Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| identification of epstein-barr nuclear antigen polypeptide in mouse and monkey cells after gene transfer with a cloned 2.9-kilobase-pair subfragment of the genome. | a new antigenic polypeptide was identified in mouse l cells and in monkey cos-1 cells in which epstein-barr nuclear antigen (ebna) was expressed as the result of gene transfer with cloned fragments of epstein-barr virus (ebv) dna. the same-size protein (mr, approximately equal to 78,000) was seen in stably transformed mouse cells harboring only the bamhi k fragment [approximately equal to 5.2 kilobase pairs (kbp)] or its bamhi/hindiii subfragment, i1f (approximately equal to 2.9 kbp). thus, the ... | 1984 | 6320171 |
| effect of (e)-5-(2-bromovinyl)-2'-deoxyuridine on several parameters of epstein-barr virus infection. | the selective and potent anti-herpesvirus drug, (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu), has been examined for its inhibitory effects on several parameters of epstein-barr virus (ebv) infection in the lymphoblastoid cell lines raji, p3hr-1, b-95-8 and p3 hybrid cells (a human embryo oropharyngeal cell line fused with a nasopharyngeal carcinoma cell line). at a dosage of 0.03 to 0.1 mm, bvdu caused a marked inhibition of (i) spontaneous viral capsid antigen (vca) expression in b-95-8 and p3 h ... | 1984 | 6319572 |
| a structural investigation of the epstein-barr (eb) virus membrane antigen glycoprotein, gp340. | epstein-barr (eb) virus membrane antigen (ma) glycoprotein (gp340) purified by a molecular weight-based technique has been subjected to biochemical analysis. following treatment with glycosidases or tunicamycin during synthesis, the carbohydrate moiety was found to be made up of both o-linked and n-linked types and to constitute about 50% of the molecular mass. digestion studies with neuraminidase and oligosaccharidase have indicated that the molecule is heavily sialated with most of the sialic ... | 1984 | 6319581 |
| an epstein-barr virus dna fragment encodes messages for the two major envelope glycoproteins (gp350/300 and gp220/200). | the genes encoding the two major epstein-barr virus glycoproteins (gp350/300 and gp220/200) have been mapped to a 5-kilobase fragment of the viral genome (bamhi-l). this fragment encodes 3.4- and 2.8-kilobase rnas which translate proteins of 135 and 100 kilodaltons, respectively. both proteins react with antiserum specific for gp350/300 and gp220/200. the 135-kilodalton protein is identical in size to the nascent polypeptide precursor to gp350/300, and the 100-kilodalton protein is the expected ... | 1984 | 6319741 |
| epstein-barr virus-specific dnase activity in nonproducer raji cells after treatment with 12-o-tetradecanoylphorbol-13-acetate and sodium butyrate. | an epstein-barr virus (ebv)-specific dnase was induced in ebv nonproducer raji cells after treatment with 12-o-tetradecanoylphorbol-13-acetate and sodium butyrate. the increase in ebv dnase activity was related to the appearance of early antigen-positive cells. the enzyme had a sedimentation coefficient of 4s and was resistant to 300 mm kcl, and its induction did not depend on viral dna synthesis. the ebv-specific dnase activity was specifically inhibited by sera from patients who had nasopharyn ... | 1984 | 6319766 |
| epstein-barr virus susceptibility of normal human b lymphocyte populations. | human blood and tonsil b lymphocytes were fractionated on density gradients and tested for virus binding and penetration into the cells. epstein-barr virus (ebv) transformation was detected by immunofluorescence staining for ebv-determined nuclear antigen (ebna). ebv bound to and penetrated all b cell populations, but only the high density populations were transformed. activated b lymphocytes were found in the low density fractions and these cells were resistant to ebv infection. infected and no ... | 1984 | 6319530 |
| papular acrodermatitis with epstein-barr virus infection. | a case of papular acrodermatitis (pac) associated with epstein-barr virus infection occurred in a 9-month-old child. current knowledge of the possible role of various viral antigens in pac is discussed. | 1984 | 6317266 |
| a familial syndrome of susceptibility to chronic active epstein-barr virus infection. | in two members of a family (daughter and father) active epstein-barr virus (ebv) infections persisted over periods of 4 and 3 years respectively (possibly 10 years in the father). both had persistent splenomegaly and occasional bouts of unexplained fever but lived otherwise normal lives. the other members of the family (mother and son) were healthy. the titres of antibody to the ebv viral capsid antigen (vca) and early antigen (ea) were extremely high in the daughter's blood, whereas the titres ... | 1984 | 6318944 |
| titers of epstein-barr virus-related antibodies in nasopharyngeal carcinoma in japan. | it is thought that nonkeratinizing or undifferentiated squamous cell carcinoma in the nasopharynx (npc) is intimately correlated with epstein-barr virus (ebv). twenty-one patients with npc were followed in kyoto university hospital and 4 in osaka red cross hospital during the past 2 years from 1980 to 1981. these patients were classified histopathologically according to the who classification in 1978 and staged with the tnm classification in union internationale contre le cancer (uicc) in 1978. ... | 1984 | 6318948 |
| chromosomes and causation of human cancer and leukemia. lii. chromosome findings in treated patients with b-cell chronic lymphocytic leukemia. | the chromosomes of stimulated lymphocytes in 40 treated cases of b-cell chronic lymphocytic leukemia (b-cll) were examined. the polyclonal b-cell activators (pba) used were: pokeweed mitogen (pwm), epstein-barr virus (ebv), and lipopolysaccharide w from escherichia coli 055:b5 (lps). thirty-three (83%) of the 40 cases contained an adequate number of metaphases that were suitable for banding. of 15 cases with abnormal clones, 7 cases had trisomy #12. occasionally, trisomy #1, 6q-, i(7q), 14q+, tr ... | 1984 | 6318964 |
| immunological characterization of hodgkin's and non-hodgkin's lymphoma patients with high antibody titers against epstein-barr virus-associated antigens. | we have studied nine hodgkin's lymphoma (hd) and ten non-hodgkin's lymphoma (nhl) patients with extraordinarily high anti-viral capsid antigen (vca) titers (greater than 5120). controls were 13 hd and 23 nhl patients with anti-vca titers between 40 and 2560. high anti-vca titers were present in nhl patients at the time of diagnosis or within 16 months, whereas the rise of anti-vca titers in hd patients appeared to be a late event during the clinical course of the disease (mean time from diagnosi ... | 1984 | 6318984 |
