Publications

monoclonal antibodies against the surface antigens of shigella flexneri serotype 1b and shigella dysenteriae serotype 1.	monoclonal antibodies against the surface antigens of shigella flexneri 1b and s. dysenteriae 1 were prepared. the specificities of the antibodies were evaluated by enzyme-linked immunosorbent assay (elisa), and quantitative agglutination using microtiter plate. monoclonal antibodies against s. flexneri 1b, designated sf2b2 and sf2g4, belonged to igg2a and igg1 subclass, respectively. the former was specific for s. flexneri 1b, whereas the latter was reactive not only to s. flexneri 1b, but also ...	1989	2687648
toxic megacolon and perforation caused by shigella.		1989	2688808
shigella colitis with radiological and endoscopic correlation: case report.	we report a patient with acute colitis secondary to shigella flexneri infection. radiologic and endoscopic studies were obtained during and after the patient's clinical recovery. hypotonic air contrast barium enemas were helpful in evaluating the severity and extent of the disease proximal to the segment viewed by flexible sigmoidoscopy. they showed pseudopolyposis, which has not been previously reported in the acute phase, and other mucosal abnormalities which persisted long after clinical reco ...	1989	2688842
nutritional anestrus in beef cows: concentrations of glucose and nonesterified fatty acids in plasma and insulin in serum.	twenty-two nonlactating hereford cows exhibiting normal estrous cycles were fed either maintenance (m) or restricted (r) diets until most of the r cows became anestrus; r cows then were fed 160% of the m diet until estrous cycles resumed. concentrations of progesterone, glucose, insulin and nonesterified fatty acids (nefa) were determined in weekly blood samples. blood also was collected frequently, before and after i.v. infusion of 300 ml of a 40% glucose solution, to evaluate responses in bloo ...	1989	2689418
biofilm model experiments in vitro with escherichia coli and shigella strains.	escherichia coli and shigella were cultured together in a glucose-free minimal medium completed only by hog gastric mucin. in mixed cultures inoculated simultaneously one member of the pair more or less overgrew the other. this organism remained predominant when superinfected with its strain pair after 1, 3, 5 or 7 days incubation. other signs of the quasi biofilm character of such cultures are the higher level of free polysaccharides and enhanced viscosity in the medium and higher streptomycin ...	1989	2690554
[field trials with an oral cell-free vaccine made from shigella flexneri].	the data on the study of the reactogenicity, safety and prophylactic potency of a new acellular vaccine prepared from s. flexneri 2a antigenic complexes are presented. according to the results of two epidemic experiments, the vaccine, introduced by oral administration, showed low reactogenicity, safety and sufficient prophylactic potency. the vaccine decreased morbidity rate in dysentery caused by s. flexneri 2a and ensured the protection of 74% (72-80%) of the vaccinees. the complete course of ...	1989	2692352
platelet-activating factor increases platelet-dependent glycoconjugate secretion from tracheal submucosal gland.	using isolated glands from feline trachea, we examined the effect of platelet-activating factor (paf) on radiolabeled glycoconjugate release and glandular contraction by measuring induced tension in the absence or presence of platelets. paf alone did not produce any significant glandular contraction nor any significant change in glycoconjugate release from isolated glands. in the presence of purified platelets containing no plasma, paf (10(-8) to 10(-5) m) produced significant glycoconjugate sec ...	1989	2692465
falciparum malaria--present day problems. an experience with 425 cases.	clinical details and present day problems encountered in 425 cases of falciparum malaria (pf) are reported. 10.11% had taken chloroquine prior to reporting to us. parasitic count done in 23.05% cases lacked correlation with severity of disease. pattern of fever varied markedly but 5.4% were afebrile throughout and presented only with bodyache and malaise. apyrexial spell was noted in 5.64%. 28.70% had typical facial looks of anaemia and sallow complexion. cerebral symptoms were noted in 3.05%. o ...	1989	2693436
the genetics of protein targeting in escherichia coli k12.	genetic analysis of protein targeting in escherichia coli has been facilitated by the use of lacz gene fusions. strains which contain a fusion between a gene specifying a noncytoplasmic protein and lacz exhibit novel phenotypes that can be exploited to obtain export-defective mutations. analysis of these mutations has demonstrated the importance of the signal sequence and provided some insight into the functions performed by this complex intragenic export signal. in addition, genes that specify ...	1989	2693457
[clinical trial of t-3262 (tosufloxacin tosilate) on salmonella enteritis, and fecal drug concentration and change in the fecal microflora in the acute diarrheal patients. japan research committee of t-3262, research group for acute infectious enteritis].	for the purpose of evaluation of clinical efficacy, safety and usefulness on salmonella enteritis, t-3262 (tosufloxacin tosilate), a newly developed pyridone-carboxylic acid derivative, was administered to a total of 103 patients and carriers. in addition, in vitro antibacterial activity of t-3262 was determined against the clinical isolates, and compared with those of nalidixic acid (na), pipemidic acid (ppa), enoxacin (enx), norfloxacin (nflx) and ofloxacin (oflx). and when t-3262 was administ ...	1989	2693543
[the role of the antilysozyme activity of shigella flexneri as a factor controlling the degree of its resistance to the lytic action of bacteriophages].	the level of the antilysozyme activity of s. flexneri in ensuring the high level of their phage resistance has been studied. the realization of the phage protective effect of antilysozyme activity has been noted to occur due to disturbances in the lysis of infected bacteria by phage-synthetized lysozyme-like enzyme. the direct relationship between the level of the lysozyme production of bacteriophages and their capacity for overcoming the antilysozyme protection of the host bacterium has been sh ...	1989	2694689
[interleukin-1-inducing activity of the polysaccharide-containing antigens of the cell wall in yersinia pestis].	the induction of the synthesis of interleukin-1 (il-1) in human monocytes under the influence of the endotoxic preparations (lps) and y. pestis basis somatic antigen has been experimentally studied. the results obtained in this study make it possible to come to the conclusion that the capacity of the endotoxin of y. pestis cell wall, consisting of lps of type r, for inducing the synthesis of il-1 in human monocytes is not different from the corresponding capacity of salmonella and shigella lps, ...	1989	2694696
