Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| atazg1 and atazg2 comprise a novel family of purine transporters in arabidopsis. | in plants, nucleobase biochemistry is highly compartmented relying upon a well-regulated and selective membrane transport system. in arabidopsis two proteins, atazg1 and atazg2, show substantial amino acid sequence similarity to the adenine-guanine-hypoxanthine transporter azga of aspergillus nidulans. analysis of single and double mutant lines harboring t-dna insertion alleles atazg1-1 and atazg2-1 reveal a marked resistance to growth in the presence of 8-azaadenine and 8-azaguanine but not to ... | 2009 | 19121308 |
| response to hypoxia, reduction of electron acceptors, and subsequent survival by filamentous fungi. | filamentous fungi usually inhabit normoxic environments by utilizing oxygen as a substrate for respiration and for the biosynthesis of some essential cellular components. this review examines the metabolic mechanisms used by filamentous fungi under oxygen-limited (hypoxic) conditions. denitrification is one mechanism through which fusarium oxysporum and other fungi reduce nitrate or nitrite to nitrous oxide, generating nitric oxide as a reaction intermediate. the involvement of cytochrome p450no ... | 2009 | 19129650 |
| alternative processing of proproteins in aspergilli kexb gene disruptants under hyperosmotic conditions. | disruption of the kexb gene encoding a subtilisin-like processing protease in aspergillus oryzae and aspergillus nidulans led to remarkable morphological defects, and these phenotypes were suppressed under hyperosmotic conditions. in this study, we investigated to determine whether non-kexb proteases might complement the in vivo function of kexb in the two aspergillus kexb disruptants. neither overexpression of opsa or opsb encoding a. oryzae aspartyl proteases homologous to yeast yapsins (yps1/ ... | 2009 | 19129662 |
| inactivation of the lipoxygenase zmlox3 increases susceptibility of maize to aspergillus spp. | plant and fungal lipoxygenases (lox) catalyze the oxidation of polyunsaturated fatty acids, creating fatty-acid hydroperoxides (oxylipins). fungal oxylipins are required for normal fungal development and secondary metabolism, and plant host-derived oxylipins interfere with these processes in fungi, presumably by signal mimicry. the maize lox gene zmlox3 has been implicated previously in seed-aspergillus interactions, so we tested the interactions of a mutant maize line (lox3-4, in which zmlox3 i ... | 2009 | 19132874 |
| the 2008 update of the aspergillus nidulans genome annotation: a community effort. | the identification and annotation of protein-coding genes is one of the primary goals of whole-genome sequencing projects, and the accuracy of predicting the primary protein products of gene expression is vital to the interpretation of the available data and the design of downstream functional applications. nevertheless, the comprehensive annotation of eukaryotic genomes remains a considerable challenge. many genomes submitted to public databases, including those of major model organisms, contai ... | 2009 | 19146970 |
| indole prenyltransferases from fungi: a new enzyme group with high potential for the production of prenylated indole derivatives. | prenylated indole derivatives are hybrid natural products containing both aromatic and isoprenoid moieties and are widely spread in plants, fungi and bacteria. some of these complex natural products, e.g. the ergot alkaloids ergotamine and fumigaclavine c as well as the diketopiperazine derivative fumitremorgin c and its biosynthetic precursors tryprostatin a and b, show a wide range of biological and pharmacological activities. prenyl transfer reactions catalysed by prenyltransferases represent ... | 2009 | 19149573 |
| contribution of peroxisomes to penicillin biosynthesis in aspergillus nidulans. | peroxisomal localization of the third enzyme of the penicillin biosynthesis pathway of aspergillus nidulans, acyl-coenzyme a:ipn acyltransferase (iat), is mediated by its atypical peroxisomal targeting signal 1 (pts1). however, mislocalization of iat by deletion of either its pts1 or of genes encoding proteins involved in peroxisome formation or transport does not completely abolish penicillin biosynthesis. this is in contrast to the effects of iat mislocalization in penicillium chrysogenum. | 2009 | 19151327 |
| identification of virulence genes in fusarium oxysporum f. sp. lycopersici by large-scale transposon tagging. | forward genetic screens are efficient tools for the dissection of complex biological processes, such as fungal pathogenicity. a transposon tagging system was developed in the vascular wilt fungus fusarium oxysporum f. sp. lycopersici by inserting the novel modified impala element imp160::gfp upstream of the aspergillus nidulans niad gene, followed by transactivation with a constitutively expressed transposase. a collection of 2072 nia(+) revertants was obtained from reporter strain t12 and scree ... | 2009 | 19161356 |
| physiological involvement in ph signaling of vps24-mediated recruitment of aspergillus palb cysteine protease to escrt-iii. | activation of the aspergillus nidulans transcription factor pacc, which mediates ambient ph regulation of gene expression and is recruited to escrt-iii by the vps32-interacting scaffold pala, involves its ambient ph-dependent c-terminal proteolysis. this reaction is almost certainly catalyzed by the palb calpain-like protease. here we show that palb associates with membranes and interacts specifically and directly with escrt-iii vps24. the palb n-terminal mit domain and the vps24 c-terminal mim ... | 2009 | 19056728 |
| purine utilization by klebsiella oxytoca m5al: genes for ring-oxidizing and -opening enzymes. | the enterobacterium klebsiella oxytoca uses a variety of inorganic and organic nitrogen sources, including purines, nitrogen-rich compounds that are widespread in the biosphere. we have identified a 23-gene cluster that encodes the enzymes for utilizing purines as the sole nitrogen source. growth and complementation tests with insertion mutants, combined with sequence comparisons, reveal functions for the products of these genes. here, we report our characterization of 12 genes, one encoding gua ... | 2009 | 19060149 |
| overexpression of the chitosanase gene in fusarium solani via agrobacterium tumefaciens-mediated transformation. | to overexpress the chitosanase gene (csn) in f. solani, a vector based on pcambia 1300 was constructed. the csn gene, which is under control of the aspergillus nidulans gpda promoter and a. nidulans trpc terminator, was introduced back into the f. solani genome by agrobacterium tumefaciens-mediated transformation, and the herbicide-resistance gene bar from streptomyces hygroscopicus was used as the selection marker. transformants which showed a significant increase in chitosanase production (app ... | 2009 | 19093150 |
| occurrence and relevance of filamentous fungi in respiratory secretions of patients with cystic fibrosis--a review. | the colonization of airways by filamentous fungi and the development of respiratory infections require some predisposing factors as encountered in patients with cystic fibrosis (cf). indeed, the defective mucociliary clearance which characterizes the disease is associated with local immunological disorders. in addition, the prolonged therapy with antibiotics and the use of corticosteroid treatments also facilitate fungal growth. an important fungal biota has been described in respiratory secreti ... | 2009 | 19107638 |
