocomial spread of carbapenemase-producing enterobacteria and characterize a plasmid involved in kpc dissemination. | 2016 | 26518052 |
| characterization of phicfp-1, a virulent bacteriophage specific for citrobacter freundii. | citrobacter freundii, a gram-negative bacterium, causes many opportunistic infections. bacteriophage phicfp-1 was isolated and characterized by its ability to lyse the multidrug-resistant clinical c. freundii strain p10159. transmission electron microscopy showed that the phage has an icosahedral head and a short tail, making it a podoviridae family member. in a single-step growth experiment, phicfp-1 exhibited an eclipse period of 20 min and a burst size of 100 particles per cell. its genome as ... | 2016 | 26455439 |
| analysis of the structure and function of fox-4 cephamycinase. | class c β-lactamases poorly hydrolyze cephamycins (e.g., cefoxitin, cefotetan, and moxalactam). in the past 2 decades, a new family of plasmid-based ampc β-lactamases conferring resistance to cefoxitin, the fox family, has grown to include nine unique members descended from the aeromonas caviae chromosomal ampc. to understand the basis for the unique cephamycinase activity in the fox family, we determined the first x-ray crystal structures of fox-4, apo enzyme and the acyl-enzyme with its namesa ... | 2016 | 26525784 |
| comparative study of a novel biochemical assay, the rapidec carba np test, for detecting carbapenemase-producing enterobacteriaceae. | the novel biochemical test, the rapidec carba np (rcnp), was evaluated using carbapenemase- and non-carbapenemase-producing enterobacteriaceae isolates. the rcnp test was compared with the carba np test (cnp) and the modified hodge test. compared to the cnp test, the rcnp test had identical sensitivity (96%) and lower specificity (93% versus 100%). the medium used to culture the isolates significantly affected test sensitivity and specificity. the rcnp test was quicker and easier to perform than ... | 2016 | 26582833 |
| high yield 1,3-propanediol production by rational engineering of the 3-hydroxypropionaldehyde bottleneck in citrobacter werkmanii. | imbalance in cofactors causing the accumulation of intermediates in biosynthesis pathways is a frequently occurring problem in metabolic engineering when optimizing a production pathway in a microorganism. in our previous study, a single knock-out citrobacter werkmanii ∆dhad was constructed for improved 1,3-propanediol (pdo) production. instead of an enhanced pdo concentration on this strain, the gene knock-out led to the accumulation of the toxic intermediate 3-hydroxypropionaldehyde (3-hpa). t ... | 2016 | 26822953 |
| a novel pcr-based approach for accurate identification of vibrio parahaemolyticus. | a pcr-based assay was developed for more accurate identification of vibrio parahaemolyticus through targeting the bla carb-17 like element, an intrinsic β-lactamase gene that may also be regarded as a novel species-specific genetic marker of this organism. homologous analysis showed that bla carb-17 like genes were more conservative than the tlh, toxr and atpa genes, the genetic markers commonly used as detection targets in identification of v. parahaemolyticus. our data showed that this bla car ... | 2016 | 26858713 |
| the salmonella in silico typing resource (sistr): an open web-accessible tool for rapidly typing and subtyping draft salmonella genome assemblies. | for nearly 100 years serotyping has been the gold standard for the identification of salmonella serovars. despite the increasing adoption of dna-based subtyping approaches, serotype information remains a cornerstone in food safety and public health activities aimed at reducing the burden of salmonellosis. at the same time, recent advances in whole-genome sequencing (wgs) promise to revolutionize our ability to perform advanced pathogen characterization in support of improved source attribution a ... | 2016 | 26800248 |
| diagnostic methods of helicobacter pylori infection for epidemiological studies: critical importance of indirect test validation. | among the methods developed to detect h. pylori infection, determining the gold standard remains debatable, especially for epidemiological studies. due to the decreasing sensitivity of direct diagnostic tests (histopathology and/or immunohistochemistry [ihc], rapid urease test [rut], and culture), several indirect tests, including antibody-based tests (serology and urine test), urea breath test (ubt), and stool antigen test (sat) have been developed to diagnose h. pylori infection. among the ind ... | 2016 | 26904678 |
