Publications

exploration of sequence space as the basis of viral rna genome segmentation.	the mechanisms of viral rna genome segmentation are unknown. on extensive passage of foot-and-mouth disease virus in baby hamster kidney-21 cells, the virus accumulated multiple point mutations and underwent a transition akin to genome segmentation. the standard single rna genome molecule was replaced by genomes harboring internal in-frame deletions affecting the l- or capsid-coding region. these genomes were infectious and killed cells by complementation. here we show that the point mutations i ...	2014	24757055
single amino acid substitution of vp1 n17d or vp2 h145y confers acid-resistant phenotype of type asia1 foot-and-mouth disease virus.	infection by foot-and-mouth disease virus (fmdv) is triggered by the acidic ph in endosomes after virus uptake by receptor-mediated endocytosis. however, dissociation of the fmdv 146s particle in mildly acidic conditions renders inactivated foot-and-mouth disease (fmd) vaccines much less effective. type asia1 fmdv mutants with increased resistance to acid inactivation were selected to study the molecular basis of viral resistance to acid-induced disassembly and improve the acid stability of fmdv ...	2014	24752763
molecular biological characteristics of foot-and-mouth disease virus in the african buffalo in southern africa.		2014	28235272
spatial and temporal distribution of foot-and-mouth disease virus in the eastern zone of tanzania.		2014	28235270
the changing landscape of the molecular epidemiology of foot-and-mouth disease virus in southern africa north of limpopo and east africa.		2014	28235269
full genome sequencing to study the evolutionary characteristics of foot-and-mouth disease virus in southern africa.		2014	28235268
screening for foot-and-mouth disease virus in livestock-wildlife interface areas of tanzania.		2014	28235267
evaluation of monoclonal antibody-based sandwich direct elisa (msd-elisa) for antigen detection of foot-and-mouth disease virus using clinical samples.	a monoclonal antibody-based sandwich direct elisa (msd-elisa) method was previously developed for foot-and-mouth disease (fmd) viral antigen detection. here we evaluated the sensitivity and specificity of two fmd viral antigen detection msd-elisas and compared them with conventional indirect sandwich (is)-elisa. the msd-elisas were able to detect the antigen in saliva samples of experimentally-infected pigs for a longer term compared to the is-elisa. we also used 178 rt-pcr-positive field sample ...	2014	24736560
serological evidence indicates that foot-and-mouth disease virus serotype o, c and sat1 are most dominant in eritrea.	foot-and-mouth disease (fmd) is endemic in eritrea and in most parts of africa. to be able to control fmd using vaccination, information on the occurrence of various foot-and-mouth disease serotypes in eritrea is needed. in this cross-sectional study, 212 sera samples were collected from fmd infected and recovered animals in eritrea. these samples were tested for the presence of antibodies against fmd non-structural proteins (nsp) and neutralizing antibodies against six of the seven (all but sat ...	2014	23480728
proof of concept for the inhibition of foot-and-mouth disease virus replication by the anti-viral drug 2'-c-methylcytidine in severe combined immunodeficient mice.	recent european contingency plans envisage emergency vaccination as an animal-friendly control strategy for foot-and-mouth disease (fmd). anti-viral drugs may be used as an alternative or complementary measure. we here demonstrate that the nucleoside analogue 2'-c-methylcytidine (2'cmc) protects severe combined immunodeficient (scid) mice against lethal fmd virus infection. in brief, scid mice were inoculated with serotype a fmd virus and treated for five consecutive days with 2'cmc. all 15 trea ...	2014	23480064
foot and mouth disease virus-loaded fungal chitosan nanoparticles for intranasal administration: impact of formulation on physicochemical and immunological characteristics.	nasal vaccination is a promising, needle-free alternative route for parenteral vaccination. this study introduces a simple, scalable nasal vaccine delivery formulation for foot and mouth disease virus (fmdv) using chitosan (cs) nanoparticles and assesses the potential of fungal cs for use as nanocarriers for mucosal vaccines. fungal cs was extracted from fungal biomass and physiochemically characterized. fmdv-loaded cs nanoparticles, prepared using an ionic gelation technique, were characterized ...	2014	23590209
epidemiological analysis, serological prevalence and genotypic analysis of foot-and-mouth disease in nigeria 2008-2009.	the epidemiological situation of foot-and-mouth disease virus (fmdv) is uncertain in nigeria, where the disease is endemic, and the majority of outbreaks are unreported. control measures for fmd in nigeria are not being implemented due to the absence of locally produced vaccines and an official ban on vaccine importation. this study summarizes the findings of a 3-year study aimed at quantifying the seroprevalence of fmd, its distribution in susceptible species and the genetic diversity of fmdv i ...	2014	23347819
genetic basis of antigenic variation in foot-and-mouth disease serotype a viruses from the middle east.	foot-and-mouth disease viruses (fmdv) from serotype a exhibit high antigenic diversity. within the middle east, a strain called a-iran-05 emerged in 2003, and subsequently replaced the a-iran-96 and a-iran-99 strains that were previously circulating in the region. viruses from this strain did not serologically match with the established a/iran/96 vaccine, although most early samples matched with the older a22/iraq vaccine. however, many viruses from this strain collected after 2006 had poor sero ...	2014	24035435
outbreaks and diagnosis of foot-and-mouth disease serotype o in the republic of korea, april-june 2010.	thirteen outbreaks of foot-and-mouth disease (fmd) were reported in pigs and cattle in korea between 8 april and 4 june 2010. the fmd virus (fmdv) isolates were of serotype o, indicating that they were related to the virus strains of the southeast asia topotype that are circulating in east asian countries. animals carrying the viruses were identified by reverse transcriptase-polymerase chain reaction (rt-pcr) during a 29-day period between 8 april and 6 may, 2010. prior to this outbreak, these f ...	2014	23164336
production and characterization of single-chain antibody (scfv) against 3abc non-structural protein in escherichia coli for sero-diagnosis of foot and mouth disease virus.	differentiation of foot-and-mouth disease infected from vaccinated animals is essential for effective implementation of vaccination based control programme. detection of antibodies against 3abc non-structural protein of fmd virus by immunodiagnostic assays provides reliable indication of fmd infection. sero-monitoring of fmd in the large country like india is a big task where thousands of serum samples are annually screened. currently, monoclonal or polyclonal antibodies are widely used in these ...	2014	25439091
in vitro evolution and affinity-maturation with coliphage qβ display.	the escherichia coli bacteriophage, qβ (coliphage qβ), offers a favorable alternative to m13 for in vitro evolution of displayed peptides and proteins due to high mutagenesis rates in qβ rna replication that better simulate the affinity maturation processes of the immune response. we describe a benchtop in vitro evolution system using qβ display of the vp1 g-h loop peptide of foot-and-mouth disease virus (fmdv). dna encoding the g-h loop was fused to the a1 minor coat protein of qβ resulting in ...	2014	25393763
