Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| molecular basis of e. coli l-threonine aldolase catalytic inactivation at low ph. | l-threonine aldolases (tas), a family of enzymes belonging to the fold-type i pyridoxal 5'-phosphate (plp) dependent enzymes, play a role in catalyzing the reversible cleavage of l-3-hydroxy-α-amino acids to glycine and the corresponding aldehydes. threonine aldolases have great biotechnological potential for the syntheses of pharmaceutically relevant drug molecules because of their stereospecificity. the ph-dependency of their catalytic activity, affecting reaction intermediates, led us to stud ... | 2015 | 25560296 |
| short-term prophylaxis with ciprofloxacin in extended 16-core prostate biopsy. | to evaluate the safety, efficacy and possible complications of 16-core transrectal prostate biopsies using two doses of ciprofloxacin for prophylaxis of infectious complications. | 2015 | 25928510 |
| role of bacteria in oral carcinogenesis. | oral cancer appears to be increasing in incidence, and mortality has hardly improved over the past 25 years. better understanding of the etiopathogenesis should lead to more accurate and earlier diagnosis and more effective treatments with fewer adverse effects. despite increasing interest in the possible relationships between bacteria and the different stages of cancer development, the association of bacteria with cancer of the oral cavity has yet to be adequately examined. different bacteria h ... | 2015 | 25767359 |
| appendicitis in a child due to streptococcus pneumoniae: a rare case report. | a variety of bacterial species play a major role in appendicitis. both aerobic and anaerobic gram positive and gram negative bacteria such as, bacteroides fragilis, streptococcus species, escherichia coli, klebsiella species and citrobacter freundii cause appendicitis. appendicitis is usually polymicrobial. the case assumes importance because of single aetiological agent i.e. unimicrobial and no predisposing factors are present to cause infection. we report a rare case of appendicitis due to str ... | 2015 | 25737990 |
| galacto-oligosaccharides and colorectal cancer: feeding our intestinal probiome. | prebiotics are ingredients selectively fermented by the intestinal microbiota that promote changes in the microbial community structure and/or their metabolism, conferring health benefits to the host. studies show that β (1-4) galacto-oligosaccharides [β (1-4) gos], lactulose and fructo-oligosaccharides increase intestinal concentration of lactate and short chain fatty acids, and stool frequency and weight, and they decrease fecal concentration of secondary bile acids, fecal ph, and nitroreducta ... | 2015 | 25584074 |
| fast and continuous microorganism detection using aptamer-conjugated fluorescent nanoparticles on an optofluidic platform. | fast and accurate pathogen detection in aquatic environments is challenging in many biomedical studies and microbial diagnostic applications. in this study, we developed a real-time, continuous, and non-destructive single cell detection method using target specific aptamer-conjugated fluorescent nanoparticles (a-fnps) and an optofluidic particle-sensor platform. a-fnps selectively bound to the surfaces of target bacteria (escherichia coli) and labeled them with high affinity and selectivity so t ... | 2015 | 25190089 |
| incidence of fecal enterobacteriaceae producing broad-spectrum beta-lactamases in patients with hematological malignancies. | given the steadily increasing numbers of resistant bacteria, the frequency and severity of infections are on the rise. in patients with hematological malignancies, the treatment itself increases the risk of complicating bacterial infections. one important mechanisms of resistance is production of broad-spectrum beta-lactamases, increasingly detected not only in bacterial pathogens but also in bacteria contained in the normal microflora of the human body. the objectives of this study were determi ... | 2015 | 25270106 |
| microbiological study of therapeutic soft contact lenses used in the treatment of recurrent corneal erosion syndrome. | to determine the bacteriological spectrum of the removed therapeutic soft contact lenses (tscls) and to establish efficacy of prophylactic antibiotics on tscls used for 2 weeks for treatment of patients with recurrent corneal erosion syndrome (rces). | 2015 | 25230080 |
| [recurrent meningitis due to anatomical defects: the bacteria indicates its origin]. | recurrent meningitis is a rare disease. anatomical abnormalities and immunodeficiency states are predisposing factors. four cases, in which immunodeficiency was excluded, are presented. the causal microorganism led to the detection of the anatomical defect responsible for the recurrences. | 2015 | 25446794 |
| 1,3-propanediol production by new recombinant escherichia coli containing genes from pathogenic bacteria. | 1,3-propanediol (1,3-pdo) is an organic compound, which is a valuable intermediate product, widely used as a monomer for synthesizing biodegradable polymers, increasing their strength; as well as an ingredient of textile, cosmetic and medical products. 1,3-pdo is mostly synthesized chemically. global companies have developed technologies for 1,3-pdo synthesis from petroleum products such as acrolein and ethylene oxide. a potentially viable alternative is offered by biotechnological processes usi ... | 2015 | 25644946 |
| concurrent interspecies and clonal dissemination of oxa-48 carbapenemase. | several isolates of four different carbapenemase-producing enterobacteriaceae species were recovered from a patient hospitalized for 4 months in a teaching hospital in madrid. these species comprised seven klebsiella pneumoniae belonging to st15, four escherichia coli belonging to st2531, two serratia marcescens and one citrobacter freundii. this patient was the index case of a small outbreak of four patients infected and/or colonized by carbapenemase-producing k. pneumoniae. molecular results i ... | 2015 | 25596781 |
| the role of active site tyrosine 58 in citrobacter freundii methionine γ-lyase. | in the spatial structure of methionine γ-lyase (mgl, ec 4.4.1.11) from citrobacter freundii, tyr58 is located at h-bonding distance to the oxygen atom of the phosphate "handle" of pyridoxal 5'-phosphate (plp). it was replaced for phenylalanine by site-directed mutagenesis. the x-ray structure of the mutant enzyme was determined at 1.96å resolution. comparison of spatial structures and absorption spectra of wild-type and mutant holoenzymes demonstrated that the replacement did not result in essen ... | 2015 | 25584856 |
| shikimic acid, a base compound for the formulation of swine/avian flu drug: statistical optimization, fed-batch and scale up studies along with its application as an antibacterial agent. | the sudden outbreak of swine flu has increased the global demand of shikimic acid which is an industrially interesting compound, as it is used as a key starting material for the synthesis of a neuraminidase inhibitor tamiflu(®), for the treatment of antiviral infections such as swine flu. statistical optimization and evaluation of medium components for the production of shikimic acid by citrobacter freundii is addressed in the present investigation. plackett-burman design was applied for the scr ... | 2015 | 25563634 |
