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female sexual preferences toward conspecific and hybrid male mating calls in two species of polygynous deer, cervus elaphus and c. nippon.the behavioral processes at the basis of hybridization and introgression are understudied in terrestrial mammals. we use a unique model to test the role of sexual signals as a reproductive barrier to introgression by investigating behavioral responses to male sexual calls in estrous females of two naturally allopatric but reproductively compatible deer species, red deer and sika deer. previous studies demonstrated asymmetries in acoustic species discrimination between these species: most but not ...201727217596
dispersal ecology informs design of large-scale wildlife corridors.landscape connectivity describes how the movement of animals relates to landscape structure. the way in which movement among populations is affected by environmental conditions is important for predicting the effects of habitat fragmentation, and for defining conservation corridors. one approach has been to map resistance surfaces to characterize how environmental variables affect animal movement, and to use these surfaces to model connectivity. however, current connectivity modelling typically ...201727657496
glutathione in combination with trehalose has supplementary beneficial effects on cryopreserved red deer (cervus elaphus) sperm.in this study, we evaluated the effects of glutathione in combination with trehalose addition to semen extenders on the quality parameters of frozen-thawed red deer (cervus elaphus) spermatozoa.201727885744
low copper levels associated with low carcass weight in wild red deer ( cervus elaphus ) in norway.copper (cu) deficiency is associated with several disease syndromes, including poor growth, in farmed red deer ( cervus elaphus ), but little is known of the consequences of low cu levels in free-ranging populations. low hepatic cu levels have been documented in several wild red deer populations along the west coast of norway, with the lowest values found on the island of hitra. we studied the relationship between liver cu concentration and slaughter weight in 63 red deer calves and 69 yearlings ...201727788057
expression of metallothionein in the liver and kidneys of the red deer (cervus elaphus l.) from an industrial metal smelting area of poland.the metallothionein 1 (mt1) coding sequence of red deer was identified and compared to orthologous sequences from other mammals. over 90% identity was observed between red deer mt1 amino acid sequence and mt1 sequences of other ruminants. liver and kidney samples of red deer were collected from the industrial zinc smelting site of miasteczko slaskie and from the masuria lake district serving as a pollution-free control site. the concentrations of cadmium (cd), lead (pb), copper (cu) and zinc (zn ...201727918943
does cu supplementation affect the mechanical and structural properties and mineral content of red deer antler bone tissue?the main factors affecting the mechanical (and other) properties of bone, including antler, are the proportions of ash (especially ca and p) and collagen content. however, some trace minerals may also play more important roles than would be expected, given their low levels in bone and antler. one such trace mineral is cu. here, we studied the effects of cu supplementation on the mechanical and structural characteristics, and mineral content of antlers from yearling and adult (4 years of age) red ...201728069103
climate, female traits and population features as drivers of breeding timing in mediterranean red deer populations.understanding the factors that lead to variation in the timing of breeding in widespread species such as red deer (cervus elaphus l.) is crucial to predict possible responses of wild populations to different climate scenarios. here, we sought to further understand the causes of inter-annual variation in the reproduction timing of female deer in mediterranean environments. an integrative approach was used to identify the relative importance of individual, population and climate traits in the date ...201728054452
effect of sex-sorting and cryopreservation on the post-thaw sperm quality of iberian red deer spermatozoa.this study investigated the effect of sex-sorting and cryopreservation on post-thaw characteristics and fertility of red deer (cervus elaphus) sperm for the first time. semen was collected by electroejaculation from 10 mature stags during the breeding season, and each ejaculate split into four experimental groups: bulk sorted spermatozoa, sorted but not sexed (bss); sorted high purity x-spermatozoa (xss); sorted high purity y-spermatozoa (yss); and, control non-sorted spermatozoa (ns). following ...201728043354
estimating inbreeding rates in natural populations: addressing the problem of incomplete pedigrees.understanding and estimating inbreeding is essential for managing threatened and endangered wildlife populations. however, determination of inbreeding rates in natural populations is confounded by incomplete parentage information. we present an approach for quantifying inbreeding rates for populations with incomplete parentage information. the approach exploits knowledge of pedigree configurations that lead to inbreeding coefficients of f = 0.25 and f = 0.125, allowing for quantification of pr(i ...201728398480
a mortality event in elk ( cervus elaphus nelsoni) calves associated with malnutrition, pasteurellosis, and deer adenovirus in colorado, usa.this report describes clinical, necropsy, and ancillary diagnostic findings for a mortality event in rocky mountain elk ( cervus elaphus nelsoni) calves attributed to malnutrition, pasteurellosis, and an alimentary presentation of adenovirus hemorrhagic disease.201728328349
can red deer antlers be used as an indicator of environmental and edible tissues' trace element contamination?venison is an attractive product for consumers concerned with healthy lifestyle; however, it can contain high levels of toxic elements, and therefore, it is a possible source of hazardous contaminants in human diet. antlers are suitable bioindicators of environmental metal contamination, and herein, we assessed the ability of trace element levels in antlers to indicate levels in edible soft tissues. we determined the concentrations of lead (pb), cadmium (cd), mercury (hg), arsenic (as), copper ( ...201728324255
optimization of protocols for iberian red deer (cervus elaphus hispanicus) sperm handling before sex sorting by flow cytometry.currently, sperm reproductive biotechnologies such as sex sorting and cryopreservation are undoubtedly valuable tools for improving the economic and biological efficiency of red deer production systems. in this context, and because of the particular characteristics of this species (extensive exploitation typically far from laboratory facilities), a key goal is to optimize the design of an adequate handling protocol of sperm samples before samples are subjected to sex sorting and cryopreservation ...201728237327
effects of attitudes and demography on public support for endangered species conservation.it is critical for managers to understand how attitudes and demography affect public's preferences for species protection for designing successful conservation projects. 1080 adults in greece were asked to rate pictures of 12 endangered species on aesthetic and negativistic attitudes, and intention to support their conservation. factor analysis identified a group of animals for which respondents indicated high levels of support for their conservation (red deer, loggerhead sea turtle, brown bear, ...201728376425
