Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| in-vitro antifungal susceptibility of clinical and environmental fusarium spp. strains. | the mics of amphotericin b, miconazole, ketoconazole, flucytosine, itraconazole and fluconazole for 19 isolates of fusarium oxysporum, 16 fusarium solani, seven fusarium verticilliodes, four fusarium proliferatum, four fusarium dimerum, three fusarium equiseti, and one each of the following species: fusarium graminearum, fusarium chlamydosporum, fusarium semitectum, fusarium avenaceum and fusarium subglutinans were determined by a broth microdilution method. thirty-eight of these isolates were o ... | 1997 | 9069536 |
| cloning of cutinase transcription factor 1, a transactivating protein containing cys6zn2 binuclear cluster dna-binding motif. | hydroxy fatty acids from plant cutin were shown previously to induce the expression of the cutinase gene via a palindromic sequence located at -159 base pairs of the cutinase gene in fusarium solani f. sp. pisi (nectria hematococca mating type vi). of the two overlapping palindromes in this sequence, palindrome 2 was found to be essential for the inducibility of cutinase by hydroxy fatty acids. screening of a phage expression library with the concatenated palindrome 2 as probe detected a distinc ... | 1997 | 9139694 |
| invasive fusarium infections: a retrospective survey of 31 cases. the french 'groupe d'etudes des mycoses opportunistes' gemo. | a retrospective study was conducted in france to investigate fusarium infections which are now recognized as emerging opportunistic infections. the clinical and mycological findings for 31 cases diagnosed between 1984 and 1993 by members of the french groupe d'etudes des mycoses opportunistes were analysed. all suffered from haematological disease, most often acute leucaemia (n = 19). twenty-two had received cytostatic chemotherapy and ten had undergone bone marrow transplantation. prolonged apl ... | 1997 | 9147270 |
| atomic resolution (1.0 a) crystal structure of fusarium solani cutinase: stereochemical analysis. | x-ray data have been recorded to 1.0 a resolution from a crystal of fusarium solani cutinase using synchrotron radiation and an imaging-plate scanner. the anisotropic treatment of thermal motion led to a fivefold increase in accuracy and to a considerable quality improvement in the electron density maps with respect to an intermediate isotropic model. the final model has an r-factor of 9.4%, with a mean coordinate error of 0.021 a, as estimated from inversion of the least-squares matrix. the ava ... | 1997 | 9175860 |
| polyhexamethylene biguanide (phmb) in the treatment of experimental fusarium keratomycosis. | we wanted to determine whether topical polyhexamethylene biguanide (phmb) 0.02% was effective in the treatment of experimental fusarium keratomycosis in rabbits. | 1997 | 9220243 |
| fungaemia due to fusarium spp. in cancer patients. | five cases of fungaemia due to fusarium spp. in cancer patients are described. two were breakthrough cases, despite ongoing therapy with amphotericin b. three were caused by fusarium solani, one by f. oxysporum and one by f. dimerum. four patients died, three of them despite therapy with amphotericin b for between 5-37 days. we describe only the second reported case of f. dimerum fungaemia. since 1972, 93 cases of systemic infection with fusarium spp. have been described: 43 had positive blood c ... | 1997 | 9253703 |
| primary pulmonary involvement of fusarium solani in a lung transplant recipient. | a case of cavitary lung disease caused by fusarium solani in a lung transplant recipient is presented. a mechanism for development of this infection is proposed. lipid complex amphotericin b (abelcet) was effective in eradicating this infection. to our knowledge, invasive lung disease caused by the fusarium species has not been previously reported in a solid organ transplant recipient. | 1997 | 9377931 |
| 1h, 13c, and 15n resonance assignments of fusarium solani pisi cutinase and preliminary features of the structure in solution. | essentially complete (96%) sequence-specific assignments were made for the backbone and side-chain 1h, 13c, and 15n resonances of fusarium solani pisi cutinase, produced as a 214-residue heterologous protein in escherichia coli, using heteronuclear nmr techniques. three structural features were noticed during the assignment. (1) the secondary structure in solution corresponds mostly with the structure from x-ray diffraction, suggesting that both structures are globally similar. (2) the hn of ala ... | 1997 | 9385640 |
| pectate lyase peli of erwinia chrysanthemi 3937 belongs to a new family. | erwinia chrysanthemi 3937 secretes five major isoenzymes of pectate lyases encoded by the pel4, pelb, pelc, peld, and pele genes and a set of secondary pectate lyases, two of which, pell and pelz, have been already identified. we cloned the peli gene, encoding a ninth pectate lyase of e. chrysanthemi 3937. the peli reading frame is 1,035 bases long, corresponding to a protein of 344 amino acids including a typical amino-terminal signal sequence of 19 amino acids. the purified mature peli protein ... | 1997 | 9393696 |
| cutaneous hyalohyphomycosis caused by fusarium solani in a loggerhead sea turtle (caretta caretta l.). | fusarium solani was reported as the agent of a cutaneous infection in an injured sea turtle collected in the mediterranean sea. the turtle was treated with both a topical 10% solution of iodine in alcohol and ketoconazole. the source of the causal agent was traced to the sand in the tank in which the turtle was maintained. the strain was only sensitive in vitro to amphotericin b and was resistant to 5-fluorocytosine, fluconazole, itraconazole, and ketoconazole. | 1997 | 9399554 |
| treatment of fungal corneal ulcers with amphotericin b ointment. | to report two patients with severe fungal corneal ulcers who were treated successfully with topical amphotericin b ointment. | 1997 | 9402830 |
| effect of gamma radiation on the survival of fungal and actinomycetal florae contaminating medicinal plants. | this study evaluates the effect of gamma radiation on the viability of fungi and actinomycetes that contaminate medicinal plants. the relationship between the total lipids of some fungi and actinomycetes and their sensitivity to gamma radiation is also investigated. the date reveal that the viable counts of these florae decrease approximately exponentially with the radiation dose, the effective dose for the elimination of these microorganisms being about 5 kgy for all the medicinal plants under ... | 1997 | 9022214 |
| allergic fungal sinusitis in the southeastern usa: involvement of a new agent epicoccum nigrum ehrenb. ex schlecht. 1824. | alternaria alternata, aspergillus spp., bipolaris spicifera, curvularia lunata, epicoccum nigrum and fusarium solani were isolated repeatedly from groups of patients among 96 diagnosed with allergic fungal sinusitis (afs). epicoccum nigrum was obtained consistently from four patients, one of whom yielded mycelial masses consistent in morphology with e. nigrum. fifteen of the predominant fungi recovered from air samples from selected patients' residences included the same species isolated from th ... | 1997 | 9467107 |
