Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| persistent immunoglobulin deficiency after prednisolone and antiepileptic therapy in a c2 deficient patient with lupus-like syndrome. | a homozygous c2 deficient patient with a lupus-like syndrome developed hypogammaglobulinemia soon after treatment with prednisolone together with phenytoin, replaced subsequently by carbamazepine. she suffered from recurrent chest infections and her lupus symptoms continued unabated. in vitro tests of immunoglobulin production by her epstein barr virus transformed b cells showed typical patterns of reduced iga and igg production seen in common variable hypogammaglobulinemia. an opsonisation defe ... | 1984 | 6520837 |
| gamma-irradiation induces mutation in ataxia-telangiectasia lymphoblastoid cells. | ataxia-telangiectasia (at) cells are hypersensitive to the lethal effect of gamma-rays, whereas little or no gamma-ray induced mutation has been observed. in this work, exposure to gamma-rays of an epstein-barr virus-transformed at lymphoblastoid cell line, gm2783, resulted in a clear dose-dependent increase of mutation for 6-thioguanine resistance. | 1984 | 6526217 |
| [cancer of the nasopharynx in morocco: epidemiological approach]. | between 1 april 1980 and 30 septembre 1983, 412 cases of nasopharyngeal carcinoma were seen at the bergonié cancer centre in casablanca (the sole centre in the country), representing 4.76% of all malignant tumours diagnosed in that period. this percentage is low, since the majority of these tumours are not diagnosed outside the big towns but indicates that morocco is an area of moderate incidence for this cancer. we have studied the epidemiological aspects of our series; there is a predominance ... | 1984 | 6536623 |
| the use of acyclovir in children. | acyclovir represents a significant advance in the treatment of herpes simplex and varicella zoster virus infections and appears to be safe and well-tolerated. physicians now have several drugs to choose from in the treatment of these infections. hopefully there will be other antiviral chemotherapeutic agents that will be effective for therapy of cytomegalovirus and epstein-barr virus infections. coincident with the development of antivirals, continued progress in the area of rapid viral diagnosi ... | 1984 | 6540867 |
| human monoclonal antibody to tumor-associated ganglioside gd2: suppressed growth of human melanoma in nude mice. | human igm kappa monoclonal antibody (anti-gd2) to a tumor antigen, ganglioside gd2, has been produced by epstein-barr virus-transformed human b lymphoblasts. in the present study, we demonstrated the anti-tumor effects of passively administered anti-gd2 against an ascites-form human melanoma cell line, m14-a, transplanted into athymic nude mice. m14-a expresses gd2 in vivo. cells (5 x 10(5) were inoculated intraperitoneally (i.p.) or subcutaneously (s.c.) into cd-1 nude mice that had received i. ... | 1984 | 6542067 |
| [x-linked lymphoproliferative syndrome]. | this report deals with the case history of a 21/2-year old turkish boy, who died from an overwhelming mononucleosis infectiosa. the parents, first cousins, lost already two sons and a daughter. according to the parents, the symptoms of these children resembled those of this boy. in the patient, described here, an acquired immunodeficiency was found, probably induced by the epstein-barr virus on the basis of a genetic susceptibility for this virus. the family history, the clinical picture, togeth ... | 1984 | 6543257 |
| mutant hla-a2 antigens as restricting elements for virus-specific cytotoxic t cells. | mutants of the eb virus-transformed cell line t5-1 (hla-a1, 2; b8, 27), bearing well-characterized alterations in hla-a2 antigen expression and unable to bind the hla-a2-specific monoclonal antibody bb7.2, have been tested for their susceptibility to eb virus-specific cytolysis using effector t-cell preparations functionally restricted through relevant hla antigens. initial experiments first confirmed that the parent line t5-1 was susceptible to cytolysis by both "common" a2-restricted and b27-r ... | 1984 | 6329950 |
| antibody-dependent cellular cytotoxicity and disease course in north american patients with nasopharyngeal carcinoma: a prospective study. | it has previously been reported that antibody-dependent cellular cytotoxicity (adcc) levels determined at diagnosis against epstein-barr virus (ebv)-specific membrane antigens (ma) are predictive of disease course following therapy in patients with nasopharyngeal carcinoma (npc). in general, survival was significantly shorter following therapy in patients with low adcc titers at diagnosis versus those with high titers. to examine this further, a prospective study on north americans with differen ... | 1984 | 6329967 |
| expression of hla-dr antigens in nasopharyngeal carcinoma: an immunohistological analysis of the tumour cells and infiltrating lymphocytes. | a panel of conventional and monoclonal antibodies was used to examine the immunohistological characteristics of malignant epithelial cells and infiltrating lymphocytes in frozen sections of nasopharyngeal carcinoma (npc) biopsies from 10 tunisian patients. three main categories of cells were identified. (1) tumour cells which were positive for epstein-barr nuclear antigen, hla-abc and keratin determinants. in most samples, the tumour cells also expressed variable amounts of hla-dr antigens. the ... | 1984 | 6329970 |
| isolation and characterization of an ebv superinfection-enhanced, host-cell-coded, 53,000-dalton protein. | microsomal membrane fraction proteins with enhanced synthesis after p3hr-1 epstein-barr virus (ebv) superinfection of raji cells were identified with [35s]-methionine labeling and sds-page. one 53,000-dalton protein, which was found in both the microsomal membrane and cytosol fractions, was purified by ion-exchange chromatography, and specific rabbit antisera were prepared to it. this protein was found to be present in raji cells, but its expression was enhanced after p3hr-1 ebv superinfection. ... | 1984 | 6329996 |
| differential inhibitory effects of retinoic acid on epstein-barr virus induction in producer and nonproducer cell lines. | the effects of retinoic acid on epstein-barr virus (ebv) antigen induction by 12-o-tetradecanoyl-phorbol-13-acetate, 5-iodo-2'-deoxyuridine, and n-butyrate and by combinations of them were examined in two virus-producer and two nonproducer cell lines. irrespective of the inducers used, the induction was markedly inhibited in the nonproducer lines and only weakly inhibited in the producer lines. the possible sites of action of retinoic acid are discussed. | 1984 | 6329997 |