| [antimicrobial resistance monitoring of gram-negative bacilli isolated from 15 teaching hospitals in 2014 in china]. | to investigate the current situation of antimicrobial resistance of nosocomial gram-negative bacilli in 2014 in china. | 2015 | 26675021 |
| comparative evaluation of the hydrogen production by mixed consortium, synthetic co-culture and pure culture using distillery effluent. | wastewater comprises of various carbon sources. so, the use of microbial consortium may improve the hydrogen production and organic reduction. the present study deals with biohydrogen production by acidogenic mixed consortia (amc), synthetic co-culture (klebsiella pneumoniae iit-bt 08 and citrobacter freundii iit-bt l139) and pure culture using distillery effluent (de). higher hydrogen yield was observed in case of amc (9.17 mol/kg codreduced) as compared to the synthetic co-culture and pure cul ... | 2015 | 26433784 |
| 1,3-propanediol production by escherichia coli using genes from citrobacter freundii atcc 8090. | compared with chemical synthesis, fermentation has the advantage of mass production at low cost, and has been used in the production of various industrial chemicals. as a valuable organic compound, 1,3-propanediol (1,3-pdo) has numerous applications in the production of polymers, lubricants, cosmetics and medicines. here, conversion of glycerol (a renewable substrate and waste from biodiesel production) to 1,3-pdo by e. coli bacterial strain carrying altered glycerol metabolic pathway was invest ... | 2015 | 26345096 |
| factors affecting the activation and inhibition of intracellular enzymes for degradation of 1,2 diamino benzene: kinetics and thermodynamic studies. | citrobacter freundii, the bacterium isolated from marine sediments was capable of degrading 1,2 diamino benzene (dab), an endocrine disruptor. the mixed intracellular enzymes from c. freundii were extracted and purified. the mixed intracellular enzymes were used for the degradation of dab and degree of degradation was evaluated in terms of pyruvic acid, the end product, formed. the variables such as effect of ph, temperature and metal ions on the degradation of dab using mixed intracellular enzy ... | 2015 | 26334986 |
| in vivo horizontal dissemination of the blakpc-2 gene carried on diverse genetic platforms among clinical isolates of enterobacteriaceae. | this study investigated the molecular characteristics of six blakpc-positive enterobacteriaceae recovered from three patients in argentina. antimicrobial susceptibility testing was performed following clinical and laboratory standards institute (clsi) 2014 recommendations. molecular characterisation of the isolates was performed by biparental conjugation, pcr, sequencing, s1 nuclease restriction, and southern blot hybridisation with a blakpc probe using standard protocols and conditions. the iso ... | 2015 | 27873711 |
| coexistence of a novel kpc-2-encoding mdr plasmid and an ndm-1-encoding pndm-hn380-like plasmid in a clinical isolate of citrobacter freundii. | the objective of this study was to characterize the molecular mechanism of coproduction of kpc-2 and ndm-1 in citrobacter freundii. | 2015 | 26260129 |
| acute death associated with citrobacter freundii infection in an african elephant (loxodonta africana). | a 21-year-old male african elephant (loxodonta africana) died suddenly with no previous medical history. grossly, there were severe multifocal epicardial and endocardial hemorrhages of the atria and ventricles, hydropericardium, multifocal pleural hemorrhages, and severe pulmonary congestion and edema. histologically, there was fibrinoid vasculitis and thrombosis in the heart and lung and myocardial necrosis. citrobacter freundii was isolated in abundance in pure culture from liver and heart sam ... | 2015 | 26179092 |
| characterization of chromosomal qnrb and ampc alleles in citrobacter freundii isolates from different origins. | the association of esbls (extended-spectrum beta-lactamases)/pampcs (plasmid-mediated ampc β-lactamases) with pmqr (plasmid mediated quinolone resistance) in gram-negative bacteria has been of great concern. the present study was performed to characterize the diversity, gene location, genetic context, and evolution of ampc and qnrb alleles in isolates of citrobacter freundii. fifteen isolates of c. freundii were identified from a total of 788 isolates of enterobacteriaceae derived from humans, a ... | 2015 | 26168881 |
| oxa-372, a novel carbapenem-hydrolysing class d β-lactamase from a citrobacter freundii isolated from a hospital wastewater plant. | the objective of this study was to characterize a novel class d carbapenemase, named oxa-372, identified in a carbapenem-resistant citrobacter freundii strain (cfr-fi-07) isolated from a hospital wastewater plant in central italy. | 2015 | 26126492 |
| application of bacteriophages to reduce biofilms formed by hydrogen sulfide producing bacteria on surfaces in a rendering plant. | hydrogen sulfide producing bacteria (spb) in raw animal by-products are likely to grow and form biofilms in the rendering processing environments, resulting in the release of harmful hydrogen sulfide (h2s) gas. the objective of this study was to reduce spb biofilms formed on different surfaces typically found in rendering plants by applying a bacteriophage cocktail. using a 96-well microplate method, we determined that 3 spb strains of citrobacter freundii and hafnia alvei are strong biofilm for ... | 2015 | 26102989 |
| experimental application of lactobacillus fermentum ccm 7421 in combination with chlorophyllin in dogs. | chlorophyll belongs in a larger class of phytochemical plant pigments currently receiving more attention as a physiologically active dietary component. although most research has focused on its biological activities such as its antioxidant, antimutagenic, anti-inflammatory or apoptotic effects in humans or rodents, there is limited knowledge at this time about the combinative possibilities of chlorophyll with probiotic bacteria. our aim was to test the growth characteristics of canine-derived pr ... | 2015 | 26062536 |
| mass mortality in ornamental fish, cyprinus carpio koi caused by a bacterial pathogen, proteus hauseri. | moribund koi carp, cyprinus carpio koi, from a farm with 50% cumulative mortality were sampled with the aim of isolating and detecting the causative agent. three bacterial species viz., citrobacter freundii (nscf-1), klebsiella pneumoniae (nskp-1) and proteus hauseri [genomospecies 3 of proteus vulgaris bio group 3] (nsph-1) were isolated, identified and characterized on the basis of biochemical tests and sequencing of the 16s rdna gene using universal bacterial primers. challenge experiments wi ... | 2015 | 26028178 |
