Publications

purification of a vasodilator-regulated phosphoprotein from human platelets.	cyclic-nucleotide-elevating vasodilators such as prostaglandin e1, prostacyclin, sodium nitroprusside and endothelium-derived relaxing factor inhibit both contraction of vascular smooth muscle cells and the aggregation of platelets at an early step of the activation cascade. previous studies from this laboratory [waldmann, r., nieberding, m. and walter, u. (1987) eur. j. biochem. 167, 441-448) established that in human platelets cyclic-nucleotide-elevating vasodilators stimulated a pattern of pr ...	1989	2806262
differential inhibition of downstream gene expression by the cauliflower mosaic virus 35s rna leader.	the effect of the 600 nucleotide-long camv 35s rna 5' leader sequence on the expression of downstream genes was analyzed both in plant protoplasts and in vitro. for transient expression studies in protoplasts derived from host and nonhost plants, the bacterial chloramphenicol acetyl transferase (cat) gene was fused to the initiation codon of orf vii. the leader sequence reduced cat expression two- to four-fold in protoplasts derived from three host species, but 10- to 50-fold in protoplasts deri ...	1989	2815595
differential inhibition of downstream gene expression by the cauliflower mosaic virus 35s rna leader.	the effect of the 600 nucleotide-long camv 35s rna 5' leader sequence on the expression of downstream genes was analyzed both in plant protoplasts and in vitro. for transient expression studies in protoplasts derived from host and nonhost plants, the bacterial chloramphenicol acetyl transferase (cat) gene was fused to the initiation codon of orf vii. the leader sequence reduced cat expression two- to four-fold in protoplasts derived from three host species, but 10- to 50-fold in protoplasts deri ...	1989	2815595
the measurement of secretory endometrial protein pp14 in serum from postmenopausal women receiving unopposed estrogen or continuously combined estrogen/progestogen.	a highly sensitive radioimmunoassay was developed to measure the low serum concentrations of the endometrial secretory placenta protein 14 (pp14) in postmenopausal women. the assay was established by selecting optimum reaction conditions for radioactive labelling of pp14, combined with simple procedures for the purification of the labelled pp14. the pp14 assay was used in 2 groups of healthy, early postmenopausal women blindly receiving either estrogen monotherapy (n = 20, placebo n = 25) or con ...	1989	2816479
on the estimation of pulmonary capillary pressure from arterial occlusion.	we have attempted to evaluate some approaches for estimating pulmonary capillary pressure from the transient pressure data obtained from the tip of the swanz-ganz catheter after inflation of the balloon. to this end experiments were carried out in anesthetized dogs in which pulmonary vasoconstriction was induced by infusion of histamine, serotonin, or norepinephrine or by hypoxia. in a group of closed chest dogs, the time course for the fall in the catheter tip pressure toward the wedge pressure ...	1989	2817586
[visualization of forensically important foreign bodies by direct roentgen magnification].	the detection of foreign bodies of microscopic dimensions with radiographic techniques is limited by the size and density of the particle and the radiographic resolution. these restrictions can be of decisive value in case work. the high grade direct radiographic magnification technique was successfully used in preceding experiments. due to its 1 mu focal spot, this tube allows direct magnifications up to 250 times. in present studies, the advantages as well as the limitations of this new techni ...	1989	2818477
[visualization of forensically important foreign bodies by direct roentgen magnification].	the detection of foreign bodies of microscopic dimensions with radiographic techniques is limited by the size and density of the particle and the radiographic resolution. these restrictions can be of decisive value in case work. the high grade direct radiographic magnification technique was successfully used in preceding experiments. due to its 1 mu focal spot, this tube allows direct magnifications up to 250 times. in present studies, the advantages as well as the limitations of this new techni ...	1989	2818477
periodontal sensory innervation of the dentition of the japanese shrew mole.	the neurohistological investigation of the periodontal sensory innervation in the whole dentition of the japanese shrew mole showed that the periodontium is much more densely supplied by the free nerve endings at the apical region than at the intermediate one at a ratio of about 8:1 in the upper dentition and 5:1 in the lower dentition. the interdental gingival tissue is innervated by the nerve plexus formed by the nerve fibers deriving mostly from the gingival and a few periodontal nerve fibers ...	1989	267109
[diagnosis and treatment of gastrointestinal polyps].		1989	259864
the dentist as a primary medical evaluator.		1989	267332
the analgesic effect of transcutaneous electrical nerve stimulation (tns) in patients with rheumatoid arthritis. a comparative study of different pulse patterns.	transcutaneous electrical nerve stimulation (tns) was used on 20 patients with severe wrist pain due to rheumatoid arthritis. three different stimulation frequencies were used: high 70 hz stimulation (70 tns), low frequency 3 hz stimulation (3 tns) and brief trains of stimuli with an internal frequency of 70 hz and with a repetition rate of 3 hz (3-70 tns). the analgesic effect was evaluated on the patient's own estimate of pain relief and by means of a loading test in which the length of time t ...	1989	313550
[spontaneous hematoma of the duodenum. apropos of 1 case--literature review].		1989	313491
[localized enamel opacity].		1989	287242
perioperative myocardial infarction associated with coronary artery bypass graft surgery: improved sensitivity in the diagnosis within 6 hours after operation with 99mtc-glucoheptonate myocardial imaging and myocardial-specific isoenzymes.	the present study was performed to evaluate scintigraphic imaging with technetium 99m-labeled glucoheptonate and serum enzyme levels of creatine phosphokinase isoenzyme (mb-cpk) in the early diagnosis of perioperative acute myocardial infarction associated with saphenous vein bypass graft operations. myocardial imaging was done in 27 patients (50% of whom were considered high-risk) before operation and again 5 hours after operation. four of these patients (15%) had both electrocardiographic and ...	1989	313189
