Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| amino acid sequence at the allosteric site of sheep heart phosphofructokinase. | covalent modification of sheep heart phosphofructokinase with the affinity labeling reagent p-fluorosulfonyl[14c]benzoyl-5'-adenosine caused a loss of allosteric properties. this modification appears to occur at the binding site that is specific for the allosteric activators amp, camp, and adp (mansour, t.e., and colman, r.f. (1978) biochem. biophys. res. commun. 81, 1370-1376). in the current study, the site of modification has been demonstrated to be a lysine residue. a nonapeptide containing ... | 1980 | 6444412 |
| phosphofructokinase: complete amino-acid sequence of the enzyme from bacillus stearothermophilus. | the entire amino acid sequence of the protein subunit of phosphofructokinase from bacillus stearothermophilus has been established mainly by sequence analysis of cyanogen bromide fragments and of peptides derived from these fragments by further digestion with proteolytic enzymes. overlaps of the cyanogen bromide fragments as well as peptide sequences necessary to complement and to confirm tentative assignments within the larger peptide fragments were obtained from the sequences of selected pepti ... | 1980 | 6447595 |
| [microbiological control of autoclave procedures in odontologic practice]. | 1980 | 6986603 | |
| a simplified procedure for the isolation of bacterial polypeptide elongation factor ef-tu. | 1980 | 6992647 | |
| amino acid:trna ligases (ec 6.1.1..-). | 1980 | 7000546 | |
| stoichiometry and structure of a complex of acidic ribosomal proteins. | 1980 | 7003159 | |
| probing the limits of protein-amino acid side chain recognition with the aminoacyl-trna synthetases. discrimination against phenylalanine by tyrosyl-trna synthetases. | the specificity of the tyrosyl-trna synthetases from escherichia coli and bacillus stearothermophilus for tyrosine compared with phenylalanine has been determined by using samples of phenylalanine which have been scrupulously freed from tyrosine by either chemical or enzymic scavenging procedures. both kinetic measurements and product analyses give a value of 1 x 10(5)-2 x 10(5) for the preferential activation of tyrosine. combined with the known ratio of phenylalanine to tyrosine in rapidly gro ... | 1980 | 7006687 |
| use of the carbon dioxide laser in sterilization of endodontic reamers. | the object of this study was to test a new method of sterilizing endodontic instruments by using a carbon dioxide laser system. this was done by contaminating endodontic reamers with spores, exposing them to a co2 laser beam, and checking for their viability by standard microbiologic techniques. it was found that 100 percent of the spores were killed by the co2 laser. this holds promise as an effective method of sterilizing endodontic instruments in the future. | 1980 | 6766545 |
| d-glyceraldehyde-3-phosphate dehydrogenase. amino-acid sequence of the enzyme from the extreme thermophile thermus aquaticus. | 1. the amino acid sequence of d-glyceraldehyde-3-phosphate dehydrogenase from the extreme thermophile thermus aquaticus has been elucidated. 2. the polypeptide contains 332 amino acids and its sequence is 70% identical with that of the enzyme from the moderate thermophile bacillus stearothermophilus. 3. in contrast to less thermostable forms of the enzymes from b. stearothermophilus, pig, lobster and yeast, the t. aquaticus enzyme has only one cysteine residue, namely cysteine-149 which is requi ... | 1980 | 6773768 |
| a method for measuring the cleaning effect of flushing disinfectors. | a method is presented with which the mechanical cleaning effect of flushing disinfectors can be estimated independently of the thermal disinfecting effect of the hot flushing water. this makes it possible to specify the demands to be placed on the disinfecting effect of flushing with water of 85 degrees c or more. bacillus stearothermophilus spores suspended in faeces were used as indicators because of their non-sensitivity to the hot-water temperature. their elimination by flushing could thus b ... | 1980 | 6820026 |
| arginyl-transfer ribonucleic acid synthetase of bacillus stearothermophilus. purification and kinetic analysis. | arginyl-trna synthetase from bacillus stearothermophilus (nca 1518) has been purified 880-fold to apparent homogeneity as demonstrated by electrophoresis in the presence of sodium dodecyl sulphate. the molecular weight is 59 000 as confirmed by sephadex g-100 and by sucrose gradient ultracentrifugation. the enzyme is monomeric, no subunits were detected. its cognate trna induces an apparent increase in molecular weight suggesting the dimerisation of the enzyme. nevertheless, it is not obvious th ... | 1980 | 7358046 |
| enolase from bacillus stearothermophilus. physicochemical characterization and reconstitution of the active enzyme from dissociated subunits. | 1980 | 7372405 | |
| the mechanism of action of penicillin. penicillin acylates the active site of bacillus stearothermophilus d-alanine carboxypeptidase. | penicillin kills susceptible bacteria by specifically inhibiting the transpeptidase that catalyzes the final step in cell wall biosynthesis, the cross-linking of peptidoglycan. it was hypothesized (tipper, d., and strominger, j. (1965) proc. natl. acad. sci. u.s.a. 54, 1133-1141) that 1) penicillin is a structural analog of the acyl-d-alanyl-d-alanine terminus of the pentapeptide side chains of nascent peptidoglycan, and that 2) penicillin, by virtue of its highly reactive beta-lactam structure, ... | 1980 | 7372662 |