| antifungal activity of anidulafungin, a product of aspergillus nidulans, against aspergillus nidulans. | anidulafungin is a semisynthetic product originating from aspergillus nidulans. the aim of this study was to determine whether this drug is active against a. nidulans strains. the minimum effective concentration (mec) of anidulafungin without and with 50% foetal calf serum was determined according to the clinical and laboratory standards microdilution method. all 13 a. nidulans strains were highly susceptible to anidulafungin, with a mec of 0.031mg/l. the presence of serum did not affect the in ... | 2009 | 19026523 |
| the aspergillus nidulans kinesin-3 unca motor moves vesicles along a subpopulation of microtubules. | the extremely polarized growth form of filamentous fungi imposes a huge challenge on the cellular transport machinery, because proteins and lipids required for hyphal extension need to be continuously transported to the growing tip. recently, it was shown that endocytosis is also important for hyphal growth. here, we found that the aspergillus nidulans kinesin-3 motor protein unca transports vesicles and is required for fast hyphal extension. most surprisingly, unca-dependent vesicle movement oc ... | 2009 | 19037104 |
| thematic issue: aspergillus genomics and beyond. preface. | 2009 | 19041727 | |
| aspergillus nidulans flbe is an upstream developmental activator of conidiation functionally associated with the putative transcription factor flbb. | aspergillus nidulans switches from vegetative growth to conidiation when aerial hyphae make contact with the atmosphere, or are subjected to specific environmental stress. the activation of the central conidiation pathway led by the transcription factor brla is a critical milestone in this morphogenetic transition. a number of upstream developmental activators (udas), expressed in vegetative cells, are required for this process to occur in conjunction with cessation of vegetative growth. mutants ... | 2009 | 19007409 |
| genetic regulation of aflatoxin biosynthesis: from gene to genome. | aflatoxins are notorious toxic secondary metabolites known for their impacts on human and animal health, and their effects on the marketability of key grain and nut crops. understanding aflatoxin biosynthesis is the focus of a large and diverse research community. concerted efforts by this community have led not only to a well-characterized biosynthetic pathway, but also to the discovery of novel regulatory mechanisms. common to secondary metabolism is the clustering of biosynthetic genes and th ... | 2009 | 19010433 |
| transcription regulation of the pbgp43 gene by nitrogen in the human pathogen paracoccidioides brasiliensis. | we show indirect evidences for the possible involvement of nit2-like binding motifs in transcription modulation of the pbgp43 gene, which codes for an important antigen from the human fungal pathogen paracoccidioides brasiliensis. this investigation was motivated by the finding of 23 nit2-like sites within the proximal -2047 nucleotides of the pbgp43 5' intergenic region from the pb339 isolate. they compose four clusters, two of them identical. we found four nit2-containing probes that were posi ... | 2009 | 19013535 |
| quality evaluation of co-composted wheat straw, poultry droppings and oil seed cakes. | poultry droppings, neem cake, castor cake, jatropha cake and grass clippings were used separately as organic nitrogen additives to decrease the high c:n ratio of wheat straw. composting was carried out aerobically in presence of fungal consortium developed by including aspergillus awamori, aspergillus nidulans, trichoderma viride and phanerochaete chrysosporium. the degraded product was characterized to assess the technical viability of organic nitrogen supplements as well as fungal consortium i ... | 2009 | 19015937 |
| the three fungal transmembrane nuclear pore complex proteins of aspergillus nidulans are dispensable in the presence of an intact an-nup84-120 complex. | in aspergillus nidulans nuclear pore complexes (npcs) undergo partial mitotic disassembly such that 12 npc proteins (nups) form a core structure anchored across the nuclear envelope (ne). to investigate how the npc core is maintained, we affinity purified the major core an-nup84-120 complex and identified two new fungal nups, an-nup37 and an-elys, previously thought to be vertebrate specific. during mitosis the an-nup84-120 complex locates to the ne and spindle pole bodies but, unlike vertebrate ... | 2009 | 19019988 |
| a novel highly acidic beta-mannanase from the acidophilic fungus bispora sp. mey-1: gene cloning and overexpression in pichia pastoris. | using degenerate polymerase chain reaction (pcr) and thermal asymmetric interlaced pcr, a 1,347-bp full-length complementary dna fragment encompassing the gene man5a, which encodes a 429-amino acid beta-mannanase with a calculated mass of 46.8 kda, was cloned from acidophilic bispora sp. mey-1. the deduced amino acid sequence (catalytic domain) displayed highest identity (54.1%) with the emericella nidulans endo-beta-1,4-d-mannanase, a member of the glycoside hydrolase family 5. recombinant man5 ... | 2009 | 18998121 |
| long-distance movement of aspergillus nidulans early endosomes on microtubule tracks. | in fungal hyphal cells, intracellular membrane trafficking is constrained by the relatively long intracellular distances and the mode of growth, exclusively by apical extension. endocytosis plays a key role in hyphal tip growth, which involves the coupling of secretory membrane delivery to the apical region with subapical compensatory endocytosis. however, the identity, dynamics and function of filamentous fungal endosomal compartments remain largely unexplored. aspergillus nidulans raba(rab5) l ... | 2009 | 19000168 |
| effect of bioaugmentation and nitrogen supplementation on composting of paddy straw. | a composting experiment was conducted to evaluate the effect of a hyperlignocellulolytic fungal consortium and different nitrogen amendments on paddy straw composting in terms of changes in physicochemical and biological parameters. a fungal consortium comprising four lignocellulolytic mesophilic fungal cultures was used as inoculum for bioaugmentation of paddy straw in perforated pits. the comparative effect of farmyard manure (fym), soybean trash, poultry litter and urea on the composting proc ... | 2009 | 18839317 |
| overexpression of a novel endogenous nadh kinase in aspergillus nidulans enhances growth. | the complete genome sequence of the filamentous fungi aspergillus nidulans has paved the way for fundamental research on this industrially important species. to the best of our knowledge, this is the first time a gene encoding for atp-dependent nadh kinase (atp:nadh 2'-phosphotransferase, ec 2.7.1.86) has been identified. the enzyme has a predicted molecular weight of 49 kda. we characterised the role of this nadh kinase by genomic integration of the putative gene an8837.2 under a strong constit ... | 2009 | 18840540 |
| analysis and prediction of gene splice sites in four aspergillus genomes. | several aspergillus fungal genomic sequences have been published, with many more in progress. obviously, it is essential to have high-quality, consistently annotated sets of proteins from each of the genomes, in order to make meaningful comparisons. we have developed a dedicated, publicly available, splice site prediction program called netaspgene, for the genus aspergillus. gene sequences from aspergillus fumigatus, the most common mould pathogen, were used to build and test our model. compared ... | 2009 | 18948220 |