hypercalciuria related to cadmium exposure.	a work force has been investigated for possible cadmium intoxication. one group who are coppersmiths have an 18.5 per cent prevalence of upper urinary tract stone disease associated with a statistically highly significant hypercalciuria and reduced serum inorganic phosphate. proof of exposure to cadmium has been confirmed in all workers. the trace element cadmium should be kept in mind when investigating stone formers who exhibit an unexplained hypercalciuria.	1989	209595
sickle cell crises: psychological factors associated with onset.		1989	267253
relationship of oral complications to peripheral blood leukocyte and platelet counts in patients receiving cancer chemotherapy.	patients undergoing cancer chemotherapy often suffer from oral complications as a result of their disease and its treatment. the effects of the chemotherapy on the bone marrow and oral mucosa, coupled with the patient's immunosuppressed state and altered oral microbial flora, predispose these patients to oral mucositis, infection, and hemorrhage. the oral mucosa appears to mirror the effects of the chemotherapy on the bone marrow, as there appears to be a direct relationship between the changing ...	1989	313547
studies on the prevalence of renal disease and hypertension in relation to schistosomiasis. iv. systemic blood pressure hypertension and related features.	two comparable rural communities but with varying endemicity of urinary schistosomiasis in environs of ibadan, nigeria, have been studied by a total cross-sectional population survey. it has been found that:- (i) schistosomiasis does not predispose to raised blood pressure (ii) both diastolic and systolic blood pressure increase with age. (iii) prevalence of systemic hypertension is higher in females than males (iv) body weight and height are related to hypertension in both males and females (v) ...	1990	313638
[genetic counseling of parents with children with clefts].	the elucidation of the aetiology of the individual case has proved to be of fundamental importance to the counselling interview as well as to the assessment of the risk for further affected children. up to now, a genetic cause in the form of chromosomal or gene mutations can be assumed only if the cleft is related to a distinct syndrome. an isolated cleft obviously owes its origin to a combination of genetic and extrinsic factors. consequently, the assessment of the risk of repetition is to a gr ...	1990	296849
antithrombotic therapy for coronary artery disease and valvular heart disease.		1990	2271788
the early periodic screening, diagnosis, and treatment program [epsdt]: status of progress and implementation in 51 states and territories.		1990	258722
a primer on labor unionism.		1990	258489
1. home treatment of hemophiliacs in france.		1990	268020
control of growth of benzo(a)pyrene-transformed 3t3 cells.	the growth controls observed in benzo[a]pyrene-transformed 3t3 cells (bp3t3) are compared with those of virus-transformed and normal 3t3 cells. superficially, the chemically transformed bp3t3 cells have the same behavior as virus-transformed sv3t3 cells. both grow to high cell density in culture medium with 10% serum, both form colonies in methocel, and both are tumorigenic. closer examination, however, has disclosed that bp3t3 cells exhibit "normal" growth controls at low serum concentrations. ...	1990	184467
disease: its cause and effect. 6: immunological diseases.		1990	258469
geriatric medicine: imperatives.		1990	267845
the structural basis of serological specificity in shigella flexneri o-antigens.		1990	1708342
distribution of insertion sequence is200 in salmonella and shigella.	two dna probes for the detection of insertion sequence is200 by either southern blotting or colony hybridization were constructed. one of the probes is a 300 bp ecori-hindiii fragment of is200 cloned onto pbluescript ks(+); the other is a tail-to-tail dimer of the same fragment cloned onto puc19. a survey of the presence of is200 among enteric bacteria revealed that more than 90% of the pathogenic or food-poisoning isolates of salmonella spp. examined contained one or more copies of insertion se ...	1990	1964174
h(+)-stimulated release of prostaglandin e2 and cyclic adenosine 3',5'-monophosphoric acid and their relationship to bone resorption in neonatal mouse calvaria cultures.	the addition of protons to the medium of neonatal mouse calvaria cultures stimulated bone resorption and release of calcium into the medium. in addition, added protons significantly increased the release of prostaglandin e2 (pge2) and cyclic adenosine 3',5'-monophosphoric acid (camp) from the bones. indomethacin significantly inhibited the release of calcium, pge2 and camp from proton-treated cultures. the positive control, parathyroid hormone (pth)-treated cultures, also gave rise to bone resor ...	1990	1964815
immunoglobulin profiles and antibacterial antibody levels in 50 patients a long time after posttraumatic splenectomy.	serum immunoglobulin and antibacterial antibody concentrations have been determined in 50 patients 2 to 20 years after posttraumatic splenectomy. the serum levels of igg, iga and igm were found to be significantly greater in the group of posttraumatic splenectomy patients than in that of the healthy controls. the serum ige concentrations did not differ statistically from that of the controls. the serum level of natural antibodies against intestinal bacteria were found to be elevated (shigella 1b ...	1990	1966943
close relationships between neopterin and beta-2-microglobulin levels in intravenous drug abusers.	serum from 36 intravenous drug abusers without acquired immunodeficiency syndrome (aids) or aids-related complex were tested for concentrations of neopterin and beta 2-microglobulin. the seroprevalence of human immunodeficiency virus (hiv) antibody in this group was 50%. previous studies of this group showed that the hiv antibody positive patients had significant increases in hla-dr expression on peripheral blood lymphocytes and increases in serum soluble cd8 antigen. both neopterin and beta 2-m ...	1990	1976599
competitive inhibition of magnesium-induced [3h]n-(1-[thienyl] cyclohexyl)piperidine binding by arcaine: evidence for a shared spermidine-magnesium binding site.	the polyamine competitive antagonist arcaine (1,4-diguanidino-butane) produced complete inhibition of basal [3h]n-(1-[thienyl] cyclohexyl)piperidine ([3h]tcp) binding, with an ic50 value of 4.52 +/- 0.93 microm. arcaine (5 and 10 microm) produced a decrease in the affinity without a significant change in the receptor density of [3h]tcp binding under equilibrium conditions. in addition, arcaine did not alter either n-methyl-d-aspartate-specific [3h] glutamate or strychnine-insensitive [3h]glycine ...	1990	1978243