| a novel 7-single nucleotide polymorphism-based clonotyping test allows rapid prediction of antimicrobial susceptibility of extraintestinal escherichia coli directly from urine specimens. | background. escherichia coli is a highly clonal pathogen. extraintestinal isolates belong to a limited number of genetically related groups, which often exhibit characteristic antimicrobial resistance profiles. methods. we developed a rapid clonotyping method for extraintestinal e coli based on detection of the presence or absence of 7 single nucleotide polymorphisms (snps) within 2 genes (fumc and fimh). a reference set of 2559 e coli isolates, primarily of urinary origin, was used to predict ... | 2016 | 26925427 |
| mechanism of the appabluf photocycle probed by site-specific incorporation of fluorotyrosine residues: effect of the y21 pka on the forward and reverse ground-state reactions. | the transcriptional antirepressor appa is a blue light using flavin (bluf) photoreceptor that releases the transcriptional repressor ppsr upon photoexcitation. light activation of appa involves changes in a hydrogen-bonding network that surrounds the flavin chromophore on the nanosecond time scale, while the dark state of appa is then recovered in a light-independent reaction with a dramatically longer half-life of 15 min. residue y21, a component of the hydrogen-bonding network, is known to be ... | 2016 | 26708408 |
| new monoclonal antibodies against a novel subtype of shiga toxin 1 produced by enterobacter cloacae and their use in analysis of human serum. | shiga toxin (stx) is a major virulence factor of several bacterial pathogens that cause potentially fatal illness, including escherichia coli and shigella spp. the continual emergence of new subtypes of stxs presents challenges for the clinical diagnosis of infections caused by stx-producing organisms. here, we report the development of four new monoclonal antibodies (mabs) against stx1e, a novel subtype of stx1 that was produced by an enterobacter cloacae strain and had limited reactivity with ... | 2016 | 27303707 |
| involvement of a novel class c beta-lactamase in the transglutaminase mediated cross-linking cascade of streptomyces mobaraensis dsm 40847. | streptomyces mobaraensis dsm 40847 secretes transglutaminase that cross-links proteins via γ-glutamyl-ε-lysine isopeptide bonds. characterized substrates are inhibitory proteins acting against various serine, cysteine and metalloproteases. in the present study, the bacterial secretome was examined to uncover additional transglutaminase substrates. fractional ethanol precipitation of the exported proteins at various times of culture growth, electrophoresis of the precipitated proteins, and sequen ... | 2016 | 26886195 |
| a highly precise and portable genome engineering method allows comparison of mutational effects across bacterial species. | currently available tools for multiplex bacterial genome engineering are optimized for a few laboratory model strains, demand extensive prior modification of the host strain, and lead to the accumulation of numerous off-target modifications. building on prior development of multiplex automated genome engineering (mage), our work addresses these problems in a single framework. using a dominant-negative mutant protein of the methyl-directed mismatch repair (mmr) system, we achieved a transient sup ... | 2016 | 26884157 |
| nonproliferative and proliferative lesions of the gastrointestinal tract, pancreas and salivary glands of the rat and mouse. | the inhand (international harmonization of nomenclature and diagnostic criteria for lesions in rats and mice) project is a joint initiative of the societies of toxicologic pathology from europe (estp), great britain (bstp), japan (jstp), and north america (stp) to develop an internationally accepted nomenclature and diagnostic criteria for nonproliferative and proliferative lesions in laboratory animals. the purpose of this publication is to provide a standardized nomenclature and diagnostic cri ... | 2016 | 26973378 |
| epidemiology and burden of bloodstream infections caused by extended-spectrum beta-lactamase producing enterobacteriaceae in a pediatric hospital in senegal. | severe bacterial infections are not considered as a leading cause of death in young children in sub-saharan africa. the worldwide emergence of extended-spectrum beta-lactamase producing enterobacteriaceae (esbl-e) could change the paradigm, especially in neonates who are at high risk of developing healthcare-associated infections. | 2016 | 26867226 |
