Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| effects of bisphenol a-related diphenylalkanes on vitellogenin production in male carp (cyprinus carpio) hepatocytes and aromatase (cyp19) activity in human h295r adrenocortical carcinoma cells. | the present study investigated the effects of the known xenoestrogen bisphenol a (bpa) relative to eight bpa-related diphenylalkanes on estrogen receptor (er)-mediated vitellogenin (vtg) production in hepatocytes from male carp (cyprinus carpio), and on aromatase (cyp19) activity in the human adrenocortical h295r carcinoma cell line. of the eight diphenylalkanes, only 4,4'-(hexafluoropropylidene)diphenol (bhf) and 2,2'-bis(4-hydroxy-3-methylphenyl)propane (bpro) induced vtg, i.e., to a maximum o ... | 2005 | 15907334 |
| levels of organochlorine pesticides, polychlorinated biphenyls and polybrominated diphenyl ethers in fish species from kahramanmaras, turkey. | the levels of organohalogenated contaminants, such as organochlorine pesticides (ocps), polychlorinated biphenyls (pcbs) and polybrominated diphenyl ethers (pbdes) were measured in four fish species (acanthobrama marmid (kalashpa), cyprinus carpio (carp), chondrostoma regium (nose-carp), and silurus glanis (wels)) from the sir dam lake, kahramanmaras, turkey. these species were selected for their characteristic feeding behaviour and their importance to local human fish consumption. ddts were the ... | 2005 | 15910967 |
| koi herpesvirus represents a third cyprinid herpesvirus (cyhv-3) in the family herpesviridae. | the sequences of four complete genes were analysed in order to determine the relatedness of koi herpesvirus (khv) to three fish viruses in the family herpesviridae: carp pox herpesvirus (cyprinid herpesvirus 1, cyhv-1), haematopoietic necrosis herpesvirus of goldfish (cyprinid herpesvirus 2, cyhv-2) and channel catfish virus (ictalurid herpesvirus 1, ichv-1). the genes were predicted to encode a helicase, an intercapsomeric triplex protein, the dna polymerase and the major capsid protein. the re ... | 2005 | 15914843 |
| complementary dna cloning and constitutive expression of cytochrome p450 1c1 in the gills of carp (cyprinus carpio). | cytochrome p450 (cyp) enzymes constitute a multigene family of many endogenous and xenobiotic substances. the cyp1 family is of particular interest in environmental toxicology because its members are dominant in the metabolism of polycyclic aromatic hydrocarbons (pahs), polychlorinated biphenyls (pcbs), and aryl amines. a new complementary dna of the cyp1c subfamily encoding cyp1c1 was isolated from carp liver after intraperitoneal injection of beta-napthoflavone (bnf). the full-length cdna obta ... | 2005 | 15915151 |
| ontogeny of humoral immune parameters in fish. | the first appearance of igm in lymphocytes varies considerably among fish species. generally, the first appearance of b-lymphocytes and immunoglobulins is late in marine species compared to fresh water species, and larvae have reached about 20-30 mm in length when igm is first expressed. rainbow trout and channel catfish show the first appearances of surface igm at about 1 week after hatching. marine species like the sea bass, spotted wolffish and cod show igm positive lymphocytes 1-10 weeks aft ... | 2005 | 15916905 |
| cell proliferation and gill morphology in anoxic crucian carp. | is dna replication/cell proliferation in vertebrates possible during anoxia? the oxygen dependence of ribonucleotide reductase (rnr) could lead to a stop in dna synthesis, thereby making anoxic dna replication impossible. we have studied this question in an anoxia-tolerant vertebrate, the crucian carp (carassius carassius), by examining 5'-bromo-2'-deoxyuridine incorporation and proliferating cell nuclear antigen levels in the gills, intestinal crypts, and liver. we exposed crucian carp to 1 and ... | 2005 | 15919732 |
| extracellular calcium is required for the maintenance of plasma membrane integrity in nucleated cells. | in contrast to rat and human erythrocytes, nucleated erythrocytes from two fish species (cyprinus carpio and salmo trutta) underwent almost complete haemolysis in 20 min of edta addition. using ca2+/mg2+ egta-citrate buffer, we observed that half-maximal haemolysis of fish erythrocytes occurs at [ca2+]o approximately 10 microm independently of extracellular mg2+ concentration. attenuation of [ca2+]o with egta also decreased stability of the plasma membrane of vascular smooth muscle cells (vsmc) ... | 2005 | 15936814 |
| improvement of nutritive value of grass pea (lathyrus sativus) seed meal in the formulated diets for rohu, labeo rohita (hamilton) fingerlings after fermentation with a fish gut bacterium. | eight isonitrogenous (35% crude protein approximately) and isocaloric (4.0 kcalg(-1) approximately) diets were formulated incorporating raw and fermented grass pea (lathyrus sativus) seed meal at 10%, 20%, 30% and 40% levels by weight into a fish meal based diet and fed to rohu, labeo rohita, fingerlings for 80 days and fish performance was studied. a particular bacterial strain (bacillus sp.) isolated from the intestine of adult common carp (cyprinus carpio) reared in the wild having significan ... | 2005 | 15939274 |
| assessment of heavy metal concentrations in the food web of lake beyşehir, turkey. | the accumulation of heavy metals (cd, pb, hg and cr) was measured by atomic absorption spectrophotometry in water, sediment, plankton and fish samples collected from lake beyşehir, which is important bird nesting and visiting areas, and irrigation and drinking water sources. in lake beyşehir, the accumulation orders of heavy metals were cd>pb>cr>hg in water, pb>cd>cr>hg in sediment, pb>cd>cr>hg in plankton, and cd>pb>cr>hg in the muscles and gills of chub, carp, tench, except for the muscle of p ... | 2005 | 15950047 |
| constitutive expression of three novel catfish cxc chemokines: homeostatic chemokines in teleost fish. | chemokines are best known for their vital role in leukocyte chemotaxis, as part of the larger inflammatory response. expression analysis and functional characterization of chemokines in mammalian species have often overlooked the role of these proteins under homeostatic conditions. recent investigations of chemokine diversity in teleost fish have also centered on the immune-related functions of chemotactic cytokines, such as cxcl8 and cxcl10. while a disease-based approach to chemokines is essen ... | 2005 | 15950731 |
| congenital heart disease reminiscent of partial trisomy 2p syndrome in mice transgenic for the transcription factor lbh. | partial trisomy 2p syndrome includes a spectrum of congenital heart disease (chd) that is characterized by complex malformations of the outflow and inflow tracts, defects in cardiac septation, heart position, as well as abnormal ventricular development. lbh (limb-bud and heart) is a novel, highly conserved putative transcriptional regulatory protein, which displays a unique spatiotemporal gene expression pattern during early mouse heart development. here we show that human lbh maps to chromosome ... | 2005 | 15958514 |
| development of a cost-effective oral vaccination method against viral disease in fish. | different vaccination methods have been applied to protect fish against the detrimental effects of various pathogens. several studies have shown the potentials of oral vaccination. in theory oral vaccination is an effortless and stress-free method which can be applied at almost any age. in general, however, the vaccine has to be protected to avoid digestion, which results in high costs for application in aquaculture. in this paper we introduce a cost-effective oral vaccination strategy for viral ... | 2005 | 15962477 |