| effect of indomethacin on epstein-barr virus early antigen induction. | the lymphoid cell line, raji, was derived from a burkitt's lymphoma and is readily inducible for epstein-barr virus (ebv) early antigen synthesis by the tumor promoter 12-o-tetradecanoylphorbol-13-acetate. treatment of raji and other ebv genome-positive cells with indomethacin caused a marked inhibition of early antigen induction by 12-o-tetradecanoylphorbol-13-acetate and other chemical inducers. however, this effect did not appear to be due to inhibition of prostaglandin synthesis since the co ... | 1984 | 6318991 |
| egna-specific lif production of human lymphocyte subsets. | using the indirect leukocyte migration inhibition technique t cells have been identified as being responsible for epstein-barr virus nuclear antigen-induced specific leukocyte migration inhibitory factor production. the response was dependent on the presence of macrophages or their product, t-lymphocyte activating factor. | 1984 | 6319032 |
| humoral immune response in epstein-barr virus infections. ii. igg subclass distribution in african patients with burkitt's lymphoma and nasopharyngeal carcinoma. | native burkitt's lymphoma (bl) and nasopharyngeal carcinoma (npc) patients from kenya were examined with regard to the serum concentrations and distribution of the four igg subclasses, total igg, and antibody activities to epstein-barr (eb) virus associated antigens. the results were compared with corresponding data of an african control group. as revealed by indirect immunofluorescence techniques, the patients displayed a pattern of igg and iga antibodies to eb virus associated antigens which i ... | 1984 | 6319056 |
| epstein-barr virus induced pneumococcal antibody production in man. a comparison of different lymphoid organs. | b cell enriched lymphocytes from different lymphoid organs were compared regarding their ability to produce pneumococcal polysaccharide (pps) antibodies in vitro after transformation with epstein-barr virus. tonsillar, adenoidal and peripheral blood lymphocytes (pbl) were studied. fifty thousand b enriched lymphocytes from each organ sample were cultured in each of 88 wells of a microplate. after 30 days the supernatant of each culture was assayed for pneumococcal antibody activity and total igg ... | 1984 | 6319057 |
| in vitro stimulated lymphocytes as a source of human hybridomas. | stimulation of peripheral blood lymphocyte (pbl) cultures from tetanus toxoid (tt)-immunized donor with epstein-barr virus (ebv) yielded cells with much higher frequencies of hybrid formation (36 x 10(-7) compared to unstimulated pbl or cells cultured with pokeweed mitogen or tt antigen. the proportion of hybridomas (approximately 1%) producing anti-tt antibody was similar in ebv- and tt-stimulated cultures. a marked increase in immunoglobulin secretion was observed after hybridization and prese ... | 1984 | 6319153 |
| primary structural sequence polymorphism in the human class ii mhc antigen 33.1. | monoclonal antibody 33.1 defines a non-dr, class ii, human major histocompatibility complex antigen, 33.1, which appears to be distinct from other class ii antigens in its cellular distribution and primary structure. to characterize the structure more fully and to determine the degree of polymorphism within 33.1, a comparative n-terminal sequence study has been undertaken using a series of ten b lymphoblastoid cell lines with different dr and mb types. the results confirm that both the alpha and ... | 1984 | 6319275 |
| cross-linking of cell surface immunoglobulins induces epstein-barr virus in burkitt lymphoma lines. | anti-human immunoglobulin (ig) antibodies with single-chain specificities induced epstein-barr virus (ebv) in various burkitt lymphoma lines when the corresponding ig chain was expressed on the cell surface. the f(ab')2 fragments of igg antibody were as potent as intact ig, while the fab and fc fragments gave no induction, indicating that cross-linking of surface ig was required for the induction. simultaneously with ebv induction, anti-ig inhibited the uptake of 3h-thymidine. this inhibition wa ... | 1984 | 6319296 |
| hla-dr-antigen-associated restriction of ebv-specific cytotoxic t-cell colonies. | ebv-specific cytotoxic t cells can be generated in vitro in a secondary response. several previous studies with bulk cultures provided evidence that cytotoxicity was restricted by hla-a,b-related antigens. in the present family study, the ebv-specific cytotoxic t-cell response of a normal ebv-seropositive donor was analysed in detail by t-cell colony formation. peripheral blood mononuclear cells were stimulated by the gamma-irradiated autologous lymphoblastoid cell line (lcl) and 3 days later se ... | 1984 | 6319303 |
| availability of mo and htlv-ii. | 1984 | 6322000 | |
| burkitt's lymphoma in cuba. i. clinical and morphological features and ebv association. | the first 7 cases of histologically and cytochemically confirmed burkitt's lymphoma have recently been studied in cuba. some of them presented with facial bone involvement and high anti-epstein-barr virus (ebv) antibody titers characteristic of endemic african cases. others showed features of nonendemic cases. the distribution of patients in these two groups may be in relation to the behavior of the healthy cuban population with respect to the ebv virus. | 1984 | 6322085 |
| kawasaki disease and an unusual primary infection with epstein-barr virus. | 1984 | 6322099 | |
| fatal epstein-barr virus disease in a laotian refugee. | 1984 | 6322142 | |
| two deletions in the epstein-barr virus genome of the burkitt lymphoma nonproducer line raji. | the epstein-barr virus genome carried in the burkitt lymphoma nonproducer cell line raji was characterized by partial denaturation mapping and by hybridization of cloned viral fragments to filters containing separated raji dna fragments. partial denaturation mapping revealed that the ebv dna population of raji cells is homogeneous and that two deletions are observed in distant parts of the genome compared to linear dna isolated from virus particles of different strains. these deletions were char ... | 1984 | 6322426 |
| infection of mouse lymphocytes by epstein-barr virus. ii. stimulation of cellular dna synthesis by ebv in the absence of ebna induction and cell transformation. | epstein-barr virus (ebv) of the transforming and nontransforming strains induced transient stimulation of cellular dna synthesis during lytic infection of normal mouse lymphocytes. in contrast to human b lymphocytes, the action of both ebv strains in mouse cells was additive. the nontransforming p3hr-1 virus had no cytotoxic effect on mouse lymphocytes. the ebv-infected, stimulated mouse lymphocytes did not express eb virus-determined nuclear antigen and do not grow into immortalized cell lines. ... | 1984 | 6322431 |
| the association of extrahepatic bile duct atresia and neonatal epstein-barr virus infection. | an infant with uncorrectable extrahepatic bile duct atresia was found to have evidence of epstein-barr virus infection during the neonatal period. it is probable that the infection was acquired in utero. in view of the association of hepatitis with the epstein-barr virus in later life, it is possible that this infection was responsible for the development of bile duct obstruction. | 1984 | 6322509 |