| antimicrobial susceptibility and molecular epidemiology of extended-spectrum beta-lactamase-producing enterobacteriaceae from intensive care units at hamad medical corporation, qatar. | the emergence of extended-spectrum beta-lactamase (esbl)-producing isolates has important clinical and therapeutic implications. high prevalence of esbl-producing enterobacteriaceae has been reported in the literature for clinical samples from a variety of infection sites. the present study was undertaken to evaluate the prevalence of esbl-producing enterobacteriaceae, and to perform molecular characterization and antimicrobial susceptibility testing of clinical isolates from patients admitted t ... | 2016 | 26865975 |
| drug and vaccine development for the treatment and prevention of urinary tract infections. | urinary tract infections (uti) are among the most common bacterial infections in humans, affecting millions of people every year. uti cause significant morbidity in women throughout their lifespan, in infant boys, in older men, in individuals with underlying urinary tract abnormalities, and in those that require long-term urethral catheterization, such as patients with spinal cord injuries or incapacitated individuals living in nursing homes. serious sequelae include frequent recurrences, pyelon ... | 2016 | 26999391 |
| characterization of extended-spectrum beta-lactamase from escherichia coli and klebsiella species from north eastern nigeria. | resistance to antimicrobials has become a serious global health concern complicating treatment strategies and increasing health-care costs. the extended-spectrum beta-lactamase producing bacteria stand out as bacteria of great epidemic concern among gram negative bacilli. control and appropriate interventions for antimicrobial resistance depend on effective surveillance and knowledge of the patterns and determinants of resistance. | 2016 | 27042460 |
| characterization of a cellulomonas fimi exoglucanase/xylanase-endoglucanase gene fusion which improves microbial degradation of cellulosic biomass. | effective degradation of cellulose requires multiple classes of enzyme working together. however, naturally occurring cellulases with multiple catalytic domains seem to be rather rare in known cellulose-degrading organisms. a fusion protein made from cellulomonas fimi exo- and endo- glucanases, cex and cena which improves breakdown of cellulose is described. a homologous carbohydrate binding module (cbm-2) present in both glucanases was fused to give a fusion protein cxna. cxna or unfused constr ... | 2016 | 27702471 |
| characterization and genome sequencing of a citrobacter freundii phage cfp1 harboring a lysin active against multidrug-resistant isolates. | citrobacter spp., although frequently ignored, is emerging as an important nosocomial bacterium able to cause various superficial and systemic life-threatening infections. considered to be hard-to-treat bacterium due to its pattern of high antibiotic resistance, it is important to develop effective measures for early and efficient therapy. in this study, the first myovirus (vb_cfrm_cfp1) lytic for citrobacter freundii was microbiologically and genomically characterized. its morphology, activity ... | 2016 | 27683211 |
| ndm-producing enterobacteriaceae in a chinese hospital, 2014-2015: identification of ndm-producing citrobacterwerkmanii and acquisition of blandm-1-carrying plasmid in vivo in a clinical escherichia coli isolate. | new delhi metallo-β-lactamase (ndm)-producing enterobacteriaceae (npe) shows prevalence in china. little is known about the mechanisms related to the spread of npe. recently, a total of 51 non-duplicated npe isolates were collected from a tertiary-care hospital in china and analysed for genetic relatedness by pfge, antimicrobial susceptibility by etest and sequence type by multilocus sequence typing. s1-pfge and southern blot analysis or pcr amplification were used for plasmid profiling. between ... | 2016 | 27667823 |
| [transdiaphragmatic abscess by citrobacter freundii as a late complication of a laparoscopic cholecystectomy]. | 2016 | 27627452 | |