| acute cholecystitis associated with infection of enterobacteriaceae from gut microbiota. | acute cholecystitis (ac) is one of the most common surgical diseases. bacterial infection accounts for 50% to 85% of the disease's onset. since there is a close relationship between the biliary system and the gut, the aims of this study were to characterize and determine the influence of gut microbiota on ac, to detect the pathogenic microorganism in the biliary system, and to explore the relationship between the gut and bile microbiota of patients with ac. a total of 185 713 high-quality sequen ... | 2015 | 26025761 |
| casque infection, resolution, and subsequent repneumatization in a trumpeter hornbill (bycanistes buccinator). | 2015 | 25909990 | |
| [nmr screening of potential inhibitors of citrobacter freundii methionine]. | methionine γ-lyase [ec 4.4.1.11] participates in a methionine catabolism at a number of bacteria and protozoa eukaryotes, including pathogenic microorganisms. lack of this enzyme at mammals allows consider it as a perspective target for rational antibacterial drug design. currently in medical practice there are no the preparations based on an inhibition of methionine γ-lyase activity. we present results of the search of potential inhibitors of the enzyme using the nmr screening techniques based ... | 2015 | 25845243 |
| prevalence and characterization of esbl- and ampc-producing enterobacteriaceae on retail vegetables. | in total 1216 vegetables obtained from dutch stores during 2012 and 2013 were analysed to determine the prevalence of 3rd-generation cephalosporin (3gc) resistant bacteria on soil-grown fresh produce possibly consumed raw. vegetables grown conventionally and organically, from dutch as well as foreign origin were compared. included were the following vegetable types; blanched celery (n=192), bunched carrots (n=190), butterhead lettuce (n=137), chicory (n=96), endive (n=188), iceberg lettuce (n=19 ... | 2015 | 25828704 |
| antibacterial susceptibility of enterobacteriaceae isolated from raw horsemeat isolated for human consumption (basashi). | drug susceptibility testing was carried out using 14 antibiotics in order to identify trends in the antibiotic tolerance of 142 strains of enterobacteriaceae isolated from horsemeat commercially available for raw consumption (basashi). a comparison of the sensitivity to the 14 antibiotics using the 90% mic (minimum inhibitory concentration) values (mic90) showed the strongest tolerance to ampicillin (abpc) at a concentration of > 128 μg/ml, followed by that to fosfomycin (fom) at a concentration ... | 2015 | 25817809 |
| detection of extended-spectrum beta-lactamase (esbl)-producing enterobacteriaceae in vegetables, soil and water of the farm environment in tunisia. | one-hundred-nine samples of 18 different farms (49 of food-vegetables, 41 of soil and 19 of irrigation water) and 45 vegetable food samples of 13 markets were collected in tunisia. these samples were inoculated in macconkey agar plates supplemented with cefotaxime (2 μg/ml). esbl-producing enterobacteriaceae (esbl-eb) were detected in 10 of the 109 farm samples (vegetables, 8.2%; soil, 7.3%; water, 15.8%), and in 4 of 45 vegetables of markets (8.9%), recovering 15 esbl-eb. isolates and esbl gene ... | 2015 | 25791254 |
| tracing enteric pathogen contamination in sub-saharan african groundwater. | quantitative pcr (qpcr) can rapidly screen for an array of faecally-derived bacteria, which can be employed as tracers to understand groundwater vulnerability to faecal contamination. a microbial dna qpcr array was used to examine 45 bacterial targets, potentially relating to enteric pathogens, in 22 groundwater supplies beneath the city of kabwe, zambia in both the dry and subsequent wet season. thermotolerant (faecal) coliforms, sanitary risks, and tryptophan-like fluorescence, an emerging rea ... | 2015 | 26363144 |
| identification and inhibition of histamine-forming bacteria in blue scad (decapterus maruadsi) and chub mackerel (scomber japonicus). | in this study, we investigated the differences in histamine accumulation between blue scad and chub mackerel and methods of inhibiting histamine-forming bacteria and controlling histamine accumulation in fish. the free histidine contents in blue scad and chub mackerel were 1.45 and 2.75 mg/g, respectively. the histamine-forming bacteria isolated from them were identified as citrobacter freundii, citrobacter braakii, and enterobacter aerogenes using 16s rdna sequence analysis, the vitek 2 compact ... | 2015 | 25710155 |
| characterization of beta-lactamases in faecal enterobacteriaceae recovered from healthy humans in spain: focusing on ampc polymorphisms. | the intestinal tract is a huge reservoir of enterobacteriaceae, some of which are opportunist pathogens. several genera of these bacteria harbour intrinsic antibiotic resistance genes, such as ampc genes in species of citrobacter, enterobacter or escherichia genera. in this work, beta-lactamases and other resistance mechanisms have been characterized in enterobacteriaceae isolates recovered from healthy human faecal samples, focusing on the ampc beta-lactamase genes. fifty human faecal samples w ... | 2015 | 25501887 |
| a plasmid-transposon hybrid mutagenesis system effective in a broad range of enterobacteria. | random transposon mutagenesis is a powerful technique used to generate libraries of genetic insertions in many different bacterial strains. here we develop a system facilitating random transposon mutagenesis in a range of different gram-negative bacterial strains, including pectobacterium atrosepticum, citrobacter rodentium, serratia sp. atcc39006, serratia plymuthica, dickeya dadantii, and many more. transposon mutagenesis was optimized in each of these strains and three studies are presented t ... | 2015 | 26733980 |
| directed evolution and resolution mechanism of 1, 3-propanediol oxidoreductase from klebsiella pneumoniae toward higher activity by error-prone pcr and bioinformatics. | 1, 3-propanediol oxidoreductase (pdor) is a key enzyme in glycerol bioconversion to 1,3-propanediol (1, 3-pd) which is a valuable chemical and one of the six new petrochemical products. we used error-prone pcr and activity screening to identify mutants of klebsiella pneumoniae (k. pneumoniae) pdor with improved activity. the activity of one of the identified mutants, pdor'-24, which includes a single mutation, a199s, was 48 u/mg, 4.9 times that of the wild-type enzyme. molecular docking was perf ... | 2015 | 26528716 |
| violacein: properties and production of a versatile bacterial pigment. | violacein-producing bacteria, with their striking purple hues, have undoubtedly piqued the curiosity of scientists since their first discovery. the bisindole violacein is formed by the condensation of two tryptophan molecules through the action of five proteins. the genes required for its production, vioabcde, and the regulatory mechanisms employed have been studied within a small number of violacein-producing strains. as a compound, violacein is known to have diverse biological activities, incl ... | 2015 | 26339614 |