[resistance of shigella to antibiotics].	antibiotic sensitivity of 104 shigella clinical strains and 104 escherichia coli strains isolated from patients with acute dysentery not treated with antibiotics in 1986-1987 was studied. it was shown that 100 per cent of the dysentery pathogens and colon bacilli were antibiotic resistant. strains resistant simultaneously to chloramphenicol, ampicillin, streptomycin, tetracycline, monomycin and kanamycin were the most frequent among the dysentery pathogens. colon bacilli and dysentery pathogens ...	1989	2694990
extra-intestinal manifestations of shigellosis.		1989	2695518
[molecular pathogenesis of bacillary dysentery caused by shigella flexneri].		1989	2695659
evidence for clathrin mobilization during directed phagocytosis of shigella flexneri by hep2 cells.	the enteroinvasive species shigella flexneri expresses a plasmid-mediated capacity to penetrate epithelial cells by directed phagocytosis involving actin polymerization. in the present work, hep2 cells were depleted of intracellular k+ in order to arrest receptor-mediated endocytosis and to evaluate the role of this endocytic pathway in the internalization of invasive microorganisms. such a treatment, which efficiently inhibited diphtheria toxin endocytosis and dissociated clathrin coats of the ...	1989	2695740
genotoxicity testing: phage t7 inactivation test of various furan and arenofuran derivatives.	the genotoxic activities of 28 furan and arenofuran derivatives were tested by the phage t7-inactivation test. the genotoxic activity of the compounds was characterized quantitatively. all the compounds studied have pronounced genotoxic activities in our system. empirical rules relating structure to genotoxic activity were found. data obtained with our system were compared with the results of other biological systems (salmonella assay, sos chromotest, cho/hgprt, gene amplification) in the case o ...	1989	2695762
characterization and autoprocessing of precursor and mature forms of human immunodeficiency virus type 1 (hiv 1) protease purified from escherichia coli.	a recombinant plasmid encompassing the human immunodeficiency virus type 1 (hiv 1) protease coding sequence and flanking regions (ala-13 to gly-185 of the pol open reading frame) has been expressed in two distinct strains of escherichia coli, ar58 and ar68. in the first strain, ar58, the primary translation product, a 25 kilodalton (kda) precursor protein, is short-lived and rapidly processes itself to the 11 kda mature protease in vivo. in the second strain, ar68, the 25 kda species is only par ...	1989	2695927
fulminating encephalopathy associated with shigella flexneri.		1989	2696551
[clinico-morphological criteria of diagnosis of dysentery and salmonella infections].	clinical symptoms and laboratory findings have been evaluated for 108 patients with intestinal infection associated with apparent colitic syndrome. differential diagnosis between dysentery and salmonellosis was not an easy task even though clinical signs of the diseases appeared different. dysentery ran with tenesmus, sigmoid pain and spasms. symptoms of gastritis, intoxication, dehydration, hepatomegaly predominated in salmonellosis. in obscure cases colonic biopsy can be helpful due to high se ...	1989	2696830
transferable trimethoprim resistance of shigellae encountered in vellore (south india).	shigella flexneri and sh. shigae which are the two most common shigellae encountered in vellore (south india) were found to exhibit resistance to trimethoprim and trimethoprim-sulphamethoxazole. eighty four per cent of this was high level resistance. transfer studies conducted with these strains indicated that this high level resistance is plasmid mediated.	1989	2697688
protein-losing enteropathy.		1989	2697925
[the etiological structure of shigellosis in the ussr in recent years].	the characterization of etiological structure of shigella infection in the whole of the ussr, in individual union republics and at a number of other administrative territories of the ussr in recent years is presented. s. flexneri has been shown to prevail at the territories with unsatisfactory water supply of the population, and s. sonnei prevails at the territories with good water supply. at the former territories s. dysenteriae and s. boydii retain their etiological importance, while at the la ...	1989	2698030
[the role of delayed hypersensitivity in the development of an experimental dysentery infection and vaccinal process].	the results of experiments, indicating the development of cell-mediated delayed hypersensitivity (dh) skin reaction, its dependence on the virulence of shigella flexneri 2a, the dose and the character of the antigen used for testing the reaction, are presented. one of the immunologically active components of s. flexneri virulent strain 2a no. 516 is a biologically active thermolabile factor detected in filtrate and supernatant fluid. these data suggest that the immunosuppressive action of the vi ...	1989	2698032
clinical role of granulocyte-macrophage colony-stimulating factor.	the activity of recombinant human granulocyte-macrophage colony-stimulating factor (gm-csf) for increasing leukocyte production and enhancing mature neutrophil function has been clearly demonstrated in phase i clinical trials in patients with a variety of hematologic and malignant diseases. the focus of current studies includes determination of optimal administration schedules and its use in combination with myelosuppressive chemotherapy, radiation therapy or antimicrobial agents. the utility of ...	1989	2698875
[prostaglandins and cyclic nucleotides in the gastrointestinal form of yersiniosis].	in spite of the resemblance of the clinical picture of gastrointestinal yersiniosis and acute dysentery, material differences underlie the pathogenesis of these diseases. yersiniosis is marked by the predominance of an increase in the content of pgf2 alpha, whereas acute dysentery by an increase in the content of pge, which may be accounted for by greater intensity of the allergic manifestations in yersiniosis patients as compared with dysentery. shigellosis runs its course in the presence of th ...	1989	2699095
[the importance of immunofluorescence and cytologic indices in the diagnosis and prognosis of the course of dysentery].		1989	2699099
effects of endothelin on the cardiovascular system and on smooth muscle preparations in different species.	(1) the actions of porcine endothelin (et), an endothelium-derived vasoconstrictor peptide, have been investigated in several in vitro smooth and cardiac muscle preparation as well as on the blood pressure of anaesthetized rats and rabbits. (2) in isolated visceral smooth muscles (guinea pig ileum, rat colon and uterus, rabbit jejunum) et caused a long-lasting contraction which persisted after repeated rinsing. et did not interfere with the spontaneous phasic activity of the rabbit jejunum or wi ...	1989	2699356
