Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| inhibition of human natural killer cell activity by cereulide, an emetic toxin from bacillus cereus. | the lipophilic toxin, cereulide, emitted by emetic food poisoning causing strains of bacillus cereus, is a powerful mitochondria toxin. it is highly lipophilic and rapidly absorbed from the gut into the bloodstream. we tested how this toxin influences natural killer (nk) cells, which are important effectors in defence against infections and malignancy. cereulide inhibited cytotoxicity and cytokine production of natural killer cells, caused swelling of natural killer cell mitochondria, and eventu ... | 2002 | 12197882 |
| a cell-cell signaling peptide activates the plcr virulence regulon in bacteria of the bacillus cereus group. | plcr is a pleiotropic regulator that activates the expression of genes encoding various virulence factors, such as phospholipases c, proteases and hemolysins, in bacillus thuringiensis and bacillus cereus. here we show that the activation mechanism is under the control of a small peptide: papr. the papr gene belongs to the plcr regulon and is located 70 bp downstream from plcr. it encodes a 48-amino-acid peptide. disruption of the papr gene abolished expression of the plcr regulon, resulting in ... | 2002 | 12198157 |
| simultaneous multiple substrate tag detection with esi-ion trap ms for in vivo bacterial enzyme activity profiling. | a bacterial identification method in which multiple enzyme activities are measured simultaneously and in vivo with electrospray ionization-mass spectrometry (esi-ms) is described. whole-cell bacteria are immobilized onto a filter support and incubated with a mixture of substrates. each substrate is chosen to measure a specific enzyme activity of a targeted bacterium and to produce a tag of unique molecular weight. after a predetermined incubation time, the solution is filtered, and the supernata ... | 2002 | 12199605 |
| structural determinants and hydrogen-bond network of the mononuclear zinc(ii)-beta-lactamase active site. | zinc(ii)-beta-lactamases are among the latest generation of antibiotic-resistant enzymes developed by bacteria against beta-lactams. here we have used density functional theory to provide the full structure of the catalytic site from bacillus cereus mononuclear beta-lactamase ii. calculations are carried out on relative large models built on the x-ray structure of the free enzyme at the highest available resolution (1.7 a, pdb entry 3bc2). the most stable conformation emerging from our calculati ... | 2002 | 12203007 |
| successful non-surgical treatment of brain abscess and necrotizing fasciitis caused by bacillus cereus. | musculoskeletal and central nervous system infections caused by bacillus cereus are very rare. only a few cases have been reported, whose clinical courses strongly suggested that surgical procedures combined with appropriate antimicrobial therapy are necessary to cure these infections. a 60-year-old man with severe neutropenia due to myelodysplastic syndrome, developing necrotizing fasciitis and brain abscess caused by bacillus cereus is reported. without performing any surgical procedures, the ... | 2002 | 12211541 |
| chemical constituents and antimicrobial activity of the essential oil of lantana xenica. | the aim of this work was to evaluate the chemical composition of lantana xenica essential oil and its antimicrobial activity. the oil from the aerial parts of lantana xenica mold. (verbenacea) was obtained by steam distillation and analyzed by gas chromatography/mass spectrometry. the major constituent of the oil was (e)-caryophyllene (35.2 %), with minor amounts of gamma-cadinene (13.3 %), alpha-pinene (9.3 %), ocimene (9.2 %) and germacrene d (6.6 %). the antimicrobial assays showed that the e ... | 2002 | 12221608 |
| relapsing bacillus cereus peritonitis in two patients on peritoneal dialysis. | 2002 | 12227407 | |
| single-cell protein production from ram horn hydrolysate by bacteria. | ram horns obtained from the slaughterhouse of erzurum, turkey were hydrolyzed by treating with acid (6n-hcl) and ram horn hydrolysate (rhh) was obtained. the hydrolysate was used as substrate to grow bacillus cereus nrrl b-3711, bacillus subtilis nrrl nrs-744 and escherichia coli in batch system at 30 degrees c; air 1.5 v/v/min; stirring 150 rpm. both rhh and biomass samples were analyzed. the bacterial cells produced in this hydrolysate were found to be rich in total protein (66%, 68% and 71% f ... | 2002 | 12227535 |
| bacillus thuringiensis in fecal samples from greenhouse workers after exposure to b. thuringiensis-based pesticides. | in a study of occupational exposure to bacillus thuringiensis, 20 exposed greenhouse workers were examined for bacillus cereus-like bacteria in fecal samples and on biomonitoring filters. bacteria with the following characteristics were isolated from eight individuals: intracellular crystalline inclusions characteristic of b. thuringiensis, genes for and production of b. cereus enterotoxins, and positivity for cry11 as determined by pcr. dna fingerprints of the fecal isolates were identical to t ... | 2002 | 12324337 |
| diversity of endophytic bacterial populations and their interaction with xylella fastidiosa in citrus plants. | citrus variegated chlorosis (cvc) is caused by xylella fastidiosa, a phytopathogenic bacterium that can infect all citrus sinensis cultivars. the endophytic bacterial communities of healthy, resistant, and cvc-affected citrus plants were studied by using cultivation as well as cultivation-independent techniques. the endophytic communities were assessed in surface-disinfected citrus branches by plating and denaturing gradient gel electrophoresis (dgge). dominant isolates were characterized by fat ... | 2002 | 12324338 |
| the identification of a tetracycline resistance gene tet(m), on a tn916-like transposon, in the bacillus cereus group. | in order to investigate whether resistance genes present in bacteria in manure could transfer to indigenous soil bacteria, resistant isolates belonging to the bacillus cereus group (bacillus cereus, bacillus anthracis and bacillus thuringiensis) were isolated from farm soil (72 isolates) and manure (12 isolates) samples. these isolates were screened for tetracycline resistance genes (tet(k), tet(l), tet(m), tet(o), tet(s) and tet(t)). of 88 isolates examined, three (3.4%) isolates carried both t ... | 2002 | 12351239 |
| an outbreak of bacillus cereus implicating a part-time banquet caterer. | in the aftermath of a party, 70% (25 of 36) of attendees had gastroenteritis. the objectives of this study were to identify a risk factor associated with the food during the banquet and to identify measures of control for avoiding this kind of outbreak in the future. | 2002 | 12353456 |
| isolation of antimicrobial compounds from guava (psidium guajava l.) and their structural elucidation. | four antibacterial compounds were isolated from leaves of guava (psidium guajava l.), and the structures of these compounds were established on the basis of chemical and spectroscopic evidence. two new flavonoid glycosides, morin-3-o-alpha-l-lyxopyranoside and morin-3-o-alpha-l-arabopyranoside, and two known flavonoids, guaijavarin and quercetin, were identified. the minimum inhibition concentration of morin-3-o-alpha-l-lyxopyranoside and morin-3-o-alpha-l-arabopyranoside was 200 microg/ml for e ... | 2002 | 12353635 |