| crystal structure of cutinase covalently inhibited by a triglyceride analogue. | cutinase from fusarium solani is a lipolytic enzyme that hydrolyses triglycerides efficiently. all the inhibited forms of lipolytic enzymes described so far are based on the use of small organophosphate and organophosphonate inhibitors, which bear little resemblance to a natural triglyceride substrate. in this article we describe the crystal structure of cutinase covalently inhibited by (r)-1,2-dibutyl-carbamoylglycero-3-o-p-nitrophenylbutyl-phos phonate, a triglyceride analogue mimicking the fi ... | 1997 | 9041628 |
| fusarium solani fungemia in a bone marrow transplant recipient. | disseminated fusarium solani infection, with its distinctive skin lesions, is an emerging cause of mortality in bone marrow transplant recipients worldwide. however, it has never been reported before in taiwan. we report a 21-year-old man with disseminated fusariosis who developed fever, myalgia and generalized erythematous papules on day 8 after undergoing allogeneic bone marrow transplantation for severe aplastic anemia. histopathology of the skin lesion revealed mycotic emboli. cultures of bo ... | 1997 | 9071840 |
| effect of disruption of a cutinase gene (cuta) on virulence and tissue specificity of fusarium solani f. sp. cucurbitae race 2 toward cucurbita maxima and c. moschata. | a 3.9-kb genomic dna fragment from the cucurbit pathogen fusarium solani f. sp. cucurbitae race 2 was cloned. sequence analysis revealed an open reading frame of 690 nucleotides interrupted by a single 51-bp intron. the nucleotide and predicted amino acid sequences showed 92 and 98% identity, respectively, to those of the cuta gene of the pea pathogen f. solani f. sp. pisi. a gene replacement vector was constructed and used to generate cuta- mutants that were detected with a polymerase chain rea ... | 1997 | 9100380 |
| in vitro antifungal activity of pneumocandin l-743,872 against a variety of clinically important molds. | the in vitro activity of the new antifungal drug pneumocandin l-743,872 against 55 isolates of clinically important molds was examined by an adapted macrobroth dilution method for yeasts. pneumocandin l-743,872 exhibited in vitro antifungal activity against alternaria sp., aspergillus flavus, aspergillus fumigatus, curvularia lunata, exophiala jeanselmei, fonsecaea pedrosoi, paecilomyces variotii, and scedosporium apiospermum. the drug appeared to lack significant in vitro inhibitory activity ag ... | 1997 | 9257774 |
| immunohistologic identification of aspergillus spp. and other hyaline fungi by using polyclonal fluorescent antibodies. | isolation and identification of pathogenic aspergillus and fusarium spp. from clinical materials provide the most accurate means for establishing a diagnosis of infections by these molds. such efforts, however, are not always successful. histologic diagnosis also has its limitations. in vivo the hyphae of aspergillus and fusarium spp. are very similar and their in situ manifestations are not pathognomonic. to improve the histologic diagnosis of infections by aspergillus and fusarium species, we ... | 1997 | 9276388 |
| [resolution of a disseminated infection by fusarium solani in a patient with acute promyelocytic leukemia and granulocytopenia secondary to chemotherapy by the combined use of g-csf and liposomal amphotericin b]. | 1997 | 9289532 | |
| cloning and expression of cdna encoding a mitogen-activated protein kinase from a phytopathogenic filamentous fungus. | we have cloned a mitogen-activated protein kinase (mapk) designated fusarium solani f. sp. pisi mitogen-activated protein kinase (fsmapk) from the phytopathogenic filamentous fungus f. solani f. sp. pisi t8 strain. a single open reading frame (orf) of 1068 bp encoding a polypeptide of 355 amino acids (aa) with a predicted molecular mass of 41,194 da was found in the cloned 1583-bp cdna insert. fsmapk is highly homologous to spk1 of fission yeast, fus3 of budding yeast, mserk1 of alfalfa, sur-1 o ... | 1997 | 9305760 |
| scanning slit confocal microscopy of fungal keratitis. | in vivo scanning slit confocal microscopy was performed in a patient with fusarium solani keratitis. morphologically distinctive abundant filamentous structures were observed intrastromally. confocal microscopy of the culture plate growing f solani from the patient's corneal scraping revealed filaments morphologically similar to the filaments observed in vivo. after 1 week of medical therapy, subsequent confocal microscopy showed an increased load of filaments, supporting the decision to perform ... | 1997 | 9366681 |
| fusarium solani infection in a patient with acute myelogenous leukemia--a case report. | multiple necrotizing skin lesions due to fusarium solani in an elderly man with acute myelogenous leukemia is described. | 1997 | 9444872 |
| cyanide degradation under alkaline conditions by a strain of fusarium solani isolated from contaminated soils. | several cyanide-tolerant microorganisms have been selected from alkaline wastes and soils contaminated with cyanide. among them, a fungus identified as fusarium solani ihem 8026 shows a good potential for cyanide biodegradation under alkaline conditions (ph 9.2 to 10.7). results of k(sup14)cn biodegradation studies show that fungal metabolism seems to proceed by a two-step hydrolytic mechanism: (i) the first reaction involves the conversion of cyanide to formamide by a cyanide-hydrolyzing enzyme ... | 1997 | 16535647 |
| molecular cloning of tvdao1, a gene encoding a d-amino acid oxidase from trigonopsis variabilis and its expression in saccharomyces cerevisiae and kluyveromyces lactis. | the dao1 gene of trigonopsis variabilis encoding a d-amino acid oxidase (ec 1.4.3.3) was isolated from genomic clones selected for their specific hybridization to synthetic oligodeoxyribonucleotide probes based on regions of the enzyme that have been conserved through evolution. the nucleotide sequence of the gene predicts a protein with similarities to human, pig, rabbit, mouse and fusarium solani d-amino acid oxidases. the open reading frame of the t. variabilis dao1 gene was interrupted by an ... | 1997 | 9434346 |
| protein engineering the surface of enzymes. | the protein surface is the interface through which a protein senses the external world. its composition of charged, polar and hydrophobic residues is crucial for the stability and activity of the protein. the charge state of seven of the twenty naturally occurring amino acids is ph dependent. a total of 95% of all titratable residues are located on the surface of soluble proteins. in evolutionary related families of proteins such residues are particularly prone to substitutions, insertions and d ... | 1998 | 9866858 |