[in vitro model for the analysis of the interaction between shigella flexneri and the intestinal epithelium].	an in vitro study of the adhesion and invasion of shigella flexneri was implemented, by means of incubation of laminary cuts of cecal mucosa of dunkin-hartley guinea pigs in a suspension of shigella flexneri, which was isolated from a patient with bacillary dysentery. the laminae were placed in plastic chambers for two hours at 37%c. after this the bacterial suspension was discarded so as to eliminate the bacilli which were not adhered. the epithelium was washed with saline and was processed for ...	1990	1669218
interferon production by shigella flexneri-infected fibroblasts depends upon intracellular bacterial metabolism.	the role of bacterial invasion and subsequent intracellular metabolism or replication, or both, in the induction of interferon (ifn) production in primary cultures of murine embryo fibroblasts (mefs) was examined. ifn production appeared to be dependent upon bacterial invasion. mefs that were challenged with shigella flexneri cultured at 30 degrees c to inhibit the temperature-dependent virulence gene expression that is essential for invasion failed to produce ifn. furthermore, inhibition of s. ...	1990	1688826
sequence and molecular characterization of a multicopy invasion plasmid antigen gene, ipah, of shigella flexneri.	a lambda gt11 expression library of tn5-tagged invasion plasmid pwr110 (from shigella flexneri serotype 5, strain m90t-w) contained a set of recombinants encoding a 60-kilodalton protein (designated ipah) recognized by rabbit antisera raised against s. flexneri invasion plasmid antigens (j. m. buysse, c. k. stover, e. v. oaks, m. m. venkatesan, and d. j. kopecko, j. bacteriol. 169:2561-2569, 1987). southern blot analysis of wild-type s. flexneri serotype 5 invasion plasmid dna (pwr100) digested ...	1990	1690703
bacteria-infected fibroblasts have enhanced susceptibility to the cytotoxic action of tumor necrosis factor.	the susceptibility of bacteria-infected fibroblasts to the cytotoxic action of tumor necrosis factor was investigated. l cells infected with shigella flexneri, salmonella typhimurium, or listeria monocytogenes, had an enhanced susceptibility to the cytotoxic activity of tnf-alpha. this enhanced susceptibility was dependent upon the challenge dose of bacteria, the concentration of tnf, and upon the exposure time of bacteria-infected cells to tnf. l cells infected with s. flexneri were susceptible ...	1990	1694886
[the significance of the o antigen for the diagnosis of acute sonne dysentery].	the authors investigated the clinical importance of determination of the o-antigen in coprofiltrates of patients with acute sonne dysentery by means of immunoenzymatic analysis. it was found that use of immunoenzymatic analysis is justified for etiological diagnosis of dysentery as well as with the purpose of forecasting recurrence of the disease.	1990	1697713
transient cytokeratin expression in skeletal muscle during murine embryogenesis.	cytokeratin expression was investigated in paravertebral skeletal musculature of 10 d and 18 d old embryos as well as in adult nmri-mice. the muscular nature of the evaluated tissue was evidenced by their expression of vimentin and desmin. binding moieties for cytokeratin antibodies (polyclonal and monoclonal) could be demonstrated only in muscle cells of 10 d old embryos. concerning subtypes, the mouse equivalents of the human cytokeratins nos. 8, 18 and 19 could be made probable. the importanc ...	1990	1698345
conduction velocity distribution of afferent fibers innervating the rat urinary bladder.	the conduction velocities of individual afferent fibers innervating the rat urinary bladder were determined by the antidromic stimulation of dorsal roots while recording from bladder postganglionic nerves. conduction velocities ranged from 0.5 to 21.0 m/s; 70% of the velocities were less than 2.5 m/s. the distribution within the dorsal roots was ipsilateral with 84% in l6 and 16% in s1. neuroanatomical tracing with horseradish peroxidase applied to individual bladder postganglionic nerves result ...	1990	1698508
desensitization of the dopaminergic system in bovine retina following incubation with high potassium.	the effect of potassium depolarization on dopamine d1 receptor activity in bovine retina was investigated. preincubation of bovine retinas in buffer containing high kcl (56 mm) as compared to a low kcl control buffer resulted in a significant decrease in dopamine-stimulated adenylate cyclase activity with no change in basal or gtp-stimulated adenylate cyclase activity. the apparent vmax for dopamine was decreased from 102 +/- 15 pmol/min/mg protein in retinas preincubated in high kcl to 71 +/- 1 ...	1990	1698510
monoclonal antibodies against an hla-b27-derived peptide react with an epitope present on bacterial proteins.	the role of molecular mimicry in the spondyloarthropathies was investigated with respect to the epitopes involved. mab were produced against a synthetic peptide whose sequence was derived from a polymorphic region of the hla-b27 molecule (amino acids 63-83). two antibodies (j7f2 and h2b6) were selected for study on the basis of their ability to react with bacterial envelope proteins (elisa) and b27-positive cells (immunofluorescence). j7f2 reacted preferentially with b27-positive cells and neith ...	1990	1698856
projection subtypes of rat neostriatal matrix cells revealed by intracellular injection of biocytin.	individual neostriatal-matrix spiny neurons were stained intracellularly with biocytin after intracellular recording in vivo, and their axons were traced into the globus pallidus (gp), entopeduncular nucleus (ep), and/or substantia nigra (sn). the locations of the neurons within the matrix compartment of the neostriatum (ns) were established by immunocytochemical counterstaining of sections containing the cell bodies using antibodies for calbindin-d28k. this allowed nearly complete visualization ...	1990	1698947
[the rapid diagnosis of salmonellosis by a determination of the antigen in the biosubstrates of patients using the coagglutination reaction].	the diagnostic potential of the coagglutination test was checked with the aim of improving the laboratory diagnosis of salmonella infections by the detection of salmonella specific antigen in different biological materials (feces, urine, saliva and immune complexes in blood sera). the study of all specimens resulted in the confirmation of the diagnosis in 78% of patients, often during the first days of the disease. the proportion of nonspecific reactions, as shown in the control groups of health ...	1990	1699371