| high crude violacein production from glucose by escherichia coli engineered with interactive control of tryptophan pathway and violacein biosynthetic pathway. | as bacteria-originated crude violacein, a natural indolocarbazole product, consists of violacein and deoxyviolacein, and can potentially be a new type of natural antibiotics, the reconstruction of an effective metabolic pathway for crude violacein (violacein and deoxyviolacein mixture) synthesis directly from glucose in escherichia coli was of importance for developing industrial production process. | 2015 | 25592762 |
| invasive group b streptococcal infection in infants in shenzhen, china. | in this study, we aim to investigate the distribution and antibiotic susceptibility of group b streptococcus (gbs) in infants younger than 90 days in shenzhen, china. | 2015 | 25932259 |
| epidemiology and characteristics of urinary tract infections in children and adolescents. | urinary tract infections (utis) are among the most common infections in the pediatric population. over the last two decades, antibiotic resistance is increasing significantly as extended spectrum beta lactamase (esbl) producing organisms are emerging. the aim of this study is to provide a comprehensive view of the epidemiologic characteristics of utis in hospitalized children, examine the risk factors of utis caused by esbl-producing organisms, and determine the resistance patterns in the isolat ... | 2015 | 26075187 |
| antibacterial activity of ovary extract from sea urchin diadema setosum. | sea urchin gonad is considered as a highly prized delicacy in several countries. it is also rich in valuable bioactive compounds including polyunsaturated fatty acids (pufas) and β-carotene. this study was undertaken to examine the antimicrobial properties of the ovary extract from sea urchin diadema setosum against selected gram-negative and gram-positive bacteria. | 2015 | 26044237 |
| in vitro activity of ceftazidime, ceftaroline and aztreonam alone and in combination with avibactam against european gram-negative and gram-positive clinical isolates. | recent clinical isolates of key gram-negative and gram-positive bacteria were collected in 2012 from hospitalised patients in medical centres in four european countries (france, germany, italy and spain) and were tested using standard broth microdilution methodology to assess the impact of 4 mg/l avibactam on the in vitro activities of ceftazidime, ceftaroline and aztreonam. against enterobacteriaceae, addition of avibactam significantly enhanced the level of activity of these antimicrobials. mi ... | 2015 | 25748553 |
| chemical composition and antibacterial activity of lavandula coronopifolia essential oil against antibiotic-resistant bacteria. | the aim of this study was to analyse the composition of the essential oil (eo) of lavandula coronopifolia from morocco and to evaluate its in vitro antibacterial activity against antibiotic-resistant bacteria isolated from clinical infections. the antimicrobial activity was assessed by a broth micro-well dilution method using multiresistant clinical isolates of 11 pathogenic bacteria: klebsiella pneumoniae subsp. pneumoniae, klebsiella ornithinolytica, escherichia coli, enterobacter cloacae, ent ... | 2015 | 25174508 |
| a rare case of ecthyma gangrenosum caused by proteus vulgaris and candida albicans in a patient with castleman disease. | 2015 | 26618137 | |
| antimicrobial heteroresistance: an emerging field in need of clarity. | "heteroresistance" describes a phenomenon where subpopulations of seemingly isogenic bacteria exhibit a range of susceptibilities to a particular antibiotic. unfortunately, a lack of standard methods to determine heteroresistance has led to inappropriate use of this term. heteroresistance has been recognized since at least 1947 and occurs in gram-positive and gram-negative bacteria. its clinical relevance may be considerable, since more resistant subpopulations may be selected during antimicrobi ... | 2015 | 25567227 |
| identification of dha-23, a novel plasmid-mediated and inducible ampc beta-lactamase from enterobacteriaceae in northern taiwan. | ampc β-lactamases are classified as amber class c and bush group 1. ampc β-lactamases can hydrolyze broad and extended-spectrum cephalosporins, and are not inhibited by β-lactamase inhibitors such as clavulanic acid. this study was conducted to identify dha-23, a novel plasmid-mediated and inducible ampc β-lactamase obtained from enterobacteriaceae. | 2015 | 25999942 |
| structure, function, and assembly of adhesive organelles by uropathogenic bacteria. | bacteria assemble a wide range of adhesive proteins, termed adhesins, to mediate binding to receptors and colonization of surfaces. for pathogenic bacteria, adhesins are critical for early stages of infection, allowing the bacteria to initiate contact with host cells, colonize different tissues, and establish a foothold within the host. the adhesins expressed by a pathogen are also critical for bacterial-bacterial interactions and the formation of bacterial communities, including biofilms. the a ... | 2015 | 26542038 |
| identification and molecular characterisation of a novel mu-like bacteriophage, sfmu, of shigella flexneri. | s. flexneri is the leading cause of bacillary dysentery in the developing countries. several temperate phages originating from this host have been characterised. however, all s. flexneri phages known to date are lambdoid phages, which have the ability to confer the o-antigen modification of their host. in this study, we report the isolation and characterisation of a novel mu-like phage from a serotype 4a strain of s. flexneri. the genome of phage sfmu is composed of 37,146 bp and is predicted to ... | 2015 | 25902138 |
| non-protein coding rna genes as the novel diagnostic markers for the discrimination of salmonella species using pcr. | salmonellosis, a communicable disease caused by members of the salmonella species, transmitted to humans through contaminated food or water. it is of paramount importance, to generate accurate detection methods for discriminating the various salmonella species that cause severe infection in humans, including s. typhi and s. paratyphi a. here, we formulated a strategy of detection and differentiation of salmonellosis by a multiplex polymerase chain reaction assay using s. typhi non-protein coding ... | 2015 | 25774907 |
| structural basis for carbapenem-hydrolyzing mechanisms of carbapenemases conferring antibiotic resistance. | carbapenems (imipenem, meropenem, biapenem, ertapenem, and doripenem) are β-lactam antimicrobial agents. because carbapenems have the broadest spectra among all β-lactams and are primarily used to treat infections by multi-resistant gram-negative bacteria, the emergence and spread of carbapenemases became a major public health concern. carbapenemases are the most versatile family of β-lactamases that are able to hydrolyze carbapenems and many other β-lactams. according to the dependency of dival ... | 2015 | 25938965 |
| survey and rapid detection of klebsiella pneumoniae in clinical samples targeting the rcsa gene in beijing, china. | klebsiella pneumoniae is a wide-spread nosocomial pathogen. a rapid and sensitive molecular method for the detection of k. pneumoniae in clinical samples is needed to guide therapeutic treatment. in this study, we first described a loop-mediated isothermal amplification (lamp) method for the rapid detection of capsular polysaccharide synthesis regulating gene rcsa from k. pneumoniaein clinical samples by using two methods including real-time turbidity monitoring and fluorescence detection to ass ... | 2015 | 26052327 |