| production of interleukin 2 containing growth medium for cloning of human t lymphocytes. | culture conditions which would enhance production of interleukin 2 (il-2) from pha-stimulated peripheral blood mononuclear cells (pbm) were studied. one thousand rads. irradiation of the cells was found to alter the kinetics and to increase the production of il-2. addition of indomethacin or irradiated ebv-transformed b lymphocytes to the cultures, removal of adherent cells, and 18 h preincubation of the cells before pha stimulation had an additive enhancing effect on the il-2 production. follow ... | 1984 | 6330207 |
| structural organization of human herpesvirus dna molecules. | the herpesviruses are among the largest and most complex of all dna viruses, and their genomes display an astonishing diversity in size, structure, and organization. in 1974, the features of large inverted repeats and structural isomerization were first discovered, and these proved to be characteristic properties of many herpesvirus genomes. since then, research using the powerful techniques of modern molecular biology has revealed a great deal of comparative structural information about the arr ... | 1984 | 6330219 |
| speculations on the viral etiology of acquired immune deficiency syndrome and kaposi's sarcoma. | the acquired immune deficiency syndrome (aids) appeared in the united states in late 1978 and has spread at an epidemic rate through the four major coastal cities of this country. the disease appears to show the same epidemiologic distribution as hepatitis b virus infection, and for this reason, most investigators feel that this new disease is caused by a blood-borne sexually transmitted virus. a number of viral agents have been suggested as the cause of aids, but to date, no virus has been cons ... | 1984 | 6330223 |
| transforming functions associated with epstein-barr virus. | epstein-barr virus (ebv) transforms the human b-lymphocytes it infects into lymphoblasts that are competent to proliferate indefinitely in tissue culture [1]. the dividing transformed lymphoblasts carry multiple and complete copies of ebv dna as plasmids [1]. no viral functions that are necessary for ebv-induced transformation have been identified and characterized to date. we have developed an assay that aids in the identification of viral functions needed to maintain the viral plasmid in trans ... | 1984 | 6330229 |
| benign and malignant disease caused by ebv. | epstein-barr virus (ebv) causes infectious mononucleosis as a primary disease. the virus infects more than 90% of the average population and persists lifelong in peripheral b-lymphocytes. the virus is produced in the parotid gland and spread via the oral route. serology suggests that the epstein-barr virus might be involved in the causation of two neoplastic diseases of humans: african burkitt's lymphoma and nasopharyngeal carcinoma. whereas the development of the lymphoma has an even better lin ... | 1984 | 6330230 |
| fatal epstein-barr virus-induced lymphoproliferation complicating acute lymphoblastic leukemia. | 1984 | 6330336 | |
| acquired immune deficiency disease after three years. the unsolved riddle. | 1984 | 6330451 | |
| clonality of ebv-induced lymphoproliferative diseases in immune-deficient patients. | 1984 | 6330551 | |
| case records of the massachusetts general hospital. weekly clinicopathological exercises. case 31-1984. a three-year-old boy with fever, pancytopenia, and multisystem failure. | 1984 | 6330555 | |
| epstein-barr virus in epithelium. | 1984 | 6330572 | |
| viral infections in immunosuppressed patients. | 1984 | 6330629 | |
| immunodeficiency as a factor in lymphomagenesis. | the human immune system has evolved multiple cellular and humoral defense mechanisms against the lymphotropic virus, ebv. nk cells, suppressor t-cells, cytotoxic k-cells, memory t-cells, and humoral immune responses usually subdue the virus into latency. individuals with immune deficiency are at great risk of developing immunoregulatory disturbances and lymphoproliferative diseases when confronted by ebv. the infection of b-cells by ebv provokes a marked activation of immunoregulatory t-cells an ... | 1984 | 6330665 |
| homology between two ebv early genes and hsv ribonucleotide reductase and 38k genes. | computer-matching of amino acid sequences predicted from the complete ebv dna sequence against the known hsv gene sequences has revealed significant homology between two ebv reading frames and the hsv1 and hsv2 140k and 38k proteins which are associated with ribonucleotide reductase activity. the two genes are arranged tandemly as in hsv though it appears that, unlike hsv, the two mrnas are not 3' co-terminal. we have mapped two promoters predicted from the dna sequence for these genes and shown ... | 1984 | 6330697 |
| epstein-barr virus-associated syndromes: a critical reexamination. | 1984 | 6330703 | |
| the spectrum of ebv infections. | 1984 | 6330710 | |
| induction of gamma-interferon activity by elevated temperatures in human b-lymphoblastoid cell lines. | when the human b-lymphoblastoid cell lines gm3299 and gm4408 were heat shocked, elevated levels of antiviral activity were detected. this antiviral activity has been characterized as the result of de novo synthesis of interferon (ifn) gamma. levels of antiviral activity as high as 20,000 units/ml per 10(6) cells were found after heat treatment. by using anti-ifn-alpha and anti-ifn-gamma sera, it was shown that the high antiviral activity in gm3299 after heat shock is due to a synergistic effect ... | 1984 | 6330733 |
| detection of an epstein-barr virus-associated membrane antigen in epstein-barr virus-transformed nonproducer cells by leukocyte migration inhibition and blocking antibody. | soluble membrane fractions derived from raji cells trigger lymphocytes of epstein-barr virus (ebv)-seropositive, but not ebv-seronegative, individuals to release a lymphokine that inhibits leukocyte migration. the reaction can be blocked by the sera of patients with ebv-dna-carrying tumors, burkitt lymphoma, or nasopharyngeal carcinoma. absorption of these sera with ebv-positive, but not ebv-negative, cells abrogates their blocking activity. these findings suggest that the antigen responsible fo ... | 1984 | 6330746 |