| the level of sulfane sulfur in the fungus aspergillus nidulans wild type and mutant strains. | the interdependence of the sulfane sulfur metabolism and sulfur amino acid metabolism was studied in the fungus aspergillus nidulans wild type strain and in mutants impaired in genes encoding enzymes involved in the synthesis of cysteine (a precursor of sulfane sulfur) or in regulatory genes of the sulfur metabolite repression system. it was found that a low concentration of cellular cysteine leads to elevation of two sulfane sulfurtransferases, rhodanase and cystathionine gamma-lyase, while the ... | 2009 | 18781374 |
| annotation of stress-response proteins in the aspergilli. | stress-response proteins of aspergillus nidulans, aspergillus clavatus, aspergillus flavus, aspergillus fumigatus, aspergillus niger, aspergillus oryzae, aspergillus terreus and neosartorya fischeri (3908 in total) were annotated and grouped according to stress types (http://193.6.155.82/aspergillusstress/). all genomes harboured elements of the sska-hoga/saka stress signalling pathway. there are accumulating data pointing at the importance of sska-hoga/saka signalling in different types of stre ... | 2009 | 18703157 |
| functional diversity of chitin synthases of aspergillus nidulans in hyphal growth, conidiophore development and septum formation. | fungal chitin synthases are classified into seven classes. recent advances in genome sequence analyses have revealed that there are more than seven chitin synthase-encoding genes in the genome of filamentous ascomycete fungi. to clarify the functional differences among these genes in the morphogenesis and hyphal tip growth, we have cloned six chitin synthase-encoding genes from aspergillus nidulans and analyzed their functions. the results obtained suggest that chitin synthases are structurally ... | 2009 | 18651309 |
| prophylaxis against aspergillus is not perfect: problems and perils in stem cell transplantation. | availability of newer, well tolerated anti-aspergillus drugs at a time of rising prevalence of invasive aspergillosis has sparked enthusiasm for chemoprophylaxis against aspergillus in allogeneic stem cell recipients. such a drug-oriented approach however is associated with problems. in the azole class, itraconazole has been shown to be effective, but hepatotoxicity and gastrointestinal intolerability have made the drug less attractive. posaconazole, approved for prophylaxis in allogeneic stem c ... | 2009 | 18663660 |
| purification and partial characterization of the xanthine-uric acid transporter (uapa) of aspergillus nidulans. | uapa, the uric acid-xanthine permease from the filamentous ascomycete aspergillus nidulans, is one of the most thoroughly characterized purine/h(+) transporters in eukaryotes. detailed studies have addressed its regulation of expression, at both the transcriptional and post-translational levels, in response to physiological and developmental signals. an extensive kinetic profile towards a plethora of purines and mutational analyses have established models on how uapa recognizes the purine ring a ... | 2009 | 18824106 |
| the cypome (cytochrome p450 complement) of aspergillus nidulans. | the cytochromes p450 (cyps) are found in all biological kingdoms and genome sequencing projects continue to reveal an ever increasing number. the principle aim of this paper is to identify the complete cypome of aspergillus nidulans from the genome sequence version an.3 deposited at the broad institute, assign the appropriate cyp nomenclature and define function where possible. the completed analysis revealed a total of 111 cyp genes, 3 of which were previously unknown and 8 pseudogenes, represe ... | 2009 | 18824241 |
| genotoxicity of achillea millefolium essential oil in diploid cells of aspergillus nidulans. | the essential oil of achillea millefolium is commonly used in folk medicine for the treatment of several diseases and has been demonstrated previously to exert an in vitro antimicrobial activity against human pathogens. current study investigates the genotoxic activity of a. millefolium oil. the oil's major constituents are: chamazulene (42.15%), sabinene (19.72%), terpin-4-ol (5.22%), beta-caryophyllene (4.44%) and eucalyptol (3.10%), comprising 74.63% of the total. the oil's genotoxic evaluati ... | 2009 | 18803228 |
| a survey of nonribosomal peptide synthetase (nrps) genes in aspergillus nidulans. | the genome of aspergillus nidulans carries 27 genes encoding nonribosomal peptide synthetase (nrps) structures, although only five of these forming peptides and amino acid containing metabolites have been identified so far. this manuscript describes domain structures, substrate binding pockets and related genes and gene clusters and summarizes our current state of product prediction of fungal nrps systems. | 2009 | 18804170 |
| mapping the interaction sites of aspergillus nidulans phytochrome fpha with the global regulator vea and the white collar protein lreb. | aspergillus nidulans senses red and blue-light and employs a phytochrome and a neurospora crassa white collar (wc) homologous system for light perception and transmits this information into developmental decisions. under light conditions it undergoes asexual development and in the dark it develops sexually. the phytochrome fpha consists of a light sensory domain and a signal output domain, consisting of a histidine kinase and a response regulator domain. previously it was shown that the phytochr ... | 2009 | 18936976 |
| a single channel for nitrate uptake, nitrite export and nitrite uptake by escherichia coli naru and a role for nirc in nitrite export and uptake. | two related polytopic membrane proteins of the major facilitator family, nark and naru, catalyse nitrate uptake, nitrite export and nitrite uptake across the escherichia coli cytoplasmic membrane by an unknown mechanism. a 12-helix model of naru was constructed based upon six alkaline phosphatase and beta-galactosidase fusions to nark and the predicted hydropathy for the nark family. fifteen residues conserved in the nark-naru protein family were substituted by site-directed mutagenesis, includi ... | 2009 | 18691156 |
| proteins involved in microbody biogenesis and degradation in aspergillus nidulans. | fungal microbodies (peroxisomes) are inducible organelles that proliferate in response to nutritional cues. proteins involved in peroxisome biogenesis/proliferation are designated peroxins and are encoded by pex genes. an autophagy-related process, termed pexophagy, is responsible for the selective removal of peroxisomes from the cell. several genes involved in pexophagy are also required for autophagy and are collectively known as atg genes. we have re-analysed the aspergillus nidulans genome f ... | 2009 | 18694841 |
| recombinant bacterial hemoglobin alters metabolism of aspergillus niger. | the filamentous fungus aspergillus niger is used extensively for the production of enzymes and organic acids. a major problem in industrial fermentations with this fungus is to ensure sufficient supply of oxygen required for respiratory metabolism of the fungus. in case of oxygen limitation, the fungus will produce various by-products like organic acids and polyols. in order to circumvent this problem we here study the effects of the expression of a bacterial hemoglobin protein on the metabolism ... | 2009 | 18694843 |
| molecular detection and identification of aspergillus spp. from clinical samples using real-time pcr. | the definite and rapid diagnosis of invasive aspergillosis is necessary because of the high mortality caused. the objective of this study was to evaluate a real-time pcr assay to detect aspergillus spp. in clinical samples, based on the light cycler technology. specificity was assessed by using dna extracted from pathogenic and non-pathogenic bacteria/fungi from spanish collection including: two aspergillus flavus, four aspergillus fumigatus, two aspergillus nidulans, two aspergillus niger and t ... | 2009 | 18643921 |