| phenotypic and genotypic characterization of some thermophilic species of bacillus. | some thermophilic species of bacillus were characterized using biochemical tests, antibiotic sensitivity, bacteriocin and bacteriophage sensitivity, esterase patterns, dna hybridization and % g+c content. the three caldo-active strains of bacilli isolated by heinen (1971) were compared with strains of b. stearothermophilus. eight of the strains examined showed characteristics which enabled them to be placed in the three main taxonomic groups suggested by walker & wolf (1971). two strains showed ... | 1980 | 7391819 |
| superoxide dismutase from bacillus stearothermophilus. complete amino acid sequence of a manganese enzyme. | superoxide dismutase from the thermophilic bacterium bacillus stearothermophilus is dimeric with a molecular weight of 45 487. it contains one atom manganese(iii) per dimeric molecule [brock, c. j., harris, j. i., & sato, s. (1976) j. mol. biol. 107, 175-178]. the subunits are identical, and the following primary structure comprising 203 amino acids has been determined: (formula: see text) the enzyme is identical in 60% of its residues with the escherichia coli b manganese enzyme [steinman, h. m ... | 1980 | 7397109 |
| purification and some properties of 6-phosphoglucose isomerase from bacillus caldotenax. | 6-phosphoglucose isomerase [ec 5.3.1.9] was purified from bacillus caldotenax. the isomerase shared many common properties with the isomerase from b. stearothermophilus, i.e, ph and temperature optima, thermostability, competitive inhibition by 6-phosphogluconate and pi, and amino acid composition. the enzyme activity of the former, however, was lower than that of the latter. the molecular weight of the b. caldotenax isomerase was estimated to be 202,000-204,000 by gel filtration and electrophor ... | 1980 | 7400125 |
| purification and properties of polygalacturonic acid trans-eliminase from bacillus stearothermophilus. | a strain of thermophilic bacteria, bacillus stearothermophilus, with pectolytic activity has been isolated. it produced an endo-polygalacturonic acid trans-eliminase (endo-pate, ec 4.2.2.2) extracellularly when grown at 65 degrees c on a pectic acid medium. the pate was purified 62-fold by the rapid affinity chromatographic method on a sepharose-polygalacturonamide linked matrix. the absorbed pate was eluted from the column with a continuous gradient of 0-10(-3)m ethylenediaminetetraacetic acid ... | 1980 | 7407714 |
| d-glyceraldehyde-3-phosphate dehydrogenase. the purification and characterisation of the enzyme from the thermophiles bacillus stearothermophilus and thermus aquaticus. | 1. d-glyceraldehyde-3-phosphate dehydrogenase from two thermophilic bacteria has been purified by procedures including affinity chromatography on nad+-sepharose. 2. methods for making nad+-free enzyme are also described. 3. both the holo and apo forms of the enzyme from bacillus stearothermophilus have been crystallised. 4. the enzymes are tetrameric and composed of four chemically identical polypeptide chains of molecular weight 36,000. 5. the enzymes are much more stable to heat than their cou ... | 1980 | 7408867 |
| d-glyceraldehyde-3-phosphate dehydrogenase. complete amino-acid sequence of the enzyme from bacillus stearothermophilus. | 1. the complete amino acid sequence of d-glyceraldehyde-3-phosphate dehydrogenase from the moderate thermophile bacillus stearothermophilus has been determined. 2. this has been achieved largely by the automated sequence analysis of large fragements derived by chemical cleavage with cyanogen bromide, bnps-skatole [the product of reaction between n-bromosuccinimide and 2-(nitrophenyl-sulphenyl)-3-methylindole] and hydroxylamine and enzymic hydrolysis with trypsin at arginine residues. 3. the sequ ... | 1980 | 7408868 |
| heat stability of a tetrameric enzyme, d-glyceraldehyde-3-phosphate dehydrogenase. | the tetrameric enzyme d-glyceraldehyde-3-phosphate dehydrogenase from the moderate thermophile bacillus stearothermophilus is more stable to thermal denaturation than its counterpart from lobster muscle [harris et al. (1980) eur. j. biochem. 108, 535-547]. extra buried ionic bonds between subunits of the thermophilic enzyme make an important contribution to thermal stabilisation. further stabilisatio of the tetrameric enzyme is derived from additional hydrophobic interactions between the s-loops ... | 1980 | 7408869 |
| mechanism of protein synthesis inhibition during stationary phase in bacillus stearothermophilus. | the appearance of a protein (association factor i) in ribosomes from bacillus stearothermophilus at stationary phase of growth is described. association factor i is present on 30s subunits and 30s-50s ribosomal couples, but not on 50s subunits. this protein is responsible for the low levels of polyphenylalanine synthesis shown by stationary phase ribosomes. association factor i is able to bind to free 30s-50s ribonsomal couples but not to polysomes, and exerts its effect by inhibiting the initia ... | 1980 | 7412766 |
| effect of restrictive temperature on cell wall synthesis in a temperature-sensitive mutant of bacillus stearothermophilus. | a temperature-sensitive mutant of bacillus stearothermophilus, ts-13, was unable to grow above 58 degrees c, compared to 72 degrees c for the wild type. actively growing ts-13 cells lysed within 2 h when exposed to a restrictive temperature of 65 degrees c. peptidoglycan synthesis stopped within 10 to 15 min postshift before a shut down of other macromolecular syntheses. composition of preexisting peptidoglycan was not altered, nor was new peptidoglycan of aberrant composition formed. no signifi ... | 1980 | 7419492 |