| antimicrobial resistance patterns of enterobacteriaceae in european wild bird species admitted in a wildlife rescue centre. | wild birds have been considered to be reservoirs of enteric human pathogens and vectors of resistance dissemination to the environment. during annual migration, they potentially play a role in the epidemiology of human associated zoonoses. the aim of this study was to investigate the frequency of isolation and antimicrobial susceptibility profiles of enterobacteriaceae members isolated from cloacal swabs of common european wild birds. fifty-five cloacal swabs were taken during birds' entrance ev ... | 2016 | 27393875 |
| multidrug-resistant enterobacteriaceae from indoor air of an urban wastewater treatment plant. | wastewater treatment plants (wwtps) have been recognized as sources of bioaerosols that may act as vehicles for dissemination of pathogens and multidrug-resistant (mdr) bacteria. the occurrence of mdr enterobacteriaceae in indoor air of an urban wwtp was investigated. a possible airborne contamination with extended-spectrum beta-lactamase (esbl) and carbapenemase-producing enterobacteriaceae was also explored. fourteen of 39 enterobacteriaceae isolates were mdr. these isolates were found at all ... | 2016 | 27260528 |
| molecular characterization of class b carbapenemases in advanced stage of dissemination and emergence of class d carbapenemases in enterobacteriaceae from croatia. | carbapenemases involved in acquired carbapenem resistance in enterobacteriaceae belong to ambler class a serin β-lactamases, class b metallo-β-lactamases (mbl) or class d oxa-48-like β-lactamases. the aim of the present study was to analyse the molecular epidemiology and the mechanisms and routes of spread of class b and class d carbapenemases in croatia. in total 68 isolates were analyzed. antibiotic susceptibility was determined by broth microdilution method. pcr was used to detect antibiotic- ... | 2016 | 27174090 |
| canaliculitis caused by citrobacter freundii. | 2016 | 27163908 | |
| mobilization of the salmonella genomic island sgi1 and the proteus genomic island pgi1 by the a/c2 plasmid carrying blatem-24 harboured by various clinical species of enterobacteriaceae. | the objective of this study was to transfer the salmonella genomic islands (gis) sgi1 and sgi1-v and the proteus gi pgi1-pmesc to clinical isolates of enterobacteriaceae harbouring an a/c2 plasmid. | 2016 | 27150396 |
| carbapenemase-producing enterobacteriaceae in hospital wastewater: a reservoir that may be unrelated to clinical isolates. | carbapenemase-producing enterobacteriaceae (cpe) are an emerging infection control problem in hospitals worldwide. identifying carriers may help reduce potential spread and infections. | 2016 | 27107615 |
| mechanisms of ertapenem resistance in enterobacteriaceae isolates in a tertiary university hospital. | the aim of this study was to investigate the molecular mechanisms of ertapenem resistance among enterobacteriaceae isolates in a clinical microbiology laboratory at a tertiary university hospital. a total of 40 clinical isolates including 20 resistant and 20 intermediate isolates were collected from august 2012 to july 2013. ertapenem susceptibility was confirmed by the broth microdilution method. pcr and sequencing analysis of carbapenemase, ampc β-lactamase, and extended-spectrum β-lactamase ( ... | 2016 | 27101841 |
| dissemination of the blakpc gene by clonal spread and horizontal gene transfer: comparative study of incidence and molecular mechanisms. | in addition to the global spread of the kpc-producing klebsiella pneumoniae (kpc-kp) clonal complex (cc)-258 clone, the blakpc gene may also spread by horizontal gene transfer (hgt), as suspected when more than one kpc-producing enterobacteriaceae (kpc-ent) species are isolated in a single patient. we aimed to characterize the incidence and molecular features of kpc-kp that were isolated alone (singular kpc-kp) versus kpc-kp that were isolated together with another kpc-ent species (joint kpc-kp) ... | 2016 | 27073266 |
| cesarean section changes neonatal gut colonization. | delivery by means of cesarean section has been associated with increased risk of childhood immune-mediated diseases, suggesting a role of early bacterial colonization patterns for immune maturation. | 2016 | 27045582 |
| cofactor-embedded nanoporous activated carbon matrices for the immobilization of intracellular enzymes and degradation of endocrine disruptor. | the mixed intracellular enzyme (mice) from citrobacter freundii, capable of degrading o-phenylene diamine (opd), was extracted and characterized. cofactors such as zinc and copper ions enhanced the mice activity. the functionalized nanoporous-activated carbon (fnac) matrix, zinc-impregnated fnac matrix (zn(2+) -fnac), copper-impregnated fnac matrix (cu(2+) -fnac), and zinc- and copper-impregnated fnac matrix (zn(2+) -cu(2+) -fnac) were prepared and characterized to immobilize mice. the parameter ... | 2016 | 26988244 |