| bacterial-fungal biofilms in flowing water photo-processing tanks. | a complex seven species model community, including bacteria and fungi, was selected from organisms isolated from the walls of an industrial flowing water system. growth rates of the species were determined in single and mixed batch culture growth. the rates were found to be significantly higher in mixed culture for pseudomonas alcaligenes and flavobacterium indologenes and higher in single culture for xanthomonas maltophilia, rhodotorula glutinis and fusarium solani, whereas no significant diffe ... | 1998 | 9633659 |
| cutaneous fungal infection caused by fusarium solani. | 1998 | 11189218 | |
| structural characterization of neutral glycosphingolipids from fusarium species. | glycosphingolipids were extracted from hyphae of fusarium solani and from an unnamed fusarium species, and were purified by silica and iatrobead column chromatography. their structures were determined by compositional analysis, nuclear magnetic resonance spectroscopy, gas chromatography/mass spectrometry and by fast atom bombardment mass spectrometry of the native and peracetylated materials, which defined their sugar, long-chain base and fatty acid compositions. the locations of the double bond ... | 1998 | 9507119 |
| polymerase chain reaction and restriction fragment length polymorphism mediated detection and speciation of candida spp causing intraocular infection. | to determine the usefulness of polymerase chain reaction (pcr) and restriction fragment length polymorphism (rflp) analysis in the identification and speciation of candida spp that causes ocular infection. | 1998 | 9579465 |
| two-dimensional nmr experiments for the assignment of aromatic side chains in 13c-labeled proteins | as aromatic residues very often are part of the hydrophobic core of proteins, the unambiguous assignment of the aromatic proton resonances is essential for an accurate and precise structure determination. instead of transferring 1hbeta coherence to the aromatic protons via 13cgamma like in a number of published methods, in our new experiments the 13cgamma resonances are first correlated with the 1hbeta chemical shifts in one experiment and then with the aromatic proton resonances in four other e ... | 1998 | 9469899 |
| exploring hydrophobic sites in proteins with xenon or krypton. | x-ray diffraction is used to study the binding of xenon and krypton to a variety of crystallised proteins: porcine pancreatic elastase; subtilisin carlsberg from bacillus licheniformis; cutinase from fusarium solani; collagenase from hypoderma lineatum; hen egg lysozyme, the lipoamide dehydrogenase domain from the outer membrane protein p64k from neisseria meningitidis; urate-oxidase from aspergillus flavus, mosquitocidal delta-endotoxin cytb from bacillus thuringiensis and the ligand-binding do ... | 1998 | 9443341 |
| detection of fungemia from blood cultures using the bactec 9240 instrument. | from january 1995 to november 1996, 14,623 resin-containing blood culture vials were tested with the bactec fluorescent series instrument. a total of 1560 microorganisms were recovered. 48 of the microorganisms were fungi. we could demonstrate the ability of the bactec 9240 to detect cases of fungemia with candida species and moulds such as aspergillus fumigatus (4 cases) and fusarium solani (1 case). | 1998 | 9532264 |
| diagnosis of fusarium keratitis in an animal model using the polymerase chain reaction. | the purpose of this study was apply the polymerase chain reaction (pcr) to develop a sensitive, specific, and rapid test to diagnose fusarium keratitis. fusarium is the most common cause of fungal corneal infection in some parts of the world. it is often difficult to establish that a keratitis is due to fungal infection. | 1998 | 9602631 |
| structure-in vitro activity relationships of pentamidine analogues and dication-substituted bis-benzimidazoles as new antifungal agents. | twenty analogues of pentamidine, 7 primary metabolites of pentamidine, and 30 dicationic substituted bis-benzimidazoles were screened for their inhibitory and fungicidal activities against candida albicans and cryptococcus neoformans. a majority of the compounds had mics at which 80% of the strains were inhibited (mic80s) comparable to those of amphotericin b and fluconazole. unlike fluconazole, many of these compounds were found to have potent fungicidal activity. the most potent compound again ... | 1998 | 9756747 |
| in vitro antifungal activities of a series of dication-substituted carbazoles, furans, and benzimidazoles. | aromatic dicationic compounds possess antimicrobial activity against a wide range of eucaryotic pathogens, and in the present study an examination of the structures-functions of a series of compounds against fungi was performed. sixty-seven dicationic molecules were screened for their inhibitory and fungicidal activities against candida albicans and cryptococcus neoformans. the mics of a large number of compounds were comparable to those of the standard antifungal drugs amphotericin b and flucon ... | 1998 | 9756748 |
| cutinase binding and activity at the triolein-water interface monitored by oil drop tensiometry. | changes of the oil-water interfacial tension resulting from binding of fusarium solani pisi cutinase and subsequent lipid hydrolysis were investigated using the oil drop technique. an elisa was developed to determine the amount of cutinase bound to the triolein-water interface after biotinylation of the enzyme. cutinase irreversibly adsorbs to a maximum value of about 2 mg/m2. a minimal specific activity of 110 mumol/min/mg was calculated for cutinase acting on a single oil droplet, which is clo ... | 1998 | 9853365 |
| identification of functional and unfolding motions of cutinase as obtained from molecular dynamics computer simulations. | the implementation of cutinase from fusarium solani pisi as a fat-stain removing ingredient in laundry washing is hampered by its unfolding in the presence of anionic surfactants. in this work we present molecular dynamics (md) computer simulations on cutinase and analysis procedures to distinguish the movements related to its functional behavior (e.g., substrate binding) from those related to the unfolding of the enzyme. two kinds of md-simulations were performed: a simulation mimicking the the ... | 1998 | 9779792 |
| [fusarium solani infection in a hematology unit. study of 2 cases and 2 isolations]. | 1998 | 9810555 | |
| soil fungi of some low-altitude desert cotton fields and ability of their extracts to inhibit aspergillus flavus. | soil is presumed to be a major source of inoculum for aspergillus flavus which contaminates cottonseed and produces the potent carcinogen, aflatoxin. little is known about the mycoflora of the low desert soils of cotton fields where aflatoxin is a chronic problem. in this study, soils from cotton fields in southwestern arizona and south-eastern california were assayed for filamentous fungi. forty-two taxa, predominantly in the genera aspergillus, penicillium and fusarium, were isolated. to deter ... | 1998 | 9926421 |