| [possibility of dna-ligase participation in regulating the synthesis and degradation of heat-damaged dna in permeable bacillus stearothermophilus cells]. | the chromosome of bacillus stearothermophilus was found to contain apurine regions which were repaired mainly by excisions. in the permeable cells of the thermophilic bacterium, the observed intensive degradation and resynthesis of dna were regulated by active dna ligase. in this case, nad (the cofactor of ligase) sharply inhibited the atp-dependent and independent synthesis of dna. apparently, dna ligase may be involved in the regulation of degradation and resynthesis of dna with thermal lesion ... | 1980 | 7442567 |
| formation and energy transfer of a fluorescent derivative of b. stearothermophilus glyceraldehyde-3-phosphate dehydrogenase. | the active site carboxymethylated glyceraldehyde-3-phosphate dehydrogenase from b. stearothermophilus when irradiated with ultraviolet light in the presence of nad gives rise to a fluorescent derivative closely similar to that obtained from the muscle enzyme in fluorescence properties. a radiationless energy transfer also occurs between the tryptophan residues of the enzyme protein and the new fluorophore, as for the muscle enzyme. quantitative determinations of the quantum yields and calculatio ... | 1980 | 7448190 |
| [functional model of mn-superoxide dismutase from bacillus stearothermophilus]. | 1980 | 7448234 | |
| purificaton of the pyruvate dehydrogenase multienzyme complex of bacillus stearothermophilus and resolution of its four component polypeptides. | 1. the pyruvate dehydrogenase complex was purified from bacillus stearothermophilus in high yield. the specific activity (about 40nkat/mg of protein) was substantially lower than that of the pyruvate dehydrogenase complex from escherchia coli (about 570nkat/mg of protein) measured at 30 degrees c under the same conditions. 2. the relative molecular masses of the four types of polypeptide chain i the complex were estimated by means of sodium dodecyl sulphate/polyacrylamide-gel electrophoresis to ... | 1980 | 7458900 |
| [principles of steam sterilization. comparison of biological and chemical indicators]. | 1980 | 7459030 | |
| purification of an nadh-(dichlorophenol-indophenol) oxidoreductase from bacillus stearothermophilus. | an nadh-(dichlorophenol-indophenol) oxidoreductase was purified 104-fold and in 25% overall yield from the thermophilic bacterium bacillus stearothermophilus, strain ph24. after solubilization in 2m-nacl at 70 degrees c, the enzyme was purified by ion-exchange and hydroxyapatite chromatography, followed by affinity chromatography on immobilized cibacron blue 3ga. the purified enzyme had a mol.wt. of 43 000 and had an absorption spectrum characteristic of flavoprotein. the enzyme activity was enh ... | 1980 | 7236205 |
| thermostability and aliphatic index of globular proteins. | a statistical analysis shows that the aliphatic index, which is defined as the relative volume of a protein occupied by aliphatic side chains (alanine, valine, isoleucine, and leucine), of proteins of thermophilic bacteria is significantly higher than that of ordinary proteins. the index may be regarded as a positive factor for the increase of thermostability of globular proteins. | 1980 | 7462208 |
| the use of various immobilized-triazine affinity dyes for the purification of 6-phosphogluconate dehydrogenase from bacillus stearothermophilus. | 1. 6-phosphogluconate dehydrogenase from bacillus stearothermophilus was purified approximately 260-fold on triazine-immobilized dye columns to a final specific activity of 54 mumol of nadp+ reduced/min per mg of protein and an overall yield of 62%. 2. an investigation of the capacities of different triazine dyes that inhibit 6-phosphogluconate dehydrogenase was carried out. cibacron blue f3g-a and procion red he-3b strongly inhibited the enzyme in free solution and were therefore chosen as the ... | 1980 | 7470099 |
| amino acid activation in crystalline tyrosyl-trna synthetase from bacillus stearothermophilus. | 1981 | 7265210 | |
| functional organization of the large ribosomal subunit of bacillus stearothermophilus. | bacillus stearothermophilus 50 s ribosomal subunits active in polyphenylalanine (polyphe) synthesis were reconstituted from a mixture of purified proteins and rna. proteins were omitted one at a time, and the resulting particles were examined by sucrose gradient sedimentation and assayed for polyphe synthesis, peptidyltransferase activity, and in some cases binding of elongation factor ef-g and gtp, and association with a (20 s . phe-trna . poly(u)) complex. based on their effect on polyphe synt ... | 1981 | 7275969 |
| fidelity of dna replication in vivo. | 1981 | 7284002 | |
| [isolation and properties of two dna-polymerases from bacillus stearothermophilus cells]. | two dna-polymerases (1 and 2) were isolated from the cells of bacillus stearothermophilus and their properties were investigated. the isolation procedure included separation of nucleic acids on deae-cellulose, affinity chromatography on dna-agarose and chromatography on deae-sephadex. dna-polymerase 1 was purified 150-fold; its molecular weight and ph optimum are 135 000 and 7.3, respectively. dna-polymerase 2 was purified 1000-fold; its molecular weight and ph optimum are 95 000 and 9.0, respec ... | 1981 | 7284483 |
| uracil-dna glycosylase from bacillus stearothermophilus. | 1981 | 7297702 | |
| the effect of electron transport on the kinetics of the manganese-containing superoxide dismutase from bacillus stearothermophilus. | 1981 | 7311574 | |
| sequence and secondary structure of the colicin fragment of bacillus stearothermophilus 16s ribosomal rna. | the sequence and the position of post-transcriptionally modified residues of the 3' -terminal end of bacillus stearothermophilus 16s ribosomal rna have been determined from the fragment that is cleaved off by bacteriocin treatment. the fragment contains 52 nucleotides, as compared to the 49 nucleotides of the corresponding fragment from e. coli ribosomes, the additional nucleotides are present in the sequence ucu very next to the 3' -terminus as was published earlier (1). the remainder of the se ... | 1981 | 7312621 |