| identification and mapping of polypeptides encoded by the p3hr-1 strain of epstein-barr virus. | the epstein-barr virus (ebv)-specified polypeptides induced upon viral replication in the p3hr-1 cell line have been examined by immunoprecipitation with a high-titer human anti-ebv serum. twenty-five predominant polypeptides were identified in cell extracts, whereas 18 polypeptides were precipitated from cell-free translation reactions directed by total mrna. hybrid selection of mrna to the bamhi dna clones of the ebv genome and immunoprecipitation of the corresponding cell-free translation pro ... | 1984 | 6330747 |
| epstein-barr virus-induced lymphoblastoid cell lines derived from the peripheral blood of patients with x-linked agammaglobulinemia can secrete igm. | we have established lymphoblastoid cell lines (lcl) from the peripheral blood of three individuals with x-linked agammaglobulinemia as well as three of their immunodeficient first-degree relatives. lines could be induced with epstein-barr virus only when t lymphocytes were depleted from total mononuclear leukocytes. the lcls derived from xla patients expressed characteristics of igm-secreting plasmacytes, including intense cytoplasmic fluorescence after staining with anti-mu, easily detectable a ... | 1984 | 6327761 |
| abnormally elevated frequency of epstein-barr virus-infected b cells in the blood of patients with rheumatoid arthritis. | patients with rheumatoid arthritis (ra) are known to have in vitro regulatory t cell abnormalities relating to epstein-barr virus (ebv). in this report, we asked whether patients with ra have more circulating ebv-infected b cells than normals. to address this question, we determined the frequency of spontaneously transforming b cells in the peripheral blood of 18 normals, 15 patients with ra, and 8 patients with systemic lupus erythematosus (sle). the mean frequency of spontaneously transforming ... | 1984 | 6327772 |
| complement-dependent, epstein-barr virus-neutralizing antibody appearing early in the sera of patients with infectious mononucleosis. | the first serum specimens obtained after onset of illness and those obtained later from patients with infectious mononucleosis were examined for neutralization of p3hr-1-derived epstein-barr virus (ebv). without complement, viral neutralization indices of sera obtained soon after onset were all less than 0.2, and those of sera obtained later were less than 0.5. in contrast, sera from ebv-seropositive, healthy adults had neutralization indices of 0.45-1.0. sera from ebv-seronegative adults showed ... | 1984 | 6327851 |
| accessory cell function of human b cells. i. production of both interleukin 1-like activity and an interleukin 1 inhibitory factor by an ebv-transformed human b cell line. | in the present paper we report that the roha -9 cell line, an epstein-barr virus (ebv)-transformed human b cell line with accessory cell capabilities, constitutively secretes a soluble factor with the biochemical and biological characteristics of human monocyte-derived il-1. the il-1 derived from roha -9 augmented murine thymocyte proliferation and enhanced the proliferative response of human t lymphocytes to concanavalin a (con a). the roha -9-derived il-1 activity eluted from sephacryl s-200 i ... | 1984 | 6327874 |
| comparative immunogenicity studies on epstein-barr virus membrane antigen (ma) gp340 with novel adjuvants in mice, rabbits, and cotton-top tamarins. | the effectiveness of immunisation of mice, rabbits, and cotton-top tamarins with small amounts of eb virus ma glycoprotein gp340 , incorporated into artificial liposomes, has been compared using various routes of injection with or without additional adjuvants. liposomes containing gp340 gave specific high titre antibodies after i.p. or i.v. administration, and the addition of lipid a to the liposomes resulted in a significant enhancement of the response. antibodies generated by the above procedu ... | 1984 | 6327902 |
| expression in cos-1 cells of epstein-barr virus nuclear antigen from a complete gene and a deleted gene. | in a previous study the bamhi-k fragment of epstein-barr virus dna was shown to induce a nuclear antigen, epstein-barr virus nuclear antigen (ebna), when cotransfected with the herpes simplex virus thymidine kinase gene into mouse ltk- cells. we have now inserted the bamhi-k fragment and a bamhi/hindiii subfragment, i1f , into shuttle vectors containing the origin of replication of simian virus 40. these plasmids have been introduced into cos-1, which are monkey kidney cells transformed by an or ... | 1984 | 6328012 |
| structure of defective dna molecules in epstein-barr virus preparations from p3hr-1 cells. | epstein-barr virus (ebv), isolated from p3hr-1 cells, induces early antigen and viral capsid antigen upon infection of human b-lymphoblasts. the strong early antigen- and viral capsid antigen-inducing activity is only observed in p3hr-1 virus preparations harboring particles with defective genomes, suggesting that this biological activity is directly associated with the defective dna population. after infection of ebv genome-carrying raji or ebv genome-negative bjab cells, defective genomes of p ... | 1984 | 6328039 |
| herpesvirus infections in the acquired immune deficiency syndrome. | herpesvirus infections were studied in persons with or at risk for the acquired immune deficiency syndrome (aids). the infections diagnosed were as follows: patients with aids, cytomegalovirus (cmv) in 34 of 34, epstein-barr virus (ebv) in 33 of 34, herpes simplex viruses (hsv) in eight of 34, and varicella zoster virus in four of 34; patients with chronic lymphadenopathy syndrome, cmv in eight of nine and ebv in nine of nine; in healthy homosexual men, cmv in five of 13 and ebv in seven of eigh ... | 1984 | 6328055 |
| analysis of cytomegalovirus and epstein-barr virus antibody responses in treated hemophiliacs. implications for the study of acquired immune deficiency syndrome. | one hundred hemophiliacs were studied for serological evidence of infection with cytomegalovirus (cmv), epstein-barr virus (ebv), and hepatitis b virus. ninety-eight percent had markers of hepatitis b infection, while 69% had antibody to ebv and only 42% had antibody to cmv, suggesting that factor viii preparations do not transmit ebv and cmv efficiently. seventy-one percent of those seropositive to ebv had an antibody pattern suggestive of active infection, as compared with 23% of healthy young ... | 1984 | 6328057 |