demonstration of shared epitopes between bacterial proteins and hla class-i proteins using monoclonal antibodies.	monoclonal antibodies were used to identify the presence of epitopes on bacterial proteins which are cross-reactive with hla-b27. anti-hla-b27 monoclonals, b27m2 and ye-2 reacted with a major protein of approximately 35 kda, which was found to be ompa. on some strains a 19 kda protein was also seen. anti-shigella flexneri monoclonals were also developed which reacted with a 36 kda protein and a 19 kda protein. the 36 kda protein was ompf. these antibodies also reacted with a synthetic peptide re ...	1990	1701917
binding of enteric bacteria to hog gastric mucin.	the binding features of enteric bacteria were studied using a model mucin of hog gastric origin. the time requirement of binding is short, it is temperature-independent, but dose-dependent. the binding effectiveness of escherichia coli, shigella sonnei and shigella flexneri, as well as salmonella minnesota had a narrow range: 1.5-9 germs pro pg of mucin. the bacterial ligand of the binding is certainly not a polysaccharide as proved by the uniform binding of the r-mutant series of s. sonnei and ...	1990	1702253
sensitivity of shigella flexneri and escherichia coli bacteria to bacteriophages and to colicins, lost or established by the acquisition of r plasmids.	lac+ recombinant of shigella flexneri 2a carrying r-factors of the standard set have been constructed. the r+ transconjugants obtained have been compared with the original strain with respect to their susceptibility to a set of bacteriophages recommended for phagetyping of shigella strains and to a set of colicins. it have been found that as a result of acquiring some r-factors the susceptibility to bacteriophages changes; presence of certain r-factors causes susceptibility, that of other r-fact ...	1990	1715637
sensitivity of shigella strains to the bactericidal activity of human serum. ii. shigella flexneri 3 a is killed by complement activated via the classical pathway, with participation of lysozyme.	normal human serum is bactericidal for all studied strains (15) of shigella flexneri serotype 3a. the activity of the serum was similar irrespective of the invasiveness of the bacteria or its lack. the studied bacteria were susceptible to a single mechanism of bactericidal activity involving complement activated via the classical pathway, accompanied by the action of lysozyme.	1990	1715645
sensitivity of shigella strains to the bactericidal activity of human serum. iii. the diversity of activity variants of bactericidal serum factors against shigella flexneri serotypes.	normal human serum is bactericidal for all studied shigella flexneri strains (38) belonging to nine serotypes. six variants of bactericidal activity of serum factors for these bacteria were determined.	1990	1715646
[recurrent thromboembolism disclosing protein c deficiency. apropos of a case with familial investigation].	every thromboembolic manifestation, especially in young subjects, calls for an aetiological study in which haemostasis is evaluated primarily with assays of physiological coagulation inhibitors: protein c, protein s and antithrombin iii. protein c deficiency is found in 6 to 7% of thromboembolic manifestations. we report the case of a 21-year old man who had phlebitis followed by pulmonary embolism without facilitating factors. protein c level was 50% of normal value (0.50 iu/ml). the patient re ...	1990	2075365
[shigellosis].		1990	2076763
[the indices of pancreatic incretory function in acute intestinal infections].	results are reported of a study of the incretory function in 156 patients with acute intestinal infections. the incretory pancreatic function was found to be deranged in most patients in the acute period and tended to slow restoration during convalescence. this indicates the necessity of perfecting pathogenetic treatment of these patients and clinico-laboratory follow-up of patients with a history of acute intestinal infection with consideration of the incretory pancreatic function.	1990	2080556
[clinico-morphological comparisons in different types of dysentery].	clinical evidence and morphological picture of rectal biopsy specimens were analysed for 125 dysentery patients. as compared to a light course, moderately severe and severe dysentery presented enhanced microbial adherence and an elevated level of large lymphocytes in the external epithelium of the rectum, deeper penetration of shigella into crypts. on day 9-15 of the disease in attenuation of the clinical symptoms in severe and moderately dysentery morphological shifts persisted whereas the ligh ...	1990	2084341
specific binding of virg to the vir box requires a c-terminal domain and exhibits a minimum concentration threshold.	the positive regulatory protein virg from the virulence region of the ti plasmid of agrobacterium tumefaciens was first demonstrated to possess dna-binding capabilities using chromatographically purified protein and in vitro assays (powell et al., 1989). this paper is an extension of that research and presents evidence on the in vivo dna-binding properties of virg using a transcription interference assay. virg protein bound specifically to a 'vir box' response element and repressed transcription ...	1990	2089226
intraradicular bacteria and fungi in root-filled, asymptomatic human teeth with therapy-resistant periapical lesions: a long-term light and electron microscopic follow-up study.	light and electron microscopy were used to analyze nine therapy-resistant and asymptomatic human periapical lesions, which were removed as block biopsies during surgical treatment of the affected teeth. the cases that required surgery represented about 10% of all of the cases which received endodontic treatment and root fillings during the period 1977 to 1984. these cases revealed periapical lesions when they were examined 4 to 10 yr after treatment. the biopsies were processed for correlated li ...	1990	2094761
[the clinico-pathogenetic significance of disorders of the immune status in dysentery].	as many as 256 patients with flexner's dysentery caused by the same source of infection were examined. it has been established that in patients with a severe and protracted course of the disease, secondary immunodeficiency is formed by the relative hypersuppressor type and the function of phagocytizing macrophages decreases. patients with the reduced immune characteristics manifest deceleration of convalescence and repeated isolation of shigella after the antibiotic therapy. it is concluded that ...	1990	2094988