| [action of gamma and uv irradiation on dna synthesis in permeable bacillus stearothermophilus cells]. | 1981 | 7313086 | |
| bacillus stearothermophilus from saudi arabian soils. | ten thermophilic gram-positive bacteria were isolated from various soils of saudi arabia. the strains are spore-forming rods belonging to the species bacillus stearothermophilus. the cells are motile, strictly aerobic, catalase and oxidase positive. the sporangia appear to be swollen and their position varies from terminal in some to sub-terminal in others. the thermal stability of some enzymes of these bacteria was investigated; extracellular alpha-amylase appears to be very sensitive to ph and ... | 1981 | 7319400 |
| purification and crystallization of protein complex from bacillus stearothermophilus ribosomes. | 1981 | 7338915 | |
| the effect of nucleotide presaturation on the chromatographic behaviour of 6-phosphogluconate dehydrogenase from bacillus stearothermophilus on immobilized triazine dyes. | 1981 | 7340817 | |
| amino acid sequence of a dodecapeptide from the substrate-binding region of the l-lactate dehydrogenase from lactobacillus curvatus, lactobacillus xylosus and bacillus stearothermophilus. | the amino acid sequence of dodecapeptides from the substrate-binding region of 3 bacterial l-lactate dehydrogenases (lactobacillus xylosus, lactobacillus curvatus and bacillus stearothermophilus) were determined. they show a very high homology to the sequences of the corresponding known animal enzymes. there is, however, an essential difference between the sequences of pro- and eucaryotic enzymes: the asn residue in position 166, common to all eucaryotes, is replaced by serine in lactobacilli an ... | 1981 | 7346373 |
| purification and properties of deoxyribonucleic acid polymerase from bacillus stearothermophilus. | deoxyribonucleic acid polymerase i was purified from bacillus stearothermophilus to 50 to 70% homogeneity. its molecular weight was 76,000. the enzyme was insensitive to sulfhydryl blocking agents and showed maximal activity at 60 degrees c, ph 8 to 9, 0.25 m kcl, and 0.02 m mgso4. the rate of heat inactivation of the deoxyribonucleic acid polymerase followed first-order kinetics with a half-life of 90 min at 60 degrees c; the addition of 0.05% bovine serum albumin protected the enzyme, which co ... | 1981 | 7462144 |
| sequence homology in the amino-terminal and active-site regions of thermolabile glyceraldehyde-3-phosphate dehydrogenase from a thermophile. | the unusual thermolability of glyceraldehyde-3-phosphate dehydrogenase from the facultative thermophile bacillus coagulans ku (crabb et al., biochemistry 16:4840-4847, 1977) has provided the first opportunity to study a homologous enzyme from the same genus that exhibits a marked difference in thermostability. in pursuit of the structural bases for the thermostability of proteins, the sequences of the amino terminus (residues 1 through 27) and the active-site cysteine cyanogen bromide peptide (r ... | 1981 | 7462149 |
| mass spectrometric sequence studies of a superoxide dismutase from bacillus stearothermophilus. | a partial sequence of the manganese-containing superoxide dismutase from bacillus stearothermophilus has been determined by mass spectrometry. a new fragmentation reaction at n-terminal proline is described and an 'in-chain' fragmentation, also occurring at proline, has been observed. there is some evidence that the latter cleavage may also occur at other residues. some differences from the classically determined sequence are reported, and the validity of these observations is discussed. | 1981 | 7202415 |
| [growth of obligate-thermophilic bacteria on a medium with paraffin]. | the obligate thermophilic bacteria bacillus stearothermophilus (the optimal growth temperature 55-73 degrees c) and thermus ruber (the optimal growth temperature 60 degrees c) were isolated from hot water springs of the kunashir island for the first time in a liquid mineral medium with paraffin. some of the bacteria belonging to bac. stearothermophilus grew at a high rate in a liquid medium with paraffin (strain 16); other strains (12a, 12b) were capable of growing only together with thermus rub ... | 1981 | 7219220 |
| deoxyribonucleic acid modification methylase from bacillus stearothermophilus. | a modification methylase was isolated from bacillus stearothermophilus 1503-4r (bst 1503i) and purified to homogeneity. the enzyme is an acidic protein and composed of a subunit with a molecular weight of 105 000, and only the tetrameric form was detected in solution. the methylase exhibited maximal activity between 54 and 61 degrees c and between ph 8.1 and 9.3. in contrast to bst 1503i endonuclease [catterall, j.f., & welker, n. e. (1977) j. bacteriol. 129, 1110-1120], the methylase is complet ... | 1981 | 7225321 |
| bacillus stearothermophilus spore suspensions: effect of storage conditions and time on viability and moist heat resistance. | 1981 | 7229838 | |
| limited proteolysis of the penicillin-sensitive d-alanine carboxypeptidase purified from bacillus subtilis membranes. active water-soluble fragments generated by cleavage of a cooh-terminal membrane anchor. | 1981 | 6780558 | |