[enhancement of mouse immune response by recombinant adenovirus co-expressing vp1-2a of foot-and-mouth disease virus and porcine ifn-α].	to construct the recombinant adenovirus co-expressing foot-and-mouth disease virus (fmdv) viral structural protein1-2a (vp1-2a) and porcine interferon-α (poifn-α), and study its impact on mouse adaptive immune responses.	2014	25374073
development of a blocking elisa based on a monoclonal antibody against a predominant epitope in non-structural protein 3b2 of foot-and-mouth disease virus for differentiating infected from vaccinated animals.	a monoclonal antibody (mcab) against non-structural protein (nsp) 3b of foot-mouth-disease virus (fmdv) (3b4b1) was generated and shown to recognize a conserved epitope spanning amino acids 24-32 of 3b (gpyagpmer) by peptide screening elisa. this epitope was further shown to be a unique and predominant b cell epitope in 3b2, as sera from animals infected with different serotypes of fmdv blocked the ability of mcab 3b4b1 to bind to nsp 2c3ab. also, a polyclonal antibody against nsp 2c was produce ...	2014	25369323
rapid isolation of antibody from a synthetic human antibody library by repeated fluorescence-activated cell sorting (facs).	antibodies and their derivatives are the most important agents in therapeutics and diagnostics. even after the significant progress in the technology for antibody screening from huge libraries, it takes a long time to isolate an antibody, which prevents a prompt action against the spread of a disease. here, we report a new strategy for isolating desired antibodies from a combinatorial library in one day by repeated fluorescence-activated cell sorting (facs). first, we constructed a library of sy ...	2014	25303314
development of anti-bovine iga single chain variable fragment and its application in diagnosis of foot-and-mouth disease.	recombinant antibody fragments like single chain variable fragments (scfvs) represent an attractive yet powerful alternative to immunoglobulins and hold great potential in the development of clinical diagnostic/therapeutic reagents. structurally, scfvs are the smallest antibody fragments capable of retaining the antigen-binding capacity of whole antibodies and are composed of an immunoglobulin (ig) variable light (vl) and variable heavy (vh) chain joined by a flexible polypeptide linker. in the ...	2014	24678404
influence of hydrophilic amino acids and gc-content on expression of recombinant proteins used in vaccines against foot-and-mouth disease virus in escherichia coli.	epitope-based protein expression in escherichia coli can be improved by adjusting its amino acid composition and encoding genes. to that end, we analyzed 24 recombinant epitope proteins (reps) that carry multiple epitopes derived from vp1 protein of foot-and-mouth disease virus. high level expression of the reps was attributed to a high content of arg, asn, asp and thr, a low content of gln, pro and lys, a high content of hydrophilic amino acids and a higher isoelectric point value resulting fro ...	2014	24375229
comparison of stabilisers for development of a lyophilised multiplex reverse-transcription pcr mixture for rapid detection of foot and mouth disease virus serotypes.	multiplex reverse-transcription polymerase chain reaction (mrt-pcr) assay is a sensitive and rapid method for the detection and serotyping of foot and mouth disease virus (fmdv). however, the method has not been used to its full potential, because of factors such as cost, a lack of infrastructure and the complexity of the reaction mixture. this study was undertaken to optimise and validate a thermostable, lyophilised, ready-to-use mrt-pcr kit for the rapid detection of fmdv in field laboratories ...	2014	25812209
induction of mucosal immune responses and protection of cattle against direct-contact challenge by intranasal delivery with foot-and-mouth disease virus antigen mediated by nanoparticles.	the aim of this study was to enhance specific mucosal, systemic, and cell-mediated immunity and to induce earlier onset of protection against direct-contact challenge in cattle by intranasal delivery of a nanoparticle-based nasal vaccine against type a foot-and-mouth disease (fmd). in this study, two kinds of nanoparticle-based nasal vaccines against type a fmd were designed: (1) chitosan-coated poly(lactic-co-glycolic acid) (plga) loaded with plasmid dna (chi-plga-dna) and (2) chitosan-trehalos ...	2014	25506214
swine leukocyte antigen (sla) class i allele typing of danish swine herds and identification of commonly occurring haplotypes using sequence specific low and high resolution primers.	the swine major histocompatibility complex (mhc) genomic region (sla) is extremely polymorphic comprising high numbers of different alleles, many encoding a distinct mhc class i molecule, which binds and presents endogenous peptides to circulating t cells of the immune system. upon recognition of such peptide-mhc complexes (pmhc) naïve t cells can become activated and respond to a given pathogen leading to its elimination and the generation of memory cells. hence sla plays a crucial role in main ...	2014	25457547
evolutionary analysis and prediction of peptide vaccine candidates for foot-and-mouth-disease virus types a and o in bangladesh.	foot-and-mouth disease (fmd), an endemic disease of cloven-hoofed animals, causes an annual economic loss of us$60-150 million in bangladesh. there is no cross-protection among the foot-and-mouth disease virus (fmdv) serotypes and vaccination escape mutation may happen. peptide vaccine is a safer alternative. the aim of this study is to predict and map the b and t cell epitopes of vp1 proteins of fmdv serotypes o and a that were circulating in bangladesh from 2011 to 2013. using evolutionary and ...	2014	25452681
a universal protocol to generate consensus level genome sequences for foot-and-mouth disease virus and other positive-sense polyadenylated rna viruses using the illumina miseq.	next-generation sequencing (ngs) is revolutionizing molecular epidemiology by providing new approaches to undertake whole genome sequencing (wgs) in diagnostic settings for a variety of human and veterinary pathogens. previous sequencing protocols have been subject to biases such as those encountered during pcr amplification and cell culture, or are restricted by the need for large quantities of starting material. we describe here a simple and robust methodology for the generation of whole genom ...	2014	25269623
identification and analysis of differential mirnas in pk-15 cells after foot-and-mouth disease virus infection.	the alterations of micrornas(mirnas) in host cell after foot-and-mouth disease virus (fmdv) infection is still obscure. to increase our understanding of the pathogenesis of fmdv at the post-transcriptional regulation level, solexa high-throu micrornas (mirnas) play an important role both in the post-transcriptional regulation of gene expression and host-virus interactions. despite investigations of mirna expression ghput sequencing and bioinformatic tools were used to identify differentially exp ...	2014	24675746
development and evaluation of a one step reverse transcription-loop mediated isothermal amplification assay (rt-lamp) for rapid detection of foot and mouth disease virus in india.	a simple, rapid and sensitive diagnostic assay for foot-and-mouth disease (fmd) is required for deployment in the field. in this study, development of reverse transcription-loop mediated isothermal amplification (rt-lamp) assay based on the 3d polymerase gene for specific and rapid detection fmd virus (fmdv) was carried out. the assay was optimised with viral rna extracted from serotype o, a and asia 1 fmdv vaccine strains, which resulted a reliable amplification at 65 °c for 60 min. the amplifi ...	2014	25674604