production and characterization of monoclonal antibodies with diagnostic potential against shigella flexneri.	highly specific monoclonal antibodies (mcabs), 1am3, 2am1 and 2bm2 were produced and characterized against sh. flexneri 1a, 2a and 2b respectively. iam3 is an igg3 (lambda) type antibody reactive against whole bacteria or the lipopolysaccharide (lps) only; 2am1 is an igg1 (kappa) type antibody which reacts with the polysaccharide component of lps; 2bm2 is an igg3 (lambda) type antibody which binds with both the polysaccharide and protein/peptide components of the lps. the results indicate that s ...	1989	2700207
current management of salivary gland tumors. part 2.	the authors base their treatment of salivary gland malignancies on the size of the primary and the histopathologic diagnosis. group 1 includes smaller tumors in the t1 and t2 classification with cell types that are associated with slow growth. a parotidectomy is usually sufficient therapy for tumors in this group. group 2 contains t1 and 2 tumors with more aggressive behavior. total parotidectomy is indicated here, with postoperative radiotherapy. t3 tumors and patients with nodal metastasis or ...	1989	2701414
[resonance stimulation of regional ventilation of the lungs by sound vibration in the prevention of acute respiratory insufficiency].		1989	2704716
abnormalities in the levels of extracellular and tissue amino acids in the brain of the seizure-susceptible rat.	basal and high potassium-stimulated release of endogenous amino acids was measured using brain dialysis in the hippocampus of urethane-anesthetized seizure-resistant (sr) and seizure-susceptible (ss) rats. moreover, the tissue level of amino acids was determined in the hippocampus, sensorimotor cortex, cerebellum and corpus striatum. the basal extracellular concentration of amino acids did not differ between sr and ss rats. however, aspartate release was higher, and taurine and phosphoethanolami ...	1989	2707250
preliminary notes on vulnerability of the retina to injury from peripheral laser exposure conditions.	if laser exposures to the periphery of the retina from low-energy lasers cause a hemorrhage obscuring the optical pathway to the fovea, resulting in loss of visual function, the risks for soldiers on the battlefield may be greater than previously expected. two off-axis experiments were undertaken to obtain an indication of the hazards involved in exposing the peripheral retina to visible laser irradiation. both off-axis exposures caused immediate vitreal hemorrhages in the periphery. after 80 mi ...	1989	2708055
differential expression of transforming growth factors alpha and beta in rat intestinal epithelial cells.	expression of transforming growth factor alpha (tgf alpha), and transforming growth factor beta (tgf beta) was assessed in isolated primary rat intestinal epithelial cells as well as a rat intestinal crypt cell-derived cell line (iec-6). a gradient in tgf beta was present, with high concentrations of a 2.5-kb transcript found in undifferentiated crypt cells and progressively lower amounts of the tgf beta transcript in increasingly differentiated villus cell populations. in contrast, the concentr ...	1989	2708531
topographic organization of the central projections of the spiral ganglion in cats.	the morphological organization of inputs from restricted sectors of the cat cochlear spiral ganglion into the cochlear nucleus was studied by making focal extracellular injections of horseradish peroxidase (hrp) into the spiral ganglion. injections resulted in golgi-like labeling of a small cluster of spiral ganglion cells and their peripheral and central axons. large injections involved most of the cells within rosenthal's canal in sectors of the spiral ganglion innervating greater than or equa ...	1989	2708585
tyrosine phosphorylation in the postnatal rat brain: a developmental, immunohistochemical study.	we have used antibodies against phosphotyrosine to probe 50-microns cerebellar sections from rats of various ages as well as sections of adult brainstem, cerebrum, and olfactory bulb to investigate the developmental appearance of this phosphorylation system as revealed by light microscopy. while the overall intensity of staining in the cerebellum was highest at 7 days, the pattern of staining in the adult is quite disparate from that seen in younger animals. from 10 to 21 days postnatal, stainin ...	1989	2708590
effect of surfactant-associated protein-a (sp-a) on the activity of lipid extract surfactant.	the properties of natural bovine surfactant and its lipid extract have been examined with a pulsating bubble surfactometer which assesses the ability of surfactant lipids to adsorb to the air/liquid interface and reduce the surface tension to near 0 dynes/cm during dynamic compression. studies conducted at 1 mg/ml phospholipid revealed that the surface activity (i.e., the ability to produce low surface tensions) of lipid extracts could be enhanced by incubating the sample at 37 degrees c for 120 ...	1989	2713385
effect of estradiol on the phosphorylation of rat liver ribosome proteins.	the effect of estradiol on phosphorylation with gamma-32p-atp of ribosome proteins from polyribosome fraction, isolated from female rat liver is found out. rats (180 g) used were: sham-operated, ovariectomized and treated after ovariectomy for 11 days with 20 micrograms 17 beta-estradiol-dipropionate per 100 g body weight. the estradiol deficiency after ovariectomy decreased phosphorylation, but the estrogenization of ovariectomized rats increases it twice. in vitro the hormone (1-2 mumol/l) [co ...	1989	2714224
early plaque colonization on human cementum.	we have described the morphology of developing plaque on cementum in an in vivo human model. slabs of cementum obtained from sound teeth, rendered anorganic with 5% sodium hypochlorite, were glued to orthodontic brackets and positioned on the upper canines, premolars and molars in 8 volunteers. the brackets were removed after 2, 4, 8, 24 h and processed for scanning electron microscopy (sem). within 2 h, a thin pellicle covered the cementum surface, with few micro-organisms detectable. at 4 and ...	1989	2715365
production of monoclonal antibodies toward bovine interferons-alpha suitable for immunopurification.	five murine hybridoma clones, producing monoclonal antibodies (mabs) to bovine interferon-alpha (boifn-alpha) were established. one of these, f12, secreted mabs giving high titers, in elisa tests, neutralizing both boifn-alpha, -alpha c, and boifn-alpha d activities, belonging to the mouse igg1 class, and having a binding affinity constant of 10(8) m-1. f12 mabs were used for immunoaffinity purification of boifn-alpha, and recombinant boifn-alpha c from escherichia coli extracts was purified to ...	1989	2715671