| primary structure of the cooh-terminal membranous segment of a penicillin-sensitive enzyme purified from two bacilli. | d-alanine carboxypeptidase is a penicillin-sensitive intrinsic membrane enzyme which is composed of a hydrophilic nh2-terminal catalytic domain (mr congruent to 45,000 to 47,000) and a cooh-terminal membranous segment (approximately 20 to 30 amino acids in length) (waxman, d. j., and strominger, j. l. (1979) j. biol. chem. 254, 4863-4875; waxman, d. j., and strominger, j. l. (1981) j. biol. chem. 256, 2059-2066). the primary structures of the cooh-terminal 30 amino acids of two d-alanine carboxy ... | 1981 | 6780559 |
| properties of the virulent form of a mitomycin c- or temperature-induced thermophilic bacteriophage. | a virulent bacteriophage was isolated from lysates of bacillus stearothermophilus strain nu-10 induced either by treatment with mitomycin c or by shifts in temperature. optimum conditions for induction, morphology and other properties of the virus are described. chloroform treatment and shifts in temperature of producer cells released approximately similar amounts of phage as did mitomycin induction, suggesting an effect on release rather than on synthesis of the virus. | 1981 | 6790667 |
| monitoring steam sterilization of surgical instruments: a dilemma. | the study of two biological indicators in monitoring "flash" sterilization demonstrated that indicator construction often leads to a false interpretation of spore survival. | 1981 | 6792986 |
| binding of zinc to escherichia coli phenylalanyl transfer ribonucleic acid synthetase. comparison with other aminoacyl transfer ribonucleic acid synthetases. | 1981 | 6794600 | |
| immunochemical evidence of homologies among 50 s ribosomal proteins of bacillus stearothermophilus and escherichia coli. | antibodies prepared against individual 50 s ribosomal subunit proteins from escherichia coli were reacted with 70 s ribosomal proteins from bacillus stearothermophilus in order to identify homologous protein pairs. b. stearothermophilus proteins were separated by two-dimensional polyacrylamide gel electrophoresis and transferred electrophoretically to diazobenzyloxymethyl paper to which they became covalently attached. the paper was then washed with antiserum followed by radioactive protein a, a ... | 1981 | 7024264 |
| alternative pathways for editing non-cognate amino acids by aminoacyl-trna synthetases. | evidence is presented that the editing mechanisms of aminoacyl-trna synthetase operate by two alternative pathways: pre-transfer, by hydrolysis of the non-cognate aminoacyl adenylate; post-transfer, by hydrolysis of the mischarged trna. the methionyl-trna synthetases from escherichia coli and bacillus stearothermophilus and isoleucyl-trna synthetase from e. coli, for example, are shown to reject misactivated homocysteine rapidly by the pre-transfer route. a novel feature of this reaction is that ... | 1981 | 7024910 |
| the crystallization of ribosomal proteins from the 50 s subunit of the escherichia coli and bacillus stearothermophilus ribosome. | several individual intact ribosomal proteins purified from bacterial sources under mild conditions have been crystallized. a number of these are suitable candidates for three-dimensional structural studies by x-ray diffraction techniques. data collection to 3 a resolution for one of these proteins is in progress. | 1981 | 7028741 |
| the l7/l12 stalk, a conserved feature of the prokaryotic ribosome, is attached to the large subunit through its n terminus. | 1981 | 7028993 | |
| the complete amino acid sequence of human muscle glyceraldehyde 3-phosphate dehydrogenase. | 1981 | 7030790 | |
| secondary structure model for 23s ribosomal rna. | a secondary structure model for 23s ribosomal rna has been constructed on the basis of comparative sequence data, including the complete sequences from e. coli. bacillus stearothermophilis, human and mouse mitochondria and several partial sequences. the model has been tested extensively with single strand-specific chemical and enzymatic probes. long range base-paired interactions organize the molecule into six major structural domains containing over 100 individual helices in all. regions contai ... | 1981 | 7031608 |
| intramolecular coupling of active sites in the pyruvate dehydrogenase multienzyme complexes from bacterial and mammalian sources. | a simple method was developed for assessing the intramolecular coupling of active sites in the lipoate acetyltransferase (e2) component of the pyruvate dehydrogenase multienzyme complexes from escherichia coli, bacillus stearothermophilus and ox heart and pig heart mitochondria. samples of enzyme complex were prepared in which the pyruvate decarboxylase (e1) component was selectively and partly inhibited by treatment with increasing amounts of a transition-state analogue, thiamin thio-thiazolone ... | 1981 | 7032507 |
| phosphofructokinases from lactobacteriaceae. ii. purification and properties of phosphofructokinase from streptococcus thermophilus. | phosphofructokinase (atp : d-fructose-6-phosphate 1-phosphotransferase, ec 2.7.1.11) from streptococcus thermophilus has been purified. it is a tetramer composed of identical subunits of molecular weight 36 000 and exhibits michaelis-menten kinetics. compared to the phosphofructokinases from taxonomically related bacteria, the enzyme from s. thermophilus is more stable at high temperatures. in addition, it has been demonstrated that the phosphofructokinases from lactobacteria and also from baci ... | 1981 | 6457643 |
| the stereochemical course of phosphoryl transfer catalysed by bacillus stearothermophilus and rabbit skeletal-muscle phosphofructokinase with a chiral [16o,17o,18o]phosphate ester. | bacillus stearothermophilus and rabbit skeletal-muscle phosphofructokinases catalyse the transfer of the chiral [16o,17o,18o]phosphoryl group from d-fructose 1[(s)-16o,17o,18o],6-bisphosphate to adp with inversion of configuration at the phosphorus atom. d-fructose 1[(s)-16o,17o,18o],-bisphosphate was synthesized in situ from sn-glycerol 3[(s)-16o,17o,18o]phosphate. the simplest interpretation of these results is that the phosphoryl group is transferred between substrates in the enzyme substrate ... | 1981 | 6462132 |