| effect of culture temperature on the heterologous expression of pleurotus eryngii versatile peroxidase in aspergillus hosts. | production of recombinant versatile peroxidase in aspergillus hosts was optimized through the modification of temperature during bioreactor cultivations. to further this purpose, the cdna encoding a versatile peroxidase of pleurotus eryngii was expressed under control of the alcohol dehydrogenase (alca) promoter of aspergillus nidulans. a dependence of recombinant peroxidase production on cultivation temperature was found. lowering the culture temperature from 28 to 19 degrees c enhanced the lev ... | 2009 | 18481101 |
| characterisation of the cipc-like protein afua_5g09330 of the opportunistic human pathogenic mould aspergillus fumigatus. | aspergillus fumigatus is currently the major airborne fungal pathogen that menaces immunocompromised individuals. germination of inhaled conidia is a hallmark of the early infection process, but little is known about the underlying mechanisms. the intention of our ongoing studies is the identification of a. fumigatus proteins that are differentially expressed during germination and may provide insights in the germination process. using a proteomic approach, we identified afua_5g09330 as a major ... | 2010 | 19486301 |
| recombinantly produced hydrophobins from fungal analogues as highly surface-active performance proteins. | hydrophobins are available from natural resources only in milligram amounts. basf succeeded in a recombinant production process, up-scaled to pilot plant production in kilogram scale. strain and protein optimization by modulation of gene expression and generation of fusion proteins finally leads to two class i hydrophobins called h*protein a and h*protein b. by analytical ultracentrifugation, we confirm that the self-association of h*proteins in solution is governed by their sequence, because ol ... | 2010 | 19290518 |
| the roles played by aspergillus nidulans apoptosis-inducing factor (aif)-like mitochondrial oxidoreductase (aifa) and nadh-ubiquinone oxidoreductases (ndea-b and ndia) in farnesol resistance. | farnesol (foh) is a nonsterol isoprenoid produced by dephosphorylation of farnesyl pyrophosphate, a catabolite of the cholesterol biosynthetic pathway. these isoprenoids inhibit proliferation and induce apoptosis. here, we show that aspergillus nidulans aifa encoding the apoptosis-inducing factor (aif)-like mitochondrial oxidoreductase plays a role in the function of the mitochondrial complex i. additionally, we demonstrated that ndea-b and ndia encode external and internal alternative nadh dehy ... | 2010 | 20654725 |
| aspernidine a and b, prenylated isoindolinone alkaloids from the model fungus aspergillus nidulans. | 2010 | 20661238 | |
| a cryptic role of a glycolytic-gluconeogenic enzyme (aldolase) in amino acid transporter turnover in aspergillus nidulans. | in aspergillus nidulans the fbaa1013 mutation results in reduced or total loss of growth on glycolytic and gluconeogenic carbon sources, respectively. it also negatively affects growth on several amino acids (including l-proline, l-glutamate or l-aspartate) that the fungus can use as nitrogen source on glycolytic carbon sources. complementation of the fbaa1013 mutation using an a. nidulans genomic library resulted in cloning of the fbaa gene, which encodes a putative fructose 1,6-biphosphate ald ... | 2010 | 20026236 |
| localization and function of calmodulin in live-cells of aspergillus nidulans. | calmodulin (cam) is a small, eukaryotic protein that reversibly binds ca(2+). study of cam localization in genetically tractable organisms has yielded many insights into cam function. here, we described the dynamic localization of aspergillus nidulans cam (ancam) in live-cells by using recombination strains with homologous, single cross-over insertions at the target gene which placed the gfp fused copy under the inducible alca promoter and the rfp-cam integration under the native cam promoter. w ... | 2010 | 20034586 |
| septins aspa and aspc are important for normal development and limit the emergence of new growth foci in the multicellular fungus aspergillus nidulans. | septins are cytoskeletal proteins found in fungi, animals, and microsporidia, where they form multiseptin complexes that act as scaffolds recruiting and organizing other proteins to ensure normal cell division and development. here we characterize the septins aspa and aspc in the multicellular, filamentous fungus aspergillus nidulans. mutants with deletions of aspa, aspc, or both aspa and aspc show early and increased germ tube and branch emergence, abnormal septation, and disorganized conidioph ... | 2010 | 19949047 |
| the l279p mutation of nuclear distribution gene c (nudc) influences its chaperone activity and lissencephaly protein 1 (lis1) stability. | lis1, a gene mutated in classical lissencephaly, plays essential roles in cytoplasmic dynein regulation, mitosis and cell migration. however, the regulation of lis1 (lissencephaly protein 1) protein remains largely unknown. genetic studies in aspergillus nidulans have uncovered that the nud (nuclear distribution) pathway is involved in the regulation of cytoplasmic dynein complex and a temperature-sensitive mutation in the nudc gene (l146p) greatly reduces the protein levels of nudf, an aspergil ... | 2010 | 20675372 |
| a comparative study of activity and apparent inhibition of fungal β-glucosidases. | β-glucosidases (bgs) from aspergillus fumigates, aspergillus niger, aspergillus oryzae, chaetomium globosum, emericella nidulans, magnaporthe grisea, neurospora crassa, and penicillium brasilianum were purified to homogeneity, and analyzed by isothermal titration calorimetry with respect to their hydrolytic activity and its sensitivity to glucose (product) using cellobiose as substrate. global non-linear regression of several reactions, with or without added glucose, to a product inhibition equa ... | 2010 | 20677177 |
| gamma-tubulin regulates the anaphase-promoting complex/cyclosome during interphase. | a cold-sensitive gamma-tubulin allele of aspergillus nidulans, mipad159, causes defects in mitotic and cell cycle regulation at restrictive temperatures that are apparently independent of microtubule nucleation defects. time-lapse microscopy of fluorescently tagged mitotic regulatory proteins reveals that cyclin b, cyclin-dependent kinase 1, and the ancdc14 phosphatase fail to accumulate in a subset of nuclei at restrictive temperatures. these nuclei are permanently removed from the cell cycle, ... | 2010 | 20679430 |
| aspergillus nidulans alpha-galactosidase of glycoside hydrolase family 36 catalyses the formation of alpha-galacto-oligosaccharides by transglycosylation. | the alpha-galactosidase from aspergillus nidulans (aglc) belongs to a phylogenetic cluster containing eukaryotic alpha-galactosidases and alpha-galacto-oligosaccharide synthases of glycoside hydrolase family 36 (gh36). the recombinant aglc, produced in high yield (0.65 g.l(-1) culture) as his-tag fusion in escherichia coli, catalysed efficient transglycosylation with alpha-(1-->6) regioselectivity from 40 mm 4-nitrophenol alpha-d-galactopyranoside, melibiose or raffinose, resulting in a 37-74% y ... | 2010 | 20681989 |
| evolutionary genetics: desperate times call for more sex. | a new study has found that strains of the fungus aspergillus nidulans produce more of their spores sexually in environments where they are less fit, resembling a hypothesized transitional stage in the evolution of sex. | 2010 | 20692611 |