| brain abscesses resulting from bacillus cereus and an aspergillus-like mold. | an 11-year-old boy with alveolar rhabdomyosarcoma of the thigh experienced three instances of catheter-related bacteremia resulting from after two episodes of seizures, two low-density lesions in the right parietal lobe and the left corpus callosum with enhanced pericavitary opacity were detected. the catheter was removed. a brain biopsy sample grew and revealed dichotomously branched septate hyphae compatible with the patient was treated with ceftriaxone and liposomal amphotericin b for 12 and ... | 2002 | 12368698 |
| development of a laser-induced cell lysis system. | a novel cell lysis system was developed that is based on laser-induced disruption of bacterial and yeast cells. it will find application as a rapid, efficient and clean sample preparation step in bioanalytical detection systems. using e. coli as our model analyte, we optimized cell lysis with respect to optimal laser wavelength, lowest energy input requirements, rna release from the cells, and potential protein damage. the optimized system was finally applied to the lysis of four additional micr ... | 2002 | 12373389 |
| effective use of nisin to control bacillus and clostridium spoilage of a pasteurized mashed potato product. | heat-resistant spore-forming bacteria such as bacillus and clostridium can survive and grow in cooked potato products. this situation represents both a public health problem and an economic problem. the natural food preservative nisin is used in heat-treated foods to prevent the growth of such bacteria. a cocktail of clostridium sporogenes and clostridium tyrobutyricum spores was inoculated into cooked mashed potatoes, which were vacuum packed, pasteurized, and incubated at 8 and 25 degrees c. t ... | 2002 | 12380742 |
| adhesion of bacillus spores and escherichia coli cells to inert surfaces: role of surface hydrophobicity. | the ability of bacterial spores and vegetative cells to adhere to inert surfaces was investigated by means of the number of adherent spores (bacillus cereus and bacillus subtilis spores) and escherichia coli cells and their resistance to cleaning or rinsing procedures (adhesion strength). six materials (glass, stainless steel, polyethylene high density (pehd), polyamide-6, polyvinyl chloride, and teflon) were tested. slight differences in the number of adherent spores (less than 1 log unit) were ... | 2002 | 12381029 |
| lethal factor to mice produced by escherichia coli isolated from chickens with swollen head syndrome. | a lethal toxin similar to bacillus cererus lethal toxin was detected in the culture supernatants of escherichia coli isolated from chickens with swollen head syndrome. the lethal activity was heat-labile, protease-sensitive and killed mice within 10 min. the light microscopy of the histopathological studies revealed that the principal organ affected by this toxin was the lung but the liver and kidneys also showed lesions. the relevance of this lethal activity from e. coli remains to be determine ... | 2002 | 12516774 |
| presence of erm genes among macrolide-resistant gram-positive bacteria isolated from danish farm soil. | the presence of erm genes was investigated among macrolide-resistant gram-positive bacteria isolated from soil samples collected from four danish farms that had been treated with animal waste. soil samples were collected before, a few days after spread and 1 1/2 months and 5 months later. in 33% (9/27) of these isolates, an erm gene was detected using pcr. eight isolates were positive for erm(b) and one isolate was positive for erm(c). no isolates contained erm(a), erm(d) or erm(f). the positive ... | 2002 | 12503914 |
| dna sequence conservation between the bacillus anthracis pxo2 plasmid and genomic sequence from closely related bacteria. | complete sequencing and annotation of the 96.2 kb bacillus anthracis plasmid, pxo2, predicted 85 open reading frames (orfs). bacillus cereus and bacillus thuringiensis isolates that ranged in genomic similarity to b. anthracis, as determined by amplified fragment length polymorphism (aflp) analysis, were examined by pcr for the presence of sequences similar to 47 pxo2 orfs. | 2002 | 12473162 |
| role of the c-domain in the biological activities of clostridium perfringens alpha-toxin. | clostridium perfringens alpha-toxin (370 residues) possesses hemolytic and lethal activities as well as the enzymatic activity of phospholipase c (plc). in this study we examined the role of the c-domain (251-370 residues; cp251- 370) in biological activities of the toxin. the n-domain (1-250 residues; cp1- 250) of the alpha-toxin as well as the bacillus cereus phospholipase c (bcplc) possessed plc activity, but did not bind to rabbit erythrocytes and lyse them. a hybrid protein (bc-cp251-370) c ... | 2002 | 12477243 |
| antibacterial activity of a stearic acid derivative from stemodia foliosa. | from the hexane-soluble fraction of an ethanol extract from leaves and stems of stemodia foliosa (scrophulariaceae), the new stearic acid 4-[(n-pentoxy)phenethyl] ester (1) was isolated. this compound exhibited antibacterial properties at 10 microg/ml concentration by using disc diffusion method against gram-positive bacteria bacillus cereus and bacillus subtilis and fast-acid bacterium mycobacterium fortuitum. the structure of the new compound was elucidated by spectroscopic methods and by chem ... | 2002 | 12494347 |
| effect of dissolved carbon dioxide on thermal inactivation of microorganisms in milk. | postpasteurization addition of co2 inhibits growth of certain microorganisms in dairy products, but few workers have investigated the effect of co2 on the thermal inactivation of microorganisms during pasteurization. concentrations of co2 ranging from 44 to 58 mm added to raw whole milk significantly (p < 0.05) reduced the number of surviving standard plate count (spc) organisms in milk heated over the range of 67 to 93 degrees c. a decrease in thermal survival rates (d-values) for pseudomonas f ... | 2002 | 12495011 |
| altered ability of bacillus cereus spores to grow under unfavorable conditions (presence of nisin, low temperature, acidic ph, presence of nacl) following heat treatment during sporulation. | the effect of thermal treatment on the heat resistance of bacillus cereus spores and their ability to germinate and grow under more or less adverse conditions during sporulation was investigated. spores produced by sporulating cells subjected to a mild heat treatment (at a temperature 15 degrees c higher than the growth temperature) were more resistant to heat than were spores produced by untreated cells. spore germination and growth (the lag time, the maximal growth rate, and the occurrence of ... | 2002 | 12495012 |
| bidirectional control of sphingomyelinase activity and surface topography in lipid monolayers. | lipid lateral organization is increasingly found to modulate membrane-bound enzymes. we followed in real time the reaction course of sphingomyelin (sm) degradation by bacillus cereus sphingomyelinase (smase) of lipid monolayers by epifluorescence microscopy. there is evidence that formation of ceramide (cer), a lipid second messenger, drives structural reorganization of membrane lipids. our results provide visual evidence that smase activity initially alters surface topography by inducing phase ... | 2002 | 12496108 |