| rapid extraction of genomic dna from medically important yeasts and filamentous fungi by high-speed cell disruption. | current methods of dna extraction from different fungal pathogens are often time-consuming and require the use of toxic chemicals. dna isolation from some fungal organisms is difficult due to cell walls or capsules that are not readily susceptible to lysis. we therefore investigated a new and rapid dna isolation method using high-speed cell disruption (hscd) incorporating chaotropic reagents and lysing matrices in comparison to standard phenol-chloroform (pc) extraction protocols for isolation o ... | 1998 | 9620390 |
| disseminated fusarium solani infection with endocarditis in a lung transplant recipient. | eleven days after double lung transplantation for cystic fibrosis, an 18-year-old patient developed a disseminated fusarium solani infection with tricuspid valve endocarditis. this infection occurred under fluconazole and immunosuppressive therapy with cyclosporin, prednisone and azathioprine, with a normal leucocyte count. liposomal amphotericin b allowed blood culture negativation. the patient died from a bacterial septic shock. | 1998 | 9610136 |
| keratin degradation by scytalidium species and fusarium solani. | the keratin degradation capability of some non-dermatophytic filamentous fungi, viz scytalidium dimidiatum types a and b, s. hyalinum, s. japonicum and fusarium solani, was investigated using nail clippings and keratin powder as substrates. representative isolates of the dermatophytes trichophyton rubrum and t. mentagrophytes were also tested. the two morphological types of s. dimidiatum were almost equally effective in the degradation of substrates, 42-43% nail clippings and 24-26% keratin. scy ... | 1998 | 9610139 |
| packing forces in nine crystal forms of cutinase. | during the characterization of mutants and covalently inhibited complexes of fusarium solani cutinase, nine different crystal forms have been obtained so far. protein mutants with a different surface charge distribution form new intermolecular salt bridges or long-range electrostatic interactions that are accompanied by a change in the crystal packing. the whole protein surface is involved in the packing contacts and the hydrophobicities of the protein surfaces in mutual contact turned out to be ... | 1998 | 9593202 |
| expression and secretion of defined cutinase variants by aspergillus awamori. | several cutinase variants derived by molecular modelling and site-directed mutagenesis of a cutinase gene from fusarium solani pisi are poorly secreted by saccharomyces cerevisiae. the majority of these variants are successfully produced by the filamentous fungus aspergillus awamori. however, the l51s and t179y mutations caused reductions in the levels of extracellular production of two cutinase variants by a. awamori. metabolic labelling studies were performed to analyze the bottleneck in enzym ... | 1998 | 9687432 |
| comparison of in vitro activities of the new triazole sch56592 and the echinocandins mk-0991 (l-743,872) and ly303366 against opportunistic filamentous and dimorphic fungi and yeasts. | the in vitro antifungal activities of sch56592, mk-0991, and ly303366 against 83 isolates of acremonium strictum, aspergillus flavus, aspergillus fumigatus, aspergillus terreus, bipolaris spp., blastomyces dermatitidis, cladophialophora bantiana, fusarium oxysporum, fusarium solani, histoplasma capsulatum, phialophora spp., pseudallescheria boydii, rhizopus arrhizus, scedosporium prolificans, and sporothrix schenckii were compared. the in vitro activities of these agents against 104 isolates of ... | 1998 | 9738049 |
| endogenous endophthalmitis by fusarium solani: an animal experimental model. | an experimental model of endophthalmitis by fusarium solani in immunocompetent mice that could be useful for evaluating the efficacy of different treatments and the pathogenicity of the fungus in ocular structures was established. five clinical isolates of f. solani were injected into the lateral tail vein of groups of 20 mice, in order to produce systemic infection with ocular infection. inocula of 5 x 10(6) conidia per mouse were used. the eyes of the animals that died were enucleated for hist ... | 1998 | 10075492 |
| antisporulating and respiration-inhibitory effects of essential oils on filamentous fungi. | sporulation of four species of filamentous fungi, namely aspergillus fumigatus, fusarium solani, penicillium expansum and rhizopus oryzae, was suppressed by gaseous contact with citron, lavender and thyme oils and, to a lesser extent, by that of perilla and tea tree oils. lemongrass and cinnamon bark oils were scarcely active. the antisporulating effect of the essential oils was not observed when they were applied as a solution, indicating that their vapours were the active form. moreover, expos ... | 1998 | 9916464 |
| in vitro activity of the new triazole voriconazole (uk-109,496) against opportunistic filamentous and dimorphic fungi and common and emerging yeast pathogens. | the in vitro antifungal activity of a new triazole derivative, voriconazole, was compared with those of itraconazole and amphotericin b against 67 isolates of aspergillus flavus, aspergillus fumigatus, bipolaris spp., fusarium oxysporum, fusarium solani, pseudallescheria boydii, rhizopus arrhizus, blastomyces dermatitidis, histoplasma capsulatum, and sporothrix schenckii. the in vitro activities of voriconazole were also compared with those of amphotericin b, fluconazole, and itraconazole agains ... | 1998 | 9431946 |
| a unique case of cryptococcus laurentii keratitis spread by a rigid gas permeable contact lens in a patient with onychomycosis. | we report the first case of fungal keratitis caused by cryptococcus laurentii, a nonneoformans species. | 1998 | 9436889 |
| delayed-onset fungal keratitis after endophthalmitis. | this study aimed to present two patients with delayed-onset, endogenous fungal keratitis after treatment for fungal endophthalmitis after cataract surgery. | 1998 | 9479284 |
| in-vitro activity of voriconazole, itraconazole and amphotericin b against filamentous fungi. | the in-vitro fungistatic and fungicidal activities of voriconazole were compared with those of itraconazole and amphotericin b. mics for 110 isolates belonging to 11 species of filamentous fungi were determined by a broth microdilution adaptation of the method recommended by the national committee for clinical laboratory standards. minimum lethal concentrations (mlcs) of the three antifungal agents were also determined. the mic ranges of the three compounds were comparable for aspergillus flavus ... | 1998 | 10052897 |