| hematologic and immunologic responses in common marmosets (callithrix jacchus) infected with plasmodium knowlesi and epstein-barr virus. | the hematologic and immunologic responses to infection with either the epstein-barr virus alone or infection with epstein-barr virus and plasmodium knowlesi were studied using common marmosets (callithrix jacchus). the assays performed included complete blood cell counts, determinations of natural killer cell activity, and determinations of antibody titers to epstein-barr virus early antigen, virus capsid antigen and the nuclear antigen. while no animal showed signs of lymphoproliferative diseas ... | 1984 | 6328114 |
| dna methylation and expression of hla-dr alpha. | b-cell lines established from two individuals with t-cell acute lymphocytic leukemia (t-all) express hla-dr antigens, whereas the isogenic t-cells do not. the lack of expression correlates with a lack of detectable hla-dr mrna. all of the dr alpha dna sequences detected by a cloned dr alpha cdna probe are contained in a bglii fragment which varies slightly in size (4.0 to 4.8 kilobases) from one individual to another. in dna from the t-cells not expressing dr alpha mrna, all of the potential hpa ... | 1984 | 6328275 |
| epstein-barr virus transforms precursor b cells even before immunoglobulin gene rearrangements. | the very early stages of the human b-cell differentiation pathway are poorly understood, primarily because of the lack of appropriate permanent cell lines. epstein-barr virus (ebv) is a putative human oncogenic virus which transforms human b cells in vitro into continuously proliferating cells. it has been believed that ebv transforms mature b cells, but recently, transformation of immature pre-b-cell lines has been reported, suggesting that ebv might also transform cells much earlier in the b-c ... | 1984 | 6328311 |
| a cis-acting element from the epstein-barr viral genome that permits stable replication of recombinant plasmids in latently infected cells. | the epstein-barr viral (ebv) genome of approximately equal to 170 kilobase pairs (kbp) is maintained as a plasmid in human b lymphoblasts transformed by the virus. we have identified a cis-acting element within 1.8 kbp of the viral genome that allows recombinant plasmids carrying it to be selected at high frequency and maintained as plasmids in cells latently infected by ebv. this functional element(s) requires a segment of dna at least 800 bp and at most 1800 bp long, which contains a family of ... | 1984 | 6328526 |
| [recent aspects of epstein-barr virus infections]. | epstein-barr virus (ebv) is regularly associated with burkitt's lymphoma (nasopharyngeal carcinoma) and has been extensively documented as causal in infectious mononucleosis. recently it has become evident that this virus is associated or handled differently in patients with a variety of immunological disorders. if the mechanisms that normally limit the effects of ebv infection are blunted, as in rheumatoid arthritis, antibody responses to ebv induced cellular antigens are significantly higher t ... | 1984 | 6328644 |
| adaptation of lymphadenopathy associated virus (lav) to replication in ebv-transformed b lymphoblastoid cell lines. | a strain of lymphadenopathy associated retrovirus ( lav ) passaged in vitro was used to infect a lymphoblastoid cell line obtained by transformation with epstein-barr virus of b lymphocytes from a healthy donor. the virus produced from this line (b- lav ) was also able to grow at a high rate in some other lymphoblastoid lines and in a burkitt lymphoma line. this adapted strain retained the biochemical, ultrastructural, and antigenic characteristics of the original strain, as well as its tropism ... | 1984 | 6328661 |
| hla-dr antigens and the antibody response against epstein-barr virus. | antibodies against epstein-barr virus (ebv) antigens, i.e. the viral capsid antigen (vca) and the epstein-barr nuclear antigen (ebna), were determined in two independent populations with known hla-dr phenotypes. the first population consisted of 151 patients with rheumatoid arthritis; the second one of 88 healthy parents of leukemic children. although the group of patients with rheumatoid arthritis differed significantly in the frequency of 4 dr antigens from the second group, both groups had th ... | 1984 | 6328695 |
| [virologic-serologic diagnosis of nasopharyngeal cancer]. | 1984 | 6328701 | |
| gianotti-crosti syndrome. a review of ten cases not associated with hepatitis b. | studies of data from ten cases of infantile acrodermatitis and from eight cases reported in the north american literature disclose distinctive papular dermatosis of the face and extremities, often related to virus infection. none of our eight patients who were tested had evidence of hepatitis b infections, although transaminase values were elevated in two. all five patients who were tested had lymphocytosis. six patients had antecedent upper respiratory tract symptoms. data from our cases and fr ... | 1984 | 6329107 |
| late-onset, warfarin-caused necrosis occurring in a patient with infectious mononucleosis. | a 25-year-old man with klinefelter's syndrome and recurrent thromboplebitis , for which he had been receiving long-term warfarin sodium therapy, had bilateral ecchymoses on the hips coincident with serologically confirmed epstein-barr virus-caused mononucleosis. biopsy specimens taken from the hip lesions showed microscopic findings consistent with a diagnosis of warfarin necrosis. direct immunofluorescence microscopy disclosed vessel-wall deposition of igm and heavy upper-dermal deposition of i ... | 1984 | 6329108 |
| [a specific histological marker of undifferentiated carcinoma of the nasopharynx. the acif (anti-complement immunofluorescence) test]. | the relationship between e.b.v. infection and nasopharyngeal carcinomas can be used as a marker of these tumors (a.c.i.f. test). the method reveals the nuclear antigen (e.b.n.a.) by anti complement immunofluorescence. it was applied to 102 patients with n.p.c. and was found positive only in undifferentiated carcinoma (u.c.n.t.). this "marker" can be useful in search of primary from lymph node metastasis. | 1984 | 6329239 |