| acid tolerance response is low-ph and late-stationary growth phase inducible in bacillus cereus tz415. | the acid tolerance of foodborne pathogen bacillus cereus tz415 was examined. b. cereus was more tolerant to an acid challenge at ph 4.0 when cells were grown at low ph in regulated batch cultures of rich j broth (jb) medium. the ph-inducible acid tolerance response (atr) was maximal at ph 5.0, a sublethal growth condition inducing a remarkable cell elongation. during growth at regulated ph 7.0 and 6.0, b. cereus tz415 became more acid sensitive from lag to stationary growth phase and the acid to ... | 2002 | 12382686 |
| detecting bacteria in platelet concentrates by use of reagent strips. | iatrogenic infection of immunosuppressed or immunocompromised hosts secondary to receipt of blood components containing bacteria may result in serious adverse outcomes. measurement of ph and glucose by use of inexpensive reagent strips has been proposed as a practical means of screening for bacteria in platelet concentrate (pc) units. | 2002 | 12385414 |
| pathogenic potential of fifty bacillus weihenstephanensis strains. | the aim of this study was to evaluate the food poisoning potential of strains of the new species in the bacillus cereus group, b. weihenstephanensis. fifty strains were tested for cytotoxicity in a vero cell assay, and 23 of the strains were also tested for production of enterotoxin components with commercial antibody kits, and for presence of enterotoxin gene components by polymerase chain reaction (pcr). the majority of the strains (72%) were not cytotoxic, although all of the strains that wer ... | 2002 | 12393199 |
| sequencing of 16s rrna gene: a rapid tool for identification of bacillus anthracis. | in a bioterrorism event, a tool is needed to rapidly differentiate bacillus anthracis from other closely related spore-forming bacillus species. during the recent outbreak of bioterrorism-associated anthrax, we sequenced the 16s rrna generom these species to evaluate the potential of 16s rrna gene sequencing as a diagnostic tool. we found eight distinct 16s types among all 107 16s rrna gene seqs fuences that differed from each other at 1 to 8 positions (0.06% to 0.5%). all 86 b. anthracis had an ... | 2002 | 12396926 |
| a novel class of microbial phosphocholine-specific phospholipases c. | in this report we describe the 1,500-fold purification and characterization of the haemolytic phospholipase c (plc) of pseudomonas aeruginosa, the paradigm member of a novel plc/phosphatase superfamily. members include proteins from mycobacterium tuberculosis, bordetella spp., francisella tularensis and burkholderia pseudomallei. purification involved overexpression of the plchr1,2 operon, ion exchange chromatography and native preparative polyacrylamide gel electrophoresis. matrix-assisted lase ... | 2002 | 12410824 |
| kinetic evidence for a substrate-induced fit in phosphonoacetaldehyde hydrolase catalysis. | phosphonoacetaldehyde hydrolase (phosphonatase) from bacillus cereus catalyzes hydrolytic p-c bond cleavage of phosphonoacetaldehyde (pald) via a schiff base intermediate formed with lys53. a single turnover requires binding of pald to the active site of the core domain, closure of the cap domain containing the lys53 over the core domain, and dissociation of the products following catalysis. the ligand binding and dissociation steps occur from the "open conformer" (domains are separated and the ... | 2002 | 12416981 |
| a novel class of self-sufficient cytochrome p450 monooxygenases in prokaryotes. | the bacillus cytochrome p450 bm3 integrates an entire p450 system in one polypeptide and represents a convenient prokaryotic model for microsomal p450s. this self-sufficient class ii p450 is also present in actinomycetes and fungi. by genome analysis we have identified additional homologues in the pathogenic species bacillus anthracis and bacillus cereus, and in ralstonia metallidurans. this analysis also revealed a novel class of putative self-sufficient p450s, p450 pfor, comprising a class i p ... | 2002 | 12419614 |
| daptomycin morphostructural damage in bacillus cereus visualized by atomic force microscopy. | daptomycin is a novel, rapidly bactericidal in vitro antibiotic that is under investigation for the treatment of serious gram-positive infections. although daptomycin appears to disrupt membrane function, the precise mechanism of action has not been fully elucidated. atomic force microscopy (afm) is an innovative technique that allows high-resolution visualization and digital image manipulation of cell surface structures in 3 dimensions without the use of photons and electrons. the aim of this s ... | 2002 | 12420849 |
| colony shape as a genetic trait in the pattern-forming bacillus mycoides. | bacillus mycoides flügge, a gram-positive, non-motile soil bacterium assigned to bacillus cereus group, grows on agar as chains of cells linked end to end, forming radial filaments curving clock- or counter-clockwise (sin or dx morphotypes). the molecular mechanism causing asymmetric curving is not known: our working hypothesis considers regulation of filamentous growth as the prerequisite for these morphotypes. | 2002 | 12429070 |
| magnetized carbonyl iron and insoluble zirconium hydroxide mixture facilitates bacterial concentration and separation from nonfat dry milk. | a mixture of magnetized carbonyl iron and insoluble zirconium hydroxide was investigated for its ability to concentrate various foodborne pathogens from 25-ml samples of reconstituted nonfat dry milk. each sample was artificially contaminated with 10(3) to 10(6) cfu/25 ml of representative foodborne pathogens (salmonella enterica serovar enteritidis, listeria monocytogenes, and bacillus cereus spores) and processed for bacterial concentration with high-speed centrifugation for the primary concen ... | 2002 | 12430708 |
| search for potential vaccine candidate open reading frames in the bacillus anthracis virulence plasmid pxo1: in silico and in vitro screening. | a genomic analysis of the bacillus anthracis virulence plasmid pxo1, aimed at identifying potential vaccine candidates and virulence-related genes, was carried out. the 143 previously defined open reading frames (orfs) (r. t. okinaka, k. cloud, o. hampton, a. r. hoffmaster, k. k. hill, p. keim, t. m. koehler, g. lamke, s. kumano, j. mahillon, d. manter, y. martinez, d. ricke, r. svensson, and p. j. jackson, j. bacteriol. 181:6509-6515, 1999) were subjected to extensive sequence similarity search ... | 2002 | 12438358 |
| the enterotoxin t (bcet) from bacillus cereus can probably not contribute to food poisoning. | a polymerase chain reaction (pcr) fragment from strain nvh 38 (containing bcet) was cloned, sequenced and expressed in escherichia coli. this sequence showed 50-60% identity to the original. when this bcet clone was expressed in e. coli no biological activity was found in either supernatants or cell extracts. cell extracts from the bacillus cereus strains (nvh 38 and b-4ac) were also negative on vero cells. neutralisation of supernatant from b. cereus b-4ac using a monoclonal antibody (reacting ... | 2002 | 12445654 |
