Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| the intestine and rheumatism. | arthritis following certain salmonella infections, dysentery, and nonspecific enterocolitis exemplifies the fact that intestinal infections can elicit rheumatic reactions. recent observations indicate that in the last mentioned group specific microbes, such as clostridium perfringens and yersinia enterocolitica, may be involved. references are made to the findings of an abnormal intestinal flora, notably c. perfringens, in patients with chronic arthritis and of circulating and cell-bound antibod ... | 1987 | 20144107 |
| effects of exogenous phospholipases on brain membrane phospholipid perturbation, (na(+) + k(+))-atpase activity and cellular swelling of brain slices. | rat brain membranes were incubated with bee venom phospholipase a(2) (pla(2)) or phospholipase c (plc) from clostridium perfringens. pla(2) caused a significant increase in free polyunsaturated fatty acids concomitant with membrane phospholipid degradation as monitored by hplc and by gas chromatography. equal concentrations of plc had a much lesser effect than pla(2). divergent and differential effects were shown on deacylation and incorporation of [(3)h]arachidonic acid in membrane phospholipid ... | 1987 | 20501100 |
| [fatal septicemia caused by intra-uterine device in aplasia of acute leukemia]. | 1988 | 2905060 | |
| [inhibition of cytochrome p-450 and cytochrome b5 induced by clostridium perfringens alpha toxin]. | 1988 | 2905072 | |
| isolation of fecal clostridium perfringens from broiler chickens and their susceptibility to eight antimicrobial agents for growth promotion. | 1988 | 2905396 | |
| in vitro lecithinase activity and sensitivity to 22 antimicrobial agents of clostridium perfringens isolated from necrotic enteritis of broiler chickens. | viable clostridium perfringens ranging from 10(5) to 10(8) g-1 was detected in all of 88 intestinal content specimens of necrotic enteritis in broiler chickens. in vitro lecithinase activity and sensitivity to 22 antimicrobial agents were determined for the 88 isolates. the activities of lecithinase in the culture filtrate of isolates were estimated to be 0.5 to 4.0 ae ml-1 as alpha-antitoxin equivalent. with reference to antimicrobial activity penicillins and cephazolin showed excellent activit ... | 1988 | 2905509 |
| food poisoning in hospitals in scotland, 1978-87. | during the 10-year period 1978-87 there were 48 outbreaks of food poisoning in scottish hospitals affecting a total of 2287 persons of whom 12 died. this compared with 50 outbreaks during the previous 5 years (1973-77) when over 1500 persons and 7 deaths were recorded. although the incidence of outbreaks has decreased the average number of persons affected in outbreaks has increased. a marked reduction was seen in the incidence of outbreaks due to clostridium perfringens, in contrast to foodborn ... | 1988 | 2905670 |
| [an autopsy case of clostridial liver abscess]. | 1988 | 2905727 | |
| purification, characterization and antimicrobial spectrum of a bacteriocin produced by pediococcus acidilactici. | an antimicrobial peptide designated pediocin ach was isolated from pediococcus acidilactici strain h. the pediocin ach was purified by ion exchange chromatography. the molecular weight of pediocin ach was determined by sds-page to be about 2700 daltons. pediocin ach was sensitive to proteolytic enzymes resistant to heat and organic solvents, and active over a wide range of ph. pediocin ach exhibited inhibition against several food spoilage bacteria and foodborne pathogens including staphylococcu ... | 1988 | 2906056 |
| the effect of carbohydrates on the sporogenesis of clostridium perfringens and bacillus anthracis. | the authors studied the effect of a number of carbohydrates on the sporogenesis of clostridium perfringens and bacillus anthracis (vaccine strain sti) as probable soil factors capable of influencing the duration of survival of these causative agents in the external environment. differences in the effect of the same sugars on the formation of spores by these microorganisms and clearly expressed sporogenesis-inhibiting effect of glucose (and also of lactose in clostridia) have been demonstrated. t ... | 1988 | 2906076 |
| assay methods for alpha toxin from clostridium perfringens: phospholipase c. | 1988 | 2906729 | |
| purification of alpha toxin from clostridium perfringens: phospholipase c. | 1988 | 2906730 | |
| production, purification, and assay of clostridium perfringens enterotoxin. | 1988 | 2906731 | |
| microbiological and virological analysis of water from two water filtration plants and their distribution systems. | the microbial flora of the water produced by two water filtration plants and their drinking water distribution system were evaluated: the pont-viau (pv) and the repentigny (re) water filtration plants. untreated water entering the plants contained 3.6 (pv) and 16.8 most probable number of infectious units (mpniu)/l (re) enteric viruses and total coliform bacteria counts were 300,000 (pv) and 500,000 cfu/l (re). treated water leaving the plant was essentially free of all the bacterial indicators ... | 1988 | 2906813 |
| clostridial gas gangrene: a review of 48 consecutive cases. | 1988 | 2907224 | |
| clostridium perfringens enterotoxin. | current knowledge of cpe action is briefly summarized in figure 1. after specific binding to a protein receptor(s), the entire cpe molecule rapidly inserts into membranes forming a complex of 150,000 mr. almost simultaneously with insertion, there is a sudden change in ion fluxes. the molecular events behind the induction of ion flux changes remain undefined, but might involve either direct formation of membrane pores by cpe or activation of pre-existing membrane pores. as intracellular ion leve ... | 1988 | 2907364 |
| chemotherapy of experimental (murine) clostridium perfringens type a gas gangrene. | five antimicrobial drugs (ciprofloxacin, clindamycin, imipenem, penicillin g, and rifampin) were examined for therapeutic efficacy in a murine model gas gangrene due to clostridium perfringens type a, following infection with large bacterial inocula. imipenem was effective against all 6 strains of c. perfringens; conversely, penicillin g failed against these 6 strains. ciprofloxacin, clindamycin, and rifampin occupied intermediate positions. | 1988 | 2907452 |
| [growth of clostridium perfringens in the guinea pig intestinal juice medium]. | 1988 | 2907757 | |