immunoblotting analysis of the reaction of wildebeest, sheep and cattle sera with the structural antigens of alcelaphine herpesvirus-1 (malignant catarrhal fever virus).	malignant catarrhal fever (mcf) is a disease of cattle and some other ruminants caused by alcelaphine herpesvirus-1 (ahv-1), a virus of wildebeest. the disease also occurs in the absence of wildebeest and is then thought to be caused by a viral agent harboured by the sheep. the structural proteins of ahv-1 have been used as antigens for the immunoblotting analysis of sera from wildebeest, sheep and cattle infected by either ahv-1 or the "sheep-associated" form of the disease. wildebeest sera sho ...	1989	2718352
[viral infection and obstructive cholangiography in children].	in the last years it has been suggested that idiopathic obstructive cholangiopathy is a pathologic process with evolutive studies: extrahepatic biliary atresia and neonatal hepatitis. the possibility of a viral agent as the inciting factor has been investigated. this report shows four male and one female with confirmed idiopathic obstructive cholangiopathy with different clinical picture. there was evidence of cytomegalovirus infection in two patients, rubella infection in one patient, hepatitis ...	1989	2719422
a light microscopic and autoradiographic study of non-irradiated and irradiated ocular wounds.	focal gamma irradiation was used to limit the intraocular extension of scar tissue which typically occurs after posterior perforating injury to the eye. standard posterior perforating injuries were created in the right eye of forty-eight rabbits, half of which had the site of perforation focally irradiated using a cobalt 60 ophthalmic plaque. non-irradiated wounds healed with profuse formation of highly cellular and vascularised granulation tissue which invaded the vitreous to form contractile v ...	1989	2721224
a light microscopic and autoradiographic study of non-irradiated and irradiated ocular wounds.	focal gamma irradiation was used to limit the intraocular extension of scar tissue which typically occurs after posterior perforating injury to the eye. standard posterior perforating injuries were created in the right eye of forty-eight rabbits, half of which had the site of perforation focally irradiated using a cobalt 60 ophthalmic plaque. non-irradiated wounds healed with profuse formation of highly cellular and vascularised granulation tissue which invaded the vitreous to form contractile v ...	1989	2721224
the effects of ultrasonically induced hyperthermia on experimental tumors in the rabbit eye.	greene's melanomas, implanted in the anterior chamber of 31 eyes of 19 dutch rabbits, were treated with hyperthermia induced by high intensity focused ultrasound (hifu). the percentage necrosis 24 hr after treatment (n) as a function of steady-state intratumor temperature (t) could be described with n(t) = -181.5 + 4.7t (p less than 0.05). the most frequent side effect was a local hemorrhagic keratitis. the acoustic absorption coefficient and thermal conductivity of the tumor tissue were 0.08 cm ...	1989	2722440
assessment of the conduction of the cardiac impulse by a new epicardiac surface and stimulation technique (sst-ecg) in langendorff perfused mammalian hearts.	the assessment of the effects on the conduction of the cardiac impulse and of refractoriness simultaneously from multiple cardiac structures is important to evaluate the mode of action of new compounds, as well as to investigate undesired cardiac side effects. the measurements of intracardiac electrical activities of special structures have required catheter methods or averaging techniques to produce clear deflections of low-level potential wave forms. we have developed an epicardial surface ele ...	1989	2724989
neuropeptide y afferents have synaptic interactions with histaminergic (histidine decarboxylase-immunoreactive) neurons in the rat brain.	the synaptic interaction was identified between histaminergic cells and neuropeptide y (npy)-immunoreactive axon terminals in the caudal magnocellular nucleus, by means of the immunoelectron microscopic mirror method. this observation suggests that npy afferents exert monosynaptic influences upon the functioning of the central histaminergic neuronal system.	1989	2725952
human basic fibroblast growth factor gene encodes four polypeptides: three initiate translation from non-aug codons.	human basic fibroblast growth factor (bfgf) is an angiogenic polypeptide mitogen present in a wide variety of mesoderm- and neuroectoderm-derived tissues. bfgf cdna and genomic clones predict a 17.8-kda (155-amino acid) gene product based on the presence of a single putative translational initiator atg codon. however, a bfgf protein isolated from human placenta contains two additional amino acids nh2-terminal to the predicted initiator methionine. we report here that the human cell line sk-hep-1 ...	1989	2726761
large phosphate shifts with treatment for hyperglycemia.	although hypophosphatemia is known to commonly accompany therapy for hyperglycemia, it is not generally appreciated that severe life-threatening depletion of phosphate may occur. i followed up two patients who had precipitous drops during intravenous insulin therapy for diabetic ketoacidosis and hyperosmolar nonketotic state. the patient with diabetic ketoacidosis had a phosphate level nadir that equaled the lowest recorded phosphate level in a living human (0.03 mmol/l). because (1) serum phosp ...	1989	2730261
hypoxic pulmonary vasoconstrictor response with asymmetric oleic acid injury in the dog.	the hypoxic pulmonary vasoconstrictive (hpv) response is characterized by two components: intrapulmonary blood flow redistribution and pulmonary artery pressure (pap) augmentation. whether both remain intact after oleic acid lung injury has not been assessed. in an asymmetric model of right lung oleic acid edema, we examined the relative magnitude of each component on the hpv response to right lung inspired hypoxia. before oleic acid administration, right lung hypoxia decreased perfusion to the ...	1989	2736926
the practice of euthanasia in the netherlands.		1989	2737857
biological properties of streptonigrin derivatives. iii. in vitro and in vivo antiviral and antitumor activities.	antitumor antibiotic streptonigrin (stn-cooh) is a potent inhibitor of avian myeloblastosis virus (amv) and human immunodeficiency virus reverse transcriptases. the carboxyl group at 2'-position of stn-cooh was modified to give esters, hydrazide, amides and amino acid derivatives for biological studies. against amv reverse transcriptase, the hydrazide, amides and amino acid derivatives showed inhibitory activity, which compared favorably to that of stn-cooh, with the id50 values ranging 2-8 micr ...	1989	2737955
leucine-zipper motif update.		1989	2739731