| eisosome organization in the filamentous ascomycete aspergillus nidulans. | eisosomes are subcortical organelles implicated in endocytosis and have hitherto been described only in saccharomyces cerevisiae. they comprise two homologue proteins, pil1 and lsp1, which colocalize with the transmembrane protein sur7. these proteins are universally conserved in the ascomycetes. we identify in aspergillus nidulans (and in all members of the subphylum pezizomycotina) two homologues of pil1/lsp1, pila and pilb, originating from a duplication independent from that extant in the su ... | 2010 | 20693301 |
| endocytic machinery protein slab is dispensable for polarity establishment but necessary for polarity maintenance in hyphal tip cells of aspergillus nidulans. | the aspergillus nidulans endocytic internalization protein slab is essential, in agreement with the key role in apical extension attributed to endocytosis. we constructed, by gene replacement, a nitrate-inducible, ammonium-repressible slab1 allele for conditional slab expression. video microscopy showed that repressed slab1 cells are able to establish but unable to maintain a stable polarity axis, arresting growth with budding-yeast-like morphology shortly after initially normal germ tube emerge ... | 2010 | 20693304 |
| inadvertent gene silencing of argininosuccinate synthase (bcass1) in botrytis cinerea by the plob1 vector system. | for several years, researchers working on the plant pathogen botrytis cinerea and a number of other related fungi have routinely used the plob1 vector system, based on hygromycin resistance, under the control of the aspergillus nidulans olic promoter and what was reported to be the beta-tubulin (tuba) terminator. recently, it has been demonstrated that this vector contains a 446-bp portion of the b. cinerea argininosuccinate synthase gene (bcass1) rather than the tuba terminator. as argininosucc ... | 2010 | 20696000 |
| the mode of reproduction in natural populations of ascomycetous fungus, emericella nidulans, from israel. | the mode of reproduction of the soil ascomycetous fungus emericella nidulans of israeli populations was studied using 15 microsatellite (simple sequence repeats or ssr) trinucleotide markers. the study was performed in three canyons: two located in the northern part of israel (mount carmel and western upper galilee) and one in the southern negev desert. in each canyon, e. nidulans strains were isolated from the opposite slopes and (in the desert canyon) the valley bottom. testing the reproductiv ... | 2010 | 20515512 |
| role of phospholipase c and protein kinase c in aspergillus nidulans during growth on pectin or glucose: effects on germination and duplication cycle. | the effects of plc and pkc inhibitors on aspergillus nidulans depend on the carbon source. plc inhibitors spm and c48/80 delayed the first nuclear division in cultures growing on glucose, but stimulated it in media supplemented with pectin. less intense were these effects on the mutant transformed with plc-a gene rupture (ap27). neomycin also delayed the germination in cultures growing on glucose or pectin; however, on glucose, the nuclear division was inhibited whereas in pectin it was stimulat ... | 2010 | 20526834 |
| aspergillus rabb rab5 integrates acquisition of degradative identity with the long distance movement of early endosomes. | aspergillus nidulans early endosomes display characteristic long-distance bidirectional motility. simultaneous dual-channel acquisition showed that the two rab5 paralogues rabb and raba colocalize in these early endosomes and also in larger, immotile mature endosomes. however, rabb-gtp is the sole recruiter to endosomes of vps34 pi3k (phosphatidylinositol-3-kinase) and the phosphatidylinositol-3-phosphate [pi(3)p] effector anvps19 and rabb delta, leading to thermosensitivity prevents multivesicu ... | 2010 | 20534811 |
| antifungal activity of eugenol against penicillium, aspergillus, and fusarium species. | the antifungal activity of eugenol in a model system against aspergilli (aspergillus niger, aspergillus terreus, and emericella nidulans), penicilli (penicillium expansum, penicillium glabrum, and penicillium italicum), and fusaria (fusarium oxysporum and fusarium avenaceum) was investigated. minimum detection time (time to attain a colony diameter of 1 cm) and the kinetic parameters were evaluated. the effectiveness of the active compound seemed to be strain or genus dependent; 100 mg/liter rep ... | 2010 | 20537272 |
| methylenetetrahydrofolate reductase activity is involved in the plasma membrane redox system required for pigment biosynthesis in filamentous fungi. | methylenetetrahydrofolate reductases (mthfrs) play a key role in biosynthesis of methionine and s-adenosyl-l-methionine (sam) via the recharging methionine biosynthetic pathway. analysis of 32 complete fungal genomes showed that fungi were unique among eukaryotes by having two mthfrs, met12 and met13. the met12 type contained an additional conserved sequence motif compared to the sequences of met13 and mthfrs from other eukaryotes and bacteria. targeted gene replacement of either of the two mthf ... | 2010 | 20543064 |
| self-protection against gliotoxin--a component of the gliotoxin biosynthetic cluster, glit, completely protects aspergillus fumigatus against exogenous gliotoxin. | gliotoxin, and other related molecules, are encoded by multi-gene clusters and biosynthesized by fungi using non-ribosomal biosynthetic mechanisms. almost universally described in terms of its toxicity towards mammalian cells, gliotoxin has come to be considered as a component of the virulence arsenal of aspergillus fumigatus. here we show that deletion of a single gene, glit, in the gliotoxin biosynthetic cluster of two a. fumigatus strains, rendered the organism highly sensitive to exogenous g ... | 2010 | 20548963 |
| the effect of resource quantity and resource stoichiometry on microbial carbon-use-efficiency. | the carbon-use-efficiency (cue) of microorganisms is an important parameter in determining ecosystem-level carbon (c) cycling; however, little is known about how variance in resources affects microbial cue. to elucidate how resource quantity and resource stoichiometry affect microbial cue, we cultured four microorganisms - two fungi (aspergillus nidulans and trichoderma harzianum) and two bacteria (pectobacterium carotovorum and verrucomicrobium spinosum) - under 12 unique c, nitrogen (n) and ph ... | 2010 | 20550579 |
| recombinant production of an aspergillus nidulans class i hydrophobin (dewa) in hypocrea jecorina (trichoderma reesei) is promoter-dependent. | fungal hydrophobins have potential for several applications because of their abilities to change the hydrophobicity of different surfaces. yet because of their tendency for aggregation and attachment to interfacial areas only few production processes have so far been reported. towards the development of a heterologous production system, we report here the expression of a class i hydrophobin dewa of aspergillus nidulans in hypocrea jecorina (trichoderma reesei). using the h. jecorina hfb2 (class ... | 2010 | 20567818 |
| spotlight on aspergillus nidulans photosensory systems. | aspergilli are ubiquitous soil-borne fungi growing within or on the surface of numerous organic substrates. growth within a substrate or growth on the surface correlates to different growth conditions for the hyphae due to significant changes in oxygen or reactive oxygen species levels and variations in humidity or temperature. the production of air-borne spores is supported by the substrate-air interphase and also requires a sensing system to adapt appropriately. here we focus on light as impor ... | 2010 | 20573560 |