| fish rhabdoviruses: molecular epidemiology and evolution. | rhabdoviruses may cause serious diseases in wild and farmed fish. within the rhabdoviridae six genera have been established: ephemerovirus, cytorhabdovirus, nucleorhabdovirus, lyssavirus, vesiculovirus, and novirhabdovirus. viruses that infect fish are official or tentative members of the genera vesiculovirus and novirhabdovirus, or are listed as unassigned rhabdoviruses. in this report, we summarize and discuss published and our own unpublished data on the molecular epidemiology and phylogeogra ... | 2005 | 15981469 |
| characterization of transgene integration pattern in f4 hgh-transgenic common carp (cyprinus carpio l.). | the integration pattern and adjacent host sequences of the inserted pmthgh-transgene in the f4 hgh-transgenic common carp were extensively studied. here we show that each f4 transgenic fish contained about 200 copies of the pmthgh-transgene and the transgenes were integrated into the host genome generally with concatemers in a head-to-tail arrangement at 4-5 insertion sites. by using a method of plasmid rescue, four hundred copies of transgenes from two individuals of f4 transgenic fish, a and b ... | 2005 | 15987603 |
| a survey of potential stressor-induced physiological changes in carp (cyprinus carpio) and barbel (barbus bocagei) along the tajo river. | the objective of this study was to evaluate fish response to acute stress induced by confinement after capture. because of the previously reported presence of chemical compounds in the tajo river basin where the study samplinig took place, an exposure biomarker to organic chemicals (retinol) was used. cortisol and glucose were used as stress biomarkers. plasma levels of cholesterol were used as lipidic metabolism indicators, and retinol level was used as a specific exposure biomarker. a referenc ... | 2005 | 15793831 |
| calcium-permeable ampa receptor plasticity is mediated by subunit-specific interactions with pick1 and nsf. | a recently described form of synaptic plasticity results in dynamic changes in the calcium permeability of synaptic ampa receptors. since the ampa receptor glur2 subunit confers calcium permeability, this plasticity is thought to occur through the dynamic exchange of synaptic glur2-lacking and glur2-containing receptors. to investigate the molecular mechanisms underlying this calcium-permeable ampa receptor plasticity (carp), we examined whether ampa receptor exchange was mediated by subunit-spe ... | 2005 | 15797551 |
| disruption of the gene euchromatin histone methyl transferase1 (eu-hmtase1) is associated with the 9q34 subtelomeric deletion syndrome. | a new syndrome has been recognised following thorough analysis of patients with a terminal submicroscopic subtelomeric deletion of chromosome 9q. these have in common severe mental retardation, hypotonia, brachycephaly, flat face with hypertelorism, synophrys, anteverted nares, thickened lower lip, carp mouth with macroglossia, and conotruncal heart defects. the minimum critical region responsible for this 9q subtelomeric deletion syndrome (9q-) is approximately 1.2 mb and encompasses at least 1 ... | 2005 | 15805155 |
| identification of the genes involved in enhanced fenretinide-induced apoptosis by parthenolide in human hepatoma cells. | fenretinide (n-4-hydroxyphenyl retinamide, 4hpr) is a synthetic anticancer retinoid that is a well-known apoptosis-inducing agent. recently, we observed that the apoptosis induced by fenretinide could be effectively enhanced in hepatoma cells by a concomitant treatment with parthenolide, which is a known inhibitor of nuclear factor-kappab (nf-kappab). furthermore, treatment with fenretinide triggered the activation of nf-kappab during apoptosis, which could be substantially inhibited by partheno ... | 2005 | 15805281 |
| expression profiling with progression of dystrophic change in dysferlin-deficient mice (sjl). | the sjl mouse is a model for human dysferlinopathy (limb-girdle muscular dystrophy type 2b and miyoshi myopathy). we used cdna microarrays to compare the expression profiles of 10,012 genes in control and sjl quadriceps femoris muscles in order to find genes involved in the degeneration and regeneration process and in dysferlin's functional network. many genes involved in the process of muscle regeneration are observed to be up-regulated in sjl mice, including cardiac ankyrin repeated protein (c ... | 2005 | 15811552 |
| caspase-dependent processing activates the proapoptotic activity of deleted in breast cancer-1 during tumor necrosis factor-alpha-mediated death signaling. | deleted in breast cancer-1 (dbc-1) was initially cloned from a homozygously deleted region in breast and other cancers on human chromosome 8p21, although no function is known for the protein product it encodes. we identified the generation of amino-terminally truncated versions of dbc-1 during tumor necrosis factor (tnf)-alpha-mediated apoptosis. full-length 150 kda dbc-1 underwent caspase-dependent processing during tnf-alpha-mediated death signaling, to produce p120 dbc-1 and p66 dbc-1 carboxy ... | 2005 | 15824730 |
| involvement of gadd153 and cardiac ankyrin repeat protein in hypoxia-induced apoptosis of h9c2 cells. | oxidative stress is the main cause of cardiac injury during ischemia/reperfusion but the molecular mechanism for this process is unclear. in this study, it was found that hypoxia induces apoptosis in rat embryonic heart-derived h9c2 cells leading to the induction of gadd153, which is an apoptosis-related gene. therefore, this study addressed the molecular role of gadd153 in hypoxia-induced apoptosis. the stable or inducible overexpression of gadd153 sensitized the h9c2 cells to apoptotic cell de ... | 2005 | 15826945 |
| temperature sensitivity of calcium binding for parvalbumins from antarctic and temperate zone teleost fishes. | parvalbumin (pv) is a soluble calcium-binding protein that is especially abundant in fast-twitch muscles of fish and other lower vertebrates. despite its prevalence in ectothermic taxa, few data address the effects of temperature on pv binding function. in this study, calcium dissociation constants (kd) were measured as a function of temperature (0-25 degrees c) for pv from two antarctic (gobionotothen gibberifrons and chaenocephalus aceratus) and two temperate zone fish species (cyprinus carpio ... | 2005 | 15748857 |
| determination of select antidepressants in fish from an effluent-dominated stream. | increasing evidence indicates widespread occurrence of pharmaceuticals and personal care products (ppcps) in municipal effluent discharges and surface waters. studies that characterize the fate and effects of ppcps in aquatic systems are limited, and to our knowledge, data regarding pharmaceutical accumulation in fish of effluent-dominated ecosystems have not been previously reported. in the present study, fish populations were sampled from a reference stream and an effluent-dominated stream in ... | 2005 | 15720009 |
| ultrasonographic findings of facial dysmorphism in wolf-hirschhorn syndrome. | wolf-hirschhorn syndrome has characteristic craniofacial dysmorphism with severe fetal growth restriction. we present a case that revealed facial dysmorphism characteristic of wolf-hirschhorn syndrome with two- and three-dimensional ultrasonography. the facial dysmorphic features in the present case were dolichocephaly; high forehead; large rectangular nose continuing to the eyebrows; hypertelorism; short philtrum; carp-shaped mouth; microretrognathia; and large, simply modeled, low-set ears. ch ... | 2005 | 15731989 |
| [experimental studies on the central analgesic effect of the non-steroidal anti-inflammatory drug carprofen in a sheep model -- preliminary results]. | to evaluate the effect of the non-steroidal anti-inflammtory drug carprofen on the lumbar cerebrospinal fluid (csf) concentration of noradrenaline (na) and serotonin (5-ht) in non-stimulated (part i) and surgically stimulated (part ii) sheep. | 2005 | 15645384 |