| carbon loss and germinability, viability, and virulence of chlamydospores of fusarium solani f. sp. phaseoli after exposure to soil at different ph levels, temperatures, and matric potentials. | abstract (14)c-labeled chlamydospores of fusarium solani f. sp. phaseoli were exposed to soil at 5, 15, 25, or 30 degrees c at ph 5 or 8 and water potential of -1 kpa or to soil at 0, -1, or -10 kpa at 25 degrees c at ph 6.9. total carbon loss was greatest at 25 or 30 degrees c at ph 8 and -1 kpa. (14)co(2) from respiration of chlamydospores and from soil microbes utilizing chlamydospore exudates accounted for the largest share of total carbon loss under all conditions. (14)(co)(2) from soil mic ... | 1998 | 18944984 |
| suppression of fusarium solani f. sp. phaseoli on bean by aluminum in acid soils. | abstract the severity of bean root rot caused by fusarium solani f. sp. phaseoli in vitro was studied with regard to exchangeable soil aluminum for 25 soil samples collected from northeastern honshyu island, japan. of these, 24 were andosols, typically acidic and of volcanic ash origin. disease severity was assessed based on the number of lesions produced by the pathogen on a 6-cm section of bean stem buried and incubated for 8 days at 25 degrees c in artificially infested soil samples. the numb ... | 1999 | 18944802 |
| modulation of neutrophil-mediated activity against the pseudohyphal form of candida albicans by granulocyte colony-stimulating factor (g-csf) administered in vivo. | renewed interest in neutrophil transfusions has emerged with the development and clinical use of granulocyte colony-stimulating factor (g-csf). g-csf not only increases neutrophil (polymorphonuclear leukocyte, pmnl) production but also modulates various physiological properties of pmnl. the effects of g-csf on pmnl-mediated fungicidal activity were evaluated by administration of g-csf (300 micrograms/day subcutaneously) to 5 healthy volunteers for 6 days. g-csf significantly enhanced pmnl-mediat ... | 1999 | 10191242 |
| interfacial binding of cutinase rather than its catalytic activity determines the steady state interfacial tension during oil drop lipid hydrolysis. | hydrolysis of triglycerides by cutinase from fusarium solani pisi causes in oil drop tensiometer experiments a decrease of the interfacial tension. a series of cutinase variants with amino acid substitutions at its molecular surface yielded different values of the steady state interfacial tension. this tension value poorly correlated with the specific activity as such nor with the total activity (defined as the specific activity multiplied by the amount of enzyme bound) of the cutinase variants. ... | 1999 | 10192932 |
| in vitro susceptibility of opportunistic fusarium spp. to essential oils. | the inhibitory effects of essential oils extracted from 10 indian plants were evaluated against five fungi. the plants used for extraction of essential oils were six species of the genus eucalyptus and ocimum basilicum, prosopis cineraria and derris indica. the fungi used in the experiments were fusarium solani, f. oxysporum, f. pallidoroseum, f. acuminatum and f. chlamydosporum. the susceptibility of the fusarium species was tested by the paper disc method and the serial dilution technique. the ... | 1999 | 10394856 |
| backbone dynamics of fusarium solani pisi cutinase probed by nuclear magnetic resonance: the lack of interfacial activation revisited. | the backbone dynamics of fusarium solani pisi cutinase has been studied by a variety of nuclear magnetic resonance experiments to probe internal motions on different time scales. the core of cutinase appears to be highly rigid. the binding site, including the oxyanion hole, is mobile on the microsecond to millisecond time scale, in contrast to the well-defined active site and preformed oxyanion hole elucidated by x-ray crystallography [martinez, c., de geus, p., lauwereys, m., matthyssens, g., a ... | 1999 | 10220318 |
| gallotannin hydrolysis by immobilized fungal mycelia in a packed bed bioreactor. | hydrolysis of gallotannin to gallic acid by immobilized mycelia of aspergillus niger mtcc 282, aspergillus fischerii mtcc 150, fusarium solani mtcc 350 and trichoderma viride mtcc 167 in a packed bed bioreactor was studied. fungal mycelia preinduced with 5 g l-1 gallotannin were immobilized in calcium alginate gel (1.5%) and the resultant beads were packed in a column to a bed volume of 175 mm3. gallotannin dissolved in distilled water was passed through the column and the eluate was recycled af ... | 1999 | 10355367 |
| nmr studies of fusarium solani pisi cutinase in complex with phosphonate inhibitors. | the backbone dynamics of fusarium solani pisi cutinase in complex with a phosphonate inhibitor has been studied by a variety of nuclear magnetic resonance experiments to probe internal motions on different time scales. the results have been compared with dynamical studies performed on free cutinase. in solution, the enzyme adopts its active conformation only upon binding the inhibitor. while the active site ser120 is rigidly attached to the stable alpha/beta core of the protein, the remainder of ... | 1999 | 10320324 |
| influence of he-ne laser irradiation of soybean seeds on seed mycoflora, growth, nodulation, and resistance to fusarium solani. | laser irradiation of soybean seeds for 3 min caused a clear reduction in the number of seed-borne fungi which became more pronounced as the irradiation time was extended. pretreatment of the seeds with methylene blue, methyl red and carmine enhanced the effect of laser. rhizoctonia solani, alternaria tenuissima, cercospora kikuchii and colletotrichum truncatum were completely eliminated when the seeds were pretreated with a dye and irradiated for 10 min. seed germination was stimulated on exposu ... | 1999 | 10983234 |
| effects of methylprednisolone and cyclosporine a on fungal growth in vitro. | the management of corneal transplants after mycotic keratitis often poses a therapeutic dilemma. clinicians are hesitant to use topical steroids because of their potential enhancement of fungal growth. this study seeks to evaluate the in vitro effects of methylprednisolone and cyclosporine a on the growth of various molds that often are responsible for keratomycoses. | 1999 | 10336034 |
| fungal keratitis as an indicator of hiv infection in africa. | a 1-year hospital-based study was undertaken on 212 patients at muhimbili medical centre (mmc) from october 1994 to october 1995. the objective was to determine the aetiology of corneal ulceration. a comparison of the prevalence of hiv infection between patients with fungal keratitis and those with non-fungal keratitis was included. bacterial infection (32.1%), and mycotic infection (15.1%) were the leading causes of corneal ulceration. there were 32 patients with fungal keratitis and 180 patien ... | 1999 | 10448232 |