| enzyme studies on epstein-barr virus-transformed lymphoid cell lines from wolman's disease. lipases, cholesterol esterase and 4-methylumbelliferyl acyl ester hydrolases. | (1) in lymphoid cell lines established by epstein-barr virus transformation of b-lymphocytes from normal subjects there exist two lipases hydrolysing triolein (the first one with acid optimum ph and the other one with alkaline optimum ph) and one cholesterol esterase (with acidic optimum ph). the acid triolein lipase (optimum ph 3.75-4.0) and the acid cholesterol esterase are activated by taurocholate (optimal concentration between 1 and 2.5 g/l) whereas alkaline triolein-lipase is inhibited by ... | 1984 | 6329310 |
| natural killer cell sensitivity of human lymphoid lines of b-cell origin does not correlate with tumorigenicity or with the expression of certain differentiation markers. | human b-cell lines derived from normal donors (lcl) or from burkitt lymphomas (bl) were compared for their sensitivity to natural (nk) and interferon (ifn)-activated (iak) cytotoxicity, mediated by effector cells from normal human blood. in four cases, a bl and an lcl line were derived from the same donor and had been kept in culture for the same period of time. the bl series included both epstein-barr virus (ebv)-carrying and ebv-negative lymphoma lines. the latter were compared with their own ... | 1984 | 6329520 |
| t-lymphocyte subset interactions in the cell-mediated immune response to epstein-barr virus. | the t-lymphocyte subset interactions in the cell-mediated response to epstein-barr virus (ebv) were studied in an in vitro system in which the ability of t lymphocytes to inhibit outgrowth of autologous ebv-transformed b lymphocytes is assessed. inhibition could be demonstrated within lymphocytes of both the t4 and t8 surface phenotypes. outgrowth inhibition was observed more frequently when the effector t-cell population contained cells of both surface phenotypes. in blocking experiments the ok ... | 1984 | 6329522 |
| plasmodium falciparum products enhance human lymphocyte transformation by epstein-barr virus. | supernatants obtained from the in vitro culture of plasmodium falciparum infected erythrocytes induced prolonged lymphocyte survival in culture for more than 8 weeks in six cultures and permanent cell lines were established in four of these. the cells in the latter showed lymphoblastoid features similar to those seen in parallel cultures to which transforming epstein-barr (eb) virus instead of p. falciparum derived substances had been added. cells from the same donors stimulated with other mitog ... | 1984 | 6329564 |
| herpes virus antigens and antibodies in kidney biopsies and sera of iga glomerulonephritic patients. | antibody titers against 4 viruses of the herpes group have been studied in the sera of 54 patients with iga glomerulonephritis (gn). the incidence of antibodies to herpes simplex virus type-1 (hsv-1), hsv-2, cytomegalovirus (cmv), epstein-barr virus nuclear antigen (ebna), and that of igg antibodies to ebv virus capsid antigen (vca) were similar in patients and in controls. however, elevated antibody titers (greater than or equal to 64) to hsv and cmv occurred more frequently in patients with ig ... | 1984 | 6329570 |
| a complete set of overlapping cosmid clones of m-aba virus derived from nasopharyngeal carcinoma and its similarity to other epstein-barr virus isolates. | dna of the transforming, nondefective epstein-barr virus (ebv) strain m-aba, which is derived from nasopharyngeal carcinoma cells, was cloned as large overlapping pieces into the cosmid phc79 . the termini were cloned from closed circular virus dna molecules out of m-aba cell dna in phage lambda l47 . the large overlapping clones were used to prepare a library of subclones with inserts of 1-15 kb. a detailed restriction enzyme map of m-aba virus dna reveals the close similarity to isolates from ... | 1984 | 6329907 |
| werner's syndrome: proliferation in vitro of clones of cells bearing chromosome translocations. | each of several cultures of werner's syndrome (ws) fibroblasts and lymphoblasts examined was found to be composed of one or several clones of cells with mutated chromosome complements. two "sister" fibroblasts cell lines (fcls) that were derived from a mixture of explants cut from the same ws skin biopsy were found to have completely different rearranged chromosome complements. daily observation of the skin explants from which these two sister fcls were derived revealed not only that no more tha ... | 1984 | 6324581 |
| on the chromosomal sites of epstein-barr virus in burkitt tumor cell lines. | 1984 | 6324583 | |
| epstein-barr virus nuclear antigen-positive nasopharyngeal hybrid cells. | an epithelial-like hybrid cell line was established by cell fusion of 8-azahypoxanthine-resistant epithelial cells (ad-ah) with lymphoblastoid cells (a2l), derived from the human nasopharynx. the nasopharyngeal hybrid cells, designated as a2l/ah, were epstein-barr virus (ebv)-associated nuclear antigen (ebna)-positive by the anticomplement immunofluorescence method. furthermore, the treatment of the hybrid cells with 5-iodo-2' -deoxyuridine (iudr) induced early antigen (ea) and viral capsid anti ... | 1984 | 6324641 |
| spontaneous and induced immunoglobulin secretion by synovial fluid b lymphocytes in rheumatoid arthritis. | the functional properties of b lymphocytes in synovial fluid (sf) from patients with rheumatoid arthritis (ra) were analysed by means of a reverse haemolytic plaque forming cell (pfc) assay. sf mononuclear cells spontaneously secreted igg, but little igm or iga. the sf cells failed to respond to the polyclonal b cell activators pokeweed mitogen (pwm) and epstein-barr virus. however, sf b cells cocultured with autologous t lymphocytes from the blood and stimulated with pwm secreted igg but little ... | 1984 | 6324703 |
| low density lipoprotein receptor mutations in south african homozygous familial hypercholesterolemic patients. | the binding and catabolism of low density lipoprotein (ldl) were studied in cultured skin fibroblasts and/or epstein-barr virus-transformed lymphoblastoid cells from 20 familial hypercholesterolemic (fh) homozygotes of 14 afrikaner kindreds. cells from available heterozygotes were also analyzed. good resolution between the normal, heterozygote, and homozygote groups was obtained on the basis of these assays using either cell type. results obtained with fibroblasts allowed the classification of 1 ... | 1984 | 6324732 |