endogenous serum albumin content in brain after short-lasting epileptic seizures.	epileptic seizures can transiently alter the blood-brain barrier. we have determined the content of extravasated endogenous serum albumin in the brain and its change with time after bicuculline (0.3 mg/kg) induced epileptic seizures of a few minutes' duration in conscious rats. the brains were perfused with saline in situ 5 min, 2 h, 24 h, 3 or 7 days after the injection of bicuculline. the content of endogenous serum albumin in the cerebral cortex, diencephalon, mesencephalon, pons and cerebell ...	1989	2743155
glycaemic effects of traditional european plant treatments for diabetes. studies in normal and streptozotocin diabetic mice.	twelve plants used for the traditional treatment of diabetes mellitus in northern europe were studied using normal and streptozotocin diabetic mice to evaluate effects on glucose homeostasis. the plants were administered in the diet (6.25% by weight) and/or as decoctions or infusions in place of drinking water, to coincide with the traditional method of preparation. treatment for 28 days with preparations of burdock (arctium lappa), cashew (anacardium occidentale), dandelion (taraxacum officinal ...	1989	2743711
[surgical proposals concerning omental mesh graft. experimental studies].	in general surgery, the advantages deriving from the utilisation of omental graft are attributable to the ease with which it takes, its regenerating potential and the modulator action on tissue repair. personal experience regarding the peritoneization and covering of surgical liver surfaces is reported and the use in various sectors of surgery of omental mesh graft is recommended.	1989	2747948
heterogeneity of anti-u demonstrable by the use of papain-treated red cells.	when red cells (rbcs) are treated with papain, one form of the u antigen, which we have named ups (u papain-sensitive), is almost completely removed or denatured. a second form, upr (u papain-resistant), remains unaltered on the treated rbcs. tests on 42 examples of anti-u showed that two contained only anti-ups, 19 contained only -upr, and 21 contained separable -ups and -upr. in those sera containing both antibodies, anti-upr was always the stronger of the two. these findings suggest 1) that u ...	1989	2749873
morphology of the bovine sertoli cell during the spermatogenetic cycle.	the morphology of the bovine sertoli cell was studied during 6 different phases of the spermatogenetic cycle. tubular dimensions do not vary significantly during the phases. sertoli cells occupy 27.0% (phase 4) to 38.4% (phase 8) of the tubular epithelium. sertoli cells of phase 1 are approximately 20% larger than during the other phases. 30-35% of sertoli cell volume consists of organelles. mitochondrial (about 5.0%) and nuclear (about 5.7%) volume densities remain remarkably stable during the ...	1989	2752416
enhancement of radiation-induced dna double-strand breaks and micronuclei in human colon carcinoma cells by n-methylformamide.	the differentiation-inducing agent n-methylformamide (nmf) enhances the sensitivity of some cell lines to ionizing radiation. to elucidate the mechanism of nmf-mediated radiosensitization, we examined the effects of this agent on gamma-ray-induced dna double-strand breaks and micronuclei in two cell lines, clone a (human colon carcinoma) and hca-1 (murine hepatocarcinoma). both cell lines form a better differentiated phenotype upon exposure to nmf, yet only clone a is radiosensitized. the neutra ...	1989	2756107
perioral anesthesia disrupts maternal behavior during early lactation in long-evans rats.	the role of perioral somatosensory stimulation from pups in maternal behavior was investigated in lactating long-evans rats (rattus norvegicus) during early lactation by local anesthesia of the mystacial pads with lidocaine, which blocks conduction in the infraorbital nerve. it was concluded that (1) acute perioral anesthesia markedly inhibited pup retrieval when the subjects had no formal pretreatment retrieval experience. further, these dams displayed little tolerance to the lidocaine treatmen ...	1989	2757582
biological activity in the repopulating rat spermatocyte after the withdrawal of gossypol treatment: ii. the activity for the synthesis of cellular and nuclear basic proteins.	the synthesis of cellular and nuclear basic proteins in the first generation of the repopulated pachytene spermatocyte (rps) and the control pachytene spermatocyte (cps) was studied by the incorporation of 3h-arginine into proteins. after pretreatment with gossypol for various lengths of time, the synthetic activity for cellular protein in the rps was slightly inhibited. in comparison to cps, on the other hand, the inhibition ranged from 12% to 26% (for gossypol pretreatment from 3 to 12 weeks, ...	1989	2758844
relationship between concentration and anticonvulsant effect of phenytoin against electroshock-induced seizures in rats: comparison of sampling sites for concentration determinations.	the purpose of this investigation was to determine the optimum sampling site for phenytoin concentration measurements in the context of pharmacodynamic studies of the anticonvulsant effect of phenytoin. determination of drug concentrations in the serum, serum water, brain, and cerebrospinal fluid (csf) of rats as a function of time after iv injection of a 6-mg/kg dose revealed a significant disequilibrium between brain and serum water for 15 min and between csf and serum water for 5 min after in ...	1989	2760817
protein synthesis and degradation in non-cultured and in-vitro cultured rabbit blastocysts.	in 'pulse-chase' experiments synthesis and half-lives of leucine-labelled proteins were determined in rabbit blastocysts. embryos were either non-cultured controls or were cultured for 24 h or 48 h in ham's f-10 medium supplemented with homologous serum or uterine flushings. in control blastocysts protein synthesis increased by a factor of 10 between day 4 and day 5. half-lives of newly synthesized proteins were 32 h in day-4 and 99 h in day-5 control blastocysts. in-vitro culture of day-4 blast ...	1989	2760880
growth and metabolic properties of bacteroides intermedius in anaerobic continuous culture.	two strains of bacteroides intermedius bh20/30 and bh18/23, have been grown in anaerobic continuous culture under various conditions for periods up to 54 days. strain bh20/30 grew over a relatively wide ph range from 5-8 with a maximum at ph 7.0 at a dilution rate (d) of 0.1 h-1 with a glucose limitation, while strain bh18/23 had an optimum between 5.8 and 7.3 and would not grow above and below this range. the maximum growth rate (mu max) for the latter strain was shown to be 0.23 h-1, or a doub ...	1989	2762020
molecular cloning and sequence analysis of putative chicken prolactin cdna.	a cdna library was prepared from mrna isolated from anterior pituitary glands of incubating bantam hens, in which prolactin mrna levels were predicted to be very high. nine clones, representing abundant mrna species, were identified and shown to contain homologous sequences. two clones, of 871 bp and 580 bp, were analysed by dna sequencing. the shorter clone was found to be a truncated cdna product but otherwise identical to the longer clone. the 871 bp cdna, prl101, contains an open reading fra ...	1989	2765112