| [use of the elisa test for detection of toxins of clostridium perfringens type a]. | 1988 | 2907767 | |
| [in vitro activity of roxithromycin against hospital bacteria and the concordance curve]. | this study was set up to establish the regression curve for roxithromycin inhibition zone diameters (disks 15 micrograms) and mic to create a strain distribution plot, in order to allow accurate interpretation of the disk diffusion method for testing susceptibility to roxithromycin. 373 bacterial strains were studied in three university hospital. roxithromycin was active against erythromycin sensitive staphylococcus aureus and coagulase negative staphylococci at concentrations of 0.06 to 4 micro ... | 1988 | 2900488 |
| [clostridium perfringens septicemia]. | we report 3 cases of clostridium perfringens bacteremia with uterine gas gangrene. clinical presentation included severe infectious syndrome, hemoglobinemia and hemoglobinuria, jaundice, uterine tenderness and hypertension. all 3 cases were first seen with installed renal failure. diagnosis and modalities of therapy were reviewed. clostridium perfringens bacteremia with uterine gas gangrene still occur in developing countries. | 1988 | 2900547 |
| on the in vitro neutralization test of clostridium perfringens type a toxin. | data are presented on the detection in crude animal and human sera of cl. perfringens phospholipase c (plc) inhibitor. when the level of cl. perfringens type a antitoxin is determined in the in vitro toxin neutralization test the inhibitor is found to decrease plc activity in the test dose of experimental homologous toxin. the extent of decrease accounts for the variation of results obtained in the in vitro and in vivo toxin neutralization tests. the variation may be cancelled out by introducing ... | 1988 | 2900852 |
| lyophilized clostridium perfringens 3 alpha- and clostridium bifermentans 7 alpha-hydroxysteroid dehydrogenases: two new stable enzyme preparations for routine bile acid analysis. | preparations of 3 alpha-hydroxysteroid dehydrogenase (ec 1.1.1.50) from clostridium perfringens were successfully lyophilized into a stable powder form. purification of the enzyme was achieved using triazine dye affinity chromatography. c. perfringens 3 alpha-hydroxysteroid dehydrogenase was purified 24-fold using reactive red 120 (procion red) -cross-linked agarose (70% yield). quantitative measurement of bile acids with the purified enzymes, 3 alpha-hydroxysteroid dehydrogenase and 7 alpha-hyd ... | 1988 | 2901274 |
| protease-nicked theta-toxin of clostridium perfringens, a new membrane probe with no cytolytic effect, reveals two classes of cholesterol as toxin-binding sites on sheep erythrocytes. | a nicked theta-toxin (c theta), obtained by limited proteolysis with subtilisin carlsberg, causes almost no hemolysis while it retains a nearly intact cholesterol binding site below 20 degrees c. neither electron microscopic evidence for the formation of arc- and ring-shaped structures on the membrane nor toxin-stimulated influx of extracellular ca2+ are detected in c theta-treated cells below 20 degrees c. thus, event(s) in the lytic process are responsible for the temperature dependency of hem ... | 1988 | 2901352 |
| adp-ribosylated actin caps the barbed ends of actin filaments. | the mode of action on actin polymerization of skeletal muscle actin adp-ribosylated on arginine 177 by perfringens iota toxin was investigated. adp-ribosylated actin decreased the rate of nucleated actin polymerization at substoichiometric ratios of adp-ribosylated actin to monomeric actin. adp-ribosylated actin did not tend to copolymerize with actin. actin filaments were depolymerized by the addition of adp-ribosylated actin. the maximal monomer concentration reached by addition of adp-ribosyl ... | 1988 | 2901417 |
| hybridization analysis of the class p tetracycline resistance determinant from the clostridium perfringens r-plasmid, pcw3. | the tetracycline resistance determinant from pcw3, a conjugative plasmid from clostridium perfringens, has been identified and the structural gene localized to within a 1.4-kb region. hybridization analysis, which utilized an internal 0.8-kb specific gene probe, showed that eight nonconjugative tetracycline resistant c. perfringens strains all carried homologous resistance determinants. no homology was detected in dna prepared from tetracycline resistant isolates of clostridium difficile or clos ... | 1988 | 2901767 |
| complete nucleotide sequence and genetic organization of the bacteriocinogenic plasmid, pip404, from clostridium perfringens. | the complete nucleotide sequence of the bacteriocinogenic plasmid, pip404, from clostridium perfringens has been determined. the plasmid genome comprises 10,207 bp and has a da + dt content of 75%. functions have been tentatively assigned to 6 of the 10 open reading frames and an origin-like region of repeated sequence identified. the codon usage of this extremely da + dt rich plasmid is highly unusual and displays a pronounced preference for codons with the lowest dg + dc content. only one of t ... | 1988 | 2901768 |
| identification and molecular genetic analysis of replication functions of the bacteriocinogenic plasmid pip404 from clostridium perfringens. | the replication functions of the bacteriocinogenic plasmid pip404, from clostridium perfringens, were localized to a 2.8-kb ecori-ecorv fragment by cloning into a vector deficient for replication in bacillus subtilis. this fragment contains two genes, cop and rep, which encode proteins and an 800-bp noncoding segment of complex structure consisting of multiple tandemly repeated sequences. the cop protein is involved in copy number control, whereas the rep gene product is essential for plasmid re ... | 1988 | 2901769 |
| the clostridium perfringens chloramphenicol resistance transposon tn4451 excises precisely in escherichia coli. | nucleotide sequence analysis of the tn4451-deletion derivatives, pjir47 and pjir86, which were derived from escherichia coli and clostridium perfringens, respectively, showed that the deletion events that led to the formation of these plasmids were identical and precise. the results also showed that the termini of this c. perfringens-derived transposon contained imperfect 12-bp inverted repeat sequences which had some sequence similarity with the termini of tn3-like transposons. | 1988 | 2901770 |