| amplified fragment length polymorphism (aflp) analysis of clostridium novyi, c. perfringens and bacillus cereus isolated from injecting drug users during 2000. | as part of the follow-up investigations associated with an outbreak of severe illness and death among illegal injecting drug users during 2000, 43 cultures of clostridium novyi type a, 40 c. perfringens type a and 6 isolates of bacillus cereus were characterised by amplified fragment length polymorphism (aflp) analysis. among the 43 c. novyi isolates, 23 different aflp profiles were detected. the same aflp profile was detected in isolates from 18 drug users investigated during 2000 from scotland ... | 2002 | 12448684 |
| an investigation into the microflora of heroin. | in 2000, an unusual increase of morbidity and mortality among illegal injecting drug users in the uk and ireland was reported and clostridium novyi was identified as the likely source of the serious infection, although infections due to c. botulinum and bacillus cereus were also reported. because heroin was a possibile source of infection, this study investigated the microflora of heroin samples seized in england during 2000 and 2002. two methods were developed for the examination of the microfl ... | 2002 | 12448685 |
| toward an understanding of microbial communities through analysis of communication networks. | bacteria receive signals from diverse members of their biotic environment. they sense their own species through the process of quorum sensing, which detects the density of bacterial cells and regulates functions such as bioluminescence, virulence, and competence. bacteria also respond to the presence of other microorganisms and eukaryotic hosts. most studies of microbial communication focus on signaling between the microbe and one other organism for empirical simplicity and because few experimen ... | 2002 | 12448752 |
| broadband 10-300 ghz stimulus-response sensing for chemical and biological entities. | by illuminating the sample with a broadband 10-300 ghz stimulus and coherently detecting the response, we obtain reflection and transmission spectra of common powdered substances, and compare them as a starting point for distinguishing concealed threats in envelopes and on personnel. because these samples are irregular and their dielectric properties cannot be modulated, however, the spectral information we obtain is largely qualitative. to show how to gain quantitative information on biological ... | 2002 | 12452568 |
| genetic relationships of bacillus anthracis and closely related species based on variable-number tandem repeat analysis and box-pcr genomic fingerprinting. | variable-number tandem repeats (vntr) analysis and box-repeat-based pcr (box-pcr) genomic fingerprinting were performed on 25 bacillus strains to investigate the genetic relatedness of bacillus anthracis to the closely related species. based on vntr analysis, all b. anthracis strains could be assigned to (vntr)(4), which is the most commonly found type in the world. interestingly, a (vntr)(2) was also observed in bacillus cereus kctc 1661 and with an exact match to the tandem repeats found in b. ... | 2002 | 11886745 |
| gas sensor arrays for early detection of infection in mammalian cell culture. | the detection of bacterial infections in a mammalian cell culture process is realised using a gas sensor array. in production-scale and laboratory-scale cultivations of a perfused recombinant cho-cell culture producing human blood coagulation factor viii, we show that the gas sensor array identifies bacterial contamination earlier than conventional methods. the sensitivity of the instrument is verified by inoculation of a blank cell culture medium with defined bacterial cell counts. | 2002 | 11888730 |
| antimicrobial synergistic effect of linolenic acid and monoglyceride against bacillus cereus and staphylococcus aureus. | the antimicrobial effect of linolenic acid with or without monoglyceride (glycerol laurate or glycerol myristate) against six food-borne microorganisms was determined in broth medium. minimum inhibitory concentration of linolenic acid on bacillus cereus and staphylococcus aureus was 20 and 50 ppm, respectively. the growth of b. cereus treated with linolenic acid at 10 ppm with 10 ppm monoglyceride was more inhibitory than that of linolenic acid alone, and the viable cell population was reduced 2 ... | 2002 | 11902977 |
| influence of the probiotic strain bacillus cereus var. toyoi on the development of enterobacterial growth and on selected parameters of bacterial metabolism in digesta samples of piglets. | the effect of the probiotic strain bacillus cereus var. toyoi on enterobacterial growth and selected metabolic parameters in digesta samples from piglets before and shortly after weaning was studied. growth capacities of enterobacteria in digesta samples were significantly reduced, but no correlation was found between enterobacterial growth and concentrations of short-chain fatty acids and lactic acid, which are often influenced by other probiotic preparations. tendencies for higher amounts of s ... | 2002 | 11906572 |
| [comparative study of the elemental composition of vegetative and dormant microbial cells]. | x-ray microanalysis showed that vegetative cells, viable resting forms, and nonviable forms (micromummies) of the bacteria bacillus cereus and micrococcus luteus and the yeast saccharomyces cerevisiae differ in the contents of bioelements s, p, ca, and k and the ca/k and p/s ratios. viable resting forms (cystlike refractory cells and bacillar endospores) had more calcium and less phosphorus and potassium than vegetative cells, the difference being higher for bacilli than for micrococci and yeast ... | 2002 | 11910805 |
| rheological characteristics of microbial suspensions of pseudomonas aeruginosa and bacillus cereus. | the rheological properties of the bacteria pseudomonas aeruginosa and bacillus cereus have been investigated. the apparent viscosity of the bacterial suspensions has been measured at different conditions. the results showed that the bacterial suspensions' apparent viscosity increased with increasing biomass concentration of each of these strains. the p. aeruginosa suspension followed shear thinning behavior while b. cereus suspension followed shear thickening behavior. the shear stress versus sh ... | 2002 | 11911895 |
| role of tryptophan residues in interfacial binding of phosphatidylinositol-specific phospholipase c. | the phosphatidylinositol-specific phospholipase c (pi-plc) from bacillus thuringiensis exhibits several types of interfacial activation. in the crystal structure of the closely related bacillus cereus pi-plc, the rim of the active site is flanked by a short helix b and a loop that show an unusual clustering of hydrophobic amino acids. two of the seven tryptophans in pi-plc are among the exposed residues. to test the importance of these residues in substrate and activator binding, we prepared sev ... | 2002 | 11912206 |
| effect of carbon dioxide on the growth of bacillus cereus spores in milk during storage. | the effects of the addition of 11.9 mm co2 on the growth of bacillus cereus spores inoculated into sterile homogenized whole milk at 101 and 106 spores/ml and stored at 6.1 degrees c, was examined weekly for 35 d. colony-forming units from co2 treated inoculated milks decreased over 35 d at a rate similar to that of untreated inoculated milk, as defined by linear regression. plate counts for treated and control milks inoculated at 10(1) cfu/ml were not significantly different on sampling d 0, 14 ... | 2002 | 11860107 |
