Publications

differences in the occurrence and epidemiology of cryptosporidiosis in aboriginal and non-aboriginal people in western australia (2002-2012).	cryptosporidiosis is a diarrhoeal illness caused by the protozoan parasite cryptosporidium. in australia, very little is known about the epidemiology of cryptosporidiosis in aboriginal peoples. the present study analysed long-term cryptosporidiosis patterns across western australia (wa) (2001-2012), combined with genotyping and subtyping data at the 18s and glycoprotein 60 (gp60) loci respectively. comparison of cryptosporidiosis notifications between aboriginal and non-aboriginal people in wa, ...	2017	28536073
occurrence of cryptosporidium and giardia and the relationship between protozoa and water quality indicators in swimming pools.	a total of 60 samples were collected from 35 swimming pools in beijing, china, and the presence of cryptosporidium and giardia were investigated. the results showed that 16.7% and 15.0% of samples were positive for cryptosporidium oocyst and giardia cysts, respectively, with a mean concentration of 0.30 oocysts/10 l and 0.27 cysts/10 l. the oocysts and cysts were found to have higher rates of occurrence in august than in may. genotyping confirmed the presence of cryptosporidium hominis, c. parvu ...	2017	28506034
detection of cryptosporidium in stool samples of immunocompromised patients.	cryptosporidium species is the most common opportunistic enteric parasite encountered in the immunocompromised patients. considering the need to diagnose them early relies mostly on rapid tests such as antigen detection by immunochromatographic test (ict), elisa, and microscopy. however, the sensitivity and specificity varies with different methods and different kits used. this study was conducted to determine the intestinal parasitic profile in immunocompromised patients and to assess the diagn ...	2017	28459014
coccidian intestinal parasites among immunocompetent children presenting with diarrhea: are we missing them?	diarrhoea is an important cause of both morbidity and mortality among children in india. coccidian parasitic infections are an important cause of diarrhea in immunocompromised patients, but their investigations are rarely sought by the treating physicians in seemingly immunocompetent children. this study was aimed to find the incidence rate of coccidian parasites in all children presented with diarrhoea, irrespective of their immune status.	2017	28459013
rodents as a reservoir of infection caused by multiple zoonotic species/genotypes of c. parvum, c. hominis, c. suis, c. scrofarum, and the first evidence of c. muskrat genotypes i and ii of rodents in europe.	cryptosporidium spp. is an important causative agent of intestinal parasitoses-induced diarrhoea in humans and animals worldwide. rodents (small mammals), the main reservoir of infections, are globally expanded and overpopulated, which increases the risk of transfer of human and zoonotic pathogens from the genus cryptosporidium. in this study, cryptosporidium was detected in wild immunocompetent asymptomatic small mammals. altogether 262 fecal samples were collected from five areas in eastern sl ...	2017	28433573
early outbreak detection by linking health advice line calls to water distribution areas retrospectively demonstrated in a large waterborne outbreak of cryptosporidiosis in sweden.	in the winter and spring of 2011 a large outbreak of cryptosporidiosis occurred in skellefteå municipality, sweden. this study summarizes the outbreak investigation in terms of outbreak size, duration, clinical characteristics, possible source(s) and the potential for earlier detection using calls to a health advice line.	2017	28420373
multiplex pcr detection of cryptosporidium sp, giardia lamblia and entamoeba histolytica directly from dried stool samples from guinea-bissauan children with diarrhoea.	in developing countries, diarrhoea is the most common cause of death for children under five years of age, with giardia lamblia, cryptosporidium and entamoeba histolytica as the most frequent pathogenic parasites. traditional microscopy for stool parasites has poor sensitivity and specificity, while new molecular methods may provide more accurate diagnostics. in poor regions with sample storage hampered by uncertain electricity supply, research would benefit from a method capable of analysing dr ...	2017	28446068