control of bovine tuberculosis in a farmed red deer herd in england.this report describes how mycobacterium bovis infection was controlled and eventually eradicated in a farmed red deer herd in the north of england, following sustained tuberculin skin testing supplemented with serological (antibody) tests over a period of approximately two years. by taking advantage of the anamnestic antibody response produced by the skin test to detect skin test-negative, antibody-positive infected individuals, a total of 35 additional animals were identified, including 2 with ...201728100768
detection of mycobacterium avium subsp. paratuberculosis in kidney samples of red deer (cervus elaphus) in portugal: evaluation of different methods.paratuberculosis or johne's disease, caused by mycobacterium avium subsp. paratuberculosis (map), is a chronic granulomatous enteritis affecting both domestic and wild ruminants. the present work is part of a wider set of studies designed to assess the prevalence of paratuberculosis in free ranging red deer (cervus elaphus). with that purpose, 877 free-ranging red deer legally hunted in the centre-eastern portugal were submitted to necropsy and sampled for molecular methods, microbiology and his ...201728132959
hepatitis e virus in wild boars and spillover infection in red and roe deer, germany, 2013-2015.to determine animal hepatitis e virus (hev) reservoirs, we analyzed serologic and molecular markers of hev infection among wild animals in germany. we detected hev genotype 3 strains in inner organs and muscle tissues of a high percentage of wild boars and a lower percentage of deer, indicating a risk for foodborne infection of humans.201727983488
presence of hepatitis e virus in a red deer (cervus elaphus) population in central italy.hepatitis e is an acute human disease caused by the hepatitis e virus (hev). in addition to humans, hev has been detected in several animal species and is recognized as a zoonotic pathogen. pigs, wild boar and deer can be reservoir. in this study, we evaluated hev prevalence in a free-living red deer (cervus elaphus) population in central italy by detecting virus-specific antibodies and rna in sera. a total of 35 of 251 red deer sera were positive for anti-hev igg. hev rna was detected in 10 of ...201725892400
foot-and-mouth disease in red deer - experimental infection and test methods performance.the aim of this study was to evaluate a number of foot-and-mouth disease (fmd) test methods for use in red deer. ten animals were intranasally inoculated with the fmd virus (fmdv) o ukg 11/2001, monitored for clinical signs, and samples taken regularly (blood, serum, oral swabs, nasal swabs, probang samples and lesion swabs, if present) over a 4-week period. only one animal, deer 1103, developed clinical signs (lesions under the tongue and at the coronary band of the right hind hoof). it tested ...201725907028
wide exposure to coxiella burnetii in ruminant and feline species living in a natural environment: zoonoses in a human-livestock-wildlife interface.assessment of the role of wild and domestic hosts as potential reservoirs of misdiagnosed zoonoses, such as q fever by coxiella burnetii, is an important public health issue today both for wildlife conservation and management of disease in human-livestock-wildlife interface. this study used elisa, an indirect antibody, to research (2003-2013) c. burnetii infection in seven free-living wild and domestic ruminant species and in european wildcats (felis silvestris). the animals studied were 0 europ ...201727776577
antimicrobial susceptibility and genetic characterization of escherichia coli recovered from frozen game meat.the increasing number of antimicrobial resistant enterobacteriaceae both in veterinary and human medicine, the dissemination of these bacteria in several environments and their possible repercussions on human health is causing concern. game meat is usually seen as free of antimicrobial resistant bacteria. the objective of this study was to evaluate the current antimicrobial susceptibility status in generic escherichia coli isolated from packed frozen game meat from a game handling establishment ...201728040165
absence of antibodies specific to besnoitia spp. in european sheep and goats from areas in spain where bovine besnoitiosis is endemic.besnoitia besnoiti and b. caprae, which infect bovids (cattle and antelopes) and goats, respectively, are responsible for besnoitiosis, a chronic and debilitating disease. bovine besnoitiosis is considered to be a reemerging disease in central and western europe. in addition, infection by besnoitia spp. has been reported in reindeer from sweden and finland. recently, the parasite was also detected in roe deer and red deer from spain, where an interconnection between the domestic and sylvatic cyc ...201727815735
brucellosis transmission between wildlife and livestock in the greater yellowstone ecosystem: inferences from dna genotyping.the wildlife of the greater yellowstone ecosystem carries brucellosis, which was first introduced to the area by cattle in the 19th century. brucellosis transmission between wildlife and livestock has been difficult to study due to challenges in culturing the causative agent, brucella abortus . we examined b. abortus transmission between american bison ( bison bison ), rocky mountain elk ( cervus elaphus nelsoni), and cattle ( bos taurus ) using variable number tandem repeat (vntr) markers on dn ...201728118557
wildlife interactions on baited places and waterholes in a french area infected by bovine tuberculosis.interactions among wildlife species are major drivers for the transmission of multi-host pathogens, such as mycobacterium bovis, which also affect livestock. although france is officially free from bovine tuberculosis (btb), some areas are still harboring infection in cattle and wildlife. we aimed at characterizing the visits of susceptible wild species (badger, red deer, and wild boar) at baited places and waterholes, considered as possible hotspots for contacts. we described the visits in term ...201728138439
molecular analysis of anaplasma phagocytophilum and babesia divergens in red deer (cervus elaphus) in western austria.wild ungulates may act as reservoirs of various vector borne pathogens that can infect humans and domestic animals. in the present study, blood samples from 196 red deer (cervus elaphus) from western austria (vorarlberg, tyrol and salzburg) were collected on filter paper and tested for anaplasmataceae, piroplasmida, rickettsia and filarioid helminths using molecular tools. babesia divergens was detected in ten (5.1%) and anaplasma phagocytophilum in three (1.5%) of the 196 samples. filarioid hel ...201727417532
rapid and robust authentication of deer antler velvet product by fast pcr-rflp analysis.deer antler velvet is widely used as a vitalizing, tonifying, haemopoietic and strengthening agent for debilitated persons in east asia. to develop a rapid and sensitive method for the identification of the biological source or origin in antler velvet products, a molecular approach was applied using pcr-restriction fragment length polymorphism analysis. the cytochrome b gene sequences of nine cervidae species were analyzed, and a dde i restriction endonuclease recognition site was found only in ...201828071968