| [studies on liquid fermentation of anamorph of shiraia bambusicola]. | a asexuae strain producing effective component hypocrellin was isolated from the hypha of shiraia bambusicola sporo-carp. by liquid-state fermentation and orthogonal teat with shake flask culture, the optimum medium component and culture conditions were: saccharose 20 g/l, yeast extract 8 g/l, maize extract 2 g/l, ph 6.0, culture broth quantity 50/250 ml(v/v) flask, inoculum 10% (v/v), culture temperature 28 degrees c, shake rotation 130 r/min, fermentation period 96 h. the culture can produce 8 ... | 2005 | 16568656 |
| acute dysphagia after carp fish meal: a case report. | an 80-year-old man was admitted to the emergency department with acute dysphagia symptoms of 8 hours' duration. the problem began after a carp fish meal. prominent symptoms included inability to swallow, mild dyspnea, and cough. laboratory evaluation and fiber-optic examination of the upper aerodigestive tract was normal except for accumulation of saliva in the hypopharynx. examination with a flexible esophagoscope revealed a total blockage of the lumen of the lower esophagus due to impaction of ... | 2005 | 16369319 |
| acute effects of hexavalent chromium on survival, oxygen consumption, hematological parameters and some biochemical profiles of the indian major carp, labeo rohita. | an attempt has been made in the present investigation to determine the acute toxicity of hexavalent chromium and its toxicological effects on survival, physiological, hematological and biochemical parameters of the widely consumed indian major carp, labeo rohita. short-term acute toxicity tests were performed adopting renewal bioassay technique (usepa, 1975) over a period of 96 h, using different concentrations of potassium dichromate to the fish and the 96 h lc[50] value was found to be 111.45 ... | 2005 | 16819101 |
| detection of carp interstitial nephritis and gill necrosis virus in fish droppings. | carp interstitial nephritis and gill necrosis virus (cngv) is an unclassified large dna virus that morphologically resembles members of the herpesviridae but contains a large (ca. approximately 280-kbp) linear double-stranded dna. this virus has also been named koi herpesvirus, koi herpes-like virus, and cyprinid herpesvirus 3. cngv is the cause of a lethal disease that afflicts common carp and koi. by using immunohistochemistry, molecular analysis, and electron microscopy we previously demonstr ... | 2005 | 16269770 |
| arsenic speciation in farmed hungarian freshwater fish. | arsenic speciation analysis was carried out on freshwater farmed fish collected from an area with elevated groundwater arsenic concentrations in hungary as well as from outside of the area (control samples). the arsenic species were determined by high-performance liquid chromatography-inductively coupled plasma mass spectrometry on methanol extracts of the muscle tissue from the fish. catfish (claries gariepinus) were raised in geothermal water where the average total arsenic concentrations were ... | 2005 | 16277428 |
| algorithms for selecting informative marker panels for population assignment. | given a set of potential source populations, genotypes of an individual of unknown origin at a collection of markers can be used to predict the correct source population of the individual. for improved efficiency, informative markers can be chosen from a larger set of markers to maximize the accuracy of this prediction. however, selecting the loci that are individually most informative does not necessarily produce the optimal panel. here, using genotypes from eight species--carp, cat, chicken, d ... | 2005 | 16305328 |
| [koi herpesvirus disease]. | koi herpesvirus (khv) disease emerged at the late 1990s, and has rapidly spread to the world. in japan, khv disease first occurred at october 2003. the disease resulted in mass mortality of wild carp as well as cultured carp. until now, khv-infected carp were found in 42 out of 47 prefectures in japan. only carp cyprinus carpio is susceptible to khv, while goldfish, closely-related species to carp, is not. the affected carp swim lethargically. sunken eyes and gill necrosis are frequently noticed ... | 2005 | 16308541 |
| distribution of persistent organochlorine pesticides in tissue/organ of silver carp (hypophthalmichthys molitrix) from guanting reservoir, china. | the concentration of organochlorine pesticides (ocps) in tissues and organs of silver carp (hypophthalmichthys molitrix) from guanting reservoir were investigated to evaluate the pollution potential and distribution of ocps. a total of 16 ocps were measured and the concentrations were in the range of 1.61-69.01 ng/g wet weight (ww) for total ocps, 0.16-0.75 ng/g ww for. hcb, 0.75-26.80 ng/g ww for sigmahch (sum of alpha-, beta-, gamma- and delta-hch) and 0.68-35.94 ng/g ww for sigmaddt( sum of p ... | 2005 | 16312991 |
| structure-activity relationship of neuropeptide gamma derived from mammalian and fish. | this study of relationship between structure and biologic activity was performed using five neuropeptide gammas [npgamma; mammalian-npgamma (m-npgamma), trout-npgamma (t-npgamma), goldfish-npgamma (g-npgamma), bowfin-npgamma (b-npgamma), and shark-npgamma (s-npgamma)]. circular dichroism (cd) spectra showed that all peptides took random structure in buffer solution. in neutral and acidic liposomes, m-npgamma, t-npgamma, b-npgamma, and s-npgamma still adopted random structure, while g-npgamma had ... | 2005 | 16316456 |
| the tapeworm ligula intestinalis (cestoda: pseudophyllidea) inhibits lh expression and puberty in its teleost host, rutilus rutilus. | the tapeworm ligula intestinalis occurs in the body cavity of its cyprinid second intermediate host, in this study the roach rutilus rutilus, and inhibits host gonadal development. the mechanism by which infected fish are prevented from reproducing is unknown. comparison of parameters, such as body length and weight, and condition factor and age, between infected and uninfected individuals, indicated only minor effects of parasitism on growth and condition. in contrast, seasonal gonadal developm ... | 2005 | 16322554 |
| seasonal ovarian cycle in freshwater teleost, labeo rohita (ham.) in tarai region of uttaranchal. | seasonal ovarian cycle, based on histophysiological changes, and correlative variations in liver were studied in an indian major carp, labeo rohita reared in captivity under ambient environmental conditions in tarai region of uttaranchal. the oocyte features used for marking ovarian cycle included nuclear-ooplasmic ratio, yolk vesicle incorporation into ooplasm, number and size of nucleolus and differentiation of follicular layer. based on these features the ovarian cycle in l. rohita was divide ... | 2005 | 16334297 |
| use of a probiotic to control lactococcosis and streptococcosis in rainbow trout, oncorhynchus mykiss (walbaum). | from a comparison of 125 bacterial isolates recovered from the digestive tract of rainbow trout, oncorhynchus mykiss, and carp, cyprinus sp., a culture was obtained which was effective at preventing clinical disease caused by lactococcus garvieae and streptococcus iniae when used as a feed additive. the culture, aeromonas sobria gc2, was incorporated into the feed and fed to rainbow trout (average weight = 20 g) for 14 days at a dose equivalent to 5 x 10(7) cells g(-1) of feed. whereas the untre ... | 2005 | 16336470 |
| fish genomics and biology. | the last common ancestor between fish and mammals dates back to the very origin of the vertebrate lineage and today, half of modern vertebrates are fish. it is thus not surprising that several fish species have played important roles in recent years to advance our understanding of vertebrate genome evolution, to inform us on the structure of human genes, and, somewhat more unexpectedly, to provide leads to understanding the function of genes involved in human diseases. genome sequence comparison ... | 2005 | 16339365 |