| development of a custom-designed, pan genomic dna microarray to characterize strain-level diversity among cronobacter spp. | cronobacter species cause infections in all age groups; however neonates are at highest risk and remain the most susceptible age group for life-threatening invasive disease. the genus contains seven species:cronobacter sakazakii, cronobacter malonaticus, cronobacter turicensis, cronobacter muytjensii, cronobacter dublinensis, cronobacter universalis, and cronobacter condimenti. despite an abundance of published genomes of these species, genomics-based epidemiology of the genus is not well establ ... | 2015 | 25984509 |
| bioavailability of dietary polyphenols and gut microbiota metabolism: antimicrobial properties. | polyphenolic compounds are plant nutraceuticals showing a huge structural diversity, including chlorogenic acids, hydrolyzable tannins, and flavonoids (flavonols, flavanones, flavan-3-ols, anthocyanidins, isoflavones, and flavones). most of them occur as glycosylated derivatives in plants and foods. in order to become bioactive at human body, these polyphenols must undergo diverse intestinal transformations, due to the action of digestive enzymes, but also by the action of microbiota metabolism. ... | 2015 | 25802870 |
| perioperative microbiologic monitoring of sputum on postoperative day one as a predictor of pneumonia after hepatectomy. | the purpose of this study was to retrospectively evaluate microbial examination of sputum on postoperative day one (pod1) and to determine risk factors for postoperative pneumonia (pop) after hepatectomy. | 2015 | 26105551 |
| point-of-care multiplex pcr promises short turnaround times for microbial testing in hospital-acquired pneumonia--an observational pilot study in critical ill patients. | the early beginning of an adequate antibiotic therapy is crucial in hospital-acquired pneumonia (hap), but depends on the results of conventional microbiological diagnostics (cmd). it was the aim of this study to evaluate the performance and turnaround times of a new point-of-care multiplex polymerase chain reaction (mpcr) system for rapid identification of pathogens and antibiotic resistance markers. we assessed the applicability of the system under real-life conditions in critical ill patients ... | 2015 | 26071191 |
| ceftaroline activity against bacterial pathogens frequently isolated in u.s. medical centers: results from five years of the aware surveillance program. | a total of 84,704 isolates were collected from 191 medical centers in 2009 to 2013 and tested for susceptibility to ceftaroline and comparator agents by broth microdilution methods. ceftaroline inhibited all staphylococcus aureus isolates at ≤2 μg/ml and was very active against methicillin-resistant strains (mic at which 90% of the isolates tested are inhibited [mic90], 1 μg/ml; 97.6% susceptible). among streptococcus pneumoniae isolates, the highest ceftaroline mic was 0.5 μg/ml, and ceftarolin ... | 2015 | 25645844 |
| antimicrobial susceptibilities of aerobic and facultative gram-negative bacilli from intra-abdominal infections in patients from seven regions in china in 2012 and 2013. | to evaluate the antimicrobial susceptibility of gram-negative bacilli that caused hospital-acquired and community-acquired intra-abdominal infections (iais) in china between 2012 and 2013, we determined the susceptibilities to 12 antimicrobials and the extended-spectrum β-lactamase (esbl) statuses of 3,540 iai isolates from seven geographic areas in china in a central laboratory using clsi broth microdilution and interpretive standards. most infections were caused by escherichia coli (46.3%) and ... | 2015 | 26482308 |
| antimicrobial susceptibilities of aerobic and facultative gram-negative bacilli from intra-abdominal infections in patients from seven regions in china in 2012 and 2013. | to evaluate the antimicrobial susceptibility of gram-negative bacilli that caused hospital-acquired and community-acquired intra-abdominal infections (iais) in china between 2012 and 2013, we determined the susceptibilities to 12 antimicrobials and the extended-spectrum β-lactamase (esbl) statuses of 3,540 iai isolates from seven geographic areas in china in a central laboratory using clsi broth microdilution and interpretive standards. most infections were caused by escherichia coli (46.3%) and ... | 2015 | 26482308 |
| prevalence of urinary tract infection and antimicrobial susceptibility among diabetic patients with controlled and uncontrolled glycemia in kuwait. | diabetic patients have higher risk of urinary tract infection (uti). in the present study, we investigated the impact of glycemic control in diabetic patients on uti prevalence, type of strains, and their antimicrobial drugs susceptibility. this study was conducted on urine samples from 722 adult diabetic patients from which 252 (35%) samples were positive for uropathogens. most uti cases occurred in the uncontrolled glycemic group (197 patients) versus 55 patients with controlled glycemia. high ... | 2015 | 26844231 |
| prevalence of urinary tract infection and antimicrobial susceptibility among diabetic patients with controlled and uncontrolled glycemia in kuwait. | diabetic patients have higher risk of urinary tract infection (uti). in the present study, we investigated the impact of glycemic control in diabetic patients on uti prevalence, type of strains, and their antimicrobial drugs susceptibility. this study was conducted on urine samples from 722 adult diabetic patients from which 252 (35%) samples were positive for uropathogens. most uti cases occurred in the uncontrolled glycemic group (197 patients) versus 55 patients with controlled glycemia. high ... | 2015 | 26844231 |
| clinical performance of a matrix-assisted laser desorption ionization-time of flight mass spectrometry method for detection of certain blakpc-containing plasmids. | rapid detection of blakpc-containing organisms can significantly impact infection control and clinical practices, as well as therapeutic choices. current molecular and phenotypic methods to detect these organisms, however, require additional testing beyond routine organism identification. in this study, we evaluated the clinical performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) to detect pkpqil_p019 (p019)-an ∼11,109-da protein associated ... | 2015 | 26338858 |
| clinical performance of a matrix-assisted laser desorption ionization-time of flight mass spectrometry method for detection of certain blakpc-containing plasmids. | rapid detection of blakpc-containing organisms can significantly impact infection control and clinical practices, as well as therapeutic choices. current molecular and phenotypic methods to detect these organisms, however, require additional testing beyond routine organism identification. in this study, we evaluated the clinical performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) to detect pkpqil_p019 (p019)-an ∼11,109-da protein associated ... | 2015 | 26338858 |