| failure to acquire epstein-barr virus infection after intimate exposure to the virus. | in 1981, 54 epstein-barr virus-seronegative pregnant women in the sacramento-davis area of california were observed prospectively for an average duration of 12.1 months for epstein-barr virus-seroconversion. only one seroconverted. two of the 54 women remained negative for epstein-barr virus antibody 4.5-5.0 months after their husbands were proved to be epstein-barr virus excreters. short of experimental pharyngeal introduction of the virus into human volunteers, these data provide the most dire ... | 1984 | 6322579 |
| epstein-barr virus-associated myelopathy in an adult. | a 55-year-old man had a myelopathic syndrome and serologic evidence of epstein-barr virus infection. the patient's age and the neurologic and serologic findings suggested reactivation of a latent virus with direct invasion of the cns. epstein-barr virus may exist in a latent state within the nervous system, and should be considered in myelopathic syndromes, even in older patients. | 1984 | 6322729 |
| epstein--barr virus nuclear antigen in a non-hodgkin's lymphoma. | this paper describes the course of a patient with treated non-hodgkin's lymphoma, which originally presented as a large nasopharyngeal mass. the tumor, though irradiated, recurred on two separate occasions in the right and left inguinal regions. at the time of tumor recurrence, markers were present which have been associated with past or recurrent epstein-barr virus (ebv) infection, namely, antibodies to the ebv viral capsid antigen (vca) and the epstein-barr nuclear antigen (ebna). ebna was det ... | 1984 | 6322937 |
| heat durability of epstein-barr virus-activating substances of plant origin: 12-o-tetradecanoylphorbol-13-acetate, 12-o-hexadecanoyl-16-hydrophorbol-13-acetate, croton oil, tung oil and croton megalocarpus extract. | 12-o-tetradecanoyl-phorbol-13-acetate (tpa), 12-o-hexadecanoyl-16-hydrophorbol-13-acetate (hhpa), croton oil, tung oil and croton megalocarpus ether extract, all of which are known to possess epstein-barr virus-activating potency, retained their capacity to induce epstein-barr virus early antigen complex in the viral genome-carrying human lymphoblastoid cells (raji) even after heat treatment at 120 degrees c for 2 h or at 100 degrees c for 12 h. such unusual heat resistance of the agents tested ... | 1984 | 6322973 |
| the challenge of herpesviruses. | 1984 | 6322975 | |
| antagonistic action of retinoic acid against teleocidin and 12-o-tetradecanoyl-phorbol-13-acetate on activation of epstein-barr virus genomes. | teleocidin, a new potent tumor promoter, produces biological effects similar to 12-o-tetradecanoyl-phorbol-13-acetate (tpa) on human lymphoblastoid cell lines. a wide range of concentrations (4-16 ng/ml) of teleocidin were optimal for induction of replication of latent epstein-barr virus (ebv) genomes in p3hr-1 cells; cell growth was significantly inhibited in this dose range. in contrast, treatment of raji cells with an identical dose range of teleocidin did not induce replication of latent ebv ... | 1984 | 6323048 |
| chromosomal localisation of the human homologues to the oncogenes erba and b. | avian erythroblastosis virus (aev) induces acute erythroleukemia and sarcomas in vivo and it transforms erythroblasts and fibroblasts in vitro. the virus has two host cell-derived genes, v-erba and v-erbb. the latter encodes the oncogenic capacity of the virus, whereas v-erba enhances the erythroblast transforming effects of v-erbb while being unable to induce neoplasms independently. recently, human cellular homologues of these viral erb genes have been isolated. the chromosomal locations of tw ... | 1984 | 6323162 |
| searching for the cause of the acquired immune deficiency syndrome. | an outbreak of unexplained immune deficiency associated with opportunistic infection and kaposi's sarcoma is occurring in the usa and other parts of the world. affected individuals with what had come to be known as the acquired immune deficiency syndrome (aids) have a high mortality. epidemiological features suggest the presence of a transmissable agent, but no responsible agent has yet been identified. homosexual and bisexual men make up 75% of these affected individuals. cytomegalovirus, epste ... | 1984 | 6323176 |
| induction of epstein-barr virus antigens by tumor promoters for epidermal and nonepidermal tissues. | fifteen established tumor promoters belonging to different chemical groups were tested for their ability to induce epstein-barr virus (ebv)-specific early antigens (ea) in ebv-genome-positive nonproducer raji cells. saccharin (a promoter in urinary bladder carcinogenesis), ddt (a promoter in liver carcinogenesis), anthralin and iodoacetic acid (promoters in skin carcinogenesis) gave a significant induction with a maximum of induced cells of 20% (8 mg/ml), 0.8% (20 micrograms/ml), 0.8% (100 ng/ml ... | 1984 | 6323326 |
| epstein-barr virus binding to virus-carrying cell lines is enhanced in the presence of c3 and c3d. | the relationship between the receptors for the epstein-barr virus (ebv) and the c3d fragment of complement was investigated at the molecular level. in the presence of cell-bound c3, virus binding was enhanced in ebv genome-carrying lines. an identical effect could be elicited by c3d at one-quarter the weight amount; c3b and methylamine-treated c3 had no effect on virus binding. the minimum concentration of c3 which produced significant enhancement was 25 micrograms/ml. virus binding increases we ... | 1984 | 6321638 |
| epstein-barr virus with heterogeneous dna disrupts latency. | by cloning the hr-1 burkitt lymphoma line, we previously uncovered two distinct biological variants of nontransforming epstein-barr virus (ebv). the most commonly cloned variant has a low rate of spontaneous viral synthesis and is unable to induce early antigen in raji cells (eai-). a rare variant spontaneously releases virus which is capable of inducing early antigen in raji cells (eai+). since eai- virus lacks heterogeneous dna (het-) and eai+ virus contains heterogeneous dna (het+), we sugges ... | 1984 | 6321789 |
| detection of circular and linear herpesvirus dna molecules in mammalian cells by gel electrophoresis. | a simple gel technique is described for the detection of large, covalently closed, circular dna molecules in eucaryotic cells. the procedure is based on the electrophoretic technique of eckhardt (t. eckhardt, plasmid 1:584-588, 1978) for detecting bacterial plasmids and has been modified for the detection of circular and linear extrachromosomal herpesvirus genomes in mammalian cells. gentle lysis of suspended cells in the well of an agarose gel followed by high-voltage electrophoresis allows sep ... | 1984 | 6321792 |