development and validation of a fallow deer (dama dama)-specific taqman real-time pcr assay for the detection of food adulteration.the aim of the present study was to develop a real-time pcr assay for the identification and quantification of fallow deer (dama dama) in food to detect food adulteration. despite high sequence homology among different deer species, a fallow deer-specific primer/probe system targeting a fragment of the nuclear mc1-r gene was designed. this primer/probe system did not amplify dna from 19 other animals and 50 edible plant species. moderate cross-reactivity was observed for sika deer, red deer, roe ...201829146373
first detection and genetic characterisation of enterocytozoon bieneusi in wild deer in melbourne's water catchments in australia.enterocytozoon bieneusi is reported to be a common microsporidian of humans and animals in various countries. however, e. bieneusi has yet to be recorded in animals in australia. here, we undertook the first molecular epidemiological investigation of e. bieneusi in three species of deer (cervus elaphus, dama dama and rusa unicolor) that live in the catchment areas that supply the city of melbourne with drinking water.201829295716
genetic diversity, genetic structure and diet of ancient and contemporary red deer (cervus elaphus l.) from north-eastern france.in north-eastern france, red deer (cervus elaphus l.) populations were rebuilt from a few hundred individuals, which have subsisted in remote valleys of the vosges mountains, and to a lesser extent from individuals escaped from private enclosures; at present times, this species occupies large areas, mainly in the vosges mountains. in this study, we examined the population dynamics of red deer in the vosges mountains using ancient and contemporary mitochondrial dna (mtdna) from 140 samples (23 an ...201829304165
social rank affects the haematologic profile in red deer hinds.we studied the effects of social rank on the haematologic profile in a herd of 24 female iberian red deer hinds. social rank hierarchy was determined and blood samples were taken and analysed. after adjusting for age and body mass, dominance ranking showed a significant negative effect (ie, lower values in dominant hinds) on white blood cell (wbc) count, haemoglobin and haematocrit. our results are similar to those reported for stressed individuals due to physical immobilisation, but do not supp ...201829374098
age-dependent changes in essential elements and oxidative stress biomarkers in blood of red deer and vulnerability to nutritional deficiencies.changes in the concentration of circulating essential elements in animals over life may be indicative of periods of vulnerability to deficiencies and associated diseases. here we studied age-related variations in essential elements (se, cu, zn and mn) and some selected oxidative stress biomarkers (gpx, sod, vitamin a and vitamin e) in blood of an iberian red deer (cervus elaphus hispanicus) population living in semicaptive conditions. animals during their first year of life showed to be especial ...201829353781
toxoplasma gondii in sympatric domestic and wild ungulates in the mediterranean ecosystem.toxoplasma gondii is a zoonotic protozoan of worldwide distribution. the present study provides information on risk factors affecting t. gondii infection in domestic and free-ranging wild ungulates sharing habitats in mediterranean ecosystems in spain. serum samples from 482 extensively reared domestic ruminants and 2351 wild ungulates were tested for t. gondii antibodies using the modified agglutination test (mat, cut-off 1:25). toxoplasma gondii seroprevalence was 41.2% of 194 sheep, 18.6% of ...201829344801
epizoochorous dispersal by ungulates depends on fur, grooming and social interactions.the transport phase of the animal-mediated plant dispersal process is critical to dispersal effectiveness as it determines the spatial distribution of the diaspores released and their chance for further recruitment. assessing this specific phase of the dispersal process generally requires combining diaspore retention times with the associated distances covered. here, we specifically tested the effect of grooming behavior, interindividual contacts and ungulate fur on diaspore retention times and ...201829435234
traditional cattle vs. introduced deer management in chaco serrano woodlands (argentina): analysis of environmental sustainability at increasing densities.wild ungulate populations have increased and expanded considerably in many regions, including austral woodlands and forests where deer (cervus elaphus) have been introduced as an alternative management to traditional cattle grazing. in this study, we compared traditional cattle with introduced deer management at increasing deer densities in the "chaco serrano" woodlands of argentina to assess their ecological sustainability. we used three ecological indicators (abundance of tree regeneration, wo ...201829132087
oocyte holding in the iberian red deer (cervus elaphus hispanicus): effect of initial oocyte quality and epidermal growth factor addition on in vitro maturation.current in vitro embryo production protocols in the iberian red deer (cervus elaphus hispanicus) need to be optimized; oocyte harvesting in situ followed by overnight holding could reduce the human effort and shipping costs. in our work, post-mortem ovaries were retrieved, and the oocytes were harvested and allocated to g1 group (good quality) or g2 + g3 group (low quality). the oocytes were separately subjected to immediate in vitro maturation (ivm) or held overnight in a holding medium compose ...201829110393
pharmacokinetics of articaine hydrochloride and its metabolite articainic acid after subcutaneous administration in red deer (cervus elaphus).to develop and validate a simple and sensitive method using liquid chromatography-mass spectrometry (lc-ms) for quantification of articaine, and its major metabolite articainic acid, in plasma of red deer (cervus elaphus), and to investigate the pharmacokinetics of articaine hydrochloride and articainic acid in red deer following s/c administration of articaine hydrochloride as a complete ring block around the antler pedicle.201829020878
supplemental feeding alters migration of a temperate ungulate.conservation of migration requires information on behavior and environmental determinants. the spatial distribution of forage resources, which migration exploits, often are altered and may have subtle, unintended consequences. supplemental feeding is a common management practice, particularly for ungulates in north america and europe, and carryover effects on behavior of this anthropogenic manipulation of forage are expected in theory, but have received limited empirical evaluation, particularly ...201829210236
pathologic and biochemical characterization of prpscfrom elk with prnp polymorphisms at codon 132 after experimental infection with the chronic wasting disease agent.the rocky mountain elk (cervus elaphus nelsoni) prion protein gene (prnp) is polymorphic at codon 132, with leucine (l132) and methionine (m132) allelic variants present in the population. in elk experimentally inoculated with the chronic wasting disease (cwd) agent, different incubation periods are associated with prnp genotype: ll132 elk survive the longest, lm132 elk are intermediate, and mm132 elk the shortest. the purpose of this study was to investigate potential mechanisms underlying vari ...201829523205
very low influenza a virus prevalence in cervids in german national parks.influenza a viruses are one of the most important and most studied pathogens in humans and domestic animals but little is known about viral prevalence in non-avian wildlife. serum samples from three free-ranging cervid species (red [ cervus elaphus], fallow [ dama dama] , and roe deer [ capreolus capreolus]) were collected from six german national parks between 2000 and 2002. the serum was tested for the presence of influenza a antibodies using a commercial competitive enzyme-linked immunosorben ...201829517428