| cloning and characterization of pl1 encoding an in planta-secreted pectate lyase of fusarium oxysporum. | a pectate lyase (pl1) from the tomato vascular wilt pathogen fusarium oxysporum f.sp. lycopersici was previously characterized, and evidence was obtained for its production in planta. the gene encoding pl1 was isolated from a genomic library of f. oxysporum f. sp. lycopersici. pl1 encodes a 240 amino-acid polypeptide with one putative n-glycosylation site and a 15 amino-acid n-terminal signal peptide. pl1 showed 89%, 67%, 55% and 56% identity with the products of the fusarium solani f.sp. pisi p ... | 1999 | 10022947 |
| comparison of e-test and broth microdilution methods for antifungal drug susceptibility testing of molds. | we compared the e test with a broth microdilution method, performed according to national committee for clinical laboratory standards document m27-a guidelines, for determining the in vitro susceptibilities of 90 isolates of pathogenic molds (10 absidia corymbifera, 10 aspergillus flavus, 10 aspergillus fumigatus, 10 aspergillus niger, 10 aspergillus terreus, 10 exophiala dermatitidis, 10 fusarium solani, 10 scedosporium apiospermum, 5 scedosporium prolificans, and 5 scopulariopsis brevicaulis). ... | 1999 | 10203509 |
| comparison of interferon-gamma, granulocyte colony-stimulating factor, and granulocyte-macrophage colony-stimulating factor for priming leukocyte-mediated hyphal damage of opportunistic fungal pathogens. | proinflammatory cytokines have been proposed as adjunctive therapeutic agents to enhance the host immune response during infections caused by opportunistic fungi. the study compared the differential in vitro priming effects of interferon-gamma (ifn-gamma), granulocyte colony-stimulating factor (g-csf), and granulocyte-macrophage colony-stimulating factor (gm-csf) on hyphal damage of opportunistic fungi mediated by isolated neutrophils (polymorphonuclear leukocytes, pmnl) and buffy coat cells (po ... | 1999 | 10068606 |
| comparative antimicrobial efficacy of multi-purpose lens care solutions using the fda's revised guidance document for industry: stand-alone primary criteria. | we evaluated six single-bottle, multi-purpose lens care solutions and a two component lens care system for disinfection efficacy according to the stand-alone primary criteria within the recently published u.s. food and drug administration (fda) guidelines. | 1999 | 10073638 |
| transformation of aspergillus awamori by agrobacterium tumefaciens-mediated homologous recombination. | agrobacterium tumefaciens is known to transfer part of its tumor-inducing (ti) plasmid to the filamentous fungus aspergillus awamori by illegitimate recombination with the fungal genome. here, we show that when this ti dna shares homology with the a. awamori genome, integration can also occur by homologous recombination. on the basis of this finding, we have developed an efficient method for constructing recombinant mold strains free from bacterial dna by a. tumefaciens-mediated transformation. ... | 1999 | 10385327 |
| treatment of murine fusariosis with sch 56592. | doses of 10 to 100 mg of the azole antifungal agent sch 5692/kg of body weight/day were studied in immunocompetent mice as therapy for systemic infection by fusarium solani. treatment was begun 1 h after intravenous infection and continued daily for 4 or 13 doses. prolongation of survival and organ clearance were dependent on both the dose and the duration of sch 56592 therapy, with the best results seen at 50 and 100 mg/kg/day. the results at the highest doses of sch 56592 used (50 or 100 mg/kg ... | 1999 | 10049271 |
| microdilution susceptibility testing of amphotericin b, itraconazole, and voriconazole against clinical isolates of aspergillus and fusarium species. | we compared the activities of amphotericin b, itraconazole, and voriconazole against clinical aspergillus (n = 82) and fusarium (n = 22) isolates by a microdilution method adopted from the national committee for clinical laboratory standards (nccls-m27a). rpmi 1640 (rpmi), rpmi 1640 supplemented to 2% glucose (rpmi-2), and antibiotic medium 3 supplemented to 2% glucose (am3) were used as test media. mics were determined after 24, 48, and 72 h. a narrow range of amphotericin b mics was observed f ... | 1999 | 10565912 |
| in vitro and in vivo experimental activities of antifungal agents against fusarium solani. | in the treatment of disseminated fusarium infections, amphotericin b either alone or in combination with flucytosine and rifampin is the drug therapy most frequently used. the efficacy of these antifungal drugs was evaluated in a murine disseminated-infection model, with five strains of fusarium solani. all the treatments were clearly ineffective. | 1999 | 10223945 |
| in vitro activity of syn-2869, a novel triazole agent, against emerging and less common mold pathogens. | the in vitro activity of syn-2869 was compared with that of amphotericin b and itraconazole. mics for 100 isolates of pathogenic molds belonging to 12 species were determined by a broth microdilution adaptation of the method recommended by the national committee for clinical laboratory standards. syn-2869 and itraconazole showed comparable, good activity against the dematiaceous molds cladophialophora bantiana, cladophialophora carrionii, exophiala dermatitidis, fonsecaea pedrosoi, phialophora p ... | 1999 | 10223947 |
| cutinase: from molecular level to bioprocess development. | this review analyzes the role of cutinases in nature and their potential biotechnological applications. the cloning and expression of a fungal cutinase, fusarium solani f. pisi, in escherichia coli and saccharomyces cerevisiae hosts are described. the three-dimensional structure of this cutinase is also analyzed and its function as a lipase is discussed and compared with other lipases. the biocatalytic applications of cutinase are described taking into account the preparation of different cutina ... | 1999 | 10556791 |
| subcutaneous fusarium foot abscess in a renal transplant patient. | fusarium species are ubiquitous plant and grain phytopathogens that rarely cause opportunistic infections in immunocompromised patients. while disseminated fusarium infections are almost always fatal, localized infections may be responsive to a combination of systemic antibiotic therapy and surgical debridement. we present a diabetic renal transplant patient who developed a foot abscess due to fusarium solani. infection persisted despite aggressive surgical debridement and a 3-month course of in ... | 1999 | 10349541 |
| interdigital intertrigo of the feet due to therapy-resistant fusarium solani. | we report a case of bilateral intertrigo of the third and fourth interdigital spaces of the feet in a 34-year-old immunocompetent senegalese male. a diagnosis of fusarium solani infection was made. systemic and topical therapy with terbinafine led to clinical but not mycological recovery. as this mould is potentially dangerous for immunodepressed subjects, early diagnosis and rigorous follow-up of skin diseases caused by this agent are advisable. | 1999 | 10559592 |