| cloning and sequencing of a clostridium perfringens sialidase gene. | escherichia coli was transformed with puc vectors containing sau3a restriction fragments (rf) of clostridium perfringens dna. two clones expressed sialidase activity when assayed with the fluorogenic substrate 4-methylumbelliferyl-alpha-d-n-acetylneuraminic acid. a synthetic oligonucleotide representing the n-terminus of the expressed enzyme hybridized with the clostridial insert and with a corresponding 2.1 kb sau3a rf of the c. perfringens genome. the insert reduced to 1.4 kb, which still enco ... | 1988 | 2901987 |
| rubredoxin as an intermediary electron carrier for nitrate reduction by nad(p)h in clostridium perfringens. | the nad(p)h-dependent nitrate reductase system in clostridium perfringens was reconstituted with rubredoxin (rd), nitrate reductase (nar), and an unadsorbed fraction, on a deae-cellulose column, of the extract (designated as fraction a), under nitrogen gas. ferredoxin in place of rd was not effective as an electron carrier in this reconstituted system. nad(p)h-dependent nitrate reducing activity was also obtained by replacing fraction a with ferredoxin-nadp+ reductase from spinach. we propose th ... | 1988 | 2902073 |
| the production and evaluation of monoclonal antibodies to clostridium perfringens type d epsilon toxin. | the production of five monoclonal antibodies to the epsilon prototoxin of clostridium perfringens is described. all five monoclonal antibodies located three proteins in a trypsinized preparation of epsilon prototoxin. these proteins were located at 37.6 kdal, 35.6 kdal and 33.7 kdal by western blots. two of the monoclonal antibodies, m26/2 and m27/12, neutralized epsilon toxin in the mouse lethality assay. four of the five monoclonal antibodies are considered suitable as reagents to detect epsil ... | 1988 | 2902095 |
| [clostridium perfringens sepsis and hypernephroma]. | 1988 | 2902262 | |
| [isolation and purification of phospholipase c from clostridium perfringens and antibodies against it using polymeric biospecific adsorbents]. | a new polymeric biospecific adsorbent intended for isolation of clostridium perfringens phospholipase c (plc) and plc-specific antibodies is discussed. it was obtained by radical copolymerization of acrylamide, methylenbisacrylamide, and the acryl acid chloranhydride-acylated substrate of plc (chicken yolk lecithovitellin). maximal adsorption of plc was observed in the presence of the enzyme activator, and the highest amount of plc was eluted in case of its minimal adsorption. the "residual" act ... | 1988 | 2902618 |
| activation of clostridium perfringens spores under conditions that disrupt hydrophobic interactions of biological macromolecules. | the effect of hydrophobic interactions on the activation of c. perfringens nctc 8679 spores was examined by heating spores under conditions that modify the hydrophobic properties of biological macromolecules. after the activation treatment and a washing procedure, germination was determined by measuring the decrease in optical density of spores suspended in an enriched germination medium. activation was inhibited for spores that were treated under conditions that strengthen hydrophobic interacti ... | 1988 | 2902828 |
| the in-vitro activity of three long-acting cephalosporins against bacteroides fragilis, peptostreptococcus species and clostridium perfringens. | the in-vitro activity of three long-acting cephalosporins (cefotetan, cefonicid and ceftriaxone) was compared against 206 clinical isolates of bacteroides fragilis, peptostreptococcus species and clostridium perfringens, using an agar dilution procedure to determine minimum inhibitory concentrations (mics). clindamycin was included as a comparator. cefotetan was much more active than the two other cephalosporins against strains of bacteroides fragilis (mic90 16 mg/l compared with greater than 12 ... | 1988 | 2902937 |
| cloning and expression in escherichia coli of the perfringolysin o (theta-toxin) gene from clostridium perfringens and characterization of the gene product. | the gene encoding perfringolysin o, the thiol-activated hemolysin from clostridium perfringens (atcc 13124), was cloned and expressed in escherichia coli. a gene library of c. perfringens chromosomal dna was constructed in bacteriophage lambda embl3. a recombinant was identified that produced a hemolysin that was inhibited by cholesterol and was tentatively identified as perfringolysin o. subcloning experiments localized the perfringolysin o gene (pfo) to a 1.8-kilobase region on the cloned chro ... | 1988 | 2903127 |
| nucleotide sequence of the gene for perfringolysin o (theta-toxin) from clostridium perfringens: significant homology with the genes for streptolysin o and pneumolysin. | the nucleotide sequence was determined for the gene encoding the thiol-activated cytolysin, perfringolysin o (theta-toxin), from clostridium perfringens. the nucleotide-sequence-derived primary structure of perfringolysin o is 499 residues long and exhibits a 27-amino-acid signal peptide. the calculated molecular weight of the secreted (mature) form of perfringolysin o is 52,469. the deduced amino-terminal sequence of perfringolysin o is identical to that determined for purified perfringolysin o ... | 1988 | 2903128 |
| inhibitory effect of taurolipids on clostridium perfringens sialidase. | taurolipids a and b, which are detergent-type compounds isolated from protozoan tetrahymena cells, were demonstrated to inhibit strongly the activity of clostridium perfringens sialidase. on addition of 280 pmol of taurolipid b to 20 mu of the enzyme, the sialidase activity was decreased to 7% of the original activity at ph 5.1 as the optimum ph. the inhibition was non-competitive. effective inhibition was observed at the acidic region from the isoelectric point of the sialidase, and at a low io ... | 1988 | 2903144 |
| purification and characterization of bile salt hydrolase from clostridium perfringens. | bile salt hydrolase (cholylglycine hydrolase, ec 3.5.1.24) has been purified to homogeneity (792-fold) from clostridium perfringens using high performance deae-chromatography. the purified enzyme showed a single detectable protein band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) with a relative molecular weight ca. 56,000. the intact enzyme had a relative molecular weight (mr) of ca. 250,000 as determined by nondenaturing page. the nh2-terminal sequence of bile salt h ... | 1988 | 2903208 |