serological surveillance for tahyna virus (california encephalitis orthobunyavirus, peribunyaviridae) neutralizing antibodies in wild ungulates in austria, hungary and romania.a serosurvey for tahyna virus (tahv), a mosquito-borne california encephalitis orthobunyavirus (peribunyaviridae) endemic to europe, was performed to estimate the activity of tahv on a broad geographic scale. sera from wild boar (sus scrofa), roe deer (capreolus capreolus) and red deer (cervus elaphus) were collected from austria, hungary and romania. samples were tested for neutralizing antibodies against tahv using a virus microneutralization assay. the results demonstrate that tahv transmissi ...201829516663
a teat papillomatosis case in a damascus goat (shami goat) in hatay province, turkey: a new putative papillomavirus?papillomaviruses (pvs) are epitheliotropic viruses that cause benign proliferative lesions in the skin (warts or papillomas) and mucous membranes of their natural hosts. recently, new pvs have been found in many animal species. the most common current approach for identifying novel pv types is based on pcr, using various consensus or degenerated primer (broad-range primers), designed on the basis of the multiple alignment of nucleotide or amino acid sequences of a large number of different human ...201829502149
prevalence and phylogenetic analysis of hepatitis e virus in pigs, wild boars, roe deer, red deer and moose in lithuania.hepatitis e virus (hev) is one of the major causes of acute viral hepatitis worldwide. in europe, food-borne zoonotic transmission of hev genotype 3 has been associated with domestic pigs and wild boar. controversial data are available on the circulation of the virus in animals that are used for human consumption, and to date, no gold standard has yet been defined for the diagnosis of hev-associated hepatitis. to investigate the current hev infection status in lithuanian pigs and wild ungulates, ...201829471843
spectrum of antibody profiles in tuberculous elephants, cervids, and cattle.using multi-antigen print immunoassay and dpp®vettb assay approved in the united states for testing captive cervids and elephants, we analyzed antibody recognition of mpb83 and cfp10/esat-6 antigens in asian elephants (elephas maximus) infected with mycobacterium tuberculosis and in white-tailed deer (odocoileus virginianus), fallow deer (dama dama), elk (cervus elaphus), and cattle (bos taurus) infected with mycobacterium bovis. serum igg reactivity to mpb83 was found in the vast majority of tu ...201829408038
the red deer cervus elaphus genome cerela1.0: sequencing, annotating, genes, and chromosomes.we present here the de novo genome assembly cerela1.0 for the red deer, cervus elaphus, an emblematic member of the natural megafauna of the northern hemisphere. humans spread the species in the south. today, the red deer is also a farm-bred animal and is becoming a model animal in biomedical and population studies. stag dna was sequenced at 74× coverage by illumina technology. the allpaths-lg assembly of the reads resulted in 34.7 × 103scaffolds, 26.1 × 103of which were utilized in cer.ela1.0. ...201829294181
molecular identification of sarcocystis halieti n. sp., sarcocystis lari and sarcocystis truncata in the intestine of a white-tailed sea eagle (haliaeetus albicilla) in norway.an emaciated white-tailed sea eagle (haliaeetus albicilla) from western norway was found and nursed briefly before it died. the necropsy revealed that the principal cause of death was an inflammation and occlusion of the bile ducts. a secondary finding was the presence in the intestinal mucosa of numerous sporulated sarcocystis oocysts measuring 21.8-22.8 × 16.0-17.0 μm. the aim of this study was to identify these oocysts to species level using molecular methods. genomic dna was extracted from 1 ...201829270360
[structural–functional condition of the thyroid gland of red deer at different stages of postnatal ontogenesis].the histological structure and morphometric and some histochemical indices of thyroid gland ofmaral as a subspecies of red deer at the postnatal stage of ontogenesis were studied. it was established that thegland was structurally formed by the time of the birth. c-cells are detected at 6 months of age. with age, theratio of follicles of different sizes changed: smaller forms predominate in newborns and those 6 months old,medium and large predominate in adults and in old deer. histological, morph ...201830272886
tetraplex real-time pcr assay for the simultaneous identification and quantification of roe deer, red deer, fallow deer and sika deer for deer meat authentication.analytical methods are needed for the identification and quantification of meat species to detect food adulteration. since game meat is more expensive than meat from domesticated animal species, it is a potential target for adulteration. we present a tetraplex real-time pcr assay that allows the simultaneous determination of the content of roe deer, red deer, fallow deer and sika deer. the tetraplex assay showed only moderate cross-reactivity with closely related species. after optimization the ...201830100464
evidence of two genetically different lymphotropic herpesviruses present among red deer, sambar, and milu herds in china.herpesvirus infections in cervidae are a serious threat affecting some deer species worldwide. in our attempt to identify malignant catarrhal fever-associated herpesviruses in deer herds, ten gammaherpesviral dna fragments were identified in five species of deer in herds in china by using a pan-herpesvirus polymerase chain reaction assay targeting viral dna polymerase. notably, in sambar (rusa unicolor), a novel gamma-2 herpesvirus was identified that showed a close relationship with fallow deer ...201830041287
susceptibility of human prion protein to conversion by chronic wasting disease prions.chronic wasting disease (cwd) is a contagious and fatal neurodegenerative disease and a serious animal health issue for deer and elk in north america. the identification of the first cases of cwd among free-ranging reindeer and moose in europe brings back into focus the unresolved issue of whether cwd can be zoonotic like bovine spongiform encephalopathy. we used a cell-free seeded protein misfolding assay to determine whether cwd prions from elk, white-tailed deer, and reindeer in north america ...201830014840
gas and short-chain fatty acid production from feeds commonly fed to red deer (cervus elaphus l.) and incubated with rumen inoculum from red deer and sheep.efficient red deer supplementary feeding depends on estimations of the nutritive value of offered feeds, frequently estimated with the use of equations derived from domestic ruminants. the aim of this study was to compare the 24-hour in vitro true dry matter degradability (ivtd24 ), in vitro gas production (gp) kinetic parameters, gp in 24 hr of incubation (gas24 ) and short-chain fatty acid (scfa) and microbial biomass (mbs) produced after 24-hour incubation of feeds in inoculum prepared from s ...201829978922
correction: genetic diversity, genetic structure and diet of ancient and contemporary red deer (cervus elaphus l.) from north-eastern france.[this corrects the article doi: 10.1371/journal.pone.0189278.].201829940045