| localized oral fusarium infection in an aids patient with malignant lymphoma. | we report here a case of localized oral fusarium infection in an aids patient who developed an ulceration in the soft palate. fusarium solani was identified by histopathology and culture. we believe this to be the second reported case of oral fusarium infection in a patient with haematological malignancy and the first reported association of oral fusarium infection with aids. | 1999 | 10609534 |
| specific detection of fusarium species in blood and tissues by a pcr technique. | fusarium species are opportunistic nosocomial pathogens that often cause fatal invasive mycoses. we designed a primer pair that amplifies by pcr a fragment of a gene coding for the rrna of fusarium species. the dnas of the main fusarium species and neocosmospora vasinfecta but not the dnas from 11 medically important fungi were amplified by these primers. the lower limit of detection of the pcr system was 10 fg of fusarium solani dna by ethidium bromide staining. to test the ability of this pcr ... | 1999 | 10405380 |
| dna fingerprinting of fusarium solani isolates related to a cutaneous infection in a sea turtle. | random amplified polymorphic dna (rapd) fingerprinting was applied to fusarium solani from a cutaneous hyalohyphomycosis in a loggerhead sea turtle. a total of seven f. solani isolates were examined, three from culture collections and four from the turtle infection (one from the turtle's lesions and three from the sand of the tank in which the turtle was kept). the banding patterns of the isolates from culture collections were markedly different from the patterns of the isolates from both the tu ... | 1999 | 10421856 |
| tryptophan mediated photoreduction of disulfide bond causes unusual fluorescence behaviour of fusarium solani pisi cutinase. | the fluorescence signal of the single tryptophan residue (trp69) of fusarium solani pisi cutinase is highly quenched. however, prolonged irradiation of the enzyme in the tryptophan absorption band causes an increase of the tryptophan fluorescence quantum yield by an order of magnitude. by using a combination of nmr spectroscopy and chemical detection of free thiol groups with a sulfhydryl reagent we could unambiguously show that the unusual fluorescence behaviour of trp69 in cutinase is caused b ... | 1999 | 10462054 |
| antimicrobial comparison of a new multi-purpose disinfecting solution to a 3% hydrogen peroxide system. | we compared the antimicrobial activity of a new multi-purpose disinfection solution (opti-free express with aldox) to a 3% hydrogen peroxide disinfecting system. the antimicrobial ingredients in the new solution are polyquaternium-1 and myristamidopropyl dimethylamine. | 1999 | 10555736 |
| disinfecting action of a new multi-purpose disinfection solution for contact lenses. | the disinfection activity of a new multipurpose disinfection solution (opti-free express with aldox) was compared to several other contact lens disinfecting solutions. the new solution is preserved with polyquaternium-1 and myristamidopropyl dimethylamine. the other solutions included 3% hydrogen peroxide systems and multipurpose solutions (mps) preserved with polyhexamethylene biguanide. the products were tested for antimicrobial activity against staphylococcus spp., pseudomonas aeruginosa, ser ... | 1999 | 16303414 |
| [two cases of scalp wound colonization and respiratory tract by mycelial fungi]. | one case of colonization of a post-traumatic scalp wound by aspergillus nidulans and fusarium solani in a healthy adult patient is discussed. a second case of respiratory colonization by acrophialophora fusispora in a child with cystic fibrosis is presented. both cases are reported to illustrate the need of combined histologic and mycologic studies in evaluating patients in order to distinguish between colonization and invasion. | 2000 | 15762812 |
| treatment of postkeratitis fusarium endophthalmitis with amphotericin b lipid complex. | the authors report the first case of fusarium solani keratitis that progressed to fungal endophthalmitis and was successfully treated with amphotericin b lipid complex (ablc). | 2000 | 11095064 |
| differential expression of eight chitinase genes in medicago truncatula roots during mycorrhiza formation, nodulation, and pathogen infection. | expression of eight different chitinase genes, representing members of five chitinase classes, was studied in medicago truncatula roots during formation of arbuscular mycorrhiza with glomus intraradices, nodulation with rhizobium meliloti, and pathogen attack by phytophthora megasperma f. sp. medicaginis, fusarium solani f. sp. phaseoli (compatible interactions with root rot symptoms), ascochyta pisi (compatible, symptomless), and f. solani f. sp. pisi (incompatible, nonhost interaction). in the ... | 2000 | 10875337 |
| fungal keratitis in a soft contact lens wearer. | to describe a case of fungal keratitis in a soft contact lens wearer. | 2000 | 10946989 |
| successful treatment of fusarium solani ecthyma gangrenosum in a child with acute lymphoblastic leukemia in relapse. | 2000 | 10877182 | |
| rapid detection and identification of candida, aspergillus, and fusarium species in ocular samples using nested pcr. | a protocol for the rapid detection of fungal dna in ocular samples, derived from three species, candida albicans, aspergillus fumigatus, and fusarium solani, has been developed. two novel panfungal primers complementary to 18s rrna sequences present in all three species were designed. panfungal pcr was followed by three nested pcrs utilizing species-specific primers. pcr sensitivity ranged from 50 to 100 fg of free dna and between one and two c. albicans organisms. in addition, we also developed ... | 2000 | 10921948 |
| mixed infection caused by two species of fusarium in a human immunodeficiency virus-positive patient. | we report on a case of mixed infection caused by two species of fusarium in a human immunodeficiency virus-positive patient with lymphoma who was neutropenic due to chemotherapy. the patient showed the typical signs of a disseminated fusarial infection, with fusarium solani isolated from skin lesions and f. verticillioides isolated from blood. the report discusses how difficult it is to make an accurate diagnosis when an immunosuppressed patient is infected with more than one fungal species, esp ... | 2000 | 10970404 |
| effects of ionic surfactants used in reversed micelles on cutinase activity and stability. | the effects of aqueous surfactant solutions on the kinetics and stability of cutinase from fusarium solani pisi were studied. the surfactant sodium bis[2-ethylhexyl]ester sulfosuccinic acid (aot) acts as a pseudo-competitive inhibitor within a limited concentration range relative to the hydrolysis of short-chain p-nitrophenyl esters. for higher concentrations a hyperbolic mixed inhibition takes place. a pseudo-activation of hydrolysis in presence of aot and hexadecyltrimethyl-ammonium bromide (c ... | 2000 | 11004558 |