| reactivation of koi herpesvirus infections in common carp cyprinus carpio. | two co-habitation studies with common carp were conducted to determine whether latent infections of koi herpesvirus (khv) exist. fish were exposed to khv using 2 different temperature profiles, which induced low and high initial mortality. subsequently, certain groups of fish were co-habited with naive fish while others were not. koi herpesvirus was reactivated in fish from 3 of the 5 experimental tanks. reactivation of the virus occurred regardless of the initial mortality associated with the v ... | 2005 | 16385803 |
| population dynamics of lernaea cyprinacea (crustacea: copepoda) on four cyprinid species. | a total of 217 barbus graellsii, 54 barbus haasi, 417 cyprinus carpio and 85 leuciscus cephalus captured at 9 sampling stations in the central basin of the llobregat river (ne spain) were studied for lernaea cyprinacea parasitism. prevalence (b. graellsii 39.2, b. haasi 31.5, c. carpio 8.4, l. cephalus 48.2), intensity of infection (b. graellsii range 1 to 39, mean 4.6; b. haasi range 1 to 7, mean 2.6; c. carpio range 1 to 16, mean 3.1; l. cephalus range 1 to 8, mean 2.4), and abundance (b. grae ... | 2005 | 16385816 |
| isolation and characterization of visual pigment kinase-related genes in carp retina: polyphyly in grk1 subtypes, grk1a and 1b. | visual pigment is phosphorylated and inactivated after light stimulus. the responsible enzyme is known as rhodopsin kinase or g-protein-coupled receptor kinase 1 (grk1) in rods. we recently showed that the kinase in cones (grk7) has much higher activity than grk1 in rods in carp retina. during the course of these studies, we realized that there are several subtypes of grk1 and grk7. in the present study, therefore, to identify the grk1 and grk7 subtypes expressed in carp photoreceptors, we deter ... | 2005 | 16402022 |
| survival of haplorchis taichui metacercariae in lab-pla, thai traditional food preparation. | lab-pla is a famous kind of thai traditional food in northern and northeasthern thailand. consumption of uncooked lab-pla can lead to trematode infection because cyprinoid fish serve as the 2nd intermediate hosts of trematodes. the preparation of lab-pla can be made in different ways, depending on seasonings used in different places. the effect of the seasonings used in lab-pla on the survival of haplorchis taichui metacercariae were, therefore, determined. the methodology was done by the follow ... | 2005 | 16438192 |
| [seasonal distribution of gill parasites in fishes from fee-fishing at guariba, são paulo state, brazil]. | this study was accomplished with the objective of knowing the seasonal distribution of the main species of parasites causes of branchial damages to the fish from fee-fishing at guariba, state of são paulo. this city is situated at 21 degrees 15'22'' s, 48 degrees 18'58'' w, in altitude 595 meters. this research was conducted between april, 1997 to march, 1999. from 408 fishes examined, 29.1% of them were infested by the protozoans e metazoans. leporinus macrocephalus showed the highest parasitis ... | 2005 | 16445870 |
| dimerization of the cardiac ankyrin protein carp: implications for marp titin-based signaling. | cardiac ankyrin repeat protein (carp) and its two close homologs ankrd2 (arpp) and darp correspond to a conserved gene family of muscle ankyrin repeat proteins (marps). all three genes respond to a variety of stress/strain injury signals with their cytokine-like induction and can associate with the elastic region of titin/connectin. recently, both carp and ankrd2 were observed to be elevated in cardiac diseases as well as muscular dystrophies, implicating their joined signaling in muscle disease ... | 2005 | 16450059 |
| [an investigation of metazoan parasites of common carp (cyprinus carpio l.) in lake eber, afyon, turkey.]. | in this study, metazoan parasites of the following fish species were investigated in lake eber (afyon) from july 2002-2003. at the end of this research, 2 species of monogenea (gyrodactylus elegans, dactylogyrus extensus) were found on the gills and fins of the fishes; 1 species of digenea (posthodiplostomum cuticola), on skin and fins; 1 species of cestoda (bothriocephalus acheilognathi) in the intestines; and 1 species of arthropoda (argulus foliaceus), the skin, gills and fins of fish. the di ... | 2005 | 17160824 |
| description of an as yet unclassified dna virus from diseased cyprinus carpio species. | numerous deaths of koi and common carp (cyprinus carpio) were observed on many farms throughout israel, resulting in severe financial losses. the lethal viral disease observed is highly contagious and extremely virulent, but morbidity and mortality are restricted to koi and common carp populations. diseased fish exhibit fatigue and gasping movements in shallow water. infected fish had interstitial nephritis and gill necrosis as well as petechial hemorrhages in the liver and other symptoms that w ... | 2005 | 15681400 |
| molecular cloning of leukocyte cell-derived chemotaxin 2 in rainbow trout. | in humans, leukocyte cell-derived chemotaxin 2 (lect2) is a 16kda chemotactic protein that consists of 133 amino acids and three intramolecular disulphide bonds. although it was originally demonstrated to have a chemotactic function in vitro, recent data sustain a further multifunctional role of lect2 that extends from cell growth, differentiation, damage/repair process and carcinogenesis to autoimmune diseases. the in vivo function of lect2 protein still remains obscure. in order to study the p ... | 2005 | 15683915 |
| development and evaluation of an experimental model of cutaneous columnaris disease in koi cyprinus carpio. | a reproducible, experimental model of columnaris disease was developed to study the pathogenesis of cutaneous disease associated with flavobacterium columnare infection in koi (cyprinus carpio). in experimental infections, lesions were usually restricted to skin and fins; gill necrosis was not a consistent finding. cytologic and histopathologic examinations provided a presumptive diagnosis of columnaris disease. specific detection of f. columnare was done using the polymerase chain reaction and ... | 2005 | 15690950 |
| catalytic and structural characteristics of carp hepatopancreas d-amino acid oxidase expressed in escherichia coli. | d-amino acid oxidase of carp (cyprinus carpio) hepatopancreas was overexpressed in escherichia coli cells and purified to homogeneity for the first time in animal tissues other than pig kidney. the purified preparation had a specific activity of 293 units mg(-1) protein toward d-alanine as a substrate. it showed the highest activity toward d-alanine with a low km of 0.23 mm and a high kcat of 190 s(-1) compared to 10 s(-1) of the pig kidney enzyme. nonpolar and polar uncharged d-amino acids were ... | 2005 | 15694590 |
| ankrd1 specifically binds casq2 in heart extracts and both proteins are co-enriched in piglet cardiac purkinje cells. | it has been suggested that the cardiac ankyrin repeat domain 1 protein (ankrd1), also known as carp, can play a pathophysiological role in the contractile responsiveness of myocardium. here, we study the potential functional roles of ankrd1 by searching for endogenous cardiac proteins that interact preferentially with ankrd1 in the heart-tissue extract from neonatal piglets, using non-biased pull-down approaches. these approaches identified, for the first time, a selective interaction between an ... | 2005 | 15698842 |
| epitheliocystis in carp (cyprinus carpio) in south korea. | epitheliocystis in the carp of a pet fish market were investigated by our diagnostic work and collecting information from department of laboratory animal medicine and fish & shellfish laboratory. the epitheliocystis was identified by using histopathological examination. epitheliocystis was confirmed as inflammation, epithelial hyperplasia, and lamellar fusion of the gill tissue. electron microscopic observation showed that the inclusions were filled with chlamydia-like organism. | 2005 | 15699608 |