| regulation of the cellular actin levels in response to changes in the cell density in ataxia telangiectasia lymphoblastoid cells. | the level of actin was found to decrease markedly when ataxia telangiectasia lymphoblastoid cells were stepped from low to high density culture conditions. additionally, as the actin levels decreased the levels of a protein species of 37k daltons increased by orders of magnitude. partial proteolytic digestion of the 37k protein and actin revealed that the primary structures of these proteins were not related. this phenomena was observed in three out of four ataxia telangiectasia lymphoblastoid c ... | 1984 | 6324767 |
| high frequency of beta-hexosaminidase deficiency in lymphoblastoid cell lines. | the activity of seven lysosomal enzymes was determined in 25 lymphoblastoid cell lines. these lines included normal controls transformed with epstein-barr virus, burkitt's lymphomas and other lymphomas with or without ebv genome. four lines were deficient in total beta-hexosaminidase activity. the deficiency was as severe as that of the variant o (sandhoff's disease) of clinical beta-hexosaminidase deficiency. the electrophoretic pattern was also similar to that observed in sandhoff's disease. t ... | 1984 | 6324783 |
| rheumatoid arthritis nuclear antigen and epstein-barr virus. | 1984 | 6324824 | |
| abdominal burkitt-type lymphomas in algeria. | in a previous retrospective analysis from the principal paediatric centres of algeria, burkitt-type lymphomas (bl) were shown to account for around 46.5% of the total childhood non-hodgkin's malignant lymphomas in that country. in the present study, a series of 49 abdominal bl from the paediatric clinic of surgery, mustapha hospital, algiers, has been studied. the age distribution shows a peak between 4 and 5 years of age, and the sex ratio is (m:f) 2.26:1. the disease is characterized by a rapi ... | 1984 | 6324843 |
| sphingomyelinase and nonspecific phosphodiesterase activities in epstein-barr virus-transformed lymphoid cell lines from niemann-pick disease a, b and c. | acid sphingomyelinase activity determined using the natural substrate, [choline-methyl-14c]sphingomyelin, or the chromogenic synthetic analogue, 2-n-(hexadecanoyl)amino-4-nitrophenylphosphorylcholine, was deficient in epstein-barr virus-transformed lymphoid cell lines from niemann-pick disease types a and b. in contrast, lines from niemann-pick disease type c and "sea-blue histiocyte syndrome" showed a sphingomyelinase activity within the normal range. bis(4-methylumbelliferyl)phosphate and bis( ... | 1984 | 6324871 |
| [is there a functional equivalence between abnormalities of the long arm of chromosome 1 and the presence of epstein-barr virus in continuous lines of burkitt's lymphoma?]. | chromosome 1 long arm abnormalities (translocations, partial of complete trisomies) are non-randomly but inconstantly associated with specific translocations involving chromosomes 8, and 2, 14 or 22 in burkitt's lymphomas and leukemias. all nine burkitt's lymphoma cell lines not associated with epstein-barr virus (ebv) were shown to exhibit a chromosome 1 long arm abnormality and were present in only 3 out of 18 ebv positive cell lines. bands 1q23 - 1q24 were involved in ebv-negative cell lines. ... | 1984 | 6324967 |
| the role of calmodulin in the regulation of human lymphocyte activation. | calmodulin (cam) was isolated from normal and from transformed human lymphocytes by affinity chromatography on capp-sepharose 4b, followed by chromatofocusing. in the presence of ca2+, lymphocyte cam migrated as a single protein on 2-de, and was located on the same position as cam extracted from dog brain and rat testis; its mw was 17,500, with a pi of 3.9. in the presence of ca2+, lymphocyte cam stimulated activator-depleted dog brain phosphodiesterase; this effect was inhibited by trifluoperaz ... | 1984 | 6325021 |
| effects of monoclonal antibodies against lymphocyte surface antigens on interleukin 2 excretion by epstein-barr virus-specific human t-cell hybridomas. | monoclonal antibodies were used as probes to study the role of cell surface antigens in the response of epstein-barr virus (ebv)-specific human t-t hybridomas to autologous ebv-infected b lymphoblasts. somatic cell hybrids were generated by fusing ebv-primed peripheral blood t lymphocytes with a mutant clone of the jm human t-lymphoblastoid-cell line. when exposed to autologous ebv-infected b lymphoblasts, the resulting hybrid clones released interleukin 2 into the culture medium. incubation of ... | 1984 | 6325024 |
| production of leukocyte migration inhibitory factor (lif) in human lymphocyte subsets exposed to polyclonal activators. | lymphocyte subsets separated on the basis of nylon-wool adherence and e and ea rosetting, and characterized for the presence of esterase-positive phagocytic cells were investigated for production of leukocyte migration inhibitory factor (lif) in response to polyclonal t- and b-cell activators, pha, cona, pwm, and epstein-barr virus (ebv). in the nylon-passed population only the high avidity e+ea+ cells responded to cona, pha-induced lif production in all e-rosetting subsets. the nylon-adherent e ... | 1984 | 6325026 |
| [acrodermatitis papulosa eruptiva infantum and epstein-barr virus infection]. | a typical gianotti-crosti syndrome was observed in an 18-month-old boy. this infantile papular acrodermatitis was associated with epstein-barr virus but also with b-antigen-negative hepatitis. biological and serological parameters have been followed up during the course of the illness. | 1984 | 6323351 |
| a comparison of rubazyme-m and macria for the detection of rubella-specific igm. | one-hundred and eighty-six carefully selected sera were tested for rubella-specific igm by rubazyme-m (abbott diagnostics) and an m-antibody capture radioimmunoassay (macria). eleven of these sera were from cases of infectious mononucleosis, six of which gave positive results in macria, while one gave a positive result in rubazyme-m. of the remaining 175 sera, 158 gave concordant results whilst 17 sera gave discordant results; these 17 were also tested by serum fractionation. problems were encou ... | 1984 | 6323513 |