| isolation and characterization of antibiotic resistance plasmids from thermophilic bacilli and construction of deletion plasmids. | ten plasmids were isolated as covalently closed circular deoxyribonucleic acid from antibiotic-resistant thermophilic bacteria. of the 10 plasmids tested, 2 could transform bacillus subtilis, yielding resistance to specific antibiotics. plasmid ptb20 (2.8 x 10(6) daltons, approximately 24 copies per chromosome) specifies resistance to tetracycline (tcr), whereas ptb19 (17.2 x 10(6) daltons, approximately 1 copy per chromosome) renders the host resistant to both kanamycin and tetracycline (kmrtcr ... | 1981 | 6263856 |
| the binding of nucleotides and metal ions to elongation factor tu from bacillus stearothermophilus as studied by equilibrium dialysis. | ef-tu from b. stearothermophilus binds divalent metal ions even in the absence of guanine nucleotides. the association constants necessary for characterizing the multiple equilibria between ef-tu, gdp and the divalent ions magnesium and manganese were determined by equilibrium dialysis. the constants are 4.6 x 10(4) m-1 and 5.4 x 10(5) m-1 for the binding of mg2 and 1.0 x 10(5) m-1 and 1.1 x 10(6) m-1 for the binding of mn2 to ef-tu and ef-tu . gdp, respectively. in the absence of divalent ions ... | 1981 | 6268521 |
| secondary structure of prokaryotic 5s ribosomal ribonucleic acids: a study with ribonucleases. | the structures of 5s ribosomal rnas from escherichia coli and bacillus stearothermophilus were examined by using ribonucleases a, t1, and t2 and a double helix specific cobra venom ribonuclease. by using both 5' and 3'-32p-end labeling methods and selecting for digested but intact 5s rna molecules, we were able to distinguish between primary and secondary cutting positions and also to establish the relative degree of cutting. the data reveal the predicted similarities of the higher order structu ... | 1981 | 6274397 |
| [microbial resistance to formaldehyde. ii. dependence of the microbicidal effect upon the concentration of and the period of exposure to formaldehyde (author's transl)]. | 1981 | 6275632 | |
| stringent response of bacillus stearothermophilus: evidence for the existence of two distinct guanosine 3',5'-polyphosphate synthetases. | bacillus stearothermophilus reacted to pseudomonic acid-induced inhibition of isoleucine-transfer ribonucleic acid (rna) acylation and to energy downshift caused by alpha-methylglucoside addition with accumulation of guanosine 3',5'-polyphosphates [(p)ppgpp] and restriction of rna synthesis. in vitro studies indicated that (p)ppgpp was synthesized by two different enzymes. one enzyme, (p)ppgpp synthetase i, was present in the ribosomal fraction, required the addition of a ribosome-messenger rna- ... | 1981 | 6161916 |
| the stringent response to unacylated trna, energy-and temperature-downshift in bacillus stearothermophilus. | the response of the thermophile bacillus stearothermophilus to inhibition of trna acylation, energy starvation and temperature downshift was characterized. we found that b. stearothermophilus, like other prokaryotic organisms, reacts with the so-called stringent response, which includes the accumulation of the unusual nucleotides guanosine 3',5' bis (diphosphate) [ppgpp] and guanosine 3'-diphosphate, 5'-tri-phosphate [pppgpp] and concomitantly the reduction of rna synthesis and growth rate. the ... | 1981 | 6164348 |
| reconstitution of biologically active 50s ribosomal subunits with artificial 5s rna molecules carrying disturbances in the base pairing within the molecular stalk. | bacillus stearothermophilus 50s ribosomal subunits were reconstituted in vitro using artificial 5s rna molecules constructed by combining parts of major and minor type (raué et al. (1976) europ. j. biochem. 68, 169-176) b. licheniformis 5s rna. the artificial 5s rna molecules carry defined disturbances (a.c juxtapositions and extra g.u pairs) in the base pairing between the 5'- and 3'-terminal sequences of the molecule (the molecular stalk region). the biological activity of the reconstituted su ... | 1981 | 6164987 |
| fluorescence modification of escherichia coli 5s rna. | reaction of 5s rna with chlorocetaldehyde leads to the conversion of unpaired adenines to the fluorescent 1,n6-etheno-adenine derivatives. up to 16 of the 23 adenines in free 5s rna can be modified, the fastest reacting are a29, a34, a57-59. partial modification of adenines in this area results in a 20% reduction in the efficiency of 5s rna incorporation into 50s subunits during reconstitution and a 15% reduction in the activity of these subunits in peptide synthesis. fluorescence from 1,n6-ethe ... | 1981 | 6169004 |
| tests on a low temperature steam and formaldehyde autoclave: the miniclave 80. | 1981 | 6174581 | |
| evaluation of a prototype micro-electronic autoclave cycle integrator. | instability and non-uniformity of spore preparations and the non-conformity of chemical indicators to the temperature coefficients of spore inactivation are problems associated with current methods of autoclave cycle monitoring. a prototype micro-electronic instrument, which largely overcomes these problems, is described. it monitors autoclave cycles in terms of the integral f0 and nabla functions. its thermometric and integrating accuracy is demonstrated. a discussion of the problems associated ... | 1981 | 6111601 |