expression of cry1ab and cry2ab by a polycistronic transgene with a self-cleavage peptide in rice.	insect resistance to bacillus thuringiensis (bt) crystal protein is a major threat to the long-term use of transgenic bt crops. gene stacking is a readily deployable strategy to delay the development of insect resistance while it may also broaden insecticidal spectrum. here, we report the creation of transgenic rice expressing discrete cry1ab and cry2ab simultaneously from a single expression cassette using 2a self-cleaving peptides, which are autonomous elements from virus guiding the polycistr ...	2014	25333312
inducible expression of a fusion gene encoding two proteinase inhibitors leads to insect and pathogen resistance in transgenic rice.	plant proteinase inhibitors (pis) are considered as candidates for increased insect resistance in transgenic plants. insect adaptation to pi ingestion might, however, compromise the benefits received by transgenic expression of pis. in this study, the maize proteinase inhibitor (mpi), an inhibitor of insect serine proteinases, and the potato carboxypeptidase inhibitor (pci) were fused into a single open reading frame and introduced into rice plants. the two pis were linked using either the proce ...	2014	24237606
complex assembly, crystallization and preliminary x-ray crystallographic analysis of the bovine cd8αα-bola-2*02201 complex.	in order to clarify the structural characteristics of the bovine mhc class i molecule (bola-i) complexed with cd8αα (cd8αα-bola-i), bovine cd8αα, bola-i (bola-2*02201) and β2m were expressed and purified, and were then assembled with a peptide derived from foot-and-mouth disease virus (fmdv-vp1yy9) and crystallized. the crystal diffracted to 1.7 å resolution and belonged to space group p21, with unit-cell parameters a=53.9, b=103.8, c=61.8 å, α=γ=90, β=96°. the asymmetric unit contained one comp ...	2014	24915083
first finding of southeast asia topotype of foot-and-mouth disease virus in kinmen, taiwan, in the 2012 outbreak.	foot-and-mouth disease virus, a member of genus aphthovirus within the family picornaviridae, affects cloven-hoofed animals, causing foot-and-mouth disease characterized by vesicle development. the southeast asia topotype, one of the topotypes within serotype o of the virus, is prevalent in some asian countries, but had not previously been found in taiwan. the topotype was first found in pigs in kinmen island, taiwan, in 2012 and identified by nucleotide sequence comparison and phylogenetic anal ...	2014	25056674
laboratory animal models to study foot-and-mouth disease: a review with emphasis on natural and vaccine-induced immunity.	laboratory animal models have provided valuable insight into foot-and-mouth disease virus (fmdv) pathogenesis in epidemiologically important target species. while not perfect, these models have delivered an accelerated time frame to characterize the immune responses in natural hosts and a platform to evaluate therapeutics and vaccine candidates at a reduced cost. further expansion of these models in mice has allowed access to genetic mutations not available for target species, providing a powerf ...	2014	25000962
3cpro of foot-and-mouth disease virus antagonizes the interferon signaling pathway by blocking stat1/stat2 nuclear translocation.	foot-and-mouth disease virus (fmdv) causes a highly contagious, debilitating disease in cloven-hoofed animals with devastating economic consequences. to survive in the host, fmdv has evolved to antagonize the host type i interferon (ifn) response. previous studies have reported that the leader proteinase (l(pro)) and 3c(pro) of fmdv are involved in the inhibition of type i ifn production. however, whether the proteins of fmdv can inhibit type i ifn signaling is less well understood. in this stud ...	2014	24554650
picornavirus infection leading to immunosuppression.	viruses, such as hiv, hepatitis a, poliovirus, coxsackievirus b3 and foot-and-mouth disease virus, use a variety of mechanisms to suppress the human immune system in order to evade clearance by the host. therefore, investigating how a few changes in the viral genome of a nonlethal virus can lead to an alteration in disease, from survivable to immunosuppression and death, would provide valuable information into viral pathogenesis. in addition, we propose that gaining a better insight into how the ...	2014	25214881
cellular micrornas and picornaviral infections.	micrornas (mirnas) are a subtype of short, endogenous, and non-coding rnas, which post-transcriptionally regulate gene expression. the mirna-mediated gene silencing mechanism is involved in a wide spectrum of biological processes, such as cellular proliferation, differentiation, and immune responses. picornaviridae is a large family of rna viruses, which includes a number of causative agents of many human and animal diseases viz., poliovirus, foot-and-mouth disease virus (fmdv), and coxsackievir ...	2014	24921242
potential applications for antiviral therapy and prophylaxis in bovine medicine.	viral disease is one of the major causes of financial loss and animal suffering in today's cattle industry. increases in global commerce and average herd size, urbanization, vertical integration within the industry and alterations in global climate patterns have allowed the spread of pathogenic viruses, or the introduction of new viral species, into regions previously free of such pathogens, creating the potential for widespread morbidity and mortality in naïve cattle populations. despite this, ...	2014	24810855
pathogens in water deer (hydropotes inermis) in south korea, 2010-12.	water deer (hydropotes inermis) are among the most common wildlife to approach farmhouses and livestock barns in korea. we collected 305 water deer from gangwon (n=168), south chungcheong (n=89), and gyeongsang (n=48) provinces in 2010-12 and used pcr and serologic tests to screen the deer for pathogens. in 2010, tests for bovine viral diarrhea virus (bvdv), rotavirus, and brucella abortus were positive in 8% (5/60), 2% (1/60), and 59% (33/56) of the animals, respectively. in 2010, the water dee ...	2014	24779466
development of loop-mediated isothermal amplification assay for specific and rapid detection of differential goat pox virus and sheep pox virus.	capripox viruses are economically important pathogens in goat and sheep producing areas of the world, with specific focus on goat pox virus (gtpv), sheep pox virus (sppv) and the lumpy skin disease virus (lsdv). clinically, sheep pox and goat pox have the same symptoms and cannot be distinguished serologically. this presents a real need for a rapid, inexpensive, and easy to operate and maintain genotyping tool to facilitate accurate disease diagnosis and surveillance for better management of cap ...	2014	24438089
in vitro surrogate models to aid in the development of antivirals for the containment of foot-and-mouth disease outbreaks.	foot-and-mouth disease virus (fmdv) is a highly pathogenic member of the genus aphthovirus (family picornaviridae) that is only to be manipulated in high-containment facilities, thus complicating research on and discovery of antiviral strategies against the virus. bovine rhinitis b virus (brbv) and equine rhinitis a virus (erav), phylogenetically most closely related to fmdv, were explored as surrogates for fmdv in antiviral studies. although no efficient cell culture system has been reported so ...	2014	24583031