| characteristics of extended-spectrum β-lactamase (esbl)- and pampc beta-lactamase-producing enterobacteriaceae of water samples in tunisia. | the presence of extended-spectrum beta-lactamase and plasmid-mediated ampc beta-lactamase producing enterobacteriaceae (esbl-eb and pampc-eb, respectively) was analyzed in 57 wastewater and 57 surface-water samples in tunisia. twenty-four of the 57 wastewater samples (42.1%) and one of the 57 surface-water samples (1.7%, a river that received effluents of a wastewater-treatment-plant) contained esbl-eb or pampc-eb; one esbl/pampc-eb per positive sample was further characterized. beta-lactamase g ... | 2016 | 26871556 |
| visual and efficient immunosensor technique for advancing biomedical applications of quantum dots on salmonella detection and isolation. | it is a great challenge in nanotechnology for fluorescent nanobioprobes to be applied to visually detect and directly isolate pathogens in situ. a novel and visual immunosensor technique for efficient detection and isolation of salmonella was established here by applying fluorescent nanobioprobes on a specially-designed cellulose-based swab (a solid-phase enrichment system). the selective and chromogenic medium used on this swab can achieve the ultrasensitive amplification of target bacteria and ... | 2016 | 26853517 |
| complex class 1 integron carrying qnrb62 and blavim-2 in a citrobacter freundii clinical isolate. | the coexistence of qnrb62 and blavim-2 was detected in a citrobacter clinical isolate. the reduced fluoroquinolone susceptibility is attributable to qnrb62, mutations of quinolone-resistance-determining regions, and an efflux pump or pumps. the genetic context surrounding chromosomal qnrb62 was a novel complex class 1 integron (in1184::iscr1::qnrb62) containing a unique gene array (blavim-2-aaca4'-8-gucd). an 18-nucleotide deletion at the 3' end of the pspa gene [pspa(δ18)], upstream of qnrb62, ... | 2016 | 27572415 |
| genome and plasmid analysis of blaimp-4-carrying citrobacter freundii b38. | sequencing of the blaimp-4-carrying c. freundii b38 using the pacbio smrt technique revealed that the genome contained a chromosome of 5,134,500 bp and three plasmids, poz172 (127,005 bp), poz181 (277,592 bp), and poz182 (18,467 bp). plasmid poz172 was identified as incfiiy, like pp10164-ndm and pndm-ecgn174. it carries a class 1 integron with four cassettes (blaimp-4-qacg2-aaca4-apha15) and a complete hybrid tni module (tnir-tniq-tnib-tnia). the recombination of tnir from tn402 (identical) with ... | 2016 | 27572407 |
| use of wgs data for investigation of a long-term ndm-1-producing citrobacter freundii outbreak and secondary in vivo spread of blandm-1 to escherichia coli, klebsiella pneumoniae and klebsiella oxytoca. | an outbreak of ndm-1-producing citrobacter freundii and possible secondary in vivo spread of blandm-1 to other enterobacteriaceae were investigated. | 2016 | 27494919 |
| characterization of extended-spectrum β-lactamase (esbl)-producing klebsiella, enterobacter, and citrobacter obtained in environmental samples of a tunisian hospital. | the assessment of the hospital environment as a reservoir of esbl-producing enterobacteriaceae in tunisian hospitals is scarcely analyzed, except for escherichia coli. the aim of this study was to evaluate the presence of esbl-producing non-e. coli enterobacteriaceae (esbl-ebnoec) in 300 samples of abiotic surfaces and the hands of patients and staff of a tunisian hospital, and to characterize the esbl genes of the recovered isolates. esbl-ebnoec were recovered in 28 of 300 (9.3%) analyzed sampl ... | 2016 | 27492133 |
| characteristics of ciprofloxacin-resistant enterobacteriaceae isolates recovered from wastewater of an algerian hospital. | hospital effluents are a source of environmental pollution by drugs, antibiotic-resistant bacteria, and resistance genes. quinolones, particularly ciprofloxacin, are commonly detected in these effluents, contributing to the emergence of antimicrobial resistance. the objective of this study was to characterize ciprofloxacin-resistant enterobacteriaceae in hospital effluents. | 2016 | 27482804 |