| a mouse serine protease tesp5 is selectively included into lipid rafts of sperm membrane presumably as a glycosylphosphatidylinositol-anchored protein. | we have previously indicated that at least in mouse, sperm serine protease(s) other than acrosin probably act on the limited proteolysis of egg zona pellucida to create a penetration pathway for motile sperm, although the participation of acrosin cannot be ruled out completely. a 42-kda gelatin-hydrolyzing serine protease present in mouse sperm is a candidate enzyme involved in the sperm penetration of the zona pellucida. in this study, we have pcr-amplified an est clone encoding a testicular se ... | 2002 | 11861648 |
| cloning and overexpression of bacillus cereus penicillin-binding protein 3 gene in escherichia coli. | the pbp3 gene encoding pbp3 of bacillus cereus was cloned and sequenced. for this purpose, pbp3 was first purified from b. cereus ts-4, and n-terminal amino acid sequences of the peptides obtained from the protease digests of the protein were analyzed. the b. cereus ts-4 pbp3 gene consisted of an open reading frame of 1,986 bp encoding 662 amino acid residues with a calculated molecular mass of 73,044 da. the active site-motifs sxxk, sxn, and ktg are present at the positions 393, 452, and 590, r ... | 2002 | 11866118 |
| enumeration of total bacteria and bacteria with genes for proteolytic activity in pure cultures and in environmental samples by quantitative pcr mediated amplification. | real-time quantitative pcr assays were developed for the absolute quantification of different groups of bacteria in pure cultures and in environmental samples. 16s rrna genes were used as markers for eubacteria, and genes for extracellular peptidases were used as markers for potentially proteolytic bacteria. for the designed 16s rdna taqman assay, specificity of the designed primer-probe combination for eubacteria, a high amplification efficiency over a wide range of starting copy numbers and a ... | 2002 | 11869788 |
| bacillus cereus cellulitis from contaminated heroin. | concern exists over recent unexplained deaths among intravenous drug users. this report describes a patient with crepitant cellulitis who was admitted complaining of severe pain in the right forearm. ultrasonography demonstrated gas in the tissues and he was referred for early surgical debridement of the arm. he was treated with intravenous benzyl penicillin, gentamicin and metronidazole and made a full recovery. aspirate samples grew bacillus cereus, morphologically similar to the isolate obtai ... | 2002 | 11871624 |
| acid stress in the food pathogen bacillus cereus. | the pathogen bacillus cereus, which is associated with a number of foods including dairy products, was studied for its response to acid stress during the exponential phase. | 2002 | 11872115 |
| genetic differentiation between sympatric populations of bacillus cereus and bacillus thuringiensis. | little is known about genetic exchanges in natural populations of bacteria of the spore-forming bacillus cereus group, because no population genetics studies have been performed with local sympatric populations. we isolated strains of bacillus thuringiensis and b. cereus from small samples of soil collected at the same time from two separate geographical sites, one within the forest and the other at the edge of the forest. a total of 100 b. cereus and 98 b. thuringiensis strains were isolated an ... | 2002 | 11872495 |
| inhibition of bacterial growth, enterotoxin production, and spore outgrowth in strains of bacillus cereus by bacteriocin as-48. | bacteriocin as-48 showed high bactericidal activity for mesophilic and psychrotrophic strains of bacillus cereus over a broad ph range. as-48 inhibition of the enterotoxin-producing strain lwl1 was enhanced by sodium nitrite, sodium lactate, and sodium chloride. the latter also enhanced as-48 activity against strain cect 131. bacterial growth and enterotoxin production by strain lwl1 were completely inhibited at bacteriocin concentrations of 7.5 microg/ml. at subinhibitory bacteriocin concentrat ... | 2002 | 11872505 |
| modified blasticidin s resistance gene (bsrm) as a selectable marker for construction of retroviral vectors. | retroviral vectors are commonly used in ex vivo gene therapy protocols. the structure of vectors basically consists of one gene of interest and a selectable marker gene. fast selection without damaging cells is a critical step for ex vivo gene therapy protocols. blasticidin s deaminase isolated from bacillus cereus has a neutralizing action on the highly toxic antibiotic blasticidin s (bs). a commercially available gene coding for blasticidin s deaminase (bsr) when used to construct retroviral v ... | 2002 | 11879712 |
| production of bacillus cereus emetic toxin (cereulide) in various foods. | to determine the role of bacillus cereus as a potential pathogen in food poisoning, the production of an emetic toxin (cereulide) by b. cereus was quantified in various food sources. the amount of emetic toxin in 13 of 14 food samples implicated in vomiting-type food poisoning cases ranged from 0.01 to 1.28 microg/g. a vomiting-type strain, b. cereus nc7401, was inoculated into various foods and incubated for 24 h at 20, 30, and 35 degrees c. in boiled rice, b. cereus rapidly increased to 10(7)- ... | 2002 | 11883672 |
| selective enrichment and characterization of a phosphorus-removing bacterial consortium from activated sludge. | under alternating aerobic/anaerobic conditions and without additional carbon sources, a bacterial consortium consisting initially of 18 bacterial strains was obtained in a sequence batch reactor. the phosphorus removal capability could only be maintained using sterile filtrate of activated sludge as medium. the addition of calcium and magnesium salts, as well as vitamins and trace elements, to autoclaved sterile filtrate of activated sludge was not sufficient to achieve stable phosphorus removal ... | 2002 | 11831470 |
| selection of bacteriocin producer strains of lactic acid bacteria from a dairy environment. | two strains showing bacteriocin production were selected from a total of 206 lactic acid bacteria isolated from samples of milk, milk serum, whey and homemade cheeses in southern cordoba, argentina. this property was detected by means of well diffusion assays. the strains were identified as enterococcus hirae and enterococcus durans. the protein nature of those substances was proved by showing their sensitivity to type iv and xxv proteases, papaine, trypsin, pepsin and k proteinase. the bacterio ... | 2002 | 11837389 |
| bacteriocin-producing lactobacillus sake as starter culture in dry sausages. | one hundred and fifty-two strains of lactobacillus spp and micrococcus spp, isolated from dry sausages, were screened for inhibitory activity. two of the strains assayed of the genus lactobacillus showed bactericidal activity. they were able to inhibit listeria monocytogenes, listeria seeligeri, listeria innocua, lactobacillus alimentarius and lactobacillus bavaricus. the strains of escherichia coli, salmonella bradford and salmonella newlands, staphylococcus aureus, bacillus cereus, pseudomonas ... | 2002 | 11837390 |
| fish chromatophores as cytosensors in a microscale device: detection of environmental toxins and bacterial pathogens. | fish chromatophores from betta splendens are used as the cytosensor element in the development of a portable microscale device capable of detecting certain environmental toxins and bacterial pathogens by monitoring changes in pigment granule distribution. the adaptation of chromatophores to a microscale environment has required the development of enabling technologies to produce miniaturized culture chambers, to integrate microfluidics for sample delivery, to miniaturize image capture, and to de ... | 2002 | 11841070 |