| phosphofructokinase: structure and control. | phosphofructokinase from bacillus stearothermophilus shows cooperative kinetics with respect to the substrate fructose-6-phosphate (f6p), allosteric activation by adp, and inhibition by phosphoenolpyruvate. the crystal structure of the active conformation of the enzyme has been solved to 2.4 a resolution, and three ligand-binding sites have been located. two of these form the active site and bind the substrates f6p and atp. the third site binds both allosteric activator and inhibitor. the comple ... | 1981 | 6115424 |
| evaluation of glycine as an inactivator of glutaraldehyde. | glycine was evaluated as an inactivator of the sporicidal activity of glutaraldehyde. spores from glucose depleted cultures of bacillus stearothermophilus grown in a chemically defined medium were used. when glycine is used as an inactivator of glutaraldehyde, it lowers the ph value of the solution. glycine 1% failed to inactivate 0.5% or higher concentrations of alkaline glutaraldehyde in sporicidal studies. if viable counts cannot be performed within the first hour after inactivation, the con ... | 1982 | 6124593 |
| a comparison of heat resistance in commercially available bacillus stearothermophilus spore preparations used for monitoring steam sterilization. | 1982 | 6124601 | |
| binding site of ribosomal proteins on prokaryotic 5s ribonucleic acids: a study with ribonucleases. | the binding sites of ribosomal proteins l18 and l25 on 5s rna from escherichia coli were probed with ribonucleases a, t1, and t2 and a double helix specific cobra venom endonuclease. the results for the protein-rna complexes, which were compared with those for the free rna [douthwaite, s., & garrett, r. a. (1981) biochemistry 20, 7301--7307], reveal an extensive interaction site for protein l18 and a more localized one for l25. generally comparable results, with a few important differences, were ... | 1982 | 6178424 |
| transformation of bacillus stearothermophilus with plasmid dna and characterization of shuttle vector plasmids between bacillus stearothermophilus and bacillus subtilis. | a thermophilic bacterium bacillus stearothermophilus ifo 12550 (atcc 12980) was transformed with each of the following plasmids, pub110 (kanamycin resistance, kmr), ptb19 (kmr and tetracycline resistance [tcr]), and its derivative ptb90 (kmr tcr), by the protoplast procedure in the presence of polyethylene glycol at 48 degrees c. the transformation frequencies per regenerant for pub110, ptb19, and ptb90 were 5.9 x 10(-3), 5.5 x 10(-3), and 2.0 x 10(-1), respectively. among these plasmids, ptb90 ... | 1982 | 6277855 |
| limited proteolysis and proton nmr spectroscopy of bacillus stearothermophilus pyruvate dehydrogenase multienzyme complex. | the pyruvate dehydrogenase multienzyme complex of bacillus stearothermophilus was treated with chymotrypsin at ph 7 and 0 degrees c. loss of the overall catalytic activity lagged behind the rapid cleavage of the lipoate acetyltransferase polypeptide chains, whose apparent mr fell from 57 000 to 45 000 as judged by sodium dodecylsulphate/polyacrylamide gel electrophoresis. the inactive chymotrypsin-treated enzyme had lost the lipoic-acid-containing regions of the lipoate acetyltransferase chains, ... | 1982 | 6282590 |
| wet and dry bacterial spore densities determined by buoyant sedimentation. | the wet densities of various types of dormant bacterial spores and reference particles were determined by centrifugal buoyant sedimentation in density gradient solutions of three commercial media of high chemical density. with metrizamide or renografin, the wet density values for the spores and permeable sephadex beads were higher than those obtained by a reference direct mass method, and some spore populations were separated into several density bands. with percoll, all of the wet density value ... | 1982 | 6285824 |
| 6-phosphogluconate dehydrogenase from bacillus stearothermophilus. | 1982 | 6292662 | |
| acetate kinase from bacillus stearothermophilus. | 1982 | 6296602 | |
| cold lability and dissociation of the f1-atpase from bacillus stearothermophilus. | 1982 | 6462141 | |
| [isolation, purification and characteristics of alpha-amylase from bacillus stearothermophilus]. | 1982 | 6189370 | |
| molecular weight and amino acid composition of a thermostable lytic endopeptidase. | a thermostable lytic endopeptidase from bacillus stearothermophilus 1503-4r was purified 14,500-fold, with a 34% recovery of lytic activity. the enzyme is a basic protein (pi, 9.7) with a molecular weight of 15,100 and is composed of approximately 129 amino acid residues. | 1982 | 7037751 |
| the nucleotide sequence of the structural gene for escherichia coli tryptophanyl-trna synthetase. | the complete nucleotide sequence of trps, the structural gene for escherichia coli tryptophanyl-trna synthetase, was determined using a plasmid carrying the structural gene. from the single open reading frame of correct length and orientation we deduced an amino acid sequence consistent with the amino acid composition of the purified protein. in addition, previously sequenced peptides representing 52% of the protein were readily aligned with regions of the deduced sequence. the deduced amino aci ... | 1982 | 7042706 |
| partition of intermediates of triosephosphate isomerase: slow conformational changes precede enolization and follow product release. | 1982 | 7044418 | |
| [thermal resistance of the spores of a bac. stearothermophilus culture used for the preparation of bioindicators]. | thermostability of the spores of bac. stearothermophilus in ampoules and capillaries in concentrations of 10(9), 10(8) and 10(6) cells per 1 ml of sodium chloride isotonic solution was determined at 119 to 124 degrees c with an interval of 1 degree c and an exposure time of 5, 10, 15, 20, 25, 30, 35 and 40 minutes. the results were used for plotting the survival curves. the time of the microbial death in the ampoules and capillaries at all the temperatures was the same and the ampoules were chos ... | 1982 | 7065645 |