| prolonged inhibitory effect of 9-(1,3-dihydroxy-2-propoxymethyl)guanine against replication of epstein-barr virus. | the effects of 9-(1,3-dihydroxy-2-propoxymethyl)guanine (dhpg), a new antiviral drug, and acyclovir (acv) [9-(2-hydroxyethoxymethyl)guanine] on the replication of epstein-barr virus (ebv) were compared. both drugs inhibited ebv dna replication in p3hr-1 cells and superinfected raji cells, but neither inhibited replication of the plasmid form of the ebv genome in latently infected raji cells. however, dhpg had a more prolonged inhibitory effect than acv. although the effect of the drugs is prompt ... | 1984 | 6321799 |
| the relation of cytomegalovirus and epstein-barr virus antibodies to t-cell subsets in homosexually active men. | eighty-nine homosexual men participated in a study of sexual practices, t-cell subsets, and antibody titers to cytomegalovirus (cmv) and epstein-barr virus (ebv). the prevalence of antibody to cmv was 96% and to ebv, 94%. titers to cmv were higher in those practicing receptive anal intercourse. the cmv antibody titers were positively correlated with the t-helper/suppressor ratio, number of partners and venereal disease episodes, number of t-suppressor cells, and ebv titers. twelve percent of the ... | 1984 | 6321811 |
| characterization of epstein-barr virus-related thymidine kinase induced in nonproducer cells by superinfection or chemical treatment. | chemical treatment with a combination of 12-o-tetradecanoylphorbol-13-acetate and n-butyrate or superinfection with p3hr-1 virus effectively induced a novel thymidine kinase (tk) in raji and nc37 tk-negative mutant cells. sera from patients with nasopharyngeal carcinoma containing a high antibody titer against epstein-barr virus early antigen could inactivate this induced enzyme while sera from normal donors, even those containing a high titer of antibodies to virus capsid antigen, had no effect ... | 1984 | 6321393 |
| lymphocyte ecto-5'-nucleotidase activity in infancy: increasing activity in peripheral blood b cells precedes their ability to synthesize igg in vitro. | ecto-5'-nucleotidase activity was measured in peripheral blood lymphocytes isolated from serial specimens from nine healthy full-term infants and two premature infants at 0, 2, 4, and 6 mo of age. the postnatal nadir in activity was 7.1 +/- 2.0 nmol/hr/10(6) cells, which is the same as the activity in cord blood lymphocytes (7.0 +/- 2 nmol/hr/10(6) cells). the activity rose twofold to 13.2 +/- 3.8 nmol/hr/10(6) cells at 6 mo of age (p less than 0.001, paired t-test), which is similar to the acti ... | 1984 | 6321590 |
| a sensitive enzyme-linked immunosorbent assay (elisa) against the major ebv-associated antigens. i. correlation between elisa and immunofluorescence titers using purified antigens. | an enzyme-linked immunosorbent assay (elisa) was developed for the major epstein-barr virus (ebv) associated antigens. the elisa assay was based on purified protein components of virus-capsid antigen (vca), early antigen (ea), membrane antigen (ma) and the nuclear antigen (ebna). these antigens, except ebna, were purified on immunoaffinity columns containing monoclonal antibodies, while ebna was purified by biochemical methods. screening of 69 human sera including 26 sera from individuals with i ... | 1984 | 6321599 |
| a flow cytometric technique for quantitation of b-cell activation. | a flow cytometric method for enumeration of intracytoplasmic antibody-containing cells was developed. flow cytometry was found to yield results comparable to traditional fluorescence microscopy in cells stimulated to produce intracytoplasmic antibody by either pokeweed mitogen or epstein-barr virus. this technique offers several advantages over traditional fluorescence microscopy, including objective measurement of fluorescence intensity and rapid analysis of large numbers of cells. | 1984 | 6321600 |
| epstein-barr serology in immunodeficiencies: an attempt to correlate with immune abnormalities in wiskott-aldrich and chediak-higashi syndromes and ataxia telangiectasia. | epstein-barr (eb) virus serology was correlated with the results of immunological investigations of three inherited immunodeficiency diseases, in an attempt to understand the immune mechanisms controlling eb virus infection. in nine patients with wiskott-aldrich syndrome (was), the constant lack of anti-eb virus associated nuclear antigen (ebna) was accompanied by a consistent impairment of allogeneic cytotoxicity. we confirmed a frequent absence of anti-ebna antibody in ataxia telangiectasia (a ... | 1984 | 6321070 |
| virus-associated hemophagocytic syndrome due to epstein-barr virus. | the clinical and autopsy findings in a case of virus-associated hemophagocytic syndrome in a previously healthy 17-year-old girl are presented. serum titers to epstein-barr viral antigens were unusually elevated. in addition to widespread proliferation of hemophagocytic histiocytes and lymphoid depletion, there were areas of non-suppurative necrosis in lymph nodes and spleen, which have not been previously reported. | 1984 | 6321323 |
| prevention of epstein-barr virus-induced b-cell outgrowth by interferon alpha. | an in vitro system for determining the efficacy of interferon alpha (ifn-alpha) in preventing b-cell outgrowth due to epstein-barr virus (ebv) was developed. unfractionated cord blood mononuclear cells, t-cell-depleted cord blood mononuclear cells, or adult t-cell-depleted mononuclear cells were exposed to ifn-alpha for 18 to 20 h followed by incubation with the b95-8 strain of ebv for 2 h. b-cell outgrowth was monitored by microscopic examination, [3h]thymidine incorporation, and epstein-barr n ... | 1984 | 6321354 |
| studies on chemical carcinogen enhancement of epstein-barr virus induced transformation of human neonatal and adult peripheral blood lymphocytes. | the effects of a wide range of selected chemical carcinogens on the frequency of epstein-barr virus (ebv)-induced transformation have been investigated. the carcinogens tested included direct-acting chemicals and chemicals requiring either activation via reactions with nucleophiles, or cell-mediated enzyme activation. treatment with some but not all chemicals suspected of being carcinogens resulted in enhanced ebv-induced transformation of neonatal or adult peripheral blood lymphocytes (pbls). t ... | 1984 | 6321362 |
| in vitro production of monoclonal human rheumatoid factors. | lymphocytes from rheumatoid arthritis patients have been fused with drug-marked human myeloma cell lines and hybrids selected which secrete rheumatoid factors. limiting dilution and soft agarose plates have been used to clone these hybrids, but so far stable clones have not been obtained. epstein-barr virus has been used to transform lymphocytes, and clones which secrete rheumatoid factors have been obtained. | 1984 | 6320710 |
| effect of sera from patients with hodgkin's disease on normal donor lymphocytes containing parallel tubular structures. | normal donor peripheral blood mononuclear cells incubated for 24 h with sera from patients with hodgkin's disease were investigated by electron microscopy for the presence of parallel tubular structures (pts) and/or amorphous electrondense granules (large granular lymphocytes = lgl). in comparison with normal human serum, 14 out of 29 sera of the patients induced a marked increase in the percentage of lgl. from a limited number of experiments it was likely that this increase is paralleled by an ... | 1984 | 6320933 |