| restriction profiling of 23s microheterogenic ribosomal repeats for detection and characterizing of e. coli and their clonal, pathogenic, and phylogroups. | correlating ribosomal microheterogenicity with unique restriction profiles can prove to be an efficacious and cost-effective approach compared with sequencing for microbial identification. an attempt to peruse restriction profiling of 23s ribosomal assemblage was ventured; digestion patterns with bfa i discriminated e. coli from its colony morphovars, while hae iii profiles assisted in establishing distinct clonal groups. among the gene pool of 399 ribosomal sequences extrapolated from 57 e. col ... | 2015 | 26885397 |
| associations between single nucleotide polymorphisms of high mobility group box 1 protein and clinical outcomes in korean sepsis patients. | high mobility group box 1 (hmgb1) plays a central role in the pathogenesis of sepsis and multiple organ dysfunction syndromes. we investigated the associations of a single nucleotide polymorphism (snp; rs1045411) in hmgb1 with various clinical parameters, severity, and prognosis in patients with sepsis, severe sepsis, or septic shock. | 2015 | 26632390 |
| associations between single nucleotide polymorphisms of high mobility group box 1 protein and clinical outcomes in korean sepsis patients. | high mobility group box 1 (hmgb1) plays a central role in the pathogenesis of sepsis and multiple organ dysfunction syndromes. we investigated the associations of a single nucleotide polymorphism (snp; rs1045411) in hmgb1 with various clinical parameters, severity, and prognosis in patients with sepsis, severe sepsis, or septic shock. | 2015 | 26632390 |
| in vitro activities of ceftazidime-avibactam, aztreonam-avibactam, and a panel of older and contemporary antimicrobial agents against carbapenemase-producing gram-negative bacilli. | among 177 carbapenemase-producing gram-negative bacilli (108 kpc, 32 ndm, 11 imp, 8 oxa-48, 4 oxa-181, 2 oxa-232, 5 imi, 4 vim, and 3 sme producers), aztreonam-avibactam was active against all isolates except two ndm producers with elevated mics of 8/4 and 16/4 mg/liter; ceftazidime-avibactam was active against all kpc-, imi-, sme-, and most oxa-48 group-producing isolates (93%) but not metallo-β-lactamase producers. among older and contemporary antimicrobials, the most active were colistin, tig ... | 2015 | 26392487 |
| community-acquired pneumonia and empyema caused by citrobacter koseri in an immunocompetent patient. | citrobacter species, belonging to the family enterobacteriaceae, are environmental organisms commonly found in soil, water, and the intestinal tracts of animals and humans. citrobacter koseri is known to be an uncommon but serious cause of both sporadic and epidemic septicemia and meningitis in neonates and young infants. most cases reported have occurred in immunocompromised hosts. the infections caused by citrobacter are difficult to treat with usual broad spectrum antibiotics owing to rapid g ... | 2015 | 26634165 |
| adverse events associated with fosfomycin use: review of the literature and analyses of the fda adverse event reporting system database. | the growing problem of antibacterial resistance resulted in an increased interest in fosfomycin, especially its parenteral formulation. we reviewed fosfomycin safety profile using the food and drug administration adverse event (ae) reporting system (faers) and published literature. | 2015 | 26437630 |
| bacteriological profile of surgical site infections and their antibiogram: a study from resource constrained rural setting of uttarakhand state, india. | surgical site infections (ssi) constitute a major public health problem worldwide and are the second most frequently reported nosocomial infections. they are responsible for increasing the treatment cost, length of hospital stay and significant morbidity and mortality. | 2015 | 26557520 |
| evaluation of the rapidec carba np test for detection of carbapenemases in enterobacteriaceae. | this study evaluated the performance of the rapidec carba np test, which was introduced recently into the market for the detection of carbapenemase production in a broad spectrum of β-lactamase-producing enterobacteriaceae clinical isolates. in total, 252 clinical enterobacteriaceae isolates that had been genetically characterized with respect to carbapenemase, extended-spectrum β-lactamase (esbl), and ampc genes were analyzed; 51/252 isolates (20.2%) were genetically confirmed to be carbapenema ... | 2015 | 26424840 |
| detection of carbapenemases in clinical enterobacteriaceae isolates using the vitek ast-n202 card. | the rapid and accurate detection of carbapenemase-producing enterobacteriaceae (cpe) in clinical microbiology laboratories is essential for the treatment and control of infections caused by these microorganisms. this study was performed to evaluate the ability of the vitek ast-n202 card to detect cpe isolates. | 2015 | 26483990 |
| mobile genetic elements related to the diffusion of plasmid-mediated ampc β-lactamases or carbapenemases from enterobacteriaceae: findings from a multicenter study in spain. | we examined the genetic context of 74 acquired ampc genes and 17 carbapenemase genes from 85 of 640 enterobacteriaceae isolates collected in 2009. using s1 pulsed-field gel electrophoresis and southern hybridization, 37 of 74 bla ampc genes were located on large plasmids of different sizes belonging to six incompatibility groups. we used sequencing and pcr mapping to investigate the regions flanking the acquired ampc genes. the bla cmy-2-like genes were associated with isecp1; the surrounding bl ... | 2015 | 26077249 |
| impact of sepsis classification and multidrug-resistance status on outcome among patients treated with appropriate therapy. | to assess the impact of sepsis classification and multidrug-resistance status on outcome in patients receiving appropriate initial antibiotic therapy. | 2015 | 25855900 |
| evaluation of two phenotypic screening tests for carbapenemase-producing enterobacteriaceae. | we evaluated the performance of two rapid tests for detection of carbapenemase-producing enterobacteriaceae (cpe) strains. the sensitivities and the specificities were 97.6% and 94.4% for the rapid carb screen and 98.8% and 93.1% for the kpc/mbl & oxa-48 confirm tests, providing the usefulness of these tools for screening cpe in microbiology wards. | 2015 | 26224841 |
| dominance of imp-4-producing enterobacter cloacae among carbapenemase-producing enterobacteriaceae in australia. | the prevalence of carbapenemase-producing enterobacteriaceae (cpe) has been increasing worldwide. blaimp has been reported to be the predominant carbapenemase-encoding gene within enterobacteriaceae in australia. however, there are limited data currently available on cpe from queensland, australia. a total of 58 cpe isolates were isolated between july 2009 and march 2014 from queensland hospitals. the clonality of isolates was determined by diversilab repetitive sequence-based pcr. the isolates ... | 2015 | 25918153 |