adjuvant effects of l. acidophilus lw1 on immune responses to the foot-and-mouth disease virus dna vaccine in mice.	the adjuvant effects of lactobacillus acidophilus on dna vaccination are not fully understood. it has been hypothesized that swine-derived lactobacillus acidophilus sw1 (lasw1) could function as an immune adjuvant to enhance antigen-specific immune responses after foot-and-mouth disease (fmd) dna vaccination in mice. to evaluate the effect of oral lasw1 on the immune response to a dna vaccine (prc/cmv-vp1) harboring fmd vp1 gene, anti-fmdv antibody and its isotypes, t-cell proliferation, and cyt ...	2014	25119375
construction of self-replicating subgenomic dengue virus 4 (denv4) replicon.	dengue virus serotypes 1-4 are members of mosquito-borne flavivirus genus of flaviviridae family that encode one long open reading frame (orf) that is translated to a polyprotein. both host and virally encoded proteases function in the processing of the polyprotein by co-translational and posttranslational mechanisms to yield 10 mature proteins prior to viral rna replication. to study cis- and trans-acting factors involved in viral rna replication, many groups [1-8] have constructed cdnas encodi ...	2014	24696335
rna structure disrupting g320-t transversion within the short fragment of the 5' untranslated region prevents rescue of infectious foot-and-mouth disease virus.	a full-length cdna clone of an indian foot-and-mouth disease virus strain, asia 1 ind 491/1997 was assembled downstream of the t7 promoter in the pbluescript ii sk (+) vector by sequential ligation of four pcr-generated subgenomic fragments. rna transcribed from that construct were transfected into bhk-21 and lfbk cells to rescue infectious virus. the in vitro growth kinetics, plaque morphology, infectivity titer, antigenic profile and virulence characteristics in unweaned mice infected with the ...	2014	24269793
induction and suppression of innate antiviral responses by picornaviruses.	the family picornaviridae comprises of small, non-enveloped, positive-strand rna viruses and contains many human and animal pathogens including enteroviruses (e.g. poliovirus, coxsackievirus, enterovirus 71 and rhinovirus), cardioviruses (e.g. encephalomyocarditis virus), hepatitis a virus and foot-and-mouth disease virus. picornavirus infections activate a cytosolic rna sensor, mda5, which in turn, induces a type i interferon response, a crucial component of antiviral immunity. moreover, picorn ...	2014	25086453
modification of picornavirus genomic rna using 'click' chemistry shows that unlinking of the vpg peptide is dispensable for translation and replication of the incoming viral rna.	picornaviruses constitute a large group of viruses comprising medically and economically important pathogens such as poliovirus, coxsackievirus, rhinovirus, enterovirus 71 and foot-and-mouth disease virus. a unique characteristic of these viruses is the use of a viral peptide (vpg) as primer for viral rna synthesis. as a consequence, all newly formed viral rna molecules possess a covalently linked vpg peptide. it is known that vpg is enzymatically released from the incoming viral rna by a host p ...	2014	24243841
all-atom molecular dynamics calculation study of entire poliovirus empty capsids in solution.	small viruses that belong, for example, to the picornaviridae, such as poliovirus and foot-and-mouth disease virus, consist simply of capsid proteins and a single-stranded rna (ssrna) genome. the capsids are quite stable in solution to protect the genome from the environment. here, based on long-time and large-scale 6.5 × 10(6) all-atom molecular dynamics calculations for the mahoney strain of poliovirus, we show microscopic properties of the viral capsids at a molecular level. first, we found e ...	2014	25362342
l protease from foot and mouth disease virus confers eif2-independent translation for mrnas bearing picornavirus ires.	the leader protease (l(pro)) from foot-and-mouth disease virus (fmdv) has the ability to cleave eif4g, leading to a blockade of cellular protein synthesis. in contrast to previous reports, our present findings demonstrate that fmdv l(pro) is able to increase translation driven by fmdv ires. additionally, inactivation of eif2 subsequent to phosphorylation induced by arsenite or thapsigargin in bhk cells blocks protein synthesis directed by fmdv ires, whereas in the presence of l(pro), significant ...	2014	25268112
capsid protein vp4 of human rhinovirus induces membrane permeability by the formation of a size-selective multimeric pore.	non-enveloped viruses must deliver their viral genome across a cell membrane without the advantage of membrane fusion. the mechanisms used to achieve this remain poorly understood. human rhinovirus, a frequent cause of the common cold, is a non-enveloped virus of the picornavirus family, which includes other significant pathogens such as poliovirus and foot-and-mouth disease virus. during picornavirus cell entry, the small myristoylated capsid protein vp4 is released from the virus, interacts wi ...	2014	25102288
ribavirin-resistant variants of foot-and-mouth disease virus: the effect of restricted quasispecies diversity on viral virulence.	mutagenic nucleoside analogues can be used to isolate rna virus high-fidelity rna-dependent rna polymerase (rdrp) variants, the majority of which are attenuated in vivo. however, attenuated foot-and-mouth disease virus (fmdv) high-fidelity rdrp variants have not been isolated, and the correlations between rdrp fidelity and virulence remain unclear. here, the mutagen ribavirin was used to select a ribavirin-resistant population of fmdv, and 4 amino acid substitutions (d5n, a38v, m194i, and m296v) ...	2014	24453363
strategies for purifying variants of human rhinovirus 14 2c protein.	the positive strand rna genome of picornaviruses, including human rhinovirus (hrv), poliovirus (pv) and foot-and-mouth disease virus, is translated immediately into a polyprotein that is cleaved by virally encoded proteinases into 10-13 mature proteins. these include the four proteins required to assemble the viral particle as well as 3d(pol) (the viral rna polymerase) and 2c, an atpase and putative helicase. 2c is a protein which is responsible, together with 2b and 3a, for anchoring the replic ...	2014	24316192
synonymous deoptimization of foot-and-mouth disease virus causes attenuation in vivo while inducing a strong neutralizing antibody response.	codon bias deoptimization has been previously used to successfully attenuate human pathogens, including poliovirus, respiratory syncytial virus, and influenza virus. we have applied a similar technology to deoptimize the capsid-coding region (p1) of foot-and-mouth disease virus (fmdv). despite the introduction of 489 nucleotide changes (19%), synonymous deoptimization of the p1 region rendered a viable fmdv progeny. the resulting strain was stable and reached cell culture titers similar to those ...	2015	26581977
cleavage efficient 2a peptides for high level monoclonal antibody expression in cho cells.	linking the heavy chain (hc) and light chain (lc) genes required for monoclonal antibodies (mab) production on a single cassette using 2a peptides allows control of lc and hc ratio and reduces non-expressing cells. four 2a peptides derived from the foot-and-mouth disease virus (f2a), equine rhinitis a virus (e2a), porcine teschovirus-1 (p2a) and thosea asigna virus (t2a), respectively, were compared for expression of 3 biosimilar igg1 mabs in chinese hamster ovary (cho) cell lines. hc and lc wer ...	2015	25621616