prenatal histomorphological development of the rumen in dama dama.this work studies the morphological changes taking place in the dama dama rumen during prenatal development using histomorphometrics, surface microstructure and immunohistochemistry analysis as well as carrying out a comparative analysis of this species with other wild (red deer) and domestic-type ruminants. a total of 25 fallow deer embryos and fetuses were used, from the first stage of prenatal life until birth. the appearance of the rumen from the primitive gastric tube was observed at 51 day ...201829893403
cinnamtannin b-1, a novel antioxidant for sperm in red deer.cinnamtannin b-1 (cnb-1) is a naturally occurring trimeric a-type proanthocyanidin contained in several plants such as cinnamon (cinnamomum zeylanicum). it is considered to be a potent antioxidant. the protective effect of cnb-1 against oxidative stress was assessed in red deer epididymal sperm incubated at 37 °c. cryopreserved sperm from six stags were thawed, pooled and extended to 400 × 106 sperm/ml in bgm (bovine gamete medium). after being aliquoted, the samples were supplemented with diffe ...201829776697
common drivers of seasonal movements on the migration - residency behavior continuum in a large herbivore.this study aimed to (1) identify the scale of environmental drivers of seasonal movements on the migration - residency behavior continuum in a large herbivore species and to (2) test the hypothesis that the same environmental drivers and spatio-temporal scaling should influence spatial processes in both migrants (long distance migration) and residents (short distance range shifts). we performed a comparative analysis of the influence of plant phenology and snow cover duration on seasonal movemen ...201829769562
sika deer (cervus nippon)-specific real-time pcr method to detect fraudulent labelling of meat and meat products.since game meat is more valuable and expensive than meat from domesticated animal species it is a potential target for adulteration. analytical methods must allow the identification and quantification of meat species to be applicable for the detection of fraudulent labelling. we developed a real-time pcr assay for the authentication of sika deer (cervus nippon) and products thereof. the primer/probe system amplifies a 71 bp fragment of the kappa-casein precursor gene. since the target sequence c ...201829739996
genome sequences of two novel papillomaviruses isolated from healthy skin of pudu puda and cervus elaphus deer.we report the complete genome sequences of pudu puda papillomavirus1 (ppudpv1) and cervus elaphus papillomavirus2 (celapv2), isolated from healthy skin hair follicles of a southern pudu and a red deer, respectively. ppudpv1 is basal to the dyokappapvs, whereas celapv2 is basal to the xipvs (beta-xipv crown group).201829724829
long-term occurrence of trichuris species in wild ruminants in the czech republic.the aim of this study was to identify trichuris species in wild ruminants from 32 localities in the czech republic using morphological and molecular methods (its1-5.8s rna-its2 region polymorphisms). trichurids were obtained from 176 wild ruminants (roe deer, sika deer, red deer, fallow deer and mouflons) that were culled between 2009 and 2017. trichuris discolor is the predominant trichurid of all of the above-mentioned wild ruminants, whereas trichuris ovis was identified less frequently in ro ...201829721657
a novel application of an anthelmintic mixture for use against gastrointestinal parasites of red deer (cervus elaphus).a mixture of proprietary anthelmintics delivering 0.5 mg/kg moxidectin, 9.06 mg/kg oxfendazole, 15 mg/kg levamisole, and 0.08 mg/kg selenium on bodyweight basis per os to red deer is investigated. on a deer farm with a history of parasite problems, six weaner red deer were treated orally with a 50/50 mixture of exodus pour-on and oxfen c plus (ex/ox) at a dose rate of 1 ml/5 kg bodyweight. six herd mates were untreated. eleven days later abomasal worm counts for the untreated deer revealed an ar ...201829692928
pregnancy and mid-term abortion rates in farmed red deer in new zealand.this paper describes pregnancy and mid-term abortion rate data from an investigation of sub-optimal reproductive performance in new zealand farmed red deer. for 2 years, 87 yearling (yl) and 71 mixed-age adult (ma) herds from 85 farms were observed with 15 yl and seven ma herds observed both years. hinds were pregnancy diagnosed using ultrasonography in the late autumn-early winter (pd1) and again late winter-early spring (pd2) and mid-term daily abortion rates (dar) were calculated. overall, 85 ...201829685706
draft whole-genome sequences of three diarrheagenic escherichia coli strains isolated from farmed deer in new zealand.escherichia coli bacteria commonly colonize the gastrointestinal tracts of farmed ruminants. cattle are a well-recognized reservoir of zoonotic e. coli; we report here, however, the draft genome sequences of three diarrheagenic e. coli strains isolated from farmed red deer (cervus elaphus) in the manawatu region of new zealand.201829674551
transmission of helminths between species of ruminants in austria appears more likely to occur than generally assumed.helminth infections of the gastrointestinal tract and lungs can lead to devastating economical losses to the pastoral based animal production. farm animals can suffer from malnutrition, tissue damage, and blood loss resulting in impaired production traits and reproduction parameters. in austria, pastures grazed by sheep, goats, and cattle overlap with the habitats of several species of wild cervids (roe deer, red deer, sika deer, and fallow deer) and bovids (mouflon, chamois, and ibex), and tran ...201829662884
declining home range area predicts reduced late-life survival in two wild ungulate populations.demographic senescence is increasingly recognised as an important force shaping the dynamics of wild vertebrate populations. however, our understanding of the processes that underpin these declines in survival and fertility in old age remains limited. evidence for age-related changes in foraging behaviour and habitat use is emerging from wild vertebrate studies, but the extent to which these are driven by within-individual changes, and the consequences for fitness, remain unclear. using longitud ...201829656580
evaluation of factors affecting the efficacy of treatment against fascioloides magna in wild red deer population.deer fascioloidosis is a serious and potentially fatal disease caused by the non-native trematode fascioloides magna. infections of red deer with f. magna in croatia have been reported for the first time in 2000 in the baranja region. subsequently, the disease spread throughout the eastern parts of the country, involving all 3 deer species (red, roe, and fallow) and mouflons. within the disease control programme (dcp), livers from all shot deer were thoroughly analysed and all detected trematode ...201829631313
enamel apatite crystallinity significantly contributes to mammalian dental adaptations.the monophyodont molar teeth, prismatic enamel and the complexity of enamel microarchitecture are regarded as essential dental apomorphies of mammals. as prominent background factors of feeding efficiency and individual longevity these characters are crucial components of mammalian adaptive dynamics. little is known, however, to which degree these adaptations are influenced by the crystallographic properties of elementary hydroxyapatite crystallites, the only inorganic component of enamel. in a ...201829615748
mycobacterium aviumsubsp. paratuberculosis isolated from wild red deer (cervus elaphus) in northern italy.paratuberculosis (or johne's disease) is an infectious disease which affects mainly ruminants and it is caused by mycobacterium avium subsp. paratuberculosis (map). during a culling program (years 2011-2015) aimed at controlling the red deer (cervus elaphus) population in stelvio national park (italian alps), where paratuberculosis was already described in this species, 382 tissue samples from the lombardy region and 102 fecal specimens from the autonomous province of bolzano were analyzed by pc ...201829615250