| comparison of sublingual therapeutic vaccine with antibiotics for the prophylaxis of recurrent urinary tract infections. | to compare the clinical impact of a prophylactic treatment with sublingual immunostimulation in the prevention of recurrent urinary tract infections (rutis) with the use of antibiotics. | 2015 | 26090341 |
| bacterial epidemiology and antimicrobial resistance in the surgery wards of a large teaching hospital in southern italy. | surgical infections represent an increasingly important problem for the national health system. in this study we retrospectively evaluated the bacterial epidemiology and antimicrobial susceptibility of the microorganisms concerned as well as the utilization of antibiotics in the general and emergency surgery wards of a large teaching hospital in southern italy in the period 2011-2013. | 2015 | 26075047 |
| identification and clinical significance of helcococcus kunzii in human samples. | from 2008 to 2013, 39 helcococcus kunzii strains were collected from human clinical specimens (79% from foot ulcers), and 85% of the 39 patients were infected. matrix-assisted laser desorption ionization-time of flight (maldi-tof) mass spectrometry and molecular methods accurately identified all isolates. this large study of clinical observations confirms the potential pathogenic role of h. kunzii. | 2015 | 25994161 |
| ceftazidime-avibactam activity tested against enterobacteriaceae isolates from u.s. hospitals (2011 to 2013) and characterization of β-lactamase-producing strains. | ceftazidime-avibactam (mic50/90, 0.12/0.25 μg/ml) inhibited 99.9% (20,698/20,709) of enterobacteriaceae isolates at ≤8 μg/ml. this compound was active against resistant subsets, including ceftazidime-nonsusceptible enterobacter cloacae (mic50/90, 0.25/0.5 μg/ml) and extended-spectrum β-lactamase (esbl) phenotype isolates. an esbl phenotype was noted among 12.4% (1,696/13,692 isolates from targeted species) of the isolates, including 776 escherichia coli (12.0% for this species; mic50/90, 0.12/0. ... | 2015 | 25845862 |
| efficient detection of carbapenemase activity in enterobacteriaceae by matrix-assisted laser desorption ionization-time of flight mass spectrometry in less than 30 minutes. | the recognition of carbapenemase-producing enterobacteriaceae (cpe) isolates is a major laboratory challenge, and their inappropriate or delayed detection may have negative impacts on patient management and on the implementation of infection control measures. we describe here a matrix-assisted laser desorption ionization-time of flight (maldi-tof)-based method to detect carbapenemase activity in enterobacteriaceae. after a 20-min incubation of the isolate with 0.5 mg/ml imipenem at 37°c, superna ... | 2015 | 25926485 |
| a comparison of the clinical characteristics of elderly and non-elderly women with community-onset, non-obstructive acute pyelonephritis. | acute pyelonephritis (apn) is the most common cause of community-onset bacteremia in hospitalized elderly patients. the objectives of this study were to investigate the differences in the clinical and microbiological data of hospitalized elderly and non-elderly women with community-onset apn. | 2015 | 25995668 |
| brain abscess caused by citrobacter koseri infection in an adult. | citrobacter koseri is a gram-negative bacillus that causes mostly meningitis and brain abscesses in neonates and infants. however, brain abscess caused by citrobacter koseri infection in an adult is extremely rare, and only 2 cases have been described. here, we reported a 73-year-old male presenting with a 3-week headache. a history of diabetes mellitus was noted. the images revealed a brain abscess in the left frontal lobe and pus culture confirmed the growth of citrobacter koseri. the clinical ... | 2015 | 25864072 |
| carbapenem-nonsusceptible enterobacteriaceae in taiwan. | a total of 1135 carbapenem-resistant (nonsusceptible) enterobacteriaceae (cre) isolates were recovered between november 2010 and july 2012 (517 from 2010-2011 and 618 from 2012) from 4 hospitals in taiwan. carbapenemase-producing enterobacteriaceae (cpe) comprised 5.0% (57 isolates), including 17 kpc-2 (16 klebsiella pneumoniae and 1 escherichia coli), 1 ndm-1 (k. oxytoca), 37 imp-8 (26 enterobacter cloacae, 4 citrobacter freundii, 4 raoultella planticola, 1 k. pneumoniae, 1 e. coli and 1 k. oxy ... | 2015 | 25794144 |
| enterobacteriaceae isolates from the oral cavity of workers in a brazilian oncology hospital. | the evaluation of workers as potential reservoirs and disseminators of pathogenic bacteria has been described as a strategy for the prevention and control of healthcare-associated infections (hais). the aim of this study was to evaluate the presence of enterobacteriaceae in the oral cavity of workers at an oncology hospital in the midwest region of brazil, as well as to characterize the phenotypic profile of the isolates. saliva samples of 294 workers from the hospital's healthcare and support t ... | 2015 | 25923890 |
| bacteriology of pressure ulcers in individuals with spinal cord injury: what we know and what we should know. | individuals with spinal cord injury (sci) are at increased risk for the development of pressure ulcers. these chronic wounds are debilitating and contribute to prolonged hospitalization and worse medical outcome. however, the species of bacteria and the role that specific species may play in delaying the healing of chronic pressure ulcers in the sci population has not been well characterized. this study will review the literature regarding what is known currently about the bacteriology of pressu ... | 2015 | 25130374 |
| rapid detection of esbl-producing enterobacteriaceae in blood cultures. | we rapidly identified extended-spectrum β-lactamase (esbl) producers prospectively among 245 gram-negative bacilli-positive cultured blood specimens using the rapid esbl nordmann/dortet/poirel test and direct bacterial identification using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. this combination identified esbl-producing enterobacteriaceae within 30 min and had high predictive values. | 2015 | 25695535 |
| novel approaches to improve the intrinsic microbiological safety of powdered infant milk formula. | human milk is recognised as the best form of nutrition for infants. however; in instances where breast-feeding is not possible, unsuitable or inadequate, infant milk formulae are used as breast milk substitutes. these formulae are designed to provide infants with optimum nutrition for normal growth and development and are available in either powdered or liquid forms. powdered infant formula is widely used for convenience and economic reasons. however; current manufacturing processes are not capa ... | 2015 | 25685987 |
| association of tellurite resistance with hypervirulent clonal groups of klebsiella pneumoniae. | tellurite-based selective growth media are used for several bacterial pathogens. we found that, in klebsiella pneumoniae, tellurite resistance is strongly associated with hypervirulent clonal group 23 (cg23), cg65, and cg86, providing a novel approach for screening environmental or carriage samples. the terw gene was also associated with these groups. | 2015 | 25631812 |