| immunologic studies of peripheral blood in a child with hypogammaglobulinemia. suggested mechanism for the development of malignant b-cell lymphoma. | a case of a 7-year-old boy with common variable hypogammaglobulinemia who developed b-cell-type non-hodgkin's lymphoma is reported. immunologic studies of his peripheral blood before the development of lymphoma revealed: (1) although peripheral t-cell and b-cell counts were normal, serum igg and iga levels were remarkably reduced; (2) dna synthesis in response to phytohemagglutinin-p (pha), concanavalin a (con a), and pokeweed mitogen (pwm) stimulation were decreased; (3) dna synthesis in respon ... | 1984 | 6321007 |
| in vitro infection of human b lymphocytes with adult t-cell leukemia virus. | experimental transmission of adult t-cell leukemia (atl) virus (atlv) into human b lymphocytes was attempted. cocultivation of b-cell rich fraction of peripheral blood from a healthy adult with x-ray irradiated atlv producer mt-2 cells resulted in the establishment of oka(b) cell line co-infected with both epstein-barr virus (ebv) and atlv. oka(b) cells and its subclones contained: (1) b cell markers exclusively; (2) both ebv-specific antigen, ebna and atlv-specific antigen, atla detected by imm ... | 1984 | 6321019 |
| detection of s-100 labelled cells in nasopharyngeal carcinoma. | s-100 antigen containing cells with dendritic features, recognisable by morphological and immunohistochemical criteria as belonging to the langerhans' or interdigitating reticulum cell type, have been found in undifferentiated nasopharyngeal carcinoma. the presence of these cells, which have a special function of antigen presentation in immune responses, may be involved in a possible modulation of nasopharyngeal carcinoma associated epstein-barr virus infection and host-tumour interactions. | 1984 | 6389603 |
| production of monoclonal antibody to a late intracellular epstein-barr virus-induced antigen. | a monoclonal antibody designated l2 was produced against a late intracellular protein induced by epstein-barr virus (ebv). this protein was expressed in cells producing virus but not in ebv genome-positive nonproducer cell lines, ebv genome-negative cell lines, or producer cultures cultivated in the presence of phosphonoacetic acid as determined by immunofluorescence. in addition, the antibody did not react with the membranes of infected cells indicating that it was not directed against an ebv-i ... | 1984 | 6324457 |
| the five human herpesviruses: infection, prevention, and treatment. | 1984 | 6324554 | |
| two distinct antigen systems in human b lymphocytes: identification of cell surface and intracellular antigens using monoclonal antibodies. | two distinct antigen systems (l26 and l27) specifically expressed in human b lymphocytes were identified using tb2-2b3 (2b3) and t3-5b3 (5b3) monoclonal antibodies, respectively. whereas l26 antigen defined by 2b3 were rarely expressed on the surface of b cells but abundant in the cytoplasm, 127 antigens detected by 5b3 was clearly expressed on the cell surface. these two antigens appeared to be restricted in their expression to b cells, as they were found in most b cells but not other cell type ... | 1984 | 6332692 |
| 2',5'-oligoadenylate trimer core and the cordycepin analog augment the tumoricidal activity of human natural killer cells. | interferon (ifn) augments the lytic activity of natural killer (nk) cells, inhibits the transformation of human peripheral blood lymphocytes (pbl) by epstein barr virus (ebv), and induces a 2',5'-oligoadenylate (2',5'-an) synthetase. exogenous 2',5'-an by itself can inhibit the transformation of human pbl by ebv. the present studies report that 2',5'-an and its cordycepin analog also augmented the tumoricidal activity of human nk cells. incubation of nylon wool-passed pbl for 1 to 2 hr with the ... | 1984 | 6332860 |
| release of platelet-activating factor (paf)-like material from human lymphoid cell lines. | the release of platelet-activating factor (paf)-like material was evaluated from eight b and t human leukemic cell lines and from three lymphoblastoid cell lines obtained after epstein-barr virus (ebv) transformation. all cell lines were stimulated with ionophore a23187, phytohemagglutinin (pha), or both either in the presence of or without acetyl coenzyme a (accoa). after appropriate stimuli, all cell lines tested released paf-like material, irrespective of the surface phenotype and of the leve ... | 1984 | 6333353 |
| tyrosine kinase activity of the insulin receptor of patients with type a extreme insulin resistance: studies with circulating mononuclear cells and cultured lymphocytes. | the syndrome of type a insulin resistance in nonobese women is characterized by hyperinsulinemia, resistance to exogenous insulin, acanthosis nigricans, polycystic ovaries, and masculinization. insulin binding to intact circulating monocytes and cultured epstein-barr virus-transformed b-lymphocytes derived from these patients is decreased in some patients but normal in others. insulin receptors consist of two subunits; the alpha-subunit contains the insulin-binding site, and the beta-subunit pos ... | 1984 | 6333431 |
| a sensitive assay for detecting hypersensitivity to ionizing radiation in lymphoblastoid lines from patients with duchenne muscular dystrophy and primary neuronal degenerations. | hypersensitivity to the lethal effects of dna-damaging agents is usually demonstrated using the classical colony-forming ability assay with cultured fibroblast lines. based on the ability of viable cells in lymphoblastoid lines (epstein-barr virus-transformed b lymphocytes) to exclude the vital dye trypan blue, we have developed a more rapid survival assay which has been useful in detecting hypersensitivity to ionizing radiation in certain diseases characterized by primary degeneration of excita ... | 1984 | 6333487 |
| a 14;18 and an 8;14 chromosome translocation in a cell line derived from an acute b-cell leukemia. | we have established a cell line, which we named 380, from a young male with acute lymphoblastic leukemia (fab type l2). karyologic analysis of this cell line indicates that it carries an 8;14 and a 14;18 chromosome translocation, which are characteristic of burkitt lymphoma and of follicular lymphoma, respectively. this cell line is epstein-barr virus antigen-negative, reacts with monoclonal antibodies specific for b cells, and contains rearranged immunoglobulin heavy and light chain genes, but ... | 1984 | 6334305 |