promotion of viral ires-mediated translation initiation under mild hypothermia.	internal ribosome entry site (ires)-mediated translation is an essential replication step for certain viruses. as ires-mediated translation is regulated differently from cap-dependent translation under various cellular conditions, we sought to investigate whether temperature influences efficiency of viral ires-mediated translation initiation by using bicistronic reporter constructs containing an ires element of encephalomyocarditis virus (emcv), foot-and-mouth disease virus (fmdv), hepatitis c v ...	2015	25951166
mgmt enrichment and second gene co-expression in hematopoietic progenitor cells using separate or dual-gene lentiviral vectors.	the dna repair gene o(6)-methylguanine-dna methyltransferase (mgmt) allows efficient in vivo enrichment of transduced hematopoietic stem cells (hsc). thus, linking this selection strategy to therapeutic gene expression offers the potential to reconstitute diseased hematopoietic tissue with gene-corrected cells. however, different dual-gene expression vector strategies are limited by poor expression of one or both transgenes. to evaluate different co-expression strategies in the context of mgmt-m ...	2015	25479595
both foot-and-mouth disease virus and bovine viral diarrhea virus replication are inhibited by mx1 protein originated from porcine.	mx1 protein is i type interferons (ifns)-induced 76-kda guanosine triphosphatases (gtpases) that belong to the dynamin superfamily of large gtpases. mx1 proteins have attracted attention because some display antiviral activity against pathogenic rna and dna viruses. meanwhile, mx1 gene generally exists in organisms or cells of mammalian, fish and chicken. blocking a wide range of rna virus replication by inhibiting nuclear viral mrna synthesis is a unique property of mx1 protein. in order to inv ...	2015	25153459
structural analysis and immunogenicity of recombinant major envelope protein (ra27l) of buffalopox virus, a zoonotic indian vaccinia-like virus.	buffalopox virus (bpxv), an indian variant of vaccinia virus (vacv), is a zoonotic agent and affects buffaloes, cattle and humans. a27l is one of the conserved major immuno-dominant envelope proteins of orthopox viruses (opvs) involved in viral entry/maturation and elicits neutralizing antibodies. in this study, the a27l gene of bpxv-vij/96 strain encoding recombinant mature a27l (21s to e110) and c-terminal truncated a27l-lzd (21s to n84aa) proteins were cloned and over-expressed in escherichia ...	2015	26319070
plant-produced viral bovine vaccines: what happened during the last 10 years?	vaccination has proved to be an efficient strategy to deal with viral infections in both human and animal species. however, protection of cattle against viral infections is still a major concern in veterinary science. during the last two decades, the development of efficient plant-based expression strategies for recombinant proteins prompted the application of this methodology for veterinary vaccine purposes. the main goals of viral bovine vaccines are to improve the health and welfare of cattle ...	2015	26250843
simultaneous detection of five notifiable viral diseases of cattle by single-tube multiplex real-time rt-pcr.	multiplexed real-time pcr (qpcr) assays enable the detection of several target genes in a single reaction, which is applicable for simultaneous testing for the most important viral diseases in samples obtained from ruminants with unspecific clinical symptoms. here, reverse transcription qpcr (rt-qpcr) systems for the detection of bovine viral diarrhoea virus (bvdv) and bluetongue virus (btv) were combined with an internal control system based on the beta-actin gene. additionally, a background sc ...	2015	25746154
prioritisation of wildlife pathogens to be targeted in european surveillance programmes: expert-based risk analysis focus on ruminants.	this study attempted to develop a list of priority pathogens. it is part of a european union (eu) project dedicated to the surveillance of emerging or re-emerging pathogens of wildlife. partners of the consortium established an initial list of 138 pathogens of concern, which was reduced to a smaller list of 65 pathogens likely to affect ruminants (i.e., the most costly animal group in the eu over the last 15 years). these 65 pathogens underwent a two-step, expert-based risk analysis: 92 experts ...	2015	25496774
occurrence of antibodies anti -toxoplasma gondii, neospora caninum and leptospira interrogans in a captive deer herd in southern brazil.	a large number of brazilian zoos keep many endangered species of deer, however, very few disease surveillance studies have been conducted among captive cervids. blood samples from 32 brazilian deer (blastocerus dichotomus, mazama nana and mazama americana) kept in captivity at bela vista biological sanctuary (foz do iguaçu, brazil) were investigated for 10 ruminant pathogens, with the aims of monitoring deer health status and evaluating any potential zoonotic risk. deer serum samples were tested ...	2015	26689185
nmr analysis of the interaction of picornaviral proteinases lb and 2a with their substrate eukaryotic initiation factor 4gii.	messenger rna is recruited to the eukaryotic ribosome by a complex including the eukaryotic initiation factor (eif) 4e (the cap-binding protein), the scaffold protein eif4g and the rna helicase eif4a. to shut-off host-cell protein synthesis, eif4g is cleaved during picornaviral infection by a virally encoded proteinase; the structural basis of this reaction and its stimulation by eif4e is unclear. we have structurally and biochemically investigated the interaction of purified foot-and-mouth dise ...	2015	26384734
recovery of infectious type asia1 foot-and-mouth disease virus from suckling mice directly inoculated with an rna polymerase i/ii-driven unidirectional transcription plasmid.	we developed an rna polymerase (pol) i- and ii-driven plasmid-based reverse genetics system to rescue infectious foot-and-mouth disease virus (fmdv) from cloned cdna. in this plasmid-based transfection, the full-length viral cdna was flanked by hammerhead ribozyme (hamrz) and hepatitis delta ribozyme (hdvrz) sequences, which were arranged downstream of the two promoters (cytomegalovirus (cmv) and pol i promoter) and upstream of the terminators and polyadenylation signal, respectively. the utilit ...	2015	26091821
construction and characterization of recombinant human adenovirus type 5 expressing foot-and-mouth disease virus capsid proteins of indian vaccine strain, o/ind/r2/75.	generation of recombinant human adenovirus type 5 expressing foot-and-mouth disease virus (fmdv) capsid protein genes along with full-length 2b, 3b and 3c(pro) and its characterization.	2015	27047064
robust real-time reverse transcription-pcr for detection of foot-and-mouth disease virus neutralizing carryover contamination.	during an outbreak of foot-and-mouth disease (fmd), real-time reverse transcription-pcr (rrt-pcr) is the most commonly used diagnostic method to detect viral rna. however, while this assay is often conducted during the outbreak period, there is an inevitable risk of carryover contamination. this study shows that the carryover contamination can be prevented by the use of target-specific restriction endonuclease in that assay.	2015	26560537