endothelium-dependent inhibition of ergonovine-induced contraction is impaired in porcine coronary arteries with regenerated endothelium.	the inhibitory effects of the endothelium against ergonovine-induced contraction were examined in isolated porcine coronary arteries under normal conditions and after endothelial regeneration. endothelium-dependent responses were examined in vitro in normal yorkshire pigs (n = 16) and in pigs that had undergone balloon endothelium removal of the left anterior descending coronary artery (lad) 4 weeks before the study (n = 10). the presence of a complete endothelial lining was confirmed histologic ...	1989	2766515
smokeless tobacco use among adolescents: correlates and concurrent predictors.	seventh grade students (n = 1539) from three regions of new york state were surveyed to determine the prevalence of smokeless tobacco use and its relationship to seven background variables, 13 substance use variables, and 19 psychosocial variables. significant correlations with smokeless tobacco use were found within each of these variable domains. concurrent predictors for each domain were determined using logistic regression analysis. the resulting three models were combined in a stepwise fash ...	1989	2768485
em autoradiographic study of the projections from the dorsal nucleus of the lateral lemniscus: a possible source of inhibitory inputs to the inferior colliculus.	the fine structure of the projection from the dorsal nucleus of the lateral lemniscus (dnll) to the inferior colliculus is examined in the cat. anterograde axonal transport of 3h-leucine and em autoradiographic techniques are used to label axonal endings from dnll. the primary finding is that axonal endings from dnll contain pleomorphic synaptic vesicles and make symmetrical synaptic contacts. this morphology is associated with inhibitory synapses. the projection from dnll is the source of appro ...	1989	2768557
determination of estradiol 2- and 16-alpha-hydroxylase activities in mcf-7 human breast cancer cells in culture using radiometric analysis.	using a radiometric assay the effects of estradiol upon the activity of estradiol 2- and 16 alpha-hydroxylases in mcf-7 human breast cancer cells in culture were studied. after 5 days of treatment and 36 h of withdrawal, incubation in the presence of either 2- or 16 alpha-tritiated substrate was carried out. estradiol (10 nm) significantly increased 16 alpha-hydroxylase activity (21%, p less than 0.01), while no effects on 2-hydroxylase activity was observed. treatment for 6 weeks caused a major ...	1989	2770303
[cardiovascular risk factors in children and adolescents in fuenlabrada: objectives, features of the population, design, participation, equipment, laboratory technics, and quality control].	fuenlabrada is at the present time the most industrialized town placed at the south are of madrid. this population has some peculiar attractive characteristics from the epidemiologic point of view. perhaps the most important is that the majority of its inhabitants are immigrant from other depressed rural areas of the country, with a low socio-economic and cultural level. the new conditions of life could have changed the life expectation and the causes of morbidity and mortality of this populatio ...	1989	2772364
[chronic pyelonephritis in polycystic kidney].	the characteristics of chronic pyelonephritis are studied in 37 patients out of a total of 53 patients with proved renal polycystosis. a group of 71 patients with chronic pyelonephritis selected at random are used as a control group. the frequency of chronic pyelonephritis among the patients with renal polycystosis is 69.8%. the difference between the mean age of the patients with renal polycystosis and chronic pyelonephritis and the patients with renal polycystosis without chronic pyelonephriti ...	1989	2773465
the mobilization of ferritin iron by liver cytosol. a comparison of xanthine and nadh as reducing substrates.	considerable evidence suggests that the release of iron from ferritin is a reductive process. a role in this process has been proposed for two hepatic enzymes, namely xanthine oxidoreductase and an nadh oxidoreductase. the abilities of xanthine and nadh to serve as a source of reducing power for the enzyme-mediated release of ferritin iron (ferrireductase activity) were compared with turkey liver and rat liver homogenates. the maximal velocity (vmax.) for the reaction with nadh was 50 times grea ...	1989	2775199
omega-hydroxyacid derivatives in the epidermis of several mammalian species.	1. omega-hydroxyacids from the acylceramides and acylglucosylceramides of mammalian epidermis were examined by thin-layer and gas-liquid chromatography to determine their degree of unsaturation and chain-length distributions. the species examined included human (homo sapiens), pig (sus scrofa), mouse (mus musculus) and rat (rattus rattus). 2. within a species, the omega-hydroxyacids from the acylceramide and acylglucosylceramide were essentially identical. 3. the human omega-hydroxyacids proved ...	1989	2776425
repair of sublethal damage in two human tumor cell lines grown as multicellular spheroids.	multicellular tumor spheroids (mts) provide a suitable in vitro model to study radiation sensitivity of tumor cells. two cell lines of human origin, obtained from a neuroblastoma (nb-100) and a squamous cell carcinoma (hn-1), were exposed to graded doses (4-9 gy) of radiation with 18 mv photons. radiation was applied either as a single or as a split dose with an interval of 6 hr to determine the extent of sublethal damage repair. treated spheroids regrew at approximately the same growth rate as ...	1989	2777647
the sz mutant hamster: a genetic model of epilepsy or of paroxysmal dystonia?	attacks of sustained dystonic postures of limbs and trunk can be initiated by mild environmental stimuli in an inbred line of syrian hamsters. the trait is determined by an autosomal simple recessive genetic mutation, originally designated by the gene symbol sz, because the abnormal movements were thought to represent epileptic seizures. the attacks, which can be reproducibly initiated by placing the sz mutant hamsters in a new environment, begin with rapid twitches of the vibrissae, flattened e ...	1989	2779593
opening remarks of president fernando e. agrait on the occasion of the 50th anniversary of the cayo santiago macaque colony.		1989	2780954
[toxoplasma gondii infections and oocyst shedding in domestic cats and the significance of this for the epidemiology and epizootiology of toxoplasmosis].	because of the dissemination of oocysts the cat and other felines have a central position within toxoplasma gondii epidemiology and epizootiology. from 1984 to 1986, 267 cats between 2 months and 15 years old were serologically examined (sft) for toxoplasma antibodies. the rate of infection was 59.9 per cent. the infection rate of stray cats (72.5%) was significantly higher than that of domestic cats (40%). the high infection rate of herbivores results from behaviour of stray cats. in faeces of ...	1989	2782669