in silico identification and validation of a novel hypothetical protein in cryptosporidium hominis and virtual screening of inhibitors as therapeutics.	computational approaches to predict structure/function and other biological characteristics of proteins are becoming more common in comparison to the traditional methods in drug discovery. cryptosporidiosis is a major zoonotic diarrheal disease particularly in children, which is caused primarily by cryptosporidium hominis and cryptosporidium parvum. currently, there are no vaccines for cryptosporidiosis and recommended drugs are ineffective. with the availability of complete genome sequence of c ...	2017	28389892
understanding the molecular mechanism of substrate channeling and domain communication in protozoal bifunctional ts-dhfr.	most species, such as humans, have monofunctional forms of thymidylate synthase (ts) and dihydrofolate reductase (dhfr) that are key folate metabolism enzymes making critical folate components required for dna synthesis. in contrast, several parasitic protozoa, including leishmania majorg(lm), plasmodium falciparum (pf), toxoplasma gondii (tg) and cryptosporidium hominis(ch), contain a unique bifunctional thymidylate synthase-dihydrofolate reductase (ts-dhfr) having the two sequential catalytic ...	2017	28338744
cryptosporidium: identification and genetic typing.	cryptosporidium spp. are obligate protozoan parasites of the gastrointestinal tract of vertebrates, including humans. in the majority of human cases, the diarrheal disease cryptosporidiosis is caused by either the human-adapted species cryptosporidium hominis or the zoonotic cryptosporidium parvum 'bovine genotype' (also known as cryptosporidium pestis). the infectious stage, environmentally resilient cryptosporidium oocysts, are shed by the infected host. cryptosporidium parasites are transmitt ...	2017	28166384
first description of cryptosporidium hominis gp60 genotype ika20g1 and cryptosporidium parvum gp60 genotypes iiaa18g3r1 and iiaa15g2r1 in foals in brazil.	the present study focuses on cryptosporidium infections of foals in brazil. a total of 92 animals of different breeds from 11 farms in the vicinity of araçatuba in the state of são paulo, were examined. according to pcr targeting the 18s rrna gene, cryptosporidium sp. dna was detected in 21.7% (20/92) of foals. good quality 18s rrna, actin, hsp70 and gp60 genes npcr amplicons were obtained from five fecal samples. pcr amplification and sequencing of a fragment of the gp60 sporozoite surface glyc ...	2017	28043388
natural history of cryptosporidiosis in a birth cohort in southern india.	cryptosporidium is a leading cause of moderate to severe childhood diarrhea in resource-poor settings. understanding the natural history of cryptosporidiosis and the correlates of protection are essential to develop effective and sustainable approaches to disease control and prevention.	2017	28013266
genomic variation in iba10g2 and other patient-derived cryptosporidium hominis subtypes.	in order to improve genotyping and epidemiological analysis of cryptosporidium spp., genomic data need to be generated directly from a broad range of clinical specimens. utilizing a robust method that we developed for the purification and generation of amplified target dna, we present its application for the successful isolation and whole-genome sequencing of 14 different cryptosporidium hominis patient specimens. six isolates of subtype iba10g2 were analyzed together with a single representativ ...	2017	28003424
real-time nucleic acid sequence-based amplification (nasba) assay targeting mic1 for detection of cryptosporidium parvum and cryptosporidium hominis oocysts.	both cryptosporidium parvum and cryptosporidium hominis are often associated with cryptosporidiosis in humans, but whereas humans are the main host for c. hominis, c. parvum is zoonotic and able to infect a variety of species. the oocyst transmission stages of both species of parasites are morphologically identical and molecular techniques, usually polymerase chain reaction (pcr), are required to distinguish between oocysts detected by standard methods in environmental samples, such as water. in ...	2017	27998735
occurrence and genetic characteristics of cryptosporidium hominis and cryptosporidium andersoni in horses from southwestern china.	a total of 333 fecal specimens from horses in southwestern china were genotyped based on analysis of the small subunit rrna (ssu rrna) gene. cryptosporidium hominis and cryptosporidium andersoni were identified in 2 and 4 stool specimens, respectively. the identification of c. hominis was confirmed by sequence analysis of the 70-kda heat shock protein (hsp70) and oocyst wall protein (cowp) genes. subtyping analysis of the 60-kda glycoprotein (gp60) gene sequence of c. hominis revealed a new rare ...	2017	28166378