ultrastructural features of microbial colony organization.	the ultrastructure of microbial colonies was studied. inside the colonies three types of intercellular contacts were demonstrated. in the colonies of gram-negative bacteria, the cells were found to be connected by tight adhesions of outer membranes of the cell walls and membrane bridges. in the colonies of gram-positive bacteria, the intercellular contacts were formed by fusion of peptidoglucan layers of the cell walls. bacterial cells were differentiated by the presence of a capsule-like envelo ...	1990	2097346
potential shigella flexneri 2a and shigella sonnei i live vaccinal strains. characterization of immunogenicity in animal models.	rats and rabbits were immunized intraintestinally with different doses of virulent and nonvirulent live shigella flexneri 2a and shigella sonnei i strains. the nonvirulent strains had one or two attenuating markers. the period of excretion with the faeces of the bacteria and of their polysaccharide antigens, the proliferation of antigen-binding and antibody-producing cells in the spleens and gut-associated lymphoid tissues and the levels of antibodies in sera and faeces were studied. attenuated ...	1990	2099082
construction and evaluation of live attenuated vaccine strains of shigella flexneri and shigella dysenteriae 1.	shigellosis is an invasive disease of the human colon which is particularly prevalent among children of the developing world. no proper vaccine is available to protect against this enteric disease. it is currently accepted that only live strains with attenuated virulence administered orally may elicit protective immunity at the level of the colonic mucosa, which is the exclusive site of multiplication of causative microorganisms such as shigella flexneri and shigella dysenteriae 1. we have const ...	1990	2101481
hybrid vaccines using escherichia coli as an antigen carrier.		1990	2101482
[experience in using new drug preparations for treating adult patients with acute intestinal infections].	ciprofloxacin (bayer, frg), a derivative of hydroxyquinolone acid, was used for the treatment of patients with shigellosis and salmonellosis and for the sanitation of salmonella carriers. the drug turned out to exert a positive effect on bacteriological sanitation of the body. in order to treat patients with food toxinfection of unknown etiology, use was made of intetrix (farmacos, socialist federal republic of yugoslavia). inclusion of the drug into combined treatment of patients with food toxi ...	1990	2110389
molecular characterization of promoters of the lactococcus lactis subsp. cremoris temperate bacteriophage bk5-t and identification of a phage gene implicated in the regulation of promoter activity.	dna fragments from the temperate lactococcal bacteriophage bk5-t were cloned into the promoter-detecting plasmid pmu1328. five dna fragments conferring promoter activity were selected by transformation of streptococcus sanguis and were functional in escherichia coli, s. sanguis, and lactococcus lactis subspp. lactis and cremoris. the nucleotide sequences of these fragments were determined, and primer extension analysis was used to locate the site of initiation of transcription from each promoter ...	1990	2111118
electrolyte abnormalities associated with diarrhea in rhesus monkeys: 100 cases (1986-1987).	serum electrolyte values from 100 rhesus monkeys with diarrhea were reviewed. the most frequent finding was hyponatremia (88%), with hypochloremia next most frequently detected (80%). metabolic acidosis was less common (59%) and usually associated with high anion gap values. associations between electrolyte abnormalities and age, housing, or case outcome were not found. bacteriologic culturing was performed on fecal specimens from 90 monkeys. campylobacter coli or c jejuni alone was isolated fro ...	1990	2112122
phorbol myristate acetate inhibits hela 229 invasion by bordetella pertussis and other invasive bacterial pathogens.	the microfilament inhibitors cytochalasins b and d have been traditionally used to indirectly evaluate the requirement for actin in the uptake of invasive bacterial pathogens by nonprofessional phagocytes. through their effects on microfilaments, both cytochalasins also impart profound alterations in cellular morphology and surface topology, which likely interfere with adherence. alterations affecting adherence would complicate interpretation of the effect of cytochalasins on entry alone. as an ...	1990	2119340
[acute severe dyspnea as a side effect of drugs. report from the chdm (comprehensive hospital drug monitoring)].	this "syndrome" has been observed in 4 of 23,935 in-patients registered in the years 1974-1987 in the comprehensive hospital drug monitoring (bern/st. gallen), with 6 reactions. signs of an attack of bronchial asthma, laryngeal or pulmonary edema or a (heart-)circulatory event were not observed. each patient was cyanotic and 3 had the feeling of impending death. the eliciting drugs were penicillin-g (twice) and cefazolin (once), given i.v.; iron dextran i.m. (once); pitressin tannate i.m. (once) ...	1990	2120771
the two-component regulatory system ompr-envz controls the virulence of shigella flexneri.	in shigella flexneri, the ompb locus (containing the ompr and envz genes) was found to modulate expression of the vir genes, which are responsible for invasion of epithelial cells. vir gene expression was markedly enhanced under conditions of high osmolarity (300 mosm), similar to that encountered in tissues both extra- and intracellularly. two ompb mutants were constructed and tested for virulence and for osmotic regulation of vir genes. an envz::tn10 mutant remained invasive, although its viru ...	1990	2121709
growth inhibition of selected food-borne bacteria, particularly listeria monocytogenes, by plant extracts.	six extracts from chinese medicinal plants: tin men chu, sey lau pai, siu mao heung, bak tao yung, kam chin chiu and liao ya, were tested for their inhibitory effect on selected food-borne bacteria by the well assay technique. among them, tin men chu, siu mao heung and sey lau pai inhibited the growth of staphylococcus aureus, klebsiella pneumonia, escherichia coli, shigella flexneri, streptococcus faecalis, salmonella paratyphi, salm. enteritidis, enterobacter aerogenes, pseudomonas fluorescens ...	1990	2127264