neurochemical profile of moclobemide, a short-acting and reversible inhibitor of monoamine oxidase type a.	moclobemide belongs to a new generation of short-acting, reversible, monoamine oxidase (mao) inhibitors. in vitro (rat brain homogenates) moclobemide inhibits mao-a selectively with lower potency than many of the reference mao inhibitors. however, when measured ex vivo in the rat, the potency of moclobemide is similar to that of reference compounds. in vivo the drug induces a dose-dependent, short-lasting (8-16 hr) and preferential inhibition of mao-a in the brain and both mao-a and mao-b inhibi ...	1989	2783611
neurochemical profile of moclobemide, a short-acting and reversible inhibitor of monoamine oxidase type a.	moclobemide belongs to a new generation of short-acting, reversible, monoamine oxidase (mao) inhibitors. in vitro (rat brain homogenates) moclobemide inhibits mao-a selectively with lower potency than many of the reference mao inhibitors. however, when measured ex vivo in the rat, the potency of moclobemide is similar to that of reference compounds. in vivo the drug induces a dose-dependent, short-lasting (8-16 hr) and preferential inhibition of mao-a in the brain and both mao-a and mao-b inhibi ...	1989	2783611
a novel calcium signalling response in the breast cancer cell line mda-468.	in the human breast carcinoma cell line mda-468 addition of epidermal growth factor (egf) is growth inhibitory. calcium signalling was investigated in this cell line using the calcium sensitive fluorescent probe indo-1. addition of egf to mda-468 cells resulted in a novel biphasic calcium response. in the first phase of the response egf raised calcium to levels significantly above basal. this was followed by a prolonged fall in calcium to levels significantly lower than original basal levels. th ...	1989	2784677
results of the flow cytometry actg quality control program: analysis and findings.	the aids clinical trial group's (actg) immunology committee was charged with initiating a quality control program for all laboratories participating in the actg program reporting flow cytometry data. forty-one laboratories were evaluated. this report defines the goals of this program and the subsequent findings after 19 send-outs were made. both hiv positive volunteer donors and normal age-matched donors were used. sample sets included both heparin and edta anticoagulated bloods. laboratories we ...	1989	2785890
co-localization of corticotropin-releasing factor- and enkephalin-like immunoreactivities in nerve cells of the rat hypothalamus and adjacent areas.	the co-localization of corticotropin-releasing factor (crf)- and enkephalin (enk)-like immunoreactivities in nerve cells of the rat hypothalamus and adjacent areas was investigated by the simultaneous application of immuno-beta-galactosidase staining and the peroxidase-antiperoxidase (pap) method to the same sections. crf-like immunoreactive cells were stained blue with immuno-beta-galactosidase staining and enk-like immunoreactive cells brown with the pap method. double-labeled cells with overl ...	1989	2786446
protection induced against acute bronchitis--the use of human and rat models to determine mechanisms of action of oral immunization with haemophilus influenzae.		1989	2786462
human t lymphocytes possess a limited in vitro life span.	the t lymphocyte offers certain theoretical advantages over other available cell types for the study of aging. immunosenescence is a well-established part of, and may be directly relevant to, mammalian aging, and the t lymphocyte is well-characterized as to function, cell-surface antigen make-up, and other factors. however, prior efforts at studying in vitro aging of t cells have been hampered by poor reproducibility in doubling potential and the occurrence of a peculiar type of crisis. we have ...	1989	2786475
lymphokine-activated killer (lak) cells inhibit the clonogenic growth of human leukemic stem cells.	the effect of lymphokine-activated killer (lak) cells on the in vitro clonogenic capacity of acute myeloid leukemia (aml) blasts was investigated in a semisolid medium assay. the leukemic clonogenic capacity of 11 aml cases, selected on the basis of their ability to grow in vitro, was highly reduced following overnight preincubation with lak effectors. the degree of colony inhibition, which ranged between 66% and 98% (mean 83.8% +/- 11.4 sd), was quantitatively greater than by 51cr release, whic ...	1989	2786477
acute pathological effects on rats of orally administered wortmannin-containing preparations and purified wortmannin from fusarium oxysporum.	the pathological effects in rats of orally administered wortmannin, a mycotoxin produced by fusarium oxysporum, were studied. weanling rats were fed a wortmannin-containing fungal culture for 4 or 5 days, or were given by intragastric gavage a single, lethal dose of extracts of a wortmannin-containing fungal culture or pure toxin. haemoglobinuria, necrosis of lymphoid tissues and death occurred in rats fed the fungal culture. administration by gavage of extracts of the wortmannin-containing cult ...	1989	2786490
longitudinal study of the in vivo hprt mutant frequency in human t-lymphocytes as determined by a cell cloning assay.	the in vivo frequency of mutants resulting from mutation at the hprt locus in human t-lymphocytes can be determined by a cloning assay. this assay quantifies the frequency of 6-thioguanine-resistant (tgr) t-cells through growth of colonies in 96-well microtiter dishes. the reproducibility of the tgr mutant frequency values has now been assessed in a longitudinal study of six individuals (three male, three female, aged 22-33 years) employing 4-5 blood samples over a 26-37 week time period. clonin ...	1989	2786809
inhibition of enteropathogenic bacteria by human milk whey in vitro.	diarrhea caused by enteropathogenic bacteria is a leading cause of childhood mortality world-wide, particularly in less developed regions. breast-feeding has been advocated to protect infants and children from infectious illnesses. we examined the antibacterial activity of human whey in vitro against multiple strains of the following species of enteropathogenic bacteria, campylobacter jejuni, escherichia coli, salmonella typhimurium, shigella flexneri, shigella sonnei and vibrio cholerae, all is ...	1989	2787902
variceal hemorrhage as a consequence of spontaneous rupture of a pancreatic pseudocyst into the splenic vein.	we describe a case of a 68-yr-old man who died as a consequence of a massive bleed from esophageal varices. at autopsy, a pancreatic pseudocyst that communicated directly with the splenic vein was found. histology confirmed that material from the pseudocyst had embolized to the peripheral portal veins, occluding many of them.	1989	2788993