identification of first-stage dorsal-spined lungworm larvae of tunisian barbary red deer: first report of varestrongylus sagittatus and elaphostrongylus cervi in africa.barbary red deer (cervus elaphus barbarus) is a protected rare subspecies of red deer. the study of its protostrongylidae fauna based only on sporadic necropsy of naturally dead animals is difficult. therefore diagnosis of lungworms rely mainly on the identification of the first stage larvae (l1). the l1 of the different species are not readily diagnosed on morphological basis since much variation is recorded within and among dorsal-spined larvae belonging to various species. the aim of this stu ...201829581029
lower dietary concentrate level increases bacterial diversity in the rumen of cervus elaphus yarkandensis.the ruminal microbiota plays major roles in feed digestion. the composition and fermentation of the bacterial communities in 3 important ruminant species have been studied previously. here, we extended this research to the effect of concentrate-to-forage ratios on ruminal bacterial communities in tarim red deer (cervus elaphus yarkandensis). different concentrate-to-forage ratios (2:8, 3:7, 4:6, and 5:5) were fed to tarim red deer for 20 days. ruminal bacterial communities were elucidated by 16s ...201829562140
carcass and meat characteristics from farm-raised and wild fallow deer (dama dama) and red deer (cervus elaphus): a review.deer species are utilised for food, hunting and other products throughout the world. consumers are typically exposed to venison derived predominantly from both farm-raised or wild fallow (dama dama) and red deer (cervus elaphus). the production of venison under farm conditions, compared to the meat of deer hunted in the wild, allows for a regular supply of a consistently good meat. it is lean, tasty, and rich in proteins and minerals, with a low content of fat and cholesterol. overall, the world ...201829558697
influence of insemination time on the fertility of sex sorted frozen-thawed y-sperm in red deer.the aim of this study was to assess the effect of insemination timing on pregnancy rates in red deer (cervus elaphus) when using sex-sorted sperm samples. semen was collected by electroejaculation from 8 mature stags and processed to obtain: conventional samples, following standard freezing procedures for commercial purposes; control sorted samples, diluted and handled as per sorted samples but without being submitted to the sorter passage; and y sex sorted (yss) samples. hinds were synchronized ...201829547733
seasonality of helminth infection in wild red deer varies between individuals and between parasite taxa.parasitism in wild mammals can vary according to myriad intrinsic and extrinsic factors, many of which vary seasonally. however, seasonal variation in parasitism is rarely studied using repeated samples from known individuals. here we used a wild population of individually recognized red deer (cervus elaphus) on the isle of rum to quantify seasonality and intrinsic factors affecting gastrointestinal helminth parasitism over the course of a year. we collected 1020 non-invasive faecal samples from ...201829519265
effects of mn supplementation in late-gestating and lactating red deer (cervus elaphus hispanicus) on milk production, milk composition, and calf growth.this study describes the effects of mn supplementation of 20 late-gestating and lactating iberian red deer (cervus elaphus hispanicus) females (hinds) fed a balanced diet on milk production and milk composition over the lactation period. body weight of their calves at birth and at weaning was also evaluated. in addition, the effect of lactation stage was studied. for these purposes, 2 groups of hinds, one composed by 12 individuals (experimental) and the other by 8 individuals (control) were com ...201829518225
preventive effects of velvet antler (cervus elaphus) against lipopolysaccharide-induced acute lung injury in mice by inhibiting mapk/nf-κb activation and inducing ampk/nrf2 pathways.velvet antler (cervus elaphus) is a typical traditional animal medicine. it is considered to have various pharmacological effects including stimulation of the immune system, increase in the physical strength, and enhancement of sexual function. this paper aims to investigate the aqueous extract of velvet antler (ava) in the mouse models of lps-induced ali. inhibition of no, tnf-α, il-1β, il-6, and il-10 productions contributes to the attenuation of lps-induced lung inflammation by ava. a 5-day p ...201829483931
lack of association between leptospira spp. serovars hardjobovis and pomona and pregnancy and mid-term abortion in new zealand farmed red deer.this paper investigates leptospira borgpeterseni serovar hardjobovis and l. interrogans serovar pomona as potential causes of sub-optimum pregnancy rates and mid-term abortion in farmed red deer. rising two-year-old (r2, n = 22,130) and mixed-age (ma, n = 36,223) hinds from 87 and 71 herds, respectively, throughout new zealand were ultrasound scanned early in gestation (scan-1) and a sub-sample re-scanned (scan-2) 55-89 days later and mid-term daily abortion rate calculated. a sub-sample of sera ...201829426411
estimating abundance of an open population with an n-mixture model using auxiliary data on animal movements.accurate assessment of abundance forms a central challenge in population ecology and wildlife management. many statistical techniques have been developed to estimate population sizes because populations change over time and space and to correct for the bias resulting from animals that are present in a study area but not observed. the mobility of individuals makes it difficult to design sampling procedures that account for movement into and out of areas with fixed jurisdictional boundaries. aeria ...201829405475
scientific opinion on chronic wasting disease (ii).the european commission asked efsa for a scientific opinion on chronic wasting disease in two parts. part one, on surveillance, animal health risk-based measures and public health risks, was published in january 2017. this opinion (part two) addresses the remaining terms of reference, namely, 'are the conclusions and recommendations in the efsa opinion of june 2004 on diagnostic methods for chronic wasting disease still valid? if not, an update should be provided', and 'update the conclusions of ...201832625679
an assessment of the use of cox1 and cox3 mitochondrial genetic markers for the identification of dictyocaulus spp. (nematoda: trichostrongyloidea) in wild ruminants.lungworms of the genus dictyocaulus railliet and henry, 1907 (nematoda: trichostrongyloidea) are the causative agents of parasitic bronchitis (dictyocaulosis, husk) of various ungulate hosts, including domestic and wild ruminants. correct diagnosis of lungworm species and a better understanding of the transmission patterns of dictyocaulus spp. are crucial in minimising the risk of its cross transmission between wildlife and livestock, and for the control of dictyocaulosis. the study was conducte ...201829736729