| effect of product form on the microbiological growth support characteristics of turkey meat products. | effects of product form classifications, consisting of intact turkey drumsticks, dark meat trim tissue, and mechanically deboned meat, on microbiological concentrations were evaluated. samples were inoculated with actively growing cultures of salmonella and clostridium perfringens to increase naturally low bacterial populations prior to being placed at 1 c and 10 c for storage evaluations. growth support potential for all product forms stored at 10 c was extensive, making it difficult to charact ... | 1988 | 2903493 |
| [a case of food poisoning caused by c. perfringens]. | a case of food borne infection among a hundred inhabitants of a home for the old aged, caused by clostridium perfringens (clostridium welchii) following consumption of a filled veal roll is reported. | 1988 | 2903578 |
| electroporation-induced transformation of intact cells of clostridium perfringens. | electroporation-induced transformation of intact cells of clostridium perfringens 3624a with plasmids pamb1 and phr106 resulted in 3.8 x 10(-5) and 4.2 x 10(-4) transformants per viable cell, respectively. with respect to shuttle plasmid phr106, these values represent a greater than 100-fold increase in transformation frequency when compared with the values reported with polyethylene glycol-induced l-phase variants. | 1988 | 2903718 |
| clostridium absonum from gas gangrene. | a clostridium perfringens-like strain was isolated from a case of gas gangrene. the morphological properties and the lecithinase reaction of the isolate were very similar to those of c. perfringens; however, the lecithinase reaction was only slightly suppressed by c. perfringens alpha-antitoxin serum and the organism was identified as clostridium absonum from its biochemical properties. | 1988 | 2903940 |
| effect of eimeria tenella infection on the caecal population of lincomycin-resistant clostridium perfringens introduced into chickens. | the effect of eimeria tenella infection on the caecal population of lincomycin-resistant clostridium perfringens (kgw 851) newly introduced into chickens was studied. four groups of chickens consisted of: (1) uninoculated controls; (2) inoculated with c perfringens (kgw 851); (3) inoculated with e tenella; and (4) inoculated with c perfringens (kgw 851) followed by e tenella. five chickens in each group were necropsied on each of days 0, 3, 5, 7, 10 and 14 after the e tenella inoculation. the me ... | 1988 | 2904158 |
| enterotoxaemia in a foal due to clostridium perfringens type a. | 1988 | 2904258 | |
| divalent cation involvement in the action of clostridium perfringens type a enterotoxin. early events in enterotoxin action are divalent cation-independent. | clostridium perfringens type a enterotoxin (cpe) is a membrane-active cytotoxin. there are a number of recognized early steps in cpe cytotoxicity including binding of cpe to a protein receptor, insertion of cpe into membranes, and cpe-mediated induction of changes in membrane permeability for small molecules such as ions and amino acids. further support for the existence of these early steps and further characterization of these events are presented in this report. we now report that these early ... | 1988 | 3123494 |
| attachment of human placental-type alkaline phosphatase via phosphatidylinositol to syncytiotrophoblast and tumour cell plasma membranes. | phosphatidylinositol anchors human placental-type alkaline phosphatase (plap) to both syncytiotrophoblast and tumour cell plasma membranes. plap activity was released from isolated human placental syncytiotrophoblast plasma membranes and the surface of tumour cells with a phospholipase c from bacillus cereus. this was a specific event, not the result of proteolysis or membrane perturbation, but the action of a phosphatidylinositol-specific phospholipase c in the preparation. soluble plap, releas ... | 1988 | 3127211 |
| contribution of the microflora to proteolysis in the human large intestine. | protease activities in human ileal effluent were approximately 20-fold greater than in normal faeces. comparative studies with faeces from a person who did not have a pancreas suggested that a substantial proportion of the proteolytic activity in normal faeces was of bacterial origin. thimerosal, iodoacetate, edta and cysteine significantly inhibited proteolysis in faeces, but not in small intestinal contents, showing that cysteine and metalloproteases were produced by bacteria in the large gut. ... | 1988 | 3127369 |
| phospholipase activation and arachidonic acid release in isolated intestinal epithelial cells. | a novel method for studying the mobilization of free arachidonic acid (aa) in isolated intestinal epithelial cells is described. the method is based on labeling the cellular phospholipids with 14c-aa and studying the release of this 14c-aa on subsequent phospholipase activation. cells of high viability were isolated from the small intestine of guinea pigs and incubated with 14c-aa for 2 h; most of the incorporated 14c-aa was then esterified into phosphatidylethanolamine and phosphatidylcholine. ... | 1988 | 3132736 |
| actin-specific adp-ribosyltransferase produced by a clostridium difficile strain. | by screening possible adp-ribosyltransferase activities in culture supernatants from various clostridium species, we have found one clostridium difficile strain (cd196) (isolated in our laboratory) that is able to produce, in addition to toxins a and b, a new adp-ribosyltransferase that was shown to covalently modify cell actin as clostridium botulinum c2 or clostridium perfringens e iota toxins do. the molecular weight of the cd196 adp-ribosyltransferase (cdt) was determined to be 43 kilodalton ... | 1988 | 3137166 |
| membrane phospholipid polar heads influence the coupling of m2 muscarinic receptors to g proteins. | treating membranes from rat heart with phospholipase c (phosphatidylcholine choline phosphohydrolase) from clostridium perfringens increased the affinity of muscarinic acetylcholine receptors (m2) for the agonists carbachol and oxotremorine. the affinity for antagonists was not affected. phospholipase c activity, i.e., the cleavage of polar heads of membrane phospholipids, led to the disappearance of the guanine nucleotide-dependent rightward shift of the isotherm for agonist binding. the treatm ... | 1988 | 3140043 |