| an immunoperoxidase assay for the detection of specific iga antibody in epstein-barr virus infections. | a technique using indirect immunoperoxidase antibody was developed for the detection of specific serum iga antibody to epstein-barr virus capsid antigen and early antigen. the iga technique was compared with an immunofluorescence antibody method. epstein-barr virus iga antibody against viral capsid antigen was detected in all nine patients with epstein-barr virus associated undifferentiated nasopharyngeal carcinoma, in 13 (72.2%) of 18 patients with infectious mononucleosis, in 21 (28.3%) of 74 ... | 1984 | 6323549 |
| enzyme-linked immunosorbent assay for igg antibodies to epstein-barr virus-associated early antigens and viral capsid antigen. | an enzyme-linked immunosorbent assay has been developed for the detection of igg antibodies to epstein-barr virus-associated early antigens and late antigens including the viral capsid antigen. the antibody titers of human sera determined in this way correlate well with those by indirect immunofluorescence. elisa was more sensitive than the if method. the assays described may be used for rapid and sensitive diagnosis of ebv-related diseases. in addition, the elisa will be useful for the determin ... | 1984 | 6323578 |
| modulation by the polyoxotungstate hpa-23 of epstein-barr virus early antigen expression in raji cells treated with iododeoxyuridine. | the polyoxotungstate hpa-23 was found to exert a differential effect on the induction of epstein-barr virus early antigen in raji cells induced with 5-iodo-2'-deoxyuridine (iudr). thus, treatment of raji cells concomitantly with iudr and hpa-23 inhibited early antigen expression, and the extent of inhibition was proportional to the duration of treatment with hpa-23. in contrast, pretreatment of raji cells with hpa-23 prior to induction with iudr stimulated early antigen expression in exponential ... | 1984 | 6323625 |
| interferon production by epstein-barr virus in human mononuclear leukocytes. | interferon (ifn) production following exposure to epstein-barr virus (ebv) was studied in primary human mononuclear leukocytes. when the leukocytes were exposed to ebv (strain b95-8 or p3hr-1) the ifn level reached a maximum 24 h after exposure to the virus, and a gradual decrease followed. a linear relationship was obtained between the input dose of ebv and the ifn titre. the ifn inducibility of leukocytes did not correlate with the ebv infectibility. ifn was also induced by u.v.-inactivated eb ... | 1984 | 6323626 |
| epstein-barr virus infections in pregnancy: a prospective study. | we studied 4063 pregnant women during 4108 gestations for evidence of infection with epstein-barr virus. forty-six (1.1%) were susceptible early in gestation, and only three showed seroconversion. two of the infants born to these three women were normal, one had tricuspid atresia. none of the infants had igm antibodies to ebv at birth, and their cord blood lymphocytes did not give rise spontaneously to ebv-carrying lymphoblastoid cell lines. thus we were unable to document intrauterine infection ... | 1984 | 6323662 |
| immunobiochemical characterization with monoclonal antibodies of epstein-barr virus-associated early antigens in chemically induced cells. | five monoclonal antibodies which are reactive to early antigens of epstein-barr virus have been produced by using somatic cell hybridization techniques. the specificity of the monoclonal antibodies to early antigens was demonstrated by indirect immunofluorescence, which showed that the antigens were localized to the nucleus of early antigen-induced raji cells. additional indirect immunofluorescence studies showed that like patient antisera to diffuse-staining early antigen, the monoclonal antibo ... | 1984 | 6323737 |
| cytomegalovirus and carcinogenesis. | 1984 | 6323806 | |
| presence of epstein-barr virus dna in carcinomas of the palatine tonsil. | the presence of epstein-barr virus genetic material was demonstrated in thin sections of biopsy specimens in 6 of 7 carcinomas of human palatine tonsil by the in situ hybridization test. the biopsy specimens from 4 tonsillar carcinomas exhibited a strong positive reaction; two biopsy specimens were weakly positive. two tumor-free biopsy specimens were negative. none of the thin sections reacted with 3h-labeled herpes simplex virus dna probe. | 1984 | 6323808 |
| anti-immunoglobulin inhibits dna synthesis in epstein-barr virus-transformed lymphoblastoid cell lines. | antibodies directed against immunoglobulin (anti-ig) were found to inhibit the spontaneous incorporation of [3h]thymidine by epstein-barr virus (ebv) transformed lymphoblastoid cell lines (lcl). the degree of inhibition was variable but could exceed 80% following a 24 hr exposure of cells to anti-ig. while a short pulse of antibody was sufficient to cause inhibition, maintainance of the effect required the continual presence of anti-ig in culture. maximal suppression by anti-ig of [3h]thymidine ... | 1984 | 6325331 |
| rheumatoid arthritis: evidence for a defect in t-cell function. | 1984 | 6325481 | |
| soluble factor requirements for the autostimulatory growth of b lymphoblasts immortalized by epstein-barr virus. | b lymphoblasts immortalized by the epstein-barr virus (ebv) exhibit autocrine growth stimulation--that is they release a soluble activity to which they respond by growth. a minimally supplemented serum-free medium conditioned by lymphoblastoid cells in their log phase of growth (lcl-cm) was found to contain autostimulatory activity allowing us to explore the mechanism of autocrine growth for these cell-types in defined conditions. below cell densities capable of supporting autonomous growth, con ... | 1984 | 6325575 |
| stress and the transformation of lymphocytes by epstein-barr virus. | although various stressors appear to influence herpesvirus infections, the underlying mechanisms have not been studied. a prospective design was used to examine the effects of examination stress and loneliness on the transformation of b lymphocytes in mixed cultures of t and b lymphocytes by epstein-barr virus (ebv). three blood samples were drawn from 42 ebv-seropositive medical students, with the baseline sample taken 1 month before their final examinations, the stress sample drawn on the firs ... | 1984 | 6325704 |
| [immunoblastic lymphadenopathy with abnormal high eb virus antibody]. | 1984 | 6325762 | |
| [present status of infectious mononucleosis and epstein barr virus]. | 1984 | 6325836 | |
| [epstein-barr anti-viral antibodies in childhood in relation to social status]. | 1984 | 6325859 | |