| novel immunoglobulin-like transcripts in teleost fish encode polymorphic receptors with cytoplasmic itam or itim and a new structural ig domain similar to the natural cytotoxicity receptor nkp44. | members of the immunoglobulin superfamily (igsf) include a group of innate immune receptors located in the leukocyte receptor complex (lrc) and other small clusters such as the trem/nkp44 cluster. these receptors are characterised by the presence of immunoglobulin domains, a stalk, a transmembrane domain, and a cytoplasmic region containing either an immunoreceptor tyrosine-based inhibitory motif (itim) or are linked to an adapter molecule with an activation motif (itam) for downstream signallin ... | 2005 | 15702329 |
| the life cycle of henneguya nuesslini schuberg & schroder, 1905 (myxozoa) involves a triactinomyxon-type actinospore. | the life cycle of the histozoic myxozoan parasite henneguya nuesslini was investigated in two salmonid host species. naive brown trout, salmo trutta, and brook trout, salvelinus fontinalis, were experimentally infected in two trials by triactinomyxon type actinospores from naturally infected tubifex tubifex. in exposed common carp, cyprinus carpio, no myxospore production was detected. the parasite formed cysts with mature myxospores in the connective tissue of the fish 102 days post-exposure. t ... | 2005 | 15705152 |
| genetic relationships among members of the ichthyobodo necator complex: implications for the management of aquaculture stocks. | abstract ichthyobodo necator (costia) is a common and important flagellate parasite that infests the skin and gills of many freshwater and marine fish. costia infestations are often fatal and cause significant aquaculture losses worldwide. recently it has been demonstrated that ichthyobodo is a multispecies complex with differing host preferences. knowing if those species have broad or narrow host specificity has important implications for the management of costia. to address the question of hos ... | 2005 | 15705156 |
| molecular cloning of prepro-thyrotropin-releasing hormone cdnas from the common carp cyprinus carpio and goldfish carassius auratus. | to expand our knowledge on the evolution of prepro-thyrotropin-releasing hormone (pptrh) from fish to tetrapods, sequences of pptrh cdnas from two cyprinid teleosts, the common carp cyprinus carpio and goldfish carassius auratus, were determined. degenerate primers were designed based on the conserved regions between the zebrafish pptrh sequence identified from the zebrafish est database and the sockeye salmon pptrh sequence, and pcr amplification was performed. full-length pptrhs were confirmed ... | 2005 | 15707606 |
| carp liver dnase--isolation, further characterization and interaction with endogenous actin. | deoxyribonuclease i (dnase i)-like enzyme from the liver of the carp (cyprinus carpio) was purified to homogeneity and further characterized. ion exchange chromatography on deae-cellulose, molecular filtration on sephacryl s-300 and con a-sepharose affinity chromatography were applied for enzyme isolation. carp liver dnase, similarly to dnase i from bovine pancreas, was found to be an endonuclease that hydrolyses linear dna from salmon sperm as well as circular dna forms--plasmid and cosmid. the ... | 2005 | 15621519 |
| carp, a cardiac ankyrin repeat protein, is up-regulated during wound healing and induces angiogenesis in experimental granulation tissue. | cardiac ankyrin repeat protein (carp) was identified by subtractive hybridization as one of a group of genes that are rapidly modulated by acute wounding of mouse skin. quantitative rt-pcr showed that carp was strongly induced during the first day after wounding (157.1-fold), and the high level persisted for up to 14 days. immunohistochemistry and in situ hybridization revealed that carp was expressed in skeletal muscle, vessel wall, hair follicle, inflammatory cells, and epidermis in the wound ... | 2005 | 15632022 |
| dna aggregating protein from carp (cyprinus capio) eggs. | hemagglutinating activity for glutaraldehyde-fixed, trypsinized human and rabbit erythrocytes was obtained from carp (ciprinus capio) egg extract. this activity was inhibited by sulfated glycans such as heparin and fucoidan, and salmon testis dna (stdna), but not by mono- and oligosaccharides. the active fraction from hydroxyapatite column, ha400, caused aggregation of stdna at a concentration of 125 microg/ml. ha400-induced aggregation was inhibited by heparin and fucoidan at the concentrations ... | 2005 | 15635155 |
| stimulation of immunity in indian major carp catla catla with herbal feed ingredients. | catla catla, catla (150 +/- 20 g) were fed a diet containing seed of achyranthes aspera (0.5%) and control diet without a. aspera for four weeks prior to and after ip injection with chicken erythrocytes. fish were sampled for four consecutive weeks after immunization. hemagglutination antibody titers were significantly higher in the test group of fishes compared with the control group. serum globulin levels were significantly (p(t-test) < 0.05) higher in the test group than control group on days ... | 2005 | 15561562 |
| complete nucleotide sequence of the hirame rhabdovirus, a pathogen of marine fish. | reverse transcription-polymerase chain reaction (rt-pcr) derived clones were constructed for the hirame rhabdovirus (hirrv) ca-9703 strain from korea, and the dna was sequenced. the 3'-end of genomic rna was cloned by poly(a)-tailing of the genomic rna before reverse transcription, and the 5'-end of the genome was cloned by poly(g)- or poly(c)-tailing of the first strand, followed by pcr. the remainder of the genomic dna was cloned by reverse transcription-polymerase chain reaction using primers ... | 2005 | 15567027 |
| protection against experimental aeromonas salmonicida infection in carp by oral immunisation with bacterial antigen entrapped liposomes. | liposome-entrapped atypical aeromonas salmonicida antigen was prepared to investigate the potential protective efficacy for a. salmonicida infection. carp (cyprinus carpio) were immunised orally with liposome-entrapped a. salmonicida antigen. after immunisation, significantly higher antigen-specific antibodies were detected in serum, intestinal mucus and bile than non-immunised control group. furthermore, immunised carp were challenged by immersion with 1 x 10(6) cfu ml(-1) of a. salmonicida for ... | 2005 | 15519542 |
| carp (cyprinus carpio l.) innate immune factors are present before hatching. | expression of the innate immune factors, complement factor 3 (c3), alpha2-macroglobulin (alpha2m), serum amyloid a (saa) and a complement factor 1 r/s--mannose binding lectin associated serine protease-like molecule (c1/masp2), was determined with real time quantitative-pcr in carp (cyprinus carpio l.) ontogeny around hatching. furthermore, the expression of c3 mrna and the presence of c3 protein were studied in carp embryos and larvae using in situ hybridisation, western blotting and immunohist ... | 2006 | 16169251 |
| swimming efficiency and the influence of morphology on swimming costs in fishes. | swimming performance is considered a main character determining survival in many aquatic animals. body morphology highly influences the energetic costs and efficiency of swimming and sets general limits on a species capacity to use habitats and foods. for two cyprinid fishes with different morphological characteristics, carp (cyprinus carpio l.) and roach (rutilus rutilus (l.)), optimum swimming speeds (u(mc)) as well as total and net costs of transport (cot, ncot) were determined to evaluate di ... | 2006 | 16177894 |