| construction of shuttle cloning vectors for bacteroides fragilis and use in assaying foreign tetracycline resistance gene expression. | shuttle vectors capable of replication in both escherichia coli and bacteroides fragilis have been developed. conjugal transfer of these plasmids from e. coli to b. fragilis is facilitated by inclusion of the origin of transfer of the incp plasmid rk2. the vectors pdk1 and pdk2 provide unique sites for cloning selectable markers in bacteroides. poa10 is a cosmid vector containing the replication region of pcp1 necessary for maintenance in bacteroides. pdk3, pdk4.1, and pdk4.2 contain the bactero ... | 1988 | 3071818 |
| intracellular processing of cytidylyltransferase in krebs ii cells during stimulation of phosphatidylcholine synthesis. evidence that a plasma membrane modification promotes enzyme translocation specifically to the endoplasmic reticulum. | after a 3-h incubation of krebs ii ascitic cells in the presence of phospholipase c from clostridium welchii under nonlytic conditions, the incorporation of [3h] choline into phosphatidylcholine was increased 1.7-fold as compared to untreated cells. the total amounts of phosphatidylcholine, phosphatidylethanolamine, and sphingomyelin were unchanged up to 3 h of incubation. the limiting step in phosphatidylcholine biosynthesis was the formation of cdp-choline catalyzed by ctp:choline-phosphate cy ... | 1988 | 2893798 |
| nonimmune binding of ig to clostridium perfringens. preferential binding of igm and aggregated igg. | in an attempt to find a bacterial igm receptor, a large number of bacterial strains of different species were screened for the ability to bind human igm. certain strains of the anaerobic bacterium clostridium perfringens were found to bind a major fraction of polyclonal igm. one bacterial strain showed a particularly high binding capacity and was studied in more detail. this strain is also able to bind a minor fraction of polyclonal iga and igg. inhibition experiments indicate that the different ... | 1988 | 2893826 |
| high resolution deuterium nmr studies of bacterial metabolism. | high resolution deuterium nmr spectra were obtained from suspensions of five bacterial strains: escherichia coli, clostridium perfringens, klebsiella pneumoniae, proteus mirabilis, and staphylococcus aureus. deuterium-labeled d-glucose at c-1, c-2, and c-6 was used to monitor dynamically anaerobic metabolism. the flux of glucose through the various bacterial metabolic pathways could be determined by following the disappearance of glucose and the appearance of the major end products in the 2h nmr ... | 1988 | 2904438 |
| clostridium perfringens infection in a total knee arthroplasty. a case report. | a 66-year-old woman with a painful total knee arthroplasty and turbid fluid aspirates had her prosthesis removed for a presumed diagnosis of infection. the intraoperative cultures were positive for clostridium perfringens, and the patient did well after a course of intravenous antibiotics prior to reimplantation. clostridium perfringens is a rare cause of pyarthrosis in both nonoperative and prosthetic joints. this report details the first case of clostridial infection involving a patient with a ... | 1988 | 2904487 |
| gas gangrene in a diabetic after intramuscular injection. | a 47 year old male insulin-dependent diabetic developed two synchronous life-threatening clostridium perfringens infections under unusual circumstances. numerous sterile abscesses were also present, being the result of regular pentazocine intramuscular injections. extensive surgical debridement and parenteral antibiotic therapy proved effective in treating the septicaemia and the large soft tissue abscesses in the buttock and thigh. the patient made an excellent functional recovery. the pathogen ... | 1988 | 2908251 |
| [microbial contamination of traditional herbal drugs in indonesia]. | 1988 | 2907976 | |
| some factors affecting isolation of clostridium tetani from human and animal stools. | clostridium tetani was isolated from human and animal stools at the following rates [95% confidence interval (ci)]: human, 0% (1.5-0); horse, 1% (5-0); cow in cowshed, 4% (10-1); cow in pasture, 8.3% (17-1), calf in pasture, 0% (7-0); dog, 2% (11-0) and sheep in pasture, 25% (44-14). quantification of c. tetani in 16 animal stools positive for the bacillus was impossible in most cases, as the number of tetanus bacilli present was not large enough for this purpose. contaminating anaerobic and fac ... | 1988 | 2908185 |
| [purulent pleurisy caused by anaerobic bacteria. a retrospective study of 19 cases]. | a retrospective clinical, biological, radiological and evolutive study of 19 cases of pleural empyema caused by anaerobic organisms diagnosed between 1980 and 1986 was carried out. these 19 cases accounted for 30.6 p. 100 of all cases of pleural empyema diagnosed during the same period. a local or general contributory factor was found in all patients; false passage, gastrointestinal pathology and buccal or dental diseases were the most frequent aetiological circumstances. the clinical picture wa ... | 1988 | 2967476 |
| microbiological quality of fresh pasta dumplings sold in bologna and the surrounding district. | the microbiological quality of fresh pasta dumplings sold in bologna and the surrounding district was evaluated. a total of 60 lots (300 subsamples) of fresh pasta dumplings, both 'home-made' and manufactured, were analysed for aerobic plate count (apc), coliforms (total and fecal), staphylococcus aureus, clostridium perfringens and salmonella spp. thirty one of the 39 lots of 'home-made' pasta were found to exceed apc standards and six lots exceeded s. aureus standards. five (24%) and six (29%) ... | 1988 | 2908612 |
| studies on metabolic regulation and estimation of sialic acids by enzyme immobilization techniques. | 1988 | 2896358 | |
| human platelet activation by bacterial phospholipase c: mechanism of inhibition by flurazepam. | we have shown earlier that phospholipase c (plc) from clostridium perfringens causes platelet activation possibly by inducing turnover of phosphoinositides and phosphorylation of a 47,000 dalton protein (p47). moreover, only 15 microm and 11 microm flurazepam inhibits plc-induced platelet aggregation and serotonin secretion by 50% respectively. this study was conducted to better understand the mechanism of platelet activation by plc and its inhibition by flurazepam. incubation of (14c)-arachidon ... | 1988 | 2896397 |