cloning, sequencing and chromosomal assignment of a gene from saccharomyces cerevisiae which is negatively regulated by glucose and positively by lipids.	we report the molecular cloning, nucleotide (nt) sequence and chromosomal assignment of the saccharomyces cerevisiae gene glp1. this gene encoded a 15-kda protein that was synthesized at a low level during growth on glucose and was induced ninefold upon glucose deprivation. when glucose withdrawal was accompanied by the addition of fatty acids the induction was enhanced an additional two- to threefold. the glp1 gene product was identified as a soluble protein and purified using a combination of ...	1990	2129531
studies on nonspecific protection induced with omp of shigella flexneri and other genera of enterobacteriaceae.	outer membrane proteins (omp) isolated from shigella flexneri, escherichia coli, proteus vulgaris and salmonella typhimurium were tested for their protective activity. each omp preparate given to mice intraperitoneally in a single injection (5 micrograms/per mouse) protected the animals not only in homologous but also in varying intensity in heterologous systems. evidence was obtained that this nonspecific protection is cell mediated.	1990	2130800
immunogenic and protective properties of outer membrane proteins of hafnia alvei.	outer membrane proteins (omp) extracted from antigenically distinct or related strains of hafnia alvei containing defined composition of major proteins proved to be immunogenic. intraperitoneal immunization of mice with a single dose of such preparations protected the animals against homologous and heterologous hafnia strains. the omp preparations were also found to induce protection with varying intensity against escherichia, proteus, shigella and salmonella.	1990	2130804
a genetic therapy for familial hypercholesterolemia.	the whhl rabbit serves as an animal model for the human genetic disease, familial hypercholesterolemia. in initial studies aimed at the development of a genetic therapy for familial hypercholesterolemia (i.e., introduction of a normal ldl receptor gene), whhl rabbit skin fibroblasts were transduced with a retroviral vector expressing a normal human ldl receptor. correction of the whhl rabbit genetic defect in vitro was confirmed. autologous fibroblasts expressing ldl receptors were reimplanted i ...	1990	2132544
toxicity of certain pesticides against the chicken mite, dermanyssus gallinae.		1990	2132965
[preparation of microbial silage from fish of underutilized species].	fish silage was produced by microbial means from a mixture of several fish species which are not used for human consumption, and form part of the shrimp by-catch. the fish was mixed with a carbohydrate source (molasses) and a starter culture of lactobacillus plantarum 8014. the mixture was fermented at 30 +/- 2 degrees c. several tests were undertaken to determine the optimal conditions and concentration of molasses (5, 10 and 15%) and lactobacillus (0.5, 1.0, 2.5, 5.0 and 10.0%). results indica ...	1990	2134144
nucleotide sequence of the salmonella typhimurium glya gene.	the dna sequence of the salmonella typhimurium glya gene has been determined. the polypeptide deduced from the dna sequence contains 417 amino acids and has a calculated molecular weight of 45428 daltons. s1 nuclease mapping experiments located the transcription start point and possible transcription termination region. the nucleotide and amino acid sequences for the s. typhimurium and escherichia coli glya genes were compared. the nucleotide sequences show 89% identity, and the amino acid seque ...	1990	2134182
t cell activation. ii. activation of human t lymphoma cells by cross-linking of their mhc class i antigens.	the present work demonstrates that antibody-induced cross-linking of mhc class i antigens on jurkat t lymphoma cells leads to a rise in intracellular calcium (cai2+) and, in the presence of phorbol ester (pma), to il-2 production and il-2 receptor expression. the rise in cai2+ exhibited a profile very different from that obtained after anti-cd3 antibody-induced activation suggesting that activation signals are transduced differently after binding of anti-cd3 antibody and class i cross-linking, r ...	1990	2137376
regression of left ventricular hypertrophy in dialysis patients following correction of anemia with recombinant human erythropoietin.	left ventricular hypertrophy is an independent determinant of survival in patients with end-stage renal disease, and anemia is one determinant of hypertrophy in such patients. accordingly, the authors studied 22 dialysis patients by echocardiography before and after correction of their anemia with recombinant human erythropoietin. observers were blinded to patient identity and order of studies. hemoglobin increased from 63 +/- 8 g/l (mean +/- standard deviation) to 114 +/- 15 g/l; an increase of ...	1990	2138048
effects of chronic non-ketotic diabetes and aging on myocardial function and fatty acid oxidation.	the effects of aging and chronic non-ketotic diabetes on contractile properties, oxygen consumption, palmitate oxidation and morphology were studied in isolated, perfused working hearts of 2, 9, 12 and 22 month old rats. the heart rate, coronary flow, and oxygen consumption were no different among the 9, 12 and 22 month control and diabetic hearts. cardiac work was not depressed in control hearts until 22 months of age. depression of cardiac output due to aging in the control hearts progressed i ...	1990	2141842
[antibiotic resistance of shigella isolated in the kara-kalpak a.s.s.r. 1987-1988].	sensitivity of shigella spp. isolated in one of the hospitals of nukus within 1987-1988 and earlier in 1977 and 1985 was studied. s. flexneri 1-5 remained the main causative agents of dysentery on the territory. however, beginning from 1987 there were registered cases of dysentery caused by s. dysenteriae 1. the isolates were most sensitive to cefotaxime, cephaloridine, polymyxin b and gentamicin. the majority of the isolates were resistant to tetracycline, levomycetin (chloramphenicol) and stre ...	1990	2144726
[a case of group morbidity from reiter's syndrome in patients with a history of acute intestinal infection].		1990	2151299