| establishment of a new peroxidase-positive human myeloid cell line, pl-21. | a myeloid cell line, designated pl-21, was established from the peripheral blood of a patient with acute promyelocytic leukemia. the pl-21 cell line grew in single-cell suspension, with a doubling time of 48-64 hr, and consisted of promyelocytes with fine immature nuclei and prominent azurophilic granules in the cytoplasm. pl-21 cells were positive for peroxidase, naphthol as-d chloroacetate esterase, and sudan black b staining. under the usual culture conditions, a small proportion of these cel ... | 1984 | 6362749 |
| reduced levels of fibronectin in ataxia-telangiectasia lymphoblastoid cells. | in this study epstein-barr virus-transformed lymphoblastoid cells were shown to contain fibronectin. a marked difference in the number of fibronectin-positive cells was observed in control and ataxia-telangiectasia lymphoblastoid cells. three control cell lines varied between 60% and 80% in the number of cells containing fibronectin. only a small percentage of ataxia-telangiectasia cells (1-5%) had fibronectin present. control and ataxia-telangiectasia fibroblasts had similar amounts and distrib ... | 1984 | 6365801 |
| incidence of beta hemolytic streptococcal pharyngitis in adolescent with infectious mononucleosis. | reports on the incidence of beta-hemolytic group a streptococci (bhgas) in the pharynx of patients with infectious mononucleosis (im) have varied from 3% to 33%. to ascertain the rate of infection, we prospectively performed serial throat cultures and determined anti-streptococcal antibody titers on 45 students with confirmed im by epstein-barr virus-specific serology. one hundred healthy control students had throat cultures for comparison. the rate of recovery of bhgas was similar in patients w ... | 1984 | 6368507 |
| detection of iga antibodies to epstein-barr virus-associated antigens by elisa. | the detection of iga antibodies to the epstein-barr virus (ebv)-associated viral capsid antigen (vca) and early antigens (ea) is of diagnostic and prognostic importance for patients with nasopharyngeal carcinoma (npc). an elisa for the determination of serum igg antibodies to these antigens has been developed which uses the double antibody method. 136 sera obtained from healthy donors and patients with non-ebv related tumors and lymphomas were tested by elisa; only 3 sera, from patients with chr ... | 1984 | 6368695 |
| is cancer communicable? | recent developments in cancer epidemiology have led to the possibility of an exceedingly complex communicable factor(s) in cancer etiology. the transmission of such an agent(s) may require a susceptible genotype and/or other promotional events. likely candidates which support this supposition include: epstein-barr virus (nasopharyngeal carcinoma, burkitt's lymphoma, salivary gland tumor among eskimos, x-linked lymphoproliferative syndrome of purtilo); human t-cell leukemia virus (adult t-cell le ... | 1984 | 6379396 |
| analysis of insulin receptors on human lymphoblastoid cell lines by flow cytometry. | antibodies to the insulin receptor have provided important experimental probes of receptor structure and function. in the present study, we have characterized the insulin receptor on human lymphoblastoid cell lines using polyclonal and monoclonal anti-receptor antibodies and fluorescence flow cytometry. the cell lines were derived by epstein-barr virus transformation of peripheral mononuclear leucocytes from normal subjects or patients with disorders that affect the insulin receptor. fluorescenc ... | 1984 | 6383914 |
| induction of cellular dna polymerases in a burkitt lymphoma-derived cell line by treatment with 5-iododeoxyuridine. | cellular dna polymerases of a burkitt lymphoma-derived cell line (p3hr-1) were found to be greatly induced by treatment of the cells with 5-iododeoxyuridine (iudr) at a concentration which induces epstein-barr virus (ebv) early antigen (ea) expression. the activities of all the dna polymerases alpha, beta and gamma in p3hr-1 cells increased 7-9 fold by exposure of the cells to iudr (25 micrograms/ml) for 3 days, while the ebv-coded dna polymerase activity in the cell remained undetectable under ... | 1984 | 6385973 |
| secondary malignancies after marrow transplantation. | secondary malignancies after marrow transplantation have been observed in 20 patients: 19 patients underwent marrow transplantation for the treatment of a hemopoietic malignancy and one for aplastic anemia. all but three were given total body irradiation at doses of 8.0-15.75 gy as part of the conditioning regimen. secondary malignancies were composed of three groups: (a) six patients had recurrence of leukemia (three acute lymphoblastic, two acute myeloblastic, and one chronic myelocytic) in ce ... | 1984 | 6386505 |
| cellular transforming genes and oncogenesis. | 1984 | 6331501 | |
| characterization of a novel htlv-infected cell line. | a man from chile developed an aggressive mature t cell leukemia associated with marked eosinophilia. the neoplastic lymphocytes were of t helper surface phenotype, and they expressed the p24 and p19 antigens of human t cell leukemia virus (htlv). a cell line (me) was established from the patient's peripheral blood cells that was initially composed of eosinophils and t and b lymphocytes. the b lymphocytes of the cell line are polyclonal and contain epstein-barr virus (ebv) dna. many of the t lymp ... | 1984 | 6331549 |
| epstein-barr (eb) virus infection in homosexual men in london. | twenty five homosexual men from london, 14 of whom had persistent lymphadenopathy and 11 of whom did not, were tested for immunity to epstein-barr (eb) virus. all yielded positive results to serological tests for the viral capsid antibody, and 11 had antibodies to the early antigen. thirteen out of 17 were excreting virus into the saliva; culture of peripheral blood mononuclear cells from two of these patients showed no detectable regression induced by t cells that was specific to eb virus. no d ... | 1984 | 6331569 |
| epstein-barr virus activation in raji cells with ether extracts of soil from different areas in china. | epstein-barr virus (ebv) inducers were found in soil samples collected from the ground under tung oil trees and other plant species of euphorbiaceae family growing in southern provinces of china where incidence of an ebv-associated malignancy, nasopharyngeal carcinoma (npc) is prevalent. in such npc high risk areas, the positive rate of ebv inducers in soil samples was up to 59.5%. since many tung oil trees are planted along the roads and rivers of the high risk area, the possible significance o ... | 1984 | 6331635 |
| epstein-barr virus-activating principle in human semen. | during the screening of natural and physiological products for their epstein-barr virus (ebv)-activating potency, we found that a considerable number of human semen specimens obtained from infertility clinics (30 cases) possess a marked capacity to induce ebv early antigen (ea) in non-producer raji cell system when assayed in combination with n-butyrate. the ebv ea-inducing activity of the semen samples was comparable to that of the most efficient ebv ea inducers, the plant diterpene esters such ... | 1984 | 6331637 |