low sequence diversity of the prion protein gene (prnp) in wild deer and goat species from spain.the first european cases of chronic wasting disease (cwd) in free-ranging reindeer and wild elk were confirmed in norway in 2016 highlighting the urgent need to understand transmissible spongiform encephalopathies (tses) in the context of european deer species and the many individual populations throughout the european continent. the genetics of the prion protein gene (prnp) are crucial in determining the relative susceptibility to tses. to establish prnp gene sequence diversity for free-ranging ...201829631620
diverse tick-borne microorganisms identified in free-living ungulates in slovakia.free-living ungulates are hosts of ixodid ticks and reservoirs of tick-borne microorganisms in central europe and many regions around the world. tissue samples and engorged ticks were obtained from roe deer, red deer, fallow deer, mouflon, and wild boar hunted in deciduous forests of south-western slovakia. dna isolated from these samples was screened for the presence of tick-borne microorganisms by pcr-based methods.201830176908
game meat consumption by hunters and their relatives: a probabilistic approach.this study aimed to estimate the consumption of meat and products derived from hunting by the consumer population and, specifically, by hunters and their relatives. for this purpose, a survey was conducted on the frequency of consuming meat from the four most representative game species in spain, two of big game, wild boar (sus scrofa) and red deer (cervus elaphus), and two of small game, rabbit (oryctolagus cuniculus) and red partridge (alectoris rufa), as well as of processed meat products (sa ...201829912678
tick- and fly-borne bacteria in ungulates: the prevalence of anaplasma phagocytophilum, haemoplasmas and rickettsiae in water buffalo and deer species in central europe, hungary.hunting constitutes an important industry in europe. however, data on the prevalence of vector-borne bacteria in large game animal species are lacking from several countries. blood or spleen samples (239 and 270, respectively) were taken from red, fallow and roe deer, as well as from water buffaloes, mouflons and wild boars in hungary, followed by dna extraction and molecular analyses for anaplasma phagocytophilum, haemoplasmas and rickettsiae.201829554900
perceptions and acceptability of some stakeholders about the bovine tuberculosis surveillance system for wildlife (sylvatub) in france.bovine tuberculosis (btb) is a common disease of cattle and wildlife, with economic repercussions and implications for animal and human health. the surveillance of btb in wildlife is particularly important, to shed light on the epidemiological role of wild species and for the adaptation of control measures. in france, a btb surveillance system for free-ranging wildlife, the sylvatub system, was launched in 2011 on wild boars, red deer, roe deer and badgers. it relies on active and passive survei ...201829543911
management of hunting waste as control measure for tuberculosis in wild ungulates in south-central spain.in recent decades, habitat change and the intensive management of wild ungulates for hunting have led to an increase in their populations in south-central spain. this implies a higher generation of hunting waste, which can favour the transmission of infectious diseases, including tuberculosis (tb). the aim of this study was to assess the usefulness of the proper disposal of hunting waste as tb control measure in wild boar (sus scrofa) and red deer (cervus elaphus) during the 2008/2009 to 2016/20 ...201829536638
mycobacterium caprae infection of red deer in western austria-optimized use of pathology data to infer infection dynamics.austria is officially bovine tuberculosis (tb) free, but during the last decade the west of the country experienced sporadic tb cases in cattle. free-ranging red deer are known to be the maintenance host of mycobacterium (m.) caprae in certain areas in austria, where cattle can become infected on alpine pastures shared with deer. the epidemiology of tb in deer in alpine regions is still poorly understood. to inform decisions on efficient interventions against tb in deer, a method is needed to be ...201830719435
investigations on the distribution of schistosoma turkestanicum skrjabin, 1913 (trematoda: schistosomatidae) infection of red deer in hungary and a combined method for the detection of s. turkestanicum eggs in droppings.additional geographical distribution of the central european populations of schistosoma turkestanicum and the detectability of their eggs in droppings were investigated in red deer samples, because this rare species had previously been shown only in a single hungarian habitat. samples from visceral organs, intestinal contents, and droppings on the ground from 11 hunting areas of hungary were investigated to find a new presence of this fluke. close to the first site of detection in the gemenc for ...201830580539
evaluating the individuality of animal-habitat relationships.examining the ways in which animals use habitat and select resources to satisfy their life history requirements has important implications for ecology, evolution, and conservation. the advent of radio-tracking in the mid-20th century greatly expanded the scope of animal-habitat modeling. thereafter, it became common practice to aggregate telemetry data collected on a number of tagged individuals and fit one model describing resource selection at the population level. this convention, however, ru ...201830519415
the european union summary report on surveillance for the presence of transmissible spongiform encephalopathies (tses) in 2017.this report presents the results of surveillance on transmissible spongiform encephalopathies (tses) in bovine animals, sheep, goats, cervids and other animal species, as well as genotyping in sheep, carried out in 2017 in the european union (eu) according to regulation (ec) 999/2001, and in iceland, norway and switzerland. in total, 1,312,714 cattle were tested by the 28 eu member states (mss) which is a decrease of 3% compared with 2016; 18,526 were tested by the three non-mss. for the first t ...201832625769
detection of seroconversion to bovine herpesvirus 1 related alphaherpesvirus and bovine viral diarrhea virusin polish free-living deer.there are several infectious agents of domestic cattle that can also be present in free-living ruminant populations. these include bovine herpesvirus 1 (bohv-1) and bovine viral diarrhea virus (bvdv) which are the causative agents of infectious bovine rhinotracheitis and bovine viral diarrhea, respectively. the study was conducted on serum samples from 59 red deer, 24 roe deer, and 3 fallow deer (86 in total), originating from two geographically separate areas of poland. the samples were tested ...201830468331
between-year vocal aging in female red deer (cervus elaphus).studying animal vocal aging has potential implication in the field of animal welfare and for modeling human voice aging. the objective was to examine, using a repeated measures approach, the between-year changes of weight, social discomfort score (bites of other hinds on hind pelt), body condition score (fat reserves) and acoustic variables of the nasal (closed-mouth) and the oral (open-mouth) contact calls produced by farmed red deer hinds (cervus elaphus) toward their young.201830333068
establishment of fascioloides magna in a new region of hungary: case report.during the monitoring of red deer (n = 124) and fallow deer (n = 13) populations in four neighbouring areas, the presence of fascioloides magna was confirmed in southwestern hungary. the prevalence and the mean intensity of the infection within the host populations ranged between 0 and 100% and 0-36.3, respectively. the determined prevalences are similar to that observed earlier in other european natural foci. the authors hypothesise that the appearance of f. magna in this region should have bee ...201830284615