| thermotolerance of germlings and mycelium of the insect-pathogenic fungus metarhizium spp. and mycelial recovery after heat stress. | the upper temperature limits for germination and growth were determined for metarhizium acridum (arsef 324, 3341 and 3609), m. robertsii (arsef 2575), m. anisopliae (arsef 5749), and aspergillus nidulans (atcc 10074). most of the metarhizium species germinated well at 35 and 36 degrees c; however, compared to 28 degrees c, the growth was very slow (except arsef 5749 from mexico, which germinated at 35 degrees c, but did not grow at 34 or 36 degrees c). germination was severely impaired at 38 and ... | 2010 | 20586069 |
| wild-type mic distributions and epidemiological cutoff values for the triazoles and six aspergillus spp. for the clsi broth microdilution method (m38-a2 document). | clinical breakpoints have not been established for mold testing. wild-type (wt) mic distributions (organisms in a species/drug combination with no detectable acquired resistance mechanisms) were defined in order to establish epidemiologic cutoff values (ecvs) for five aspergillus spp. and itraconazole, posaconazole, and voriconazole. also, we have expanded prior ecv data for aspergillus fumigatus. the number of available isolates varied according to the species/triazole combination as follows: 1 ... | 2010 | 20592159 |
| role of nek6 in tumor promoter-induced transformation in jb6 c141 mouse skin epidermal cells. | nek6 (nima-related kinase 6) is a homologue of the aspergillus nidulans protein nima (never in mitosis, gene a). we demonstrate that overexpression of nek6 induces anchorage-independent transformation of jb6 cl41 mouse epidermal cells. tissue arrays and western immunoblot analysis show that nek6 is overexpressed in malignant tissues and several cancer cell lines. our data also show that nek6 interacts with stat3, an oncogenic transcription factor, and phosphorylates stat3 on ser(727), which is i ... | 2010 | 20595392 |
| fitness-associated sexual reproduction in a filamentous fungus. | sex is a long-standing evolutionary enigma. although the majority of eukaryotes reproduce sexually at least sometimes [1-3], the evolution of sex from an asexual ancestor has been difficult to explain because it requires sexually reproducing lineages to overcome the manifold costs of sex, including the destruction of favorable gene combinations created by selection [4, 5]. conditions for the evolution of sex are much broader if individuals can reproduce either sexually or asexually (i.e., facult ... | 2010 | 20598542 |
| aspergillus nidulans striatin (stra) mediates sexual development and localizes to the endoplasmic reticulum. | striatin family proteins have been identified in animals and fungi and are considered to be scaffolding proteins. in fungi striatin orthologs have been associated with sexual development and virulence to plants. in this study, we characterized the functions and localization of the striatin ortholog, stra, in aspergillus nidulans. deltastra strains showed multiple defects in conidium germination, mycelial radial growth, production of diffusible red pigment, and reduced conidiation. the most strik ... | 2010 | 20601045 |
| the extracellular β-1,3-endoglucanase enga is involved in autolysis of aspergillus nidulans. | to elucidate the roles of the β-1,3-endoglucanase enga in autolysis of the filamentous fungus aspergillus nidulans and to identify the common regulatory elements of autolytic hydrolases. | 2010 | 20602653 |
| flbc is a putative nuclear c2h2 transcription factor regulating development in aspergillus nidulans. | asexual development (conidiation) in aspergillus is governed by multiple regulators. here, we characterize the upstream developmental activator flbc in aspergillus nidulans. flbc mrna is detectable throughout the life cycle, at relatively high levels during vegetative growth, early asexual and late sexual developmental phases. the deletion of flbc causes a delay/reduction in conidiation, brla and vosa expression, and conidial germination. while overexpression of flbc (oeflbc) does not elaborate ... | 2010 | 20624219 |
| urea, the major urea/h+ symporter in aspergillus nidulans. | we report here the characterization of urea, a high-affinity urea/h+ symporter of aspergillus nidulans. the deletion of the encoding gene abolishes urea transport at low substrate concentrations, suggesting that in these conditions urea is the sole transport system specific for urea in a. nidulans. the urea gene is not inducible by urea or its precursors, but responds to nitrogen metabolite repression, necessitating for its expression the area gata factor. in contrast to what was observed for ot ... | 2010 | 20633690 |
| bioinspired deposition of tio2 thin films induced by hydrophobins. | the deposition of ceramic thin films from aqueous solutions at low temperature using biopolymers as templates has attracted much attention due to economic and environmental benefits. titanium dioxide is one of the most attractive functional materials and shows a wide range of applications across vastly different areas because of its unique chemical, optical, and electrical properties. in the present work, we deposited smooth, nanocrystalline titania thin films by an aqueous deposition method on ... | 2010 | 20121159 |
| atfa bzip-type transcription factor regulates oxidative and osmotic stress responses in aspergillus nidulans. | the aim of the study was to demonstrate that the bzip-type transcription factor atfa regulates different types of stress responses in aspergillus nidulans similarly to atf1, the orthologous 'all-purpose' transcription factor of schizosaccharomyces pombe. heterologous expression of atfa in a s. pombe deltaatf1 mutant restored the osmotic stress tolerance of fission yeast in surface cultures to the same level as recorded in complementation studies with the atf1 gene, and a partial complementation ... | 2010 | 20131067 |
| heterochromatic marks are associated with the repression of secondary metabolism clusters in aspergillus nidulans. | fungal secondary metabolites are important bioactive compounds but the conditions leading to expression of most of the putative secondary metabolism (sm) genes predicted by fungal genomics are unknown. here we describe a novel mechanism involved in sm-gene regulation based on the finding that, in aspergillus nidulans, mutants lacking components involved in heterochromatin formation show de-repression of genes involved in biosynthesis of sterigmatocystin (st), penicillin and terrequinone a. durin ... | 2010 | 20132440 |
| the concerted action of bzip and cmyb transcription factors flbb and flbd induces brla expression and asexual development in aspergillus nidulans. | fungi are capable of generating diverse cell types through developmental processes that stem from hyphae, acting as pluripotent cells. the formation of mitospores on emergence of hyphae to the air involves the participation of transcription factors, which co-ordinate the genesis of new cell types, eventually leading to spore formation. in this investigation, we show that bzip transcription factor flbb, which has been attributed to participate in transducing the aerial stimulus signal, activates ... | 2010 | 20132447 |