| inhibition of n-acetylneuraminate lyase by n-acetyl-4-oxo-d-neuraminic acid. | we show that the 4-oxo analogue of n-acetyl-d-neuraminic acid strongly inhibits n-acetylneuraminate lyase (neuac aldolase, ec 4.1.3.3) from clostridum perfringens (ki = 0.025 mm) and escherichia coli (ki = 0.15 mm). in each case the inhibition was competitive. n-acetyl-d-neuraminic acid; n-acetylneuraminate lyase; n-acetyl-d-neuraminic acid analog; 5-acetamido-3,5-dideoxy-beta-d-manno-non-2,4-diulosonic acid; 2-deoxy-2,3-didehydro-n-acetyl-4-oxo-neuraminic acid; competitive inhibitor. | 1988 | 2896604 |
| evaluation of in vitro methods for testing ceftriaxone against anaerobic bacteria, including quality control guidelines. | tests with 100 anaerobic bacterial isolates demonstrated comparability between ceftriaxone mics obtained with the reference agar dilution procedure and those obtained with a broth microdilution susceptibility testing procedure. the aerobically incubated thioglycolate disk elution test was also evaluated. six 30-micrograms ceftriaxone disks in 5 ml of thioglycolate separated strains for which mics were less than or equal to 32 micrograms/ml from those for which mics were greater than or equal to ... | 1988 | 2896669 |
| influence of clostridium perfringens and its toxin in germ-free chickens. | twenty-one of 56 germ-free chickens died after receiving an oral inoculation of a broth culture of clostridium perfringens. at necropsy there was detachment and disruption of the epithelial layer at the tips of villi and sloughed epithelial cells in the duodenum. these are characteristic lesions of necrotic enteritis. germ-free chickens receiving either purified alpha-toxin or the supernatant of broth cultures of c perfringens died, but no bird died after receiving supernatant of broth cultures ... | 1988 | 2897708 |
| haemolytic patterns for presumptive identification of clostridium perfringens type c. | 1988 | 2897738 | |
| [microbiological research methods of drinking water regulation in west germany from 1986. suitability of the specifications of din 38411, part 7, for the detection of sulfite-reducing, spore-forming anaerobes (clostridia)]. | the drinking-water regulations of the federal republic of germany, from 22.05.1986, contains in paragraph 1 the instructions: "drinking-water must be free of pathogens", and further in paragraph 11, "responsibilities of the employer or other owner of a water supplying facility", include that: "the official authority may direct, that the employer...of a water supplying facility has to extend or has to cause to extend the microbiological examinations in order to determine, that...sulfite-reducing, ... | 1988 | 2897749 |
| clostridium spiroforme toxin is a binary toxin which adp-ribosylates cellular actin. | we have purified from clostridium spiroforme strain 246 an heterogeneous population of proteins (sa) ranging from 43 to 47 kilodaltons exhibiting adp-ribosyl transferase activity as do c. botulinum c2 toxin component i or the ia chain of c. perfringens e iota toxin. c. spiriforme sa had alone no activity upon injection in mice or inoculated to vero cells. when spiroforme adp ribosyl transferase were mixed with a trypsin activated protein (sb) separated from c. spiroforme bacterial supernatant, a ... | 1988 | 2897847 |
| a modified m-cp medium for enumerating clostridium perfringens from water samples. | medium m-cp, designed for the isolation of clostridium perfringens from water samples, contains indoxyl beta-d-glucoside, an expensive chemical that is present at a high concentration in this medium. the use of m-cp with three concentrations of indoxyl beta-d-glucoside was tested at 0, 60, and 600 mg/l. lowering the amount of indoxyl beta-d-glucoside to 60 mg/l (1/10 the recommended concentration) reduced the cost of this medium without affecting its sensitivity. | 1988 | 2897874 |
| [clostridium perfringens infection with pronounced hemolysis]. | 1988 | 2898093 | |
| egg yolk paste for determining some food poisoning bacteria. | egg yolk, aseptically prepared from fresh eggs, was partially dehydrated with a 40% high fructose corn syrup solution, and 10% salt was added. this salted yolk paste was added to mannitol salt agar for the detection of staphylococcus aureus, to nacl-glycine kim and goepfert medium for detection of bacillus cereus, to clostridium welchii agar for detection of c. perfringens, and to gifu anaerobic medium for detection of c. botulinum. these food poisoning bacteria showed the same lecithovitellin ( ... | 1988 | 2898139 |
| a new kinetic approach for studying phospholipase c (clostridium perfringens alpha toxin) activity on phospholipid monolayers. | the enzymatic activity of purified phospholipase c (alpha toxin) from clostridium perfringens was investigated with various phospholipid monolayers. a two-step reaction was used. enzymatic hydrolysis of insoluble lecithin films by phospholipase c, generating 1,2-diacylglycerol and water-soluble phosphocholine, was coupled with the action of pancreatic lipase in order to give rise to fatty acid and 2-monoacylglycerol, which are rapidly desorbed from the interface. with this new procedure, it is p ... | 1988 | 2898259 |
| early diagnosis of clostridial gas gangrene using sialidase antibodies. | in order to improve the diagnosis of gas gangrene, especially at an early stage of infection, new ways for the detection of the responsible clostridia were investigated. sialidase, known to be excreted in large amounts by the most frequently occurring myonecrotizing clostridial species, clostridium perfringens, clostridium septicum, and clostridium sordellii, was isolated. with polyclonal antibodies raised against these enzymes, two immunological assays were established, which are directed again ... | 1988 | 2898309 |
| simplified methods for the microbiological evaluation of bottled natural mineral waters. | the conventional methods for the microbiological examination of natural mineral water were compared with a simplified procedure. the results indicate that when indicator micro-organisms are present in water, they may not be detected in the simplified method. an alternative procedure, including the determination of pseudomonas aeruginosa, is suggested. | 1988 | 2898469 |