| prospective study using anterior approach did not show association between modic 1 changes and low grade infection in lumbar spine. | the modern literature is producing a rapidly growing number of articles which highlight the relationship between infection and lumbar disc degeneration. however, the means by which samples are collected is questionable. posterior approach surgery is not free from skin contamination. the possibility of intraoperative contamination of disc biopsies cannot be excluded. | 2016 | 26818032 |
| essential oils to control ichthyophthiriasis in pacu, piaractus mesopotamicus (holmberg): special emphasis on treatment with melaleuca alternifolia. | in vitro effect of the melaleuca alternifolia, lavandula angustifolia and mentha piperita essential oils (eos) against ichthyophthirius multifiliis and in vivo effect of m. alternifolia for treating ichthyophthiriasis in one of the most important south american fish, piaractus mesopotamicus (holmberg), were evaluated. the in vitro test consisted of three eos, each at concentrations of 57 μl l(-1) , 114 μl l (-1) , 227 μl l(-1) and 455 μl l (-1) , which were assessed once an hour for 4 h in micro ... | 2016 | 26776242 |
| synthesis and antibacterial characterization of sustainable nanosilver using naturally-derived macromolecules. | greener nanosynthesis utilizes fewer amounts of materials, water, and energy; while reducing or replacing the need for organic solvents. a novel approach is presented using naturally-derived flavonoids including quercetin pentaphosphate (qpp), quercetin sulfonic acid (qsa) and apigenin triphosphate (atrp). these water soluble, phosphorylated flavonoids were utilized both as reducing agent and stabilizer. the synthesis was achieved at room temperature using water as a solvent and it requires no c ... | 2016 | 26765510 |
| structure of methionine γ-lyase from clostridium sporogenes. | methionine γ-lyase (mgl) is a pyridoxal 5'-phosphate-dependent enzyme that catalyzes the γ-elimination reaction of l-methionine. the enzyme is a promising target for therapeutic intervention in some anaerobic pathogens and has attracted interest as a potential cancer treatment. the crystal structure of mgl from clostridium sporogenes has been determined at 2.37 å resolution. the fold of the protein is similar to those of homologous enzymes from citrobacter freundii, entamoeba histolytica, pseudo ... | 2016 | 26750487 |
| biodegradation of used engine oil by novel strains of ochrobactrum anthropi hm-1 and citrobacter freundii hm-2 isolated from oil-contaminated soil. | used engine oil (ueo) constitutes a serious environmental problem due to the difficulty of disposal off or reuse. ten bacterial strains with biodegradation potential were isolated from ueo-contaminated soil sample using enrichment technique. two strains which exhibited the highest degradation %, 51 ± 1.2 and 48 ± 1.5, respectively, were selected. based on the morphological, biochemical characteristics and 16s rrna sequence analysis, they were identified as ochrobactrum anthropi hm-1 (accession n ... | 2016 | 28330298 |
| study of the antibacterial and antifungal activities of synthetic benzyl bromides, ketones, and corresponding chalcone derivatives. | several applications of chalcones and their derivatives encouraged researchers to increase their synthesis as an alternative for the treatment of pathogenic bacterial and fungal infections. in the present study, chalcone derivatives were synthesized through cross aldol condensation reaction between 4-(n,n-dimethylamino)benzaldehyde and multiarm aromatic ketones. the multiarm aromatic ketones were synthesized through nucleophilic substitution reaction between 4-hydroxy acetophenone and benzyl bro ... | 2016 | 27877017 |
| enterohemorrhagic escherichia coli o157 in milk and dairy products from libya: isolation and molecular identification by partial sequencing of 16s rdna. | the aim of this work was to isolate and molecularly identify enterohemorrhagic escherichia coli (ehec) o157 in milk and dairy products in libya, in addition; to clear the accuracy of cultural and biochemical identification as compared with molecular identification by partial sequencing of 16s rdna for the existing isolates. | 2016 | 27956766 |
| finished genome sequence of the highly multidrug-resistant human urine isolate citrobacter freundii strain sl151. | citrobacter freundii is a gram-negative opportunistic pathogen that is increasingly being recognized as a causative agent of hospital-acquired urinary tract infections and an important reservoir of antimicrobial resistance determinants. in this report, we describe the finished genome sequence of c. freundii strain sl151, a highly multidrug-resistant human urine isolate. | 2016 | 27811104 |