| activation of human t lymphocytes ii. involvement of the t3 antigen in polyclonal t cell activation by mitogenic lectins and oxidation. | the effect of monoclonal antibodies against the t cell surface antigens t3 and t4 on accessory cell-dependent mitogen-induced proliferation of human antigen-specific t4+ t lymphocyte clones and purified peripheral blood t cells was studied. to avoid the fc receptor-dependent mitogenic effects of the okt3 antibodies, monocytes were replaced by epstein-barr virus-transformed b lymphoblastoid cells as accessory cells. monoclonal antibody okt3 but not okt4 inhibited the response of both t lymphocyte ... | 1984 | 6332027 |
| [humoral response to epstein-barr virus of patients with nasopharyngeal cancer and its use in the differential diagnosis of the disease]. | 1984 | 6331022 | |
| epstein-barr virus-induced lymphoma in a cardiac transplant recipient. | a monoclonal diffuse histiocytic lymphoma developed during the course of a serologically documented primary epstein-barr virus infection in a 22-year-old cardiac transplant recipient taking cyclosporine and prednisone. throat washings revealed the virus at tumor presentation, and the tumor was shown to contain epstein-barr nuclear antigen-positive cells and the viral genome. prolonged inversion of the t cell helper/suppressor ratio was demonstrated. a brief course of acyclovir appeared to halt v ... | 1984 | 6331161 |
| delayed onset of infectious mononucleosis associated with acquired agammaglobulinemia and red cell aplasia. | in 1974, an 11-year-old white boy with the x-linked lymphoproliferative syndrome developed hyper-igm after becoming infected with epstein-barr virus. however, he failed to develop normal immune responses against the virus. in december 1981, when red cell aplasia occurred, he was given packed erythrocytes and gammaglobulin. nine weeks later, acute infectious mononucleosis developed. concurrently, his t4/t8 helper/suppressor ratio decreased from 2.7 to 0.2, and igm antibodies to epstein-barr virus ... | 1984 | 6331239 |
| ophthalmoplegic polyneuropathy: another case in association with epstein-barr virus. | 1984 | 6331278 | |
| epstein-barr virus replication in oropharyngeal epithelial cells. | despite the well-established tropism of the epstein-barr virus (ebv) for human b lymphocytes, the cell type within the oropharynx capable of allowing ebv replication has never been conclusively identified. using in situ cytohybridization, we demonstrated ebv dna in oropharyngeal epithelial cells from 10 of 12 patients with infectious mononucleosis. in duplicates of specimens found to contain cell-associated ebv dna, we detected ebv rna in two of four samples, using a biotin-labeled ebv dna probe ... | 1984 | 6323983 |
| epstein-barr virus--immortalization and replication. | 1984 | 6323984 | |
| production of human monoclonal igg antibodies against rhesus (d) antigen. | an epstein-barr virus (ebv)-transformed human b-cell line ( lb4r ) producing anti-rhesus [rho(d) antigen] antibody was fused with a non-immunoglobulin-producing mouse-human heteromyeloma ( shm - d33 ) and selected in hypoxanthine/aminopterin/thymidine medium containing 0.5 microm ouabain. surviving hybrids found to secrete specific anti-rho(d) antibody were cloned by limiting dilution. two clones (d4-b2 and e10-c1) producing high levels (12 and 20 micrograms/ml per 10(6) cells per 24 hr, respect ... | 1984 | 6427767 |
| immunological alterations in haemophiliacs treated with lyophilized factor viii cryoprecipitate from volunteer donors. | we studied immune function in belgian haemophiliacs treated with factor viii from volunteer donors. no patient had clinical evidence of immune deficiency. we found a decrease in t-helper cells (p less than 0.0005), in the ratio of t-helper over t-cytotoxic/suppressor cells (1.72 +/- 0.47 versus 2.24 +/- 0.82 in controls, p less than 0.005) and in lymphocyte responsiveness to mitogens (p less than 0.05). these findings could not be linked to the amount of f viii received over the last year, the t ... | 1984 | 6429883 |
| electron microscopic evidence of non-a, non-b hepatitis markers and virus-like particles in immunocompromised humans. | characteristic pathological alterations of the liver in chimpanzees inoculated with non-a, non-b hepatitis sera have been described, but no corresponding findings have been reported in humans. electron microscopic studies of the liver biopsy specimens of two homosexual patients with acquired immune deficiency syndrome, one without hepatitis (patient 1) and one with chronic active hepatitis in remission (patient 2), revealed the cytoplasmic tubular structures which are characteristic of chimpanze ... | 1984 | 6430774 |
| establishment of human monoclonal anti-dna antibody producing cell lines. | we developed a useful method for the establishment of stable cell lines producing human monoclonal anti-dna antibody by in vitro epstein-barr virus infection. the practical limitation for the cloning was overcome by 2 procedures. one was a microculture system using a small number of the culture. another was enrichment of anti-dna producing cells at an early stage and prior to the cloning. the combination of these procedures allowed ready derivation of the cell lines secreting monoclonal anti-dna ... | 1984 | 6431009 |
| the role of b cell differentiation factors and specific t cell help in the pathogenesis of primary hypogammaglobulinemia. | we have examined the function of t and b cells from patients with late onset primary acquired hypogammaglobulinemia (phg). t cells from these patients give effective help to normal b cells for antigen-dependent antibody synthesis. phg mononuclear cells also synthesize normal quantities of b cell differentiation factors, which enhance igg, igm and antigen-dependent antibody synthesis by normal lymphocytes. while patient t cells appear to behave appropriately, the responsiveness of patient b cells ... | 1984 | 6437844 |
| a highly sensitive enzyme-linked immunosorbent assay to quantitate antibodies to epstein-barr virus membrane antigen gp340. | an enzyme-linked immunosorbent assay (elisa) has been developed for the detection of antibodies to epstein-barr (eb) virus membrane antigen (ma) glycoprotein, gp340, in tamarins. the assay was found to be a thousand-fold more sensitive than conventional indirect immunofluorescence tests and consequently it was possible to follow accurately the sequential production of specific antibodies to gp340 by tamarins during a course of immunization. | 1984 | 6440902 |
| t lymphocyte culture established by repeated stimulation with the autologous lymphoblastoid line. mhc class ii restricted interactions with b blasts. | an okt3+t4+t8-dr+ lymphocyte line was developed from an epstein-barr virus (ebv) seropositive individual by repeated stimulation in vitro with autologous ebv-infected b cells. the t cell population designated e-44 was carried for eight months in the presence of interleukin-2 and was repeatedly tested for cytotoxicity, proliferation and lymphokine production in response to the autologous and a panel of allogenic b cells. the e-44 cells lysed the autologous lymphoblastoid cell lines (lcl) and allo ... | 1984 | 6443579 |