| phospholipase c from clostridium perfringens induces human platelet aggregation in plasma. | we studied the aggregating effect of different concentrations of phospholipase c (plc) (extracted from clostridium perfringens) on human platelet-rich plasma (prp). prp was preincubated with plc for 3 min at 37 degrees c and the platelet aggregation was followed for 10 min. the threshold aggregating concentration (tac) of plc was 3-4 u/ml. we also studied the potentiation of plc with other stimuli on platelet aggregation. potentiating stimuli, such as arachidonic acid (aa), adp. platelet activat ... | 1988 | 2898818 |
| growth of clostridium perfringens in cooked chili during cooling. | u.s. department of agriculture regulations require that brick chili be cooled from 48.9 degrees c to 4.4 degrees c within 2 h of cooking, but processors may not always be able to comply. studies were conducted to evaluate the extent of bacterial multiplication resulting from outgrowth of germinated clostridium perfringens spores experimentally inoculated into chili and incubated at various temperatures. inoculated samples were heated (75 degrees c for 20 min) to activate spores, quickly equilibr ... | 1988 | 2898919 |
| microbiological evaluation of a hospital delivered meals service using precooked chilled foods. | a delivered meals service supplying centrally produced, precooked, chilled foods to 24 hospitals was introduced in plymouth health district between august 1985 and july 1986. over 18 months, 3393 food items were examined microbiologically, using the criteria recommended by the department of health and social security (dhss) (1980). no salmonella spp., staphylococcus aureus or clostridium perfringens were detected. seventy-five (8.6%) of 876 cooked vegetable items had total viable counts (tvc) gr ... | 1988 | 2899105 |
| cefotaxime lavage in children undergoing appendicectomy. | in an attempt to reduce postoperative sepsis, a series of randomised, double-blind studies was begun in 1982, using cefotaxime as backbone therapy. up to 1985 (stages i and ii), the best results were obtained using a combination of cefotaxime (75 mg/kg intravenously in 3 doses at 12-hourly intervals) plus metronidazole (10 mg/kg intravenously in 3 doses), both drugs administered 1 hour before surgery (preoperatively) or at anaesthetic induction (peroperatively). in 300 consecutive cases, the wou ... | 1988 | 2899496 |
| clostridial septic arthritis: case report and review of the literature. | we describe a patient who had septic arthritis caused by clostridium perfringens. clostridial organisms are very uncommon causes of septic arthritis. only 13 cases have been reported previously. the diagnosis should be suspected in patients with a history of penetrating joint trauma and in immunocompromised patients. successful treatment has usually consisted of surgical synovectomy in combination with high-dose intravenous penicillin therapy. multiple aspirations of affected joints as a definit ... | 1988 | 2894833 |
| hematogenously acquired infection of a total knee arthroplasty by clostridium perfringens. | a 64-year-old man with total knee arthroplasty for tricompartmental osteoarthritis had a postoperative course complicated by an attack of acute cholecystitis and was treated with cholecystectomy. the tka was hematogenously seeded with clostridium perfringens, which necessitated emergency removal of the implants, debridement, and ultimately arthrodesis. | 1988 | 2894912 |
| the passive protection of lambs against clostridium perfringens type d with semi-purified hyperimmune serum. | weaned lambs, having a detectable level of maternal antibodies (1-2 units/ml) against c. perfringens type d, showed protective antitoxin levels lasting for 29 days after receiving a single parenteral dose of 200 units/kg hyperimmune serum. lambs, having no maternal antibodies (less than 0,07 units/ml) to c. perfringens type d but receiving the same dose of hyperimmune serum, maintained protective antibody levels for only 21 days. three weeks after the titres fell below the minimum protective lev ... | 1988 | 2895443 |
| clostridial cystitis in a nondiabetic man: case report. | 1988 | 2895555 | |
| effect of perfringolysin o on the lateral diffusion constant of membrane proteins of hepatocytes as revealed by fluorescence recovery after photobleaching. | perfringolysin o is a thiol-activated cytolytic exotoxin the primary receptor of which is the membrane cholesterol on the cell surface. the effect of perfringolysin o was tested in various hepatocyte preparations. (i) smears of fresh liver exposed to a mild h2o2 (1.0 mm) injury for 10 min at 37 degrees c, develop a 'peroxide-induced autofluorescence' (piaf) on the membrane proteins. piaf is suitable for measuring the average lateral diffusion constant (d) of the membrane proteins by means of flu ... | 1988 | 2895669 |
| characterization of an outbreak of clostridium perfringens food poisoning by quantitative fecal culture and fecal enterotoxin measurement. | published criteria for implicating clostridium perfringens as the cause of food-poisoning outbreaks include finding a median fecal c. perfringens spore count of greater than 10(6)/g among specimens from ill persons. we investigated a food-poisoning outbreak with the epidemiologic characteristics of c. perfringens-related disease in a nursing home in which the median fecal spore count for ill patients (2.5 x 10(7)/g) was similar to that for well patients (4.0 x 10(6)/g), making the etiology of th ... | 1988 | 2895776 |
| transformation of clostridium perfringens l forms with shuttle plasmid dna. | l-form (l-phase) cultures of clostridium perfringens were tested for their transformability with plasmid dna. three l-form strains were transformable, but one, strain l-13, was superior to the others. this strain was easily and reproducibly transformed with previously described shuttle vectors which were derived from either c. perfringens or escherichia coli. strain l-13 was transformable by a variety of methods, and a new micromethod worked well under both aerobic and anaerobic conditions. the ... | 1988 | 2894199 |
| development of a new shuttle plasmid system for escherichia coli and clostridium perfringens. | we constructed a 7.9-kilobase-pair recombinant shuttle plasmid, designated phr106, by combining desired segments of three plasmids: an escherichia coli plasmid (psl100) which provides a multiple cloning site, a clostridium perfringens plasmid (pju122) which provides a clostridial origin of replication, and an e. coli plasmid (pjir62) which provides an e. coli origin of replication, an ampicillin resistance gene, and a chloramphenicol resistance gene of clostridial origin. the shuttle plasmid tra ... | 1988 | 2894200 |