| characterization of the aspergillus nidulans monodictyphenone gene cluster. | deletion of ccla, a component of the compass complex of aspergillus nidulans, results in the production of monodictyphenone and emodin derivatives. through a set of targeted deletions in a ccla deletion strain, we have identified the genes required for monodictyphenone and emodin analog biosynthesis. identification of an intermediate, endocrocin, from an mdphdelta strain suggests that mdph might encode a decarboxylase. furthermore, by replacing the promoter of mdpa (a putative aflj homolog) and ... | 2010 | 20139316 |
| genotoxic activity of eucalyptus globulus essential oil in aspergillus nidulans diploid cells. | eucalyptus globulus essential oil was evaluated for its genotoxic potential using a somatic segregation assay and a diploid strain of the fungus aspergillus nidulans, heterozygous for nutritional and conidia color markers. the main compounds of the current essential oil sample were eucalyptol (49.0 %), alpha-pinene (8.9), beta-pinene (1.5), globulol (6.9), alpha-eudesmol (1.12), spathulenol (1.42), gamma-cadinene (1.45), trans-beta-elemenone (1.23) and aromandendrene (2.3), totaling 74 % of oil. ... | 2010 | 20140715 |
| asperfuranone from aspergillus nidulans inhibits proliferation of human non-small cell lung cancer a549 cells via blocking cell cycle progression and inducing apoptosis. | asperfuranone, a novel compound of genomic mining in aspergillus nidulans, was investigated for its anti-proliferative activity in human non-small cell lung cancer a549 cells. to identity the anti-cancer mechanism of asperfuranone, we assayed its effect on apoptosis, cell cycle distribution, and levels of p53, p21 waf1/cip1, fas/apo-1 receptor and fas ligand. enzyme-linked immunosorbent assay showed that the g0/g1 phase arrest might be due to p53-dependent induction of p21 waf1/cip1. an enhancem ... | 2010 | 20148857 |
| the conserved and divergent roles of carbonic anhydrases in the filamentous fungi aspergillus fumigatus and aspergillus nidulans. | carbon dioxide (co(2)) and its hydration product bicarbonate (hco(3)(-)) are essential molecules in various physiological processes of all living organisms. the reversible interconversion between co(2) and hco(3)(-) is in equilibrium. this reaction is slow without catalyst, but can be rapidly facilitated by zn(2+)-metalloenzymes named carbonic anhydrases (cas). to gain an insight into the function of multiple clades of fungal ca, we chose to investigate the filamentous fungi aspergillus fumigatu ... | 2010 | 20149101 |
| overexpression of bacterial ethylene-forming enzyme gene in trichoderma reesei enhanced the production of ethylene. | in order to efficiently utilize natural cellulose materials to produce ethylene, three expression vectors containing the ethylene-forming enzyme (efe) gene from pseudomonas syringae pv. glycinea were constructed. the target gene was respectively controlled by different promoters: cbh i promoter from trichoderma reesei cellobiohydrolases i gene, gpd promoter from aspergillus nidulans glyceraldehyde-3-phosphate dehydrogenase gene and pgk i promoter from t. reesei 3-phosphoglycerate kinase i gene. ... | 2010 | 20150979 |
| expression and purification of a functional uric acid-xanthine transporter (uapa). | the nucleobase-ascorbate transporters (nats) family includes carriers with fundamental functions in uptake of key cellular metabolites, such as uric acid or vitamin c. the best studied example of a nat transporter is the uric acid-xanthine permease (uapa) from the model ascomycete aspergillus nidulans. detailed genetic and biochemical analyses have revealed much about the mechanism of action of this protein; however, the difficulties associated with handling eukaryotic membrane proteins have lim ... | 2010 | 20153431 |
| evaluation of performance and community dynamics of microorganisms during treatment of distillery spent wash in a three stage bioreactor. | the ability of emericella nidulans var. lata, neurospora intermedia and bacillus sp. to treat distillery spent wash in a three stage bioreactor was investigated. process parameters were optimized in shake flask cultures with the individual strains before treatment of the effluent in a 15-l bioreactor. treatment was first carried out by the fungi followed by bacteria. the treated effluent showed significant reduction in color (82%) and cod (93%) after 30 h. metabolites formed after degradation of ... | 2010 | 20156685 |
| metabolic and developmental effects resulting from deletion of the cita gene encoding citrate synthase in aspergillus nidulans. | citrate synthase is a central activity in carbon metabolism. it is required for the tricarboxylic acid (tca) cycle, respiration, and the glyoxylate cycle. in saccharomyces cerevisiae and arabidopsis thaliana, there are mitochondrial and peroxisomal isoforms encoded by separate genes, while in aspergillus nidulans, a single gene, cita, encodes a protein with predicted mitochondrial and peroxisomal targeting sequences (pts). deletion of cita results in poor growth on glucose but not on derepressin ... | 2010 | 20173036 |
| molecular genetic analysis of the orsellinic acid/f9775 gene cluster of aspergillus nidulans. | f-9775a and f-9775b are cathepsin k inhibitors that arise from a chromatin remodelling deletant strain of aspergillus nidulans. a polyketide synthase gene has been determined to be responsible for their formation and for the simpler, archetypical polyketide orsellinic acid. we have discovered simple culture conditions that result in the production of the three compounds, and this facilitates analysis of the genes responsible for their synthesis. we have now analysed the f9775/orsellinic acid gen ... | 2010 | 20174687 |
| regulation of septum formation by the bud3-rho4 gtpase module in aspergillus nidulans. | the ability of fungi to generate polarized cells with a variety of shapes likely reflects precise temporal and spatial control over the formation of polarity axes. the bud site selection system of saccharomyces cerevisiae represents the best-understood example of such a morphogenetic regulatory system. however, the extent to which this system is conserved in the highly polarized filamentous fungi remains unknown. here, we describe the functional characterization and localization of the aspergill ... | 2010 | 20176976 |
| dynamic elements at both cytoplasmically and extracellularly facing sides of the uapa transporter selectively control the accessibility of substrates to their translocation pathway. | in the uapa uric acid-xanthine permease of aspergillus nidulans, subtle interactions between key residues of the putative substrate binding pocket, located in the tms8-tms9 loop (where tms is transmembrane segment), and a specificity filter, implicating residues in tms12 and the tms1-tms2 loop, are critical for function and specificity. by using a strain lacking all transporters involved in adenine uptake (deltaazga deltafcyb deltauapc) and carrying a mutation that partially inactivates the uapa ... | 2010 | 20188741 |
| mitotic crossing-over induced by two commercial herbicides in diploid strains of the fungus aspergillus nidulans. | some herbicides are suspected of promoting teratogenic, carcinogenic and mutagenic events. detection of induced mitotic crossing-over has proven to be an indirect way of testing the carcinogenic properties of suspicious substances, because mitotic crossing-over is involved in the multistep process of carcinogenesis. we examined mitotic crossing-over induced by two commercial herbicides (diuron and trifluralin) in diploid strains of aspergillus nidulans based on the homozygotization index. low do ... | 2010 | 20198578 |