| sphingomyelinase cleavage of sphingomyelin in pure and mixed lipid membranes. influence of the physical state of the sphingolipid. | sphingomyelin hydrolysis by sphingomyelinase is essential in regulating membrane levels of ceramide, a well-known metabolic signal. since natural sphingomyelins have a gel-to-fluid transition temperature in the range of the physiological temperatures of mammals and birds, it is important to understand the influence of the physical state of the lipid on the enzyme activity. with that aim, large unilamellar vesicles consisting of pure egg sphingomyelin (gel-to-fluid crystalline transition temperat ... | 2002 | 11841822 |
| on calculating sterility in thermal preservation methods: application of the weibull frequency distribution model. | a simple and parsimonious model which originated from the weibull frequency distribution was proposed to describe nonlinear survival curves of spores. this model was suitable for downward concavity curves (bacillus cereus and bacillus pumilus), as well as for upward concavity curves (clostridium botulinum). it was shown that traditional f values calculated from this new model were no longer additive, to such an extent that a heat treatment should be better characterized by the obtained decimal r ... | 2002 | 11843401 |
| high-pressure inactivation of bacillus cereus spores in the presence of argon. | we investigated the high-pressure inactivation of bacillus cereus spores in water containing argon. at the pressure of 600 mpa, addition of argon accelerated the inactivation of spores at 20 degrees c, but had no effect on the inactivation at 40 degrees c. the influence of added argon on inactivation of the spores was marked under conditions with a strong 'water ordering' effect. the pressure resistance of b. cereus spores was thus shown to be affected by 'water ordering'. | 2002 | 11845823 |
| interaction of the rho-adp-ribosylating c3 exoenzyme with rala. | rhoa, -b, and -c are adp-ribosylated and biologically inactivated by clostridium botulinum c3 exoenzyme and related c3-like transferases. we report that rala gtpase, which is not adp-ribosylated by c3, inhibits adp-ribosylation of rhoa by c3 from c. botulinum (c3bot), clostridium limosum (c3lim), and bacillus cereus (c3cer) but not from staphylococcus aureus (c3stau) in human platelet membranes and rat brain lysate. inhibition by rala occurs with the gdp- and guanosine 5'-3-o-(thio)triphosphate- ... | 2002 | 11847234 |
| functional control of the binuclear metal site in the metallo-beta-lactamase-like fold by subtle amino acid replacements. | at present there are three protein families that share a common structural domain, the alphabeta/betaalpha fold of class b beta-lactamases: zinc beta-lactamases, glyoxalases ii, and a-type flavoproteins. a detailed inspection of their superimposed structures was undertaken and showed that although these proteins contain binuclear metal sites in spatially equivalent positions, there are some subtle differences within the first ligand sphere that determine a distinct composition of metals. althoug ... | 2002 | 11847294 |
| effect of nitrogen gas packaging on the quality and microbial growth of fresh-cut vegetables under low temperatures. | nitrogen (n2) gas packaging for fresh-cut vegetables (lettuce and cabbage) has been examined as a means of modified atmosphere packaging (map) for extending the shelf life of cut vegetables. gas composition in enclosed packages that contained cut vegetables and were filled with 100% n2 had an oxygen (o2) concentration of 1.2 to 5.0% and a carbon dioxide (co2) concentration of 0.5 to 3.5% after 5 days of storage. an atmosphere of low concentrations of o2 and high co2 conditions occurred naturally ... | 2002 | 11848563 |
| the influence of nisin on the thermal resistance of bacillus cereus. | decimal reduction times (d-values) at cooking and autoclaving temperatures (80 to 120 degrees c) of spores of bacillus cereus atcc 1479-8 in rice and milk (13% wt/vol) supplemented with nisin (25 microg/ml) were evaluated. the mean d-values at 97.8 degrees c in cooked white rice, phosphate buffer (ph 7.0), and rice water (ph 6.7) were 3.62, 1,99, and 1.34 min, respectively. from 80 to 100 degrees c, the mean reduction in d-values due to the addition of nisin to milk was 40%. the d-value at 110 d ... | 2002 | 11848577 |
| the effect of nisin on growth kinetics from activated bacillus cereus spores in cooked rice and in milk. | the growth kinetics of germinated cells from activated spores of bacillus cereus in cooked white rice and in milk were evaluated at different temperatures for control samples and for samples with 25 microg of nisin per ml added. nisin was applied in the form of nisaplin (10(6) iu/g), which contained 25,000 microg of nisin per g. the length of the lag phase for cooked white rice controls was 120 h at 10 degrees c, 8 h at 25 degrees c, and 2.5 h at 33 degrees c. the generation times for cooked ric ... | 2002 | 11848578 |
| adaptation of a neutrophilic dairy-associated bacillus cereus isolate to alkaline ph. | this study identified and studied the response of five bacillus strains, isolated from alkaline cleaning in place (cip) solutions, to alkaline conditions. | 2002 | 11849331 |
| differential efficacy of a chlorine dioxide-containing sanitizer against single species and binary biofilms of a dairy-associated bacillus cereus and a pseudomonas fluorescens isolate. | daily exposure to 100 p.p.m. chlorine dioxide of single species and binary biofilms of dairy-associated bacillus cereus dl5 and pseudomonas fluorescens m2, attached to stainless steel surfaces in a laboratory flow system, was studied. | 2002 | 11849365 |
| the antibacterial activity of triclosan-impregnated storage boxes against staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, bacillus cereus and shewanella putrefaciens in conditions simulating domestic use. | antimicrobial resistance has increased over the past decade causing concern for public health. domestic antimicrobial products containing triclosan (2,4,4'-trichloro-2'-hydroxydiphenylether), a broad-spectrum antibacterial agent, were introduced in 1997 and have become popular among consumers. cross-resistance to other antibacterial agents has been suggested as a possible consequence of their widespread use. triclosan-impregnated plastic storage boxes were tested for activity against staphylococ ... | 2002 | 11751771 |
| molecular cloning and expression of mn(2+)-dependent sphingomyelinase/hemolysin of an aquatic bacterium, pseudomonas sp. strain tk4. | we report here the molecular cloning and expression of a hemolytic sphingomyelinase from an aquatic bacterium, pseudomonas sp. strain tk4. the sphingomyelinase gene was found to consist of 1,548 nucleotides encoding 516 amino acid residues. the recombinant 57.7-kda enzyme hydrolyzed sphingomyelin but not phosphatidylcholine, phosphatidylserine, phosphatidylglycerol, phosphatidic acid, or phosphatidylethanolamine, indicating that the enzyme is a sphingomyelin-specific sphingomyelinase c. the hydr ... | 2002 | 11751833 |