feasibility of cowpea chlorotic mottle virus-like particles as scaffold for epitope presentations.	within the last decade virus-like particles (vlps) have increasingly received attention from scientists for their use as a carrier of (peptide) molecules or as scaffold to present epitopes for use in subunit vaccines. to test the feasibility of cowpea chlorotic mottle virus (ccmv) particles as a scaffold for epitope presentation and identify sites for epitope fusion or insertion that would not interfere with virus-like-particle formation, chimeric ccmv coat protein (cp) gene constructs were engi ...	2015	26311254
detection of african swine fever, classical swine fever, and foot-and-mouth disease viruses in swine oral fluids by multiplex reverse transcription real-time polymerase chain reaction.	african swine fever (asf), classical swine fever (csf), and foot-and-mouth disease (fmd) are highly contagious animal diseases of significant economic importance. pigs infected with asf and csf viruses (asfv and csfv) develop clinical signs that may be indistinguishable from other diseases. likewise, various causes of vesicular disease can mimic clinical signs caused by the fmd virus (fmdv). early detection is critical to limiting the impact and spread of these disease outbreaks, and the ability ...	2015	25776540
bioinformatics and molecular analysis of the evolutionary relationship between bovine rhinitis a viruses and foot-and-mouth disease virus.	bovine rhinitis viruses (brvs) cause mild respiratory disease of cattle. in this study, a near full-length genome sequence of a virus named rs3x (formerly classified as bovine rhinovirus type 1), isolated from infected cattle from the uk in the 1960s, was obtained and analyzed. compared to other closely related aphthoviruses, major differences were detected in the leader protease (l(pro)), p1, 2b, and 3a proteins. phylogenetic analysis revealed that rs3x was a member of the species bovine rhinit ...	2015	27081310
synthetic rnas mimicking structural domains in the foot-and-mouth disease virus genome elicit a broad innate immune response in porcine cells triggered by rig-i and tlr activation.	the innate immune system is the first line of defense against viral infections. exploiting innate responses for antiviral, therapeutic and vaccine adjuvation strategies is being extensively explored. we have previously described, the ability of small in vitro rna transcripts, mimicking the sequence and structure of different domains in the non-coding regions of the foot-and-mouth disease virus (fmdv) genome (ncrnas), to trigger a potent and rapid innate immune response. these synthetic non-infec ...	2015	26193305
encephalomyocarditis virus 3c protease relieves traf family member-associated nf-κb activator (tank) inhibitory effect on traf6-mediated nf-κb signaling through cleavage of tank.	traf family member-associated nf-κb activator (tank) is a negative regulator of canonical nf-κb signaling in the toll-like receptor- and b-cell receptor-mediated signaling pathways. however, functions of tank in viral infection-mediated nf-κb activation remain unclear. here, we reported that tank was cleaved by encephalomyocarditis virus 3c at the 197 and 291 glutamine residues, which depends on its cysteine protease activity. in addition, encephalomyocarditis virus 3c impaired the ability of ta ...	2015	26363073
evaluation of a fiber-modified adenovirus vector vaccine against foot-and-mouth disease in cattle.	novel vaccination approaches against foot-and-mouth disease (fmd) include the use of replication-defective human adenovirus type 5 (ad5) vectors that contain the capsid-encoding regions of fmd virus (fmdv). ad5 containing serotype a24 capsid sequences (ad5.a24) has proved to be effective as a vaccine against fmd in livestock species. however, ad5-vectored fmdv serotype o1 campos vaccine (ad5.o1c.2b) provides only partial protection of cattle against homologous challenge. it has been reported tha ...	2015	26607309
heterozygosity at the snp (rs136500299) of itgb6 receptor gene possibly influences the susceptibility among crossbred bull to foot and mouth disease infection.	zebu (bos indicus) cattle are known to be resistant against foot and mouth disease virus (fmdv) compared to taurine (bos taurus). to understand the susceptibility of two cattle species to fmdv infection in terms of viral receptors, the present study reports the cloning, characterization and sequence analysis of zebu itgb6 gene. the complete cds of zebu itgb6 was 2367 basepair in length with 788 amino acid residues. the zebu integrin shares common structural and functional elements with taurine a ...	2015	26436121
antigenic and genetic comparison of foot-and-mouth disease virus serotype o indian vaccine strain, o/ind/r2/75 against currently circulating viruses.	foot-and-mouth disease (fmd) virus serotype o is the most common cause of fmd outbreaks in india and three of the six lineages that have been described are most frequently detected, namely ind2001, panasia and panasia 2. we report the full capsid sequence of 21 serotype o viruses isolated from india between 2002 and 2012. all these viruses belong to the middle east-south asia (me-sa) topotype. the serological cross-reactivity of a bovine post-vaccination serum pool raised against the current ind ...	2015	25500306
foot-and-mouth disease vaccination induces cross-reactive ifn-γ responses in cattle that are dependent on the integrity of the 140s particles.	interferon-γ (ifn-γ) recall responses against foot-and-mouth disease virus (fmdv) in fmd vaccinated cattle are utilized to study t-lymphocyte immunity against this virus. here, a recall ifn-γ assay based on a commercial elisa was set up using 308 samples from naïve and vaccinated cattle. the assay was used to study cross-reactive responses between different fmdv vaccine strains. blood samples from cattle immunized with monovalent vaccines containing a24/cruzeiro/brazil/55, a/argentina/2001 or o1 ...	2015	25496826
megaprimer-mediated capsid swapping for the construction of custom-engineered chimeric foot-and-mouth disease virus.	foot-and-mouth disease (fmd) is a highly contagious, economically important disease of transboundary importance. regular vaccination with chemically inactivated fmd vaccine is the major means of controlling the disease in endemic countries like india. however, the selection of appropriate candidate vaccine strain and its adaptation in cell culture to yield high titer of virus is a cumbersome process. an attractive approach to circumvent this tedious process is to replace the capsid coding sequen ...	2015	26303897
synthesis and in-vitro evaluation of 2-amino-4-arylthiazole as inhibitor of 3d polymerase against foot-and-mouth disease (fmd).	foot-and-mouth disease (fmd) is a highly contagious vesicular disease of livestock caused by a highly variable rna virus, foot-and-mouth disease virus (fmdv). one of the targets to suppress expansion of and to control fmd is 3d polymerase (fmdv 3dpol). in this study, 2-amino-4-arylthiazole derivatives were synthesized and evaluated for their inhibitory activity against fmdv 3dpol. among them, compound 20i exhibited the most potent functional inhibition (ic50 = 0.39 μm) of fmdv 3d polymerase and ...	2015	26301555
early protection in sheep against intratypic heterologous challenge with serotype o foot-and-mouth disease virus using high-potency, emergency vaccine.	in 2009-2011, spread of a serotype o foot-and-mouth disease virus (fmdv) belonging to the south east asia topotype led to the culling of over 3.5 million cattle and pigs in japan and korea. the o1 manisa vaccine (belonging to the middle east-south asian topotype) was used at high potency in korea to limit the expansion of the outbreak. however, no data are available on the spread of this virus or the efficacy of the o1 manisa vaccine against this virus in sheep. in this study, the early protecti ...	2015	25483241