| comparative evaluation of the alkaline comet assay with the micronucleus test for genotoxicity monitoring using aquatic organisms. | a comparative analysis between the in vivo comet assay and the in vivo micronucleus test (mnt) was carried out in three aquatic organisms suitable for genotoxicity monitoring, carp (cyprinus carpio), rainbow trout (oncorhynchus mykiss), and clam (spisula sachalinensis), using a direct-acting mutagen, n-methyl-n'-nitro-n-nitrosoguanidine (mnng), and an indirect mutagen, benzo[a]pyrene (b[a]p). by optimizing the conditions for cell isolation, gill and liver (or digestive glands) were selected as t ... | 2006 | 16026836 |
| in vivo evoked specific cell mediated cytotoxicity in carp (cyprinus carpio l.) uses mainly a perforin/granzyme-like pathway. | a specific cytotoxicity assay has been developed for carp using the epithelioma papulosum cyprini (epc) cell line as target cells and the release of lactate dehydrogenase (ldh) as read out system. non-specific cytotoxicity against epc was not observed, but animals immunized with epc clearly showed specific killing by effector cells present in kidney, blood and spleen. this killing was strongly calcium dependent, suggesting the utilization of a perforin/granzyme-like pathway. however, blocking wi ... | 2006 | 15916904 |
| comparison of evolutionary rates in the mitochondrial dna cytochrome b gene and control region and their implications for phylogeny of the cobitoidea (teleostei: cypriniformes). | it is widely accepted that mitochondrial dna (mtdna) control region evolves faster than protein encoding genes with few exceptions. in the present study, we sequenced the mitochondrial cytochrome b gene (cyt b) and control region (cr) and compared their rates in 93 specimens representing 67 species of loaches and some related taxa in the cobitoidea (order cypriniformes). the results showed that sequence divergences of the cr were broadly higher than those of the cyt b (about 1.83 times). however ... | 2006 | 16213167 |
| characterization of serum immunoglobulin m of grouper and cdna cloning of its heavy chain. | immunoglobulin m (igm) from the whole serum of grouper fish, epinephelus coioides was purified by affinity chromatography using protein a-sepharose column. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) under reducing conditions revealed that the relative molecular masses (mr) of the equimolar heavy and light chains of igm were 78,000 and 27,000, respectively. the cdnas encoding igm heavy chain comprising its variable (vh) and constant (ch) regions have been cloned and sequ ... | 2006 | 16199094 |
| the in vitro effects of cpg oligodeoxynucleotides on the expression of cytokine genes in the common carp (cyprinus carpio l.) head kidney cells. | synthetic oligodeoxynucleotides with cpg motifs (cpg-odns) have gained attention because it elicits strong innate immune responses. cpg-odns promote the production of t-helper 1(t(h)1) and pro-inflammatory cytokines. in fish, knowledge of the effects of cpg-odns on the expression of cytokine genes is scarce. in this study, we report that cpg-odns induce the expression of pro-inflammatory cytokines in common carp head kidney leucocytes in vitro. evidence of a t(h)1 type immune stimulation was obs ... | 2006 | 16229898 |
| quantitative structure-toxicity relationships of organophosphorous pesticides to fish (cyprinus carpio). | this study was conducted to determine the relationships between 1381 chemical and structural parameters of 43 organophosphorus pesticides (ops) and their toxicity to fish, cyprinus carpio, using chemoffice 8.03 and dragon 2.1. by multivariate linear regression and intervariable regression analyses, various equations have been derived to calculate the lethal toxicity value, lc(50), for 43 ops found in fish with different levels of toxicity. results show that for all selected ops, especially those ... | 2006 | 16246398 |
| possible implication of macrophages in the regulation of cytochrome p450 activities in carp (cyprinus carpio). | macrophages play a key role in the regulation of cytochrome p450 activity induced by immunostimulants in mammals. we investigated the effects of immunostimulants (lps, dextran sulfate and tilorone) on biotransformation and macrophage activities in carp. the major effect of lps was its capacity to inhibit 3-mc-induced cytochrome p450 activities in the liver and head kidney. basal phase i activities were reduced by tilorone and dextran sulfate in immune organs. tilorone and dextran sulfate differe ... | 2006 | 16337134 |
| evidence for recombination of mitochondrial dna in triploid crucian carp. | in this study, we report the complete mitochondrial dna (mtdna) sequences of the allotetraploid and triploid crucian carp and compare the complete mtdna sequences between the triploid crucian carp and its female parent japanese crucian carp and between the triploid crucian carp and its male parent allotetraploid. our results indicate that the complete mtdna nucleotide identity (98%) between the triploid crucian carp and its male parent allotetraploid was higher than that (93%) between the triplo ... | 2006 | 16322508 |
| autoradiographic localization and binding study of benzodiazepines receptor sites in carp brain (cyprinus carpio l.). | this study demonstrates, for the first time, by both autoradiography and binding assay that [3h]ro 15-1788 binds to carp brain with a high degree of anatomical selectivity. saturation binding of the radioligand was determined in seven anatomically defined regions and suggested the presence of one class of binding sites (type i-lke). in general, there was a good correlation between the autoradiographic and the binding data. by far, the optic tectum and the vagal, facial, and glossopharyngeal lobe ... | 2006 | 16298509 |
| the in vitro effects of some pesticides on carbonic anhydrase activity of oncorhynchus mykiss and cyprinus carpio carpio fish. | systemic carbonic anhydrase (ca) inhibitors are among the most powerful agents to lower intraocular pressure. unfortunately, their use is frequently accompanied by undesired side effects. some are due to the relatively large amounts of drug that have to be systematically administered to inhibit the ca in the ciliary processes. the aim of the present work was to study in vitro effects of some pesticides on ca enzyme obtained from blood of fish, which play a key role in salt- and osmoregulation an ... | 2006 | 16298474 |
| preservative effect of combined treatment with electrolyzed nacl solutions and essential oil compounds on carp fillets during convectional air-drying. | the antimicrobial and antioxidant effects on carp fillet samples of treatments with alkaline electrolyzed nacl solution ew (-) prior to treatment with acidic electrolyzed nacl solution ew (+) and 1% solutions of the essential oils consisting of 0.5% carvacrol and 0.5% thymol (1% cv+ty) were tested. first carp fillet samples were treated with ew (-), then ew (+), followed by 1% (c+t), represented as [ew (-)/ew (+)/1% (cv+ty)] for 15 min, during drying at 45 degrees c. samples were subsequently ev ... | 2006 | 16271412 |
| induction of cyp1a mrna in carp (cyprinus carpio) from the kalamazoo river polychlorinated biphenyl-contaminated superfund site and in a laboratory study. | the kalamazoo river superfund site in michigan is contaminated with polychlorinated biphenyls (pcbs), which were heavily discharged into the river from several paper companies as part of the deinking process in the 1950s through 1970s. we characterized biomarkers of chronic pcb exposure in a resident fish population using real-time reverse transcriptase-polymerase chain reaction to examine mrna expression levels of multiple genes in carp (cyprinus carpio) liver from pcb contaminated and referenc ... | 2006 | 16328624 |