| invasive hyalohyphomycosis due to fusarium solani in a patient with acute lymphocytic leukemia. | 2000 | 11044201 | |
| an unusual pectate lyase from a bacillus sp. with high activity on pectin: cloning and characterization. | the gene pela encoding a pectate lyase from the strain bacillus sp. bp-23 was cloned and expressed in escherichia coli. the nucleotide sequence of a 1214 bp dna fragment containing pela gene was determined, revealing an orf of 666 nucleotides that encoded a protein of 23233 da. the deduced amino acid sequence of the encoded enzyme showed homology to pectate lyases a, b, c and d from fusarium solani, pel-3 and pelb from erwinia carotovora and pell from erwinia chrysanthemi. homology was also foun ... | 2000 | 10658655 |
| in vitro activities of a new lipopeptide antifungal agent, fk463, against a variety of clinically important fungi. | the in vitro antifungal activity and spectrum of fk463 were compared with those of amphotericin b, fluconazole, and itraconazole by using a broth microdilution method specified by national committee for clinical laboratory standards document m27-a (national committee for clinical laboratory standards, wayne, pa., 1997). fk463 exhibited broad-spectrum activity against clinically important pathogens including candida species (mic range, <==0.0039 to 2 microg/ml) and aspergillus species (mic range, ... | 2000 | 10602723 |
| microbial transformation of benzosampangine. | microbial transformation studies of the synthetic antifungal alkaloid benzosampangine (1) have revealed that 1 is metabolized by a number of microorganisms. using a standard two-stage fermentation technique absidia glauca (atcc 22752), cunninghamella blakesleeana (atcc 8688a), cunninghamella species (nrrl 5695), fusarium solani f. sp. cucurbitae (csih #c-5), and rhizopogon species (atcc 36060) each produced a beta-glucopyranose conjugate of benzosampangine (2). the identity of 2 was established ... | 2000 | 10757729 |
| deduced amino-acid sequence and possible catalytic residues of a novel pectate lyase from an alkaliphilic strain of bacillus. | the nucleotide sequence of the gene for a highly alkaline, low-molecular-mass pectate lyase (pel-15) from an alkaliphilic bacillus isolate was determined. it harbored an open reading frame of 672 bp encoding the mature enzyme of 197 amino acids with a predicted molecular mass of 20 924 da. the deduced amino-acid sequence of the mature enzyme showed very low homology (< 20.4% identity) to those of known pectinolytic enzymes in the large pectate lyase superfamily (the polysaccharide lyase family 1 ... | 2000 | 10759850 |
| genetic engineering of the fusarium solani pisi lipase cutinase for enhanced partitioning in peg-phosphate aqueous two-phase systems. | the fusarium solani pisi lipase cutinase has been genetically engineered to investigate the influence of c-terminal peptide extensions on the partitioning of the enzyme in peg-salt based aqueous two-phase bioseparation systems. seven different cutinase lipase variants were constructed containing various c-terminal peptide extensions including tryptophan rich peptide tags ((wp)(2) and (wp)(4)), positively ((rp)(4)) and negatively ((dp)(4)) charged tags as well as combined tags with tryptophan tog ... | 2000 | 10812184 |
| analysis of the dynamics of fungal communities in soil via fungal-specific pcr of soil dna followed by denaturing gradient gel electrophoresis. | a molecular method for profiling of fungal communities in soil was applied in experiments in soil microcosms, with two objectives, (1) to assess the persistence of two selected fungal species in soil, and (2) to analyze the response of the natural fungal community to a spill of sulphurous petrol in the same soil. to achieve the aims, two soil dna extraction methods, one originally designed for the direct extraction of bacterial community dna and the other one aimed to obtain fungal dna, were tes ... | 2000 | 11121612 |
| fusarium solani pisi cutinase. | cutinase from fusarium solani pisi has been studied extensively with respect to its structural and functional properties. the crystal structure of the enzyme was solved to high atomic resolution (1 angstrom), while data on structural dynamics have been obtained from detailed nmr studies. functional data were mainly derived from kinetic studies using substrate analogues that simplify the kinetic behaviour. the properties of wild-type cutinase are reviewed and discussed in relation with the effec ... | 2000 | 11099798 |
| assessment of growth of fusarium solani by cyclic voltammetry and possible bioanalytical applications. | fusarium solani, the fungus isolated from polyurethane waste scraps, was studied for its voltammetric response. the peak current values were obtained for different days and were plotted against time. this electrochemical method based on the voltammetric response showed all the four phases of the growth of the fungus. the growth curve obtained matched well with the conventional methodology, which is obtained by assessing the increase of dry weight of the organisms against time. the electrochemica ... | 2000 | 11219046 |
| three-dimensional structure of the catalytic core of acetylxylan esterase from trichoderma reesei: insights into the deacetylation mechanism. | acetylxylan esterase from trichoderma reesei removes acetyl side groups from xylan. the crystal structure of the catalytic core of the enzyme was solved at 1.9 a resolution. the core has an alpha/beta/alpha sandwich fold, similar to that of homologous acetylxylan esterase from penicillium purpurogenum and cutinase from fusarium solani. all three enzymes belong to family 5 of the carbohydrate esterases and the superfamily of the alpha/beta hydrolase fold. evidently, the enzymes have diverged from ... | 2000 | 11243887 |
| antigens of fusarium solani: types and criteria for immunochemical characterization. | there are no established methods for the preparation and standardization of fusarium solani antigens. this lack of standardization makes it difficult to use these antigens in allergenic diagnostic tests. | 2000 | 11270086 |
| effect of salts and surfactants on the partitioning of fusarium solani pisi cutinase in aqueous two-phase systems of thermoseparating ethylene oxide/propylene oxide random copolymer and hydroxypropyl starch. | an aqueous two-phase system composed by a thermoseparating random copolymer of ethylene oxide/propylene oxide 50/50 (%w/w), breox, and hydroxypropyl starch-reppal pes 100 was evaluated for the partitioning of fusarium solani pisi recombinant cutinase. the effect of several additives on the partitioning of pure cutinase was evaluated. micelles of sodium dodecanoate provided a ten-fold increase of the partitioning coefficient (k=9) and recovery yields of 60-75%. the phase diagrams of the systems c ... | 2000 | 11321518 |