vaccination of cattle only is sufficient to stop fmdv transmission in mixed populations of sheep and cattle.	we quantified the transmission of foot-and-mouth disease virus in mixed cattle-sheep populations and the effect of different vaccination strategies. the (partial) reproduction ratios (r) in groups of non-vaccinated and vaccinated cattle and/or sheep were estimated from (published) transmission experiments. a 4 × 4 next-generation matrix (ngm) was constructed using these estimates. the dominant eigenvalue of the ngm, the r for a mixed population, was determined for populations with different prop ...	2015	25464822
multifunctionality of a picornavirus polymerase domain: nuclear localization signal and nucleotide recognition.	the n-terminal region of the foot-and-mouth disease virus (fmdv) 3d polymerase contains the sequence mrktklapt (residues 16 to 24) that acts as a nuclear localization signal. a previous study showed that substitutions k18e and k20e diminished the transport to the nucleus of 3d and 3cd and severely impaired virus infectivity. these residues have also been implicated in template binding, as seen in the crystal structures of different 3d-rna elongation complexes. here, we report the biochemical and ...	2015	25903341
serotyping of foot and mouth disease virus and pasteurella multocida from indian gaurs (bos gaurus), concurrently infected with foot and mouth disease and haemorrhagic septicaemia.	we report the serotyping of foot-and-mouth disease virus (fmdv) and pasteurella multocida from indian gaurs which were concurrently infected with foot-and-mouth disease (fmd) and haemorrhagic septicaemia. bannerghatta biological park (bbp), a national park located in the outskirts of bengaluru city, karnataka, india, is bordered by several villages. these villages witnessed massive outbreaks of fmd which spread rapidly to the herbivores at bbp. post-mortem was conducted on carcasses of two india ...	2015	25894817
impact of foot-and-mouth disease on mastitis and culling on a large-scale dairy farm in kenya.	foot and mouth disease (fmd) is a highly transmissible viral infection of cloven hooved animals associated with severe economic losses when introduced into fmd-free countries. information on the impact of the disease in fmdv-endemic countries is poorly characterised yet essential for the prioritisation of scarce resources for disease control programmes. a fmd (virus serotype sat2) outbreak on a large-scale dairy farm in nakuru county, kenya provided an opportunity to evaluate the impact of fmd o ...	2015	25889460
development of a non-infectious encapsidated positive control rna for molecular assays to detect foot-and-mouth disease virus.	positive controls are an important component of the quality-control of molecular tests used for diagnosis of livestock diseases. for high consequence agents such as foot-and-mouth disease virus (fmdv), the positive controls required to monitor template extraction, reverse transcription and amplification steps usually consist of material derived from infectious viruses. therefore, their production is dependent upon the use of high containment facilities and their deployment carries the risks asso ...	2015	25864934
the impact of within-herd genetic variation upon inferred transmission trees for foot-and-mouth disease virus.	full-genome sequences have been used to monitor the fine-scale dynamics of epidemics caused by rna viruses. however, the ability of this approach to confidently reconstruct transmission trees is limited by the knowledge of the genetic diversity of viruses that exist within different epidemiological units. in order to address this question, this study investigated the variability of 45 foot-and-mouth disease virus (fmdv) genome sequences (from 33 animals) that were collected during 2007 from eigh ...	2015	25861750
an appraisal on the occurrence of foot-and-mouth disease virus serotypes in cattle and buffaloes, pakistan.	foot-and-mouth disease (fmd) is a livestock disease that has serious economic repercussions. here, we report the laboratory results for samples from suspected outbreaks that were sent for confirmation of fmd in pakistan. from january 2010 to december 2011, 132 suspected outbreaks were investigated, and samples from 58 out of the 76 outbreaks sent to the national veterinary laboratory (nvl) were positive. the highest proportion of positives were of serotype o (65.52 %), followed by serotype a (24 ...	2015	25850759
stabilizing effects of excipients on dissociation of intact (146s) foot-and-mouth disease virions into 12s particles during storage as oil-emulsion vaccine.	most conventional foot-and-mouth disease virus (fmdv) vaccines contain oil-adjuvant. their potency decreases upon prolonged storage. intact (146s) fmdv particles can dissociate into 12s degradation products with a concomitant decrease in immunogenicity. we therefore measured virion stability in vaccines using two previously developed elisas to separately quantify 12s and 146s particles. virions completely dissociated into 12s particles within 3 months after oil-emulsification. dissociation occur ...	2015	25843267
phylogenetic analysis of the leader proteinase (lpro) region of indian foot and mouth disease serotype o isolates.	in this study, the nucleotide sequences of the complete leader proteinase (lpro) region of 21 isolates of foot-and-mouth disease virus (fmdv) serotype o collected during various outbreaks in india were sequenced and compared with vaccine strains. the phylogenetic analysis of these lpro sequences showed a difference in the clustering of the isolates based on the vp1 capsid coding region sequences. the comparison of amino acid sequences at the n terminus end of the lpro region showed very high var ...	2015	25842211
risk factors for foot and mouth disease outbreaks in grazing beef cattle herds.	foot and mouth disease (fmd) is considered one of the most important diseases of cattle. recurrence of fmd outbreaks in israel is common, even though routine vaccination of livestock is mandatory and control measures are applied during the outbreaks. grazing beef herds are occasionally involved in these outbreaks and play an important role in disseminating the disease, due to the large efflux of animals from these herds to feedlots. nevertheless, the risk factors for the occurrence of fmd among ...	2015	25841998
apigenin restricts fmdv infection and inhibits viral ires driven translational activity.	foot-and-mouth disease (fmd) is a highly contagious disease of domestic and wild ruminants that is caused by fmd virus (fmdv). fmd outbreaks have occurred in livestock-containing regions worldwide. apigenin, which is a flavonoid naturally existing in plant, possesses various pharmacological effects, including anti-inflammatory, anticancer, antioxidant and antiviral activities. results show that apigenin can inhibit fmdv-mediated cytopathogenic effect and fmdv replication in vitro. further studie ...	2015	25835532
determination of common genetic variants within the non-structural proteins of foot-and-mouth disease viruses isolated in sub-saharan africa.	the non-structural proteins of foot-and-mouth disease virus (fmdv) are responsible for rna replication, proteolytic processing of the viral polyprotein precursor, folding and assembly of the structural proteins and modification of the cellular translation apparatus. investigation of the amino acid heterogeneity of the non-structural proteins of seventy-nine fmdv isolates of sat1, sat2, sat3, a and o serotypes revealed between 29 and 62% amino acid variability. the leader protease (l(pro)) and 3a ...	2015	25818579