| c3stau, a new member of the family of c3-like adp-ribosyltransferases. | c3-like adp-ribosyltransferases, which are produced by clostridium botulinum, clostridium limosum, bacillus cereus and staphylococcus aureus, are exoenzymes lacking a translocation unit. these enzymes specifically inactivate rho gtpases in host target cells. recently, a novel c3-like transferase from s. aureus with new properties was identified, raising questions regarding its function. as rho gtpases are master regulators of several eukaryotic signal processes and s. aureus can invade eukaryoti ... | 2002 | 11755073 |
| exploring the role and the binding affinity of a second zinc equivalent in b. cereus metallo-beta-lactamase. | metallo-beta-lactamases are a newly characterized family of zinc enzymes present in several pathogenic strains that represent an emerging clinical threat. enzymes from different organisms exhibit an outstanding functional diversity, particularly in the metal ion requirements for activity. we have investigated the effect of the second zinc(ii) equivalent in the enzyme betalii from bacillus cereus, naturally active in the mono-zinc(ii) form. the enzyme is reversibly inactivated at low ph, due to d ... | 2002 | 11827530 |
| recent developments in carbapenems. | carbapenems are beta-lactam antibiotics characterised by the presence of a beta-lactam ring with a carbon instead of sulfone in the 4-position of the thyazolidinic moiety. the first carbapenem to be utilised in therapy was imipenem, the n-formimidoyl derivative of thienamycin. imipenem is coadministered with cilastatin, an inhibitor of human renal dehydropeptidase i, as imipenem is hydrolysed by this enzyme. meropenem was the first carbapenem with a 1-beta-methyl group and 2-thio pyrrolidinyl mo ... | 2002 | 11922861 |
| catalytic mechanism of beta-amylase from bacillus cereus var. mycoides: chemical rescue of hydrolytic activity for a catalytic site mutant (glu367-->ala) by azide. | the hydrolytic activity of beta-amylase from bacillus cereus var. mycoides was lost on replacement of either of the catalytic residues (glu172 or glu367) with an alanyl residue. when maltopentaose and 2 m azide existed together mutant, e367a cleaved the glucosidic linkage of maltopentaose and produced maltose at ph 7.0 and 25 degrees c, but the other mutants (e172a and double mutant e172a/e367a) did not. this indicates that azide acts as a general base instead of e367 and glu172 acting as genera ... | 2002 | 11926997 |
| detection of bacillus cereus in foods by colony hybridization using pcr-generated probe and characterization of isolates for toxins by pcr. | isolates of bacillus cereus from traditional indian foods were detected by colony hybridization using the pcr-generated phospholipase (pl-1) probe. in all, 29 isolates picked up by the probe were confirmed as b. cereus by conventional cultural and biochemical characteristics. all the isolates reacted positively in pcr with phospholipase (pl-1) primers. among the native isolates, 11 of them showed the discontinuous pattern of haemolysin bl activity in gel diffusion assay. though 14 isolates react ... | 2002 | 11929167 |
| characterization of the genetic locus responsible for the production of abp-118, a novel bacteriocin produced by the probiotic bacterium lactobacillus salivarius subsp. salivarius ucc118. | abp-118, a small heat-stable bacteriocin produced by lactobacillus salivarius subsp. salivarius ucc118, a strain isolated from the ileal-caecal region of the human gastrointestinal tract, was purified to homogeneity. using reverse genetics, a dna fragment specifying part of abp-118 was identified on a 10769 bp chromosomal region. analysis of this region revealed that abp-118 was a class iib two-peptide bacteriocin composed of abp118alpha, which exhibited the antimicrobial activity, and abp118bet ... | 2002 | 11932444 |
| identification and characterization of toxigenic bacillus cereus isolates responsible for two food-poisoning outbreaks. | the epidemiology of bacillus cereus strains responsible for food poisoning is scantly known, mostly because the genotypic and toxigenic properties of the b. cereus strains isolated during food-poisoning outbreaks have been never catalogued. the occurrence of two simultaneous food-poisoning outbreaks gave us the opportunity to wonder whether (i) the identity of individual strains isolated from clinical, environmental, and food samples could be established by random amplified polymorphic dna (rapd ... | 2002 | 11934506 |
| quantitative analysis of cereulide, the emetic toxin of bacillus cereus, produced under various conditions. | this paper describes a quantitative and sensitive chemical assay for cereulide, the heat-stable emetic toxin produced by bacillus cereus. the methods previously available for measuring cereulide are bioassays that give a toxicity titer, but not an accurate concentration. the dose of cereulide causing illness in humans is therefore not known, and thus safety limits for cereulide cannot be indicated. we developed a quantitative and sensitive chemical assay for cereulide based on high-performance l ... | 2002 | 11976124 |
| the phenolic hydroxyl group of carvacrol is essential for action against the food-borne pathogen bacillus cereus. | the natural antimicrobial compound carvacrol shows a high preference for hydrophobic phases. the partition coefficients of carvacrol in both octanol-water and liposome-buffer phases were determined (3.64 and 3.26, respectively). addition of carvacrol to a liposomal suspension resulted in an expansion of the liposomal membrane. maximum expansion was observed after the addition of 0.50 micromol of carvacrol/mg of l-alpha-phosphatidylethanolamine. cymene, a biological precursor of carvacrol which l ... | 2002 | 11916669 |
| structural characterization of lipopeptide biomarkers isolated from bacillus globigii. | spectra obtained using matrix-assisted laser desorption/ionization (maldi) mass spectrometry of bacillus globigii (bacillus subtilis niger) spores, vegetative cells and the culture supernatant show a cluster of biomarkers centered at a molecular mass of 1478 da. three biomarkers were isolated from the cell-free culture supernatant by solid-phase extraction and reversed-phase high-performance liquid chromatography, and characterized using various kinds of mass spectrometry. a fourier transform ma ... | 2002 | 11921366 |
| microbiological quality and safety of ready-to-eat cooked foods from a centralized school kitchen in argentina. | the purpose of this study was to evaluate the microbiological and sensory quality as well as the safety of ready-to-eat (rte) cooked foods prepared in and distributed from a centralized kitchen to schools in argentina. a total of 101 cooked food samples delivered as hot rte cooked foods (group a) and as rte cooked foods at room temperature (group b) and 140 surface swab environment samples were collected from february to november 1999. petrifilm plates were used for aerobic (pac), coliform (pcc) ... | 2002 | 11952212 |
| isolation and characterization of bacillus cereus-like bacteria from faecal samples from greenhouse workers who are using bacillus thuringiensis-based insecticides. | since the discovery of the insecticidal activity of bacillus thuringiensis at the beginning of the twentieth century, this bacterium has been used increasingly against various insect pests. in spite of the extensive use of b. thuringiensis, only sporadic clinical case reports have been published. in recent years, the close relationship between b. thuringiensis and the human pathogen bacillus cereus has been confirmed. in practice, only the insecticidal activity of b. thuringiensis distinguishes ... | 2002 | 11954987 |