inhibition of collagenolytic activity and metastasis of tumor cells by a recombinant human tissue inhibitor of metalloproteinases.	metalloproteinases secreted by tumor cells play an important role in metastasis. in the present study, we determined whether an inhibitor of these proteinases could inhibit the ability of tumor cells to degrade collagen and to metastasize. metalloproteinases with degradative activities for type i collagen, type iv collagen, gelatin, and casein were secreted by a highly metastatic rat embryo cell line (4r) transfected by c-ha-ras1 (also known as hras1). these metalloproteinases were identified by ...	1990	2156082
simultaneous primary infection with hiv and cmv leading to severe pancytopenia, hepatitis, nephritis, perimyocarditis, myositis, and alopecia totalis.	simultaneous primary infection with hiv and cmv in an 18-year-old woman led to an acute cytotoxic reaction, manifesting as pancytopenia, hepatitis, nephritis, perimyocarditis, and myositis. within 14 days parameters indicating acute cell damage reverted to normal. two weeks later transient alopecia totalis developed. initially, hiv-antigen but no hiv antibodies were present. within 3 weeks hiv-igg antibodies appeared while hiv antigen disappeared. anti-cmv-igm was clearly and anti-cmv-igg questi ...	1990	2156107
mri quantitation of edema in focal cerebral ischemia in cats: correlation with cytochrome aa3 oxidation state.	1h mri permits detection of edema in the brain. in a middle cerebral artery stroke model in the cat, we found a significant correlation between an edema index based on mri and a sensitive metabolic index of ischemia, the in vivo oxidation status of mitochondrial cytochrome aa3 determined by near-infrared reflectance spectrophotometry (r = -0.70, alpha = 0.001). this result suggests that a simple, noninvasive study using mri can provide an index of the extent of ischemic damage in an experimental ...	1990	2156126
molecular characterization of a trans-acting, positive effector (ipar) of invasion plasmid antigen synthesis in shigella flexneri serotype 5.	a trans-acting, positive effector of invasion plasmid antigen (ipa) synthesis has been identified and mapped on the pwr100 invasion plasmid of shigella flexneri serotype 5 (strain m90t-w). recombinant plasmids carrying this regulatory gene, designated ipar, were found to restore full virulence to a non-invasive ipar::tn5 insertion mutant [m90t-w(phs1042)] that had lost the ability to synthesize four ipa antigens (ipaa, 70 kda; ipab, 62 kda; ipac, 42 kda; and ipad, 37 kda). genetic mapping of the ...	1990	2166210
coxsackie b1 virus infection enhances the bacterial invasiveness, the phagocytosis and the membrane permeability in hep-2 cells.	to analyze the effect of coxsackie b1 virus infection on bacterial invasiveness, phagocytosis and cytoplasma membrane permeability, we have studied invasiveness of shigella flexneri, unspecific phagocytosis of latex particles and release of the non-metabolizible amino acid, alpha-aminoisobutyric acid (aib). virus infection enhanced invasiveness of s. flexneri and phagocytosis of latex beads and increased plasma membrane permeability as measured by release of aib. the effect on all three function ...	1990	2166540
morphine and fentanyl anesthetic interactions with diazepam: relative antagonism in rats.	the anesthetic effects of morphine-diazepam and fentanyl-diazepam combinations as characterized by abolition of the movement response to noxious stimulation were studied in rats to test the hypothesis of antagonistic interactions between the components of these combinations. noxious pressure on the tail was used to induce the response. dose-effect curves were constructed for the drugs given alone and in combination. with the use of probit procedure ed50 values for single drugs and their combinat ...	1990	2168134
kinetics of the reaction of prostaglandin h synthase compound ii with ascorbic acid.	the reduction of prostaglandin h synthase compound ii by ascorbic acid in the presence of diethyldithiocarbamate was studied in 0.1 m phosphate buffer (ph 8.0) at 4.0 +/- 0.5 degrees c, by rapid scan spectrometry and transient state kinetics. a saturation effect and nonzero intercept were observed in the plot of pseudo-first-order rate constant versus ascorbic acid concentration. the saturation behavior suggests formation of a complex between prostaglandin h synthase compound ii and ascorbic aci ...	1990	2168160
anti-viral immunity induced by recombinant nucleoprotein of influenza a virus. iii. delivery of recombinant nucleoprotein to the immune system using attenuated salmonella typhimurium as a live carrier.	a plasmid encoding the influenza nucleoprotein gene from a/nt/60/68 virus was transduced into the attenuated salmonella typhimurium aroa- strain sl3261. the bacterial vector expressing the viral gene product was able to induce both humoral and cell-mediated immune responses to the nucleoprotein antigen. cd4+ virus-specific t cells capable of proliferation were readily induced and, in some circumstances, class ii major histocompatibility complex (mhc)-restricted cytotoxicity was detected. however ...	1990	2168340
rapid detection of herpes simplex virus in clinical samples by flow cytometry after amplification in tissue culture.	murine monoclonal antibodies (mabs) against herpes simplex virus type 1 and 2 (hsv-1 and -2, respectively) nuclear antigens were used to identify cells infected with hsv-1 or -2 by indirect immunofluorescence in conjunction with flow cytometry after virus amplification of mrc-5 cell monolayers. the results indicate that mabs q1, q2, and h-640 detect hsv-1- and hsv-2-infected cells, mab sd-1 detects hsv-2- but not hsv-1-infected cells, and mab 58-s detects hsv-1- but not hsv-2-infected cells. mab ...	1990	2168440
modulation of experimentally induced epilepsy by intracerebral grafts of fetal gabaergic neurons.	systemic administration of pilocarpine to rats induces seizures that resemble complex partial epilepsy in humans. susceptibility to these seizures is increased by lesion of the gabaergic striatonigral projection. transplantation of fetal gabaergic neurons, but also of control non-gabaergic tissue, to the deafferent substantia nigra can reduce such lesion-increased seizure susceptibility. these observations are consistent with prior evidence that gabaergic basal ganglia outflow plays an important ...	1990	2168529
molecular mechanisms of glial cell swelling in acidosis.	the mechanism of glial swelling in acidosis were investigated in an in vitro model. c6 glial cells were exposed to ph levels between 7.4 and 6.2. the cell volume response was determined by flow cytometry. cell swelling of 110% to 115% was observed if ph was reduced to 6.8 or below. this effect was independent of the length of exposure to acidosis. swelling induced by ph could be attenuated by (a) inhibition of the na+/h+ antiporter by amiloride, (b) replacement of bicarbonate by hepes, and (c) i ...	1990	2168665