| experimental induction of abdominal tympany, abomasitis, and abomasal ulceration by intraruminal inoculation of clostridium perfringens type a in neonatal calves. | the etiologic role of clostridum perfringens type a in the acute abdominal syndrome characterized by abomasal and rumen tympany, abomasitis, and abomasal ulceration was investigated in neonatal calves. eight calves, 4 to 12 days old, were inoculated intraruminally with toxigenic c perfringens type a. before and after c perfringens inoculation, blood samples were collected from all calves for blood gas and serum biochemical analysis and for determination of serum copper concentration; ruminal flu ... | 1988 | 2894790 |
| adp-ribosylation of skeletal muscle and non-muscle actin by clostridium perfringens iota toxin. | the enzymatically active component ia of clostridium perfringens iota toxin adp-ribosylated actin in human platelet cytosol and purified platelet beta/gamma-actin, in a similar way to that been reported for component i of botulinum c2 toxin. adp-ribosylation of cytosolic and purified actin by either toxin was inhibited by 0.1 mm phalloidin indicating that monomeric g-actin but not polymerized f-actin was the toxin substrate. perfringens iota toxin and botulinum c2 toxin were not additive in adp- ... | 1988 | 2892681 |
| a large clostridium perfringens foodborne outbreak with an unusual attack rate pattern. | on november 7, 1985, a clostridium perfringens gastroenteritis outbreak occurred in approximately 44% of the 1,362 employees at a connecticut factory. although the same foods were served to all three shifts at an employee banquet on november 6, the attack rate was almost twice as high for those who ate on the day shift (attack rate = 50%) than for those on the evening shift (attack rate = 20%) or night shift (attack rate = 29%). among employees of the day shift, attack rates were highest for tho ... | 1988 | 2893540 |
| alkyl-linked diquinolines are monofunctional at-selective dna-intercalating agents. | the binding of a homologous series of alkyl-linked 4-aminodiquinolines to circular and linear dnas was studied using viscometric titrations and equilibrium dialysis. the compounds are monofunctional intercalators with the capacity for intercalative binding reaching a peak for the heptane homologue. they show marked at-base pair selectivity, which suggests that the non-intercalated quinoline ring may lie in the dna minor groove. affinities for calf thymus dna increase as the alkyl chain is length ... | 1988 | 2893746 |
| lethal effects and cardiovascular effects of purified alpha- and theta-toxins from clostridium perfringens. | shock, a common and frequently fatal manifestation of gas gangrene caused by clostridium perfringens, is probably mediated by extracellular toxins. previous studies implicating alpha-toxin as the major lethal factor were frequently done with preparations contaminated with a second lethal factor, theta-toxin. we purified alpha- and theta-toxins from c. perfringens and demonstrated that both were lethal to mice. we investigated the effects of these purified toxins on cardiovascular function in int ... | 1988 | 2891775 |
| infectious diarrhea due to clostridium perfringens. | 1988 | 2891779 | |
| release of proteins from the surface of bovine central nervous system myelin by salts and phospholipases. | incubation of bovine cns myelin with phospholipase c from bacillus cereus under conditions that lead to extensive phospholipid degradation caused 10% of the myelin protein to be released from the membrane. the myelin basic protein (mbp) was a major component of the dissolved protein. comparable incubations with phospholipase c from clostridium perfringens, phosphatidylinositol-specific phospholipase c from staphylococcus aureus, or cabbage phospholipase d removed little mbp. however, concentrati ... | 1988 | 2448423 |
| adsorption of llcmk2 cell-grown sendai virus onto human red blood cells and its release from the virus adsorbed cells. | an early stage of virus adsorption was studied in a system of sendai virus metabolically labeled with [3h]leucine in llcmk2 cells and of human red blood cells (rbcs). the efficiency of viral release from the virus-bound rbcs by incubation at 37 c depended on the number of virus particles which had been used for adsorption onto the rbc at 4 c. when 7.8 x 10(2) virus particles were previously adsorbed onto the rbc at 4 c, most of the viruses were dissociated from the rbc at 37 c. in the case of ad ... | 1988 | 2853288 |
| clinical evaluation of the vitek ani card for identification of anaerobic bacteria. | an evaluation of the vitek anaerobe identification (ani) card was performed with 341 bacterial isolates, including 313 clinical isolates and 28 stock strains of anaerobic microorganisms. identifications obtained with the ani card were compared with those determined by conventional methods. the card identified 73.2% of 149 anaerobic gram-negative bacilli, 63.6% of 44 clostridium spp., 65.8% of 38 anaerobic nonsporeforming gram-positive bacilli, and 69.1% of 110 anaerobic cocci, with no further te ... | 1988 | 3343321 |
| beta-glucuronidase activity related to bacterial growth in common bile duct bile in gallstone patients. | beta-glucuronidase activity in the bile may be of importance in the etiology of pigment gallstones. this enzyme is of hepatic or bacterial origin. we have described a method to measure the activity of bacterial beta-glucuronidase in human bile, using 4-nitrophenyl-beta-d-glucopyranosiduronic acid as substrate. the method was used to measure the beta-glucuronidase activity in the bile from 51 patients with gallstone disease. this activity was related to the presence of beta-glucuronidase-producin ... | 1988 | 3344403 |
| characterization of the dermal lesions induced by a purified protein from toxigenic pasteurella multocida. | the dermonecrotic effect of purified pasteurella multocida toxin (pmt) was studied sequentially in guinea pigs and rats. the skin reaction was initially an acute inflammatory reaction, with edema and emigration of neutrophils and a few eosinophils and diapedesis of some erythrocytes. four hours after intracutaneous injection the vessels were congested and thrombocytes were focally attached to the endothelial wall. twenty-four h after the injection the inflammatory reaction appeared more severe a ... | 1988 | 3345248 |
| spontaneous gas gangrene at a site of remote injury--localization due to circulating antitoxin. | 1988 | 3348030 |