| design, synthesis and bioactivity evaluation of tribactam beta lactamase inhibitors. | known carbapenem compounds with inhibitory effect towards beta-lactamase enzymes are formed from bicyclical beta lactam structural scaffolds. on the basis of results from theoretical computational methods and molecular modelling we have designed and developed a synthetic route towards novel, biologically active tricyclic derivatives of carbapenems. | 2002 | 11959006 |
| toxicity testing and chemical analyses of recycled fibre-based paper for food contact. | food-contact materials, including paper, have to comply with a basic set of criteria concerning safety. this means that paper for food contact should not give rise to migration of components, which can endanger human health. the objectives of this pilot study were, first, to compare paper of different qualities as food-contact materials and to perform a preliminary evaluation of their suitability, from a safety point of view, and, second, to evaluate the use of different in vitro toxicity tests ... | 2002 | 11962701 |
| sporulation and delta-endotoxin synthesis by bacillus thuringiensis. | bacillus thuringiensis is distinguished from the very closely related bacillus cereus and bacillus anthracis by the presence of several plasmid-encoded delta-endotoxin genes. these delta-endotoxins, synthesized as protoxins, are produced in large quantities during sporulation and are packaged into intracellular inclusions. ingestion of the inclusions by insect larvae leads to protoxin solubilization and conversion to toxins each specific for one of several orders of insects. the toxins form cati ... | 2002 | 11964120 |
| mutational analysis of the two zinc-binding sites of the bacillus cereus 569/h/9 metallo-beta-lactamase. | the metallo-beta-lactamase bcii from bacillus cereus 569/h/9 possesses a binuclear zinc centre. the mono-zinc form of the enzyme displays an appreciably high activity, although full efficiency is observed for the di-zinc enzyme. in an attempt to assign the involvement of the different zinc ligands in the catalytic properties of bcii, individual substitutions of selected amino acids were generated. with the exception of his(116)-->ser (h116s), c221a and c221s, the mono- and di-zinc forms of all t ... | 2002 | 11964169 |
| substrate-activated zinc binding of metallo-beta -lactamases: physiological importance of mononuclear enzymes. | we have investigated the influence of substrate binding on the zinc ion affinity of representatives from the three metallo-beta-lactamase subclasses, b1 (bcii from bacillus cereus and blab from chryseobacterium meningosepticum), b2 (cpha from aeromonas hydrophila), and b3 (l1 from stenotrophomonas maltophilia). by competition experiments with metal-free apoenzymes and chromophoric zinc chelators or edta, we determined the dissociation constants in the absence and presence of substrates. for the ... | 2002 | 11967267 |
| discrimination of bacillus cereus and bacillus thuringiensis with 16s rrna and gyrb gene based pcr primers and sequencing of their annealing sites. | to evaluate the possibility for discrimination of bacillus cereus and b. thuringiensis using 16s rrna and gyrb gene based pcr methods, and to obtain the sequences of the primer annealing sites so that the pcr results may be explained. | 2002 | 11972696 |
| phospholipase c-dependent hydrolysis of phosphatidylcholine hydroperoxides to diacylglycerol hydroperoxides and its reduction by phospholipid hydroperoxide glutathione peroxidase. | we have shown that 1,2-diacylglycerol hydroperoxides activate protein kinase c (pkc) as efficiently as does phorbol ester [takekoshi s, kambayashi y, nagata h, takagi t, yamamoto y, watanabe k. activation of protein kinase c by oxidized diacylglycerol. biochem biophys res commun 1995; 217: 654-660]. 1,2-diacylglycerol hydroperoxides also stimulate human neutrophils to release superoxide whereas their hydroxides do not [yamamoto y, kambayashi y, ito t, watanabe k, nakano m. 1,2-diacylglycerol hyd ... | 2002 | 11981452 |
| microbial transformation of danazol. | danazol (17beta-hydroxy-17alpha-pregna-2,4-dien-20-yno-[2,3-d] isoxazole) (1) on fermentation with fusarium lini, aspergillus niger, and cephalosporium aphidicola yielded 17beta-hydroxy-2-(hydroxymethyl)-17-alpha-pregn-4-en-20-yn-3-one (2) and 17beta-hydroxy-2-(hydroxymethyl)-17 alpha-pregna-1,4-dien-20-yn-3-one (3), while the fermentation of 1 with bacillus cerus yielded compound 2 only. | 2002 | 11990425 |
| factors affecting the adsorption of buchnericin lb, a bacteriocin produced by lactobacillus [correction of lactocobacillus] buchneri. | buchnericin-lb adsorbs to gram-positive but not to gram-negative bacteria. the tested gram-positive bacteria were species of lactobacillus, pediococcus, leuconostoc, enterococcus, lactococcus, listeria, bacillus, staphylococcus; gram-negative bacteria belonged to the genera salmonella, escherichia, yersinia and pseudomonas. buchnericin-lb adsorption depended on ph but not on time and temperature. also some anions of salts and lipoteichoic acid reduced or inhibited its adsorption. treatment of ce ... | 2002 | 12002398 |
| variation in rrna operon number as revealed by ribotyping of bacillus anthracis strains. | ribotyping of various bacillus strains with one restriction enzyme (acci) revealed significant similarity between bacillus anthracis strains, bacillus thuringiensis and bacillus cereus strains, which are all members of the bacillus cereus group. a further ribotyping study of 10 virulent and 8 attenuated b. anthracis strains, using 4 endonucleases and both 23s and 16s probes independently, was performed. the discrimination index d of hunter and gaston showed that the best combination for future l ... | 2002 | 12002563 |
| essential oil constituents and in vitro antimicrobial activity of decalepis hamiltonii roots against foodborne pathogens. | hydrodistillation of decalepis hamiltonii roots yielded an essential oil (0.33% v/w) that contained 2-hydroxy-4-methoxybenzaldehyde (37.45%), 2-hydroxybenzaldehyde (31.01%), 4-o-methylresorcylaldehyde (9.12%), benzyl alcohol (3.16%), and alpha-atlantone (2.06%) as major constituents, with aromatic aldehydes constituting the main fraction of this root's essential oil. the oil was tested for its antimicrobial activity against foodborne pathogens responsible for food spoilage and human pathologies ... | 2002 | 12009977 |
| cysteine-less glycosylphosphatidylinositol-specific phospholipase c is inhibited competitively by a thiol reagent: evidence for glyco-mimicry by p-chloromercuriphenylsulphonate. | glycosylphosphatidylinositol (gpi)-specific phospholipases are highly valuable for studying the structure and function of gpis. gpi-specific phospholipase c (gpi-plc) from trypanosoma brucei and phosphatidylinositol-specific phospholipase c (pi-plc) from bacillus cereus are the most widely studied of this class of phospholipases c. inhibition of protein activity by thiol reagents is indicative of the participation of cysteine residues in biochemical events. the thiol reagent p-chloromercuripheny ... | 2002 | 12010122 |
| phages and other mobile virulence elements in gram-positive pathogens. | 2002 | 12012871 | |
| genome structure and evolution of the bacillus cereus group. | 2002 | 12012872 |