| proteome analysis of fungal and bacterial involvement in leaf litter decomposition. | fungi and bacteria are key players in the decomposition of leaf litter, but their individual contributions to the process and their interactions are still poorly known. we combined semi-quantitative proteome analyses (1-d page-lc-ms/ms) with qualitative and quantitative analyses of extracellular degradative enzyme activities to unravel the respective roles of a fungus and a bacterium during litter decomposition. two model organisms, a mesophilic gram-negative bacterium (pectobacterium carotovoru ... | 2010 | 20198641 |
| aspergillus nidulans udp-glucose-4-epimerase ugea has multiple roles in wall architecture, hyphal morphogenesis, and asexual development. | aspergillus nidulans udp-glucose-4-epimerase ugea interconverts udp-glucose and udp-galactose and participates in galactose metabolism. the sugar moiety of udp-galactose is predominantly found as galactopyranose (galp, the six-membered ring form), which is the substrate for udp-galactopyranose mutase (encoded by ugma) to generate udp-galactofuranose (galf, the five-membered ring form) that is found in fungal walls. in a. fumigatus, galf residues appear to be important for virulence. the a. nidul ... | 2010 | 20211750 |
| involvement of transposon-like elements in penicillin gene cluster regulation. | subtelomeric secondary metabolite (sm) gene clusters are frequently surrounded by dna repeats and transposon-like elements. the aspergillus nidulans penicillin cluster, 30kb from the telomere of chromosome vi, is bordered by such elements. deletions of penicillin telomere proximal and distal border regions resulted in decreased penicillin production. a 3.7kb distal region called pbia, consisting of the putative transposable element dna-2, was examined further where its replacement by a pyrg mark ... | 2010 | 20219692 |
| effect of light on growth, intracellular and extracellular pigment production by five pigment-producing filamentous fungi in synthetic medium. | the competence of the living creatures to sense and respond to light is well known. the effect of darkness and different color light quality on biomass, extracellular and intracellular pigment yield of five potent pigment producers monascus purpureus, isaria farinosa, emericella nidulans, fusarium verticillioides and penicillium purpurogenum, with different color shades such as red, pink, reddish brown and yellow, were investigated. incubation in total darkness increased the biomass, extracellul ... | 2010 | 20226375 |
| distinct roles for caf1, ccr4, edc3 and cuta in the co-ordination of transcript deadenylation, decapping and p-body formation in aspergillus nidulans. | transcript degradation is a key step in gene regulation. in eukaryotes, mrna decay is generally initiated by removal of the poly(a) tail mediated by the ccr4-caf1-not complex. deadenylated transcripts are then rapidly degraded, primarily via the decapping-dependent pathway. components of this pathway can be localized into highly dynamic cytoplasmic foci, the mrna processing (p)-bodies. we have undertaken confocal fluorescence microscopy to monitor p-bodies in aspergillus nidulans. as in other or ... | 2010 | 20233300 |
| deletion of the ring-finger peroxin 2 gene in aspergillus nidulans does not affect meiotic development. | peroxins are required for protein import into peroxisomes as well as for peroxisome biogenesis and proliferation. loss-of-function mutations in genes for the ring-finger peroxins pex2, pex10 and pex12 lead to a specific block in meiosis in the ascomycete podospora anserina. however, loss of protein import into peroxisomes does not result in this meiotic defect. therefore, it has been suggested that these peroxins have a specific function required for meiosis. to determine whether this role is co ... | 2010 | 20236327 |
| novel insights into the functional role of three protein arginine methyltransferases in aspergillus nidulans. | protein arginine methylation has been implicated in different cellular processes including transcriptional regulation by the modification of histone proteins. here we demonstrate significant in vitro activities and multifaceted specificities of aspergillus protein arginine methyltransferases (prmts) and we provide evidence for a role of protein methylation in mechanisms of oxidative stress response. we have isolated all three aspergillus prmts from fungal extracts and could assign significant hi ... | 2010 | 20338257 |
| interaction of the aspergillus nidulans microtubule-organizing center (mtoc) component apsb with gamma-tubulin and evidence for a role of a subclass of peroxisomes in the formation of septal mtocs. | peroxisomes are a diverse class of organelles involved in different physiological processes in eukaryotic cells. although proteins imported into peroxisomes carry a peroxisomal targeting sequence at the c terminus (pts1) or an alternative one close to the n terminus (pts2), the protein content of peroxisomes varies drastically. here we suggest a new class of peroxisomes involved in microtubule (mt) formation. eukaryotic cells assemble mts from distinct points in the cell. in the fungus aspergill ... | 2010 | 20348383 |
| aspergillus nidulans contains six possible fatty acyl-coa synthetases with faab being the major synthetase for fatty acid degradation. | aspergillus nidulans can use a variety of fatty acids as sole carbon and energy sources via its peroxisomal and mitochondrial beta-oxidation pathways. prior to channelling the fatty acids into beta-oxidation, they need to be activated to their acyl-coa derivates. analysis of the genome sequence identified a number of possible fatty acyl-coa synthetases (fata, fatb, fatc, fatd, faaa and faab). faab was found to be the major long-chain synthetase for fatty acid degradation. faab was shown to local ... | 2010 | 20354844 |
| a septin from the filamentous fungus a. nidulans induces atypical pseudohyphae in the budding yeast s. cerevisiae. | septins, novel cytoskeletal proteins, form rings at the bases of emerging round buds in yeasts and at the bases of emerging elongated hyphal initials in filamentous fungi. | 2010 | 20360845 |
| gdp-mannose transporter paralogues play distinct roles in polarized growth of aspergillus nidulans. | gdp-mannose transporters (gmt) carry gdp-mannose nucleotide sugars from the cytosol across the golgi apparatus membrane for use as substrates in protein glycosylation in plants, animals and fungi. genomes of some fungal species, such as the yeast saccharomyces cerevisiae, contain only one gene encoding a gmt, while others, including aspergillus nidulans, contain two (gmta and gmtb). we previously showed that cell wall integrity and normal hyphal morphogenesis in a. nidulans depend upon the funct ... | 2010 | 20361498 |
| characterization of aspergillus nidulans didb did2, a non-essential component of the multivesicular body pathway. | escrt-iii heteropolymers mediate membrane protein cargo sorting into multivesicular endosomes for subsequent vacuolar degradation. we studied the localization of largely uncharacterized aspergillus nidulans escrt-iii using its key structural component vps32 and the 'associated' component didb(did2). vps32-gfp localizes to motile early endosomes as reported, but predominates in aggregates often associated with vacuoles due to inability to dissociate from endosomes. didb(did)(2) regulating vps4 (t ... | 2010 | 20362686 |
| single-step affinity purification for fungal proteomics. | a single-step protein affinity purification protocol using aspergillus nidulans is described. detailed protocols for cell breakage, affinity purification, and depending on the application, methods for protein release from affinity beads are provided. examples defining the utility of the approaches, which should be widely applicable, are included. | 2010 | 20363899 |