a computational study of the interaction of the foot and mouth disease virus vp1 with monoclonal antibodies.	foot and mouth disease is caused by a non-enveloped virus (fmdv), which disposes several antigenic sites at the surface of their capsid proteins. the most relevant and immunodominant antigenic site of fmdv (site a or ansa) includes a key virus-cell interaction element (rgd motif) located in the viral protein 1 (vp1), more precisely at the gh loop. ansa includes a set of overlapped and mainly linear epitopes, which are the main targets of the humoral immune response. taking advantage over specifi ...	2015	26093030
transgenic shrna pigs reduce susceptibility to foot and mouth disease virus infection.	foot-and-mouth disease virus (fmdv) is an economically devastating viral disease leading to a substantial loss to the swine industry worldwide. a novel alternative strategy is to develop pigs that are genetically resistant to infection. here, we produce transgenic (tg) pigs that constitutively expressed fmdv-specific short interfering rna (sirna) derived from small hairpin rna (shrna). in vitro challenge of tg fibroblasts showed the shrna suppressed viral growth. tg and non-tg pigs were challeng ...	2015	26090904
comparative performance of fetal goat tongue cell line zz-r 127 and fetal porcine kidney cell line lfbk-αvβ6 for foot-and-mouth disease virus isolation.	the fetal goat tongue cell line zz-r 127 and the fetal porcine kidney cell line lfbk-α(v)β(6) have been reported to have high sensitivity to various foot-and-mouth disease virus (fmdv) strains. the suitability of zz-r 127 cells for fmdv isolation not only from epithelial suspensions but also from other clinical samples has already been confirmed in a previous study. however, to our knowledge, the suitability of lfbk-α(v)β(6) cells has not been evaluated using clinical samples other than epitheli ...	2015	26082457
sat2 foot and mouth disease (fmd) outbreak in a mixed farm in egypt.	a dairy farm keeping holstein cattle and buffaloes in the menoufia governorate was investigated during and after the last foot and mouth disease (fmd) outbreak in egypt (starting february 2012) to determine the impact of the outbreak on animals as well as to assess some factors that might have helped to spread the disease in the investigated farm. all animals were vaccinated against fmd with the locally produced bivalent vaccine containing o1 and a/egy/2006 strains two months before the onset of ...	2015	26054223
evolutionary dynamics of foot-and-mouth disease virus o/me-sa/ind2001 lineage.	foot-and-mouth disease (fmd) virus serotype o ind2001 lineage within the middle east-south asia topotype is the major cause of recent fmd incidences in india. a sub-lineage of ind2001 caused severe outbreaks in the southern region of the country during 2013 and also reported for the first time from libya. in this study, we conducted a detailed evolutionary analysis of ind2001 lineage. phylogenetic analysis of ind2001 lineage based on maximum likelihood method revealed two major splits and three ...	2015	26049591
expression of porcine mx1 with fmdv ires enhances the antiviral activity against foot-and-mouth disease virus in pk-15 cells.	foot-and-mouth disease virus (fmdv) is the most contagious pathogen in cloven-hoofed (two-toed) animals. due to the rapid replication and spread of fmdv, novel therapeutic strategies are greatly needed to reduce or block fmdv shedding in cases of disease outbreak. here, we generated an ires-mx1 construct in which the internal ribosome entry site (ires) of fmdv was inserted between the promoter and open reading frame (orf) of porcine myxovirus resistance protein 1 (pomx1). this construct provides ...	2015	26047650
robust protection against highly virulent foot-and-mouth disease virus in swine by combination treatment with recombinant adenoviruses expressing porcine alpha and gamma interferons and multiple small interfering rnas.	because the currently available vaccines against foot-and-mouth disease (fmd) provide no protection until 4 to 7 days postvaccination, the only alternative method to halt the spread of the fmd virus (fmdv) during outbreaks is the application of antiviral agents. combination treatment strategies have been used to enhance the efficacy of antiviral agents, and such strategies may be advantageous in overcoming viral mechanisms of resistance to antiviral treatments. we have developed recombinant aden ...	2015	26041279
[construction and characterization of an epitope-mutated asia 1 type foot-and-mouth disease virus].	to generate an epitope-mutated foot-and-mouth disease virus (fmdv) as a marker vaccine, the infectious clone pasia 1-fmdv containing the complete genomic cdna of asia 1 type fmdv was used as backbone, the residues at positions 27 and 31 in the 3d gene were mutated (h27y and n31r). the resulting plasmid pasia 1-fmdv-3dm encoding a mutated epitope was transfected into bhk-21 cells and the recombinant virus rasia 1-3dm was rescued. the recombinant virus showed similar biological characteristics com ...	2015	26021083
capsid coding region diversity of re-emerging lineage c foot-and-mouth disease virus serotype asia1 from india.	foot-and-mouth disease virus (fmdv) serotype asia1 was first reported in india in 1951, where three major genetic lineages (b, c and d) of this serotype have been described until now. in this study, the capsid protein coding region of serotype asia1 viruses (n = 99) from india were analyzed, giving importance to the viruses circulating since 2007. all of the isolates (n = 50) recovered during 2007-2013 were found to group within the re-emerging cluster of lineage c (designated as sublineage c(r) ...	2015	26008211
persistent foot-and-mouth disease virus infection in the nasopharynx of cattle; tissue-specific distribution and local cytokine expression.	tissues obtained post-mortem from cattle persistently infected with foot-and-mouth disease virus (fmdv) were analyzed to characterize the tissue-specific localization of fmdv and partial transcriptome profiles for selected immunoregulatory cytokines. analysis of 28 distinct anatomic sites from 21 steers infected with fmdv serotype a, o or sat2, had the highest prevalence of overall viral detection in the dorsal nasopharynx (80.95%) and dorsal soft palate (71.43%). fmdv was less frequently detect ...	2015	25996935
different functional sensitivity to mutation at intersubunit interfaces involved in consecutive stages of foot-and-mouth disease virus assembly.	small spherical viruses are paradigms of supramolecular self-assembly. identifying the specific structural determinants for virus assembly provides guidelines to develop new antiviral drugs or engineer modified viral particles for medical or technological applications. however, very few systematic studies have been carried out so far to identify those chemical groups at interfaces between virus capsid subunits that are important for viral assembly and function. foot-and-mouth disease virus (fmdv ...	2015	25979732
development of a multiplex lateral flow strip test for foot-and-mouth disease virus detection using monoclonal antibodies.	foot-and-mouth disease (fmd) is one of the world's most highly contagious animal diseases with tremendous economic consequences. a rapid and specific test for fmd diagnosis at the site of a suspected outbreak is crucial for the implementation of control measures. this project developed a multiplex lateral flow immunochromatographic strip test (multiplex-lfi) for the rapid detection and serotyping of fmd viruses. the monoclonal antibodies (mabs) against serotypes o, a, and asia 1 were used as cap ...	2015	25977185