| pathogenesis of rheumatoid arthritis. | rheumatoid arthritis (ra) is initiated by an unknown antigen(s) in the genetically programmed host. hla-dr4 is associated with ra. the antigen could be exogenous (e.g., epstein-barr virus, bacterial cell wall products) or endogenous (e.g., collagen or immunoglobulin). within the synovium, where the immune response begins, monocytes and lymphocytes are activated. polyclonal b-cell proliferation results, as well as production of monokines and lymphokines. the antibodies form immune complexes with ... | 1984 | 6420100 |
| malignant histiocytosis in the oropharynx. | malignant histiocytosis is characterized by systemic, progressive, and invasive proliferation of malignant histiocytes. the disorder is typically accompanied by fever, general fatigue, lymphadenopathy, and hepatosplenomegaly. a case of a 21-year-old woman with primary malignant histiocytosis of the oropharynx is reported. histologic diagnosis from the biopsy specimen was confused by infiltration of normal-appearing histiocytes and inflammatory cells. the titers of epstein-barr virus and the paul ... | 1984 | 6471863 |
| infectious mononucleosis during gestation: report of three women and their infants studied prospectively. | epstein-barr virus (ebv) infection during gestation has been associated retrospectively with the occurrence of fetal malformations. the referral in the first trimester of three women with infectious mononucleosis allowed us to study prospectively this question. infectious mononucleosis was confirmed by ebv-specific serology in two women and on the basis of the heterophil antibody response and atypical lymphocytosis in the third. the titers of ebv-specific antibodies in the cord sera of the infan ... | 1984 | 6473132 |
| determinants of immunological responsiveness in recurrent spontaneous abortion. | a defined group of 20 women suffering recurrent spontaneous abortions of unknown etiology was studied for determinants relevant to immune responsiveness. a low prevalence of serum anti-cmv antibodies (35%; cf, 65% in controls)--but not anti-herpes-simplex-virus or epstein-barr virus antibodies--was noted. only two (10%) of these women had lymphocytotoxic antibodies following their repeated pregnancies; low titer anti-sperm antibody was present in two other of these women. whereas there was no si ... | 1984 | 6474555 |
| insulin receptor degradation is accelerated in cultured lymphocytes from patients with genetic syndromes of extreme insulin resistance. | previous studies of the insulin receptor in disease states have utilized primarily techniques of equilibrium binding and, to a limited extent structural, analysis. though techniques have been developed to study receptor degradation in normal cells, they have not been applied to disease states. in the present study we have examined insulin receptor degradation rate in b lymphocytes that were obtained from peripheral blood of normal subjects and patients with several syndromes of extreme insulin r ... | 1984 | 6480829 |
| antibody-dependent cellular cytotoxicity. relation to stage and disease course in north american patients with nasopharyngeal carcinoma. | a prospective study of north american patients, mostly white, with different histopathologic types of nasopharyngeal carcinoma was initiated approximately five years ago. several anti-epstein-barr virus (ebv) serologic tests are being evaluated; one is the antibody-dependent cellular cytotoxicity (adcc) assay, which measures antibodies to an ebv-induced membrane antigen component. a low adcc titer at diagnosis reflects a poor prognosis, and the determination of antibody titers by this assay iden ... | 1984 | 6487126 |
| heterochromatin and nucleolus organizer regions in cells of patients with malignant and premalignant lymphatic diseases. | peripheral blood lymphocytes (short-term cultures) and permanent lymphoid cell lines (long-term cultures) of patients with hodgkin disease (5), lymphoma (1), plasma cell leukemia (1), angioimmunoblastic lymphadenopathy (1), and infectious mononucleosis (3) were investigated for c-band variants and nucleolus organizer region (nor)-activities by c-banding and silver-staining, and compared to those of healthy adults (5) and newborn children (3). heterochromatin polymorphisms were found in malignant ... | 1984 | 6490006 |
| treatment alternatives. head and neck cancers. | no tumor system is more amenable to multidiscipline therapy than head and neck cancers. diagnosis is enhanced by fine-needle cytology, computerized tomography (ct) scan, and tumor markers. modified surgical techniques, co2 laser, and immediate myocutaneous flap reconstruction have enhanced surgery. endocurie therapy, radiosensitizers, and particle therapy supplementation have enhanced radiotherapy. combination chemotherapy, immunotherapy, prostaglandin synthesis inhibitors, and retinoids have en ... | 1984 | 6498757 |
| complementation analysis in lymphoid cells from five patients with different forms of maple syrup urine disease. | the possibility of genetic heterogeneity in maple syrup urine disease was investigated by measuring branched-chain ketoacid dehydrogenase in polyethylene glycol-induced heterokaryons of lymphoid cells. the lymphoid cell lines from five patients with varying forms of the disease were established after incubation with epstein-barr virus. the results suggested that there are at least two genetic complementation groups in the disease. | 1984 | 6500555 |
| herpes simplex and herpes zoster. nervous system involvement. | mainly discussed are neurologic complications of two neurotropic herpesviruses: herpes simplex viruses types 1 and 2 and varicella-zoster virus. briefly discussed are cytomegalovirus and epstein-barr virus. treatment of immunosuppressed and nonimmunosuppressed patients is reviewed. | 1984 | 6503937 |
| proteins encoded by the human c-myc oncogene: differential expression in neoplastic cells. | to examine myc protein products in the wide variety of human tumor cells having alterations of the c-myc locus, we have prepared an antiserum against a synthetic peptide corresponding to the predicted c-terminal sequence of the human c-myc protein. this antiserum (anti-hu-myc 12c) specifically precipitated two proteins of 64 and 67 kilodaltons in quantities ranging from low levels in normal fibroblasts to 10-fold-higher levels in epstein-barr virus-immortalized and burkitt's lymphoma cell lines, ... | 1984 | 6513926 |
| use of cyclosporin a in establishing epstein-barr virus-transformed human lymphoblastoid cell lines. | we have investigated the potential for using cyclosporin a to increase the efficiency with which epstein-barr virus-transformed human lymphoblast lines can be prepared. use of this immunosuppressive drug has permitted the development of a procedure with success rates exceeding 95% despite the processing of very large numbers of samples. | 1984 | 6519667 |