| blocking yersiniabactin import attenuates extraintestinal pathogenic escherichia coli in cystitis and pyelonephritis and represents a novel target to prevent urinary tract infection. | the emergence and spread of extended-spectrum beta-lactamases and carbapenemases among common bacterial pathogens are threatening our ability to treat routine hospital- and community-acquired infections. with the pipeline for new antibiotics virtually empty, there is an urgent need to develop novel therapeutics. bacteria require iron to establish infection, and specialized pathogen-associated iron acquisition systems like yersiniabactin, common among pathogenic species in the family enterobacter ... | 2015 | 25624354 |
| prevalence and antimicrobial resistance of microbes causing bloodstream infections in unguja, zanzibar. | bloodstream infections (bsi) are frequent and cause high case-fatality rates. urgent antibiotic treatment can save patients' lives, but antibiotic resistance can render antibiotic therapy futile. this study is the first to collect epidemiological data on bsi from unguja, zanzibar. | 2015 | 26700032 |
| purification, characterization of l-methioninase from candida tropicalis, and its application as an anticancer. | the aim of the present study is to purify l-methioninase from candida tropicalis 34.19-fold with 27.98% recovery after ion exchange chromatography followed by gel filtration. the purified enzyme revealed a single band on sds-page gel with a molecular weight of 46 kda. its optimum temperature was 45 to 55 and thermal stability was 55°c for 15 min. the enzyme had optimum ph at 6.5 and stability at a ph range of 5.5 to 7.0 for 24 hr. the maximum activity was observed with substrate concentration of ... | 2015 | 26691554 |
| the role of plant-microbe interactions and their exploitation for phytoremediation of air pollutants. | since air pollution has been linked to a plethora of human health problems, strategies to improve air quality are indispensable. despite the complexity in composition of air pollution, phytoremediation was shown to be effective in cleaning air. plants are known to scavenge significant amounts of air pollutants on their aboveground plant parts. leaf fall and runoff lead to transfer of (part of) the adsorbed pollutants to the soil and rhizosphere below. after uptake in the roots and leaves, plants ... | 2015 | 26516837 |
| immune-relevant and antioxidant activities of vitellogenin and yolk proteins in fish. | vitellogenin (vtg), the major egg yolk precursor protein, is traditionally thought to provide protein- and lipid-rich nutrients for developing embryos and larvae. however, the roles of vtg as well as its derived yolk proteins lipovitellin (lv) and phosvitin (pv) extend beyond nutritional functions. accumulating data have demonstrated that vtg, lv and pv participate in host innate immune defense with multifaceted functions. they can all act as multivalent pattern recognition receptors capable of ... | 2015 | 26506386 |
| resistance integrons: class 1, 2 and 3 integrons. | as recently indiscriminate abuse of existing antibiotics in both clinical and veterinary treatment leads to proliferation of antibiotic resistance in microbes and poses a dilemma for the future treatment of such bacterial infection, antimicrobial resistance has been considered to be one of the currently leading concerns in global public health, and reported to widely spread and extended to a large variety of microorganisms. in china, as one of the currently worst areas for antibiotics abuse, the ... | 2015 | 26487554 |
| deterioration to extinction of wastewater bacteria by non-thermal atmospheric pressure air plasma as assessed by 16s rdna-dgge fingerprinting. | the use of cold plasma jets for inactivation of a variety of microorganisms has recently been evaluated via culture-based methods. accordingly, elucidation of the role of cold plasma in decontamination would be inaccurate because most microbial populations within a system remain unexplored owing to the high amount of yet uncultured bacteria. the impact of cold atmospheric plasma on the bacterial community structure of wastewater from two different industries was investigated by metagenomic-based ... | 2015 | 26500637 |
| sulfoxides, analogues of l-methionine and l-cysteine as pro-drugs against gram-positive and gram-negative bacteria. | the problem of resistance to antibiotics requires the development of new classes of broad-spectrum antimicrobial drugs. the concept of pro-drugs allows researchers to look for new approaches to obtain effective drugs with improved pharmacokinetic and pharmacodynamic properties. thiosulfinates, formed enzymatically from amino acid sulfoxides upon crushing cells of genus allium plants, are known as antimicrobial compounds. the instability and high reactivity of thiosulfinates complicate their use ... | 2015 | 26798500 |
| dissemination of carbapenemases producing gram negative bacteria in the middle east. | the emergence and spread of carbapenemase-producing bacteria, that hydolyze most β-lactams, including carbapenems, are a major concern of public health system worldwide, particularly in the middle east area. since the plasmids harboring resistance genes could be spread across other bacterial populations, detection of carbapenemase-producing organisms has become more problematic. these organisms produce different types of enzymes including the most prevalent types including kpc, vim, imp, ndm, an ... | 2015 | 26719779 |
| epidemiology and characteristics of metallo-β-lactamase-producing pseudomonas aeruginosa. | metallo-β-lactamase-producing pseudomonas aeruginosa (mppa) is an important nosocomial pathogen that shows resistance to all β-lactam antibiotics except monobactams. there are various types of metallo-β-lactamases (mbls) in carbapenem-resistant p. aeruginosa including imipenemase (imp), verona integron-encoded metallo-β-lactamase (vim), sao paulo metallo-β-lactamase (spm), germany imipenemase (gim), new delhi metallo-β-lactamase (ndm), florence imipenemase (fim). each mbl gene is located on spec ... | 2015 | 26157586 |
| psychrotrophic bacteria in milk: how much do we really know? | the occurrence of psychrotrophic bacteria in raw milk is studied worldwide due to the difficulties associated with controlling their growth during cold storage and the consequent negative effects upon fluid milk or dairy products. among the psychrotrophic bacteria, the genus pseudomonas (represented primarily by p. fluorescens) has been highlighted as the cause of numerous defects in dairy products. in light of its perceived predominance, this species has frequently been chosen as a model organi ... | 2015 | 26273245 |
| conjunctival flora of clinically normal and diseased turtles and tortoises. | in captive breed turtles and tortoises conjunctival disease is common. our aim was to investigate the bacterial and fungal flora present in the eyes of healthy and pathological chelonians and to compare findings in turtles with those in tortoises. | 2015 | 25889261 |