antiinflammatory properties of the new antirheumatic agent 4-acetylaminophenylacetic acid.	the antiinflammatory activity of 4-acetylaminophenylacetic acid (ms-932) was investigated. ms-932 did not suppress the acute inflammation of carrageenin-induced paw edema in rats or of primary swelling in adjuvant arthritic rats. however, prophylactic treatment with ms-932 inhibited secondary inflammation in adjuvant arthritic rats. ms-932 also restored to normal the weight of the spleen and the serum albumin/globulin ratio of adjuvant arthritic rats. in addition to its prophylactic effect, ms-9 ...	1990	2168705
photoprotective effect of superoxide-scavenging antioxidants against ultraviolet radiation-induced chronic skin damage in the hairless mouse.	albino hairless mice (skh:hr-1) exposed chronically to suberythemal doses of ultraviolet radiation develop visible skin changes, histological alterations, and tumors. topical treatment of mice with solutions of superoxide-scavenging antioxidants (such as alpha-tocopherol, ascorbic acid, propyl gallate and trolox) prior to each uvb radiation exposure reduced significantly the severity of these events. tocopherol esters and ascorbyl palmitate were not as effective as the parent compounds in provid ...	1990	2169296
inactivation of beta-lactamase from shigella flexneri ucsf-129 by 6-beta-iodopenicillanic acid: classification of the enzyme.	the beta-lactamase from shigella flexneri ucsf-129 was irreversible inactivated by 6-beta-iodopenicillanic acid. only one serine residue was modified, according to the spectra change and the amino acid analyses. a ph variation of 0.3 units was found when the chemically modified enzyme was submitted to isoelectric focusing. the inactivation constant of the fast time course reaction was 0.1 seg-1. protection of 96% was obtained, using cephradine 2,830 times more concentrated than 6-beta-iodopenici ...	1990	2170824
prevalence of human papillomaviruses in cervical scrapings of unselected women.		1990	2171941
a nonvirulent mutant of listeria monocytogenes does not move intracellularly but still induces polymerization of actin.	listeria monocytogenes has the capacity to penetrate and multiply within professional and nonprofessional phagocytic cells, such as the caco-2 human enterocytelike cell line. it was shown recently that shortly after listeriae have been phagocytosed, the phagosomal membrane is dissolved, probably by the action of the bacterial cytolysin listeriolysin o. the listeriae, which are then lying obviously free in the cytoplasm, become surrounded by a coat of actin filaments within a few hours. once form ...	1990	2172164
expression of major histocompatibility complex antigens on inflammatory peripheral nerve lesions.	the expression of major histocompatibility complex (mhc) antigens by cells of the rat peripheral nervous system (pns) was studied using a model of peripheral nerve transplantation. monoclonal antibodies to polymorphic determinants of mhc class i and class ii (ia) molecules were used to determine donor or recipient origin of mhc antigen-bearing cells in nerve allografts. the expression of class i and class ii antigens by pns parenchymal cells was modified during varying alloimmune conditions. bas ...	1990	2172305
biphasic effects of polarizing current on adenosine-sensitive generation of cyclic amp in rat cerebral cortex.	cyclic amp accumulation elicited by adenosine was investigated in cortical slices of rats following the application of a weak anodal direct current (anodal polarization) to the unilateral sensorimotor cortex. anodal polarization with 3.0 microa for 30 min caused an increase in the adenosine-elicited accumulation of cyclic amp in the polarized cortex, in which case the increase in the polarized cortical region was highlighted by repeated applications of the currents. polarization with 0.3 microa ...	1990	2173816
human pancreatic islet cell specific 38 kilodalton autoantigen identified by cytomegalovirus-induced monoclonal islet cell autoantibody.	our previous finding that about 15% of newly diagnosed patients with type 1 (insulin-dependent) diabetes mellitus had human cytomegalovirus genome in their lymphocytes and islet cell autoantibodies in their sera, suggests that autoimmune type 1 diabetes is associated with persistent cytomegalovirus infection under certain circumstances. this investigation was initiated to see if cytomegalovirus can induce islet cell autoantibodies and if the autoantibodies react with any specific islet protein(s ...	1990	2174808
sequencing and enhanced expression of the gene encoding diadenosine 5',5'''-p1, p4-tetraphosphate (ap4a) phosphorylase in saccharomyces cerevisiae.	the gene, dtp, coding for diadenosine 5',5'''-p1, p4-tetraphosphate (ap4a) phosphorylase was isolated from a saccharomyces cerevisiae genomic dna library in lambda gt11. in yeast and escherichia coli transformed with the multicopy vector, yep352, containing the cloned dtp gene, the ap4a phosphorylase was produced at levels nine- to 17-fold higher than in untransformed hosts. the nucleotide (nt) sequence was determined. the gene codes for a polypeptide chain of 321 amino acids (aa). two-aa sequen ...	1990	2174812
depletion of brain alpha-msh alters prostaglandin and interleukin fever in rats.	alpha-melanocyte stimulating hormone (alpha-msh), a putative endogenous antipyretic agent, is synthesized largely within neurons in the arcuate nucleus. to test the hypothesis that destruction of this area would increase the febrile response, male wistar rats, treated as neonates with intraperitoneal injections of monosodium glutamate (msg) or saline, were given intracerebroventricular (i.c.v.) injections of prostaglandin e1 (20 ng; 200 ng) or purified interleukin-1 (20 u) and body temperature w ...	1990	2175244
live shigella flexneri 2a and shigella sonnei i vaccine candidate strains with two attenuating markers. i. construction of vaccine candidate strains with retained invasiveness but reduced intracellular multiplication.	live shigella flexneri 2a and shigella sonnei phase i vaccine candidate strains with two virulence-reducing markers were constructed through stepwise incorporation of weakly attenuating purine auxotrophy with subsequent rifampicin-resistance (rna polymerase) mutation to yield optimal attenuation. these vaccine candidate strains showed an unaltered plasmid profile; did not cause keratoconjunctivitis in the sereny test, while being excreted for a short but still marked period and providing partial ...	1990	2180230