pathological lesions in the lungs of red deer cervus elaphus (l.) induced by a newly-described dictyocaulus cervi (nematoda: trichostrongyloidea).the large lungworms of the genus dictyocaulus are causative agents of parasitic bronchitis in various ungulate hosts, including red deer. recently, the red deer-derived lungworm d. cervi was described and separated from d. eckerti. little is known of the transmission patterns, epidemiology, geographical distribution and pathogenicity of d. cervi. histological examinations were performed on 22 formalin-fixed lung tissue samples of hunted red deer. exclusively, d. cervi adults were derived from 15 ...201830253848
comparison of anti-inflammatory effect and protein profile between the water extracts from formosan sambar deer and red deer.velvet antler (va), the unossified antler from members of the family cervidae, has been used in traditional chinese medicines and health foods for over 2000 years in enhancement of kidney function and treatment or prevention of cardiovascular, immunological and gynaecological disease. the aim of this study was to investigate the anti-inflammatory effect of velvet antler water extracts from formosan sambar deer (rusa unicolor swinhoei, svae) and red deer (cervus elaphus, rvae). results indicated ...201830249326
estimating selection on the act of inbreeding in a population with strong inbreeding depression.inbreeding depression is widely regarded as a driving force in the evolution of dispersal, mate choice and sperm selection. however, due to likely costs of inbreeding avoidance, which are poorly understood, it is unclear to what extent selection to avoid inbreeding is expected in nature. moreover, there are currently very few empirical estimates of the strength of selection against the act of inbreeding (mating with a relative), as opposed to the fitness costs of being inbred. here, we use data ...201830230082
predation shapes the evolutionary traits of cervid weapons.sexually selected weapons evolved to maximize the individual reproductive success of males in many polygynous breeding species. many weapons are also retained outside of reproductive periods for secondary reasons, but the importance of these secondary functions is poorly understood. here we leveraged a unique opportunity from the predator-prey system in northern yellowstone national park, wy, usa to evaluate whether predation by a widespread, coursing predator (wolves) has influenced a specific ...201830177803
consumer preferences for red deer meat: a discrete choice analysis considering attitudes towards wild game meat and hunting.this study aims to analyse consumer preferences for red deer meat (rdm) (cervus elaphus) by conducting a case study in northern italy. this analysis considers how the attitudes of consumers towards wild game meat and hunting might influence such preferences. this goal is achieved by combining the results of a k-means clustering analysis of the attitudes collected by means of two valuation scales with a discrete choice experiment (ce). according to our results, a positive attitude towards wild ga ...201830173016
recolonizing gray wolves increase parasite infection risk in their prey.the recent recolonization of central europe by the european gray wolf (canis lupus) provides an opportunity to study the dynamics of parasite transmission for cases when a definitive host returns after a phase of local extinction. we investigated whether a newly established wolf population increased the prevalence of those parasites in ungulate intermediate hosts representing wolf prey, whether some parasite species are particularly well adapted to wolves, and the potential basis for such adapta ...201829468033
the prevalence of yersinia enterocolitica in game animals in poland.natural reservoirs of yersinia (y.) enterocolitica comprise different animal species, but little is known about the role of wild animals in the epidemiology of yersiniosis. the aim of the study was to evaluate the prevalence of y. enterocolitica among game animals in poland. the bio-serotypes and the pathogenicity markers of the analyzed isolates were determined. the experimental material comprised rectal swabs from 857 free-living animals hunter-harvested over a period of 2 years (2013-2014) in ...201829596492
histopathological, morphological, and molecular characterization of sarcocystis species in elk (cervus elaphus) from pennsylvania, usa.sarcocystis sarcocysts are common in many species of domestic and wild animals. here, we report sarcocystosis in muscles from 91 free range elk (cervus elaphus) from pennsylvania, usa, tested by histopathology, transmission electron microscopy (tem), and dna sequencing. sarcocysts were detected in hematoxylin and eosin (he)-stained sections from 83 of 91 (91.2%) elk, including 83/91 (91.2%) tongues and 15/17 (88.2%) hearts. with respect to age, sarcocysts were found in 0/5 calves, 8/9 (88.8%) ye ...201830120590
development of diagnostic snp markers to monitor hybridization between sika deer (cervus nippon) and wapiti (cervus elaphus).sika deer (cervus nippon) and wapiti (cervus elaphus) are closely related species and their hybridization can result in significant allele-shift of their gene pool. additive genetic effects and putative heterotic effects of their hybridization on growth performance could confer considerable economic advantage in deer farming. here, we used double-digest restriction site-associated dna sequencing technology (ddrad-seq) and detected ∼320,000 genome-wide snps from 30 captive individuals: 7 sika dee ...201829789312
comparative analysis of prions in nervous and lymphoid tissues of chronic wasting disease-infected cervids.the prevalence, host range and geographical bounds of chronic wasting disease (cwd), the prion disease of cervids, are expanding. horizontal transmission likely contributes the majority of new cwd cases, but the mechanism by which prions are transmitted among cwd-affected cervids remains unclear. to address the extent to which prion amplification in peripheral tissues contributes to contagious transmission, we assessed the prion levels in central nervous and lymphoreticular system tissues in whi ...201829580373
introgression of exotic cervus (nippon and canadensis) into red deer (cervus elaphus) populations in scotland and the english lake district.since the mid-19th century, multiple introductions of japanese sika deer (cervus nippon nippon) and north american wapiti (c. canadensis) have taken place in the british isles. while wapiti have generally been unsuccessful, sika have been very successful, especially in scotland where they now overlap at least 40% of the range of native red deer (c. elaphus). hybridization between these two species and red deer has been demonstrated in captivity and in the wild. using a panel of 22 microsatellite ...201829468030
sensitivity to antimicrobials of faecal buttiauxella spp. from roe and red deer (capreolus capreolus, cervus elaphus)detected with maldi-tof mass spectrometry.wild ruminants are an interesting topic for research because only limited information exists regarding their microbiota. they could also be an environmental reservoir of undesirable bacteria for other animals or humans. in this study faeces of the 21 free-living animals was sampled (9 cervus elaphus-red deer, adult females, 12 capreolus capreolus-roe deer, young females). they were culled by selective-reductive shooting during the winter season of 2014/2015 in the strzałowo forest district-piska ...201830468327
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