| an in vitro study of long-term potentiation in the carp (cyprinus carpio l.) olfactory bulb. | long-term potentiation (ltp) of synaptic transmission is considered a cellular mechanism for neural plasticity and memory formation. previously, we showed that in the carp olfactory bulb, ltp occurs at the dendrodendritic mitral-to-granule cell synapse following tetanic electrical stimulation applied to the olfactory tract, and suggested that it is involved in the process of olfactory memory formation. as a first step towards understanding mechanisms underlying plasticity at this synapse, we exa ... | 2006 | 16328534 |
| the ontogeny of mucosal immune cells in common carp (cyprinus carpio l.). | the ontogeny of carp (cyprinus carpio l.) immune cells was studied in mucosal organs (intestine, gills and skin) using the monoclonal antibodies wcl38 (intraepithelial lymphocytes), wcl15 (monocytes/macrophages) and wci12 (b cells). in addition, recombination activating gene 1 expression was examined in the intestine with real time quantitative pcr and in situ hybridization to investigate extrathymic generation of lymphocytes. wcl38(+) intraepithelial lymphocytes (putative t cells) appeared in t ... | 2006 | 16328358 |
| hif-1alpha and inos levels in crucian carp gills during hypoxia-induced transformation. | hypoxia inducible factor 1 alpha (hif-1alpha) initiates expression of a wide variety of genes, some of which are involved in apoptosis and cell cycle arrest. we have previously shown that crucian carp increases its respiratory surface area 7.5-fold in response to hypoxia. this change is due to apoptosis and cell cycle arrest in specific parts of its gills. here we have characterized crucian carp hif-1alpha, and measured mrna, protein and dna binding levels during hypoxia exposure in crucian carp ... | 2006 | 16362306 |
| molecular cloning and mrna expression analysis of carp embryonic, slow and cardiac myosin heavy chain isoforms. | three embryonic class ii myosin heavy chains (myhs) were cloned from the common carp (cyprinus carpio l.), myhemb1, myhemb2 and myhemb3. myh dna clones were also isolated from the slow muscle of adult carp acclimated to 10 degrees c (myhs10) and 30 degrees c (myhs30). phylogenetic analysis demonstrated that myhemb1 and myhemb2 belonged to the fast skeletal muscle myh clade. by contrast, the sequence of myhemb3 was similar to the adult slow muscle isoforms, myhs10 and myhs30. myhemb1 and myhemb2 ... | 2006 | 16354789 |
| different sensitivity of carp (cyprinus carpio) and rainbow trout (oncorhynchus mykiss) to the immunomodulatory effects of uvb irradiation. | in order to study the sensitivity of two fish species, carp (cyprinus carpio) and rainbow trout (oncorhynchus mykiss), to the immunomodulatory effects of ultraviolet b (uvb) radiation, the fish were exposed to a single uvb dose of 50, 250, 500 or 1,000 mj cm(-2). these species represent different phylogenetic groups of fish, and they differ also in their behaviour inhabitating often dark and turbid (carp) or clear and transparent waters (salmonids). immune responses were studied on day 1 post-ir ... | 2006 | 16376572 |
| toxicity, accumulation, and retention of zinc by carp under normoxic and hypoxic conditions. | zinc is an essential micronutrient that becomes toxic at elevated concentrations. under hypoxic events, i.e., temporal depletion of oxygen, the toxicity of zn increases for fish, apart from the direct effects of the reduced oxygen levels. this enhanced toxicity currently is explained by the increased ventilation rates under hypoxia, causing a higher water flow over the gills. however, the few experimental studies available on the uptake of heavy metals under hypoxia draw contradictory conclusion ... | 2006 | 16494228 |
| down regulation of bisphenol a glucuronidation in carp during the winter pre-breeding season. | environmental pollution by bisphenol a is prevalent in many rivers. the influence of bisphenol a on the reproductive organs of carp has been demonstrated to be serious, especially in the winter pre-breeding season. although bisphenol a is detoxified as bisphenol a-glucuronide in carp organs, principally the intestine, the seasonal variation in the efficiency of the detoxification is not known. to estimate the seasonal risk of bisphenol a in carp, we investigated seasonal changes in microsomal ud ... | 2006 | 16481053 |
| functional analysis on the 5'-flanking region of carp fast skeletal myosin heavy chain genes for their expression at different temperatures. | two types of the fast skeletal myosin heavy chain (myh) genes were cloned from a genomic dna library of carp (cyprinus carpio l.) and named myh10 and myh30, which showed the sequence similarity to the myh cdnas predominantly expressed in carp acclimated to 10 and 30 degrees c, respectively. the 5'-flanking region of about 3 kbp in size each from myh10 and myh30 contained various cis-elements to bind to transcriptional regulatory factors such as myod family and myocyte enhancer factor 2 (mef2) fa ... | 2006 | 16472943 |
| improved uptake of plant-derived ltb-linked proteins in carp gut and induction of specific humoral immune responses upon infeed delivery. | oral vaccination of fish is an effortless and stress free immunisation method which can be used for almost any age. however, vaccination via the mucosal route does have disadvantages. for example, the vaccine may induce tolerance and has to be protected to escape digestion. also the vaccine should be efficiently delivered to immune-competent cells in the gut or other lymphoid organs. in addition, it should be cost effective. here we present a novel fish vaccination model using potato tubers as v ... | 2006 | 16464614 |
| beta-2-microglobulin gene expression is maintained in rainbow trout and atlantic salmon kept at low temperatures. | finfish in the wild are regularly subjected to low temperatures, which have been shown to cause a loss of major histocompatibility receptor expression in common carp kept at 6 degrees c. this is similar to what was seen in a mammalian cell line cultured at 26 degrees c. loss of expression of this critical viral recognition protein may provide one mechanism for the increased frequency of fish diseases at low temperatures. this report demonstrates that unlike carp and mammals, beta(2)m transcript ... | 2006 | 16464613 |
| the presence of multiple and differentially regulated interleukin-12p40 genes in bony fishes signifies an expansion of the vertebrate heterodimeric cytokine family. | interleukin-12 (il-12) is the founding member of a rapidly growing family of heterodimeric cytokines. it consists of two subunits, designated p35 and p40 that together constitute a disulphide-linked heterodimeric cytokine. il-12 is well known for its prominent role in both the early innate immune response and the skewing of the ensuing acquired immune response towards th1. here, we report the presence of il-12p35 and three highly distinct il-12p40 genes in common carp (cyprinus carpio). the carp ... | 2006 | 16460805 |
| sublethal effects of organophosphate diazinon on the brain of cyprinus carpio. | effects of diazinon, at different concentrations and exposure times, were investigated in freshwater fish, cyprinus carpio, to elucidate the possible mode of action on lipid peroxidation together with the inhibitory effect of diazinon on acetylcholinesterase activity and changes in tissue protein levels. cholinesterase inhibition is considered to be a specific biomarker of exposure to organophosphorus pesticides. fish were exposed to 0.0036 microg/l, 0.018 microg/l, and 0.036 microg/l (sublethal ... | 2006 | 16455590 |