interleukin 1 in rheumatoid arthritis: potentiation of immune responses within the joint.	specific immunoassays were used to measure il-1 peptides in the serum and synovial fluid of patients with rheumatoid arthritis (ra) and in the serum of age-matched healthy controls. patients with ra had raised levels of both il-1 beta and il-1 alpha in their sera compared to controls. synovial fluid levels of il-1 beta significantly correlated with immunoreactive il-2 and soluble il-2 receptor (sil-2r). in addition, incubation of synovial fluid mnc with human recombinant (hr) il-1 caused a dose- ...	1989	2789325
estradiol-stimulated increases in uterine eosinophils and nuclear type ii estrogen-binding sites are prevented by pertussis toxin.	previous studies from several laboratories have demonstrated that estradiol treatment resulted in an increase in nuclear type ii binding sites. our previous data suggest that this increase was due to the estradiol-stimulated influx of circulating eosinophils. therefore, we suggested that the uterine nuclear type ii estrogen-binding sites were not of uterine origin. in this report we present further evidence to support this hypothesis. treatment of immature rats with estradiol resulted in the sti ...	1989	2792007
effects of soft contacts of differing thickness on corneal wound healing in rabbits.	this study was undertaken to determine the effects of thin (60 microns) and thick (240 microns) soft contact lenses of equal water content (70%) and power on nonlesioned and lesioned rabbit corneas. in one group of animals, corneas were not lesioned. thin lenses were placed on left corneas and thick lenses on right corneas. in a second group, lesions were made in both corneas. left corneas were covered with thin lenses and right corneas with thick lenses. post-treatment times were 8 hr and 24 hr ...	1989	2793355
detoxification of lithocholic acid. elucidation of the pathways of oxidative metabolism in rat liver microsomes.	the hydroxylation of lithocholic acid (3 alpha-hydroxy-5 beta-cholanoic acid) by adult male sprague-dawley rat liver microsomes supplemented with nadph was studied. metabolites were separated by a combination of thin-layer chromatography and high pressure liquid chromatography, both with and without prior methylation and acetylation of the samples. the resulting products were characterized by thin-layer, gas-liquid, and high pressure liquid chromatography by comparison with authentic bile acid s ...	1989	2794781
placental weights of normal and aneuploid early human fetuses.	placental and body weights were plotted for 252 normal human fetuses less than 600 gm and mean regressions and 95% prediction intervals were calculated. placentas from 62 aneuploid fetuses were compared to these standards. placenta to body weight ratios from 34 trisomy-21 fetuses were not reduced but those from 15 trisomy-18 fetuses were generally decreased. one of 5 trisomy-13 ratios was below the prediction interval. among 7 triploid fetuses the ratios were above the normal interval in 3 and b ...	1989	2798268
placental weights of normal and aneuploid early human fetuses.	placental and body weights were plotted for 252 normal human fetuses less than 600 gm and mean regressions and 95% prediction intervals were calculated. placentas from 62 aneuploid fetuses were compared to these standards. placenta to body weight ratios from 34 trisomy-21 fetuses were not reduced but those from 15 trisomy-18 fetuses were generally decreased. one of 5 trisomy-13 ratios was below the prediction interval. among 7 triploid fetuses the ratios were above the normal interval in 3 and b ...	1989	2798268
regional distribution and behavior of the subcutaneous tissue concerning selection and indication for liposuction.	the distribution of the fat tissue in the subcutaneous layer is described. since it has specific characteristics in each region of the human body, careful evaluation of the distribution before selecting patients for surgery is necessary. the subcutaneous tissue after an operation frequently demonstrates a thick and hard fibrosis which is evidence that liposuction should be performed in the lamellar layers. this type of fibrotic tissue in the subcutaneous areola layer often brings on irregulariti ...	1989	2801296
regional distribution and behavior of the subcutaneous tissue concerning selection and indication for liposuction.	the distribution of the fat tissue in the subcutaneous layer is described. since it has specific characteristics in each region of the human body, careful evaluation of the distribution before selecting patients for surgery is necessary. the subcutaneous tissue after an operation frequently demonstrates a thick and hard fibrosis which is evidence that liposuction should be performed in the lamellar layers. this type of fibrotic tissue in the subcutaneous areola layer often brings on irregulariti ...	1989	2801296
presence of cytoadhesins (iib-iiia-like glycoproteins) on human metastatic melanomas but not on benign melanocytes.	glycoproteins iib and iiia, a heterodimer complex, play a vital role in blood platelet aggregation and are members of a wide family of membrane receptors known as integrins or cytoadhesins. cellular interaction to extracellular matrix (ecm) adhesive proteins is mediated by integrins. certain tumor cells are known to interact with ecm and blood platelets in the process of metastasis. however, it is not known if tumor cells, compared with their normal counterparts, acquire iib-iiia-like receptors ...	1989	2801613
prevention of rectal cancer induced by n-nitrosobis(2-oxopropyl)-amine by exogenous testosterone in mrc rats.	rectal carcinogenicity of n-nitrosobis(2-oxopropyl)amine (bop) in male mrc wistar rats was shown to be inhibited by exogenous testosterone (t) when the hormone was given during, but not after, administration of the carcinogen. this effect was independent of the dose and frequency of bop, which was given either weekly for 20 weeks orally or daily for 3 days subcutaneously. since, except for prostatic cancer, the incidence and the patterns of other bop-induced tumors were not altered by t, this ho ...	1989	2805225
prevention of rectal cancer induced by n-nitrosobis(2-oxopropyl)-amine by exogenous testosterone in mrc rats.	rectal carcinogenicity of n-nitrosobis(2-oxopropyl)amine (bop) in male mrc wistar rats was shown to be inhibited by exogenous testosterone (t) when the hormone was given during, but not after, administration of the carcinogen. this effect was independent of the dose and frequency of bop, which was given either weekly for 20 weeks orally or daily for 3 days subcutaneously. since, except for prostatic cancer, the incidence and the patterns of other bop-induced tumors were not altered by t, this ho ...	1989	2805225