| influence of environmental storage relative humidity on biological indicator resistance, viability, and moisture content. | the effect of environmental storage relative humidity (rh) on the moisture content, viability, and moist heat and gaseous ethylene oxide (eo) resistance of biological indicators (bis) was evaluated. no statistically significant difference was observed between the initial bacillus stearothermophilus spore population and the spore population of bis stored at 20 degrees c and 0, 20, 44, of 55% rh or under ambient, 4 degrees c, or -20 degrees c conditions after 12 months. a statistically significant ... | 1982 | 7073275 |
| the reactivation of stationary phase ribosomes from bacillus stearothermophilus. | 1982 | 7073751 | |
| crystallization of lactate dehydrogenase from bacillus stearothermophilus. | 1982 | 7077664 | |
| a new approach to sterilization conditions. the imo concept. | the sterilization requirements for medical/pharmaceutical applications are traditionally based on an extensive overkill. in the past few years, however, an evolution towards bioburden related sterilization processes has been started (f0 theory). especially manufacturers of large volume parenterals have - forced by the thermolability of the product - contributed to this development. in this paper both philosophies are combined, resulting in a concept in which the bacteriological and physical base ... | 1982 | 7099915 |
| [numerical taxonomy of a thermophilic "bacillus" species isolated from west african rice soils (author's transl)]. | fifty-seven strains of endospore-forming thermophilic bacteria, 37 of which were capable of denitrification, were isolated from rice soils of west africa. they were compared with 17 strains of similar bacteria from culture collections, utilizing a total of 123 morphological, physiological and biochemical characteristics. a numerical analysis was performed using the complete linkage-clustering method and the khi2 test. seventy-five percent (55 strains) could be included in 12 groups at a taxonomi ... | 1982 | 7103312 |
| mössbauer spectroscopic evidence for the conversion of [4 fe--4 s] clusters in bacillus stearothermophilus ferredoxin into [3 fe--3 s] clusters. | 1982 | 7106280 | |
| the interaction of tryptophanyl-trna synthetase with the triazine dye brown mx-5br. | brown mx-5br specifically and irreversibly inactivates tryptophanyl-trna synthetase from bacillus stearothermophilus at ph 8.5. the enzyme is protected from inactivation by the substrates tryptophan and atp and to lesser extents by adp, amp, the product inorganic pyrophosphate and other nucleotides such as gtp. the kd of the pure reactive dye for the enzyme was measured to be 6.7 x 10(-6) m. the km values of the two substrates tryptophan and mgatp were found to be 1 x 10(-5) m and 5 x 10(-5) m r ... | 1982 | 7117237 |
| effect of environmental conditions during heating on commercial spore strip performance. | commercial biological indicator spore strips in glassine envelopes, produced by three manufacturers, were evaluated by fraction-negative procedures after being heated at 121.0 +/- 0.05 degrees c. only one type of spore strip met the manufacturer's specifications. the strips of one manufacturer were further evaluated by fraction-negative and survivor curve-plate count procedures after being heated under several conditions (enclosed in glassine envelopes, in trypticase soy broth plus 0.0015% bromo ... | 1982 | 7125646 |
| rapid screening assay for beta-lactam antibiotics in milk: collaborative study. | a 15 min assay for beta-lactam antibiotics has been used by dairies for several years as a screening procedure for testing milk tankers before they unload. the test is based on a competition between 14c-penicillin and beta-lactam antibiotics in the milk samples for sites on a microbial cell wall that specifically binds beta-lactam. in a collaborative study, 11 laboratories correctly distinguished 10 coded zero penicillin g samples and 10 coded 0.01 iu/ml samples. the proposed test is qualitative ... | 1982 | 7130091 |
| qualitative ampule and multitest for beta-lactam residues in fluid milk products: collaborative study. | a collaborative study was performed on a rapid bacillus stearothermophilus agar diffusion ampule method to detect low levels of penicillin g in 7 types of fluid milk products. a multitest technique for processing a large number of samples simultaneously was also studied. slight modifications were made in the original method to establish more uniformity and to eliminate doubtful responses by specifying a confirmation procedure. twenty samples spiked with penicillin g (0.000 to 0.008 iu/ml) and te ... | 1982 | 7130092 |
| bacillus stearothermophilus disc assay for detection of inhibitors in milk: collaborative study. | a 2-part (a and b) collaborative study was conducted on a bacillus stearothermophilus paper disc (12.7 mm) method to detect residual inhibitors in milk. the 18 participating collaborators assayed raw milk samples spiked with a beta-lactam (penicillin g). of the 18 collaborators, 14 participated in part a and 16 in part b. part a demonstrated that either antibiotic medium no. 4 or pm indicator agar is suitable for use in the assay. the lowest concentration detectable, not significantly different ... | 1982 | 7130093 |
| use of chromatofocusing in the purification of tryptophanyl-trna synthetase from bacillus stearothermophilus. | 1982 | 7149233 | |
| phosphoglycerate kinase from bacillus stearothermophilus. | 1982 | 7154941 |