| assessment of effects in mink caused by consumption of carp collected from the saginaw river, michigan, usa. | polychlorinated hydrocarbons, including polychlorinated biphenyls (pcbs), polychlorinated dibenzo-p-dioxins (pcdds), and polychlorinated dibenzofurans (pcdfs), are ubiquitous environmental contaminants that bioconcentrate in the food chain. numerous studies have demonstrated mink (mustela vison) to be one of the most sensitive species to this group of compounds. in recent studies, a lesion characterized by osteoinvasion of epithelial cells into the mandible and maxilla of young mink fed diets co ... | 2006 | 16418898 |
| exposure of spermatozoa to duroquinone may impair reproduction of the common carp (cyprinus carpio) through oxidative stress. | toxicity of many waterborne organic contaminants to aquatic organisms is mediated through oxidative damages resulting from the production of reactive oxygen species (ros). using duroquinone as a model ros inducer, we carried out in vitro and in vivo experiments to test the hypothesis that reproduction in common carp (cyprinus carpio) can be impaired through oxidative damage of their spermatozoa. in vitro exposure of fish spermatozoa to 0, 12.5, 25, 50, 100 and 200 microm duroquinone for 2 h show ... | 2006 | 16413940 |
| ontogeny of the common carp (cyprinus carpio l.) innate immune system. | the ontogeny of the teleost innate immune system was studied in carp using cellular, histological and quantitative molecular techniques. carp myeloid cells first appeared ventro-lateral of the aorta at 2 days post fertilization (the start of hatching), and subsequently around the sinuses of the vena cardinalis (or posterior blood islet), head kidney and trunk kidney. in addition, the hematopoietic tissue around the sinuses of the vena cardinalis transformed into that of the trunk kidney, which i ... | 2006 | 16406121 |
| crf and stress in fish. | the endocrine stress response is pivotal in vertebrate physiology. the stress hormone cortisol-the end product of the endocrine stress axis-(re-)directs energy flows for optimal performance under conditions where homeostasis may be or become at risk. key players in the continuous adaptation process are corticotropin-releasing factor (crf) from the hypothalamic nucleus preopticus (npo), pituitary adrenocorticotropic hormone (acth) and cortisol produced by the interrenal cells in the headkidney (a ... | 2006 | 16403502 |
| in vitro study of amp-deaminase from fish (cyprinus carpio) treated with hydrolyzable tannins. | amp-deaminase (ec 3.5.4.6) is an enzyme responsible for stabilising adenylate energy changes. the properties of this enzyme are controlled by various ligands of hydrophobic nature. an investigation of enzyme activity alterations under the influence of natural phenolic acids (tannic, ellagic and gallic) which are soluble in water, could evidence the biological toxicity of these compounds. in our study purified amp-deaminase isolated from white muscle of cyprinus carpio was exposed to phenolic aci ... | 2006 | 16646524 |
| development and validation of a direct homologous quantitative sandwich elisa for fathead minnow (pimephales promelas) vitellogenin. | vitellogenin (vtg) is an established and sensitive endpoint for analysis of exposure to (anti-)oestrogens and their mimics in fish [sumpter, j.p., 1995. feminized responses in fish to environmental estrogens. toxicol. lett. 82, 737-742; arukwe, a., goksøyr, a., 2003. eggshell and egg yolk proteins in fish: hepatic proteins for the next generation: oogenetic, population, and evolutionary implications of endocrine disruption. comp. hepatol. 2, 4. ]. in some instances, links have been drawn between ... | 2006 | 16644026 |
| valsartan cardio-renal protection in patients undergoing coronary angiography complicated with chronic renal insufficiency (val-carp) trial: rationale and design. | despite an increase in the frequency of coronary angiography (cag) in japan, the exact incidence of contrast-induced nephropathy (cin) remains unknown in the japanese population, especially in patients with chronic renal insufficiency. in addition, the nature of pharmacological interventions that would benefit the patients before or after procedures such as coronary bypass graft (cabg) and percutaneous coronary intervention (pci) has not been fully investigated. | 2006 | 16636488 |
| determination of the big head carp myofibrillar (aristichthys nobilis) adenosine triphosphatase activity by ion chromatography. | a method for the determination of adenosine triphosphatase (atpase) activity of myofibrils of big head carp by using ion chromatography was introduced. adenosine triphosphate (atp), adenosine diphosphate (adp) and orthophosphate (pi) were separated completely. recoveries for atp, adp and pi were 98+/-5%, 97+/-4% and 98+/-5%, respectively. pi liberated from atp during reaction was monitored by ion chromatography using the suggested method. this method was applicable to the determination of myofib ... | 2006 | 16701679 |
| three-dimensional visualization of a mandibular first molar with three distal roots using computer-aided rapid prototyping. | nonsurgical endodontic therapy of a right mandibular first molar with 3 distal roots was successfully performed with the aid of magnification. 3d data (dicom format) of the tooth were obtained from a ct highspeed advantage and a denta scan program produced by ge medical systems. the ct protocol used for this procedure involved a slit thickness of 1 mm. the 3d digital data obtained were fed into a visualization program (v-works; cybermed co) and then exported to the rapid prototyping machine for ... | 2006 | 16632282 |
| cadmium and zinc induction of znt-1 mrna in an established carp cell line. | the cdna of a zinc transporter-1 (znt-1) gene was cloned from an established cell line derived from common carp (cyprinus carpio) using rapid amplification of cdna ends (race). using real-time quantitative pcr, we showed that both zinc (zn) and cadmium (cd) transiently upregulate znt-1 mrna to comparable levels. the loosely bound cellular zn pool, as estimated using the zn-specific probe fluozin-3, was increased threefold after exposure to 250 microm zncl(2). correspondingly, the znt-1 mrna leve ... | 2006 | 16627006 |
| [toxic hepatitis associated with carp juice ingestion]. | the potential hepatotoxicity of herbal remedies and/or health foods is usually ignored in daily life. there have been cases showing the toxic hepatitis and renal failure associated with the ingestion of raw carp bile. we experienced a case of toxic hepatitis without any evidence of renal failure that was associated with carp juice ingestion. the clinical manifestations were characterized by nausea and vomiting after the ingestion of carp juice for 3 months. the diagnosis of toxic hepatitis was m ... | 2006 | 16565611 |
| ultrastructural changes of the carp (cyprinus carpio) hepatocyte nucleolus during seasonal acclimatization. | the eurythermal fish carp (cyprinus carpio) adjusts to the seasonal changes in the temperature and photoperiod of its habitat through diverse cellular and molecular mechanisms. we have observed that ribosomal biogenesis is modulated during the acclimatization process and correlates with profound phenotypic changes, reflecting a seasonal-dependent ultrastructural appearance of the nucleolar components. previous studies using classical techniques showed that in winter-adapted carp the nucleolus ap ... | 2006 | 16563129 |
| evidence for melano-macrophage centres of teleost as evolutionary precursors of germinal centres of higher vertebrates: an immunohistochemical study. | the melano-macrophage centres (mmcs) of the haemolymphopoietic organs of teleost fish trap and retain antigens and are closely associated with immunoglobulin-secreting cells. the hypothesis that they are the phylogenetic precursors of the germinal centres of higher vertebrates has been questioned due to their apparent lack of organising cells. in this study the immunoreactivity of mmc cells from spleen and kidney of the teleosts cyprinus carpio, odontesthes bonariensis and solea senegalensis to ... | 2006 | 16533606 |