| immortalization of monkey epithelial cells by specific fragments of epstein-barr virus dna. | epstein-barr virus (ebv) is unique among the dna tumour viruses by virtue of its association with two human malignancies, burkitt's lymphoma and nasopharyngeal carcinoma (npc), the former a tumour of b lymphocytes and the latter encompassing low-differentiated epithelial cells of the nasopharynx. a viral gene product has not been definitively linked to these malignant diseases, although an ebv nuclear antigen(s) (ebna) seems to be ubiquitous in ebv-infected cells; indeed, the detection of ebna b ... | 1984 | 6325929 |
| long terminal repeats of human t-cell leukaemia virus ii genome determine target cell specificity. | human t-cell leukaemias and lymphomas associated with the human t-cell leukaemia viruses (htlv) are invariably neoplasms of cells with mature t-lymphocyte phenotype. epstein-barr virus-transformed b- lymphoycte lines which are productively infected with htlv may be isolated from patients with htlv malignancies, but no non-lymphoid tissues seem to be involved. here, to investigate the basis for this tissue specificity, we introduced type ii htlv (htlv-ii) into a variety of human cells by infectio ... | 1984 | 6325950 |
| epstein-barr virus and nasopharyngeal cancer. | 1984 | 6326009 | |
| epstein-barr virus and rheumatoid arthritis. | 1984 | 6326237 | |
| [the etiology of cancer: toward a unified theory]. | 1984 | 6326341 | |
| dna tumor viruses. | 1984 | 6326351 | |
| kaposi's sarcoma in homosexual men. a seroepidemiologic case-control study. | the cases of 20 male homosexuals with kaposi's sarcoma and the acquired immunodeficiency syndrome were compared with those of 40 age- and race-matched male homosexual controls. patients with kaposi's sarcoma had lower okt4/okt8 (t-helper/t-suppressor) ratios than controls, due to smaller numbers of okt4 cells. serum igg concentrations and antibody titers to cytomegalovirus in patients exceeded those in controls, but patients had lower antibody titers to epstein-barr virus. logistic regression an ... | 1984 | 6326631 |
| latent herpesviruses of humans. | the herpesviruses that infect humans characteristically establish a latent infection that may be reactivated later. the consequences of reactivation range from asymptomatic shedding to severe disseminated infection. varicella-zoster and herpes simplex viruses are both highly neurotropic, establishing nonreplicating infections in sensory ganglia. latent herpes simplex virus is known to reside in neurons, and the virus-cell interactions involved have been defined to an extent. cytomegalovirus and ... | 1984 | 6326635 |
| acyclovir and other chemotherapy for herpes group viral infections. | the recent profusion of antiviral research has resulted in significant advances toward prevention and treatment of herpes group virus infections. the most promising new agent is acyclovir, which is available in topical, intravenous, and oral formulations. results of clinical trials of acyclovir for prevention and treatment of herpes simplex, varicella-zoster virus, cytomegalovirus, and epstein-barr virus infections are discussed, and the potential problem of antiviral resistance considered. vida ... | 1984 | 6326661 |
| abnormal expansions of polyclonal large to small size granular lymphocytes: reactive or neoplastic process? | morphological, immunologic, and functional properties of peripheral blood cells from two patients with chronic proliferations of granular lymphocytes are described. cells from both patients showed a heterogeneous pattern from both a morphological and immunologic standpoint, indicating a polyclonal, rather than a monoclonal, expansion of these cells. in fact, both large and small-to-medium-sized granular lymphocytes were observed, and different percentages of positivity were found in the analysis ... | 1984 | 6326893 |
| evidence for a multistep pathogenesis of a myelodysplastic syndrome. | somatic cell genetic approaches utilizing the cellular mosaicism present in women heterozygous for glucose-6-phosphate dehydrogenase (g6pd) have provided information relevant to the pathogenesis of some neoplastic disorders. with these techniques, we studied a 61-year-old woman with a myelodysplastic syndrome. gdb/gda heterozygosity was demonstrated in skin and cultured t lymphocytes, which exhibited both a and b type g6pd. in contrast, erythrocytes, platelets, granulocytes, and marrow nucleated ... | 1984 | 6326894 |
| a strategy for the production of human monoclonal antibodies reactive with lung tumor cell lines. | epstein-barr virus (ebv)-immortalized cell lines were established from lymphocytes derived from peripheral blood, draining lymph nodes, bone marrow aspirates, tumors, and pericardial effusions from lung cancer patients. ten of these lines were cloned and screened against glutaraldehyde-fixed lung tumor cells for tumor-specific antibody production using an enzyme-linked immunosorbent assay. none of the 140 clones tested secreted specific antibody, suggesting that b-lymphocytes specific for tumor ... | 1984 | 6327024 |
| the eb virus genome in daudi burkitt's lymphoma cells has a deletion similar to that observed in a non-transforming strain (p3hr-1) of the virus. | epstein-barr virus (ebv) dna isolated from the frequently studied and unusual burkitt's lymphoma cell line, daudi, contains a 7.4-kb deletion, similar to (but larger than) that found in a non-transforming isolate of the virus, p3hr-1. a comparison of ebv sequence in daudi cells with that from a comparable region in a wild-type, transforming strain of the virus (b95-8) indicates that at least two of the previously identified rnas, a highly repetitive sequence, and other interesting coding or stru ... | 1984 | 6327290 |
| analysis of antigen uptake and presentation by epstein-barr virus-transformed human lymphoblastoid b cells. | epstein-barr virus-transformed human b cells (ebv-b cells), but not resting b cells or b cells activated by t cell-derived factors, have been shown to support the proliferation of tetanus toxoid (tt)-specific autologous t cell clones in response to tt antigen. the accessory cell function of ebv-b cells was compared to that of monocytes with regard to antigen uptake and processing. after an 18-h incubation period with 125i-labeled tt, the amount of radioactivity associated with the cells (approxi ... | 1984 | 6327319 |